技术领域technical field
本发明属于高分子、生物流体在微尺度下的混合技术领域，特别涉及一种可实现高效混合的微流体芯片。The invention belongs to the technical field of mixing macromolecules and biological fluids at a microscale, and in particular relates to a microfluidic chip capable of achieving high-efficiency mixing.
背景技术Background technique
目前,传统微流控芯片虽然应用广泛,大部分微流控生物芯片和微机电系统(MEMS)已经用于生物学和医学研究以及芯片实验室(Lab-on-chip)的控制等领域。但多数仅用于单一组分微流体的输送和控制，对于两种组分以上流体的混合仍然存在混合效率低，时间长的缺点，无法满足科学实验要求多种微流体快速混合的需要。At present, although traditional microfluidic chips are widely used, most microfluidic biochips and MEMS have been used in biological and medical research, as well as the control of Lab-on-chip. However, most of them are only used for the delivery and control of single-component microfluids. For the mixing of two or more fluids, there are still disadvantages of low mixing efficiency and long time, which cannot meet the needs of scientific experiments that require rapid mixing of multiple microfluids.
中国专利文献中，公开号为CN102527280A、发明名称为“一种微混合和微反应装置”的发明专利公开了一种技术方案，包括微流控芯片，由刻有微通道的聚二甲基硅烷薄层与玻璃片键合而成，和芯片夹，用于夹持所述微流控芯片，所述微流道包括两条溶液入口通道和一条溶液出口通道，每条通道由一宽通道和一窄通道贯通连接形成，且三个窄通道均与一混合腔贯通连接，从而使三条通道相互连通。上述技术方案虽然实现了两种以上组分的微混合，但是存在混合不均匀、混合时间长，混合质量差等缺点。In the Chinese patent literature, the invention patent with the publication number CN102527280A and the invention title "a micro-mixing and micro-reaction device" discloses a technical solution, including a microfluidic chip made of polydimethylsilane with microchannels The thin layer is bonded with a glass sheet, and a chip clamp is used to clamp the microfluidic chip. The microfluidic channel includes two solution inlet channels and one solution outlet channel, and each channel consists of a wide channel and a A narrow passage is formed through connection, and the three narrow passages are connected with a mixing chamber, so that the three passages communicate with each other. Although the above-mentioned technical scheme realizes the micro-mixing of two or more components, there are disadvantages such as uneven mixing, long mixing time, and poor mixing quality.
发明内容Contents of the invention
为了解决上述技术问题，本发明提供一种可实现高效混合的微流体芯片，目的是满足多组分高分子流体充分混合的要求，提高混合质量。In order to solve the above-mentioned technical problems, the present invention provides a microfluidic chip capable of achieving high-efficiency mixing, the purpose of which is to meet the requirements of sufficient mixing of multi-component polymer fluids and improve the mixing quality.
为达到上述目的，本发明的技术方案如下：To achieve the above object, the technical scheme of the present invention is as follows:
一种可实现高效混合的微流体芯片，包括基板、覆盖在基板上下的上玻璃片、下玻璃片，所述基板上设有不同几何结构的通道，所述通道包括入口通道、直通道和出口通道；所述通道均匀分布在基板上。A microfluidic chip capable of high-efficiency mixing, including a substrate, an upper glass sheet covering the upper and lower sides of the substrate, and a lower glass sheet. The substrate is provided with channels of different geometric structures, and the channels include inlet channels, straight channels and outlets. channels; the channels are evenly distributed on the substrate.
和现有技术相比，本方案基于多组分流体充分高效混合的构思，设置了微流体芯片基板的通道，包括均匀分布在基板上的不同几何结构的通道。通道均匀设置在基板上有利于微流体芯片受力均匀，提高基板使用寿命和减少了因基板变形引起的测量误差。不同几何结构的通道可实现不同组分液体的高效、快速混合，得到混合均匀的溶液。Compared with the prior art, this solution is based on the idea of fully and efficiently mixing multi-component fluids, and provides channels for the substrate of the microfluidic chip, including channels of different geometric structures evenly distributed on the substrate. The channels are evenly arranged on the substrate, which is beneficial to the microfluidic chip to be evenly stressed, which improves the service life of the substrate and reduces the measurement error caused by the deformation of the substrate. Channels with different geometric structures can realize efficient and rapid mixing of liquids with different components, and obtain a uniformly mixed solution.
基于上述方案，本发明还做出了如下改进：Based on the above scheme, the present invention also makes the following improvements:
所述基板上一端设有圆弧通道，作为流体A的入口通道；所述圆弧通道径向外延伸处设有流体B的入口通道，流体A、B通道构成皇冠形状。本改进方案中通过圆弧通道和圆弧外延伸通道的设计，实现流体B在进入圆弧通道时被流体A沿圆弧切线切断形成微滴液，进入直流道二者再进行进一步的混合；流体A的粘度一般高于流体B的粘度，粘度较高的流体对粘度较低流体的剪切力度较好，提高混合效率。One end of the base plate is provided with an arc channel as the inlet channel of fluid A; the radially outward extension of the arc channel is provided with an inlet channel of fluid B, and the channels of fluid A and B form a crown shape. In this improvement plan, through the design of the arc channel and the arc-extended channel, the fluid B is cut off by the fluid A along the arc tangent when entering the arc channel to form a droplet, and then enters the straight channel for further mixing; The viscosity of fluid A is generally higher than that of fluid B, and the fluid with higher viscosity has better shearing force on the fluid with lower viscosity, which improves the mixing efficiency.
所述直通道前段靠近入口通道处嵌有镶块，直通道中后段为沿基板中轴线开设、均匀分布的凹凸交错的凹凸通道。本改进方案中利用镶块和凹凸转弯通道的作用对混合液体进行充分高效的混合。The front section of the straight channel is inlaid with inserts close to the entrance channel, and the middle and rear sections of the straight channel are concave-convex channel with interlaced concave and convex evenly distributed along the central axis of the substrate. In this improvement scheme, the effect of the insert and the concave-convex turning channel is used to fully and efficiently mix the mixed liquid.
所述入口通道关于基板中轴对称，或位于基板的一侧；本改进方案中根据流体压力决定入口通道的形状，若混合量多采用流道对称的结构可以显著降低因流体压力产生的内应力。而混合量小时压力小，采用仅一侧的非对称结构时内应力也不大可满足要求。The inlet channel is symmetrical about the central axis of the substrate, or is located on one side of the substrate; in this improvement, the shape of the inlet channel is determined according to the fluid pressure, and if the mixing amount is large, the internal stress caused by the fluid pressure can be significantly reduced by adopting a symmetrical structure of the flow channel . And when the mixing amount is small, the pressure is small, and the internal stress is not large enough to meet the requirements when the asymmetric structure with only one side is adopted.
所述圆弧通道径向外延伸处设有流体C的入口通道，流体B、C通道相邻，流体A通道与流体B、C通道构成皇冠形状。本改进方案是针对两种以上组分的液体设计的，流体A可以实现对流体B、C的剪切，实现初步的混合。The inlet channel of fluid C is provided at the radially outward extension of the arc channel, the channels of fluid B and C are adjacent, and the channel of fluid A and the channels of fluid B and C form a crown shape. This improvement is designed for liquids with more than two components. Fluid A can shear fluids B and C to achieve preliminary mixing.
流体B入口通道中轴与直通道中轴夹角β范围为15゜-75゜。The angle β between the central axis of the inlet channel of fluid B and the central axis of the straight channel is in the range of 15°-75°.
圆弧通道与直通道的夹角α范围为100°-150゜。The angle α between the arc channel and the straight channel is in the range of 100°-150°.
本发明的有益效果是多种特殊几何形状结构的通道，用于控制多种液体组分导入到主通道，并且实现微液滴的切割、形成、输送和混合；可实现多组分流体充分高效的混合，进一步借助光学显微镜可以观察微流体的流动和混合情况。The beneficial effect of the present invention is that a variety of channels with special geometric shapes are used to control the introduction of various liquid components into the main channel, and realize the cutting, formation, transportation and mixing of micro-droplets; the multi-component fluid can be fully efficient The mixing of microfluids can be further observed with the help of an optical microscope to observe the flow and mixing of microfluids.
附图说明Description of drawings
为了更清楚地说明发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍。In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the description of the embodiments or the prior art.
图1为本发明实施例1微流体芯片的俯视图；Fig. 1 is the top view of the microfluidic chip of embodiment 1 of the present invention;
图2为本发明实施例1微流体芯片的A-A剖视图；Fig. 2 is the A-A sectional view of the microfluidic chip of Example 1 of the present invention;
图3为本发明实施例2微流体芯片的俯视图；Fig. 3 is a top view of the microfluidic chip of Example 2 of the present invention;
图4为本发明实施例3微流体芯片的俯视图；Fig. 4 is a top view of the microfluidic chip of Example 3 of the present invention;
图5为本发明实施例4微流体芯片的俯视图；5 is a top view of a microfluidic chip according to Embodiment 4 of the present invention;
图中，1、基板；2、上玻璃片；3、下玻璃片；4、入口通道；5、直通道；6、出口通道；40-1、40-2、流体A入口通道；41-1、41-2、流体B入口通道；42-1、42-2、流体C入口通道；50、镶块；51、凹凸通道。In the figure, 1. substrate; 2. upper glass sheet; 3. lower glass sheet; 4. inlet channel; 5. straight channel; 6. outlet channel; 40-1, 40-2, fluid A inlet channel; 41-1 , 41-2, fluid B inlet channel; 42-1, 42-2, fluid C inlet channel; 50, insert; 51, concave-convex channel.
具体实施方式detailed description
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention.
实施例1Example 1
如图1、2所示，一种可实现高效混合的微流体芯片，包括基板1、覆盖在基板上下的上玻璃片2、下玻璃片3，所述基板1上设有不同几何结构的通道，所述通道包括入口通道4、直通道5和出口通道6；所述通道均匀分布在基板1上。所述入口通4关于基板1中轴对称，所述基板1上一端设有圆弧通道，作为流体A的入口通道40-1、40-2；所述圆弧通道径向外延伸处设有流体B的入口通道41-1、41-2，流体A、B通道构成皇冠形状。所述直通道5前段靠近入口通道处嵌有镶块50，直通道5中后段为沿基板中轴线开设、均匀分布的凹凸交错的凹凸通道51；镶块50的数量可根据具体需要选择。流体B入口通道中轴与直通道中轴夹角β范围为15゜-75゜。圆弧通道的宽度范围为50-200微米。As shown in Figures 1 and 2, a microfluidic chip that can achieve efficient mixing includes a substrate 1, an upper glass sheet 2 and a lower glass sheet 3 covering the upper and lower sides of the substrate, and the substrate 1 is provided with channels of different geometric structures , the channels include an inlet channel 4 , a straight channel 5 and an outlet channel 6 ; the channels are evenly distributed on the substrate 1 . The inlet passage 4 is symmetrical about the central axis of the substrate 1, and an arc channel is provided at one end of the substrate 1 as the inlet channels 40-1 and 40-2 of the fluid A; the radially outward extension of the arc channel is provided with The inlet channels 41-1, 41-2 for fluid B, and the channels for fluid A and B form a crown shape. The front section of the straight channel 5 is inlaid with inserts 50 near the entrance channel, and the middle and rear sections of the straight channel 5 are concave-convex channels 51 that are evenly distributed along the central axis of the substrate; the number of inserts 50 can be selected according to specific needs. The angle β between the central axis of the inlet channel of fluid B and the central axis of the straight channel is in the range of 15°-75°. The width of the arc channel is in the range of 50-200 microns.
实施例2Example 2
如图3所示，与实施例1不同点在于圆弧通道径向外延伸处还设有流体C的入口通道42-1、42-2，流体B、C通道紧挨，流体C入口通道与直通道5相邻，流体A通道与流体B、C通道构成皇冠形状。流体B入口通道中轴与直通道中轴夹角β范围为15゜-75゜，流体C入口通道靠近直通道的边与直通道中轴的夹角φ范围为10゜-35゜。本实施例中流道B、C紧挨，流道B、C也可通过圆弧通道相连，二者位置也可互换，布局方式不局限于本实施例。As shown in Figure 3, the difference from Embodiment 1 is that there are also inlet passages 42-1, 42-2 for fluid C at the radially outward extension of the arc passage, and the passages for fluid B and C are next to each other, and the inlet passage for fluid C is connected to the inlet passage of fluid C. The straight channels 5 are adjacent, and the fluid A channel forms a crown shape with the fluid B and C channels. The angle β between the central axis of the inlet channel of fluid B and the central axis of the straight channel ranges from 15° to 75°, and the angle φ between the side of the inlet channel of fluid C near the straight channel and the central axis of the straight channel ranges from 10° to 35°. In this embodiment, the runners B and C are next to each other, and the runners B and C can also be connected through an arc channel, and the positions of the two can also be interchanged, and the layout is not limited to this embodiment.
实施例3Example 3
如图4所示，一种可实现高效混合的微流体芯片，包括基板1、覆盖在基板上下的上玻璃片2、下玻璃片3，所述基板1上设有不同几何结构的通道，所述通道包括入口通道4、直通道5和出口通道6；所述通道均匀分布在基板1上。所述入口通4位于基板的一侧，所述基板1上一端设有圆弧通道，作为流体A的入口通道40-1；所述圆弧通道径向外延伸处设有流体B的入口通道41-1，流体A、B通道构成半个皇冠形状。圆弧通道内侧边即半径小的边在与直通道汇合处为圆弧切线、而非圆弧。所述直通道5前段靠近入口通道处嵌有镶块50，直通道5中后段为沿基板中轴线开设、均匀分布的凹凸交错的凹凸通道51；镶块50的数量可根据具体需要选择。流体B入口通道中轴与直通道中轴夹角β范围为15゜-75゜，圆弧通道的宽度范围为50-200微米，圆弧通道内侧边与直通道的夹角α范围为100°-150゜As shown in Figure 4, a microfluidic chip that can achieve high-efficiency mixing includes a substrate 1, an upper glass sheet 2 and a lower glass sheet 3 covering the upper and lower sides of the substrate, and the substrate 1 is provided with channels of different geometric structures, so The channels include inlet channels 4, straight channels 5 and outlet channels 6; the channels are evenly distributed on the substrate 1. The inlet channel 4 is located on one side of the substrate, and an arc channel is provided at one end of the substrate 1 as an inlet channel 40-1 of fluid A; an inlet channel of fluid B is provided at the radially outward extension of the arc channel 41-1, the channels of fluid A and B form a half crown shape. The inner edge of the arc channel, that is, the side with a small radius, is the arc tangent line instead of the arc at the confluence with the straight channel. The front section of the straight channel 5 is inlaid with inserts 50 near the entrance channel, and the middle and rear sections of the straight channel 5 are concave-convex channels 51 that are evenly distributed along the central axis of the substrate; the number of inserts 50 can be selected according to specific needs. The angle β between the central axis of the inlet channel of fluid B and the central axis of the straight channel ranges from 15° to 75°, the width of the arc channel ranges from 50 to 200 microns, and the angle α between the inner side of the arc channel and the straight channel ranges from 100° -150゜
实施例4Example 4
如图5所示，与实施例3不同点在于所述圆弧通道径向外延伸处还设有流体C的入口通道42-1、42-2，流体B、C通道紧挨，流体C通道42-1与直通道5相邻，流体A通道与流体B、C通道构成半个皇冠形状。流体B入口通道中轴与直通道中轴夹角β范围为15゜-75゜，流体C入口通道靠近直通道的边与直通道中轴的夹角φ范围为10゜-35゜。本实施例中流道B、C紧挨，流道B、C也可通过圆弧通道相连，二者位置也可互换，布局方式不局限于本实施例。As shown in Figure 5, the difference from Embodiment 3 is that the inlet passages 42-1, 42-2 for fluid C are also provided at the radially outward extension of the circular arc passage, the passages of fluid B and C are next to each other, and the passages of fluid C are adjacent to each other. 42-1 is adjacent to the straight channel 5, and the fluid A channel forms a half crown shape with the fluid B and C channels. The angle β between the central axis of the inlet channel of fluid B and the central axis of the straight channel ranges from 15° to 75°, and the angle φ between the side of the inlet channel of fluid C near the straight channel and the central axis of the straight channel ranges from 10° to 35°. In this embodiment, the runners B and C are next to each other, and the runners B and C can also be connected by an arc channel, and the positions of the two can also be interchanged, and the layout is not limited to this embodiment.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710655158.6ACN107376750A (en) | 2017-08-03 | 2017-08-03 | A kind of micro-fluid chip that can be achieved efficiently to mix |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710655158.6ACN107376750A (en) | 2017-08-03 | 2017-08-03 | A kind of micro-fluid chip that can be achieved efficiently to mix |
| Publication Number | Publication Date |
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| CN107376750Atrue CN107376750A (en) | 2017-11-24 |
| Application Number | Title | Priority Date | Filing Date |
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| CN201710655158.6APendingCN107376750A (en) | 2017-08-03 | 2017-08-03 | A kind of micro-fluid chip that can be achieved efficiently to mix |
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| CN (1) | CN107376750A (en) |
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| CN113842824B (en)* | 2021-10-15 | 2023-10-10 | 西安交通大学 | Passive micromixer for enhancing mixing efficiency based on composite side wall microstructure |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | Application publication date:20171124 | |
| RJ01 | Rejection of invention patent application after publication |