Liquid-based cell sample manufacturing equipment, method and the film cylinder for liquid-based cell sample manufacturingTechnical field
The present invention relates to medical instruments field, in particular to a kind of liquid-based cell sample manufacturing equipment, method and the film cylinder for liquid-based cell sample manufacturing.
Background technology
Cervical exfoliated cell diagnosing cervical is utilized to originate from the forties in last century, its tabletting technology has been used up traditional manual smearing method, slice-making quality is completely by the film-making experience influence of operator, the problem of most critical is that the sample taken out cannot repeat film-making and the smear cells made seriously is piled up, and identifies difficulty；Until the nineties in last century occur in that a kind of new flaking method, i.e. liquid-based cell sample manufacturing art, so far the mankind just can utilize this technology in strange land, repeatable, make clearly thin smear for doctor diagnosed.
Liquid-based cell sample manufacturing currently mainly has three kinds of methods: natural sedimentation method, membrane type suction filtration and direct centrifuging.Three kinds of method film-makings are respectively arranged with it shortcoming.Natural sedimentation method is to utilize centrifuge by centrifugal for sample layering, takes out useful layer liquid, is placed on microscope slide, sinks on microscope slide by gravity；Membrane type suction filtration is to make use of microporous membrane technology to complete film-making under vacuum aided；Directly centrifugal rule have employed centrifuge and directly utilizes centrifugal force and be attached to heavy for sample on microscope slide.
This wherein membrane type suction filtration method of tableting be main flow method of tableting.
Existing membrane type suction filtration mainly has two classes, and one is absorption method, and one is Filtration.
Absorption method is the lower section that sample is in film, when film contact liq, under vacuum, it is sucked away less than the material of fenestra, is blocked and is attached to more than the cell of fenestra on film, film is moved on microscope slide, form normal pressure air pressure in the direction deviating from microscope slide of film, cell is blown on microscope slide.In principle, having how many fenestras just can adsorb how many cells, if fenestra is evenly distributed, therefore cell distribution is also uniform, and the cell sheet now made is clearly uniform.The shortcoming of this method relates to air pressure change, and not only mechanism is complicated, and film-making speed is slow, be typically in 3 minutes a piece of, in addition that the requirement of sample liquid is also higher, if sample liquid early stage process bad, causing that large-area material is adsorbed on fenestra, the slice, thin piece made there will be clear area.
Filter rule is the top that sample is in film, it is generally bimodulus to filter, first tunic is coarse filtration, filter impurity and the mucus of large volume, the second tunic fine filtering, under vacuum, it is sucked away less than the material of fenestra, being blocked on fenestra more than the cell of fenestra, machine will be left in the cell on fine filtering film and is transferred on microscope slide, completes film-making.Sample liquid is also had certain requirement by the method, if sample liquid is not overrich, application of sample amount is suitable, the slice, thin piece made is equally clear, but uniformity is good not as absorption method, therefore the method need operator rule of thumb to judge application of sample amount is how many, otherwise of low quality in flakes, in addition film-making process needs to disassemble film device, complicated operation.
Summary of the invention
A kind of liquid-based cell sample manufacturing equipment of offer, method and the film cylinder for liquid-based cell sample manufacturing are provided, complicated to solve membrane type suction filtration tableting operation of the prior art, inefficient problem.
To achieve these goals, according to an aspect of the invention, it is provided a kind of liquid-based cell sample manufacturing equipment, including: lifting arm；Film-making portion, is arranged on lifting arm, and film-making portion includes the film cylinder connecting portion arranged along the direction of motion of lifting arm；Moving station portion, including one or more station groups, each station group all includes the specimen holder of the sample liquid for placing liquid basal cell and for placing the microscope slide seat of microscope slide, moving station portion can move and make specimen holder move with microscope slide seat to the operating position corresponding with film-making portion, when specimen holder and microscope slide seat are positioned at operating position, specimen holder and microscope slide seat are on the motion path of film cylinder connecting portion or the extended line of motion path.
Further, station group also includes the film cylinder base for placing film cylinder, and moving station portion can move and make film cylinder base move to the operating position corresponding with film-making portion, and when film cylinder base is positioned at operating position, film cylinder base is on the motion path of film cylinder connecting portion.
Further, in station group, specimen holder is arranged between film cylinder base and microscope slide seat.
Further, moving station portion is in disk form, and the direction of motion in moving station portion is the central rotation around disk.
Further, film-making portion also includes air extractor, and the air entry of air extractor is arranged on film cylinder connecting portion.
Further, film cylinder connecting portion is multiple, and station group includes the multiple specimen holder corresponding with multiple film cylinder connecting portions and multiple microscope slide seat.
Further, film-making portion also includes Tui Motong mechanism, and Tui Motong mechanism includes moving back film cylinder push rod and drive division, moves back film cylinder push rod and arranges along the outer surface of film cylinder connecting portion, and drive division drives and moves back the axially-movable along film cylinder connecting portion of the film cylinder push rod.
According to another aspect of the present invention, additionally provide a kind of film cylinder for liquid-based cell sample manufacturing, including: cylinder, it is internally formed cavity；Perforated membrane, is arranged on one end of cylinder, and perforated membrane is provided with multiple thief hole being communicated to cavity；Imbibition portion, the side of the close perforated membrane being arranged in cavity.
Further, imbibition portion covers the side towards cavity of perforated membrane.
Further, the diameter of thief hole is less than the diameter of liquid basal cell.
According to another aspect of the present invention, additionally provide a kind of liquid-based cell sample manufacturing method, including: one end with perforated membrane of film cylinder is immersed in the sample liquid of liquid basal cell, and make to be formed in the cavity of film cylinder negative pressure, extracting solution basal cell；By one end with perforated membrane of film cylinder by pressing to microscope slide, make the liquid basal cell that film cylinder extracts transfer on microscope slide, complete film-making；Wherein film cylinder includes cylinder and perforated membrane, and perforated membrane is arranged on one end of cylinder, forms cavity, perforated membrane is provided with multiple thief hole being communicated to cavity in film cylinder.
Further, by one end with perforated membrane of film cylinder by pressing to microscope slide, when making the liquid basal cell that film cylinder extracts transfer on microscope slide, liquid-based cell sample manufacturing method also includes: press down on to microscope slide by one end with perforated membrane of film cylinder, rises film cylinder after compression again.
Further, by one end with perforated membrane of film cylinder by pressing to microscope slide, when making the liquid basal cell that film cylinder extracts transfer on microscope slide, liquid-based cell sample manufacturing method also includes: in above-mentioned steps, and maintenance cavity is negative pressure state.
The liquid-based cell sample manufacturing equipment of the present invention has moving station portion, the multiple stations arranged thereon can be respectively moved to the operating position place corresponding in film-making portion by moving station portion, thus improving the automaticity of film-making flow process, decrease the ratio of manual operation film-making, namely simplify film-making flow process, improve film-making efficiency.
Accompanying drawing explanation
The Figure of description constituting the part of the application is used for providing a further understanding of the present invention, and the schematic description and description of the present invention is used for explaining the present invention, is not intended that inappropriate limitation of the present invention.In the accompanying drawings:
Fig. 1 illustrates the top view of the embodiment of the liquid-based cell sample manufacturing equipment according to the present invention；
Fig. 2 illustrates the liquid-based cell sample manufacturing equipment according to the present invention and is equipped with the front view of embodiment of film cylinder；
Fig. 3 illustrates the left view of the embodiment of the liquid-based cell sample manufacturing equipment according to the present invention；
Fig. 4 illustrates the schematic diagram of the film cylinder connecting portion of the liquid-based cell sample manufacturing equipment according to the present invention and the embodiment of the annexation of film cylinder；And
Fig. 5 illustrates the sectional view of the embodiment of the film cylinder according to the present invention.
Wherein, above-mentioned accompanying drawing includes the following drawings labelling:
10, body；20, lifting arm；30, film-making portion；31, film cylinder connecting portion；311, body；312, linkage section；313, valve；314, rubber ring；40, moving station portion；41, specimen holder；42, microscope slide seat；43, film cylinder base；44, pad；100, film cylinder；110, cylinder；111, big footpath section；112, path section；113, baffle plate；120, perforated membrane；130, imbibition portion.
Detailed description of the invention
It should be noted that when not conflicting, the embodiment in the application and the feature in embodiment can be mutually combined.Describe the present invention below with reference to the accompanying drawings and in conjunction with the embodiments in detail.
According to an aspect of the invention, it is provided a kind of liquid-based cell sample manufacturing equipment, as shown in Figures 1 to 4, this liquid-based cell sample manufacturing equipment includes: lifting arm 20；Film-making portion 30, is arranged on lifting arm 20, and film-making portion 30 includes the film cylinder connecting portion 31 arranged along the direction of motion of lifting arm 20；Moving station portion 40, including one or more station groups, each station group all includes the specimen holder 41 of the sample liquid for placing liquid basal cell and for placing the microscope slide seat 42 of microscope slide, moving station portion 40 can move and make specimen holder 41 and microscope slide seat 42 move to the operating position corresponding with film-making portion 30, when specimen holder 41 and microscope slide seat 42 are positioned at operating position, specimen holder 41 and microscope slide seat 42 are on the motion path of film cylinder connecting portion 31 or the extended line of motion path.
The liquid-based cell sample manufacturing equipment of the present invention has moving station portion 40, the multiple stations arranged thereon can be respectively moved in the operating position place of film-making portion 30 correspondence by moving station portion 40, thus improving the automaticity of film-making flow process, decrease the ratio of manual operation film-making, namely simplify film-making flow process, improve film-making efficiency.
The pelleter of membrane type suction filtration of the prior art, is broadly divided into following steps in film-making process: film-making portion drives filter membrane downsampled；Rotation turnback in film-making portion after sampling so that filter membrane upward, then carries out film-making；After film-making completes, film-making portion also needs to rotation turnback so that filter membrane is re-directed towards lower section, prepares film-making next time.Because film-making portion needs to commutate through multiple rotary in film-making process, waste portion of time, and make complicated in mechanical structure.
The liquid-based cell sample manufacturing equipment of the present invention in the course of the work, is arranged on after the film cylinder 100 on film cylinder connecting portion 31 samples in sample liquid, is directly pressed against on microscope slide by liquid basal cell, without rotating commutation, further simplify flow process, improve efficiency.
Additionally, the motion of the motion in moving station portion 40 and lifting arm 20 cooperates so that two parts each only carry out the motion on a direction, compared to the pelleter needing the commutation upset of film-making portion of the prior art, motion is also simpler.
Preferably, the bottom of microscope slide seat 42 is provided with pad 44, for instance foam-rubber cushion, to prevent microscope slide from crushing.
Preferably, station group also includes the film cylinder base 43 for placing film cylinder 100, moving station portion 40 can move and make film cylinder base 43 move to the operating position corresponding with film-making portion 30, and when film cylinder base 43 is positioned at operating position, film cylinder base 43 is on the motion path of film cylinder connecting portion 31.
In this embodiment, film cylinder 100 is placed in film cylinder base 43 in advance, and film cylinder connecting portion 31 can realize automatically filling film with the motion in film-making portion 30, eliminates the operation of staff frock film, further simplify operation, improves efficiency.
Preferably, in station group, specimen holder 41 is arranged between film cylinder base 43 and microscope slide seat 42.
When utilizing this liquid-based cell sample manufacturing equipment to carry out film-making, first film cylinder 100, sample liquid container, microscope slide are placed on the film cylinder base 43 in moving station portion 40, specimen holder 41 and microscope slide seat 42 accordingly；Starting device afterwards, lifting arm 20 is raised to a high position, and film cylinder base 43 is moved the operating position to film-making portion 30 by moving station portion 40, lifting arm 20 declines, the film cylinder connecting portion 31 in film-making portion 30 is connected with film cylinder 100, and lifting arm 20 rises, now equipped with film cylinder 100 on film cylinder connecting portion 31；Specimen holder 41 is moved the operating position to film-making portion 30 by moving station portion 40 afterwards, lifting arm 20 declines, the one end with perforated membrane 120 making film cylinder 100 is immersed in sample liquid, and extracting solution basal cell has extracted rear lifting arm 20 and risen with film-making portion 30 and film cylinder 100；Microscope slide seat 42 is moved the operating position to film-making portion 30 by moving station portion 40 afterwards, lifting arm 20 declines, the one end with perforated membrane 120 making film cylinder 100 is stamped on microscope slide, liquid basal cell is made to transfer on microscope slide, complete film-making, after film-making completes, lifting arm 20 rises with film-making portion 30 and film cylinder 100, manually can be taken off microscope slide.
Liquid-based cell sample manufacturing equipment also includes body 10, lifting arm 20 and moving station portion 40 and is arranged on body 10, and therefore body 10 is equivalent to base, and the dynamical element in moving station portion 40 is also disposed in body 10.
Preferably, as it is shown in figure 1, moving station portion 40 is in disk form, the direction of motion in moving station portion 40 is the central rotation around disk.Centrally disposed around disk of film cylinder base 43, specimen holder 41 and microscope slide seat 42.
And in this embodiment, moving station portion 40 has two station groups, each station group occupies the half of the circumference of disk, when so facilitating one station group of liquid-based cell sample manufacturing equipment utilization to carry out film-making, manually another station group can be operated, it is complete the microscope slide of film-making, film cylinder and sample liquid container including taking out, and new microscope slide, film cylinder and sample liquid container are in place, prepare for ensuing film-making.
Preferably, in the such as embodiment shown in Fig. 1 and 2, film cylinder connecting portion 31 is multiple, and station group includes the multiple specimen holder 41 corresponding with multiple film cylinder connecting portions 31 and multiple microscope slide seat 42.In this embodiment, film-making portion 30 has two film cylinder connecting portions 31, it is possible to two film cylinders of disposable installation 100 carry out film-making, and specimen holder 41 in each station group, microscope slide seat 42 and film cylinder base 43 are also two, corresponding with film cylinder connecting portion 31.
Preferably, film-making portion 30 also includes air extractor, and the air entry of air extractor is arranged on film cylinder connecting portion 31.
Air extractor can make air pump, in order to form negative pressure in the cavity of film cylinder 100, to be inhaled on perforated membrane 120 by the liquid basal cell in sample liquid.It is to be noted, the air extractor of the liquid-based cell sample manufacturing equipment of the present invention need not inflate formation malleation in film cylinder 100, namely, when the liquid-based cell sample manufacturing equipment utilizing the present invention carries out film-making, film cylinder 100 also can keep negative pressure state when compressing with microscope slide, or is pressure balance.
As shown in Figure 4, film cylinder connecting portion 31 includes body 311, linkage section 312, valve 313 and rubber ring 314, when film cylinder connecting portion 31 is installed in film-making portion 30, valve 313 is connected with the valve interface being positioned within film-making portion 30, so that the gas circuit in film cylinder connecting portion 31 is connected with air extractor, gas circuit runs through body 311 and linkage section 312, and the outlet of gas circuit is positioned at the end of linkage section 312.Linkage section 312 is for being connected with film cylinder 100, and the arranged outside of linkage section 312 has multiple rubber ring 314, and rubber ring 314, except playing the effect of elastic junctional membrane cylinder 100, also acts as the bubble-tight effect of offer.
Preferably, film-making portion 30 also includes Tui Motong mechanism, and Tui Motong mechanism includes moving back film cylinder push rod and drive division, moves back film cylinder push rod and arranges along the outer surface of film cylinder connecting portion 31, and drive division drives and moves back the axially-movable along film cylinder connecting portion 31 of the film cylinder push rod.The drive division of Tui Motong mechanism can for being independently arranged, it is also possible to adopts same power with lifting arm 20, and is controlled the motion of Tui Motong mechanism by electromagnetic switch.
In a preferred embodiment, after utilizing this liquid-based cell sample manufacturing equipment to complete film-making, film cylinder base 43 is moved the operating position to film-making portion 30 by moving station portion 40, the drive division driving of Tui Motong mechanism is moved back film cylinder push rod and is stretched out, the film cylinder 100 having made film-making separates with film cylinder connecting portion 31, falls in film cylinder base 43.Alternatively, film cylinder 100 falls into the sample liquid container being positioned in specimen holder 41, so conveniently processes refuse.
The liquid-based cell sample manufacturing equipment utilizing the present invention carries out liquid-based cell sample manufacturing, becomes tablet quality good, and film-making speed improves, it is possible to reach 240 per hour, far above existing pelleter.
According to another aspect of the present invention, additionally provide a kind of film cylinder for liquid-based cell sample manufacturing, as it is shown in figure 5, this film cylinder includes: cylinder 110, be internally formed cavity；Perforated membrane 120, is arranged on one end of cylinder 110, and perforated membrane 120 is provided with multiple thief hole being communicated to cavity；Imbibition portion 130, the side of the close perforated membrane 120 being arranged in cavity.
The film cylinder 100 of the present invention is provided with imbibition portion 130, and in sampling procedure, film cylinder 100 to immerse in sample liquid and imbibition, and imbibition portion 130 then absorbs unnecessary sample liquid, solves before film-making fluid seepage problem in sample transfer process, improves into tablet quality.
In addition, this film cylinder coordinates the liquid-based cell sample manufacturing equipment of the present invention to use, owing to keeping negative pressure in the cavity of film cylinder 100 in liquid-based cell sample manufacturing equipment film-making process, and can getting off to make not reflux through the liquid of perforated membrane 120 in constant negative pressure effect in imbibition portion 130, improves into tablet quality.
Preferably, imbibition portion 130 covers the side towards cavity of perforated membrane 120.
Imbibition portion 130 can make for sponge, it is also possible to makes for other materials with water absorbing capacity.
Preferably, the diameter of thief hole is less than the diameter of liquid basal cell.In film-making process, the liquid basal cell that sampling obtains not enters in the cavity of film cylinder 100 through perforated membrane 120, but it is stuck in the outside of the thief hole of perforated membrane 120, so when on film cylinder 100 coining to microscope slide, liquid basal cell is then transferred on microscope slide, and therefore the diameter of thief hole is less than the diameter of liquid basal cell.
As shown in Figure 5, the cylinder 110 of film cylinder 100 includes big footpath section 111 and path section 112, big footpath section 111 is for being connected with film cylinder connecting portion 31, and perforated membrane 120 and imbibition portion 130 are then positioned at path section 112, the outer rim end face that outer surface is film cylinder 100 of this wherein perforated membrane 120.
Preferably, cylinder 110 also includes baffle plate 113, and baffle plate 113 is arranged in the cavity of film cylinder 100, is used for carrying imbibition portion 130 and perforated membrane 120, baffle plate 113 is provided with the opening passed through for common gas.Film cylinder 100 on from path section 112 to the direction of big footpath section 111, includes perforated membrane 120, imbibition portion 130 and baffle plate 113 successively, and wherein imbibition portion 130 contacts setting with perforated membrane 120.
According to another aspect of the present invention, additionally provide a kind of liquid-based cell sample manufacturing method, including: one end with perforated membrane 120 of film cylinder 100 is immersed in the sample liquid of liquid basal cell, and make to be formed in the cavity of film cylinder 100 negative pressure, extracting solution basal cell；By one end with perforated membrane 120 of film cylinder 100 by pressing to microscope slide, make the liquid basal cell that film cylinder 100 extracts transfer on microscope slide, complete film-making；Wherein film cylinder 100 includes cylinder 110 and perforated membrane 120, and perforated membrane 120 is arranged on one end of cylinder 110, forms cavity, perforated membrane 120 is provided with multiple thief hole being communicated to cavity in film cylinder 100.
When the liquid-based cell sample manufacturing method utilizing the present invention carries out film-making, film cylinder 100 is press directly against microscope slide after sampling in sample liquid, on liquid basal cell coining to microscope slide, without rotating commutation, will simplify operation, improves efficiency.
Preferably, by one end with perforated membrane 120 of film cylinder 100 by pressing to microscope slide, when making the liquid basal cell that film cylinder 100 extracts transfer on microscope slide, liquid-based cell sample manufacturing method also includes: press down on to microscope slide by one end with perforated membrane 120 of film cylinder 100, rises film cylinder 100 after compression again.
Preferably, by one end with perforated membrane 120 of film cylinder 100 by pressing to microscope slide, when making the liquid basal cell that film cylinder 100 extracts transfer on microscope slide, liquid-based cell sample manufacturing method also includes: in above-mentioned steps, and maintenance cavity is negative pressure state.
Utilizing liquid-based cell sample manufacturing method provided by the invention, simple to operate, film-making speed is fast, and uniform quality is clear, superposition is few in flakes, without blank appearance.
The liquid-based cell sample manufacturing method of the present invention is that the liquid-based cell sample manufacturing equipment utilizing the present invention carries out.
The film cylinder used in the liquid-based cell sample manufacturing method of the present invention is film cylinder provided by the invention.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.