技术领域technical field
本发明涉及一种运动训练调整的方法，特别涉及一种藉由经皮微针感测器量测人体中乳酸浓度以用于运动训练调整的方法。The invention relates to a method for adjusting sports training, in particular to a method for measuring the concentration of lactic acid in a human body by means of a percutaneous microneedle sensor for adjusting sports training.
背景技术Background technique
运动中主要使用醣类做为能量来源，醣类产生能量分为有氧及无氧两种方式，醣类在无氧状况下分解，除了会产生能量外，也会产生乳酸。在运动强度较低时，主要使用有氧的方式产生能量，无氧参与较少，因此乳酸产生的速度不高，人体可以很容易代谢掉，所以乳酸不会累积在身体及血液中。随着运动强度的增加，无氧能量使用比率提高，乳酸产生的速度也随之增快，人体清除乳酸的速度会逐渐赶不上乳酸产生的速度，因此乳酸就会开始慢慢在身体中堆积，于此运动强度下，人体中的乳酸值被称为乳酸阈值(LactateThreshold)。于有氧代谢和无氧代谢之间的乳酸阈值的时点上，人体中的乳酸水平会保持平衡。假如运动员的乳酸阈值与相关的心跳率为已知，即可根据以上数据最适化对该运动员的训练。Carbohydrates are mainly used as energy sources during exercise. Carbohydrates produce energy in two ways: aerobic and anaerobic. Carbohydrates are decomposed under anaerobic conditions. In addition to producing energy, lactic acid will also be produced. When the exercise intensity is low, aerobic methods are mainly used to generate energy, and anaerobic methods are less involved. Therefore, the production rate of lactic acid is not high, and the human body can easily metabolize it, so lactic acid will not accumulate in the body and blood. As the exercise intensity increases, the rate of anaerobic energy use increases, and the rate of lactic acid production also increases. The body's rate of clearing lactic acid will gradually fail to catch up with the rate of lactic acid production, so lactic acid will begin to slowly accumulate in the body. Under this exercise intensity, the lactic acid value in the human body is called the lactic acid threshold (Lactate Threshold). At the point of lactate threshold between aerobic and anaerobic metabolism, the lactate level in the body will remain in balance. Given an athlete's lactate threshold and associated heart rate is known, training can be optimized for that athlete based on the above data.
乳酸是组织氧合(oxygenation)最重要的生物标记，因此对体育，卫生保健的应用极为重要。乳酸浓度提供无氧阈的信息，此信息对制订耐力运动培训计划是非常重要的。目前乳酸量测装置的商业利益驱动来自于运动，健身，奶制品和国防工业。Lactic acid is the most important biomarker of tissue oxygenation, so it is extremely important for sports and health care applications. Lactate concentration provides information on the anaerobic threshold, which is important in developing an endurance exercise training program. Current commercial interest in lactate measuring devices is driven by the sports, fitness, dairy and defense industries.
静止休息的正常血乳酸浓度在0.5-2mM的范围内，然而在剧烈运动时达到无氧阈或受伤引发失血性休克的情况下，细胞供氧量变得有限时，乳酸会迅速增加。测量乳酸有助于识别疲劳反应(对运动员，其他体育人士和士兵尤其重要)和设计个人化的训练流程给运动员。乳酸测量也对许多重症监护的情况下具有临床意义，例如，乳酸性酸中毒，尤其体现在休克状态。The normal blood lactate concentration at rest is in the range of 0.5-2mM. However, when the anaerobic threshold is reached during strenuous exercise or in the case of hemorrhagic shock caused by injury, when the oxygen supply to cells becomes limited, lactate will increase rapidly. Measuring lactate can help identify fatigue responses (especially important for athletes, other athletes, and soldiers) and design individualized training routines for athletes. Lactate measurement is also clinically relevant in many critical care situations, for example, lactic acidosis, especially in states of shock.
有运动生理学者认为运动中及运动后血乳酸的变化，是骨骼肌等组织中乳酸生成速率，进入血液的速率和血液中乳酸消失速率之间平衡的表现。因此，运动强度、持续时间，各组织器官间的代谢机能，都和血乳酸的浓度有关，在运动中和运动后，可以连续检测血乳酸來了解运动时人体的影响及其规律。血乳酸的测试在运动训練上的运用，训練的内容是一个相当复杂的工作，为了要提供一个较完善的训練课程和训練计画，教練通常需要找出一个对运动员最适当的运动量以及刺激，乳酸在血中浓度，安静值约1mM，可作为判断运动强度的依据，血中乳酸浓度达4.0mM时，可作为判断无氧阈值。先前文献指出，做动态激烈的运动时，血液中乳酸浓度有高至30μM/g。另有文献指出，当肌肉内乳酸浓度达到20-25μM/g时，使人会完全疲劳而无法运动。Some exercise physiologists believe that the change of blood lactic acid during and after exercise is a manifestation of the balance between the production rate of lactic acid in tissues such as skeletal muscle, the rate of entering the blood and the rate of disappearance of lactic acid in the blood. Therefore, the exercise intensity, duration, and the metabolic functions of various tissues and organs are all related to the concentration of blood lactic acid. During and after exercise, blood lactic acid can be continuously detected to understand the impact and rules of the human body during exercise. The application of blood lactic acid test in sports training is quite complicated. In order to provide a more complete training course and training plan, the coach usually needs to find out the most suitable one for the athlete. The amount of exercise and stimulation, the concentration of lactic acid in the blood, the rest value is about 1mM, which can be used as the basis for judging the intensity of exercise, and when the concentration of lactic acid in the blood reaches 4.0mM, it can be used as the threshold for judging the anaerobic threshold. Previous literature pointed out that when doing dynamic and intense exercise, the concentration of lactic acid in the blood can be as high as 30 μM/g. Another literature pointed out that when the intramuscular lactic acid concentration reaches 20-25μM/g, people will be completely fatigued and unable to exercise.
又有文献指出运动中乳酸产生的主要因素是：一、运动的负荷强度；二、參与运动的肌肉量；三、运动持续的时间。乳酸产生的浓度多寡，则与运动过程中有氧(aerobic)和无氧(anaerobic)系统能量代谢的百分比有关。不同的能量系统输出，对于人体运动时血乳酸值影响其浓度的变化。以磷酸系统为主要供给能量来源时血乳酸浓度较少，一般来说不超过4mM；若以醣酵解系统为主要供应能量来源时，可以高达15mM；以有氧能量系统为主要供应能量时在4mM。因此在不同强度的训练后，血乳酸的堆积情形有显著的差异性。乳酸堆积具有两种意义，一种是因为加速醣酵解的作用，产生大量的能量以供肌肉收缩使用；另一是表示肌肉中酸化的程度提高，原先的有氧代谢系统功能不敷使用，全赖无氧醣酵解作用来提供能量。另外，有运动生理学者认为在稳定狀态下，血乳酸堆积的情形可以作为判断运动负荷的生物参数之一。There are also literatures pointing out that the main factors of lactic acid production during exercise are: 1. the load intensity of the exercise; 2. the muscle mass involved in the exercise; 3. the duration of the exercise. The concentration of lactic acid produced is related to the percentage of energy metabolism in the aerobic and anaerobic systems during exercise. Different energy system outputs affect the change of blood lactic acid concentration during human exercise. When the phosphate system is used as the main energy supply source, the blood lactic acid concentration is low, generally not exceeding 4mM; if the glycolysis system is used as the main energy supply source, it can be as high as 15mM; 4mM. Therefore, after different intensities of training, there are significant differences in the accumulation of blood lactic acid. The accumulation of lactic acid has two meanings. One is because of the accelerated glycolysis, which produces a large amount of energy for muscle contraction; It relies entirely on anaerobic glycolysis to provide energy. In addition, some exercise physiologists believe that in a steady state, blood lactic acid accumulation can be used as one of the biological parameters for judging exercise load.
乳酸堆积的最常见的原因是缺氧(hypoxia)(低组织和血液氧合)。实验室外测定乳酸浓度的标准方法是离散的血液取样和透过乳酸试纸来测量。然而，这对动态变化或预测信息帮助不大，并可能导致缺少一个精确的浓度重建。离散采样实际上不方便于耐力活动的情况下进行。此外，无法直接对结果作解释。The most common cause of lactic acid buildup is hypoxia (low tissue and blood oxygenation). The standard method of determining lactate concentrations outside the laboratory is discrete blood sampling and measurement through lactate strips. However, this does not help much with dynamic or predictive information and may result in a lack of an accurate concentration reconstruction. Discrete sampling is practically inconvenient for endurance activities. Furthermore, the results cannot be directly interpreted.
直接植入血管的乳酸量测装置不是一个实用的选择，原因是会造成血栓和栓塞的风险，引发通过血液/乳酸量测装置之间的相互作用，并让通过局部的血流产生动力学变化。经皮组织液测量提供一个更安全的选项，可将任何不利影响局部化。而且组织中的乳酸测量，也对局部的氧气供应可以提供更多的相关信息，从而检测局部组织缺氧，而不是通过血液分析所获得身体的平均值。Lactate measuring devices implanted directly into blood vessels are not a practical option due to the risk of thrombus and embolism, induced through-blood/lactate-measuring-device interactions, and dynamic changes in blood flow through the region . Percutaneous interstitial fluid measurements offer a safer option that localizes any adverse effects. Moreover, lactate measurements in tissues can provide more relevant information on local oxygen supply, thereby detecting local tissue hypoxia, than the average value of the body obtained through blood analysis.
使用乳酸量测装置量测乳酸值，是于运动员在不同运动强度时，采血做分析。医护人员采样血液的方法，多半采扎针并穿破角质层，来抽取血液以进行分析检测，然而此种破坏皮肤表层的取样方法，除了容易使运动员感觉疼痛，进而萌生排斥感外，皮肤表层的大量微生物，也容易在皮肤表层遭破坏的情况下，进入人体进而感染。为了降低感染的风险，需要高的卫生标准，因而使此方法变得繁复及昂贵。The lactic acid measurement device is used to measure the lactic acid value, which is to collect blood for analysis when athletes perform different exercise intensities. Most of the blood sampling methods used by medical personnel are to prick needles and puncture the cuticle to draw blood for analysis and testing. A large number of microorganisms can easily enter the human body and cause infection when the surface of the skin is damaged. To reduce the risk of infection, high hygiene standards are required, making this method complicated and expensive.
另外，一般非侵入式量测乳酸的方法是逐渐风行使用在运动医学的康科尼测试(Conconi test)。于此方法，受测者在400公尺的运动跑道上，前200公尺以一预定速度跑步，200公尺之后受测者分段增加节奏，例如分别0.5km/h。在运动跑道每一200公尺标记处，受测者于分别环行运动跑道之后，注意他当时的心跳率，并喊出数据给助手。受测者在运动跑道上跑步，直到他已经到达力量极限，意味着他无法再增加速度了。In addition, the general non-invasive method of measuring lactic acid is the Conconi test (Conconi test) that is gradually becoming popular in sports medicine. In this method, the subject runs at a predetermined speed for the first 200 meters on a 400-meter sports track, and after 200 meters, the subject increases the pace in stages, for example, 0.5km/h respectively. At each 200-meter mark on the sports track, the subject noticed his heart rate at that time after circling the sports track respectively, and called out the data to the assistant. The subject ran on the exercise track until he had reached the limit of his strength, meaning he could no longer increase his speed.
对于测试的估计，将心跳率对着相关的跑速以二维(X-Y)作图。藉此发现结果：在有氧范围，给出较低的力量，心跳率几乎与跑速呈线性关系。此意味着心跳率随着受测者产生的力量等比例增加。此规律在转换至无氧代谢的阈值处被打破。在高出力的无氧范围，心跳率随着进一步增加的力量或跑速只稍微增加。心跳率相关于跑速的关系因而显示自低出力的有氧范围过渡至高出力的无氧范围的清楚、尖锐的骤变点，藉此决定乳酸阈值。对于乳酸阈值及相关跑速的心跳率特征可简单自X-Y图读取。For test estimation, heart rate is plotted in two dimensions (X-Y) against relative running speed. Here's what we found: In the aerobic range, given lower forces, there is an almost linear relationship between heart rate and running speed. This means that the heart rate increases in proportion to the force the subject generates. This rule is broken at the threshold of switching to anaerobic metabolism. In the high-effort anaerobic range, heart rate increases only slightly with further increases in force or running speed. The relationship of heart rate to running speed thus shows a clear, sharp transition from the low-effort aerobic range to the high-effort anaerobic range, thereby determining lactate threshold. Heart rate characteristics for lactate threshold and related running speed can be easily read from the X-Y diagram.
然而，康科尼测试比较繁复，且未有助手的情况下几乎无法执行。又，由于天候及受测者控制他的跑速的能力影响，康科尼测试量测的乳酸阈值比较不准确。However, the Conconi test is complex and almost impossible to perform without an assistant. Also, the Conconi test is less accurate in measuring lactate threshold due to weather and the subject's ability to control his running speed.
综上所述，需要一种低侵入性可用于连续经皮监测组织中的乳酸量测装置，提供给运动，健身，和国防工业之用，特别是运动员与运动爱好人士，使其保持肌肉不酸痛，并调整其运动强度，达到有效不伤害的健身目标。In summary, there is a need for a low-invasive device for continuous percutaneous monitoring of lactic acid in tissues, which can be used in sports, fitness, and defense industries, especially for athletes and sports enthusiasts, to keep muscles healthy. Soreness, and adjust its exercise intensity to achieve effective and non-injury fitness goals.
发明内容Contents of the invention
本发明的一目的，在于提供一种运动训练调整的方法，藉由具有微针的经皮感测器进行皮肤穿刺，低侵入性的穿刺能够有效减轻使用者的疼痛感，又同时达到取样组织液以量测人体中乳酸浓度值的目的。根据乳酸浓度值与一基准预设值的比较，帮助运动员或爱好运动人士调整运动强度及频率，进而达到最有效果的运动训练。One object of the present invention is to provide a method for adjusting sports training, which uses a percutaneous sensor with a microneedle to puncture the skin. The low-invasive puncture can effectively reduce the pain of the user and at the same time achieve sampling of tissue fluid For the purpose of measuring the concentration of lactic acid in the human body. According to the comparison between the lactic acid concentration value and a base preset value, it helps athletes or sports lovers to adjust the exercise intensity and frequency, so as to achieve the most effective exercise training.
为了达成上述的目的，本发明提供一种运动训练调整的方法，藉由包含一连续经皮微针感测器的乳酸量测装置量测人体中乳酸浓度，此运动训练调整的方法包括以下步骤：使用连续经皮微针感测器量测使用者人体组织中的乳酸浓度值；比较乳酸浓度值与一基准预设值；及假如乳酸浓度值高于基准预设值则通知使用者降低运动强度，反之假如乳酸浓度值低于基准预设值则通知使用者增加运动强度，确保用户肌肉不酸痛，足以完成全程比赛。进一步根据GPS或计步器等估测已经完成的距离与尚待完成的距离，可以有效调整安全的乳酸浓度值以及其安全的浓度变化率，以提高运动员比赛的成绩。同样的，对于各种球赛或各种团队运动比赛，个人的体能状况，是否需要休息，还是可持续完成，乳酸浓度值与其浓度变化率都是可以作为有效参考的依据。In order to achieve the above object, the present invention provides a method for adjusting sports training, by measuring the concentration of lactic acid in the human body through a lactic acid measuring device comprising a continuous transdermal microneedle sensor, the method for adjusting sports training includes the following steps : Use a continuous transdermal microneedle sensor to measure the lactic acid concentration in the user's body tissue; compare the lactic acid concentration with a baseline preset value; and notify the user to reduce exercise if the lactic acid concentration is higher than the baseline preset value On the contrary, if the lactic acid concentration value is lower than the baseline preset value, the user will be notified to increase the exercise intensity to ensure that the user's muscles are not sore, enough to complete the whole game. By further estimating the distance completed and the distance to be completed based on GPS or pedometer, the safe lactic acid concentration value and the safe concentration change rate can be effectively adjusted to improve the performance of athletes in competitions. Similarly, for various ball games or various team sports competitions, the individual's physical condition, whether it needs to rest or continue to complete, the lactic acid concentration value and its concentration change rate can be used as an effective reference basis.
本发明的另一目的，在于提供一种乳酸量测装置，藉由具有微针的经皮感测器进行皮肤穿刺，低侵入性的穿刺能够有效减轻使用者的疼痛感，又同时达到取样组织液以量测人体中乳酸浓度值的目的。Another object of the present invention is to provide a lactic acid measurement device, which uses a percutaneous sensor with a microneedle to puncture the skin. The low-invasive puncture can effectively reduce the user's pain and at the same time achieve sampling of tissue fluid For the purpose of measuring the concentration of lactic acid in the human body.
为了达成上述的目的，本发明还提供为一种乳酸量测装置，乳酸量测装置包含一连续经皮微针感测器及一比较器，比较器用于比较来自连续经皮微针感测器的信号处理单元的乳酸量测值和一基准预设值。本发明的乳酸量测装置的运作是基于乳酸感测酵素生成电活性过氧化氢，进而在一个极化白金电极产生安培电流，依赖于内外膜实现选择性和组织生物相容性。本发明的乳酸量测装置可被完全校准，不受体内常见的干扰物(如抗坏血酸和对乙酰氨基酚)影响。具有线性范围宽，覆盖了整个生理范围(可达25mM)，与对乳酸的高度选择性。In order to achieve the above object, the present invention also provides a lactic acid measurement device, the lactic acid measurement device includes a continuous transdermal microneedle sensor and a comparator, the comparator is used to compare the The lactic acid measurement value of the signal processing unit and a reference preset value. The operation of the lactic acid measuring device of the present invention is based on the lactic acid sensing enzyme generating electroactive hydrogen peroxide, and then generating an amperometric current at a polarized platinum electrode, relying on inner and outer membranes for selectivity and tissue biocompatibility. The lactate measuring device of the present invention can be fully calibrated and is not affected by common interfering substances in the body such as ascorbic acid and acetaminophen. It has a wide linear range, covering the entire physiological range (up to 25mM), and a high selectivity for lactic acid.
血乳酸堆积起始值(the onset of blood lactate accumulation,OBLA)是对应于4mM乳酸浓度的运动强度，它代表稳定状态下乳酸浓度的最大工作负荷，并对应于从容许负荷过渡到更严重的运动强度。不同的研究表明OBLA在长跑性能的重要性，它也被认为是训练引起的适应的一个敏感指标，以及精英和优秀的运动员之间的敏感鉴别指标。The onset of blood lactate accumulation (OBLA) is the exercise intensity corresponding to a lactate concentration of 4 mM, which represents the maximum work load of lactate concentration at steady state and corresponds to the transition from permissible load to more severe exercise strength. Different studies have shown the importance of OBLA in long-distance running performance, and it has also been recognized as a sensitive indicator of training-induced adaptation, as well as a sensitive discriminator between elite and elite athletes.
本发明的运动训练或比赛调整的方法，调整运动强度的策略基本上是以乳酸阈值(lactate threshold,LT)或无氧阈值,最大乳酸稳态值(maximal lactate steady state,MLSS),或是血乳酸堆积起始值(the onset of blood lactate accumulation,OBLA)作为基准预设值，当乳酸量测值超过这个基准预设值，并且乳酸变化值增加，则运动强度即可降低。以观察乳酸变化值是否减少，直到乳酸量测值回到基准预设值。若是超过基准预设值，但仍维持运动强度，并且乳酸变化值接近零，则表示，运动员今天的体能状况良好，可以维持此时的运动强度。更进一步，运动员可稍增加运动强度，并观察乳酸量测值是否持续增加，还是增加到一平衡点，即维持不变。经过如此有效的训练，运动员在相同的乳酸预设值，可以突破以往的运动强度，例如跑步，则其速度可加快；例如游泳，则其游泳速度可加快，因此比赛成绩都可进步。In the method for sports training or competition adjustment of the present invention, the strategy for adjusting exercise intensity is basically based on lactate threshold (lactate threshold, LT) or anaerobic threshold, maximum lactate steady state value (maximal lactate steady state, MLSS), or blood The onset of blood lactate accumulation (OBLA) is used as the baseline preset value. When the lactic acid measurement value exceeds the baseline preset value and the lactic acid change value increases, the exercise intensity can be reduced. To observe whether the lactic acid change value decreases until the lactic acid measurement value returns to the baseline preset value. If it exceeds the baseline preset value, but still maintains the exercise intensity, and the lactic acid change value is close to zero, it means that the athlete is in good physical condition today and can maintain the exercise intensity at this time. Furthermore, athletes can slightly increase the exercise intensity, and observe whether the lactic acid measurement value continues to increase, or increases to a balance point, that is, remains unchanged. After such effective training, athletes can break through the previous exercise intensity at the same lactic acid preset value, such as running, their speed can be accelerated; such as swimming, their swimming speed can be accelerated, so the competition performance can be improved.
另一个实施例，是以个人无氧阈值(individual anaerobic threshold,IAT)为一预设基准值，IAT被定义为在乳酸浓度比乳酸阈值(lactate threshold,LT)净增长1.5mM的时的跑步速度。In another embodiment, the personal anaerobic threshold (individual anaerobic threshold, IAT) is used as a preset reference value, and IAT is defined as the running speed when the lactic acid concentration is 1.5mM higher than the lactate threshold (lactate threshold, LT) net increase .
根据本发明的连续经皮微针感测器，此微针感测器包含：基板、微针单元、信号处理单元及电源单元。微针单元至少包含排列于基板上作为工作电极的第一微针组，以及作为参考电极的第二微针组，每一微针组至少包含一微针，第一微针组包含至少一薄片，每一薄片上至少设置一穿孔，穿孔边缘设置有一突刺，其中一薄片上的穿孔是供其余的薄片上相对位置的穿孔边缘的突刺穿过，且该些突刺互相分离。According to the continuous transdermal microneedle sensor of the present invention, the microneedle sensor includes: a substrate, a microneedle unit, a signal processing unit and a power supply unit. The microneedle unit at least includes a first microneedle group arranged on the substrate as a working electrode, and a second microneedle group as a reference electrode, each microneedle group includes at least one microneedle, and the first microneedle group includes at least one sheet Each sheet is provided with at least one perforation, and the edge of the perforation is provided with a thorn, wherein the perforation on one sheet is for the thorns on the edge of the perforation at the opposite position on the remaining sheets to pass through, and these thorns are separated from each other.
相较于现有技术，本发明使用一连续经皮微针感测器量测使用者人体组织中的乳酸浓度值，低侵入性的穿刺可降低感染的风险。而且，本发明的方法所使用的微针感测器可简便穿戴、准确量测人体组织中的乳酸浓度值，有利于使用者更准确地获知自己的生理参数，帮助调整运动强度及频率，进而达到最有效果的运动训练。Compared with the prior art, the present invention uses a continuous percutaneous microneedle sensor to measure the lactic acid concentration in the user's human tissue, and the low-invasive puncture can reduce the risk of infection. Moreover, the microneedle sensor used in the method of the present invention can be easily worn and accurately measure the concentration of lactic acid in human tissue, which is beneficial for users to know their own physiological parameters more accurately, help adjust exercise intensity and frequency, and further To achieve the most effective sports training.
以下结合附图和具体实施例对本发明进行详细描述，但不作为对本发明的限定。The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but not as a limitation of the present invention.
附图说明Description of drawings
图1本发明的一实施例运动训练调整的方法的步骤流程图。FIG. 1 is a flow chart of steps of a method for adjusting sports training according to an embodiment of the present invention.
图2本发明的一实施例连续经皮微针感测器的爆炸分解图；Fig. 2 is an exploded exploded view of a continuous percutaneous microneedle sensor according to an embodiment of the present invention;
图3与图2不同观看方向的本发明的一实施例连续经皮微针感测器的爆炸分解图；Fig. 3 is an exploded exploded view of a continuous percutaneous microneedle sensor according to an embodiment of the present invention viewed in different directions from Fig. 2;
图4本发明的一实施例微针单元的示意图；Fig. 4 is a schematic diagram of a microneedle unit according to an embodiment of the present invention;
图5本发明的一实施例工作电极微针组的结构局部俯视图；Fig. 5 is a partial top view of the structure of the working electrode microneedle group according to an embodiment of the present invention;
图6本发明的另一实施例工作电极微针组的结构局部俯视图；Fig. 6 is a partial top view of the structure of the working electrode microneedle group according to another embodiment of the present invention;
图7本发明的又一实施例工作电极微针组的结构局部俯视图；Fig. 7 is a partial top view of the structure of the working electrode microneedle group in another embodiment of the present invention;
图8本发明的又另一实施例工作电极微针组的结构局部俯视图；Fig. 8 is a partial top view of the structure of the working electrode microneedle group in yet another embodiment of the present invention;
图9本发明的一实施例连续经皮微针感测器的组合外观示意图；Fig. 9 is a schematic diagram of the combined appearance of the continuous transdermal microneedle sensor according to an embodiment of the present invention;
图10本发明的一实施例连续经皮微针感测器的组合剖视示意图；Fig. 10 is a combined cross-sectional schematic diagram of a continuous percutaneous microneedle sensor according to an embodiment of the present invention;
图11为图11的局部剖视示意图，其中乳酸感测酵素是涂布在微针的突刺上；Fig. 11 is a partial cross-sectional schematic diagram of Fig. 11, wherein the lactic acid sensing enzyme is coated on the thorn of the microneedle;
图12为图11中突刺的局部放大示意图；Fig. 12 is a partially enlarged schematic diagram of the spur in Fig. 11;
图13为图10的局部剖视示意图，其中乳酸感测酵素是涂布在试纸片上；及Figure 13 is a partial cross-sectional schematic diagram of Figure 10, wherein the lactic acid sensing enzyme is coated on the test paper sheet; and
图14为图13中试片纸的局部放大示意图。Fig. 14 is a partially enlarged schematic view of the test piece in Fig. 13 .
具体实施方式Detailed ways
有关本发明的详细说明及技术内容，配合附图说明如下，然而所附的附图仅提供参考与说明用，并非用来对本发明加以限制。The detailed description and technical content of the present invention are described below with accompanying drawings. However, the attached drawings are provided for reference and illustration only, and are not intended to limit the present invention.
请参照图1，图1本发明的一实施例运动训练调整的方法，藉由包含一连续经皮微针感测器的乳酸量测装置量测人体中乳酸浓度，此运动训练调整的方法，包括以下步骤：步骤S10，使用一连续经皮微针感测器量测使用者人体组织中的乳酸浓度值；步骤S12，比较乳酸浓度值与一基准预设值；及步骤S14，假如乳酸浓度值高于基准预设值则通知使用者降低运动强度，反之假如乳酸浓度值低于基准预设值则通知使用者增加运动强度。步骤S12中的基准预设值是参考一般人的基本生理数据，例如年龄、性别、身高及体重等，获得的乳酸浓度值。Please refer to Fig. 1, Fig. 1 is a method for adjusting sports training according to an embodiment of the present invention. The lactic acid concentration in the human body is measured by a lactic acid measuring device including a continuous transdermal microneedle sensor. This method for adjusting sports training, It includes the following steps: Step S10, using a continuous transdermal microneedle sensor to measure the lactic acid concentration value in the user's human tissue; Step S12, comparing the lactic acid concentration value with a reference preset value; and Step S14, if the lactic acid concentration value If the value is higher than the base preset value, the user is notified to reduce the exercise intensity; otherwise, if the lactic acid concentration value is lower than the base preset value, the user is notified to increase the exercise intensity. The reference preset value in step S12 is the lactic acid concentration value obtained by referring to the basic physiological data of ordinary people, such as age, gender, height and weight.
本发明的一实施例中，乳酸量测装置包含一连续经皮微针感测器及一比较器(图未示)，比较器是用于比较来自该信号处理单元的乳酸量测值和一基准预设值。In one embodiment of the present invention, the lactic acid measurement device includes a continuous transdermal microneedle sensor and a comparator (not shown), the comparator is used to compare the lactic acid measurement value from the signal processing unit with a Baseline presets.
请参照图2和图3，图2和图3分别由不同方向观看本发明的一实施例连续经皮微针感测器的爆炸分解图。本发明的连续经皮微针感测器包含：基板10、微针单元20、可挠性垫片30、信号处理单元41、电源单元43及外盖50，其中信号处理单元41和电源单元43是设置于电路板40上。Please refer to FIG. 2 and FIG. 3 . FIG. 2 and FIG. 3 are exploded views of a continuous transdermal microneedle sensor according to an embodiment of the present invention viewed from different directions. The continuous percutaneous microneedle sensor of the present invention comprises: a substrate 10, a microneedle unit 20, a flexible spacer 30, a signal processing unit 41, a power supply unit 43 and an outer cover 50, wherein the signal processing unit 41 and the power supply unit 43 is set on the circuit board 40 .
根据本发明的一实施例，微针单元20包含排列于基板10上覆盖磺化聚醚醚砜/聚醚砜膜和乳酸感测酵素作为工作电极的第一微针组22、覆盖氧化铱或其他生物相容的导电材料作为参考电极的第二微针组24，以及覆盖白金作为反电极的第三微针组26。第一微针组22的该些微针可以例如是阵列形式排列于基板10上。可挠性垫片30上具有一开口32供微针单元20通过，且微针单元20以导电柱21、23、25与电路板40上的电接点42、44、46电性连接。由于本发明具有可挠性垫片30，操作时可与使用者的肌肉轮廓共型，紧密接触。当微针组插入皮下的组织，并于电极间施加极化电压，乳酸感测酵素与组织液内的乳酸反应生成电活性过氧化氢(H2O2)，进而在工作电极产生安培电流，该电流与乳酸浓度成比例关系。According to an embodiment of the present invention, the microneedle unit 20 includes a first microneedle group 22 arranged on the substrate 10 covered with a sulfonated polyether ether sulfone/polyether sulfone membrane and a lactic acid sensing enzyme as a working electrode, covered with iridium oxide or Another biocompatible conductive material is used as the second microneedle set 24 as the reference electrode, and the third microneedle set 26 covered with platinum is used as the counter electrode. The microneedles of the first microneedle group 22 can be arranged on the substrate 10 in the form of an array, for example. The flexible gasket 30 has an opening 32 for the microneedle unit 20 to pass through, and the microneedle unit 20 is electrically connected to the electrical contacts 42 , 44 , 46 on the circuit board 40 through the conductive posts 21 , 23 , 25 . Since the present invention has the flexible spacer 30, it can conform to the contour of the user's muscles and be in close contact with it during operation. When the microneedle group is inserted into the subcutaneous tissue and a polarizing voltage is applied between the electrodes, the lactic acid sensing enzyme reacts with the lactic acid in the interstitial fluid to generate electroactive hydrogen peroxide (H2O2), which in turn generates an ampere current at the working electrode, which is compatible with the lactic acid Concentrations are proportional.
信号处理单元41与微针单元20电性连接以接收微针感测的目标分子浓度，经运算判定后，将信息转换成一感测信号，也是一种能够反映使用者当下的生理状态的信号。电源单元43是供应工作电力至本发明的连续经皮微针感测器。The signal processing unit 41 is electrically connected to the microneedle unit 20 to receive the concentration of the target molecule sensed by the microneedle, and after calculation and determination, convert the information into a sensing signal, which is also a signal that can reflect the user's current physiological state. The power supply unit 43 supplies operating power to the continuous transdermal microneedle sensor of the present invention.
请参照图4，图4本发明的一实施例微针单元的示意图。第一微针组22系由第一薄片222和第二薄片224叠置而成，第一薄片222上至少设置一第一穿孔2222，该第一穿孔2222边缘设置有一第一突刺2224，及第二薄片224上至少设置一第二穿孔2242，第二穿孔边缘设置有一第二突刺2244，第二突刺2244穿过第一薄片222上相对位置的第一穿孔2222与第一突刺2224相对。此外，第一微针组22的第二薄片224边缘上可设置倒钩2246与基板10上的孔穴102卡合。在一实施例，第一微针组22的第二薄片224边缘上可设置导柄2248插入基板10上的插槽104，藉由电路与导电柱21电性连接。Please refer to FIG. 4 , which is a schematic diagram of a microneedle unit according to an embodiment of the present invention. The first microneedle group 22 is formed by stacking a first sheet 222 and a second sheet 224. The first sheet 222 is provided with at least a first perforation 2222, and the edge of the first perforation 2222 is provided with a first thorn 2224, and a second At least one second perforation 2242 is disposed on the second sheet 224 , and a second protrusion 2244 is disposed on the edge of the second perforation. In addition, barbs 2246 can be provided on the edge of the second sheet 224 of the first microneedle group 22 to engage with the holes 102 on the substrate 10 . In one embodiment, a guide handle 2248 can be provided on the edge of the second sheet 224 of the first microneedle set 22 to be inserted into the slot 104 on the substrate 10 , and electrically connected to the conductive column 21 through a circuit.
同理，第二微针组24也具有第一薄片242，第一薄片242上至少设置一第一穿孔2422，第一穿孔边缘设置有一第一突刺2424。此外，第二微针组24的第一薄片242边缘上可设置倒钩2426与基板10上的孔穴102卡合。在一实施例，第二微针组24的第一薄片242边缘上可设置导柄2428插入基板10上的插槽104，藉由电路与导电柱23电性连接。Similarly, the second microneedle group 24 also has a first sheet 242 , at least one first perforation 2422 is provided on the first sheet 242 , and a first protrusion 2424 is provided on the edge of the first perforation. In addition, barbs 2426 can be provided on the edge of the first sheet 242 of the second microneedle group 24 to engage with the holes 102 on the substrate 10 . In one embodiment, a guide handle 2428 can be provided on the edge of the first sheet 242 of the second microneedle group 24 to be inserted into the slot 104 on the substrate 10 , and electrically connected to the conductive column 23 through a circuit.
同理，第三微针组26也具有第一薄片262，第一薄片262上至少设置一第一穿孔2622，第一穿孔2622边缘设置有一第一突刺2624。此外，第三微针组26的第一薄片262边缘上可设置倒钩2626与基板10上的孔穴102卡合。在一实施例，第三微针组26的第一薄片262边缘上可设置导柄2628插入基板10上的插槽104，藉由电路与导电柱25电性连接。Similarly, the third microneedle group 26 also has a first sheet 262 , at least one first perforation 2622 is provided on the first sheet 262 , and a first protrusion 2624 is provided on the edge of the first perforation 2622 . In addition, barbs 2626 can be provided on the edge of the first sheet 262 of the third microneedle group 26 to engage with the holes 102 on the substrate 10 . In one embodiment, a guide handle 2628 can be provided on the edge of the first sheet 262 of the third microneedle set 26 to be inserted into the slot 104 on the substrate 10 , and electrically connected to the conductive column 25 through a circuit.
本发明的一实施例，第一微针组22、第二微针组24和第三微针组26的微针是藉由冲压或蚀刻工艺形成。该些突刺的材料是选自不锈钢、镍、镍合金、钛、钛合金、纳米碳管或硅材料。该些突刺的材料也可以是树脂例如是聚碳酸酯、聚甲基丙烯酸共聚物、乙烯/醋酸乙烯酯共聚物、铁氟龙或聚酯类，并于表面沉积具有生物相容性的金属。该些突刺的高度为300-600微米、基底宽度为150-450微米。该些突刺的尖端部的间隔为500-3000微米。In one embodiment of the present invention, the microneedles of the first microneedle group 22 , the second microneedle group 24 and the third microneedle group 26 are formed by stamping or etching process. The materials of the protrusions are selected from stainless steel, nickel, nickel alloy, titanium, titanium alloy, carbon nanotube or silicon material. The material of these spikes can also be resin such as polycarbonate, polymethacrylic acid copolymer, ethylene/vinyl acetate copolymer, Teflon or polyester, and a biocompatible metal is deposited on the surface. The spikes have a height of 300-600 microns and a base width of 150-450 microns. The distance between the tips of the spikes is 500-3000 microns.
请参考图5至图8。图5本发明的一实施例工作电极微针组的结构局部俯视图。第一微针组22是由第一薄片222和第二薄片224叠置而成，第一薄片222上至少设置一第一穿孔2222，第一穿孔2222边缘设置有一第一突刺2224，及第二薄片224上至少设置一第二穿孔2242，第二穿孔边缘设置有一第二突刺2244，第二突刺2244穿过第一薄片222上相对位置的第一穿孔2222与第一突刺2224相对。Please refer to Figure 5 to Figure 8. Fig. 5 is a partial top view of the structure of the working electrode microneedle group according to an embodiment of the present invention. The first microneedle group 22 is formed by stacking a first sheet 222 and a second sheet 224. At least one first perforation 2222 is provided on the first sheet 222, and a first thorn 2224 is provided on the edge of the first perforation 2222, and a second At least one second perforation 2242 is disposed on the sheet 224 , and a second protrusion 2244 is disposed on the edge of the second perforation.
图6本发明的另一实施例工作电极微针组的结构局部俯视图。第一微针组22是由第一薄片222、第二薄片224和第三薄片226叠置而成，第一薄片222上至少设置第一穿孔2222，第一穿孔2222边缘设置有一第一突刺2224，第二薄片224上至少设置一第二穿孔2242，第二穿孔2242边缘设置有一第二突刺2244，及第三薄片226上至少设置一第三穿孔2262，第三穿孔2262边缘设置有一第三突刺2264，第二突刺2244和第三突刺2264穿过第一薄片222上的第一穿孔2222与第一突刺2224呈正三角锥形。Fig. 6 is a partial top view of the structure of the working electrode microneedle group according to another embodiment of the present invention. The first microneedle group 22 is formed by stacking a first sheet 222, a second sheet 224, and a third sheet 226. The first sheet 222 is provided with at least a first perforation 2222, and the edge of the first perforation 2222 is provided with a first thorn 2224. , at least one second perforation 2242 is set on the second sheet 224, and a second puncture 2244 is provided on the edge of the second perforation 2242, and at least one third perforation 2262 is provided on the third sheet 226, and a third thorn is provided on the edge of the third perforation 2262 2264 , the second thorn 2244 and the third thorn 2264 pass through the first perforation 2222 on the first sheet 222 and the first thorn 2224 is in the shape of an equilateral triangle.
图7本发明的又一实施例工作电极微针组的结构局部俯视图。第一微针组22是由第一薄片222、第二薄片224、第三薄片226叠置而成，其中第一薄片222上至少设置一第一穿孔2222，第一穿孔2222边缘设置有一第一突刺2224；第二薄片224上至少设置一第二穿孔2242，第二穿孔2242边缘设置有一第二突刺2244；及第三薄片226上至少设置一第三穿孔2262，第三穿孔2262边缘设置有一第三突刺2264，将第二突刺2244和第三突刺2264穿过第一薄片222上的第一穿孔2222与第一突刺2224相邻排列，呈等腰直角三角锥形。Fig. 7 is a partial top view of the structure of the working electrode microneedle group according to another embodiment of the present invention. The first microneedle group 22 is formed by stacking a first sheet 222, a second sheet 224, and a third sheet 226, wherein the first sheet 222 is provided with at least a first perforation 2222, and the edge of the first perforation 2222 is provided with a first Thrust 2224; at least one second perforation 2242 is set on the second sheet 224, and a second perforation 2244 is provided on the edge of the second perforation 2242; Three protrusions 2264, the second protrusions 2244 and the third protrusions 2264 pass through the first perforation 2222 on the first sheet 222 and are arranged adjacent to the first protrusions 2224, forming an isosceles right triangle pyramid.
图8本发明的又另一实施例工作电极微针组的结构局部俯视图。第一微针组22是由第一薄片222、第二薄片224、第三薄片226和第四薄片228叠置而成，第一薄片222上至少设置一第一穿孔2222，第一穿孔2222边缘设置有一第一突刺2224，第二薄片224上至少设置一第二穿孔2242，第二穿孔2242边缘设置有一第二突刺2244，第三薄片226上至少设置一第三穿孔2262，第三穿孔2262边缘设置有一第三突刺2264及第四薄片228上至少设置一第四穿孔2282，第四穿孔2282边缘设置有一第四突刺2284，第二突刺2244、第三突刺2264和第四突刺2284穿过第一薄片222上的第一穿孔2222与第一突刺2224呈四角锥形。Fig. 8 is a partial top view of the structure of the working electrode microneedle group according to yet another embodiment of the present invention. The first microneedle group 22 is formed by overlapping the first sheet 222, the second sheet 224, the third sheet 226 and the fourth sheet 228. At least one first perforation 2222 is set on the first sheet 222, and the edge of the first perforation 2222 A first thorn 2224 is provided, at least a second perforation 2242 is provided on the second sheet 224, a second puncture 2244 is provided on the edge of the second perforation 2242, a third perforation 2262 is at least provided on the third sheet 226, and a third perforation 2262 is provided on the edge A third thorn 2264 and at least a fourth perforation 2282 are arranged on the fourth sheet 228, a fourth thorn 2284 is arranged on the edge of the fourth perforation 2282, and the second thorn 2244, the third thorn 2264 and the fourth thorn 2284 pass through the first The first perforation 2222 and the first protrusion 2224 on the sheet 222 are in the shape of a quadrangular pyramid.
图5至图8所示的四个实施例中，第一微针组22的每一突刺2224包含一尖端部2221及一基底2223，其中一薄片上的穿孔经其余的薄片上相对位置的穿孔边缘的突刺穿过后形成的该微针的该些尖端部的顶部不在同一高度。或者，可以依照该些薄片重叠的次序，预先设计其突刺的高度，使其中一薄片上的穿孔经其余的薄片上相对位置的穿孔边缘的突刺穿过后形成的该微针的该些尖端部的顶部具有同一高度。In the four embodiments shown in Fig. 5 to Fig. 8, each thrust 2224 of the first microneedle group 22 comprises a tip portion 2221 and a base 2223, wherein the perforation on one sheet is passed through the perforation at the opposite position on the remaining sheet The tops of the tips of the microneedles formed after the spurs of the edges pass through are not at the same height. Or, the height of the thorns can be pre-designed according to the overlapping order of the sheets, so that the perforations on one of the sheets pass through the piercing edges of the corresponding perforations on the remaining sheets to form the tips of the microneedles. The tops have the same height.
接着，请参考图9和图10。图9本发明的一实施例连续经皮微针感测器的组合外观示意图。图10本发明的一实施例连续经皮微针感测器的组合剖视示意图。本实施例中的第一微针组22是由第一薄片222和第二薄片224叠置而成，可例如施加一冲压力于第一薄片222和第二薄片224的四周以结合两者。第二微针组24只具有第一薄片242。第三微针组26也只具有第一薄片262。由于本发明具有可挠性垫片30，操作时可与使用者的肌肉轮廓共型，紧密接触。Next, please refer to FIG. 9 and FIG. 10 . FIG. 9 is a schematic diagram of the assembled appearance of the continuous transdermal microneedle sensor according to an embodiment of the present invention. FIG. 10 is a schematic cross-sectional view of a continuous transdermal microneedle sensor according to an embodiment of the present invention. The first microneedle set 22 in this embodiment is formed by stacking the first sheet 222 and the second sheet 224 , for example, a stamping force can be applied to the surroundings of the first sheet 222 and the second sheet 224 to combine them. The second microneedle group 24 only has the first sheet 242 . The third microneedle group 26 also only has the first sheet 262 . Since the present invention has the flexible spacer 30, it can conform to the contour of the user's muscles and be in close contact with it during operation.
接着，请参考图11，图11为图10的局部剖视示意图，其中乳酸感测酵素是涂布在微针的突刺上以形成一乳酸酵素层。具体而言，乳酸感测酵素是涂布在突刺的内表面上，突刺的外表面上涂布有抗皮肤过敏的药物。一个表面涂有乳酸感测酵素的微针的连续经皮微针感测器，可用以检测皮肤表层中的乳酸浓度，且此浓度可作为判定生理状态的指标之一。Next, please refer to FIG. 11 , which is a partial cross-sectional schematic diagram of FIG. 10 , wherein the lactic acid sensing enzyme is coated on the puncture of the microneedle to form a lactic acid enzyme layer. Specifically, the lactic acid sensing enzyme is coated on the inner surface of the spine, and the anti-skin allergy medicine is coated on the outer surface of the spine. A continuous transdermal microneedle sensor with microneedles coated with lactic acid sensing enzymes can be used to detect the concentration of lactic acid in the surface layer of the skin, and this concentration can be used as one of the indicators to determine the physiological state.
请参考图12，图12为图11中突刺的局部放大示意图。由于安培计电化学方法一般比较不具选择性，许多常见的干扰物存在于血浆中，最显著为抗坏血酸(ascorbic acid)，会导入信号之中。为了实现高的乳酸选择性，于电极的表面上先形成一半透膜941例如磺化聚醚醚砜/聚醚砜(SPEES/PES)膜，再于半透膜941上形成乳酸酵素层943。带负电荷的SPEES/PES高度不透抗坏血酸，同时又容易让非常小的中性分子如过氧化氢渗透。又，为了避免乳酸感测酵素或抗皮肤过敏的药物受到环境污染，可于乳酸酵素层943或抗皮肤过敏的药物的表面上形成一保护层945，保护层945例如是环氧树脂-聚胺酯甲酸基树脂(Epoxy-PU)膜。外层的环氧树脂-聚胺酯甲酸基树脂膜具有多种功能：(i)保护乳酸酵素层免受测量环境的影响，(ⅱ)防止乳酸感测酵素的浸出，和(iii)提供乳酸量测装置和组织之间生物相容的界面。此外，环氧树脂-聚胺酯甲酸基树脂膜同时限制乳酸扩散至感测表面，但允许让氧分子自由扩散有助于酶促反应，从而使线性范围加宽成为可能，以免被酶动力学反应限制。Please refer to FIG. 12 . FIG. 12 is a partially enlarged schematic view of the thorn in FIG. 11 . Since amperometric electrochemical methods are generally less selective, many common interferents present in plasma, most notably ascorbic acid, are introduced into the signal. In order to achieve high lactic acid selectivity, a semipermeable membrane 941 such as a sulfonated polyetherethersulfone/polyethersulfone (SPEES/PES) membrane is first formed on the surface of the electrode, and then a lactic acid enzyme layer 943 is formed on the semipermeable membrane 941 . Negatively charged SPEES/PES are highly impermeable to ascorbic acid while being readily permeable to very small neutral molecules such as hydrogen peroxide. Also, in order to avoid the lactic acid sensing enzyme or the anti-skin allergy medicine from being polluted by the environment, a protective layer 945 can be formed on the surface of the lactic acid ferment layer 943 or the anti-skin allergy medicine. The protective layer 945 is, for example, epoxy resin-polyurethane formic acid Base resin (Epoxy-PU) film. The outer epoxy-polyurethane-based resin film serves multiple functions: (i) protects the lactic acid enzyme layer from the measurement environment, (ii) prevents leaching of the lactic acid sensing enzyme, and (iii) provides lactic acid measurement Biocompatible interface between device and tissue. In addition, the epoxy-polyurethane-based resin film simultaneously limits the diffusion of lactic acid to the sensing surface, but allows the free diffusion of oxygen molecules to facilitate the enzymatic reaction, thereby enabling a broadened linear range so as not to be limited by the kinetic reaction of the enzyme .
图13为图10的局部剖视示意图，其中乳酸感测酵素是涂布在试纸片上。本实施例与图10所示实施例的差异在于，本实施例的第一微针组22是作为萃取间质液的工具，突刺上并不涂布乳酸感测酵素，乳酸感测酵素是涂布在位于第一微针组22下方的试纸片的表面上。本实施例中，试纸片是安置于第一微针组22与基板10之间，试纸片包含一导电层92及位于导电层92上的多个测试区域94，该些测试区域94上是涂布乳酸感测酵素以形成一乳酸酵素层，且与第一微针组22上的穿孔2222对齐。本实施例是使用树脂片96于其上定义出该些测试区域94。此外，第一微针组22是藉由一黏着层98与试纸片接合。FIG. 13 is a partial cross-sectional schematic diagram of FIG. 10 , wherein the lactate-sensing enzyme is coated on a test paper sheet. The difference between this embodiment and the embodiment shown in FIG. 10 is that the first microneedle group 22 of this embodiment is used as a tool for extracting interstitial fluid, and the punctures are not coated with lactic acid sensing enzyme, and the lactic acid sensing enzyme is coated The cloth is placed on the surface of the test paper sheet located below the first microneedle group 22 . In this embodiment, the test paper sheet is arranged between the first microneedle group 22 and the substrate 10. The test paper sheet includes a conductive layer 92 and a plurality of test areas 94 located on the conductive layer 92. These test areas 94 are coated with The lactic acid sensing enzyme is laid to form a lactic acid enzyme layer, which is aligned with the perforation 2222 on the first microneedle set 22 . In this embodiment, a resin sheet 96 is used to define the testing areas 94 thereon. In addition, the first microneedle set 22 is bonded to the test paper sheet through an adhesive layer 98 .
接着，请参考图14，图14为图13中试片纸的局部放大示意图。同理，为了实现高的乳酸选择性，于电极的表面上先形成一半透膜941例如磺化聚醚醚砜/聚醚砜(SPEES/PES)膜，再于半透膜941上形成乳酸酵素层943。SPEES/PES高度不透抗坏血酸，同时又容易让非常小的中性分子如过氧化氢渗透。又，为了避免乳酸感测酵素或抗皮肤过敏的药物受到环境污染，可于乳酸酵素层943或抗皮肤过敏的药物的表面上形成一保护层945，保护层945例如是环氧树脂-聚胺酯甲酸基树脂(Epoxy-PU)膜。乳酸感测酵素除了乳酸氧化酶之外，也可选自乳酸脱氢酶(lactate dehydrogenase)、细胞色素b2(Cytochrome b2)、乳酸单加氧酶(lactate monooxygenase)、或过氧化氢酶(hydrogen peroxidase)等。Next, please refer to FIG. 14 , which is a partially enlarged schematic diagram of the test strip in FIG. 13 . Similarly, in order to achieve high lactic acid selectivity, a semipermeable membrane 941 such as a sulfonated polyetherethersulfone/polyethersulfone (SPEES/PES) membrane is first formed on the surface of the electrode, and then a lactic acid enzyme is formed on the semipermeable membrane 941. Layer 943. SPEES/PES is highly impermeable to ascorbic acid while being easily permeable to very small neutral molecules such as hydrogen peroxide. Also, in order to prevent the lactic acid sensing enzyme or the anti-skin allergy medicine from being polluted by the environment, a protective layer 945 can be formed on the surface of the lactic acid ferment layer 943 or the anti-skin allergy medicine. The protective layer 945 is, for example, epoxy resin-polyurethane formic acid Base resin (Epoxy-PU) film. In addition to lactate oxidase, the lactate sensing enzyme can also be selected from lactate dehydrogenase (lactate dehydrogenase), cytochrome b2 (Cytochrome b2), lactate monooxygenase (lactate monooxygenase), or catalase (hydrogen peroxidase) )Wait.
当然，本发明还可有其它多种实施例，在不背离本发明精神及其实质的情况下，熟悉本领域的技术人员当可根据本发明作出各种相应的改变和变形，但这些相应的改变和变形都应属于本发明所附的权利要求的保护范围。Certainly, the present invention also can have other multiple embodiments, without departing from the spirit and essence of the present invention, those skilled in the art can make various corresponding changes and deformations according to the present invention, but these corresponding Changes and deformations should belong to the scope of protection of the appended claims of the present invention.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410448944.5ACN105455855B (en) | 2014-09-04 | 2014-09-04 | Lactic acid measuring device and exercise training adjusting method |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410448944.5ACN105455855B (en) | 2014-09-04 | 2014-09-04 | Lactic acid measuring device and exercise training adjusting method |
| Publication Number | Publication Date |
|---|---|
| CN105455855A CN105455855A (en) | 2016-04-06 |
| CN105455855Btrue CN105455855B (en) | 2018-05-25 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410448944.5AActiveCN105455855B (en) | 2014-09-04 | 2014-09-04 | Lactic acid measuring device and exercise training adjusting method |
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