A kind of minute quantity liquid precise distribution instrumentTechnical field
The present invention relates to a kind of continuous accurate distribution micro liquid and the few instrument of sample loss, it is used for available for preparationCarry out the micro-array chip of biochemical test, generate the application scenarios such as unicellular drop, trace reagent packing.
Background technology
Biochip microarray is made on diaphragm, slide, silicon chip, it is necessary to the various micro solutions dissolved with sample pointSystem corresponding point position on substrate is not selected to form.Contactless spray sample technology can realize nanoliter and micro updating volume micro liquidHigh-precision, the high multiplicity distribution of body, the producer can freely adjust sample sendout, be suitable for the life researched and developed and produced in batchesThing chip production technology.The principle of contactless spray sample method has two kinds of micro-valve control and piezo jet at present.Based on piezoelectric principleMicro liquid distribution instrument cost it is expensive；In micro- spray product based on micro-valve principle, by pressing creation part, pipeline, micro-lineCircle magnetic valve (lower abbreviation micro-valve) and shower nozzle are sequentially connected composition, and cost is more cheap.Generally directly it is connected between micro-valve and shower nozzle,To improve the degree of accuracy and the reproduction degree of spray sample amount；Spray sample amount size is by controlling micro-valve switch time and being applied to liquid in pipelineOn pressure size adjust.
Typical products based on the micro- spray principle of micro-valve have the BioJet Plus of California, USA Ai Er literary virtue BioDot companiesTMThe SmartArrayer of the rich biological group difficult to understand of series and BeijingTMProduct line.
BioJet series of products syringe pump is as liquid compression part, i.e., by injecting the propulsion of sucker rod come directlyCompression riddles the liquid in pipeline, and the pressure that liquid is born is controlled by the push-in stroke of injection sucker rod；Syringe pump is removedIn to right compression conduit outside the effect of liquid, also cleaned for carrying out the replacing of sample and passage in pipeline.Using hydraulic force actuationBioJet Plus series due to need liquid full of whole pipeline, it is necessary to sample size it is big, needed when changing sample by pipelineInterior liquid is all discharged, and the operating time is long；The liquid pearl of residual and the bubble produced are difficult to drain only in pipeline during discharge opeing, can shadowRing spray sample effect.
SmartArrayerTMProduct line is used as the power that micro liquid is ejected out of micro-valve using atmospheric pressure,So in pipeline need not it is hydraulically full, so as to saving sample；The problem of existing be employ compressed air as origin, it is multipleValve and the pressure of pressure sensor decomposition pressure adjustment unit production module generation setting pressure need the time longer and pressedPower generation module volume is larger, can only be connected micro-valve and pressing creation module by long pipe runs, secondary caused by long pipe runsRaw problem is that pressure-responsive is slower.
The content of the invention
The purpose of the present invention be it is a kind of based on continuous accurate distribution micro liquid and sample loss less, pressing creation it is rapid,The minute quantity liquid precise distribution instrument of rapid cleaning systems when changing sample.
To achieve the above object, the present invention takes technical scheme：A kind of minute quantity liquid precise distribution instrument, it includes micro-The volume displaced pump of jet mould block, Miniature precision, pressure sensor, check valve, compression pump and control circuit composition.
In practical operation, micro- jet mould block can be micro-valve and combination or the piezo jets of shower nozzle.
The volume displaced pump of Miniature precision, can be Small Plunger Pump, have on the cavity of Small Plunger Pump it is multiple andThe mouth that pump inner chamber is communicated；Can also be the combination of Miniature injection pump and a multiple-pass joint, a mouth of multiple-pass joint and miniatureSyringe pump is connected.
There is precise piston inside the volume displaced pump of Miniature precision.
The compression pump can be peristaltic pump, booster pump or air compressor.
In above-mentioned minute quantity liquid precise distribution instrument, micro- jet mould block, Miniature precision pump, pressure sensor and pressurePump is completed to cooperate by control circuit drives and control.
Other parts outside compression pump can be all fixed on mechanical fortune by above-mentioned minute quantity liquid precise distribution instrumentDynamic system is realized on the sliding block of the Z axis moved up and down, so as to shorten length of pipe, reduce the accessible volume.
The present invention is due to taking the above to design, and it has advantages below：
Precisely pressure in regulation path, improves micro liquid distribution precision；Simplify and miniaturization structure, shorten path bodyProduct, improves pressure-responsive speed；By independent exhaust bubble and liquid pearl part, shadow of the bubble to micro- spray performance is at utmost reducedRing.
Brief description of the drawings
Fig. 1 is the schematic diagram of first embodiment of the invention；
Fig. 2 is the schematic diagram of second embodiment of the invention；
Embodiment
Except special statement, the present invention uses technology and scientific words to be generic term, and usage is consistent.With the present inventionRelated patent, application for patent and pertinent literature all has been classified as the bibliography of the present invention.If term herein hasRun counter to or inconsistent place, be defined by herein with these documents.
" one " expression in text " is no less than one " or " one or multiple ".
Here two kinds of embodiments are only enumerated, the category that the present invention is protected is not limited to the technology involved by both embodimentsContent.
Fig. 1 is the schematic diagram of first embodiment of the invention.The volume displaced pump 1 of Miniature precision has three mouths, respectively and pressureForce snesor 2, micro- jet mould block 3 are connected with check valve 5；Check valve 5 is located in the volume displaced pump 1 of Miniature precision and compression pump 4Between, both are connected.The volume displaced pump 1 of Miniature precision, pressure sensor 2, the inner chamber phase of micro- jet mould block 3 and check valve 5It is logical, form path；The volume displaced pump 1 of Miniature precision is used to draw liquid, micro liquid distribution when institute is generated in the pathThe pressure needed；Pressure sensor 2 is used to monitor the pressure in the path and feeds back to control circuit 6, is controlled by control circuit 6Miniature precision volume displaced pump 1 adjusts the pressure in the path；Compression pump 4 is used for the quick raffinate by path and discharged,Improve operating efficiency；Check valve 5 is used for and compression pump 4 is used cooperatively, and its circulating direction is from compression pump 4 to Miniature precision volumeDisplacement pump 1.
The volume displaced pump 1 of Miniature precision is the mouth for having three and pump inner chamber to communicate on Small Plunger Pump, plunger pump housing；It is micro-The accurate volume displaced pump 1 of type can also be the combination of Miniature injection pump and a four-way connection, a mouth of four-way connection and micro-Type syringe pump is connected.
Other parts outside compression pump can be all fixed on machine by the minute quantity liquid precise distribution instrument of the present embodimentTool kinematic system is realized on the sliding block of the Z axis moved up and down, so as to shorten length of pipe, reduce the accessible volume.
Fig. 2 is second embodiment schematic diagram of the present invention, and the volume displaced pump 1 of Miniature precision has two mouths, respectively and pressureForce snesor 2 is connected with micro- jet mould block 3.The inner chamber phase of the volume displaced pump 1 of Miniature precision, pressure sensor 2 and micro- jet mould block 3It is logical, form path；The volume displaced pump 1 of Miniature precision is used to draw liquid, discharge raffinate and micro liquid is generated in the pathBody pressure required when distributing；Pressure sensor 2 is used to monitor the pressure in the path and feed back to control circuit, by controllingCircuit 4 controls the volume displaced pump 1 of Miniature precision to adjust the pressure in the path.
The volume displaced pump 1 of Miniature precision is that have two outlets on Small Plunger Pump, plunger pump；It can also be Miniature injection pumpWith the combination of a three-way connection, a mouth of three-way connection is connected with Miniature injection pump.
The minute quantity liquid precise distribution instrument of the present embodiment, can all be fixed on mechanism system and realize up and down motionZ axis sliding block on, so as to shorten length of pipe, reduce the accessible volume.
Obviously, different embodiments can be had based on the present invention, or embodiment is extended.In such as Fig. 1, micro- sprayModule 3 can be replaced with a multiple-pass joint for being connected with multiple micro- jet mould blocks, to distribute several samples simultaneously.