A kind of bacterium automatic vaccination instrumentTechnical field
The present invention relates to Micro biological Tests instrument and equipment fields, specifically say a kind of bacterium automatic vaccination instrument.
Background technique
At present micro-biological samples processing be mostly manual operation inoculation, in the prior art, to individual operation level requirement compared withHeight does not merely have to accomplish sterile working, and to be practiced by strict training and long period of operation, could obtain ideal inoculation effectFruit secondly, Inoculation Method overall efficiency is low, and is influenced, part operator by the personal level of operator and inoculation habitSituations such as there may be delay or slow movements, cause to dip bacterial exposure aerial time on oese it is longer andBacteria Culture effect is influenced, the microbionation method working efficiency of this classics is low, it is difficult to carry out accuracy and implementation quality controlSystem is unable to satisfy requirement of the laboratory of modernization to automation, accuracy.
In consideration of it, using micro-biological samples handling machine people or the alternative manual operation of micro-biological samples process instrumentation, it is realExisting system for automatic marker making inoculation, i.e., a kind of bacterium automatic vaccination instrument that can be realized plane method of scoring, specific effect are as follows:
1. a kind of bacterium automatic vaccination instrument of the present invention, instead of being manually inoculated with during microbionationOperation, and can be realized the automatic sterilizing to inoculation environment and microbionation ring, and take bacterium and take lid while carrying out, it shortensThe time of entire seeded process improves working efficiency when microbionation and scribing line.
2. a kind of bacterium automatic vaccination instrument of the present invention, the design of shock module, so that bacterium of the invention is moltenLiquid will not precipitate in test tube, effectively ensure in test tube that bacterial solution is evenly distributed the effect precisely sampled with classification inoculation apparatusFruit.
3. a kind of bacterium automatic vaccination instrument of the present invention, the design of infrared distance sensor and test point, so thatAngles and positions accuracy of the present invention in microbionation, scribing line, accuracy when having reached microbionation, scribing line is high and connectsThe Utopian effect of kind.
Summary of the invention
In order to make up for the deficiencies of the prior art, the present invention provides a kind of bacterium automatic vaccination instrument, can train in bacteriumTraditional artificial plate streaking is replaced with automated procedures during supporting, and avoids the working efficiency of microbionation in the prior artPosition inaccuracy when low, Multiple drug resistance is uneven and inoculation, scribing line, realizes the Utopian function of effect of inoculation.
The technical solution adopted by the present invention to solve the technical problems is: a kind of bacterium automatic vaccination instrument, including body, examinationPipe storing unit, culture dish transmission device, classification inoculation apparatus, bactericidal unit, clamping device and take lid arrangement；The test tube storageDevice is mounted on After-Body and fills germy test tube for storing；The culture dish transmission device is mounted on test tube storageIn front of device and for transporting culture dish；The classification inoculation apparatus is mounted on test tube storing unit right, and classification inoculation apparatus is for dipping inThe bacterium in test tube storing unit is taken, and bacterium is transported to above culture dish transmission device, i.e., scribing position, classification inoculation apparatus are usedIn to culture dish progress plate streaking；The bactericidal unit is mounted on body and is located at right above culture dish transmission device,For being played during microbionation to ambient enviroment and to the sterilization functions of classification inoculation apparatus, bactericidal unit includes bactericidal unitNo.1 sterilization unit for sterilizing to microbionation ring, No. two sterilization units, positions positioned at No.1 sterilization unit rightNo. two test points in front of No.1 sterilization unit and No. three test points right above the culture dish that will be crossed, No.1 are gone outIt is mounted on the flame spray head for sterilizing on bacterium unit and No. two sterilization units, when work, microbionation ring is in classification inoculation apparatusDrive under, move at bactericidal unit, when No. two test points detect microbionation ring reach bactericidal unit at when, inoculation dressStop motion is set, subsequent flame spray head carries out disinfecting action to microbionation ring, eliminates miscellaneous bacteria on microbionation ring to reachPurpose；The clamping device is mounted on body, and for clamping culture dish, described takes lid arrangement to be mounted on body simultaneouslyFor carrying out taking lid to culture dish, the clamping device is mounted on body, and for clamping culture dish.
It is evenly arranged with the pore of depositing for storing test tube on the test tube storing unit, respectively deposits and is equipped with rubber in poreRubber mat, equipped with rubber pad be in order to prevent because caused by the reasons such as vibration, activity test tube damage, while rubber pad is because having centainlyElasticity, be suitable for the test tube of different-diameter, improve the scope of application, test tube is placed in the rubber pad for depositing pore, in test tubeFor storing bacterial solution, and the No.1 test point for being mounted at pore and whether arriving for detecting classification inoculation apparatus is respectively deposited, oneNumber test point purpose of design be in order in seeded process to the accurate positionin of classification inoculation apparatus；In the middle part of the test tube storing unitIt is also equipped with shock module, on the one hand shock module can prevent bacterial solution from precipitating, on the other hand favorably for generating micro-vibrationIn the accurate sampling of classification inoculation apparatus.
The culture dish transmission device includes culture dish, conveyer belt and labelling module, and the culture dish includes wareSeat, ware lid and the culture medium being placed in ware seat, culture dish are uniformly placed on a moving belt；The conveyer belt is mounted on bodyGo up and be used to for culture dish being transported to the underface of classification inoculation apparatus scribing position；The labelling module is mounted on body simultaneouslyFor labelling to culture medium, label is used to record the essential information of the culture medium after inoculation, when such as bacterial species, inoculationBetween etc. information.When work, when needing to carry out plate streaking, then successively the culture dish equipped with culture medium is placed by artificial firstOn a moving belt, then conveyer belt drives culture dish to turn left Uniform Movement to crossing immediately ahead of classification inoculation apparatus from the right side, crossesAfter finishing, the culture dish for being inoculated with completion is transported at labelling module labels again by conveyer belt.
The classification inoculation apparatus includes inoculation rotating disk, inoculation bar, spinner handle motor, is inoculated with transverse slat, takes bacterium electric pushrod, oneNumber infrared ray range sensor takes bacterium module and No. two infrared distance sensors, and the inoculation rotating disk is mounted on body,Inoculation bar is automatic telescopic link and is mounted in inoculation rotating disk by shaft coupling that the spinner handle motor is mounted on inoculation barEnd；The inoculation transverse slat level is mounted on spinner handle motor upper end by shaft coupling, and transverse slat front end is vertically-mounted takes bacterium for inoculationElectric pushrod, the No.1 infrared ray range sensor are mounted on inoculation transverse slat, and No.1 infrared sensor is for detecting oneNumber test point, in classification inoculation apparatus sampling convenient for the accurate positionin of classification inoculation apparatus；It is described to take bacterium module to be mounted on to take bacterium electronicPush rod lower end, taking bacterium module includes fixed block, lifting module, microbionation ring and No. three infrared distances, the fixed block welderingIt is connected in and takes on bacterium electric pushrod, the microbionation ring includes three microbionation rings being sequentially arranged from left to right, is located atFor the microbionation ring of the leftmost side for dipping bacterial solution, other two microbionation rings are used for plate streaking, and described No. threeInfrared distance sensor is mounted on fixed block, and No. three infrared distance sensors are for detection bacterium oese apart from bacterial solutionHeight, avoid microbionation ring not in contact with to bacterial solution or to protrude into bacterial solution too long；No. two infrared sensingsDevice is mounted on inoculation transverse slat, and No. two infrared distance sensors are convenient for for detecting No. two test points in bactericidal unit sterilizingThe accurate positionin of bactericidal unit.When work, classification inoculation apparatus, which is located above bactericidal unit, sterilizes to microbionation ring, has sterilizedCheng Hou, bactericidal unit extinguish, and inoculation rotating disk rotates clockwise, and during rotation, No.1 infrared distance sensor detectsNo.1 test point, microbionation ring is located at right above test tube at this time, is then inoculated with bar decline and shortens oese and bacterial solutionThen distance takes the decline of bacterium electric pushrod to dip bacterial solution, bacterium electric pushrod is then taken to retract, inoculation bar rise makesMicrobionation ring leaves test tube, is then inoculated with rotating disk and rotates counterclockwise, No. two infrared distance sensors detect No. three detectionsPoint, microbionation ring is located at right above culture dish at this time, and inoculation rotating disk stops operating, inoculation bar decline, while bactericidal unitIt lights and sterilizes to ambient enviroment, under the cooperation of clamping device, the common first time scribing line for completing microbionation ring, firstAfter the completion of secondary scribing line, inoculation bar is risen, and spinner handle motor rotates clockwise, and No. two infrared distance sensors detect No. two detectionsPoint, microbionation ring is located at right above bactericidal unit at this time, and two microbionation rings positioned at right stretch out thin with the leftmost sideBacterium oese is consistent, and bactericidal unit is carried out while being sterilized to three microbionation rings at this time, after the completion of sterilizing, spinner handle motorIt rotates counterclockwise and passes through No. two infrared sensors and return to second of scribing line of scribing position progress, three microbionation rings are clampingIt crosses simultaneously under the cooperation of device, then repeats above-mentioned movement and carry out third time scribing line, terminate repetitive operation.It is connect in above-mentioned bacteriumDuring kind ring dips bacterial solution, the shock module moment generates vibration, and the vibration that shock module generates can make bacterial solutionSurface generates ripple, and corrugated surface advantageously forms diffusing reflection, is conducive to the accurate detection of No. three infrared distance sensors.
The clamping device is located at scribing position and is used for the clamping of culture dish, clamping device include after folder unit andFore-clamp unit, the rear folder unit and fore-clamp unit are installed on body and are located at culture dish transmission device front and rear sides；Folder unit includes grip slipper, two sliding blocks, spring and upper driven wheel afterwards, is provided in the middle part of grip slipper for sliding block reciprocating motionSliding slot, each sliding block are slidably mounted in sliding slot, and spring is connected between each sliding block and grip slipper, and each slip front passes throughA piece connecting rod is connected with driven wheel, and upper driven wheel is in contact with the ware seat of culture dish, the purpose of the extensional motion design of springIt is in order to keep the spacing between two upper driven wheels adjustable, to adapt to the diameter of culture dish；The fore-clamp unit includes folderIt holds push rod, gripper jaw, lower driving wheel, lower driven wheel and clamping motor, the clamping push rod to be mounted on body, clamps push rodEnd is fixed with gripper jaw, and gripper jaw inner sidewall is equipped with lower driving wheel and lower driven wheel for touching with culture dish ware seated connection,The clamping motor is mounted on gripper jaw and connects lower driving wheel.When work, clamping push rod, which will stretch out a certain distance, to be trainedFeeding ware pushes upper driven wheel to, and culture dish is clamped under the collective effect of lower driving wheel, lower driven wheel and upper driven wheel, in plateWhen scribing line, microbionation ring contact culture medium is motionless, and then gripper jaw drives lower driving wheel clockwise movement, at this time culture dish meetingAnd then it rotating, microbionation ring is crossed, and is crossed after certain distance, and lower driving wheel stops operating, and microbionation ring rises, underDriving wheel rotates counterclockwise makes culture dish be returned to initial position, and clamping push rod retracts a certain distance backward, to carry out secondIt the scribing line of section and then repeats the above steps and carries out third time scribing line, three sections of lines complete first time scribing line after having drawn, then bacteriumOese rises and reaches sterilization station and sterilizes, while clamping push rod stretching makes culture dish be returned to initial position, thenClamping device cooperates three microbionation rings to be crossed simultaneously to complete second of scribing line, sterilizes after the completion of second of scribing line,It repeats second of movement and carries out third time scribing line, until scribing line is completed.
The lid arrangement that takes includes taking lid rotating disk, taking lid bar, take lid motor, take cover board and take lid terminal, describedLid rotating disk is taken to be mounted on body, described to take lid bar to be mounted on to take in lid rotating disk, taking lid bar is automatic telescopic rod piece, is takenLid bar is automatic telescopic rod piece, takes lid bar upper end to be equipped with and takes lid motor, and described to take lid motor for servo motor, described takes lidPlate, which is horizontally arranged at, to be taken on lid motor, is taken cover board lower end to be equipped with and is taken lid terminal；The lid terminal that takes includes two horizontal reversesTo be arranged in take cover board lower end surface take lid push rod, grip block, elastomeric pad and pressure sensor, the grip block is connected inLid push rod front end is respectively taken, the elastomeric pad is mounted on the inside of grip block and for being in contact with ware lid, elastomeric pad and folderHold and pressure sensor be installed between plate, the described elastomeric pad heatproof is high, the purpose of elastomeric pad design be in order to avoid byIt is excessively high in the temperature of ware lid and cause to damage pressure sensor.When work, takes cover board to be located at right above culture dish, take lidPlate decline takes ware lid between lid terminal positioned at two, and lid push rod retraction is then taken to clamp ware lid, and then taking lid bar to rise willWare lid is removed from ware seat, and after ware lid reaches certain altitude, taking lid motor to rotate counterclockwise certain angle makes ware lid be located at sterilizingIt sterilizes above device to ware lid, inoculation dashing work can be carried out at this time, after the completion of inoculation, lid motor is taken to turn clockwiseIt is dynamic, be located at ware lid immediately below ware seat, lid bar then taken to rise, be next time take lid to prepare.
The beneficial effects of the present invention are:
1. a kind of bacterium automatic vaccination instrument of the present invention, instead of being manually inoculated with during microbionationOperation, and can be realized the automatic sterilizing to inoculation environment and microbionation ring, and take bacterium and take lid while carrying out, it shortensThe time of entire seeded process, working efficiency when improving microbionation and scribing line.
2. a kind of bacterium automatic vaccination instrument of the present invention, the design of shock module, so that bacterium of the invention is moltenLiquid will not precipitate in test tube, effectively ensure in test tube that bacterial solution is evenly distributed the effect precisely sampled with classification inoculation apparatusFruit.
3. a kind of bacterium automatic vaccination instrument of the present invention, the design of infrared distance sensor test point, so that thisAngles and positions accuracy of the invention in microbionation, scribing line, accuracy when having reached microbionation, scribing line is high and is inoculated withUtopian effect.
Detailed description of the invention
The present invention is further described with embodiment with reference to the accompanying drawing.
Fig. 1 is schematic top plan view of the invention；
Structural schematic diagram when Fig. 2 is present invention scribing line；
Fig. 3 is the structural schematic diagram of classification inoculation apparatus of the present invention；
Fig. 4 is the structural schematic diagram that the present invention takes lid arrangement；
Fig. 5 is the partial structure diagram of Fig. 4；
In figure: including body 1, test tube storing unit 2, culture dish transmission device 3, classification inoculation apparatus 4, bactericidal unit 5, clampingDevice 6, take lid arrangement 7, deposit pore 21, rubber pad 22, No.1 test point 23, shock module 24, culture dish 31, conveyer belt 32,Labelling module 33, ware seat 311, ware lid 312, culture medium 313, inoculation rotating disk 41, inoculation bar 42, spinner handle motor 43, inoculationTransverse slat 44 takes bacterium electric pushrod 45, No.1 infrared distance sensor 46, takes 47, No. two infrared distance sensors 48 of bacterium module, is solidDetermine block 471, lifting module 472,473, No. three infrared distance sensors 474 of microbionation ring, No.1 sterilization unit 51, two go outThe test point 53, three of bacterium unit 52, two test point 54, rear folder unit 61, fore-clamp unit 62, grip slipper 611, sliding block 612, bulletSpring 613, upper driven wheel 614, clamping push rod 621, gripper jaw 622, lower driving wheel 623, lower driven wheel 624, clamping motor 625,Lid rotating disk 71 is taken, lid bar 72 is taken, takes lid motor 73, take cover board 74, take lid terminal 75, take lid push rod 741, grip block 742, bulletProperty gasket 743, pressure sensor 744.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie belowSpecific embodiment is closed, the present invention is further explained.
As shown in Figures 1 to 5, a kind of bacterium automatic vaccination instrument of the present invention, including body 1, test tube storing unit2, culture dish transmission device 3, classification inoculation apparatus 4, bactericidal unit 5, clamping device 6 and lid arrangement 7 is taken；The test tube storing unit2 are mounted on 1 rear portion of body and fill germy test tube for storing；The culture dish transmission device 3 is mounted on test tube storage2 front of device simultaneously is used to transport culture dish；The classification inoculation apparatus 4 is mounted on 2 right of test tube storing unit, and classification inoculation apparatus 4 is usedIn dipping the bacterium in test tube storing unit 2, and bacterium is transported to 3 top of culture dish transmission device, i.e. scribing position, inoculationDevice 4 is used to carry out plate streaking to culture dish；The bactericidal unit 5 is mounted on body 1 and is located at culture dish transmission dressSet right above 3, bactericidal unit 5 for being played during microbionation to ambient enviroment and to the sterilization functions of classification inoculation apparatus 4,Bactericidal unit 5 includes No.1 sterilization unit 51 for sterilizing to microbionation ring, is located at 51 right side of No.1 sterilization unitNo. two sterilization units 52, No. two test points 53 positioned at the front of No.1 sterilization unit 51 and No. three detections positioned at scribing position54 are put, the flame spray head for sterilizing is mounted on No.1 sterilization unit 51 and No. two sterilization units 52；The clamping dressIt sets 6 to be mounted on body 1, and for clamping culture dish, described takes lid arrangement 7 to be mounted on body 1 and for culture dishIt carries out taking lid, the clamping device 6 is mounted on body 1, and for clamping culture dish.
As shown in Figure 1, Figure 2 and Figure 3, a kind of bacterium automatic vaccination instrument of the present invention, the test tube storing unit 2On be evenly arranged with and deposit pore 21 for store test tube, respectively deposit and be equipped with rubber pad 22 in pore 21, be equipped with rubber pad 22In order to prevent because caused by the reasons such as vibration, activity test tube damage, while rubber pad 22 is because being suitable for not with certain elasticityWith the test tube of diameter, the scope of application is improved, test tube is placed in the rubber pad 22 for depositing pore 21, for storing bacterium in test tubeSolution, and respectively deposit and be mounted on No.1 test point 23 at pore 21,23 purpose of design of No.1 test point is easy in seeded processThe accurate positionin of middle classification inoculation apparatus 4；Shock module 24 is also equipped in the middle part of the test tube storing unit 2, shock module 24 is usedIn generating micro-vibration, it on the one hand can prevent bacterial solution from precipitating, on the other hand be conducive to the accurate sampling of classification inoculation apparatus 4.
As shown in Figure 1, Figure 2 and Figure 3, a kind of bacterium automatic vaccination instrument of the present invention, the culture dish transmit dressSetting 3 includes culture dish 31, conveyer belt 32 and labelling module 33, and the culture dish 31 includes ware seat 311, ware lid 312 and putsThe culture medium 313 in ware seat 311 is set, culture dish 31 is uniformly placed on conveyer belt 32；The conveyer belt 32 is mounted on machineUnderface on body 1 and for culture dish 31 to be transported to 4 scribing position of classification inoculation apparatus；The labelling module 33 is mounted onOn body 1 and for labelling to culture medium 313, label is used to record the essential information of the culture medium 313 after inoculation, such asThe information such as bacterial species, inoculation time.When work, when needing to carry out plate streaking, then pass through artificial or mechanical equipment firstSuccessively culture dish 31 is placed on conveyer belt 32, then conveyer belt 32 drives culture dish 31 to turn left Uniform Movement to inoculation from the right sideIt crosses immediately ahead of device 4, after scribing line finishes, the culture dish 31 for being inoculated with completion is transported to labelling again by conveyer belt 32It labels at module 33.
Such as Fig. 1 and with shown in 2, a kind of bacterium automatic vaccination instrument of the present invention, the classification inoculation apparatus 4 includes inoculationRotating disk 41, spinner handle motor 43, inoculation transverse slat 44, takes bacterium electric pushrod 45, No.1 infrared ray range sensor at inoculation bar 4246, bacterium module 47 and No. two infrared distance sensors 48 are taken, the inoculation rotating disk 41 is mounted on body 1, is inoculated with bar 42It is mounted in inoculation rotating disk 41, the spinner handle motor 43 is mounted on inoculation 42 upper end of bar；The inoculation transverse slat 44 is horizontalBe mounted on spinner handle motor 43, inoculation 44 front end of transverse slat is vertically-mounted to take bacterium electric pushrod 45, the No.1 infrared ray away fromIt is mounted on inoculation transverse slat 44 from sensor 46, No.1 infrared sensor 46 is for detecting No.1 test point 23, in classification inoculation apparatus 4Convenient for the accurate positionin of classification inoculation apparatus 4 when sampling；It is described to take bacterium module 47 to be mounted on to take 45 lower end of bacterium electric pushrod, take bacterium mouldBlock 47 includes fixed block 471, lifting module 472, microbionation ring 473 and No. three infrared distance 474, the fixed block 471It is connected in and takes on bacterium electric pushrod 45, the microbionation ring 473 includes three microbionations being sequentially arranged from left to rightRing 474, for the microbionation ring positioned at the leftmost side for dipping bacterial solution, other two microbionation rings are used for plate streaking,No. three infrared distance sensors 474 are mounted on fixed block 471, and No. three infrared distance sensors 474 are thin for detectingHeight of the bacterium oese 473 apart from bacterial solution avoids microbionation ring 473 not in contact with to bacterial solution or to protrude into bacterium moltenLiquid is too long；No. two infrared sensors 48 are mounted on inoculation transverse slat 44, and No. two infrared distance sensors 48 are for detectingNo. two test points 53 and No. three test points 54, when bactericidal unit 5 sterilizes convenient for the accurate positionin of classification inoculation apparatus 4.When work, connectKind device 4 is located at 5 top of bactericidal unit and sterilizes to microbionation ring 473, and after the completion of sterilizing, bactericidal unit 5 extinguishes, inoculationRotating disk 41 rotates clockwise, and during rotation, No.1 infrared distance sensor 46 detects No.1 test point 23, at this time carefullyBacterium oese 473 is located at right above test tube, is then inoculated with the decline of bar 42 and shortens oese 473 at a distance from bacterial solution, thenIt takes the decline of bacterium electric pushrod 45 to dip bacterial solution, bacterium electric pushrod 45 is then taken to retract, the inoculation rise of bar 42 makes thinBacterium oese 473 leaves test tube, is then inoculated with rotating disk 41 and rotates counterclockwise, No. two infrared distance sensors 48 detect No. threeTest point 54, microbionation ring 473 is located at right above culture dish at this time, and inoculation rotating disk 41 stops operating, and inoculation bar 42 declines,Bactericidal unit 5, which is lighted, simultaneously sterilizes to ambient enviroment, common to complete microbionation ring 473 under the cooperation of clamping device 6First time scribing line, after the completion of crossing for the first time, inoculation bar 42 is risen, and spinner handle motor 43 rotates clockwise, No. two infrared distancesSensor 48 detects No. two test points 53, and microbionation ring 473 is located at right above bactericidal unit 5 at this time, positioned at the two of rightA microbionation ring 473 is stretched out to be consistent with the microbionation ring 473 of the leftmost side, and bactericidal unit 5 connects three bacteriums at this timeKind ring 473 is carried out while being sterilized, and after the completion of sterilizing, spinner handle motor 43 is rotated counterclockwise and returned to by No. two infrared sensors 48Scribing position carries out second and crosses, and three microbionation rings 473 are crossed simultaneously under the cooperation of clamping device 6, then repeatedAbove-mentioned movement carries out third time scribing line, repeats above-mentioned movement until scribing line terminates.Bacterial solution is dipped in above-mentioned microbionation ringDuring, the shock module moment generates vibration, and the vibration that shock module generates can make bacterial solution surface generate ripple, rippleFace advantageously forms diffusing reflection, is conducive to accurate detection of No. three infrared distance sensors 474 to bacterial solution.
As shown in Figure 1, Figure 2 and Figure 4, a kind of bacterium automatic vaccination instrument of the present invention, the clamping device 6 are located atClamping at scribing position and for culture dish 31, clamping device 6 press from both sides unit 61 and fore-clamp unit 62, the rear folder after includingUnit 61 and fore-clamp unit 62 are installed on body 1 and are located at 3 front and rear sides of culture dish transmission device；Folder unit 61 includes afterwards611, two sliding blocks 612 of grip slipper, spring 613 and upper driven wheel 614 are provided in the middle part of grip slipper 611 reciprocal for sliding block 612The sliding slot of movement, each sliding block 612 are slidably mounted in sliding slot, are connected with spring between each sliding block 612 and grip slipper 611613, each 612 front end of sliding block passes through a connecting rod and is connected with driven wheel 614, the ware seat of upper driven wheel 614 and culture dish 31311 are in contact, the purpose of the extensional motion of spring 613 design be in order to keep the spacing between two upper driven wheels 614 adjustable,To adapt to the diameter of culture dish 31；The fore-clamp unit 62 include clamping push rod 621, gripper jaw 622, lower driving wheel 623, underDriven wheel 624 and clamping motor 625, the clamping push rod 621 are mounted on body 1, and clamping 621 end of push rod is fixed with folderPawl 622 is held, 622 inner sidewall of gripper jaw is equipped with lower driving wheel 623 and lower driven wheel for contacting with 31 ware seat 311 of culture dish624, the clamping motor 625 is mounted on gripper jaw 622 and connects lower driving wheel 623.When work, clamping push rod 621 is stretchedA certain distance pushes culture dish 31 to upper driven wheel 614 out, culture dish 31 lower driving wheel 623, lower driven wheel 624 and on fromIt is clamped under the collective effect of driving wheel 614, in plate streaking, the contact culture medium 313 of microbionation ring 473 is motionless, then presss from both sidesIt holds pawl 622 and drives lower 623 clockwise movement of driving wheel, culture dish 31 can be rotated and then at this time, and microbionation ring 473 is drawnLine is crossed after certain distance, and lower driving wheel 623 stops operating, and microbionation ring 473 rises, the lower rotation counterclockwise of driving wheel 623Culture dish 31 is set to be returned to initial position, clamping push rod 621 retracts a certain distance backward, to carry out the scribing line of second segment thenIt repeats the above steps and carries out third time scribing line, three sections of lines are completed to cross for the first time after having drawn, and then microbionation ring 473 risesAnd reach sterilization station and sterilize, while clamping the stretching of push rod 621 makes culture dish 31 be returned to initial position, then clamping fills6 cooperation, three microbionation rings 473 while scribing line are set to complete second of scribing line, are sterilized after the completion of second of scribing line, weightMultiple second of movement carries out third time scribing line, until scribing line is completed.
As shown in Figure 1, Figure 2, shown in Fig. 4 and Fig. 5, a kind of bacterium automatic vaccination instrument of the present invention, described takes lid arrangement 7Including taking lid rotating disk 71, taking lid bar 72, take lid motor 73, take cover board 74 and take lid terminal 75, described takes lid rotating disk 71It is mounted on body 1, described to take lid bar 72 to be mounted on to take in lid rotating disk 71, taking lid bar 72 is automatic telescopic rod piece, takes lid72 upper end of bar, which is equipped with, takes lid motor 73, and described to take lid motor 73 be servo motor, described to take cover board 74 to be horizontally arranged to takeOn lid motor 73, takes 74 lower end of cover board to be equipped with and take lid terminal 75；The lid terminal 75 that takes includes two horizontal reversed arrangementsLid push rod 741, grip block 742, elastomeric pad 743 and pressure sensor 744, the clamping are taken take 74 lower end surface of cover boardPlate 742 is connected in and respectively takes 741 front end of lid push rod, and the elastomeric pad 743 is mounted on the inside of grip block 742 and is used for and ware lid312 are in contact, and pressure sensor 744,743 heatproof of elastomeric pad are equipped between elastomeric pad 743 and grip block 742Height, the purpose that elastomeric pad 743 designs is to cause in order to avoid the temperature due to ware lid 312 is excessively high to pressure sensor 744It damages.When work, cover board 74 is taken to be located at right above culture dish 31, takes the decline of cover board 74 that ware lid 312 is made to be located at two and take lidBetween terminal 75, then takes the retraction of lid push rod 741 to clamp ware lid 312, lid bar 72 is then taken to rise ware lid 312 from ware seat311 remove, and after ware lid 312 reaches certain altitude, taking lid motor 73 to rotate counterclockwise certain angle makes ware lid 312 be located at sterilizing5 top of device sterilizes to ware lid 312, can carry out inoculation dashing work at this time, after the completion of inoculation, take 73 up time of lid motorNeedle rotation, is located at ware lid 312 immediately below ware seat 311, and lid bar 72 is then taken to rise, be next time take lid to prepare.
When work, stored firstly, the test tube equipped with bacterial solution is sequentially placed into test tube by artificial or machinery equipmentIn device 2, then, then culture dish transmission device 3 will be sequentially placed into equipped with the culture dish 31 of culture medium 313；It is thin when needing to carry outWhen bacterium is inoculated with, classification inoculation apparatus 4 is located at 5 top of bactericidal unit and sterilizes to microbionation ring 473, after the completion of sterilizing, sterilizing dress5 extinguishings are set, inoculation rotating disk 41 rotates clockwise, and during rotation, No.1 infrared distance sensor 46 detects that No.1 is examinedMeasuring point 23, microbionation ring 473 is located at right above test tube at this time, is then inoculated with the decline of bar 42 shortening oese 473 and bacterium is moltenThen the distance of liquid takes the decline of bacterium electric pushrod 45 to dip bacterial solution, bacterium electric pushrod 45 is then taken to retract, inoculationThe rise of bar 42 makes microbionation ring 473 leave test tube, is then inoculated with rotating disk 41 and rotates counterclockwise, No. two infrared distance sensors48 detect No. three test points 54, and microbionation ring 473 is located at right above culture dish at this time, and inoculation rotating disk 41 stops operating,It is inoculated with bar 42 to decline, while bactericidal unit 5 is lighted and carries out disinfection inoculation device 4 to ambient enviroment and take lid arrangement 7 while carrying out,It takes cover board 74 to be located at right above culture dish 31, takes the decline of cover board 74 to make 611 to be located at two and take between lid terminal 75, then lid is taken to push awayThe retraction of bar 741 clamps grip slipper 611, and the rise of lid bar 72 is then taken to remove ware lid 312 from ware seat 311, when ware lid 312 reachesAfter certain altitude, take lid motor 73 rotate counterclockwise certain angle make ware lid 312 be located at the top of bactericidal unit 5 to ware lid 312 intoRow sterilizing can carry out the first time scribing line of microbionation ring 473 at this time, and after the completion of crossing for the first time, inoculation bar 42 is risen, rotationBar motor 43 rotates clockwise, and No. two infrared distance sensors 48 detect No. two test points 53, at this time 473, microbionation ringRight above bactericidal unit 5, two microbionation rings 473 positioned at right are stretched out to be kept with the microbionation ring 473 of the leftmost sideUnanimously, bactericidal unit 5 is carried out while being sterilized to three microbionation rings 473 at this time, and after the completion of sterilizing, spinner handle motor 43 is counterclockwiseIt rotates and passes through No. two infrared sensors 48 and return to second of scribing line of scribing position progress, three microbionation rings 473 are clampingIt crosses simultaneously under the cooperation of device 6, then repeats above-mentioned movement and carry out third time scribing line, until scribing line is completed, terminate to repeat dynamicMake, after the completion of inoculation, lid motor 73 is taken to rotate clockwise, is located at grip slipper 611 immediately below ware seat 311, then clamps push rod621 stretching a certain distance push culture dish 31 to upper driven wheel 614, and culture dish 31 is in lower driving wheel 623, lower driven wheel 624It is clamped under the collective effect of upper driven wheel 614, in plate streaking, the contact culture medium 313 of microbionation ring 473 is motionless,Then gripper jaw 622 drives lower 623 clockwise movement of driving wheel, and culture dish 31 can be rotated and then at this time, microbionation ring 473 intoRow scribing line, crosses after certain distance, and lower driving wheel 623 stops operating, and microbionation ring 473 rises, and lower driving wheel 623 is counterclockwiseRotation makes culture dish 31 be returned to initial position, and clamping push rod 621 retracts a certain distance backward, to carry out the scribing line of second segmentThen it repeats the above steps and carries out third time scribing line, three sections of lines are completed to cross for the first time after having drawn, then microbionation ring 473It rises and reaches sterilization station and sterilize, while clamping the stretching of push rod 621 makes culture dish 31 be returned to initial position, then presss from both sidesIt holds device 6 to cooperate three microbionation rings 473 while crossing to complete second of scribing line, go out after the completion of second of scribing lineBacterium repeats second of movement and carries out third time scribing line, until scribing line is completed, after scribing line finishes, by ware lid 312 in clamping deviceCooperation under be placed on ware seat 311, then the culture dish 31 for being inoculated with completion is transported to labelling module again by conveyer belt 32It labels at 33, and then completes entire plane system for automatic marker making process.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry shouldUnderstand, the present invention is not limited to the above embodiments, and only illustrating for the description in above embodiments and description is of the inventionPrinciple, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these change and changeInto all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalentIt defines.