Automatic clinical chemistry analyzer and biochemical detection systemTechnical field
The invention belongs to medical test detection technique field, particularly automatic clinical chemistry analyzer and biochemical detection system.
Background technology
Biochemistry analyzer is the detection equipment that medical institutions are conventional.The reagent distribution of current automatic clinical chemistry analyzer is allThe system of the diluter composition using the reagent pin shared and share performs reagent distribution, i.e. by reagent pin in the driving of diluterA certain amount of reagent first drawn by lower reagent pin in reagent position, and then reagent pin moves to detect cup, and being driven by diluter will examinationReagent in agent pin joins in detection cup, additionally uses shared mixing needle to be stirred the liquid in detection cup.
Shared reagent pin, mixing needle is used to contact different reagent respectively during the detection of existing automatic clinical chemistry analyzer, althoughBefore exchanging other reagent for, instrument needs to be carried out inside and outside reagent pin and outside mixing needle, but is difficult to thoroughly stopThe residual of the different reagent of reagent pin, mixing needle contact, is therefore easily caused the cross-contamination between reagent.For eliminating instrument reagentThe reagent residual contamination of pin and mixing needle, especially reagent pin interior reagent residual, generally requires the substantial amounts of water of use and cleaningAgent is carried out, and otherwise, the reagent sucking the next one can be produced severe contamination by remaining reagent.In addition biochemistry analyzer is in inspectionAlso needing to during survey mix the reactant in detection cup, could obtain good testing result, conventional agitating device is alsoIt is shared contact agitator, and owing to contact stirring necessarily causes existing the risk of cross-contamination, existing full-automatic lifeFractional analysis instrument is to reduce this kind of cross-contaminations formed between reagent, sample due to difference, frequently with consuming a large amount of water to reagentPin, mixing needle are carried out, but often clean not thoroughly owing to instrument speed limits, and also produce substantial amounts of waste water.Due to respectivelyDetection cup random detection disparity items, therefore contacts different reagent and there is also the wind of reagent residual in various degree in detection cupDanger.
Summary of the invention
It is an object of the invention to provide a kind of automatic clinical chemistry analyzer and biochemical detection system.
The technical scheme realizing the object of the invention is: a kind of automatic clinical chemistry analyzer, and this analyser includes detecting cup, anti-Should coil, detect cup cleaning device, more than one reagent set, mixing needle, sample pin and specimen disc；
Described reaction tray is used for placing detection cup；Described detection cup cleans device and is used for cleaning detection cup；Described specimen discFor placing testing sample pipe；Described sample pin sample on pipette samples dish is to detecting in cup；
Each reagent set is made up of multiple reagent unit, and reagent unit is for adding fixating reagent in correspondence detection cup；Each reagent unit includes a reagent bottle, level sensor, reagent transfer device, first reagent pin and a connecting line,Reagent is to detecting cup for driving in the first reagent pin extraction reagent bottle for described reagent transfer device, and level sensor is used for detectingWith or without reagent in connecting line；
Described mixing needle is liquid in stirring and evenly mixing detection cup.
A kind of full-automatic biochemical detecting system, this system includes multiple biochemistry analyzer, main control computer, sample treatment listUnit, sample transfer unit and sample collection unit；Described each biochemistry analyzer has been used for disparity items detection；Described master control electricityBrain is used for controlling biochemistry analyzer, sample treatment unit, sample transfer unit and sample collection unit work；Described sample treatmentUnit is used for centrifugal treating testing sample, automatically opens up sample lid and put the most successively to sample transfer unit；Described sampleProduct transfer unit is for transmitting sample between biochemistry analyzer；Described sample collection unit is used for collecting sample after detection.
Compared with prior art, the remarkable advantage of the present invention is:
(1) one pipeline of each reagent bottle separate connection in the reagent set of the present invention, and each reagent cartridge only shifts examination of the same raceAgent, there is not the cross-contamination of different reagent, need not the most at work reagent pin and pipeline in each reagent cartridge, reagent pinClean；(2) present invention is by reactant in contactless air pressure mixing needle stirring detection cup, decreases the intersection that mixing needle causesPollute；(3) present invention takes each detection cup only cycle detection identical items, and not other project of cross detection, reduce furtherIt is to remain the cross-contamination caused that the different reagent place of instrument detection cup contact is formed；(4) during the present invention greatly reduces instrument operationRequired abluent and the consumption of water, decrease the generation of waste liquid the most accordingly.
Accompanying drawing explanation
Fig. 1 is the biochemical instruments structural representation using circular sample dish.
Fig. 2 is the biochemical instruments structural representation using rail mounted specimen holder.
The full automatic biochemical apparatus that Fig. 3 designs for the present invention use an air pump realize negative pressure waste discharge liquid and malleation simultaneouslyThe apparatus structure schematic diagram of air pressure supply blender gas pressing source of the gas.
Fig. 4 (a) be contactless air pressure mixing needle when producing impact air pressure on the left of liquid level in detection cup liquid flowing showBeing intended to, Fig. 4 (b) is that contactless air pressure mixing needle stops liquid backflow schematic diagram after pressure, and Fig. 4 (c) is for detecting cup after stirringMiddle liquid level schematic diagram.
Fig. 5 is that reagent pin of the present invention exports proper alignment structural representation.
Fig. 6 is shared reagent pin outlet mobile device and the reagent pin outlet arrangements schematic diagram of the present invention.
Fig. 7 is a reagent unit structural representation of the present invention.
Fig. 8 is the schematic diagram of the streamline biochemical detection system of the multiple stage biochemistry analyzer composition of the present invention.
Fig. 9 is the biochemistry analyzer schematic diagram in one embodiment of the invention.
Detailed description of the invention
In conjunction with Fig. 1 and Fig. 6, a kind of automatic clinical chemistry analyzer of the present invention, this analyser includes detecting cup 14, reaction tray1, detection cup cleans device 3, more than one reagent set, mixing needle, sample pin 6 and specimen disc 8；
Described reaction tray 1 is used for placing detection cup 14；Described detection cup cleans device 3 and is used for cleaning detection cup；Described sampleProduct dish 8 is used for placing testing sample pipe；The described sample pin 6 sample on pipette samples dish is to detecting in cup；
Each reagent set is made up of multiple reagent unit, and reagent unit is for adding fixating reagent in correspondence detection cup；Each reagent unit includes a reagent bottle 16,17, reagent transfer device the 18, first reagent pin N of level sensor and companyAdapter road, described reagent transfer device 18 is for driving reagent extremely detection cup 14, liquid level in the first reagent pin N extraction reagent bottle 16Detector 17 is used for detecting in connecting line with or without reagent；
Described mixing needle is liquid in stirring and evenly mixing detection cup.
Further, described each reagent set also includes a shared reagent pin mobile device, for by each for this group reagent pinExport and need to move above the detection cup specified according to detection.
Further, the reagent pin reagent exit of each reagent unit is neatly arranged on same straight line or camber line.
Further, described mixing needle is contactless air pressure mixing needle, the gas outlet of contactless air pressure mixing needleLiquid level in direct detection cup；Described contactless air pressure mixing needle connects free air pump and pressure-regulating device, described air pumpFor blasting air and being sprayed by contactless air pressure mixing needle, described pressure-regulating device is used for regulating contactless air pressureAir-flow size, speed and the time of mixing needle ejection.
Further, contactless air pressure mixing needle periphery is provided with heating and temperature regulating device, for contactless gasIn pressure mixing needle, gas carries out heating and thermostatic control, is controlled in the range of 20-45 DEG C by air pressure mixing needle effluent air temp.
Further, the reagent pipetting volume hole site in described reaction tray is provided with the lid of belt switch, when not reagent addingCover closing, when needs reagent adding, lid is opened.
Further, this analyser is additionally provided with reagent catcher, for collecting a small amount of reagent that reagent pin mouth is discharged.CauseIt is that Confirmation reagent has been delivered to, at reagent pin mouth, to discharge a small amount of reagent for reagent pin when just accessing reagent.
Further, described specimen disc is circular sample dish 8 or rail mounted specimen holder 7.
Further, this analyser also includes a reagent disc 22 and a second reagent pin 21, and described reagent disc 22 is usedIn placing multiple reagent bottle, described second reagent pin 21 is for sharing reagent pin, for by reagent in reagent bottle each on reagent disc 22It is transferred to detect in cup.
Further, it is provided with baffle plate below described reagent pin and controls the motor of flapper.The gear when adding reagentPlate is removed, and when not reagent adding, baffle plate crosspiece is under reagent pin, it is to avoid reagent instills in detection cup by mistake.
Further, described reagent transfer device 18 is syringe, quantitative peristaltic pump or quantitative shifting pump.
Further, described reagent position also sets up cold storage plant and temperature regulating device, controls temperature of reagent and is maintained at 2-15 DEG CScope.
Further, described reagent pin periphery is provided with device of rising again, it is ensured that cold preservation reagent temperature when adding detection cup reachesTo more than 20 DEG C.
Further, level sensor detection mode is photosignal detection, current type or condenser type；Photosignal detectsCleaning Principle be that to produce absorbance difference at pipeline obvious due to liquid and air.
Further, this analyser also includes a sample pre-dilution dish, pre-dilution before sample detection.
The present invention also provides for a kind of biochemical detection system based on above-mentioned automatic clinical chemistry analyzer, and this system includes multipleDescribed biochemistry analyzer, main control computer, sample treatment unit, sample transfer unit 15 and sample collection unit.Described each biochemistryAnalyser has been used for disparity items detection；Described main control computer is used for controlling the work of each functional unit；Described sample treatment listUnit is used for centrifugal treating testing sample, automatically opens up sample lid and put the most successively to sample transfer unit；Described sampleTransfer unit 15 is for transmitting sample between biochemistry analyzer；Described sample collection unit is used for collecting sample after detection.ShouldDifference reagent detection project is completed by system respectively by different biochemical instruments, so can reduce separate unit biochemical instruments reagent unit and loadAmount, and reduce varieties of reagent intersection, reduce the cross-contamination to detection cup of the different reagent.
Below as a example by two reagent set and two mixing needles, the workflow of automatic clinical chemistry analyzer of the present invention is describedJourney:
Step 1: when the detection cup 14 of cleaning rotates to the first reagent adding position, a reagent unit in the first reagent set 2Reagent one is added in this detection cup 14；
Step 2: reaction tray 1 rotates a circle when stopping, detecting cup 14 and crosses former reagent adding position, be arranged in it in step 1After one detection cup stay in former reagent adding position, identical or another reagent unit of the first reagent set backward one detection cup addEnter reagent one；
Step 3: reaction tray 1 often rotates a circle, respectively detection cup is along the position of reaction tray direction of rotation one cup of advance, whenDetection cup 14 arrives when adding sample position, and sample pin 6 adds quantitative sample in detection cup 14；
Step 4: reaction tray 1 continues each detection cup position along reaction tray direction of rotation one cup of advance that often rotates a circlePutting, when detecting cup 14 and arriving the first mixing position, liquid in detection cup is stirred by the first mixing needle 5；
Step 5: reaction tray 1 continues each detection cup position along reaction tray direction of rotation one cup of advance that often rotates a circlePutting, when detecting cup 14 arrival and adding the second reagent adding position, the second reagent set 4 adds reagent two；
Step 6: after adding reagent two, detection cup 14 moves to the second stirring position, and the second mixing needle 19 is in detection cup 14Liquid is stirred；
Step 7: reaction tray 1 drives the detection circulating rotation of cup, through one-time detection point in each rotary course, to detectionIn cup, liquid carries out an optical signal detecting；
Step 8: reaction tray 1 drives the detection circulating rotation of cup, respectively detects cup by detecting cup cleaning reaching cleaning positionDevice 3 cleans one by one；
Step 9: after completing to clean, reaction tray 1 drives the detection circulating rotation of cup, and detection cup clean after cleaning revolves againGo to the first reagent position.
Step 10: the detection cup after cleaning continues according to step 1-9, has circulated new pattern detection.
The invention will be further described with embodiment below in conjunction with the accompanying drawings.
Embodiment 1
In the present embodiment, reagent set is 2 groups, the respectively first reagent set 2 and the second reagent set 4, and mixing needle quantity is 2,Being respectively the first mixing needle 5 and the second mixing needle 19, the first mixing needle 5 and the second mixing needle 19 are arranged on reaction tray 1 not coordinationPut, liquid in stirring and evenly mixing detection cup.
As shown in figures 1 to 6, in the first reagent set 2 the reagent pin reagent exit of a reagent unit by shared reagentPin mobile device moves to detect cup position, and the reagent transfer device of reagent unit draws reagent one, and drives quantitative reagentIn the outlet of reagent pin joins a detection cup, reaction tray is through multiple rotary afterwards, and this detection cup 14 stops at sample bitBeing drawn testing sample by sample needle 6 specimen cup from circular sample dish 8 when putting and add in above-mentioned detection cup 14, detection cup 14 is through continuingContinuous be rotationally advancing stir to the first mixing position by the first mixing needle 5 after, detection cup 14 continues to be rotationally advancing to the second reagent set 4During reagent adding position, the shared reagent pin mobile device of the second reagent set 4 is by mobile to this detection cup position for a reagent pin outletPutting, the reagent transfer device in the second reagent set 4 drives quantitative reagent two to join above-mentioned detection cup 14, and detection cup 14 continuesRotating after stirring to the second mixing position by the second mixing needle 19, detection cup 14 gradually detects, through tool count in rotary courseObtaining testing result according to processing, detection is cleaned device 3 by detection cup after terminating and is carried out detection cup 14.
Embodiment 2
As in figure 2 it is shown, the difference of the present embodiment and embodiment 1 is, specimen cup is loaded on rail mounted specimen holder 7, railRoad formula specimen holder 7 straight line single-way moving along its length, each sample is through sample position, and sample is got inspection by sample pinSurvey in cup 14 and perform detection.
Embodiment 3
In conjunction with Fig. 1 and Fig. 2, the present embodiment mesophytization analyser detects multiple different biochemical project simultaneously, but in reaction tray 1Each detection cup only cycle detection identical items, and do not detect other project.
In the present embodiment, reaction tray 1 designs and only loads 40 detection cups, 20 biochemical projects of cycle detection, detection project wheelTurning number of times is 2.Respectively detect in cup at 1-20 respectively according to same sequence every time and add a kind of reagent in the first reagent set 2, afterwardsAdd the first sample, stir, then according to same sequence is at 1-with the first mixing needle 5 when reaction tray rotates to the first stirring position20 each detection cups add a kind of reagent in the second reagent set 4 every time, stirs with second when reaction tray rotation to the second stirring positionMix pin 19 to stir.No. 21-40 detection cup adds a kind of reagent in the first reagent set 2 one by one according to the order of 1-20 before subsequently,Add the second sample afterwards, stir with the first mixing needle 5 when reaction tray rotates to the first stirring position, then according to same sequenceIn No. 21-40 detection cup, add a kind of reagent in the second reagent set 4, stir with second when reaction tray rotates to the second stirring positionMix pin 19 to stir.So can realize each detection cup and only detect identical items, and not detect disparity items.The most each detection cup is onlyOnly accept identical reagent, and sample is different.
Embodiment 4
As it is shown on figure 3, the present embodiment is by vacuum pump the 9, first pressure regulation bottle 10 and the second pressure regulation bottle 11Realize detection cup is cleaned suction and the supply of air pressure mixing needle of device 3 waste liquid.
Its concrete structure and operation be: closes the first valve V1 and the 3rd valve V3, and vacuum pump 9 is to the first pressure regulation bottle10 bleed formation negative pressure；Now open the first valve V1, owing to being negative pressure in the first pressure regulation bottle 10, detect the waste liquid in cupIt is inhaled in the first pressure regulation bottle 10.While vacuum pump 9 work, the second pressure regulation bottle 11 is blasted air and make this bottleThe malleation that middle formation is higher, opens the second valve V2, and gas is sprayed by blender gas pressing 5, local, impulse detection cup surface, rightIn detection cup, liquid realizes noncontact stirring.When second pressure regulate bottle 11 air pressure too high time can by open the 4th valve V4,Suitably release portion gas, keep the second pressure regulation bottle 11 air pressure moderate.
When the waste liquid that the first pressure regulates bottle 10 runs up to a certain amount of, vacuum pump 9 break-off, the first valve V1 and theThree valve V3 open, and the waste liquid accumulated in the first pressure regulation bottle 10 can be by being automatically drained out inflow waste collection in bottle due to gravityIn device 12.
Embodiment 5
In conjunction with Fig. 4 (a), described contactless air pressure mixing needle 5 quantity is 1, is arranged on detection cup 14 ullage oneSide, the first barostat 5 forms impact air pressure in detection cup at liquid level 20 local, and in making detection cup, liquid level 20 local pressure is more thanOther parts so that declined by high-pressure section liquid level, localized liquid flows to area of low pressure, and relatively low pressure part liquid level due toOther region liquid extrudes and rises so that the liquid in cup flows.Fig. 4 (b) display stops pressure when air pressure mixing needle,Former local is reduced by high-pressure section pressure and recovers normal, and liquid refluxes, and Fig. 4 (c) is build-up of fluid normal position in detection cupView.
Embodiment 6
As shown in Figure 5 and Figure 6, the most neat row of the reagent exit of multiple reagent pin N of each reagent unit in the present embodimentRow, according to detection needs, by driving the cooperation of motor M1 and driven pulley M2 to drive belt to turn in shared reagent pin mobile device MDynamic, control reagent pin and move, the outlet of difference reagent pin is moved on to detect respectively cup position, then corresponding reagent unit automatically will be correspondingQuantitative reagent joins in detection cup, performs different project detections.
Embodiment 7
As shown in Figure 6, the present embodiment is provided with reagent catcher 13, is used for when start or after the reagent that changes the outfit by reagentTransfer device 18 extracts a small amount of reagent and is drained into reagent catcher 13, it is ensured that when instrument works, reagent pin mouth delivered to by reagentPlace, the reagent adding detection cup each time is enough.
Embodiment 8
As it is shown in fig. 7, in the present embodiment reagent unit include reagent bottle 16, level sensor 17, reagent transfer device 18,5th valve V5, the 6th valve V6 and the first reagent pin N.Reagent transfer device 18 uses syringe, when needs distribution reagentBeing first turned on the 5th valve V5, close the 6th valve V6, syringe piston is drop-down, during in reagent bottle 16, reagent enters syringe；Closing the 5th valve V5 afterwards, open the 6th valve V6, syringe piston pushes away, reagent is flowed out by the first reagent pin N.
Embodiment 9
As shown in Figure 8, unit based on the present embodiment biochemistry analyzer based on the present invention, combination constitutes a kind of high efficiencyStreamline biochemical detection system, this system is single by main control computer, multiple stage biochemistry analyzer, sample treatment unit, sample transmissionUnit 15 and sample collection unit composition.Main control computer is for controlling the co-ordination of each functional unit, and each sample is in differenceBiochemistry analyzer carries out project detection, and testing result concentration is pooled to main control computer and is analyzed and reports.Each biochemistry analyzerFor completing disparity items detection, sample only completes some projects detection, multiple stage through each biochemical instruments in this biochemical instrumentsInstrument completes sample the required entries purpose detection jointly；Or every instrument completes the detection of the whole project of each sample.SampleProduct processing unit is used for centrifugal treating testing sample, automatically opens up sample lid and put the most successively to sample transfer unit.Sample transfer unit 15 is for transmitting sample between biochemistry analyzer.Described sample collection unit is used for collecting sample after detectionProduct.
Embodiment 10
As it is shown in figure 9, the difference of the present embodiment and embodiment 1 is that analyser also includes reagent disc 22 and the second reagent pin21；Drawing reagent one by the first reagent set 2, second reagent use reagent disc 22 and the mode of the second reagent pin 21 structural groupingDraw.