技术领域technical field
本发明涉及细菌生物膜孵育系统技术领域，具体涉及一种灌流式膀胱细菌生物膜动态孵育模拟系统。The invention relates to the technical field of bacterial biofilm incubation systems, in particular to a perfusion bladder bacterial biofilm dynamic incubation simulation system.
背景技术Background technique
细菌生物膜(Bacteria lbiofilm，BF)是细菌为了生存，于介质表面以群体的方式包埋于胞外多聚物(extraceUular polymeric substances，EPS)组成的基质网中，为自己构建了一个功能性的“保护伞系统”。BF由胞外多聚物、细菌(膜内细菌或生物膜细菌)和水分三部分组成。胞外多聚物是维系其结构和功能的基础，由细菌分泌的多糖蛋白复合物、细菌代谢产物和吸附周围环境物质等组成。Bacteria biofilm (Bacteria lbiofilm, BF) is bacteria embedded in the matrix network composed of extracellular polymeric substances (extraceUular polymeric substances, EPS) in the form of groups on the surface of the medium in order to survive, and build a functional environment for themselves. "Umbrella System". BF consists of three parts: extracellular polymers, bacteria (intramembrane bacteria or biofilm bacteria) and water. Extracellular polymers are the basis for maintaining their structure and function, and are composed of polysaccharide-protein complexes secreted by bacteria, bacterial metabolites, and adsorbed environmental substances.
导尿管相关性尿路感染(CAUTIs)是院内感染最重要的原因之一。导尿管表面生物膜形成是引起CAUTIs致病和致耐药最为重要的原因。Catheter-associated urinary tract infections (CAUTIs) are one of the most important causes of nosocomial infections. Biofilm formation on the catheter surface is the most important cause of CAUTIs pathogenesis and drug resistance.
导尿管作为医源性的植入物，一方面突破人体正常的生理防御机制，增加了外源性致病菌或条件致病菌感染的机会，另一方面作为异物成为细菌粘附和定植，以及进一步形成BF的合适载体。研究表明，BF所提供的保护性微环境与60％－85％的植入物体内病原菌感染有密不可分的关系，既是细菌感染急性发作或慢性反复的原因，又能诱发免疫反应，形成过度促炎症效应危害宿主。As an iatrogenic implant, on the one hand, the urinary catheter breaks through the normal physiological defense mechanism of the human body and increases the chance of infection by exogenous pathogenic bacteria or opportunistic pathogenic bacteria; on the other hand, as a foreign body, it becomes bacterial adhesion and colonization , and a suitable carrier for further formation of BF. Studies have shown that the protective microenvironment provided by BF is closely related to 60%-85% of pathogenic bacteria infection in implants. The inflammatory effects harm the host.
近年来，细菌感染造成的严重CAUTIs不良事件或病死率逐年增高，已造成了严重的卫生经济负担，其防治工作任重而道远。In recent years, serious CAUTIs adverse events or fatality rate caused by bacterial infection have been increasing year by year, which has caused a serious health and economic burden, and the prevention and treatment work has a long way to go.
研究尿源性BF首先需要构建模拟膀胱内环境的BF孵育系统。既往研究的BF形成实验大多采用静置培养方法(如试管、多孔培养板等)，细菌在静止培养条件下于介质表面形成BF，干预因素也相对简单。细菌不断生长繁殖、BF不断形成均会消耗能量，培养基内的营养物质分布不平衡或逐渐匮乏，细菌代谢产物不断积累等均会严重影响细菌及其生物膜的正常生长。因而，不同的培养条件(如温度、时间、营养条件、流体力学、介质表面类等)，培养基加入方式(补加或流加)、更新周期等均可能会影响BF的形态和功能。To study urinary-derived BF, it is first necessary to construct a BF incubation system that simulates the environment in the bladder. Most of the BF formation experiments studied in the past used static culture methods (such as test tubes, multi-well culture plates, etc.), and bacteria formed BF on the surface of the medium under static culture conditions, and the intervention factors were relatively simple. The continuous growth and reproduction of bacteria and the continuous formation of BF will consume energy, the distribution of nutrients in the medium is unbalanced or gradually lacking, and the accumulation of bacterial metabolites will seriously affect the normal growth of bacteria and their biofilms. Therefore, different culture conditions (such as temperature, time, nutritional conditions, fluid mechanics, medium surface type, etc.), medium addition methods (supplementation or fed-batch), renewal cycle, etc. may affect the morphology and function of BF.
目前已有的孵育系统多为静态孵育，由于膀胱内尿流环境始终处于动态，因此对于膀胱细菌而言，静态孵育的研究方法无法真实的反应其生长环境，从而影响最终分析判断。At present, most of the existing incubation systems are static incubation. Since the urine flow environment in the bladder is always dynamic, for bladder bacteria, the research method of static incubation cannot truly reflect the growth environment, thus affecting the final analysis and judgment.
有鉴于此，特提出本发明。In view of this, the present invention is proposed.
发明内容Contents of the invention
为了解决现有技术存在的问题，本发明的目的在于，提供一种灌流式膀胱细菌生物膜动态孵育模拟系统，以解决现有技术无法真实反应膀胱细菌生长环境的问题。In order to solve the problems in the prior art, the object of the present invention is to provide a perfusion bladder biofilm dynamic incubation simulation system to solve the problem that the prior art cannot truly reflect the growth environment of bladder bacteria.
本发明解决上述技术问题的技术方案如下：The technical scheme that the present invention solves the problems of the technologies described above is as follows:
一种灌流式膀胱细菌生物膜动态孵育模拟系统，包括：储尿器、蠕动泵、孵育盒、集尿器和连接管，所述连接管将所述储尿器、所述蠕动泵、所述孵育盒和所述集尿器依次连接；其中，所述孵育盒包括盒体以及与所述盒体连接的盒盖，所述盒体的两侧分别开设有进液孔和出液孔，所述进液孔通过所述连接管与所述蠕动泵的出口连接，所述出液孔通过所述连接管与所述集尿器连接。A perfusion bladder bacterial biofilm dynamic incubation simulation system, comprising: a urine storage device, a peristaltic pump, an incubation box, a urine collector and a connecting pipe, the connecting pipe connects the urinary storage device, the peristaltic pump, the The incubation box and the urine collector are sequentially connected; wherein, the incubation box includes a box body and a lid connected to the box body, and the two sides of the box body are respectively provided with a liquid inlet hole and a liquid outlet hole. The liquid inlet hole is connected to the outlet of the peristaltic pump through the connecting pipe, and the liquid outlet hole is connected to the urine collector through the connecting pipe.
本发明通过蠕动泵将储存于储尿器中的尿液不断泵送至孵育盒中，同时通过开设于孵育盒盒体两侧的进液孔和出液孔，将来自储尿器中的尿液在孵育盒内形成连续的动态流体环境，实现膀胱细菌生物膜孵育条件的动态化，更加真实地模拟出膀胱细菌的生长环境。本发明实现了模拟膀胱内环境的孵育条件持续化和可变化，使得实验干预措施动态化和多样化成为可能。The present invention continuously pumps the urine stored in the urine storage device into the incubation box through a peristaltic pump, and simultaneously passes the urine from the urine storage device through the liquid inlet hole and the liquid outlet hole opened on both sides of the incubation box body. The liquid forms a continuous dynamic fluid environment in the incubation box, realizing the dynamics of the incubation conditions of the bladder bacteria biofilm, and more realistically simulating the growth environment of the bladder bacteria. The invention realizes continuous and changeable incubation conditions for simulating the environment in the bladder, making it possible to make the experimental intervention measures dynamic and diversified.
进一步地，在本发明较佳的实施例中，上述进液孔距离所述盒体底部的高度大于所述出液孔距离所述盒体底部的高度。Further, in a preferred embodiment of the present invention, the height of the liquid inlet hole from the bottom of the box body is greater than the height of the liquid outlet hole from the bottom of the box body.
本发明通过在孵育盒盒体的两侧设置进液孔和出液孔并且进液孔的高度高于出液孔，形成具有高度落差的流入通道和流出通道，使得孵育盒内具有稳定体积的人工尿液，并能够有效防止反流。In the present invention, liquid inlet holes and liquid outlet holes are arranged on both sides of the incubation box body, and the height of the liquid inlet holes is higher than that of the liquid outlet holes to form an inflow channel and an outflow channel with a height difference, so that the incubation box has a stable volume. Artificial urine, and can effectively prevent reflux.
进一步地，在本发明较佳的实施例中，上述孵育盒包括两个沿水平方向间隔设置的所述进液孔以及两个沿水平方向间隔设置的所述出液孔。Further, in a preferred embodiment of the present invention, the above-mentioned incubation box includes two said liquid inlet holes arranged at intervals along the horizontal direction and two said liquid outlet holes arranged at intervals along the horizontal direction.
进一步地，在本发明较佳的实施例中，上述孵育盒还包括分别与所述进液孔和所述出液孔配合的穿板直通接头，所述穿板直通接头与所述连接管连接。Further, in a preferred embodiment of the present invention, the above-mentioned incubating box further includes plate-through connectors respectively matched with the liquid inlet holes and the liquid outlet holes, and the plate-through connectors are connected to the connecting pipes .
进一步地，在本发明较佳的实施例中，上述孵育盒还包括多个分隔板，多个所述分隔板沿所述进液孔与所述出液孔连线的方向设置于所述盒体内。Further, in a preferred embodiment of the present invention, the above-mentioned incubating box further includes a plurality of partition plates, and the plurality of partition plates are arranged on the line connecting the liquid inlet hole and the liquid outlet hole. inside the box.
本发明在孵育盒内设置分隔板，将盒体内部划分为多个区域，保证人工尿液均匀分布，使孵育盒内更加接近膀胱内部的真实环境。In the present invention, a partition plate is arranged in the incubation box to divide the inside of the box body into multiple areas, so as to ensure the uniform distribution of artificial urine and make the inside of the incubation box closer to the real environment inside the bladder.
进一步地，在本发明较佳的实施例中，上述孵育盒为免疫组化湿盒。Further, in a preferred embodiment of the present invention, the above-mentioned incubation box is an immunohistochemical wet box.
更优选地，免疫组化湿盒为黑色不透光盒子，以模拟出人体膀胱内的黑暗环境，使得整个模拟系统更加靠近真实的人体膀胱内环境。在实验时，整套模拟系统置于37℃的恒温孵育箱内，形成与人体相同的温度环境。More preferably, the immunohistochemical wet box is a black light-proof box to simulate the dark environment in the human bladder, so that the whole simulation system is closer to the real environment in the human bladder. During the experiment, the entire simulated system was placed in a constant temperature incubator at 37°C to form the same temperature environment as the human body.
进一步地，在本发明较佳的实施例中，上述储尿器的出口通过所述连接管与所述蠕动泵的入口连接，所述蠕动泵的出口通过所述连接管与所述孵育盒的入口连接，所述孵育盒的出口通过所述连接管与所述集尿器的入口连接。Further, in a preferred embodiment of the present invention, the outlet of the above-mentioned urine reservoir is connected to the inlet of the peristaltic pump through the connecting pipe, and the outlet of the peristaltic pump is connected to the inlet of the incubation box through the connecting pipe. The inlet is connected, and the outlet of the incubation box is connected with the inlet of the urine collector through the connecting pipe.
进一步地，在本发明较佳的实施例中，上述储尿器和所述集尿器均为带密封塞的玻璃三角烧瓶，所述密封塞与所述连接管连接。Further, in a preferred embodiment of the present invention, both the above-mentioned urine storage container and the urine collector are glass Erlenmeyer flasks with sealing plugs, and the sealing plugs are connected to the connecting pipes.
进一步地，在本发明较佳的实施例中，上述连接管为硅胶管。Further, in a preferred embodiment of the present invention, the above connecting tube is a silicone tube.
本发明具有以下有益效果：The present invention has the following beneficial effects:
(1)本发明建立动态平稳的流体环境，能够更好地模拟尿液流动的状态，更加符合膀胱内尿流环境，能够为膀胱细菌提供更加真实的生长环境，为医学研究提供更加可靠的信息。(1) The present invention establishes a dynamic and stable fluid environment, which can better simulate the state of urine flow, which is more in line with the urinary flow environment in the bladder, can provide a more realistic growth environment for bladder bacteria, and provide more reliable information for medical research .
(2)本发明不仅可以保证人工尿液作为连续介质产生连续的流动状态，而且还可以以模拟出低流体剪切力条件，有利于浮游菌作用于介质表面。(2) The present invention can not only ensure that the artificial urine produces a continuous flow state as a continuous medium, but also can simulate low fluid shear force conditions, which is beneficial to the action of planktonic bacteria on the surface of the medium.
(3)本发明的流量、流速均可调节，可以控制进入系统的人工尿总量和速度，从而可以模拟生理状态的尿量增多或减少的状态(补充或缺乏水分)，昼多夜少的不同节律，可以模拟多尿或少尿的病理状态，以及模拟持续感染人工尿环境。(3) The flow rate and flow rate of the present invention can be adjusted, and the total amount and speed of artificial urine entering the system can be controlled, so that the state of increased or decreased urine output in a physiological state can be simulated (replenishment or lack of moisture), day and night Different rhythms can simulate the pathological state of polyuria or oliguria, as well as simulate the artificial urine environment of persistent infection.
(4)本发明可以实现实验干预条件如尿液葡萄糖浓度、PH值、盐离子浓度等动态化调整，实用性强。(4) The present invention can realize dynamic adjustment of experimental intervention conditions such as urine glucose concentration, pH value, salt ion concentration, etc., and has strong practicability.
(5)本发明的全部组件均属于低值耗材，拆装更换十分方便，并可反复消毒，重复使用。(5) All components of the present invention are low-value consumables, which are very convenient to disassemble and replace, and can be repeatedly sterilized and reused.
(6)本发明使用的全部材料安全可靠，无细胞毒性，十分符合模拟体外微生物实验条件。(6) All the materials used in the present invention are safe, reliable, non-cytotoxic, and fully meet the conditions of simulated in vitro microbial experiments.
附图说明Description of drawings
图1为本发明实施例的结构示意图；Fig. 1 is the structural representation of the embodiment of the present invention;
图2为本发明实施例的孵育盒的结构示意图；Fig. 2 is the structural representation of the incubation box of the embodiment of the present invention;
图3为本发明实施例的孵育盒的结构示意图；Fig. 3 is the structural representation of the incubation box of the embodiment of the present invention;
图4为本发明实施例的穿板直通接头的结构示意图。Fig. 4 is a schematic structural diagram of a through-board straight-through joint according to an embodiment of the present invention.
图中箭头表示尿液的流动方向。Arrows in the figure indicate the flow direction of urine.
图中：100－动态孵育模拟系统；110－储尿器；120－蠕动泵；130－孵育盒；131－盒体；132－盒盖；133－进液孔；134－出液孔；135－分隔板；140－集尿器；150－连接管；160－穿板直通接头；161－第一部分；162－第二部分；163－第三部分。In the figure: 100-dynamic incubation simulation system; 110-urine storage device; 120-peristaltic pump; 130-incubation box; 131-box body; 132-box cover; 133-liquid inlet hole; 134-liquid outlet hole; 135- Partition plate; 140-urine collector; 150-connecting pipe; 160-through-board joint; 161-first part; 162-second part; 163-third part.
具体实施方式detailed description
以下结合附图对本发明的原理和特征进行描述，所举实例只用于解释本发明，并非用于限定本发明的范围。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市售购买获得的常规产品。The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.
实施例Example
请参照图1，灌流式膀胱细菌生物膜动态孵育模拟系统100包括：储尿器110、蠕动泵120、孵育盒130、集尿器140和连接管150。连接管150将储尿器110、蠕动泵120、孵育盒130和集尿器140依次连接。具体地，储尿器110的出口通过连接管150与蠕动泵120的入口连接，蠕动泵120的出口通过连接管150与孵育盒130的入口连接，孵育盒130的出口通过连接管150与集尿器140的入口连接。在本实施例中，连接管150为硅胶管。本发明的连接管150的数量没有具体限制，根据具体情况适应性调整。Please refer to FIG. 1 , the perfusion bladder biofilm dynamic incubation simulation system 100 includes: a urine reservoir 110 , a peristaltic pump 120 , an incubation box 130 , a urine collector 140 and a connecting pipe 150 . The connecting pipe 150 connects the urine storage 110 , the peristaltic pump 120 , the incubation box 130 and the urine collector 140 in sequence. Specifically, the outlet of the urine storage device 110 is connected to the inlet of the peristaltic pump 120 through the connecting pipe 150, the outlet of the peristaltic pump 120 is connected to the inlet of the incubating box 130 through the connecting pipe 150, and the outlet of the incubating box 130 is connected to the urine collecting box through the connecting pipe 150. The inlet connection of the device 140. In this embodiment, the connecting tube 150 is a silicone tube. The number of connecting pipes 150 in the present invention is not specifically limited, and can be adjusted adaptively according to specific conditions.
请参照图1，储尿器110包括容器本体和设置于容器开口处的密封塞。密封塞具有与连接管150相配合的通孔，连接管150穿过密封塞将储尿器110与蠕动泵120连接。储尿器110用于储存人工尿液，为孵育盒130提供尿流。在本实施例中，储尿器110为带密封塞的玻璃三角烧瓶，其容积为500mL。在本发明的其他实施例中，储尿器110也可以是其他可密封容器。集尿器140与储尿器110结构相同，也为带密封塞的玻璃三角烧瓶，密封塞与连接管150连接。Please refer to FIG. 1 , the urine storage device 110 includes a container body and a sealing plug disposed at the opening of the container. The sealing plug has a through hole matched with the connecting tube 150 , and the connecting tube 150 passes through the sealing plug to connect the urine reservoir 110 with the peristaltic pump 120 . The urine reservoir 110 is used to store artificial urine and provide urine flow for the incubation box 130 . In this embodiment, the urine storage container 110 is a glass Erlenmeyer flask with a sealing stopper, and its volume is 500 mL. In other embodiments of the present invention, the urine storage container 110 may also be other sealable containers. The urine collection device 140 has the same structure as the urine storage device 110, and is also a glass Erlenmeyer flask with a sealing plug, which is connected to the connecting pipe 150.
请参照图1，蠕动泵120通过连接管150分别与储尿器110和孵育盒130连接，其作用在于将储尿器110中的人工尿液泵送至孵育盒130中。在本实施例中，采用的蠕动泵120为卡默尔品牌，其泵体小巧，减少使用空间，通过旋钮手动调速和计量，无门阀和密封件，进出水管更换方便，而且其泵头为卡扣设计，易于拆装，清理方便，重复精度高，三轮滚动，脉动小，液体在泵管内运行，接触泵管内壁，不接触泵体，安全无污染。在本发明的其他实施例中，也可以使用其他品牌的蠕动泵120。本发明未对蠕动泵120的结构进行改进，因此其具体结构在此不做说明，本发明使用的蠕动泵120为现有蠕动泵。Please refer to FIG. 1 , the peristaltic pump 120 is respectively connected to the urine storage 110 and the incubation box 130 through the connecting tube 150 , and its function is to pump the artificial urine in the urine storage 110 to the incubation box 130 . In this embodiment, the peristaltic pump 120 used is the Carmel brand. Its pump body is small and exquisite, which reduces the use of space. The speed and metering are manually adjusted through the knob. There are no gate valves and seals. The water inlet and outlet pipes are easy to replace. Buckle design, easy to disassemble, easy to clean, high repeatability, three-wheel rolling, small pulsation, the liquid runs in the pump tube, contacts the inner wall of the pump tube, does not touch the pump body, safe and pollution-free. In other embodiments of the present invention, other brands of peristaltic pumps 120 may also be used. The present invention does not improve the structure of the peristaltic pump 120, so its specific structure will not be described here. The peristaltic pump 120 used in the present invention is an existing peristaltic pump.
请参照图2和图3，孵育盒130包括盒体131、与盒体131连接的盒盖132以及设置于盒体131的多个分隔板135。盒体131的两侧分别开设有进液孔133和出液孔134。进液孔133通过连接管150与蠕动泵120的出口连接。出液孔134通过连接管150与集尿器140连接。进液孔133距离盒体131底部的高度大于出液孔134距离盒体131底部的高度，由此可以形成具有高差的流入通道和流出通道，避免尿液反流。在本实施例中，进液孔133和出液孔134均为两个，并且彼此对应，而且两个进液孔133和两个出液孔134均沿水平方向彼此间隔设置。在一个优选的实施方式中，进液孔133的孔高为2cm(距离盒体131底部)，两孔距离9cm。出液孔134的孔高为1cm(距离盒体131底部)，两孔距离9cm。与两个进液孔133对应的两个连接管150通过三通接头与连接蠕动泵120的连接管150连接，同理，出液孔134也是如此。Referring to FIG. 2 and FIG. 3 , the incubation box 130 includes a box body 131 , a box cover 132 connected to the box body 131 , and a plurality of partition plates 135 disposed on the box body 131 . Two sides of the box body 131 are respectively provided with a liquid inlet hole 133 and a liquid outlet hole 134 . The liquid inlet hole 133 is connected with the outlet of the peristaltic pump 120 through a connecting pipe 150 . The liquid outlet hole 134 is connected with the urine collector 140 through a connecting pipe 150 . The height of the liquid inlet hole 133 from the bottom of the box body 131 is greater than the height of the liquid outlet hole 134 from the bottom of the box body 131, thereby forming an inflow channel and an outflow channel with a height difference to avoid backflow of urine. In this embodiment, there are two liquid inlet holes 133 and two liquid outlet holes 134 corresponding to each other, and the two liquid inlet holes 133 and the two liquid outlet holes 134 are arranged at intervals along the horizontal direction. In a preferred embodiment, the hole height of the liquid inlet hole 133 is 2 cm (from the bottom of the box body 131 ), and the distance between the two holes is 9 cm. The hole height of the liquid outlet hole 134 is 1 cm (from the bottom of the box body 131), and the distance between the two holes is 9 cm. The two connecting pipes 150 corresponding to the two liquid inlet holes 133 are connected to the connecting pipe 150 connected to the peristaltic pump 120 through a three-way joint, and the same is true for the liquid outlet hole 134 .
如图2所示，多个分隔板135沿进液孔133与出液孔134连线的方向设置于盒体131内。图2中示出的实施例具有4个分隔板135，在本发明的其他实施例中，可以是3个也可以是更多个，具体个数可以根据孵育盒130大小进行调整。As shown in FIG. 2 , a plurality of partition plates 135 are disposed in the box body 131 along the direction connecting the liquid inlet hole 133 and the liquid outlet hole 134 . The embodiment shown in FIG. 2 has four partition plates 135 , in other embodiments of the present invention, there may be three or more, and the specific number can be adjusted according to the size of the incubation box 130 .
请参照图4，孵育盒130还包括分别与进液孔133和出液孔134配合的穿板直通接头160，穿板直通接头160与连接管150连接。穿板直通接头160大致为直线型的圆管，其一端与连接管150连接，另一端保持在孵育盒130内，其连接连接管150和孵育盒130的作用。在本实施例中，穿板直通接头160的结构如图4所示，其主要包括三部分：第一部分161大致呈圆柱体结构，其表面设有螺纹，该部分与连接管150连接；第二部分162为中间的环形凸起，在安装时保持在盒体131的外侧，其到固定的作用；第三部分163也大致呈圆柱体结构，其表面光滑，该部分与进液孔133或出液孔134配合，穿过盒体131的侧壁。Please refer to FIG. 4 , the incubation box 130 further includes through-board straight connectors 160 respectively matched with the liquid inlet hole 133 and the liquid outlet hole 134 , and the through-board straight-through connector 160 is connected with the connecting pipe 150 . The through-board straight-through joint 160 is roughly a straight circular tube, one end of which is connected to the connecting tube 150 , and the other end is kept in the incubation box 130 , which serves to connect the connecting tube 150 and the incubation box 130 . In this embodiment, the structure of the through-board straight-through joint 160 is shown in FIG. 4 , which mainly includes three parts: the first part 161 is roughly a cylindrical structure, and its surface is provided with threads, and this part is connected with the connecting pipe 150; Part 162 is an annular protrusion in the middle, which is kept on the outside of the box body 131 during installation, and it has a fixed effect; the third part 163 is also roughly cylindrical in structure, and its surface is smooth, and this part is connected with the liquid inlet 133 or the outlet The liquid hole 134 fits through the side wall of the box body 131 .
在本发明的另一个实施例中，孵育盒130可以采用免疫组化湿盒，免疫组化湿盒的盒身两侧开设进液孔133和出液孔134，并且通过穿板直通接头160与其他部件连接。In another embodiment of the present invention, the incubation box 130 can be an immunohistochemical wet box, and the two sides of the box body of the immunohistochemical wet box are provided with a liquid inlet 133 and a liquid outlet 134, and through the through-plate through connector 160 and Other components are connected.
下面对本发明的工作原理和操作流程进行说明。The working principle and operation process of the present invention will be described below.
本发明采用穿板直通接头160、三通接头、硅胶管、蠕动泵120、孵育盒130、储尿器110和集尿器140，模拟膀胱内尿流环境持续尿流状态，构建了体外灌流式灌流式膀胱细菌生物膜动态孵育模拟系统100。使用时，开启电源，蠕动泵120正常运转，将人工尿液缓缓泵入孵育盒130，孵育盒130内人工尿液蓄积正常，保持一定体积，例如约500ml，形成动态连续流动环境。实验完成后，关闭电源，系统停止运转。本发明利用蠕动泵120将储尿器110中的人工尿液经流入通道主动泵入孵育盒130，形成孵育微环境，人工尿液经流出通道缓慢流出，最终到达集尿器140。建立连续的流动环境，实现试验条件的动态化。The present invention adopts through-board straight-through joint 160, tee joint, silicone tube, peristaltic pump 120, incubation box 130, urine storage device 110 and urine collection device 140 to simulate the continuous urine flow state in the urinary flow environment in the bladder and construct an in vitro perfusion type. A perfusion bladder biofilm dynamic incubation simulation system 100 . When in use, turn on the power, the peristaltic pump 120 operates normally, and slowly pump the artificial urine into the incubation box 130. The artificial urine in the incubation box 130 accumulates normally and maintains a certain volume, such as about 500ml, forming a dynamic continuous flow environment. After the experiment is completed, turn off the power and the system stops. In the present invention, the peristaltic pump 120 is used to actively pump the artificial urine in the urine storage device 110 into the incubation box 130 through the inflow channel to form an incubation microenvironment. The artificial urine slowly flows out through the outflow channel and finally reaches the urine collector 140 . Establish a continuous flow environment to achieve dynamic test conditions.
具体操作流程如下：The specific operation process is as follows:
(1)全部组件高温消毒后(蠕动泵120泵体除外)备用。开始实验时，戴无菌手套按照顺序连接各组件，检查无误。(1) After high-temperature disinfection of all components (except for the pump body of the peristaltic pump 120), they are ready for use. When starting the experiment, wear sterile gloves and connect the components in order to check that they are correct.
(2)于储尿器110装满人工尿液约500ml，开启蠕动泵120电源，人工尿液自流入口缓缓泵入孵育盒130。(2) Fill the urine storage device 110 with about 500 ml of artificial urine, turn on the power of the peristaltic pump 120 , and slowly pump the artificial urine into the incubation box 130 from the inlet.
(3)孵育盒130的流入、出口高度落差为1cm，始终保持其内约500ml稳定体积的人工尿液。(3) The height difference between the inflow and the exit of the incubation box 130 is 1 cm, and artificial urine with a stable volume of about 500 ml is always maintained therein.
(4)于孵育盒130的流出通道观察人工尿液滴出的速度，调节蠕动泵120(流速范围4－65ml/min)，到达实验需要的流量和流速。(4) Observe the dripping speed of artificial urine in the outflow channel of the incubation box 130, and adjust the peristaltic pump 120 (flow rate range 4-65ml/min) to reach the required flow rate and flow rate for the experiment.
(5)定时更换储尿器110和集尿器140，保证系统通常运行。(5) Regularly replace the urine storage device 110 and the urine collection device 140 to ensure the normal operation of the system.
(6)停止实验，关闭电源，拆除组件，消毒备用。(6) Stop the experiment, turn off the power, remove the components, and sterilize for standby.
综上所述，本发明的灌流式灌流式膀胱细菌生物膜动态孵育模拟系统100由易于获取的实验室器材，通过自行设计和组装而成，制备出了尽可能模拟人体膀胱内尿流环境的细菌生物膜动态模型，并可以实现连续或变化的干预因素，如持续人工尿液环境等观察细菌生物膜形成的生长发育特点。本发明的动态孵育模拟系统100经济适用、组装简便、重复性好、安全有效。In summary, the perfusion-perfusion bladder bacterial biofilm dynamic incubation simulation system 100 of the present invention is designed and assembled from easily obtained laboratory equipment, and has prepared a urine flow environment that simulates the urinary flow environment in the human bladder as much as possible. The dynamic model of bacterial biofilm can realize continuous or changing intervention factors, such as continuous artificial urine environment, etc., to observe the growth and development characteristics of bacterial biofilm formation. The dynamic incubation simulation system 100 of the present invention is economical and applicable, easy to assemble, good in repeatability, safe and effective.
本发明的“孵育系统”具有极高的临床价值；同时由于导尿管相关性尿路感染、泌尿系植入物感染、结石形成等发病率高且上升趋势，因此基于该“孵育系统”模拟膀胱内环境的细菌生物膜系列研究及成果也具有极高的经济效益与市场前景。The "incubation system" of the present invention has extremely high clinical value; at the same time, due to the high incidence and rising trend of catheter-related urinary tract infection, urinary implant infection, stone formation, etc., based on the "incubation system" simulation The serial research and results of bacterial biofilm in the bladder environment also have extremely high economic benefits and market prospects.
以上所述仅为本发明的较佳实施例，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection of the present invention. within range.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710858891.8ACN107418891A (en) | 2017-09-20 | 2017-09-20 | A kind of perfusion type bladder bacterial biof iotalm dynamic is incubated simulation system |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710858891.8ACN107418891A (en) | 2017-09-20 | 2017-09-20 | A kind of perfusion type bladder bacterial biof iotalm dynamic is incubated simulation system |
| Publication Number | Publication Date |
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| CN107418891Atrue CN107418891A (en) | 2017-12-01 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710858891.8APendingCN107418891A (en) | 2017-09-20 | 2017-09-20 | A kind of perfusion type bladder bacterial biof iotalm dynamic is incubated simulation system |
| Country | Link |
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| CN (1) | CN107418891A (en) |
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| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | Application publication date:20171201 | |
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