Working electrode bioreacter and electric pole type test-stripsTechnical field
The present invention relates to technical field of electrochemical detection, particularly working electrode bioreacter and electric pole type test-strips.
Background technology
POCT, real-time test (point-of-caretesting), refers to the clinical detection of carrying out on patient side, and not necessarily clinical examination teacher carries out usually.In most cases POCT analyzes at once in sampling location, saves the complex process program of sample when laboratory inspection, obtains a class new method of assay fast.Present POCT has become the important component part of laboratory medicine, because its experiment instrument miniaturization, simple operation, just-in-time of reporting the result are subject to the favor of people.
Electrochemical test bar based on enzyme amperometric biosensor principle extensively should in various instant detection (POCT) product, as commercial blood sugar, blood ketone, blood fat and serum creatinine electrochemical test bar.Generally, the enzyme-specific molecule and coenzyme or electron mediator that identify target analytes are contained in electrochemical test bar surface, enzymatic oxidation reduction reaction is there is in target analytes on electrochemical test bar surface, be converted to the electric signal (oxidation or reduction current) that can collect and measure, the quantitative detection to target analytes can be realized by enzyme biochemistry reaction (signal conversion) and electrochemical reaction (signal collection and measurement) two steps.
Usually, target analytes produces in the process of enzymatic reaction current signal at working electrode surface, the reductibility chaff interference of the multiple endogenous or external source existed in blood also can participate in the generating process of current signal, measurement result is caused to the interference of plus or minus, wherein common with the interference of ascorbic acid.For the electrochemical test bar (as blood sugar or blood ketone electrochemical test bar) taking oxidation current as detection signal, ascorbic acid can be oxidized by Electrocatalytic Oxidation or oxidized type electron mediator at electrode surface, therefore the interference current of the real current that institute's oxygen determination galvanic current is target molecule to be produced and ascorbic acid generation is cumulative, causes measurement result to be overgauge.For the electrochemical test bar (as blood fat or serum creatinine electrochemical test bar) taking reduction current as detection signal, ascorbic acid can with hydrogen peroxide generation enzyme (peroxidase) enzymatic oxidation reduction reaction, reduce the content of hydrogen peroxide with reductibility electron mediator generation enzymatic oxidation reduction reaction, cause the true reduction current recorded to reduce, measurement result produces minus deviation.Usually, reduce or eliminate ascorbic acid to the interference of electrochemical test strip mainly based on following several approach:
Approach one: the interference current signal of deduction working electrode surface indirectly.Adopt multi-electrode pattern (sensor of such as three-electrode structure), ascorbic acid produces direct oxidation current signal at working electrode and background electrode surface simultaneously, again the current signal of background electrode is deducted from working electrode, reach the object reducing interference.This method effectively can reduce the interference of ascorbic acid, but electrochemical test strip used need adopt multi-electrode mode of operation, and electrode structure is complicated, not easily manufactures.In addition, the method, at present only for improving with oxidation current the antijamming capability of the electrochemical test bar Ascorbic Acid being signal, not being suitable for and reducing ascorbic acid to the negative interference of electrochemical test strip taking reduction current as detection signal.
Approach two: examination bar structure comprises anti-interference layer, as patent CN201110294269.1, the scheme mentioned in CN201210407884.3 and CN201510188547.3, routine examination bar structure increases an anti-interference layer, anti-interference layer is kept apart with the responding layer of examination bar itself, when making sample by anti-interference layer, interfering material (as ascorbic acid) reacts with the anti-interference enzyme (ascorbic acid oxidase as traditional) in anti-interference layer, interfering material in sample is eliminated, then sample enter into again examination bar responding layer react, generate the corresponding electric signal of thing to be detected.This scheme makes the structure of examination bar become complicated, and at least must increase to some extent on the thickness of examination bar, similarly manufacturing cost is higher, and in industrial processes, be difficult to batch production, and examination bar poor quality controls.
Approach three: the interference current signal directly reducing working electrode surface.The ascorbic acid of direct minimizing or clearing electrode near surface, reduces or eliminates ascorbic acid producing interference current signal at electrode surface.Usually, ascorbic acid (as blood) in physiological environment is electronegative, the electronegative macromolecule membrane of one deck (as sulfonate fluoropolymer Nafion) can be covered at working electrode surface, utilize Coulomb repulsion principle electronegative ascorbic acid and working electrode to be separated, reduce the amount of electrode surface ascorbic acid.This scheme is feasible in theory, but in actual production, because this macromolecule membrane cost is higher, the isolation effect of Ascorbic Acid is difficult to control simultaneously, and very difficult guarantee can produce a desired effect at every turn, does not possess practicality with the technical merit of present stage.
Approach four: formation mixed enzyme that anti-interference enzyme and thing enzyme to be detected are put together, as the scheme mentioned in patent CN200710119127.5, traditional ascorbic acid oxidase is adopted to participate in reaction, although jamproof effect can be reached in theory, also can adopt the examination bar of existing this simple structure simultaneously, use also comparatively simple.But, after experimental result shows that traditional ascorbic acid oxidase adds thing enzyme to be detected, fail obviously to improve the antijamming capability trying bar Ascorbic Acid, add the chemical reaction that this traditional ascorbic acid oxidase can produce some the unknowns simultaneously, severe jamming is produced (as reduced examination bar precision to the enzyme reaction current responsing signal of electrochemical test strip self, reduce current-responsive sensitivity, reduce the range of linearity).Therefore, in the disposable electrochemical test strip of droppable commercialization, rarely have employing directly to mix the method for thing enzyme to be detected and traditional ascorbic acid oxidase to reduce the interference of ascorbic acid at present.
Therefore, develop a kind of effective reduction or eliminate the method disturb electrochemical test bar of ascorbic acid, the accuracy of raising electrochemical test bar is significant.
Summary of the invention
In view of this, the invention provides working electrode bioreacter and electric pole type test-strips.This invention adopts direct compound target to analyze the method for the single-minded enzyme of thing (biology enzyme corresponding with thing to be detected) and novel ascorbic acid oxidase (N-ASO) to reduce the interference of ascorbic acid in electric pole type test-strips.Anti-interference method of the present invention is applicable to the electrochemical test bar adopting single or multiple working electrode system, not only can reduce ascorbic acid to the interference of electrochemical test bar taking oxidation current as detection signal, also can reduce ascorbic acid to the interference of electrochemical test bar taking reduction current as detection signal.
In order to realize foregoing invention object, the invention provides following technical scheme:
The invention provides a kind of working electrode bioreacter, comprise microbe-derived ascorbic acid oxidase, the biology enzyme corresponding with thing to be detected, and electron mediator.
Compared with traditional ascorbic acid oxidase (ASO), in the present invention, novel ascorbic acid oxidase (representing with N-ASO) is in the source of enzyme, and physicochemical property and aspect, enzymic catalytic reaction path are distinguished to some extent, as shown in table 1.This N-ASO is a kind of novel ascorbic acid oxidase of the wild Company in Japanese sky, its enzyme source is microbial body, and the source of traditional ASO is cucumber (plant extracts), both physicochemical properties such as molecular weight, isoelectric point there is difference, therefore both there are differences on molecular structure and composition, this structure is different with the enzymic catalytic reaction path that the difference on composition result in both Ascorbic Acids, the oxidable ascorbic acid of N-ASO produces hydrogen peroxide, and the product of traditional ASO is water.Simultaneously, the present invention studies and finds this N-ASO directly to mix with the single-minded enzyme of target analytes the interference that rear made electrochemical test strip not only can effectively reduce ascorbic acid, and any interference (if do not affected the precision of examination bar, not reducing current-responsive sensitivity) is not produced on the enzyme reaction current responsing signal of the single-minded enzyme of target analytes.This N-ASO can for reducing ascorbic acid to the interference of electrochemical test bar taking oxidation current as detection signal, can be used for again reducing ascorbic acid to the interference of electrochemical test bar taking reduction current as detection signal:
One, ascorbic acid is reduced to the interference of electrochemical test bar taking oxidation current as detection signal.
In the electrochemical test bar taking oxidation current as detection signal, its oxidation current is originating primarily from the electrochemical oxidation reactions of reduced form electron mediator under positive potential that reaction generates.For this type of electrochemical test strip adopting single working electrode, add novel ascorbic acid oxidase at working electrode surface and Ascorbic Acid Oxidation can be generated hydrogen peroxide, and hydrogen peroxide produces at electrode surface generation electro-oxidation reaction the degree just disturbed much smaller than ascorbic acid, therefore can will reduce or eliminate the interference of ascorbic acid to this type of electrochemical test strip.
In addition, be the antijamming capability that a step improves examination bar, the electrochemical test strip of dual-electrode can be adopted.First working electrode is target analytes electrode, survey electric current and be designated as I1, the second working electrode is blank electrode, survey electric current and be designated as I2, all add novel ascorbic acid oxidase at the first and second working electrode surfaces.In actual measurement, the all oxidized generation hydrogen peroxide of ascorbic acid of the first and second working electrode surfaces, under positive potential, all there is electrooxidation to a certain degree in the hydrogen peroxide of the first and second electrode surfaces, generating portion just disturbs oxidation current, by the first working electrode currents I1deduct the second working electrode currents I2obtain the electric current I (I=I after background correction current signal1-I2), this part can be eliminated and just disturb oxidation current, improve the antijamming capability of electrochemical test strip Ascorbic Acid further.
Two, ascorbic acid is reduced to the interference of electrochemical test bar taking reduction current as detection signal.
In the electrochemical test bar taking reduction current as detection signal, its reduction current is originating primarily from the electrochemical reducting reaction of oxidized form electron mediator under negative potential that reaction generates.For this type of electrochemical test strip, dual-electrode pattern can be adopted to reduce the interference of ascorbic acid.Equally, the first work for target analytes electrode, survey electric current and be designated as I1, the second working electrode is blank electrode, survey electric current and be designated as I2, all add novel ascorbic acid oxidase at the first and second working electrode surfaces.In actual measurement, the all oxidized generation hydrogen peroxide of ascorbic acid of the first and second working electrode surfaces, under negative potential, all there is electroreduction to a certain degree in the hydrogen peroxide of the first and second electrode surfaces, generating portion just disturbs reduction current, by the first working electrode currents I1deduct the second working electrode currents I2obtain the electric current I (I=I after background correction current signal1-I2), this part can be eliminated and just disturb reduction current, improve the antijamming capability of electrochemical test strip Ascorbic Acid.Add novel ascorbic acid oxidase the negative interference of ascorbic acid is transformed into just disturbs, utilize the method for dual-electrode pattern background correction signal to reduce ascorbic acid to the interference of electrochemical test bar taking reduction current as detection signal.
In embodiments more provided by the invention, the enzymic catalytic reaction path of novel ascorbic acid oxidase is: L-AA and oxygen generate L-hydroascorbic acid and hydrogen peroxide under the catalysis of described ascorbic acid oxidase.
In embodiments more provided by the invention, the molecular weight of novel ascorbic acid oxidase is 130000, and isoelectric point is 9.0, Km constant is 4.9 × 10-4m.
In embodiments more provided by the invention, the biology enzyme corresponding with thing to be detected is glucose oxidase, or is beta-hydroxybutyric dehydrogenase and/or nicotinamide adenine dinucleotide, and electron mediator is oxidized form electron mediator;
The biology enzyme corresponding with thing to be detected is urate oxidase and/or peroxidase, or is cholesterol esterase and/or cholesterol oxidase, and electron mediator is reduced form electron mediator.
In embodiments more provided by the invention, oxidized form electron mediator is metal or noble metal complexes, or organic oxidation reduction amboceptor.
As preferably, metal or noble metal complexes are a kind of of chlorination six ammino ruthenium (III), Ferrocene and its derivative or the potassium ferricyanide or both above potpourris.
As preferably, organic oxidation reduction amboceptor is a kind of or both above potpourris of Synoptic model, benzoquinones, the how green B of phenol, methylene blue, meldola blue, toluidine blue, tetrathiafulvalene or Phen diketone.
Preferably, oxidisability electron mediator is the potassium ferricyanide or Synoptic model.
Preferably, reductibility electron mediator is potassium ferrocyanide.
As preferably, the mass ratio of microbe-derived ascorbic acid oxidase, the biology enzyme corresponding with thing to be detected and electron mediator is (0.1 ~ 10): (0.1 ~ 10): (1 ~ 5).
Preferably, the mass ratio of microbe-derived ascorbic acid oxidase, the biology enzyme corresponding with thing to be detected and electron mediator is 1:1:3.
As preferably, working electrode bioreacter of the present invention also comprises a kind of or both the above potpourris in bonding agent, surfactant or filling agent.
In embodiments more provided by the invention, bonding agent is hydroxyethyl cellulose.But invention adhesives kind is not defined in this, those skilled in the art think that feasible bonding agent is all within protection scope of the present invention.
In embodiments more provided by the invention, surfactant is Triton-100.But kinds of surfactants of the present invention is not defined in this, those skilled in the art think that feasible surfactant is all within protection scope of the present invention.
In embodiments more provided by the invention, filling agent is lactose.But filling agent kind of the present invention is not defined in this, those skilled in the art think that feasible filling agent is all within protection scope of the present invention.
As preferably, the mass ratio of microbe-derived ascorbic acid oxidase, the biology enzyme corresponding with thing to be detected, electron mediator, bonding agent, surfactant and filling agent is (0.1 ~ 10): (0.1 ~ 10): (1 ~ 5): (1 ~ 10): (0.1 ~ 1): (1 ~ 10).
Preferably, the mass ratio of microbe-derived ascorbic acid oxidase, the biology enzyme corresponding with thing to be detected, electron mediator, bonding agent, surfactant and filling agent is 1:1:3:5:0.2:5.
Present invention also offers a kind of electric pole type test-strips, comprise flaggy body 100 and the electrode being positioned at flaggy body 100 inside; One end of flaggy body 100 is collection terminal 20, and the other end is electrical contact end 10, and electrical contact end 10 can insert current-responsive analytical equipment;
Flaggy body 100 comprises the multi-layer insulation layer superposed successively, is followed successively by the first basic unit of basic unit 200, second 300, first upper strata 400 and the second upper strata 500 from bottom to up;
The face that first basic unit 200 contacts with the second basic unit 300 is provided with electrode, and electrode comprises the first electrode 11, second electrode 12 and the 3rd electrode 13; Each electrode is all connected with the first notch 44 of the first basic unit 200, and the first notch 44 is positioned at the end of the first basic unit 200 collection terminal;
The collection terminal place of the second basic unit 300 is provided with the through hole of answering with electrode pair, through hole comprises the first through hole 31, second through hole 32, third through-hole 33, first through hole 31 is corresponding with the first electrode 11, and the second through hole 32 is corresponding with the second electrode 12, and third through-hole 33 is corresponding with the 3rd electrode 13; The end of the second basic unit 300 collection terminal is provided with the second notch 54;
The end of the first upper strata 400 collection terminal is provided with sap cavity notch 41; First collection terminal both sides, upper strata 400 place is provided with import 91;
Second upper strata 500, second basic unit 300 forms sap cavity with sap cavity notch 41; The collection terminal place on the second upper strata 500 is provided with vent port 74, and vent port 74 communicates with sap cavity;
First notch 44 forms the slotted eye 34 communicated with sap cavity jointly with the second notch 54;
One or two in first electrode 11, second electrode 12 or the 3rd electrode 13 is working electrode, working electrode is provided with working electrode bioreacter provided by the invention.
As preferably, the biology enzyme corresponding with thing to be detected is glucose oxidase, or beta-hydroxybutyric dehydrogenase and/or nicotinamide adenine dinucleotide, and electric pole type test-strips adopts single working electrode pattern or dual-electrode pattern;
The biology enzyme corresponding with thing to be detected is urate oxidase and/or peroxidase, or cholesterol esterase and/or cholesterol oxidase, and electric pole type test-strips adopts dual-electrode pattern;
Dual-electrode pattern is with the first electrode (11) and the 3rd electrode (13) for working electrode, and the second electrode (12) is contrast electrode;
Single working electrode pattern is with the second electrode (12) for working electrode, and the first electrode (11) and the 3rd electrode (13) are contrast electrode.
In embodiments more provided by the invention, in the electrochemical test bar taking oxidation current as detection signal, its oxidation current is originating primarily from the electrochemical oxidation reactions of reduced form electron mediator under positive potential that reaction generates, for this type of electrochemical test strip adopting single working electrode, working electrode surface is provided with working electrode bioreacter of the present invention, contrast electrode is provided with contrast electrode bioreacter, working electrode bioreacter is compared with contrast electrode bioreacter, there is microbe-derived ascorbic acid oxidase, these two kinds of materials of biology enzyme, Ascorbic Acid Oxidation can be generated hydrogen peroxide by the novel ascorbic acid oxidase on working electrode, and hydrogen peroxide produce at electrode surface generation electro-oxidation reaction the degree just disturbed will much smaller than ascorbic acid, therefore can reduce or eliminate the interference of ascorbic acid to this type of electrochemical test strip.
Be the antijamming capability that a step improves examination bar, the electrochemical test strip of dual-electrode can be adopted, comprise the first working electrode and the second working electrode.First working electrode is target analytes electrode, working electrode bioreacter on it comprises microbe-derived ascorbic acid oxidase (N-ASO), the biology enzyme corresponding with thing to be detected, electron mediator, bonding agent, surfactant and filling agent, survey electric current and be designated as I1, the second working electrode is blank electrode, and the working electrode bioreacter on it comprises microbe-derived ascorbic acid oxidase (N-ASO), electron mediator, bonding agent, surfactant and filling agent, survey electric current and be designated as I2.Namely novel ascorbic acid oxidase is all added at the first and second working electrode surfaces.In actual measurement, the all oxidized generation hydrogen peroxide of ascorbic acid of the first and second working electrode surfaces, under positive potential, all there is electrooxidation to a certain degree in the hydrogen peroxide of the first and second electrode surfaces, generating portion just disturbs oxidation current, by the first working electrode currents I1deduct the second working electrode currents I2obtain the electric current I (I=I after background correction current signal1-I2), this part can be eliminated and just disturb oxidation current, improve the antijamming capability of electrochemical test strip Ascorbic Acid further.
In the electrochemical test bar taking reduction current as detection signal, its reduction current is originating primarily from the electrochemical reducting reaction of oxidized form electron mediator under negative potential that reaction generates.For this type of electrochemical test strip, dual-electrode pattern can be adopted to reduce the interference of ascorbic acid.Equally, the first work for target analytes electrode, survey electric current and be designated as I1, the second working electrode is blank electrode, survey electric current and be designated as I2, all add ascorbic acid oxidase at the first and second working electrode surfaces.In actual measurement, the all oxidized generation hydrogen peroxide of ascorbic acid of the first and second working electrode surfaces, under negative potential, all there is electroreduction to a certain degree in the hydrogen peroxide of the first and second electrode surfaces, generating portion just disturbs reduction current, by the first working electrode currents I1deduct the second working electrode currents I2obtain the electric current I (I=I after background correction current signal1-I2), this part can be eliminated and just disturb reduction current, improve the antijamming capability of electrochemical test strip Ascorbic Acid.Add novel ascorbic acid oxidase the negative interference of ascorbic acid is transformed into just disturbs, utilize the method for dual-electrode pattern background correction signal to reduce ascorbic acid to the interference of electrochemical test bar taking reduction current as detection signal.
Present invention also offers a kind of electrochemical detection method of non-diagnostic object, comprising:
Adopt electric pole type test-strips provided by the invention to detect the current signal of sample to be tested, obtain the concentration of thing to be detected.
The invention provides working electrode bioreacter and electric pole type test-strips.This working electrode bioreacter comprises microbe-derived ascorbic acid oxidase, the biology enzyme corresponding with thing to be detected, and electron mediator.The present invention at least has one of following advantage:
1, direct compound target is adopted to analyze the method for the single-minded enzyme of thing and novel ascorbic acid oxidase (N-ASO) to reduce the interference of ascorbic acid in the present invention, any interference (if do not affected the precision of examination bar, not reducing current-responsive sensitivity) is not produced on the enzyme reaction current responsing signal of the single-minded enzyme of target analytes;
2, electric pole type test-strips of the present invention is applicable to adopt single or multiple working electrode system, not only can reduce ascorbic acid to the interference of electrochemical test bar taking oxidation current as detection signal, also can reduce ascorbic acid to the interference of electrochemical test bar taking reduction current as detection signal.
Accompanying drawing explanation
Fig. 1 shows the structural representation of a kind of electrochemical test bar adopted in the embodiment of the present invention;
Fig. 2 shows the STRUCTURE DECOMPOSITION figure of a kind of electrochemical test bar adopted in the embodiment of the present invention;
Current-responsive figure when Fig. 3 shows that different operating electrode enzyme membrane component made glucose electrochemical test strip measures same concentration of glucose blood sample (200mg/dl).
Embodiment
The invention discloses working electrode bioreacter and electric pole type test-strips, those skilled in the art can use for reference present disclosure, and suitable improving technique parameter realizes.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are all deemed to be included in the present invention.Method of the present invention and application are described by preferred embodiment, related personnel obviously can not depart from content of the present invention, spirit and scope methods and applications as herein described are changed or suitably change with combination, realize and apply the technology of the present invention.
In working electrode bioreacter provided by the invention, electric pole type test-strips and electrochemical detection method, enzyme used, reagent, instrument etc. all can be buied by market.Ascorbic acid oxidase novel in the present invention is purchased from Japanese Tian Ye company, and itself and traditional ascorbic acid oxidase have following difference:
The Nature comparison * of the novel and traditional ascorbic acid oxidase of table 1
* Tian Ye company enzyme product handbook is selected from.
Below in conjunction with embodiment, set forth the present invention further:
The structure of embodiment 1 electrochemical test bar, composition and performance test
As shown in Figure 1, its STRUCTURE DECOMPOSITION schematic diagram as shown in Figure 2 for the schematic diagram of electric pole type test-strips.
The structural representation of a kind of electrochemical test bar that Fig. 1 adopts for electrochemical glucose sensor.This test-strips comprises flaggy body 100 and is positioned at the electrode of flaggy body 100 inside, and the two ends of flaggy body 100 are respectively collection terminal 20 and electrical contact end 10.Wherein, collection terminal 20 is for receiving fluid sample, the inside of collection terminal 20 is provided with the sap cavity as fluid sample room, the both sides (i.e. side import 91) of collection terminal 20 and front side (" front side " i.e. sensor body 100 is positioned at the end face of collection terminal 20) communicate with sap cavity respectively, and the bottom surface of collection terminal 20 is provided with the slotted eye 34 communicated with sap cavity.
The STRUCTURE DECOMPOSITION figure of a kind of electrochemical test bar that Fig. 2 adopts for electrochemical glucose sensor.Flaggy body 100 comprises the multi-layer insulation layer superposed successively, is followed successively by the first basic unit of basic unit 200, second 300, first upper strata 400 and the second upper strata 500 from bottom to up.
Wherein: the upper surface of the first basic unit 200 is provided with electrode (i.e. conductive coating), as working electrode, contrast electrode and the electric contact points that is connected with test instrumentation, in the present embodiment, the first basic unit 200 is provided with three electrodes (namely electrical contact end has three electrical pickofves), be respectively electrode 11, electrode 12 and electrode 13, each electrode is all connected with the first notch 44 (thus being connected with slotted eye 34).In addition, the leading flank of the first basic unit 200 is provided with notch 44;
The part that second basic unit 300 is positioned at collection terminal 20 is provided with the through hole of answering with electrode pair, so that a part for conductive coating is come out, for limiting electrode area and load chemical reagent, through hole in the present embodiment in the second basic unit 300 comprises the first through hole 31, second through hole 32, third through-hole 33, three through holes and three electrode one_to_one corresponding.In addition, the leading flank of the second basic unit 300 is provided with notch 54 (its position, shape and size are consistent with the first notch 44);
The end that first upper strata 400 is positioned at collection terminal 20 is provided with sap cavity notch 41; First collection terminal both sides, upper strata 400 place is provided with import 91;
Second upper strata 500 and the second basic unit 300 part corresponding with sap cavity notch 41 forms sap cavity with sap cavity notch 41, and the second upper strata 500 is positioned at collection terminal 20 part and is provided with the vent port 74 communicated with sap cavity;
First notch 44 forms the slotted eye 34 communicated with sap cavity jointly with the second notch 54;
Get the first basic unit 200, in the first surface mount second basic unit of basic unit 200 300,0.15 microlitre working electrode biological respinse film liquid is dripped respectively again at working electrode surface, 0.15 microlitre contrast electrode biological respinse film liquid is dripped on contrast electrode surface, by drip have the first basic unit 200 of biological respinse film liquid and the second basic unit 300 to be placed in 35 DEG C at dry 5min, finally paste the first upper strata 400 and the second upper strata 500 successively on the second basic unit 300 surface, obtain electric pole type test-strips.
The research of single working electrode model comparision is adopted to add impact on electrochemical glucose sensor performance after ASO and N-ASO.Under single working electrode pattern, electrode 12 is working electrode, and electrode 11 and electrode 13 are contrast electrode.Enzyme membrane (biological respinse film) component on working electrode 12 surface is divided into following three kinds of situations:
A: be coated with glucose oxidase, novel ascorbic acid oxidase (N-ASO, 216U/mg, wild purchased from Japanese sky), the oxidized form electron mediator potassium ferricyanide, macromolecule urge stick hydroxyethyl cellulose, surfactant Triton-100 and filling agent lactose;
B: be coated with glucose oxidase, traditional ascorbic acid oxidase (ASO, 275U/mg, wild purchased from Japanese sky), the oxidized form electron mediator potassium ferricyanide, macromolecule urge stick hydroxyethyl cellulose, surfactant Triton-100 and filling agent lactose;
C: be coated with glucose oxidase, the oxidized form electron mediator potassium ferricyanide, macromolecule urge stick hydroxyethyl cellulose, surfactant Triton-100 and filling agent lactose.
Specifically as shown in table 2.
Table 2 working electrode reaction enzyme membrane formula of liquid
Electrode 11 and 13 is coated with the oxidized form electron mediator potassium ferricyanide, macromolecule urgees stick hydroxyethyl cellulose, surfactant Triton-100 and filling agent lactose, specifically as shown in table 3.
Table 3 contrast electrode reaction enzyme membrane
Electrochemical test bar and constant potential measuring instrument are linked (CHI electrochemical workstation, Shanghai occasion China), apply the positive voltage 0.3V relative to contrast electrode 11 or contrast electrode 13 on working electrode 12 surface, record the electric current (5s value) relevant to concentration of glucose on working electrode 12 surface.
Under single working electrode pattern, the specific experiment step of comparative studies different operating electrode enzyme membrane component made glucose electrochemical test bar test performance is as follows:
(1) with the fresh venous of normal person for matrix, configure the blood sample (concentration is respectively 70mg/dl, 100mg/dl, 200mg/dl, 400mg/dl and 550mg/dl) of different concentration of glucose (C);
(2) CHI electrochemical workstation is adopted to measure three kinds of glucose electrochemical test bars respectively to the current-responsive (I) of different concentration of glucose blood sample, by concentration of glucose (C) to electric current (I) mapping, the linear equation obtaining different glucose electrochemical test bar is respectively C=a*I+b;
(3) calculate the test performance of three kinds of glucose electrochemical test bars respectively, comprise the measurement mean value of different concentration of glucose, standard deviation, CV, the response sensitivity of examination bar and the range of linearity.
Fig. 3 is the current-responsive figure of different operating electrode enzyme membrane component made glucose electrochemical test strip when measuring same concentration of glucose blood sample (200mg/dl).As can be seen from Figure 3, and do not add compared with anti-interference enzyme, after adding ASO, examination bar reduces the current-responsive of glucose, and the precision of test is poor, and adds N-ASO and have no significant effect the current-responsive of measurement and precision.
Table 4 gives various performance parameters during different operating electrode enzyme membrane component made glucose electrochemical test strip measurement glucose blood sample.
The test performance of the made glucose electrochemical test bar of table 4 different operating electrode enzyme membrane component
As known from Table 4, the CV of made examination bar, response sensitivity and the range of linearity and not add the measurement performance of anti-interference enzyme made examination bar close add N-ASO in working electrode enzyme membrane after, and the CV of made examination bar increases after adding ASO in working electrode enzyme membrane, response sensitivity reduces, and the range of linearity also narrows.After the above results illustrates in working electrode enzyme membrane and adds N-ASO on the enzyme reaction performance of glucose itself substantially without affecting.
The anti-interference test of embodiment 2 electrochemical glucose sensor
The present embodiment is reduce ascorbic acid to the interference of electrochemical glucose sensor taking oxidation current as detection signal.The electrochemical test bar structure that the present embodiment adopts is identical with embodiment 1.
(1) single working electrode pattern
When adopting single working electrode pattern, electrode 12 is working electrode, and electrode 11 and electrode 13 are contrast electrode.Enzyme membrane (biological respinse film) component on electrode 12 surface is identical with the enzyme membrane component in embodiment 1, applies A, B and C tri-kinds of enzyme membrane components respectively, specifically as shown in table 2 in embodiment 1.Electrode 11 and 13 is coated with the oxidized form electron mediator potassium ferricyanide, macromolecule urgees stick hydroxyethyl cellulose and filling agent lactose, with contrast electrode enzyme membrane component identical (table 3) in embodiment 1.Electrochemical test bar and constant potential measuring instrument are linked (CHI electrochemical workstation, Shanghai occasion China), apply the positive voltage 0.3V relative to contrast electrode 11 or contrast electrode 13 on working electrode 12 surface, record the electric current (5s value) relevant to concentration of glucose on working electrode 12 surface.Investigate electrode 12 surface respectively and be coated with N-ASO and ASO, and try the interference free performance of bar Ascorbic Acid during uncoated anti-interference enzyme.In actual measurement, ascorbic acid can produce measured value and just disturb.Glucose measurement result being greater than 10% (for concentration of glucose > 100mg/dl) or 10mg/dl (for concentration of glucose < 100mg/dl) exports change and is defined as remarkable interference.
Under single working electrode pattern, the concrete steps of ascorbic acid interference experiment are as follows:
(1) with the fresh venous of normal person for matrix, configure the blood sample (as concentration is respectively 70mg/dl, 100mg/dl, 200mg/dl, 300mg/dl and 550mg/dl) of different concentration of glucose (C);
(2) CHI electrochemical workstation is adopted to measure the current-responsive (I) of glucose sugar electrochemical test bar working electrode surface under different concentration of glucose blood sample, by glucose sugar concentration (C) to electric current (I) mapping, the linear equation obtained between C and I is C=a*I+b;
(3) to certain concentration of glucose (C1) (as 100mg/dl or 300mg/dl) blood sample in add the ascorbic acid of variable concentrations respectively, after shaking up balance, adopt CHI electrochemical workstation to measure glucose sugar electrochemical test bar to the current-responsive (I adding blood sample after ascorbic acidinterference), then by this Iinterferencesubstitute into step 2 gained linear equation, after ascorbic acid chaff interference must be added survey concentration of glucose (C2), calculate C2with C1between measured deviation, obtain ascorbic acid concentrations measurement result being produced under certain concentration of glucose to significantly interference.
Add N-ASO and ASO respectively under single working electrode pattern, and the antijamming capability of glucose test strip Ascorbic Acid not adding anti-interference enzyme is in table 5.
Respectively N-ASO and ASO under the single working electrode pattern of table 5, and the antijamming capability of electrochemical glucose sensor (oxidation current is signal) Ascorbic Acid not adding anti-interference enzyme
From the test findings of table 5, do not significantly improve the antijamming capability of examination bar after adding ASO, but the antijamming capability trying bar after adding N-ASO significantly improves.
(2) dual-electrode pattern
When adopting dual-electrode pattern, electrode 11 is the first working electrode, and electrode 13 is the second working electrode, and electrode 12 is contrast electrode.Enzyme membrane (biological respinse film) component on electrode 11 surface is identical with the enzyme membrane component in embodiment 1, applies A, B and C tri-kinds of enzyme membrane components respectively, specifically as shown in table 2 in embodiment 1.Enzyme membrane (biological respinse film) component on electrode 13 surface is also divided into A, B and C tri-kinds of enzyme membrane components, specifically as shown in table 6.The pattern that electrode 11 and electrode 13 are combined into dual-electrode is as follows: enzyme membrane component A (electrode 11)-A (electrode 13), enzyme membrane B component (electrode 11)-B (electrode 13), enzyme membrane component C (electrode 11)-C (electrode 13).
Table 6 second working electrode reaction enzyme membrane formula of liquid
| Component | A | B | C |
| Novel ascorbic acid oxidase (N-ASO) | 1％ | - | - |
| Tradition ascorbic acid oxidase (ASO) | - | 1％ | - |
| Hydroxyethyl cellulose | 5％ | 5％ | 5％ |
| Triton-100 | 0.2％ | 0.2％ | 0.2％ |
| The potassium ferricyanide | 3％ | 3％ | 3％ |
| Lactose | 5％ | 5％ | 5％ |
| Water | 85.8％ | 85.8％ | 86.8％ |
Electrode 12 is coated with the oxidized form electron mediator potassium ferricyanide, macromolecule urgees stick hydroxyethyl cellulose and filling agent lactose, with contrast electrode enzyme membrane component identical (table 3) in embodiment 1.Electrochemical test bar and constant potential measuring instrument are linked (CHI electrochemical workstation, Shanghai occasion China), apply the positive voltage 0.3V relative to contrast electrode 12 at working electrode 11 and electrode 13 surface, record the electric current I relevant to concentration of glucose on the first working electrode 11 surface1, the second working electrode 13 surface measurement background interference electric current I2.By the first working electrode currents I1deduct the second working electrode currents I2obtain the electric current I (I=I after background correction current signal1-I2), this electric current is corresponding with concentration of glucose.Investigate electrode 11 respectively and electrode 13 surface is coated with N-ASO and ASO, and try the interference free performance of bar Ascorbic Acid during uncoated anti-interference enzyme.
Under dual-electrode pattern, the concrete steps of ascorbic acid interference experiment are as follows:
(1) with the fresh venous of normal person for matrix, configure the blood sample (as concentration is respectively 70mg/dl, 100mg/dl, 200mg/dl, 300mg/dl and 550mg/dl) of different concentration of glucose (C);
(2) under adopting CHI electrochemical workstation to measure different concentration of glucose blood sample, glucose sugar electrochemical test bar first working electrode currents responds (I1) and the second working electrode (i.e. background electrode) current-responsive (I2), by the first working electrode currents I1deduct the second working electrode currents I2obtain the electric current I (I=I after background correction current signal1-I2), this electric current is corresponding with concentration of glucose, and by glucose sugar concentration (C) to electric current (I) mapping, the linear equation obtained between C and I is C=a*I+b;
(3) to certain concentration of glucose (C1) (100mg/dl or 300mg/dl) blood sample in add the ascorbic acid of variable concentrations respectively, after shaking up balance, adopt CHI electrochemical workstation to measure glucose sugar electrochemical test bar to the current-responsive I adding blood sample after ascorbic acid1 interferenceand I2 interference, obtain the I after background correction signalinterference(Iinterference=I1 interference-I2 interference), then by this Iinterferencesubstitute into step 2 gained linear equation, after ascorbic acid chaff interference must be added survey concentration of glucose (C2), calculate C2with C1between measured deviation, obtain ascorbic acid concentrations measurement result being produced under certain concentration of glucose to significantly interference.
Add N-ASO and ASO respectively under dual-electrode pattern, and the antijamming capability of glucose test strip Ascorbic Acid not adding anti-interference enzyme is in table 7.
Add N-ASO and ASO respectively under table 7 dual-electrode pattern, and do not add the antijamming capability of electrochemical glucose sensor (oxidation current is signal) Ascorbic Acid of anti-interference enzyme
As seen from the results in Table 7, after using the inventive method to add N-ASO, the antijamming capability of test-strips significantly improves, and shows, in the glucose sugar electrochemical test bar taking oxidation current as detection signal, to use method of the present invention can effectively reduce the impact of ascorbic acid.
The anti-interference test of embodiment 3 blood ketone electrochemical sensor
The present embodiment is reduce ascorbic acid to the interference of the blood ketone electrochemical sensor being detection signal with oxidation current electric current.The electrochemical test bar structure that the present embodiment adopts is identical with embodiment 1 scheme.
(1) single working electrode pattern
When adopting single working electrode pattern, electrode 12 is working electrode, and electrode 11 and electrode 13 are contrast electrode.Enzyme membrane (biological respinse film) component on working electrode 12 surface is divided into following three kinds of situations:
A: be coated with beta-hydroxybutyric dehydrogenase, nicotinamide adenine dinucleotide (NAD+), novel ascorbic acid oxidase (N-ASO, 216U/mg, wild purchased from Japanese sky), oxidized form electron mediator Synoptic model, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose;
B: be coated with beta-hydroxybutyric dehydrogenase, nicotinamide adenine dinucleotide (NAD+), traditional ascorbic acid oxidase (ASO, 275U/mg, wild purchased from Japanese sky), oxidized form electron mediator Synoptic model, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose;
C: be coated with nicotinamide adenine dinucleotide (NAD+), oxidized form electron mediator Synoptic model, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose.
Specifically as shown in table 8.
Table 8 working electrode reaction enzyme membrane
Electrode 11 and electrode 13 are coated with nicotinamide adenine dinucleotide (NAD+), oxidized form electron mediator Synoptic model, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose, specifically as shown in table 9.
Table 9 contrast electrode reaction enzyme membrane
| Component | Consumption |
| NAD+ | 3％ |
| Hydroxyethyl cellulose | 5％ |
| Triton-100 | 0.2％ |
| Synoptic model | 3％ |
| Lactose | 5％ |
| Water | 83.8％ |
Electrochemical test bar and constant potential measuring instrument are linked (CHI electrochemical workstation, Shanghai occasion China), apply the positive voltage 0.3V relative to contrast electrode 11 or contrast electrode 13 on working electrode 12 surface, record with beta-hydroxybutyric acid concentration dependent electric current (20s value) on working electrode 12 surface.Investigate electrode 12 surface respectively and be coated with N-ASO and ASO, and try the interference free performance of bar Ascorbic Acid during uncoated anti-interference enzyme.In actual measurement, ascorbic acid can produce measured value and just disturb.Beta-hydroxybutyric acid measurement result being greater than 10% (for beta-hydroxybutyric acid concentration > 0.3mM) or 0.03mM (for beta-hydroxybutyric acid concentration < 0.3mM) exports change and is defined as remarkable interference.Single electrode mode of operation in the step of ascorbic acid interference experiment and embodiment 2 is similar.Table 10 for adding N-ASO and ASO respectively under single working electrode pattern, and does not add the antijamming capability of blood ketone test-strips Ascorbic Acid of anti-interference enzyme.
Add N-ASO and ASO respectively under the single working electrode pattern of table 10, and do not add the antijamming capability of blood ketone electrochemical sensor (oxidation current is signal) Ascorbic Acid of anti-interference enzyme
As seen from the results in Table 10, do not significantly improve the antijamming capability of examination bar after adding ASO, after adding N-ASO, the antijamming capability of test-strips significantly improves.
(2) dual-electrode pattern
When adopting dual-electrode pattern, electrode 11 is the first working electrode, and electrode 13 is the second working electrode, and electrode 12 is contrast electrode.Enzyme membrane (biological respinse film) component on electrode 11 surface is identical with the enzyme membrane component under single working electrode pattern, applies A, B and C tri-kinds of enzyme membrane components respectively, specifically as shown in table 8.Enzyme membrane (biological respinse film) component on electrode 13 surface is also divided into A, B and C tri-kinds of enzyme membrane components, specifically as shown in table 11.The pattern that electrode 11 and electrode 13 are combined into dual-electrode is as follows: enzyme membrane component A (electrode 11)-A (electrode 13), enzyme membrane B component (electrode 11)-B (electrode 13), enzyme membrane component C (electrode 11)-C (electrode 13).
Table 11 second working electrode reaction enzyme membrane
Electrode 12 applies nicotinamide adenine dinucleotide (NAD+), oxidized form electron mediator Synoptic model, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose, identical, specifically as shown in table 9 with contrast electrode enzyme membrane component under single electrode mode of operation.
Electrochemical test bar and constant potential measuring instrument are linked (CHI electrochemical workstation, Shanghai occasion China), apply the positive voltage 0.3V relative to contrast electrode 12 at working electrode 11 and electrode 13 surface, record the concentration dependent electric current I with beta-hydroxybutyric acid on the first working electrode 11 surface1, the second working electrode 13 surface measurement background interference electric current I2.By the first working electrode currents I1deduct the second working electrode currents I2obtain the electric current I (I=I after background correction current signal1-I2), this electric current and beta-hydroxybutyric acid relative concentration should.Investigate electrode 11 respectively and electrode 13 surface is coated with N-ASO and ASO, and try the interference free performance of bar Ascorbic Acid during uncoated anti-interference enzyme.Bipolar electrode mode of operation in the step of ascorbic acid interference experiment and embodiment 2 is similar.Table 12 for adding N-ASO and ASO respectively under dual-electrode pattern, and does not add the antijamming capability of blood ketone test-strips Ascorbic Acid of anti-interference enzyme.
Add N-ASO and ASO respectively under table 12 dual-electrode pattern, and do not add the antijamming capability of blood ketone electrochemical sensor (oxidation current is signal) Ascorbic Acid of anti-interference enzyme
From table 12 result, after using the inventive method to add N-ASO, the antijamming capability of blood ketone test-strips significantly improves, show, in the blood ketone electrochemical test bar taking oxidation current as detection signal, to use method of the present invention can effectively reduce the impact of ascorbic acid.
The anti-interference test of embodiment 4 uric acid electrochemical sensor
The present embodiment is reduce ascorbic acid to the interference of uric acid electrochemical sensor taking reduction current as detection signal.The electrochemical test bar structure that the present embodiment adopts is identical with embodiment 1.
Uric acid electrochemical sensor adopts dual-electrode pattern, and electrode 11 is the first working electrode, and electrode 13 is the second working electrode, and electrode 12 is contrast electrode.Enzyme membrane (biological respinse film) component on working electrode 11 surface is divided into following three kinds of situations:
A: be coated with urate oxidase, novel ascorbic acid oxidase (N-ASO, 216U/mg, wild purchased from Japanese sky), peroxidase, reduced form electron mediator potassium ferrocyanide, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose;
B: be coated with urate oxidase, traditional ascorbic acid oxidase (ASO, 275U/mg, wild purchased from Japanese sky), peroxidase, reduced form electron mediator potassium ferrocyanide, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose;
C: be coated with urate oxidase, peroxidase, reduced form electron mediator potassium ferrocyanide, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose.
Specifically as shown in table 13.
Table 13 first working electrode reaction enzyme membrane
Enzyme membrane (biological respinse film) component on electrode 13 surface is also divided into A, B and C tri-kinds of enzyme membrane components, specifically as shown in table 14.The pattern that electrode 11 and electrode 13 are combined into dual-electrode is as follows: enzyme membrane component A (electrode 11)-A (electrode 13), enzyme membrane B component (electrode 11)-B (electrode 13), enzyme membrane component C (electrode 11)-C (electrode 13).
Table 14 second working electrode reaction enzyme membrane
Contrast electrode 12 is coated with reduced form electron mediator potassium ferrocyanide, macromolecule urgees stick hydroxyethyl cellulose and filling agent lactose, specifically as shown in Table 15.
Table 15 contrast electrode reaction enzyme membrane
| Component | Consumption |
| Hydroxyethyl cellulose | 5％ |
| Triton-100 | 0.2％ |
| Potassium ferrocyanide | 3％ |
| Lactose | 5％ |
| Water | 86.8％ |
Electrochemical test bar and constant potential measuring instrument are linked (CHI electrochemical workstation, Shanghai occasion China), first working electrode 11 and the second working electrode 13 apply the negative voltage-0.15V relative to contrast electrode 12 simultaneously, when electrode 11 and electrode 13 surface are coated with N-ASO, record the electric current I relevant to uric acid and ascorbic acid concentrations on the first working electrode 11 surface1(15s value), the second working electrode 13 surface measurement and ascorbic acid concentrations relevant interference electric current I2(15s value).By the first working electrode currents I1deduct the second working electrode currents I2electric current I (the I=I after ascorbic acid current signal must be deducted1-I2), this electric current is corresponding with uric acid concentration.When not adding N-ASO, ascorbic acid produces negative interference to test result, and after adding N-ASO, the interference of ascorbic acid to test result becomes just to be disturbed.Investigate electrode 11 respectively and electrode 13 surface is coated with N-ASO and ASO, and try the interference free performance of bar Ascorbic Acid during uncoated anti-interference enzyme.Measurement result is greater than ± uric acid of 10% (for uric acid concentration > 0.3mM) or ± 0.03mM (for uric acid concentration < 0.3mM) exports change and is defined as remarkable interference.Bipolar electrode mode of operation in the step of ascorbic acid interference experiment and the second embodiment is similar.Table 16 for adding N-ASO and ASO respectively under dual-electrode pattern, and does not add the antijamming capability of uric acid test strip Ascorbic Acid of anti-interference enzyme.
Add N-ASO and ASO respectively under table 16 dual-electrode pattern, and do not add the antijamming capability of uric acid electrochemical sensor (reduction current is signal) Ascorbic Acid of anti-interference enzyme
From table 16 result, after using the inventive method to add N-ASO, the antijamming capability of test-strips significantly improves, and shows, in the uric acid electrochemical test bar taking reduction current as detection signal, to use method of the present invention can effectively reduce the impact of ascorbic acid.
The anti-interference test of embodiment 5 blood fat electrochemical sensor
The present embodiment is reduce ascorbic acid to the interference of blood fat electrochemical sensor taking reduction current as detection signal.The electrochemical test bar structure that the present embodiment adopts is identical with embodiment 1.
Blood fat electrochemical sensor adopts dual-electrode pattern, and electrode 11 is the first working electrode, and electrode 13 is the second working electrode, and electrode 12 is contrast electrode.Enzyme membrane (biological respinse film) component on working electrode 11 surface is divided into following three kinds of situations:
A: be coated with cholesterol esterase, cholesterol oxidase, novel ascorbic acid oxidase (N-ASO, 216U/mg, wild purchased from Japanese sky), peroxidase, reduced form electron mediator potassium ferrocyanide, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose;
B: cholesterol esterase, cholesterol oxidase, traditional ascorbic acid oxidase (ASO, 275U/mg, wild purchased from Japanese sky), peroxidase, reduced form electron mediator potassium ferrocyanide, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose;
C: be coated with cholesterol esterase, cholesterol oxidase, peroxidase, reduced form electron mediator potassium ferrocyanide, macromolecule urge stick hydroxyethyl cellulose and filling agent lactose.
Specifically shown in table 17.
Table 17 first working electrode reaction enzyme membrane
Enzyme membrane (biological respinse film) component on electrode 13 surface is also divided into A, B and C tri-kinds of enzyme membrane components, identical, specifically as shown in table 14 with the second working electrode enzyme membrane component in embodiment 4.The pattern that electrode 11 and electrode 13 are combined into dual-electrode is as follows: enzyme membrane component A (electrode 11)-A (electrode 13), enzyme membrane B component (electrode 11)-B (electrode 13), enzyme membrane component C (electrode 11)-C (electrode 13).
Contrast electrode 12 is coated with reduced form electron mediator potassium ferrocyanide, macromolecule urgees stick hydroxyethyl cellulose and filling agent lactose, identical, specifically as shown in Table 15 with contrast electrode enzyme membrane component in embodiment 4.
Electrochemical test bar and constant potential measuring instrument are linked (CHI electrochemical workstation, Shanghai occasion China), first working electrode 11 and the second working electrode 13 apply the negative voltage-0.15V relative to contrast electrode 12 simultaneously, when electrode 11 and electrode 13 surface are coated with N-ASO, record the electric current I relevant to cholesterol and ascorbic acid concentrations on the first working electrode 11 surface1(50s value), the second working electrode 13 surface measurement and ascorbic acid concentrations relevant interference electric current I2(50s value).By the first working electrode currents I1deduct the second working electrode currents I2electric current I (the I=I after ascorbic acid current signal must be deducted1-I2), this electric current is corresponding with cholesterol concentration.When not adding N-ASO, ascorbic acid produces negative interference to test result, and after adding N-ASO, the interference of ascorbic acid to test result becomes just to be disturbed.Investigate electrode 11 respectively and electrode 13 surface is coated with N-ASO and ASO, and try the interference free performance of bar Ascorbic Acid during uncoated anti-interference enzyme.Measurement result is greater than ± cholesterol of 10% (for cholesterol concentration > 200mg/dl) or ± 20mg/dl (for cholesterol concentration < 200mg/dl) exports change and is defined as remarkable interference.Bipolar electrode mode of operation in the step of ascorbic acid interference experiment and the second embodiment is similar.Table 18 for adding N-ASO and ASO respectively under dual-electrode pattern, and does not add the antijamming capability of blood fat electrochemical test bar Ascorbic Acid of anti-interference enzyme.
Add N-ASO and ASO respectively under table 18 dual-electrode pattern, and do not add the antijamming capability of blood fat electrochemical sensor (reduction current is signal) Ascorbic Acid of anti-interference enzyme
From table 18 result, after using the inventive method to add N-ASO, the antijamming capability of test-strips significantly improves, and shows, in the blood fat electrochemical test bar taking reduction current as detection signal, to use method of the present invention can effectively reduce the impact of ascorbic acid.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.