技术领域technical field
本发明涉及医疗器械及肿瘤学领域。涉及一种新型的医用穿刺活检针，具体涉及一种带有光纤探针的穿刺活检针。本发明在原有的穿刺活检针的基础上，加入了拉曼光纤探针的设计，可以通过实时检测拉曼光谱的差异智能识别肿瘤组织与正常组织；进一步可用于快速诊断良恶性肿瘤。The invention relates to the fields of medical equipment and oncology. The invention relates to a novel medical puncture biopsy needle, in particular to a puncture biopsy needle with an optical fiber probe. On the basis of the original puncture biopsy needle, the present invention adds the design of Raman optical fiber probe, which can intelligently identify tumor tissue and normal tissue by detecting the difference of Raman spectrum in real time; further, it can be used for rapid diagnosis of benign and malignant tumors.
背景技术Background technique
现有技术公开了肿瘤病理学诊断是肿瘤治疗的前提。穿刺活检是获得组织病理标本的主要途径。通常尽管穿刺活检具有快速、易行、准确、安全、经济的特点，但由于病变所处位置及其结构的异质性，使得临床上穿刺活检取材的准确性及穿刺的安全性受到影响，给临床治疗带来很大难题。临床实践显示，目前的各种穿刺活检针尚不能实时识别肿瘤组织和正常组织，仅凭借医生的经验，较难精准分清肿瘤组织与正常组织的界限，因此制约着活检准确性，往往需要多次活检才能获得确诊。The prior art discloses that tumor pathological diagnosis is a prerequisite for tumor treatment. Needle biopsy is the main way to obtain histopathological specimens. Generally, although needle biopsy is fast, easy, accurate, safe, and economical, due to the heterogeneity of the location and structure of the lesion, the accuracy of needle biopsy and the safety of needle biopsy are affected clinically. Clinical treatment poses a big problem. Clinical practice shows that the current various biopsy needles cannot identify tumor tissue and normal tissue in real time. Only relying on the experience of doctors, it is difficult to accurately distinguish the boundary between tumor tissue and normal tissue. This restricts the accuracy of biopsy and often requires multiple A biopsy is required to confirm the diagnosis.
自90年代以来，随着激光技术的发展及新型探测器技术的应用逐渐成熟，拉曼光谱技术的应用越来越广泛；由于人体器官组织包括肿瘤组织就其基本化学组成均是由蛋白质，核酸，脂类和糖类等生物分子组成，不同结构的分子具有不同的拉曼光谱特征，因而可从物质的拉曼特征光谱来识别和区分不同的物质。根据肿瘤组织与正常组织中各成分的构成比例和分子构象上的差异,使得通过临床光纤拉曼光谱技术捕获拉曼散射光谱的差异，直接用在体内实时识别恶性肿瘤与正常组织成为可能。近年来，有研究发现将遗传算法应用于拉曼光谱分析中，可以对早期癌性组织做活体快速识别；研究者分析识别宫颈癌组织得到的拉曼光谱带对癌早期诊断准确度82.9%（灵敏度72.5% (29/40)，特异性89.2% (58/65)）”。光纤拉曼光谱作为一种光学技术可提供组织样本的分子结构信息；P. Crow等分析了29例冰冻膀胱癌样本的220张拉曼光谱，结果表明光纤近红外拉曼光谱可用于膀胱移行细胞癌的体外诊断。Since the 1990s, with the development of laser technology and the application of new detector technology, the application of Raman spectroscopy has become more and more extensive; since the basic chemical composition of human organs and tissues, including tumor tissues, are composed of proteins, nucleic acids , lipids and sugars and other biomolecules, molecules with different structures have different Raman spectral characteristics, so different substances can be identified and distinguished from the Raman characteristic spectra of substances. According to the composition ratio and molecular conformation differences between tumor tissue and normal tissue, it is possible to capture the difference of Raman scattering spectrum through clinical optical fiber Raman spectroscopy technology, and directly use it in vivo to identify malignant tumor and normal tissue in real time. In recent years, studies have found that the application of genetic algorithms to Raman spectrum analysis can quickly identify early cancerous tissues in vivo; researchers analyzed and identified Raman spectrum bands obtained from cervical cancer tissues, and the accuracy of early cancer diagnosis was 82.9% ( Sensitivity 72.5% (29/40), specificity 89.2% (58/65))". Optical fiber Raman spectroscopy as an optical technique can provide molecular structure information of tissue samples; P. Crow et al analyzed 29 cases of frozen bladder cancer 220 Raman spectra of samples, the results show that fiber optic near-infrared Raman spectroscopy can be used for in vitro diagnosis of bladder transitional cell carcinoma.
基于现有技术的现状，本申请的发明人拟提供一种能实时识别癌变组织与正常组织的智能穿刺针，以达到尽可能的提高穿刺活检的精准度，进一步有助于快速诊断良恶性肿瘤。Based on the current state of the art, the inventor of this application intends to provide an intelligent puncture needle that can identify cancerous tissue and normal tissue in real time, so as to improve the accuracy of biopsy as much as possible, and further contribute to the rapid diagnosis of benign and malignant tumors .
发明内容Contents of the invention
本发明的目的是提供一种新型能识别肿瘤组织和细胞的智能穿刺活检针；具体涉及一种带有光纤探针的穿刺活检针。The purpose of the present invention is to provide a novel intelligent puncture biopsy needle capable of identifying tumor tissues and cells; in particular, it relates to a puncture biopsy needle with an optical fiber probe.
本发明的另一目的是应用该智能穿刺活检针用于术中实时识别癌变组织和正常组织，提高穿刺活检准确率，进一步有助于快速诊断良恶性肿瘤。Another object of the present invention is to use the intelligent puncture biopsy needle to identify cancerous tissue and normal tissue in real time during surgery, improve the accuracy of puncture biopsy, and further help to quickly diagnose benign and malignant tumors.
为达到上述目的，本发明采用下述技术方案：To achieve the above object, the present invention adopts the following technical solutions:
一种带光纤探针的穿刺活检针，主要包括控制释放激光和捕获、分析拉曼光谱的主机、套针内侧分布光纤探针的穿刺针及带有不同颜色信号灯和音频提示功能的声控触屏显示装置；在所述光纤探针后端连接激光光源和光谱分析仪，可以获得穿刺针内侧光纤探针尖端位置下组织的拉曼光谱信号，用以识别穿刺针尖所及组织是否癌变，并具有以多种形式的提醒功能。A puncture biopsy needle with an optical fiber probe, which mainly includes a host for controlling the release of laser light and capturing and analyzing Raman spectra, a puncture needle with optical fiber probes distributed inside the trocar, and a sound-activated touch screen with signal lights of different colors and audio prompts A display device; a laser light source and a spectrum analyzer are connected at the rear end of the optical fiber probe to obtain the Raman spectrum signal of the tissue at the tip of the optical fiber probe inside the puncture needle, so as to identify whether the tissue touched by the puncture needle tip is cancerous, and has Reminders in many forms.
更具体的，本发明的一种带有光纤探针的穿刺针，其特征在于，其主要由主机（1）、穿刺针（2）、光纤探针（3）显示器（4）组成，所述光纤探针（3）排列在穿刺针（2）针芯的外侧缘。More specifically, a puncture needle with an optical fiber probe according to the present invention is characterized in that it is mainly composed of a host (1), a puncture needle (2), an optical fiber probe (3) and a display (4). The fiber optic probe (3) is arranged on the outer edge of the needle core of the puncture needle (2).
本发明中，所述穿刺针包括：穿刺活检针、介入穿刺针、腰椎穿刺针等通过穿刺获得诊断或治疗的装置，本发明的一个实施例中，优选穿刺针为穿刺活检针。In the present invention, the puncture needles include: puncture biopsy needles, interventional puncture needles, lumbar puncture needles and other devices for obtaining diagnosis or treatment through puncture. In one embodiment of the present invention, the puncture needles are preferably puncture biopsy needles.
本发明中，所述主机（1）是控制激光释放及光谱捕获和分析功能的新型智能装置。In the present invention, the host (1) is a new type of intelligent device that controls the functions of laser release, spectrum capture and analysis.
本发明中，所述光纤探针（3）由锥形纳米光纤探针组成，其末端可以连接到可释放激光和捕获和分析拉曼光谱的主机。In the present invention, the optical fiber probe (3) is composed of a tapered nano-fiber optical probe, the end of which can be connected to a host that can release laser light and capture and analyze Raman spectra.
本发明中，所述光纤探针（3）还可由断面纳米光纤探针组成，其末端可以连接到可释放激光和捕获和分析拉曼光谱的主机。In the present invention, the optical fiber probe (3) can also be composed of cross-sectional nano-fiber optical probe, the end of which can be connected to a host that can release laser light and capture and analyze Raman spectra.
本发明中，所述显示器（4）是一种触屏式具有可同时辅以红黄绿颜色信号灯和音频提示功能。In the present invention, the display (4) is a touch-screen type, which can be supplemented with red, yellow and green color signal lights and audio prompt functions at the same time.
本发明中，所述显示器（4）是一种触屏式显示器，本发明的一个实施例中，优选触屏式显示器附加红、黄和绿色信号指示灯，其分别表示肿瘤组织、癌前病变和正常组织；本发明的一个实施例中，还优选触屏式显示器附加音频提醒功能。In the present invention, the display (4) is a touch-screen display. In one embodiment of the present invention, it is preferred that the touch-screen display has additional red, yellow and green signal indicator lights, which respectively represent tumor tissue and precancerous lesions. and normal tissue; in one embodiment of the present invention, it is also preferred that the touch-screen display has an additional audio reminder function.
本发明针对现有技术常用穿刺活检针无法自行识别肿瘤组织和正常组织而导致的一次穿刺活检成功率偏低的缺陷，提供了一种带有光纤探针的新型穿刺活检针，其套针内侧排布有光纤探针，可以通过光纤将激光信号传导至穿刺针尖的组织，对当前位置的组织进行拉曼光谱的实时监测和捕获，通过癌变组织和正常组织的拉曼光谱差异，实时识别癌变组织和正常组织，便于在术中保证准确取到肿瘤组织；该穿刺针还具有简单、易操作、使用方便和安全可靠等特点。The present invention aims at the defect that the conventional puncture biopsy needle cannot identify tumor tissue and normal tissue by itself, which results in a low success rate of puncture biopsy, and provides a novel puncture biopsy needle with an optical fiber probe. Arranged with fiber optic probes, the laser signal can be transmitted to the tissue at the puncture needle tip through the optical fiber, and the Raman spectrum of the tissue at the current position can be monitored and captured in real time, and the cancer can be identified in real time through the Raman spectrum difference between cancerous tissue and normal tissue Tissue and normal tissue, so as to ensure accurate access to tumor tissue during operation; the puncture needle also has the characteristics of simplicity, easy operation, convenient use, safety and reliability.
本发明与传统的穿刺针相比较，具有如下显而易见的突出实质性特点和显著优点：Compared with traditional puncture needles, the present invention has the following obvious outstanding substantive features and significant advantages:
1. 本发明的智能穿刺针应用光纤将激光传输至穿刺针尖端，从而对穿刺针尖处的组织拉曼光谱信号的收集，通过计算机计算并和不同组织拉曼光谱数据库比对分析在3秒钟内判断当前组织是否癌变，从而有助于外科医生实时判断穿刺过程中癌变组织的位置；通过分析对比拉曼光谱信号的来判断当前组织的癌变状态并显示于触摸显示屏，可分别以红色，黄色和绿色指示灯提示恶性组织，癌前病变和正常组织，同时辅以语音提示功能，提醒医生准确活检癌变组织。1. The intelligent puncture needle of the present invention uses optical fiber to transmit the laser light to the tip of the puncture needle, so that the collection of tissue Raman spectrum signals at the puncture needle tip can be judged within 3 seconds by computer calculation and comparison analysis with different tissue Raman spectrum databases Whether the current tissue is cancerous or not will help the surgeon to judge the position of the cancerous tissue in real time during the puncture process; the cancerous state of the current tissue can be judged by analyzing and comparing Raman spectrum signals and displayed on the touch screen, which can be displayed in red, yellow and The green indicator light prompts malignant tissue, precancerous lesions and normal tissue, and at the same time, it is supplemented by a voice prompt function to remind doctors to accurately biopsy cancerous tissue.
2. 本发明的新型智能穿刺针克服了当前常用穿刺针无法识别肿瘤组织和正常组织的不足；能彻底改变医生凭借肿瘤患者术前的影像学检查及个人的经验作出判断，不仅可以帮助医师精准获取癌变组织，还可以降低穿刺并发症。2. The new smart puncture needle of the present invention overcomes the deficiency that the current commonly used puncture needles cannot identify tumor tissue and normal tissue; Obtaining cancerous tissue can also reduce puncture complications.
3. 本发明的新型智能穿刺针通过实时拉曼光谱分析，具有替代细胞学涂片的诊断功能。3. The new intelligent puncture needle of the present invention has the diagnostic function of replacing cytological smears through real-time Raman spectrum analysis.
附图说明Description of drawings
图1是本发明的实施例中结合锥形纳米光纤探针的智能穿刺针的装置示意图。Fig. 1 is a schematic diagram of an intelligent puncture needle combined with a tapered nano-fiber probe in an embodiment of the present invention.
具体实施方式detailed description
本发明的优选实施例结合附图详述如下：Preferred embodiments of the present invention are described in detail as follows in conjunction with accompanying drawings:
实施例Example11
如图1所示，本发明的能识别肿瘤组织和细胞的智能穿刺针包括控制分析拉曼光谱的主机1和穿刺针2，在此基础上加设锥形纳米光纤探针3及显示器4，所述光纤探针（3）排列在穿刺针（2）针芯的外侧缘。As shown in Figure 1, the intelligent puncture needle capable of identifying tumor tissues and cells of the present invention includes a host computer 1 and a puncture needle 2 for controlling and analyzing Raman spectra, on which a tapered nano-fiber probe 3 and a display 4 are added, The fiber optic probe (3) is arranged on the outer edge of the needle core of the puncture needle (2).
本实施例中，穿刺针为穿刺活检针，主机（1）是控制激光释放及光谱捕获和分析功能的新型智能装置；光纤探针（3）由锥形纳米光纤探针组成，其末端可以连接到可释放激光和捕获和分析拉曼光谱的主机；所述显示器（4）为一种触屏式显示器，可附加红、黄和绿色信号指示灯，其分别表示肿瘤组织、癌前病变和正常组织；还可附加音频提醒功能；In this embodiment, the puncture needle is a puncture biopsy needle, and the host (1) is a new type of intelligent device that controls laser release and spectrum capture and analysis functions; the fiber optic probe (3) is composed of a tapered nanofiber fiber probe, and its end can be connected to the host computer that can release laser light and capture and analyze Raman spectra; the display (4) is a touch-screen display that can be attached with red, yellow and green signal indicator lights, which respectively represent tumor tissue, precancerous lesions and normal organization; audio reminders can also be added;
本实施例中所述锥形光纤探针3尖端尺寸能达到100纳米级，其分布在穿刺针针芯尖端外缘，其中光纤3终端可以连接具有激光光谱分析仪功能的主机1及显示器4；临床实施穿刺时，使用锥形光纤3连接到633nm等的激光光源，连接光源后，穿刺针接触到病理组织区域时，先通过光纤将激光打到穿刺针尖端的组织获取当前位置的拉曼光谱信号，通过分析对比拉曼光谱信号的判断当前组织的癌变状态并显示于触摸显示屏，可分别通过红色，黄色和绿色指示灯提示恶性组织，癌前病变和正常组织，同时辅以语音提示功能，提醒医生进行精准活检癌变组织，从而提高癌变组织的诊断正确率。The size of the tip of the tapered optical fiber probe 3 in this embodiment can reach 100 nanometers, and it is distributed on the outer edge of the needle core tip of the puncture needle, wherein the terminal of the optical fiber 3 can be connected to the host 1 and the display 4 with the function of a laser spectrum analyzer; When performing clinical puncture, use the tapered optical fiber 3 to connect to a laser light source such as 633nm. After connecting the light source, when the puncture needle touches the pathological tissue area, firstly use the optical fiber to hit the laser on the tissue at the tip of the puncture needle to obtain the Raman spectrum at the current position Signal, judge the cancerous state of the current tissue by analyzing and comparing the Raman spectrum signal and display it on the touch screen, which can prompt malignant tissue, precancerous lesions and normal tissue through red, yellow and green indicator lights respectively, supplemented by voice prompts , to remind doctors to perform accurate biopsy of cancerous tissue, so as to improve the accuracy of diagnosis of cancerous tissue.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510495347.2ACN106691549A (en) | 2015-08-13 | 2015-08-13 | Puncture biopsy needle with optical fiber probes |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510495347.2ACN106691549A (en) | 2015-08-13 | 2015-08-13 | Puncture biopsy needle with optical fiber probes |
| Publication Number | Publication Date |
|---|---|
| CN106691549Atrue CN106691549A (en) | 2017-05-24 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510495347.2APendingCN106691549A (en) | 2015-08-13 | 2015-08-13 | Puncture biopsy needle with optical fiber probes |
| Country | Link |
|---|---|
| CN (1) | CN106691549A (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107684441A (en)* | 2017-09-29 | 2018-02-13 | 黄志伟 | A kind of Raman microprobe device with fine needle aspiration biopsy's function |
| CN109394255A (en)* | 2018-12-14 | 2019-03-01 | 邱军 | A kind of intelligence hepatopathy check device |
| CN110916728A (en)* | 2019-12-10 | 2020-03-27 | 武汉大学中南医院 | Puncture biopsy method and device based on optical fiber transmission type fluorescence life guidance |
| CN111728674A (en)* | 2020-06-11 | 2020-10-02 | 武汉理工大学 | A Raman Scattering-Based Optical Fiber Probe Human Tissue Information Online Monitoring System |
| CN114601506A (en)* | 2022-03-30 | 2022-06-10 | 北京中研智创新技术研究院有限公司 | Brain tissue detection system based on artificial intelligence recognition and Raman spectrum |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104107069A (en)* | 2013-04-16 | 2014-10-22 | 南方医科大学南方医院 | Ultrasonic microscopic examination system for mediastinal lesions |
| CN104321018A (en)* | 2012-05-22 | 2015-01-28 | 皇家飞利浦有限公司 | Ultrasound image display set-up for remote display terminal |
| CN104541153A (en)* | 2012-07-02 | 2015-04-22 | 新加坡国立大学 | Methods related to real-time cancer diagnostics at endoscopy utilizing fiber-optic raman spectroscopy |
| CN204909588U (en)* | 2015-08-13 | 2015-12-30 | 复旦大学附属肿瘤医院 | Aspiration biopsy needle with optical fiber probe |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104321018A (en)* | 2012-05-22 | 2015-01-28 | 皇家飞利浦有限公司 | Ultrasound image display set-up for remote display terminal |
| CN104541153A (en)* | 2012-07-02 | 2015-04-22 | 新加坡国立大学 | Methods related to real-time cancer diagnostics at endoscopy utilizing fiber-optic raman spectroscopy |
| CN104107069A (en)* | 2013-04-16 | 2014-10-22 | 南方医科大学南方医院 | Ultrasonic microscopic examination system for mediastinal lesions |
| CN204909588U (en)* | 2015-08-13 | 2015-12-30 | 复旦大学附属肿瘤医院 | Aspiration biopsy needle with optical fiber probe |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107684441A (en)* | 2017-09-29 | 2018-02-13 | 黄志伟 | A kind of Raman microprobe device with fine needle aspiration biopsy's function |
| CN109394255A (en)* | 2018-12-14 | 2019-03-01 | 邱军 | A kind of intelligence hepatopathy check device |
| CN109394255B (en)* | 2018-12-14 | 2019-09-03 | 邱军 | A kind of intelligence hepatopathy check device |
| CN110916728A (en)* | 2019-12-10 | 2020-03-27 | 武汉大学中南医院 | Puncture biopsy method and device based on optical fiber transmission type fluorescence life guidance |
| CN111728674A (en)* | 2020-06-11 | 2020-10-02 | 武汉理工大学 | A Raman Scattering-Based Optical Fiber Probe Human Tissue Information Online Monitoring System |
| CN114601506A (en)* | 2022-03-30 | 2022-06-10 | 北京中研智创新技术研究院有限公司 | Brain tissue detection system based on artificial intelligence recognition and Raman spectrum |
| Publication | Publication Date | Title |
|---|---|---|
| CN106420041A (en) | High frequency electric operating knife with fiber probe | |
| US9226731B2 (en) | Optically guided needle biopsy system using multi-modal spectroscopy in combination with a transrectal ultrasound probe | |
| Evers et al. | Diffuse reflectance spectroscopy: a new guidance tool for improvement of biopsy procedures in lung malignancies | |
| CN106691549A (en) | Puncture biopsy needle with optical fiber probes | |
| WO2017206201A1 (en) | Fiberscope-based raman spectrum inspection device and method for implementing same | |
| CN104114075B (en) | There is the photon probe unit of integrated organization marking arrangement | |
| JP6398093B2 (en) | Detection of atherosclerotic plaque | |
| CN204909588U (en) | Aspiration biopsy needle with optical fiber probe | |
| JP2013514520A (en) | Apparatus and method for in vivo tissue characterization by Raman spectroscopy | |
| EP2525704A1 (en) | Apparatus and methods for characterization of lung tissue by raman spectroscopy | |
| CN204909631U (en) | High frequency operation electrotome with optical fiber probe | |
| Villard et al. | Confocal laser endomicroscopy and confocal microscopy for head and neck cancer imaging: Recent updates and future perspectives | |
| US20160030022A1 (en) | Optical Biopsy Needle and Endoscope System | |
| Spliethoff et al. | In vivo characterization of colorectal metastases in human liver using diffuse reflectance spectroscopy: toward guidance in oncological procedures | |
| CN110840397A (en) | Endoscopic Raman spectrum detection device for intracavity tissue | |
| Neto et al. | Micro-Raman spectroscopic study of thyroid tissues | |
| CN211749542U (en) | Endoscopic Raman spectrum detection device for intracavity tissue | |
| Salomon et al. | The feasibility of prostate cancer detection by triple spectroscopy | |
| Farah et al. | Advances in early detection and diagnostic adjuncts in oral cavity cancer | |
| Karthika et al. | Label‐free assessment of the transformation zone using multispectral diffuse optical imaging toward early detection of cervical cancer | |
| US20240122494A1 (en) | Techniques for assessing cancerous tissue during surgical biopsies | |
| JP6712550B2 (en) | System and method for detecting underlying tissue anomalies | |
| US20150182204A1 (en) | System and method for attaching a biopsy collecting device to a spectroscopy system | |
| CN114366037B (en) | Spectral diagnosis system for guiding in brain cancer operation and working method thereof | |
| Gaikwad et al. | Advancement in Diagnostic aids for oral premalignant lesions: A review |
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | Application publication date:20170524 | |
| RJ01 | Rejection of invention patent application after publication |