Modes for carrying out the inventionIn order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or intervening elements may also be present.
It should be noted that the terms of orientation such as left, right, up, down, etc. in the present embodiment are only relative concepts or reference to the normal use state of the product, and should not be considered as limiting.
As shown in fig. 1 to 6, the smear preparation apparatus provided by the embodiment of the present invention can be used for smear preparation of samples such as blood and body fluid. The smear preparation device comprises a sampling module 1 for extracting a sample, a slide loading module 3 for moving a slide to a working line, a printing module 4 for printing information on the slide, a sample dripping module 5 for dripping the sample on the slide, a slide pushing module 6 for flatting the sample on the slide, an overturning module 64 (not shown in the figure) for overturning the slide and a dyeing module 9 for dyeing the slide, wherein the slide loading module 3, the printing module 4, the sample dripping module 5, the slide pushing module 6, the overturning module 64 and the dyeing module 9 are arranged along the working line, the smear preparation device is compact in structure, the smear preparation efficiency is high, the smear preparation reliability is high, and the accuracy of a diagnosis result is guaranteed. Stations are distributed on the working line, the sampling station is arranged at the position of the sampling module 1, the slide loading station is arranged at the position of the slide loading module 3, the printing station is arranged at the position of the printing module 4, the blood dripping station is arranged at the position of the sample dripping module 5, the push piece station is arranged at the position of the push piece module 6, the overturning station is arranged at the position of the overturning module 64, and the dyeing station is arranged at the position of the dyeing module 9. It should be noted that, the glass slide in this embodiment may be made of other suitable materials instead of glass, and all of them fall within the protection scope of the present invention.
In particular, the smear preparation apparatus further comprises a cleaning module (not shown in the figure) for cleaning the slide, wherein the cleaning module is arranged along the working line, and the cleaning module is a cleaning station. In the step before the dropping module 5 performs the dropping of blood, for example, the slide may be cleaned by a cleaning module before the sample information is printed, for example, the slide may be cleaned by blowing air, and the like.
Specifically, as shown in fig. 1 to 3, the smear preparation apparatus further includes a sample drying module 8 for drying the sample on the slide, and the sample drying module 8 can be used for drying the sample such as blood film. The sample drying module 8 is arranged along the working line, and the sample drying module 8 is a slide drying station, so that a blood sample is dried in time.
Specifically, as shown in fig. 1 to fig. 3, the smear preparation apparatus further includes a slide output module 10 for carrying a slide basket and outputting the slide basket to a set position, where the slide output module 10 is a slide output station.
Specifically, as shown in fig. 1 to 3, the smear preparation apparatus further includes a slide basket drying module for drying the stained slide, and the slide basket drying module 12 is a slide basket drying station.
Specifically, as shown in fig. 1 to 3, the dropping sample module 5 includes a blood dropping needle 501, and the blood dropping needle 501 is connected to the sampling module 1; one side of the blood dropping needle 501 is provided with a micro sample injection module 2 for moving a preferential sample to the lower part of the blood dropping needle 501. The direct direction of the test tube that drops into needle 501 is removed to the test tube that microsample module 2 can put into operating personnel, and simultaneously, needle 501 also can remove to the direction of the test tube that operating personnel put into, owing to need not to extract blood through sampling module 1, can reduce the demand of blood sample to realize the trace and advance the kind preferentially. Further, the same blood drop needle 501 may be used for batch sampling and micro-and preferential sampling.
Specifically, as shown in fig. 1 to 3, the blood dropping needle 501 includes a needle body and a guide sleeve, and an elastic structure is disposed between the needle body and the guide sleeve to prevent the blood dropping needle 501 from rigidly impacting a slide.
Specifically, as shown in fig. 1 to 6, the working lines include a first working line 101 and a second working line 102; the slide loading module 3, the cleaning module, the printing module 4, the dripping module 5, the pushing piece module 6 and the overturning module 64 are arranged along the first working line 101, and the overturning module 64 is positioned at the joint of the first working line 101 and the second working line 102; the sample drying module 8 and the staining module 9 are arranged along the second working line 102, and the first working line 101 and the second working line 102 are arranged in a perpendicular intersecting manner or in a straight line or in other suitable angles.
Specifically, as shown in fig. 1 to 11, the first working line 101 is provided with a first linear slide rail 111, and a slide pushing cart for carrying a slide is slidably connected to the first linear slide rail 111; the second working line 102 is provided with a second linear slide rail 112, and a manipulator module 113 for clamping and moving the slide is connected on the second linear slide rail 112 in a sliding manner; the first working line 101 and the second working line 102 are perpendicularly intersected or linearly connected. For example, in this embodiment, the first work line 101 and the second work line 102 are arranged in a "T" shape, the turnover module 64 is located at the joint of the first work line 101 and the second work line 102, wherein the sample drying module 8 and the staining module 9 are respectively located at the left and right sides of the turnover module 64, and the slide loading module 3, the cleaning module, the printing module 4, the dropping sample module 5 and the slide pushing module 6 are located at the same side of the turnover module 64.
Specifically, as shown in fig. 1 to 11, the smear preparation apparatus further includes a sample automatic sampling module 114, the sample automatic sampling module 114 is disposed adjacent to the sampling module 1, and the sample automatic sampling module 114 can deliver the tube rack to the sampling module 1; the sample autoinjection module 114 comprises a test tube rack feeding part and a sample mixing part.
Specifically, as shown in fig. 1 to 11, the slide loading module 3 is a slide storing and distributing module, which includes a slide storing submodule 31 for accommodating slides, a cutter head submodule 33 for pushing slides from the slide storing submodule 31 to a working line, and an in-position blocking submodule 32 for limiting the slides or the cutter head submodule 33.
Specifically, as shown in fig. 1 to 11, the slide module 6 includes a slide cutter head sub-module 61 for smoothing the sample, and an inversion module 64 for inverting the slide from flat to upright.
Specifically, as shown in fig. 1 to 11, the smear preparation apparatus has a control part for controlling a robot to grip and move a slide manually inserted at the specimen drying module 8 to the staining module 9. When only the slide on which the blood sample has been dropped needs to be stained, the slide on which the blood sample has been dropped can be directly placed at the sample drying module 8, and the manipulator can be made to grip the slide at the sample drying module 8 by the control section and feed the slide to the staining module 9. The control means may be a button or the like. The sample drying module 8 has a first receiving groove 81 and a second receiving groove 82 to receive a slide coated with a sample, and a drying air flow can flow out inside both the first receiving groove 81 and the second receiving groove 82 to dry the sample. Wherein the second receiving groove 82 can be used for placing the manually inserted slide, and the manipulator module 113 can clamp the slide in the second receiving groove 82 and place the slide in the staining station through the control part. The first receiving groove 81 and the second receiving groove 82 correspond to the first drying station and the second drying station, respectively.
Specifically, as shown in fig. 1 to 11, the dyeing module 9 includes a turntable submodule, a dial submodule and a dyeing box; the staining module 9 has a slide entrance station 901 (staining station 1) and a slide removal station 902 (staining station 4), and the slide entrance station 901, the slide removal station 902, the flip module 64, the sample drying module 8, and the slide output module 10 are arranged along the same straight line, so that a manipulator capable of moving linearly can move slides to the respective stations.
Specifically, as shown in fig. 1 to 11, the slide basket drying module 12 includes a housing 120, and the housing 120 is provided with an air blowing port 1201 for blowing out an air flow and an air suction port 1202 for sucking in the air flow, so that a better convection effect can be achieved, and the slide in the slide basket 11 can be dried quickly. The housing 120 may have two air flow passages therein, i.e., an air outlet passage and an air inlet passage. An air outlet fan can be arranged at the air outlet channel, and an air suction fan can be arranged at the air suction channel. Alternatively, the casing 120 may have only one circulation channel, the two ends of the circulation channel are the air blowing port 1201 and the air suction port 1202 respectively, and a circulation fan may be disposed in the circulation channel or/and at the air blowing port 1201 or/and the air suction port 1202.
In this embodiment, as shown in fig. 1 to 11, all the working positions may be designed as two perpendicularly intersecting working lines, including: a first working line 101 (smear working line) from the right side to the left side of the smear preparation apparatus, and a second working line 102 (dyeing and output working line) perpendicularly intersecting the smear working line. Loading, printing, blood dripping, push sheet and turning working positions are distributed on the push sheet working line from left to right, and slide output, blood film drying, blood film development detection, turning, dyeing station 1 (placing slide in) and dyeing station 4 (taking slide out) are distributed on the dyeing and output working line from front to back. These 2 station lines intersect at the inverting station, and switching of the slide placing state (from horizontal to vertical) is completed at the inverting station. The layout can ensure that the whole machine can ensure that the slide is always in an accurate position control state on the premise of meeting the realization of various functions, reduce the clamping times of the slide to the maximum extent and solve the card fault of the slide in the operation process of the instrument. Meanwhile, the stations are designed on two concentrated station lines, so that the device plays an important role in reducing the volume of the whole machine.
In the design of the working line, considering that the printing is easily influenced by the quality of an external input slide and has more faults, the blood dripping station design of the sample is arranged behind the printing station. The scheme can effectively avoid the situation that the sample is wasted due to small printing faults, so that the sample collection and the blood dripping are required to be carried out again, and particularly for a small amount of samples, the design is necessary. Meanwhile, in order to reduce the influence of the foreign matters on the surface of the slide, which are free, on the printing quality, a special cleaning station is designed between the slide storage and distribution module and the slide information printing module 4. The special cleaning module is adopted to clean the free foreign matters on the surface of the slide, so that the free foreign matters on the surface do not exist in the printing area when the slide is printed, and the faults caused by the influence of the surface quality of the slide on the printing are reduced to the maximum extent.
In this embodiment, the sample automatic sampling module 114 mainly includes a sampling sub-module and a sampling sub-module, and can automatically collect and process samples of closed test tubes continuously or discontinuously arranged on the test tube rack. The sampling submodule and the sampling submodule are relatively independent in structure, and can be conveniently mounted and dismounted according to different application environments (such as a single full-automatic blood smear preparation instrument or a full-automatic blood smear preparation instrument on a certain full-automatic production line), without influencing the functional integrity of the sampling submodule. The full-automatic blood smear preparation instrument can be rapidly configured in different scenes, and the independent or high-speed and automatic rechecking function can be realized according to the requirement.
The micro sample injection module 2 can also be used as an open sample injection module, when the sample amount is small, or after the push sheet is started, a certain sample needs to be processed by a priority queue, and then the micro sample injection module 2 can be used. The micro sample introduction module 2 can automatically collect and process a single sample put into the test tube adapter. Which may include sample feed, sample unload functions. In the micro sample introduction module 2, the sampling needle used for sample collection and the blood dropping needle 501 used by the sample dropping module 5 adopt the same needle, so that the requirement on the sample amount can be reduced to the greatest extent; meanwhile, the same needle is used, so that the sample transportation distance is short, and the deformation influence of the transportation process of the sample in the instrument on the tangible cells of the sample can be effectively reduced.
As shown in fig. 1 to 4, the slide loading module 3 (slide storing and distributing module) mainly comprises a slide cassette sub-module 31, a cutter head sub-module 33, and an in-place blocking sub-module 32, and can load the slides in a horizontal state into a stack by a user, and the slide storing and distributing module automatically loads the slides one by one to the slide pushing trolley of the slide pushing module 6 according to the sample requirement. Cassettes may be designed with relatively independent structures, but are different from readily removable structures. In normal operation, the cassette is locked by a special locking mechanism. Through locking, the accuracy of the relative position of the slide box and the slide distribution guide rail can be reliably ensured, and the slide box with an easily detachable structure is prevented from shaking due to external interference, so that slide distribution failure is avoided. In the process of fault resolution and instrument maintenance, an operator can conveniently detach the slide box by opening a special locking mechanism, so that the aim of independent detachment is fulfilled.
The printing module 4 can print the information (which may be the number, time, type, etc.) preset by the user on the frosted area of the slide by using the thermal transfer method, so as to identify the slide. The ink ribbon and the slide can be stationary, and the printing head can move relative to the ink ribbon, so that the ink ribbon and the slide are absolutely stationary, and the ink ribbon is prevented from being broken due to the movement of the ink ribbon and the slide during printing to the greatest extent.
The dripping module 5 is mainly divided into a sample sucking submodule and a blood dripping submodule. The blood dropping needle 501 has a structure with an elastic guide sleeve. When the blood dropping needle 501 moves downwards and touches a slide, the blood dropping needle 501 is limited to move downwards and stop, and the fixing support of the blood dropping needle 501 is allowed to move downwards for a small buffer distance due to the elastic structural design of the guide sleeve. Therefore, the blood dropping needle 501 can be used for dropping blood without accurate position control, namely, the bottom end surface of the blood dropping needle 501 can be always contacted with the surface of the slide, and the consistency of the blood drops on the surface shape of the slide is realized.
As shown in fig. 1 to 11, the push sheet module 6 is mainly divided into a push sheet trolley sub-module 62, a push sheet cutter head sub-module 61, a blade cleaning sub-module 63 and a turning module 64. The method comprises the following steps: the slide clamping, the slide pushing parameter control, the slide unloading, the slide overturning and the blade cleaning functions. The slide pushing trolley submodule 62 realizes the fixing and loosening of the slide on the trolley by using the power of the movement of the slide pushing trolley submodule. And 5 actions of loading, printing, dripping blood, pushing and overturning the slide are finished through one-time clamping, so that the slide is effectively prevented from being damaged, and faults in the slide transportation process are reduced. The turning module 64 adopts a single-piece turning structure, so that the structure is simple and the turning is reliable. In the process of turning, the slide is firstly turned at a certain small angle and then turned to a vertical position after waiting for the preset time of the system. Therefore, the problem that blood trickling occurs at the blood dripping position of the blood membrane due to the fact that the blood membrane is too thick after the push piece is completed can be effectively solved.
As shown in fig. 1 to 11, the manipulator module 113 serves as a main carrying mechanism of the staining and discharging line, and realizes the transfer of the slide between the slide discharging, the blood film drying, the blood film developing and detecting, the turning, the staining station 1 (slide loading) and the staining station 4 (slide unloading) in the vertical placement state. Six stations, namely a slide output station, a slide drying station (blood film drying station), a slide pushing station (blood film unfolding detection station), a turning station, a dyeing station 4 and a dyeing station 1, are linearly distributed, so that the manipulator can simply complete all station actions in the linear direction. The sample drying module 8 can also be called a blood film drying module to realize the function of drying the blood film of the slide finished by the push piece, and meanwhile, an interface is provided as an unstained slide input from the outside in the interface of the whole machine. When a user needs to perform single-staining scenes, the slide finished by the push piece is manually placed into the blood film drying module. The sample drying module 8 is used as a blood film drying module in the whole structure and also used as an interface for an external input unstained slide, so that the whole structure is simplified and the simplification of the whole structure is realized on the premise of not reducing the functions of instruments. Meanwhile, the module controls three factors of the relative humidity, the drying temperature and the flow rate of the drying medium through design, so that the drying speed is greatly increased, and the problem that the blood membrane cannot be thoroughly dried due to the fact that other products in the market are low in environment temperature and air humidity is thoroughly solved. Moreover, the module provides a blowing drying method for the drying air flow to flow along the sample (blood membrane) development direction, so that the cell morphology of the blood membrane can be ensured not to change under the drying of micro air flow, and the problem that the cell deformation caused by other products in the air drying process gives wrong diagnosis conclusion is solved.
The staining module 9 stains the slide with the slide pushing completed according to a staining procedure pre-selected by the user. The method mainly comprises the following steps: a turntable submodule, a dial submodule and blocking submodule 32 and a dyeing box. The turntable submodule and the dyeing box provide places required by the slide to be dyed and complete the dyeing process. The needle dial submodule bears all the liquid adding and discharging needles, and all the needles are inserted into or lifted from the dyeing box along with the up-and-down movement of the needle dial, so that the liquid adding and discharging of the dye liquor are realized. In the dyeing module 9, at least one or more blocking sub-modules 32 are provided, and the blocking sub-modules 32 realize the switching of the dyeing box from the previous dyeing station to the next dyeing station. The dyeing module 9 adopts a disc structure in structure, and the structure is small. The needle dial is adopted to be integrally up and down together with all the liquid adding needles and the liquid discharging needles, so that the problem that the liquid adding needles and the liquid discharging needles of all dyeing stations need one power mechanism is solved. The dyeing box transfers along with the turntable by utilizing the friction force between the dyeing box and the turntable on the turntable, and the stopping mechanism is used for controlling the time point of the transfer of the dyeing box, so that the user-defined dyeing process is realized.
As shown in fig. 1-11, the slide output module 10 is primarily a slide basket loading sub-module 115, a slide basket feeding sub-module 116, a slide drying sub-module (slide basket drying module 12), and a slide basket unloading sub-module 117, including loading, slide basket transport, slide storage, and slide basket drying functions. The slide basket loading submodule 115 can be designed by adopting a double-belt feeding mechanism, can accurately detect and display the number of available slide baskets in the instrument, can continuously load the slide baskets at any time, and can give an alarm when the number is insufficient, thereby meeting the requirement of a user for adding the slide baskets under any condition. The application of the freely telescopic pusher dog mechanism realizes that the number of the empty slide baskets is increased by 50 percent on the premise of not occupying much space. The use of a slide basket pinch roller mechanism in the slide basket feed sub-module 116 ensures the motion accuracy of the slide basket and improves the reliability of slide collection. The slide basket drying module 12 dries the slide by blowing hot air, evaporates water on the surface of the slide by heating, and takes away moisture by flowing air to achieve a drying effect. The air blowing port 1201 has a large area, and can place a plurality of slides at a time, providing work efficiency. Meanwhile, the slide basket drying module 12 is designed with an air blowing port 1201 and an air suction port 1202 such that the slide basket channel is located between the air ducts of the air blowing port 1201 and the air suction port 1202. When the slide basket loaded with the slide reaches a designated drying position, the slide basket drying module 12 blows strong wind with constant temperature and constant speed from the air blowing port 1201, and simultaneously, a fan with flow rate much larger than that of the air blowing port 1201 is started from the air suction port 1202 to suck air, so that effective convection is formed at the slide basket. Meanwhile, the design of hollowing out the periphery of the glass slide basket is matched, and the drying time is greatly reduced under the double-layer influence of temperature and air convection.
As shown in fig. 1 and 10, the present embodiment also provides a slide basket drying module 12 including a housing 120, the housing 120 being provided with an air blowing port 1201 for blowing out an air flow and an air suction port 1202 for sucking in the air flow. Thus, a better convection effect can be formed, and the slide in the slide basket is favorably and quickly dried. The air blowing port 1201 may be directed toward the top of the slide basket and the air suction port 1202 may be directed toward the side wall of the slide basket. The housing 120 may have two air flow passages therein, i.e., an air outlet passage and an air inlet passage. At least one air outlet fan can be arranged at the air outlet channel, and at least one air suction fan can be arranged at the air suction channel. Alternatively, the casing 120 may have only one circulation channel, the two ends of the circulation channel are the air blowing port 1201 and the air suction port 1202 respectively, and a circulation fan may be disposed in the circulation channel or/and at the air blowing port 1201 or/and the air suction port 1202. The slide basket drying module 12 may have slide basket channels just between the air plenum of the air blowing port 1201 and the air plenum of the air suction port 1202. When the slide basket loaded with the slide reaches a designated drying station, the slide basket drying module 12 blows strong wind with constant temperature and constant speed from the air blowing port 1201, and meanwhile, a fan with flow rate far greater than that of the air blowing port 1201 is started from the air suction port 1202 to suck air, so that effective convection is formed at the slide basket. Meanwhile, the design of hollowing out the periphery of the glass slide basket is matched, and the drying time is greatly reduced under the double-layer influence of temperature and air convection.
As shown in fig. 1 to 11, the embodiment of the present invention further provides a smear preparation method, which can apply the above smear preparation apparatus, the smear preparation method including the steps of setting a working line in which the sampling module 1, the slide loading module 3, the printing module 4, the dropping sample module 5, the push piece module 6, the turnover module 64, and the staining module 9 are arranged, laying a slide flat and moving the slide along the working line, in the process that the slide moves along the working line, the slide is cleaned through the cleaning module, then sample information is printed on the slide through the printing module 4, then a sample collected from the sampling module 1 is dripped on the slide through the sample dripping module 5, the sample of the slide is smoothed through the slide pushing module 6, the slide is turned from a flat state to an upright state through the turning module 64, and the turned slide is dyed through the dyeing module 9.
Specifically, in the process of turning the slide from the flat state to the upright state, the turning module 64 turns the slide to be inclined and stays for a set time period, so that the sample is dried to a certain degree and then is turned to the upright state, thereby ensuring the diagnosis accuracy.
Specifically, after a sample is printed on the slide and before the sample is dropped on the slide, the sample information printed on the slide is checked by a detection module.
In a specific application, the front end of the smear preparation apparatus may be a sample autosampler module 114, where a user puts in a sample to be tested, or other workstation apparatus (such as a cascade pipeline) automatically transfers the sample in. The module completes the functions of test tube sample introduction, test tube presence or absence detection, bar code scanning, sample mixing, puncture sample suction and the like. For special samples (such as body fluid and the like) or uncapped samples, the micro/open sample introduction module can be used for absorbing samples, and the push piece dyeing can be ensured under the condition of low sample amount. Meanwhile, the sample automatic sampling module 114 and the micro/open sampling module respectively transmit the collected sample information to the sample dropping module 5 for processing, so as to extract the sample characteristics related to the push sheet for the subsequent push sheet.
The right side of the smear preparation device can be provided with a slide storage and distribution module, a user loads a pile of slides in a horizontal state, the slide storage and distribution module automatically loads the slides one by one to a slide pushing trolley of the slide pushing module 6 according to the sample requirement and fixes the slides on the trolley in a horizontal state. In the slide storage and distribution module, a special slide cleaning station is designed, and the special cleaning module is adopted to clean the free foreign matters on the surface of the slide, so that the free foreign matters on the surface do not exist in a printing area when the slide is printed, and the fault caused by the influence of the surface quality of the slide on the printing is reduced to the maximum extent. Then the slide moves on the slide pushing trolley of the slide pushing module 6 together with the trolley to the left side of the smear preparation device step by step. The trolley in the embodiment firstly reaches a printing position, and the sample information is printed by matching with the slide information printing module 4; after the printing action is confirmed to be successfully completed, the blood dropping station is reached; dropping the sample collected by the sample automatic sampling module 114 or the micro/open sampling module on the slide by matching with the dropping sample module 5; when the slide reaches the slide pushing position, intelligently adjusting slide pushing parameters according to the characteristics of the sample extracted by the sample dripping module 5, so that the sample is smeared on the slide in the optimal state, and the slide pushing action is completed; then, the slide is finally turned from the horizontal state to the vertical state by the turning module 64 of the pusher module 6.
The manipulator module 113 on the left side of the smear preparation device penetrates through the front and back direction of the whole instrument and is connected with the turnover position, the blood film drying position, the dyeing station 1, the dyeing station 4 and the slide output position of the slide in a vertical state. The manipulator module 113 transfers the slide on which the slide pushing is finished to the blood film drying module at the turning position of the slide pushing module 6, and the sample is fully dried in the blood film drying module, so that the blood film can not fall off in the subsequent dyeing process. After a fixed time, the robot module 113 transfers the slides with the samples dried to the staining cassettes at station 1 of the staining module 9, one for each slide. The slide is dyed according to a dyeing flow set by a user (the dyeing flow can be customized by the user in a certain range) in the dyeing box, and the instrument automatically sucks and arranges dye liquor in each dyeing box, so that the slide dyeing is realized. The stained slide with the staining cassette will be transferred from staining station 1 to staining station 4 within staining module 9. From the staining station 4, the robot module 113 takes the stained slide out of the staining cassette, which automatically enters the next staining cycle.
The manipulator module 113 transfers the slides from the staining station 4 of the staining module 9 to the slide basket 11 with sealed bottom and hollowed periphery on the slide output module 10, and the slides are arranged one by one with gaps. The slide output module 10 counts the slides loaded in the slide basket, when the slide basket 11 is full or the slide pushing and dyeing process is completed, the slide output module 10 transports the slide basket 11 to a drying mechanism of a slide basket drying module 12 of the slide output module 10, and the drying mechanism blows out strong wind with constant temperature and constant speed to dry the dyed slides. After a fixed period of forced air drying, all slides were fully dried. The slide output module 10 pushes the slide along with the slide basket 11 onto the unloading platform of the slide output module 10, at which point the entire slide pushing and staining procedure is completed.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting, and any modifications, equivalents or improvements made within the spirit and principles of the present invention, such as adjustment of the sequence of the stations in each work line, or combination or decomposition of the number of stations, and adjustment of the flow correspondingly, should be included in the scope of the present invention.