技术领域technical field
本发明属于光学显微立体成像技术领域，特别是一种偏振式动态实时体视显微成像装置及方法。The invention belongs to the technical field of optical microscopic stereoscopic imaging, in particular to a polarized dynamic real-time stereoscopic microscopic imaging device and method.
背景介绍background introduction
人眼具有立体视觉的效果，可以区分物体的前后、远近，这是由于人类感知自然深度是由于左、右眼观看真实世界有轻微的差异造成的。立体视觉来源于“视差”，人的双眼有4—6cm的距离，观看物体时，左眼看到物体左侧面的成分较多，右眼看到物体右侧面的成分较多，所以实际上我们看到物体时两只眼睛中的图像是有差别的，这个差别被称为“视差”。大脑具有解读视差的能力，这两个有差异的图像经过大脑综合以后就能区分物体的深度层次关系，从而产生立体视觉，我们看到的是有景深的图像，这就是人眼立体视觉的原理。一般来说，图像的视差值越大，立体感越强，即在表现同一物体时，两台相机模拟人眼距离越大，立体感越强。但距离不可无限增大，因为当距离无限增大时，不再满足获得立体视觉效果的条件，不可为双眼提供具有一定深度信息的视差，从而不能形成立体视觉。传统立体显微镜由一个共用的初级物镜对物体成像后，经过两组倍率可以调节的中间物镜组分开，然后经过角度12-15°的目镜组成像，这样的话便可以为左右眼分别提供角度不同的图像，经过大脑的综合处理后，人眼看到的就是一幅有景深的立体视觉图像。The human eye has the effect of stereoscopic vision, and can distinguish the front, back, and distance of objects. This is because the natural depth of human perception is caused by the slight difference in viewing the real world with the left and right eyes. Stereoscopic vision comes from "parallax". There is a distance of 4-6cm between human eyes. When viewing an object, the left eye sees more components on the left side of the object, and the right eye sees more components on the right side of the object. So in fact we There is a difference between the images in the two eyes when seeing an object, and this difference is called "parallax". The brain has the ability to interpret parallax. After the two different images are synthesized by the brain, the depth and hierarchical relationship of objects can be distinguished, thereby producing stereo vision. What we see is an image with depth of field. This is the principle of human stereo vision . Generally speaking, the larger the parallax value of the image, the stronger the stereoscopic effect, that is, when the same object is represented, the greater the distance between the simulated human eyes of the two cameras, the stronger the stereoscopic effect. However, the distance cannot be increased infinitely, because when the distance increases infinitely, the conditions for obtaining the stereoscopic effect are no longer met, and the parallax with a certain depth information cannot be provided for the eyes, so that the stereoscopic vision cannot be formed. The traditional stereo microscope uses a common primary objective lens to image the object, and separates it through two sets of intermediate objective lens groups with adjustable magnification, and then forms an image through the eyepieces with an angle of 12-15°, so that the left and right eyes can be provided with different angles. After the image is comprehensively processed by the brain, what the human eye sees is a stereoscopic image with depth of field.
传统的立体显微镜优点很明显：使用方便，操作简便。但是它存在的问题也是无法弥补的：(1)焦深和体视角是不可调的，由于人眼瞳距个体差异，人眼的体视角不一样，这样会导致个人观看效果的不理想；(2)双通道光路不仅使仪器笨重而且成本也会加大；(3)成像视野小，成像质量较差，不可以动态实时观看到物体的详细信息。The advantages of traditional stereo microscopes are obvious: easy to use and easy to operate. But its problems are also irreparable: (1) The depth of focus and the stereoscopic viewing angle are not adjustable. Due to the individual differences in the interpupillary distance of the human eye, the stereoscopic viewing angle of the human eye is different, which will lead to unsatisfactory personal viewing effects; ( 2) The dual-channel optical path not only makes the instrument bulky but also increases the cost; (3) The imaging field of view is small, the imaging quality is poor, and the detailed information of the object cannot be dynamically viewed in real time.
发明内容Contents of the invention
本发明的目的在于提供一种装置简单、颜色效果好、焦深和体视角均可灵活调整的偏振式动态实时体视显微成像装置及方法，实现对样品的动态实时观看，提升观察者体验度。The purpose of the present invention is to provide a polarized dynamic real-time stereomicroscopic imaging device and method with simple device, good color effect, and flexible adjustment of focal depth and volume angle of view, so as to realize dynamic real-time viewing of samples and improve the experience of observers Spend.
实现本发明目的的技术解决方案为：一种偏振式动态实时体视显微成像装置，包括P4系列LED阵列、样品载物台、显微物镜、镜筒透镜、摄像机、偏振式3D显示器、计算机和偏振式3D眼镜；其中P4系列LED阵列、样品载物台、显微物镜、镜筒透镜、摄像机从下至上依次设置，且P4系列LED阵列的中心位于显微物镜、镜筒透镜的中心轴线上；P4系列LED阵列和摄像机的控制端均与计算机连接，摄像机的输出端接入偏振式3D显示器；The technical solution for realizing the object of the present invention is: a polarized dynamic real-time stereomicroscopic imaging device, including a P4 series LED array, a sample stage, a microscopic objective lens, a lens tube lens, a video camera, a polarized 3D display, a computer And polarized 3D glasses; where the P4 series LED array, sample stage, microscope objective lens, lens tube lens, and camera are arranged in sequence from bottom to top, and the center of the P4 series LED array is located on the central axis of the microscope objective lens and lens tube lens Above; the P4 series LED array and the control end of the camera are connected to the computer, and the output end of the camera is connected to the polarized 3D display;
将待成像的样品置于样品载物台，计算机向P4系列LED阵列发送触发信号，使P4系列LED阵列交替产生两个圆形照明光，照明光透过样品载物台被显微物镜收集，显微物镜将收集的照明光进行放大成像后入射至镜筒透镜，计算机向驱动摄像机发送触发信号以驱动摄像机对穿过镜筒透镜的样品图像进行采样，摄像机将采集的样品图像输入偏振式3D显示器进行显示，观察者通过佩戴偏振式3D眼镜观看偏振式3D显示器即可实时观看到样品图像的立体显微结构。The sample to be imaged is placed on the sample stage, and the computer sends a trigger signal to the P4 series LED array, so that the P4 series LED array alternately generates two circular illumination lights, and the illumination light passes through the sample stage and is collected by the microscope objective lens. The microscope objective lens magnifies the collected illumination light and then enters the lens tube lens. The computer sends a trigger signal to the driving camera to drive the camera to sample the sample image passing through the lens tube lens. The camera inputs the collected sample image into the polarized 3D The display is displayed, and the observer can observe the stereoscopic microstructure of the sample image in real time by wearing the polarized 3D glasses to watch the polarized 3D display.
一种基于所述装置的偏振式动态实时体视显微成像方法，步骤如下：A polarization dynamic real-time stereomicroscopic imaging method based on the device, the steps are as follows:
步骤1，将待成像的样品置于样品载物台，计算机向P4系列LED阵列发送触发信号，使P4系列LED阵列交替产生两个圆形照明光；Step 1. Place the sample to be imaged on the sample stage, and the computer sends a trigger signal to the P4 series LED array, so that the P4 series LED array alternately generates two circular illumination lights;
步骤2，步骤1产生的照明光透过样品载物台被显微物镜收集，显微物镜将收集的照明光进行放大成像后入射至镜筒透镜；Step 2, the illumination light generated in step 1 passes through the sample stage and is collected by the microscope objective lens, and the microscope objective lens magnifies the collected illumination light and then enters the tube lens;
步骤3，计算机向驱动摄像机发送触发信号以驱动摄像机对穿过镜筒透镜的样品图像进行采样，得到分别对应于两个圆形照明光的显微样品图像；Step 3, the computer sends a trigger signal to the driving camera to drive the camera to sample the image of the sample passing through the barrel lens, and obtain microscopic sample images respectively corresponding to the two circular illumination lights;
步骤4，摄像机将步骤3拍摄到的两个显微样品图像输入偏振式3D显示器分别作为左、右眼图像进行显示；Step 4, the camera inputs the two microscopic sample images captured in step 3 into the polarized 3D display as left and right eye images for display;
步骤5，观察者通过佩戴偏振式3D眼镜观看偏振式3D显示器即可实时观看到样品图像的立体显微结构。In step 5, the observer can observe the stereoscopic microstructure of the sample image in real time by wearing the polarized 3D glasses and viewing the polarized 3D display.
本发明与现有技术相比，其显著优点在于：(1)P4系列LED阵列的使用，取代了传统立体显微镜的双光路，简化了系统设计，不仅使成像系统简便而且有效降低成本；(2)通过采用LED阵列作为照明光源，具有系统本身的光源，受外界干扰较小，观察条件的限制性降低，在暗背景下能够进行观看；(3)通过控制P4系列LED阵列上的圆形照明光源的半径r和圆心距离d分别实现对焦深和体视角的灵活可调；(4)将摄像机采集到的图像传到偏振式3D显示器上，从而可以实现对样品立体结构的动态实时清晰观看，提升观察者体验度。Compared with the prior art, the present invention has significant advantages in that: (1) the use of the P4 series LED array replaces the dual optical paths of the traditional stereo microscope, simplifies the system design, not only makes the imaging system simple but also effectively reduces the cost; (2) )By using the LED array as the lighting source, it has the light source of the system itself, which is less affected by external interference, and the restriction of the observation conditions is reduced, and it can be viewed under a dark background; (3) By controlling the circular lighting on the P4 series LED array The radius r of the light source and the distance d from the center of the circle respectively realize the flexible adjustment of the depth of focus and the perspective of the volume; (4) the images collected by the camera are transmitted to the polarized 3D display, so that the dynamic real-time and clear viewing of the three-dimensional structure of the sample can be realized. Improve observer experience.
附图说明Description of drawings
图1是本发明偏振式动态实时体视显微成像装置的结构示意图。Fig. 1 is a structural schematic diagram of a polarized dynamic real-time stereo microscope imaging device of the present invention.
图2是本发明中P4系列LED阵列产生的白色圆形照明光示意图，(a)图为初始化时P4系列LED阵列产生的白色圆形照明光示意图示意图，(b)图为某一时刻P4系列LED阵列产生的白色圆形照明光示意图，(c)图为下一时刻P4系列LED阵列产生的白色圆形照明光示意图。Fig. 2 is a schematic diagram of the white circular illumination light produced by the P4 series LED array in the present invention, (a) is a schematic diagram of the white circular illumination light produced by the P4 series LED array during initialization, (b) is a schematic diagram of the white circular illumination light produced by the P4 series LED array at a certain moment The schematic diagram of the white circular illumination light produced by the LED array, (c) is the schematic diagram of the white circular illumination light produced by the P4 series LED array at the next moment.
图3是本发明中P4系列LED阵列控制体视角和焦深的原理示意图。Fig. 3 is a schematic diagram of the principle of the P4 series LED array control volume viewing angle and focal depth in the present invention.
图4是本发明实施例中摄像机所拍摄到的样品图。Fig. 4 is a sample diagram captured by the camera in the embodiment of the present invention.
具体实施方式detailed description
下面结合附图及具体实施例对本发明作进一步详细说明。The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.
结合图1，本发明偏振式动态实时体视显微成像装置，包括P4系列LED阵列1、样品载物台2、显微物镜3、镜筒透镜4、摄像机5、偏振式3D显示器6、计算机7和偏振式3D眼镜8；其中P4系列LED阵列1、样品载物台2、显微物镜3、镜筒透镜4、摄像机5从下至上依次设置，且P4系列LED阵列1的中心位于显微物镜3、镜筒透镜4的中心轴线上；P4系列LED阵列1和摄像机5的控制端均与计算机7连接，摄像机5的输出端接入偏振式3D显示器6；1, the polarized dynamic real-time stereomicroscopic imaging device of the present invention includes a P4 series LED array 1, a sample stage 2, a microscopic objective lens 3, a barrel lens 4, a camera 5, a polarized 3D display 6, and a computer 7 and polarized 3D glasses 8; wherein the P4 series LED array 1, the sample stage 2, the microscope objective lens 3, the lens barrel lens 4, and the camera 5 are arranged in sequence from bottom to top, and the center of the P4 series LED array 1 is located in the microscope On the central axis of the objective lens 3 and the lens barrel lens 4; the control ends of the P4 series LED array 1 and the camera 5 are connected to the computer 7, and the output end of the camera 5 is connected to a polarized 3D display 6;
将待成像的样品置于样品载物台2，计算机7向P4系列LED阵列1发送触发信号，使P4系列LED阵列1交替产生两个圆形照明光，照明光透过样品载物台2被显微物镜3收集，显微物镜3将收集的照明光进行放大成像后入射至镜筒透镜4，计算机7向驱动摄像机5发送触发信号以驱动摄像机5对穿过镜筒透镜4的样品图像进行采样，摄像机5将采集的样品图像输入偏振式3D显示器6进行显示，观察者通过佩戴偏振式3D眼镜8观看偏振式3D显示器6即可实时观看到样品图像的立体显微结构。The sample to be imaged is placed on the sample stage 2, and the computer 7 sends a trigger signal to the P4 series LED array 1, so that the P4 series LED array 1 alternately generates two circular illumination lights, and the illumination light passes through the sample stage 2 and is captured. The microscopic objective lens 3 collects, and the microscopic objective lens 3 magnifies the collected illumination light and then enters the lens tube lens 4, and the computer 7 sends a trigger signal to the drive camera 5 to drive the camera 5 to perform image processing on the sample image passing through the lens tube lens 4. For sampling, the camera 5 inputs the collected sample image into the polarized 3D display 6 for display, and the observer wears the polarized 3D glasses 8 and watches the polarized 3D display 6 to view the stereoscopic microstructure of the sample image in real time.
优选地，所述P4系列LED阵列1提供红色、绿色、蓝色、青色、粉色、黄色、白光七种颜色的照明光，本装置采用任意颜色照明光均可，两个圆形照明光源颜色相同即可；P4系列LED阵列的单元板尺寸为128mm*128mm、像素个数为32*32、像素间距4mm，每个像素点均可单独点亮。Preferably, the P4 series LED array 1 provides seven colors of illumination light: red, green, blue, cyan, pink, yellow, and white. The device can use any color of illumination light, and the two circular illumination sources have the same color That’s it; the size of the unit board of the P4 series LED array is 128mm*128mm, the number of pixels is 32*32, and the pixel pitch is 4mm. Each pixel can be individually lit.
优选地，所述P4系列LED阵列1距离样品载物台2上表面的距离为75～85mm。Preferably, the distance between the P4 series LED array 1 and the upper surface of the sample stage 2 is 75-85 mm.
进一步地，所述计算机7向P4系列LED阵列1发送触发信号，使P4系列LED阵列1交替产生两个半径为r、圆心间距为d、颜色相同的圆形照明光源，其中r、d为像素点个数。Further, the computer 7 sends a trigger signal to the P4 series LED array 1, so that the P4 series LED array 1 alternately generates two circular lighting sources with a radius of r, a distance between centers of circles of d, and the same color, where r and d are pixel count.
优选地，所述摄像机5的CCD镜头位于镜筒透镜4的后焦面上。Preferably, the CCD lens of the camera 5 is located on the rear focal plane of the barrel lens 4 .
进一步地，所述计算机7向摄像机5发送的触发信号与向P4系列LED阵列1发送的触发信号保持同步。Further, the trigger signal sent by the computer 7 to the camera 5 is synchronized with the trigger signal sent to the P4 series LED array 1 .
本发明基于权利要求1所述装置的偏振式动态实时体视显微成像方法，步骤如下：The present invention is based on the polarized dynamic real-time stereomicroscopic imaging method of the device described in claim 1, the steps are as follows:
步骤1，将待成像的样品置于样品载物台2，计算机7向P4系列LED阵列1发送触发信号，使P4系列LED阵列1交替产生两个圆形照明光；Step 1, place the sample to be imaged on the sample stage 2, and the computer 7 sends a trigger signal to the P4 series LED array 1, so that the P4 series LED array 1 alternately generates two circular illumination lights;
步骤2，步骤1产生的照明光透过样品载物台2被显微物镜3收集，显微物镜3将收集的照明光进行放大成像后入射至镜筒透镜4；Step 2, the illumination light generated in step 1 passes through the sample stage 2 and is collected by the microscope objective lens 3, and the microscope objective lens 3 magnifies the collected illumination light and then enters the tube lens 4;
步骤3，计算机7向驱动摄像机5发送触发信号以驱动摄像机5对穿过镜筒透镜4的样品图像进行采样，得到分别对应于两个圆形照明光的显微样品图像；Step 3, the computer 7 sends a trigger signal to the drive camera 5 to drive the camera 5 to sample the sample image passing through the barrel lens 4, and obtain microscopic sample images respectively corresponding to two circular illumination lights;
步骤4，摄像机5将步骤3拍摄到的两个显微样品图像输入偏振式3D显示器6分别作为左、右眼图像进行显示；In step 4, the camera 5 inputs the two microscopic sample images captured in step 3 into the polarized 3D display 6 as left and right eye images for display;
步骤5，观察者通过佩戴偏振式3D眼镜8观看偏振式3D显示器6即可实时观看到样品图像的立体显微结构。In step 5, the observer can view the stereoscopic microstructure of the sample image in real time by wearing the polarized 3D glasses 8 and looking at the polarized 3D display 6 .
进一步地，步骤1所述P4系列LED阵列1交替产生两个圆形照明光，该两个圆形照明光的半径为r、圆心间距为d、颜色相同，其中r、d为像素点的个数。Further, the P4 series LED array 1 described in step 1 alternately generates two circular lighting lights, the radius of the two circular lighting lights is r, the distance between the centers of the circles is d, and the color is the same, where r and d are the number of pixels number.
进一步地，步骤3所述计算机7向摄像机5发送的触发信号与步骤1中计算机7向P4系列LED阵列1发送的触发信号保持同步。Further, the trigger signal sent by the computer 7 to the camera 5 in step 3 is synchronized with the trigger signal sent by the computer 7 to the P4 series LED array 1 in step 1 .
实施例1Example 1
结合图1，本发明偏振式动态实时体视显微成像装置，包括P4系列LED阵列1、样品载物台2、显微物镜3、镜筒透镜4、摄像机5、偏振式3D显示器6、计算机7和偏振式3D眼镜8；其中P4系列LED阵列1、样品载物台2、显微物镜3、镜筒透镜4、摄像机5从下至上依次设置，且P4系列LED阵列1的中心位于显微物镜3、镜筒透镜4的中心轴线上；P4系列LED阵列1和摄像机5的控制端均与计算机7连接，摄像机5的输出端接入偏振式3D显示器6；1, the polarized dynamic real-time stereomicroscopic imaging device of the present invention includes a P4 series LED array 1, a sample stage 2, a microscopic objective lens 3, a barrel lens 4, a camera 5, a polarized 3D display 6, and a computer 7 and polarized 3D glasses 8; wherein the P4 series LED array 1, the sample stage 2, the microscope objective lens 3, the lens barrel lens 4, and the camera 5 are arranged in sequence from bottom to top, and the center of the P4 series LED array 1 is located in the microscope On the central axis of the objective lens 3 and the lens barrel lens 4; the control ends of the P4 series LED array 1 and the camera 5 are connected to the computer 7, and the output end of the camera 5 is connected to a polarized 3D display 6;
将待成像的样品置于样品载物台2，计算机7向P4系列LED阵列1发送触发信号，使P4系列LED阵列1交替产生两个圆形照明光，颜色任意，只要保证两个圆形照明光颜色相同即可，如图2所示，(a)为初始化时P4系列LED阵列产生的白色圆形照明光示意图示意图，(b)为某一时刻P4系列LED阵列产生的白色圆形照明光示意图，(c)为下一时刻P4系列LED阵列产生的白色圆形照明光示意图。照明光透过样品载物台2被显微物镜3收集，显微物镜3将收集的照明光进行放大成像后入射至镜筒透镜4，计算机7向驱动摄像机5发送触发信号以驱动摄像机5对穿过镜筒透镜4的样品图像进行采样，摄像机5将采集的样品图像输入偏振式3D显示器6进行显示，观察者通过佩戴偏振式3D眼镜8观看偏振式3D显示器6即可实时观看到样品图像的立体显微结构。Place the sample to be imaged on the sample stage 2, and the computer 7 sends a trigger signal to the P4 series LED array 1, so that the P4 series LED array 1 alternately generates two circular illumination lights with any color, as long as the two circular illumination lights are guaranteed The light color is the same, as shown in Figure 2, (a) is a schematic diagram of the white circular lighting light generated by the P4 series LED array during initialization, and (b) is the white circular lighting light generated by the P4 series LED array at a certain moment Schematic diagram, (c) is a schematic diagram of the white circular illumination light generated by the P4 series LED array at the next moment. The illumination light passes through the sample stage 2 and is collected by the microscope objective lens 3. The microscope objective lens 3 magnifies the collected illumination light and then enters the lens barrel lens 4. The computer 7 sends a trigger signal to the drive camera 5 to drive the camera 5 pair The sample image passed through the barrel lens 4 is sampled, and the camera 5 inputs the collected sample image to the polarized 3D display 6 for display, and the observer can watch the sample image in real time by wearing the polarized 3D glasses 8 and viewing the polarized 3D display 6 stereoscopic microstructure.
优选地，所述P4系列LED阵列1提供红色、绿色、蓝色、青色、粉色、黄色、白光七种颜色的照明光，本装置采用任意颜色照明光均可，只要保证两个圆形照明光源颜色相同即可；P4系列LED阵列1的单元板尺寸为128mm*128mm、像素个数为32*32、像素间距4mm，每个像素点均可单独点亮。Preferably, the P4 series LED array 1 provides seven colors of illumination light: red, green, blue, cyan, pink, yellow, and white. The device can use any color of illumination light, as long as two circular illumination sources are guaranteed The same color is enough; the unit board size of P4 series LED array 1 is 128mm*128mm, the number of pixels is 32*32, and the pixel pitch is 4mm. Each pixel can be lighted up independently.
优选地，所述P4系列LED阵列1距离样品载物台2上表面的距离为75～85mm。Preferably, the distance between the P4 series LED array 1 and the upper surface of the sample stage 2 is 75-85 mm.
进一步地，所述计算机7向P4系列LED阵列1发送触发信号，使P4系列LED阵列1交替产生两个半径为r、圆心间距为d、颜色相同的圆形照明光源，其中r、d为像素点个数。如图3所示，控制LED阵列1的两个白色圆形照明光源的半径r和圆心间距d即可实现对焦深和体视角的灵活可调。假设左边白色圆形照明光源的半径为r，圆心位于A点处；右边白色圆形照明光源的半径为r，圆心位于B点处，O点为AB的中点，位于显微物镜、透镜的中心轴线。OC即P4系列LED阵列1与样品载物台2的距离，用h表示，一般为80mm左右。通过控制两个圆心的水平间隔为d可实现对体视角θ的控制：θ＝arctand/2h；通过控制两个圆心的半径r可实现对于照明数值孔径角θNA的控制：θNA≈θ′＝arctan r/h，数值孔径角θNA反比于焦深。Further, the computer 7 sends a trigger signal to the P4 series LED array 1, so that the P4 series LED array 1 alternately generates two circular lighting sources with a radius of r, a distance between centers of circles of d, and the same color, where r and d are pixel count. As shown in FIG. 3 , by controlling the radius r and the distance d between the centers of the two white circular illumination sources of the LED array 1 , the flexible adjustment of the depth of focus and the volume viewing angle can be realized. Assume that the radius of the left white circular lighting source is r, and the center of the circle is at point A; the radius of the right white circular lighting source is r, and the center of the circle is at point B, and the point O is the midpoint of AB, which is located between the microscope objective lens and the lens. central axis. OC is the distance between the P4 series LED array 1 and the sample stage 2, represented by h, generally about 80mm. By controlling the horizontal interval between the two centers of circles as d, the control of the volume viewing angle θ can be realized: θ=arctand/2h; by controlling the radius r of the two centers of circles, the control of the illumination numerical aperture angle θNA can be realized: θNA ≈θ′ =arctan r/h, the numerical aperture angle θNA is inversely proportional to the depth of focus.
优选地，摄像机5的CCD镜头位于镜筒透镜4的后焦面上。Preferably, the CCD lens of the camera 5 is located on the rear focal plane of the barrel lens 4 .
进一步地，所述计算机7向摄像机5发送的触发信号与向P4系列LED阵列1发送的触发信号保持同步。Further, the trigger signal sent by the computer 7 to the camera 5 is synchronized with the trigger signal sent to the P4 series LED array 1 .
本发明偏振式动态实时体视显微成像方法，步骤如下：The polarized dynamic real-time stereomicroscopic imaging method of the present invention has the following steps:
步骤1，将待成像的样品置于样品载物台2，计算机7向P4系列LED阵列1发送触发信号，使P4系列LED阵列1交替产生两个圆形照明光；该两个圆形照明光的半径为r、圆心间距为d、颜色相同，其中r、d为像素点的个数。Step 1, the sample to be imaged is placed on the sample stage 2, and the computer 7 sends a trigger signal to the P4 series LED array 1, so that the P4 series LED array 1 alternately generates two circular illumination lights; the two circular illumination lights The radius is r, the distance between the centers is d, and the color is the same, where r and d are the number of pixels.
步骤2，步骤1产生的照明光透过样品载物台2被显微物镜3收集，显微物镜3将收集的照明光进行放大成像后入射至镜筒透镜4。Step 2. The illumination light generated in step 1 passes through the sample stage 2 and is collected by the microscope objective lens 3 .
步骤3，计算机7向驱动摄像机5发送触发信号以驱动摄像机5对穿过镜筒透镜4的样品图像进行采样，得到分别对应于两个圆形照明光的显微样品图像；所述计算机7向摄像机5发送的触发信号与步骤1中计算机7向P4系列LED阵列1发送的触发信号保持同步。Step 3, the computer 7 sends a trigger signal to the drive camera 5 to drive the camera 5 to sample the sample image passing through the barrel lens 4, and obtain microscopic sample images corresponding to two circular illumination lights respectively; The trigger signal sent by the camera 5 is synchronized with the trigger signal sent by the computer 7 to the P4 series LED array 1 in step 1.
步骤4，摄像机5将步骤3拍摄到的两个显微样品图像输入偏振式3D显示器6分别作为左、右眼图像进行显示；In step 4, the camera 5 inputs the two microscopic sample images captured in step 3 into the polarized 3D display 6 as left and right eye images for display;
步骤5，观察者通过佩戴偏振式3D眼镜8观看偏振式3D显示器6即可实时观看到样品图像的立体显微结构。图4是本实施例中摄像机所拍摄到的样品图。In step 5, the observer can view the stereoscopic microstructure of the sample image in real time by wearing the polarized 3D glasses 8 and looking at the polarized 3D display 6 . Fig. 4 is a sample diagram captured by the camera in this embodiment.
本发明中P4系列LED阵列的使用，取代了传统立体显微镜的双光路，简化了系统设计，不仅使成像系统简便而且有效降低成本；通过采用LED阵列作为照明光源，具有系统本身的光源，受外界干扰较小，观察条件的限制性降低，在暗背景下能够进行观看；通过控制P4系列LED阵列上的圆形照明光源的半径r和圆心距离d分别实现对焦深和体视角的灵活可调；将摄像机采集到的图像传到偏振式3D显示器上，从而可以实现对样品立体结构的动态实时清晰观看，克服红蓝立体成像的颜色效果差的缺点，提升观察者体验度。The use of the P4 series LED array in the present invention replaces the dual optical path of the traditional stereo microscope, simplifies the system design, not only makes the imaging system simple but also effectively reduces the cost; by using the LED array as the lighting source, it has the light source of the system itself, which is affected by the outside world. The interference is small, the restriction of observation conditions is reduced, and viewing can be performed in a dark background; by controlling the radius r and the center distance d of the circular lighting source on the P4 series LED array, the depth of focus and the stereoscopic viewing angle can be flexibly adjusted; The image collected by the camera is transmitted to the polarized 3D display, so that the dynamic real-time and clear viewing of the three-dimensional structure of the sample can be realized, the disadvantage of poor color effect of red and blue three-dimensional imaging can be overcome, and the experience of the observer can be improved.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510811945.6ACN106772981A (en) | 2015-11-20 | 2015-11-20 | The stereoscopic microscopic imaging device of polarization type dynamic realtime and method |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510811945.6ACN106772981A (en) | 2015-11-20 | 2015-11-20 | The stereoscopic microscopic imaging device of polarization type dynamic realtime and method |
| Publication Number | Publication Date |
|---|---|
| CN106772981Atrue CN106772981A (en) | 2017-05-31 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510811945.6APendingCN106772981A (en) | 2015-11-20 | 2015-11-20 | The stereoscopic microscopic imaging device of polarization type dynamic realtime and method |
| Country | Link |
|---|---|
| CN (1) | CN106772981A (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110955039A (en)* | 2019-11-15 | 2020-04-03 | 上海安翰医疗技术有限公司 | Phase contrast microscopic imaging system and imaging method thereof |
| CN115398312A (en)* | 2020-03-02 | 2022-11-25 | 卡尔蔡司医疗技术股份公司 | Head-mounted visualization unit and visualization system |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4207630A1 (en)* | 1992-03-11 | 1993-09-23 | Kappa Messtechnik Gmbh | Stereo microscope for light section microscopy - has light source and eyepiece in one path, and CCD camera in other |
| CN101900875A (en)* | 2010-06-04 | 2010-12-01 | 南开大学 | High-magnification three-dimensional imaging microscope and imaging method based on off-axis illumination of dual light sources |
| CN103376540A (en)* | 2012-04-29 | 2013-10-30 | 麦克奥迪实业集团有限公司 | Stereoscopic microscope LED coaxial lighting device |
| CN103792654A (en)* | 2014-01-26 | 2014-05-14 | 中国科学院苏州生物医学工程技术研究所 | Structured light lighting optical system |
| CN104765138A (en)* | 2015-04-17 | 2015-07-08 | 南京理工大学 | Multi-mode micro-imaging system and method based on LED array |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4207630A1 (en)* | 1992-03-11 | 1993-09-23 | Kappa Messtechnik Gmbh | Stereo microscope for light section microscopy - has light source and eyepiece in one path, and CCD camera in other |
| CN101900875A (en)* | 2010-06-04 | 2010-12-01 | 南开大学 | High-magnification three-dimensional imaging microscope and imaging method based on off-axis illumination of dual light sources |
| CN103376540A (en)* | 2012-04-29 | 2013-10-30 | 麦克奥迪实业集团有限公司 | Stereoscopic microscope LED coaxial lighting device |
| CN103792654A (en)* | 2014-01-26 | 2014-05-14 | 中国科学院苏州生物医学工程技术研究所 | Structured light lighting optical system |
| CN104765138A (en)* | 2015-04-17 | 2015-07-08 | 南京理工大学 | Multi-mode micro-imaging system and method based on LED array |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110955039A (en)* | 2019-11-15 | 2020-04-03 | 上海安翰医疗技术有限公司 | Phase contrast microscopic imaging system and imaging method thereof |
| CN110955039B (en)* | 2019-11-15 | 2022-10-14 | 上海安翰医疗技术有限公司 | Phase contrast microscopic imaging system and imaging method thereof |
| CN115398312A (en)* | 2020-03-02 | 2022-11-25 | 卡尔蔡司医疗技术股份公司 | Head-mounted visualization unit and visualization system |
| US12153220B2 (en) | 2020-03-02 | 2024-11-26 | Carl Zeiss Meditec Ag | Head-mounted visualization system |
| CN115398312B (en)* | 2020-03-02 | 2025-05-06 | 卡尔蔡司医疗技术股份公司 | Head mounted visualization unit and visualization system |
| Publication | Publication Date | Title |
|---|---|---|
| JP4991211B2 (en) | Stereo microscope | |
| CN203522943U (en) | Image acquisition device and 3D display system | |
| KR20140036125A (en) | Stereoscopic optics | |
| US20180344154A1 (en) | Wide angle stereoscopic funduscopy | |
| CN106406016A (en) | Three-dimensional imaging device, three-dimensional imaging system and three-dimensional imaging method | |
| JP2017079904A (en) | Surgical microscope, image processing device, and image processing method | |
| JPWO2012105157A1 (en) | Stereoscopic image capturing apparatus and endoscope | |
| JP2013106189A (en) | Imaging apparatus | |
| CN106772982A (en) | A kind of stereoscopic microscopic imaging device and method | |
| CN105158889B (en) | Stereoscopic microscopic imaging device and its method based on LED array illumination | |
| WO2019223090A1 (en) | Intelligent mixed reality telescope | |
| RU2340116C1 (en) | Method of playing back stereoscopic images from camcorder with converging axes | |
| CN106772981A (en) | The stereoscopic microscopic imaging device of polarization type dynamic realtime and method | |
| JP2018152748A (en) | Imaging/display device of stereoscopic image and head mount device | |
| CN106610522A (en) | Three-dimensional microscopic imaging device and method | |
| JP2011191517A (en) | Stereomicroscope and control device for microscope | |
| KR101859197B1 (en) | Real-time stereoscopic microscope | |
| CN106772980A (en) | The stereoscopic microscopic imaging device of shutter dynamic realtime and method | |
| US20160116726A1 (en) | Three-dimensional stereoscopic microscope | |
| CN105049833B (en) | A kind of stereopsis inspection method and device using symmetrical adjustable backlight structure | |
| CN106680271A (en) | Spatial light modulator-based naked eye stereoscopic microimaging apparatus and method | |
| JP5857782B2 (en) | Imaging device | |
| CN107132649A (en) | Surgical operation microscope 3D imaging devices | |
| JP3816589B2 (en) | Stereoscopic endoscope | |
| US20160363852A1 (en) | Single axis stereoscopic imaging apparatus with dual sampling lenses |
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication | Application publication date:20170531 |