本申请是申请日为2014年4月23日，申请号为201410166957.3，发明名称为“自动制备细胞蜡块的装置及其方法”的分案申请。This application is a divisional application with an application date of April 23, 2014, an application number of 201410166957.3, and the title of the invention is "A device and method for automatically preparing cell wax blocks".
技术领域technical field
本发明涉及医疗电子器械领域，且特别涉及一种具有快速定位功能的细胞蜡块制备装置。The invention relates to the field of medical electronic equipment, and in particular to a cell wax block preparation device with a rapid positioning function.
背景技术Background technique
医学上处理体液细胞一般是把带有液体的细胞直接涂在玻璃片上，再通过显微镜进行观察。随着近年来细胞病理学的发展，以上这种常规技术制作的普通涂片的局限性越来越明显，如涂片存在唯一性，不能复制；不能永久保留；阻碍了新兴病理学技术如免疫组化、分子病理学技术的应用。Medical treatment of body fluid cells is generally to directly smear the cells with liquid on a glass slide, and then observe through a microscope. With the development of cytopathology in recent years, the limitations of ordinary smears produced by the above conventional techniques have become more and more obvious, such as smears are unique and cannot be copied; they cannot be permanently preserved; Application of histochemistry and molecular pathology techniques.
细胞蜡块则是把液体中的细胞提取后进行石蜡固定，固定后制成类似组织切片的形式。该方法解决了上述普通涂片存在的问题，还可避免因涂片损坏、丢失等可能引起的医患纠纷。同时制作细胞蜡块也是制备组织芯片的基础，该技术将广泛地应用在细胞病理学领域。The cell wax block is to extract the cells in the liquid, fix them in paraffin, and make them into a form similar to tissue slices after fixing. The method solves the problems existing in the above-mentioned common smear, and can also avoid doctor-patient disputes that may be caused by damage or loss of the smear. At the same time, making cell wax blocks is also the basis for preparing tissue chips, and this technology will be widely used in the field of cytopathology.
目前制作细胞蜡块基本上是采用手工方法来处理，处理的体液细胞可包括胸水、腹水、宫颈脱落细胞、浆膜腔积液、脑脊液、痰液等等。手工制作简要的步骤如下：将提取的体液倒入试管，将试管高速离心若干时间，去除试管上部包含了血液等干扰成分的上清液，使收集的液体细胞形成细胞团块；小心剥离下完整的细胞团块，用透水的滤纸包好，历经固定、脱水、透明、染色、浸蜡、冷却、包埋等一系列步骤，然后将包埋好的蜡块在切片机下切成约5μm的薄片，从而最终完成细胞蜡块。At present, the production of cell wax blocks is basically done manually. The processed body fluid cells may include pleural effusion, ascites, cervical exfoliated cells, serous cavity effusion, cerebrospinal fluid, sputum and so on. The brief steps of manual production are as follows: Pour the extracted body fluid into a test tube, centrifuge the test tube at high speed for a certain period of time, remove the supernatant containing blood and other interfering components in the upper part of the test tube, and make the collected liquid cells form cell clumps; carefully peel off the intact The cell aggregates were wrapped with permeable filter paper and went through a series of steps of fixation, dehydration, transparency, staining, wax dipping, cooling, and embedding. Thin slices, thus finalizing the cell wax block.
在以上步骤中，脱水、透明、浸蜡可以在组织处理机中自动完成，但其余步骤现在都是通过手工完成，耗时长约7～8个小时，期间某个步骤完成后，操作人员必须手工干预才能进行下一步，费时、费力。而且手工制作细胞蜡块，和操作人员的经验水平直接相关联，否则很可能出现蜡块质量参差不齐的情况；另外手工操作还可能会和福尔马林、二甲苯等试剂相接触，稍有不慎就会给操作人员的健康带来伤害。Among the above steps, dehydration, transparency, and wax dipping can be done automatically in the tissue processor, but the rest of the steps are now done manually, which takes about 7 to 8 hours. After a certain step is completed, the operator must do it manually. Intervention can proceed to the next step, which is time-consuming and laborious. Moreover, the hand-made cell wax block is directly related to the experience level of the operator, otherwise the quality of the wax block may be uneven; Carelessness will bring harm to the health of the operator.
发明内容Contents of the invention
本发明为了克服现有细胞蜡块制作需采用手工完成，不仅操作精度低且极易给操作者的身体带来伤害的问题，提供一种具有快速定位功能的细胞蜡块制备装置。In order to overcome the problem that the existing cell wax block production needs to be done manually, not only the operation accuracy is low, but also the operator is easily injured, the present invention provides a cell wax block preparation device with a rapid positioning function.
为了实现上述目的，本发明提供一种具有快速定位功能的细胞蜡块制备装置，包括转盘、试管、半透膜、取液装置、抽取装置和加压装置。转盘具有多个第一固定孔。试管的数量为至少一个，且固定于第一固定孔，试管包括第一管体和第二管体，第一管体和第二管体为可分离式连接，第二管体的底部具有开孔，第二管体呈中空的倒圆台状，且位于第二管体底部的开孔的面积小于第二管体底部的面积，第一管体的外侧壁设置有止挡块，止挡块的下边缘与第一管体和第二管体的连接面位于同一平面上。半透膜设置于第一管体和第二管体间，半透膜的上表面以及下表面均覆盖有金属网，且金属网以及半透膜均设置在第一管体和第二管体间。In order to achieve the above object, the present invention provides a cell wax block preparation device with a rapid positioning function, including a turntable, a test tube, a semi-permeable membrane, a liquid-taking device, an extraction device and a pressurizing device. The turntable has a plurality of first fixing holes. The number of the test tube is at least one, and it is fixed in the first fixing hole. The test tube includes a first tube body and a second tube body, the first tube body and the second tube body are detachably connected, and the bottom of the second tube body has an opening Hole, the second pipe body is hollow and rounded, and the area of the opening at the bottom of the second pipe body is smaller than the area of the bottom of the second pipe body, the outer wall of the first pipe body is provided with a stopper, the stopper The lower edge of the lower edge is on the same plane as the connecting surface of the first pipe body and the second pipe body. The semi-permeable membrane is arranged between the first pipe body and the second pipe body, the upper surface and the lower surface of the semi-permeable membrane are covered with metal mesh, and the metal mesh and the semi-permeable membrane are arranged between the first pipe body and the second pipe body between.
取液装置包括机械臂、吸管、第一导管以及工作泵。其中机械臂为试管添加细胞液体、试剂以及液体石蜡。机械臂包括底部、转动部以及夹持部，底部具有多个通孔，机械臂为中空结构，且转动部内设置有控制器。吸管设置于夹持部。第一导管经通孔以及机械臂内部与吸管相连接，用于液体的流通，第一导管上设置有电磁阀，一个第一导管对应设置有一个电磁阀，且电磁阀与控制器电性连接。工作泵设置于第一导管，将位于吸管下方的试剂瓶内的试剂抽取到吸管内，且一个第一导管对应设置有一个工作泵；其中工作泵与控制器电性连接。The liquid taking device includes a mechanical arm, a suction pipe, a first conduit and a working pump. The robotic arm adds cell liquid, reagents and liquid paraffin to the test tube. The mechanical arm includes a bottom, a rotating part and a clamping part, the bottom has a plurality of through holes, the mechanical arm is a hollow structure, and a controller is arranged in the rotating part. The straw is arranged on the holding part. The first conduit is connected to the suction pipe through the through hole and the inside of the mechanical arm, and is used for the circulation of the liquid. The first conduit is provided with a solenoid valve, and one first conduit is correspondingly provided with a solenoid valve, and the solenoid valve is electrically connected to the controller. . The working pump is arranged in the first conduit to draw the reagent in the reagent bottle under the suction pipe into the suction pipe, and one first conduit is correspondingly provided with a working pump; wherein the working pump is electrically connected with the controller.
抽取装置包括固定件和第二导管，固定件为中空结构，固定件的顶部开口与第二管体底部的开孔相固定连接，固定件的底部开口与第二导管的一端相固定连接，固定件的上部设置有与中空倒圆台状的第二管体相对应的凹槽，且凹槽的两边设置有工作臂,凹槽的侧壁设置有缓冲件。加压装置与第二导管的另一端相连接，通过第二导管为试管底部提供正压或负压。The extracting device includes a fixing piece and a second conduit, the fixing piece is a hollow structure, the top opening of the fixing piece is fixedly connected with the opening at the bottom of the second pipe body, the bottom opening of the fixing piece is fixedly connected with one end of the second conduit, and the fixed piece The upper part of the part is provided with a groove corresponding to the second tubular body in the shape of a hollow rounded table, and the two sides of the groove are provided with working arms, and the side walls of the groove are provided with buffer pieces. The pressurizing device is connected with the other end of the second conduit to provide positive or negative pressure to the bottom of the test tube through the second conduit.
综上所述，本发明提供的具有快速定位功能的细胞蜡块制备装置，取液装置内的第一导管以及工作泵将待制备的细胞液体以及位于试剂瓶内的试剂通过机械臂以及吸管添加到试管中。机械取液代替了传统的人工取液，试剂不与操作者接触，操作者身体不会受到伤害。通过控制工作泵也可达到控制试剂的添加量，与手动操作相比精度更高。通过设置加压装置在浸泡福尔马林、脱水、染色、透明以及浸泡石蜡等步骤中不断重复给试管底部增加正压或负压，使得位于半透膜上的细胞充分的与所添加的试剂反应。用户只需按需要设置好加压装置加正压和负压的时间，即可自动完成上述过程。In summary, the cell wax block preparation device with rapid positioning function provided by the present invention, the first conduit and the working pump in the liquid taking device add the cell liquid to be prepared and the reagent in the reagent bottle through the mechanical arm and the suction pipe into the test tube. The mechanical liquid extraction replaces the traditional manual liquid extraction, the reagent does not come into contact with the operator, and the operator will not be harmed. The amount of reagent added can also be controlled by controlling the working pump, which has higher precision than manual operation. By setting the pressurizing device, the positive or negative pressure is repeatedly added to the bottom of the test tube during the steps of immersion in formalin, dehydration, staining, clearing and immersion in paraffin, so that the cells on the semi-permeable membrane can fully communicate with the added reagents. reaction. The user only needs to set the positive pressure and negative pressure time of the pressurization device according to the needs, and the above process can be completed automatically.
此外，在半透膜的上表面以及下表面均设置金属网，当加热装置对石蜡管路以及试管时，金属网具有良好的导热功能可将试管上的热量传递到半透膜上，使得半透膜上的石蜡在浸泡石蜡过程中保持液态。In addition, a metal mesh is set on the upper surface and the lower surface of the semipermeable membrane. When the heating device is on the paraffin pipeline and the test tube, the metal mesh has a good heat conduction function and can transfer the heat on the test tube to the semipermeable membrane, making the semipermeable membrane The paraffin on the permeable membrane remains liquid during paraffin immersion.
为让本发明的上述和其它目的、特征和优点能更明显易懂，下文特举较佳实施例，并配合附图，作详细说明如下。In order to make the above and other objects, features and advantages of the present invention more comprehensible, preferred embodiments are described below in detail with accompanying drawings.
附图说明Description of drawings
图1所示为本发明一实施例提供的具有快速定位功能的细胞蜡块制备装置示意图；Figure 1 is a schematic diagram of a cell block preparation device with rapid positioning function provided by an embodiment of the present invention;
图2所示为图1中试管、固定件以及加压装置的截面示意图；Fig. 2 is a schematic cross-sectional view of the test tube, the fixture and the pressurizing device in Fig. 1;
图3所示为图1所示的具有快速定位功能的细胞蜡块制备装置的工作流程图。Fig. 3 is a working flowchart of the cell wax block preparation device with rapid positioning function shown in Fig. 1 .
具体实施方式Detailed ways
图1所示为本发明一实施例提供的具有快速定位功能的细胞蜡块制备装置示意图；图2所示为图1中试管、固定件以及加压装置的截面示意图；图3所示为图1所示的具有快速定位功能的细胞蜡块制备装置的工作流程图。请一并参阅图1至图3。Fig. 1 is a schematic diagram of a cell wax block preparation device with a rapid positioning function provided by an embodiment of the present invention; Fig. 2 is a schematic cross-sectional view of a test tube, a fixture and a pressurizing device in Fig. 1; Fig. 3 is a diagram 1 shows the working flow diagram of the cell wax block preparation device with rapid positioning function. Please refer to Figure 1 to Figure 3 together.
本发明提供一种具有快速定位功能的细胞蜡块制备装置，包括转盘1、试管2、半透膜3、取液装置4、抽取装置5以及加压装置6。转盘1具有多个第一固定孔11。试管2的数量为至少一个，且固定于第一固定孔11。试管2包括第一管体21和第二管体22，第一管体21和第二管体22为可分离式连接，第二管体22的底部具有开孔221。半透膜3设置于第一管体21和第二管体22间。取液装置4包括机械臂41、吸管42、第一导管43以及工作泵44。其中机械臂41为试管2添加试剂或液体石蜡。机械臂41包括底部411、转动部412以及夹持部413，底部411具有多个通孔。机械臂41为中空结构，且转动部412内设置有控制器。吸管42设置于夹持部413。第一导管43经通孔以及机械臂41内部与吸管42相连接，用于液体的流通。工作泵44设置于第一导管43，将位于吸管42下方的试剂瓶内的试剂抽取到吸管42内，且一个第一导管43对应设置有一个工作泵44。抽取装置5包括固定件51和第二导管52，固定件51为中空结构，固定件51的顶部开口与第二管体22底部的开孔221相固定连接，固定件51的底部开口与第二导管52的一端相固定连接。加压装置6与第二导管52的另一端相连接，通过第二导管52为试管2底部提供正压或负压。其中，第一导管43上还设置有电磁阀45，且一个第一导管43对应设置有一个电磁阀45。The present invention provides a cell wax block preparation device with rapid positioning function, which includes a turntable 1 , a test tube 2 , a semipermeable membrane 3 , a liquid taking device 4 , an extracting device 5 and a pressurizing device 6 . The turntable 1 has a plurality of first fixing holes 11 . There is at least one test tube 2 and it is fixed in the first fixing hole 11 . The test tube 2 includes a first tube body 21 and a second tube body 22 , the first tube body 21 and the second tube body 22 are detachably connected, and the bottom of the second tube body 22 has an opening 221 . The semipermeable membrane 3 is disposed between the first tube body 21 and the second tube body 22 . The liquid taking device 4 includes a mechanical arm 41 , a suction tube 42 , a first conduit 43 and a working pump 44 . Wherein the mechanical arm 41 adds reagent or liquid paraffin to the test tube 2 . The mechanical arm 41 includes a bottom 411 , a rotating portion 412 and a clamping portion 413 , and the bottom 411 has a plurality of through holes. The mechanical arm 41 is a hollow structure, and a controller is disposed in the rotating part 412 . The suction tube 42 is disposed on the clamping portion 413 . The first conduit 43 is connected to the suction pipe 42 through the through hole and the inside of the mechanical arm 41 for the circulation of liquid. The working pump 44 is arranged on the first conduit 43 to draw the reagent in the reagent bottle below the suction pipe 42 into the suction pipe 42 , and one first conduit 43 is correspondingly provided with one working pump 44 . The extracting device 5 includes a fixing piece 51 and a second conduit 52, the fixing piece 51 is a hollow structure, the top opening of the fixing piece 51 is fixedly connected with the opening 221 at the bottom of the second pipe body 22, the bottom opening of the fixing piece 51 is connected with the second One end of the conduit 52 is fixedly connected. The pressurizing device 6 is connected with the other end of the second conduit 52 to provide positive or negative pressure to the bottom of the test tube 2 through the second conduit 52 . Wherein, a solenoid valve 45 is also provided on the first conduit 43 , and one solenoid valve 45 is correspondingly provided on one first conduit 43 .
于本实施例中，取液装置4有两套，其中一套用于抽取待制作的细胞液体和福尔马林、酒精以及二甲苯等液体，且该套取液装置中机械臂41的底部411具有四个通孔，分别有四条第一导管43穿过该相应的通孔与该取液装置4上的吸管42相连接，分别用于吸取福尔马林、浓度为95％酒精、无水酒精以及二甲苯，另一套取液装置4用于抽取石蜡。然而，本发明对此不作任何限定。于其它实施例中，可采用一套取液装置4，但在机械臂41的底部设置五个以上的通孔，并设置两个夹持部413以及两个吸管42，其中一个吸管42用于吸取福尔马林等试剂以及待制备的细胞液体，而另一吸管42用于吸取石蜡。In this embodiment, there are two sets of liquid-taking devices 4, one of which is used to extract liquids such as cell liquid to be prepared and formalin, alcohol, and xylene, and the bottom 411 of the mechanical arm 41 in the set of liquid-taking devices There are four through holes, and four first conduits 43 are connected to the suction pipe 42 on the liquid taking device 4 through the corresponding through holes respectively, and are respectively used to suck formalin, concentration of 95% alcohol, anhydrous Alcohol and xylene, another set of liquid-taking device 4 is used for extracting paraffin. However, the present invention does not make any limitation thereto. In other embodiments, a set of liquid extraction device 4 can be used, but more than five through holes are provided at the bottom of the mechanical arm 41, and two clamping parts 413 and two suction pipes 42 are provided, one of which is used for Aspirate reagents such as formalin and the cell liquid to be prepared, while another suction pipe 42 is used for aspirating paraffin.
下面结合图1和图3对本发明提供的具有快速定位功能的细胞蜡块制备装置的工作流程进行详细说明。The working process of the cell wax block preparation device with rapid positioning function provided by the present invention will be described in detail below with reference to FIG. 1 and FIG. 3 .
该过程起始于取样，将希望制取细胞蜡块的细胞液体吸取入具有半透膜3的试管2内(步骤S101)。吸取完后对吸管进行冲洗。The process starts with sampling, and the cell fluid for which the cell wax block is to be prepared is drawn into the test tube 2 with the semi-permeable membrane 3 (step S101). Rinse the straw after drinking.
浸泡福尔马林(步骤S102)。用户打开与盛放福尔马林的试剂瓶相连接的第一导管43上的工作泵44以及电磁阀45，吸管42吸取5ml浓度为10％的中性福尔马林至已放置有细胞液体的试管2内。Soak formalin (step S102). The user opens the working pump 44 and the solenoid valve 45 on the first conduit 43 connected to the reagent bottle filled with formalin, and the suction pipe 42 sucks 5ml of concentration of 10% neutral formalin until the cell liquid has been placed. in test tube 2.
开启加压装置6，通过第二导管52在试管底部增加负压，使得水分、小分子以及福尔马林液体通过半透膜3，而细胞液体中的细胞则被滞留在半透膜3上。当半透膜3上积累的细胞较多堵住半透膜3时。设置加压装置6，反转控制泵，在试管底部增加正压，将堵在半透膜3上的细胞重新压回细胞液体中。之后再重新增加负压，将混合试剂的液体通过半透膜3吸入废液瓶中。不断重复上述过程，使得半透膜3上的所有细胞均充分浸泡福尔马林，该过程历时2小时，在该过程必须保证试管内至少有2ml以上的福尔马林液体。Turn on the pressurizing device 6, increase the negative pressure at the bottom of the test tube through the second conduit 52, so that water, small molecules and formalin liquid pass through the semi-permeable membrane 3, while the cells in the cell liquid are retained on the semi-permeable membrane 3 . When the cells accumulated on the semi-permeable membrane 3 block up the semi-permeable membrane 3 more. The pressurizing device 6 is set, the reverse control pump is used, and the positive pressure is increased at the bottom of the test tube to press the cells blocked on the semi-permeable membrane 3 back into the cell liquid. Afterwards, the negative pressure is increased again, and the liquid of the mixed reagent is sucked into the waste liquid bottle through the semipermeable membrane 3 . The above process is repeated continuously, so that all the cells on the semipermeable membrane 3 are fully soaked in formalin. This process lasts for 2 hours. During this process, it must be ensured that there is at least 2ml of formalin liquid in the test tube.
为了保证试管内至少有2ml以上的福尔马林液体，本实施例中，通过在试管2的侧壁上设置传感器，该传感器与控制器电性连接。控制器实时检测试管2内的液面高度，当试管2内的液面高度低于2ml时，控制器输出控制信号开启工作泵44以及电磁阀45，吸管42吸取福尔马林至试管内，实现自动浸泡福尔马林的过程。In order to ensure that there is at least 2ml of formalin liquid in the test tube, in this embodiment, a sensor is provided on the side wall of the test tube 2, and the sensor is electrically connected to the controller. The controller detects the liquid level in the test tube 2 in real time. When the liquid level in the test tube 2 is lower than 2ml, the controller outputs a control signal to turn on the working pump 44 and the electromagnetic valve 45, and the suction pipe 42 draws formalin into the test tube. Realize the process of soaking formalin automatically.
脱水(步骤S103)，该脱水过程包括一次脱水和二次脱水，一次脱水和二次脱水的过程与浸泡福尔马林的过程相同，只是一次脱水中所用的试剂是浓度为95％的酒精，脱水时间是1.5小时。而二次脱水所用的试剂是无水酒精，而脱水时间也是1.5小时。Dehydration (step S103), this dehydration process comprises primary dehydration and secondary dehydration, the process of primary dehydration and secondary dehydration is the same as the process of immersing formalin, only the reagent used in primary dehydration is the alcohol that concentration is 95%, The dehydration time is 1.5 hours. The reagent used for secondary dehydration is absolute alcohol, and the dehydration time is also 1.5 hours.
染色(步骤S104)，在试管2内添加0.8ml伊红对已二次脱水的细胞进行染色，不断重复对试管底部增加负压、正压以及负压，使得半透膜3上滞留的细胞充分染色，该过程历时10分钟。Staining (step S104), adding 0.8ml eosin to the test tube 2 to stain the secondary dehydrated cells, repeatedly increasing negative pressure, positive pressure and negative pressure at the bottom of the test tube, so that the cells retained on the semipermeable membrane 3 are fully Dyeing, the process lasted 10 minutes.
透明(步骤S105)，在试管内添加二甲苯对已染色后的细胞进行透明处理，不断重复对试管2底部增加负压、正压以及负压，该过程历时30分钟。Transparent (step S105), add xylene in the test tube to perform transparent treatment on the stained cells, and repeatedly increase the negative pressure, positive pressure and negative pressure on the bottom of the test tube 2, and the process lasts for 30 minutes.
浸泡石蜡(步骤S106)，加热盛有石蜡的试剂瓶、石蜡吸取管路以及试管，将试剂瓶内的石蜡融化，将融化后的石蜡加入至试管2内，对试管2底部不断重复增加负压、正压以及负压，该过程历时1.5小时，将透明处理后细胞上残留的二甲苯置换出来，在该过程中试管2的温度在60℃～70℃。本实施例中，为防止在步骤S106中半透膜3上的石蜡冷却凝固，在半透膜3的上表面以及下表面均覆盖有金属网7，且金属网7以及半透膜3均设置在第一管体和第二管体间，金属网7可快速的将试管2的温度传递给半透膜3，使得半透膜3的温度维持在60℃～70℃。此外，金属网7还可对半透膜2起到支撑作用。Soak the paraffin (step S106), heat the reagent bottle containing the paraffin, the paraffin suction line and the test tube, melt the paraffin in the reagent bottle, add the melted paraffin into the test tube 2, and repeatedly increase the negative pressure on the bottom of the test tube 2 , positive pressure and negative pressure. This process lasted for 1.5 hours to replace the residual xylene on the cells after the transparent treatment. During this process, the temperature of the test tube 2 was 60°C to 70°C. In this embodiment, in order to prevent the paraffin on the semipermeable membrane 3 from cooling and solidifying in step S106, the upper surface and the lower surface of the semipermeable membrane 3 are covered with metal mesh 7, and the metal mesh 7 and the semipermeable membrane 3 are all set Between the first tube body and the second tube body, the metal mesh 7 can quickly transfer the temperature of the test tube 2 to the semi-permeable membrane 3, so that the temperature of the semi-permeable membrane 3 is maintained at 60°C-70°C. In addition, the metal mesh 7 can also support the semi-permeable membrane 2 .
在上述步骤S102～步骤S106中，通过加压装置6来使得滞留在半透膜3上的细胞与每一步添加的试剂或石蜡重复反应，所有的过程均是自动完成，用户只需设置每一步骤的时间以及加压装置6加正压或负压的时间即可。操作者无需接触任何的试剂，不会危害操作者的身体健康。进一步的，通过增加电磁阀45，通过控制电磁阀45的阀门开度，可实现试剂的精确吸取。而增加传感器来检测试管2内的液面高度，并将检测到的信号传递给控制器，控制器根据该检测信号控制电磁阀45以及工作泵44的工作状态，实现自动控制。In the above steps S102 to S106, the pressurizing device 6 is used to make the cells retained on the semi-permeable membrane 3 repeatedly react with the reagent or paraffin added in each step. All the processes are automatically completed, and the user only needs to set each The time of the step and the time for the pressurizing device 6 to apply positive pressure or negative pressure are enough. The operator does not need to touch any reagents, and will not endanger the health of the operator. Furthermore, by adding the solenoid valve 45 and controlling the valve opening of the solenoid valve 45, precise suction of the reagent can be realized. A sensor is added to detect the liquid level in the test tube 2, and the detected signal is transmitted to the controller, and the controller controls the operating states of the electromagnetic valve 45 and the working pump 44 according to the detection signal to realize automatic control.
当步骤S106完成且试管2冷却后将半透膜3上的细胞蜡块去除放入包埋框内进行包埋并将包埋好的细胞蜡块置于切片机下切片(步骤S107)。When step S106 is completed and the test tube 2 is cooled, the cell wax block on the semipermeable membrane 3 is removed and placed in an embedding frame for embedding, and the embedded cell wax block is placed under a microtome for sectioning (step S107).
于本实施例中，第二管体22呈中空的倒圆台状，且位于第二管体22底部的开孔221的面积小于第二管体22底部的面积。与该倒圆台状结构相对应的固定件51的上部设置有凹槽511，且凹槽511的两边设置有工作臂512。倒圆台状结构的第二管体22可将液体集中流入中空的固定件51内。同时，第二管体22底部的开孔221的面积小于第二管体22底部的面积，液体不会发生任何渗漏。In this embodiment, the second tube body 22 is hollow and rounded, and the area of the opening 221 at the bottom of the second tube body 22 is smaller than the area of the bottom of the second tube body 22 . A groove 511 is disposed on the upper portion of the fixing member 51 corresponding to the rounded truncated structure, and working arms 512 are disposed on both sides of the groove 511 . The second tube body 22 of rounded frustum shape can concentrate the liquid into the hollow fixing member 51 . At the same time, the area of the opening 221 at the bottom of the second tube body 22 is smaller than the area of the bottom of the second tube body 22, so the liquid will not leak.
倒圆台状结构可更好的保护第二管体22，防止第二管体22发生碎裂。为进一步的保护第二管体22，不仅在凹槽511的侧壁设置有缓冲件513，同时还在第一管体21的外侧壁设置有止挡块211，止挡块211的下边缘与第一管体21和第二管体22的连接面位于同一平面上。当固定件51上升与第二管体22固定连接时，止挡块211对工作臂512进行阻挡，防止固定件51过分上升，从而使得第二管体22的底部受挤压而发生碎裂。The rounded truncated structure can better protect the second tube body 22 and prevent the second tube body 22 from being broken. In order to further protect the second pipe body 22, not only a buffer member 513 is provided on the side wall of the groove 511, but also a stop block 211 is provided on the outer wall of the first pipe body 21, and the lower edge of the stop block 211 is in contact with the outer wall of the first pipe body 21. The connection surfaces of the first pipe body 21 and the second pipe body 22 are located on the same plane. When the fixing part 51 rises and is fixedly connected with the second pipe body 22 , the stop block 211 blocks the working arm 512 to prevent the fixing part 51 from rising too much, so that the bottom of the second pipe body 22 is crushed and broken.
综上所述，本发明提供的具有快速定位功能的细胞蜡块制备装置及其方法，取液装置4内的第一导管43以及工作泵44将待制备的细胞液体以及位于试剂瓶内的试剂通过机械臂41以及吸管42添加到试管2中。机械取液代替了传统的人工取液，试剂不与操作者接触，操作者身体不会受到伤害。通过控制工作泵44也可达到控制试剂的添加量，与手动操作相比精度更高。通过设置加压装置6在浸泡福尔马林、脱水、染色、透明以及浸泡石蜡等步骤中不断重复给试管2底部增加正压或负压，使得位于半透膜3上的细胞充分的与所添加的试剂反应。用户只需按需要设置好加压装置6加正压和负压的时间，即可自动完成上述过程。To sum up, in the cell wax block preparation device and method thereof with rapid positioning function provided by the present invention, the first conduit 43 and the working pump 44 in the liquid taking device 4 transfer the cell liquid to be prepared and the reagents in the reagent bottle Add to the test tube 2 through the mechanical arm 41 and the suction pipe 42. The mechanical liquid extraction replaces the traditional manual liquid extraction, the reagent does not come into contact with the operator, and the operator will not be harmed. The addition amount of the reagent can also be controlled by controlling the working pump 44, which has higher precision than manual operation. By arranging the pressurizing device 6, during the steps of soaking in formalin, dehydration, staining, transparent and soaking in paraffin, etc., the positive or negative pressure is repeatedly added to the bottom of the test tube 2, so that the cells located on the semipermeable membrane 3 are fully compatible with the Added reagents react. The user only needs to set the time for the pressurizing device 6 to apply positive pressure and negative pressure as required, and the above process can be automatically completed.
此外，在半透膜3的上表面以及下表面设置金属网7，当加热装置对石蜡管路以及试管时，金属网7具有良好的导热功能可将试管上的热量传递到半透膜3上，使得半透膜3上的石蜡在浸泡石蜡过程中保持液态。In addition, a metal mesh 7 is provided on the upper surface and the lower surface of the semipermeable membrane 3. When the heating device faces the paraffin pipeline and the test tube, the metal mesh 7 has a good heat conduction function and can transfer the heat on the test tube to the semipermeable membrane 3. , so that the paraffin on the semi-permeable membrane 3 remains liquid during the process of soaking in the paraffin.
虽然本发明已由较佳实施例揭露如上，然而并非用以限定本发明，任何熟知此技艺者，在不脱离本发明的精神和范围内，可作些许的更动与润饰，因此本发明的保护范围当视权利要求书所要求保护的范围为准。Although the present invention has been disclosed above by preferred embodiments, it is not intended to limit the present invention. Any skilled person can make some changes and modifications without departing from the spirit and scope of the present invention. Therefore, the present invention The scope of protection should be subject to the scope of protection required by the claims.
| Application Number | Priority Date | Filing Date | Title |
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| CN201610247500.4ACN105675373B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with fast positioning function |
| Application Number | Priority Date | Filing Date | Title |
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| CN201410166957.3ACN103969096B (en) | 2014-04-23 | 2014-04-23 | Device and method for automatically preparing cell wax blocks |
| CN201610247500.4ACN105675373B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with fast positioning function |
| Application Number | Title | Priority Date | Filing Date |
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| CN201410166957.3ADivisionCN103969096B (en) | 2014-04-23 | 2014-04-23 | Device and method for automatically preparing cell wax blocks |
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| CN105675373A CN105675373A (en) | 2016-06-15 |
| CN105675373Btrue CN105675373B (en) | 2018-11-09 |
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| CN201610247500.4AExpired - Fee RelatedCN105675373B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with fast positioning function |
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