Lung tissue single channel fixerTechnical field
The present invention relates to a kind of tissue fixation device, especially a kind of lung tissue single channel fixer.
Background technique
When doing animal lung tissue's pathological section, need that lung tissue is fixed, when lung tissue is dyed and is observed, weIt not only needs to observe clearly stained tissue, more requires to keep the intact of pulmonary morphology, avoid disorganization, alveolar collapseThe problems such as.
Currently used lung tissue fixing means mainly has 3 kinds: 1, using embedded box, lung tissue being fixed on formalinIn.2, one small cotton is put in the formalin liquid level for being put into lung tissue, infiltrates lung tissue.3, with perfusion tube by tracheae toIntrapulmonary injects a small amount of formalin.
Although application method 1 can will cause serious lung in the cracking infiltration to formalin of lung tissueIt withers, destroys the normal organization of lung, and fixer belongs to nature submergence, speed is slower, is easy to influence in experimentExperimental result.The speed that 2 formalin of method immerses lung tissue is also slow, equally can not achieve the desired results.And method 3 is adoptedWith the mode of perfusion, hematocele when perfusion in lung tissue can be adhered to lung surface, influence chipping qualities and observing effect.
Summary of the invention
It is slow that the present invention solves existing lung tissue fixed speed, influences fixer submergence efficiency, causes lung tissue exposureOverlong time influences the defect of experimental result, provides a kind of lung tissue single channel fixer, air core pulling is arranged in single channel,Fixer inner air is extracted by air core pulling, accelerates the discharge of lung tissue inner air, while also accelerating fixer and immersing, contractingShort lung tissue exposure duration, it is ensured that experimental result is correct.
This invention also solves causing tissue seriously miniature when existing lung tissue is fixed, it is easily destroyed the normal of lung tissueStructure influences the defect of experimental result, provides a kind of lung tissue single channel fixer, and lung tissue is sunk naturally, taken out by airCore accelerates the discharge of lung tissue inner air, accelerates fixer and immerses, will not destroy lung tissue normal configuration, it is ensured that experimental result is justReally.
The technical solution adopted by the present invention to solve the technical problems is: a kind of lung tissue single channel fixer, including oneA fixed ontology, fixed body interior are provided with the accommodating chamber for containing fixer, are provided with a connection on fixed ontology and accommodateThe opening of chamber, opening are provided with sealing cover, the wing passage that this body sidewall is provided with a connection accommodating chamber are fixed, in wing passageIt is provided with air core pulling.Fixer, sealing cover closing opening are loaded in accommodating chamber, lung tissue is put into accommodating chamber from openingInterior, as air core pulling removes outward, the volume of accommodating chamber increases, so that the pressure inside accommodating chamber reduces to form negative pressure shapeState, under subnormal ambient, the air inside lung tissue is by rapid deflation, and under the elasticity of lung tissue itself, fixer soaks rapidlyEntering into alveolar, alveolar restores to the original state, and the position of those lesions loses original function, and morphosis remains unchanged, so thatLung tissue cell is fixed complete, convenient for doing immunohistochemistry and pathological analysis below.
Preferably, being provided with several positioning elements on fixed ontology, several and positioning element is provided in air core pullingThe fixation member matched, fixation member match the extraction length for limiting air core pulling with corresponding positioning element.
Preferably, air core pulling is in piston-like, including cylindrical piston head and piston rod, piston head are in wing passageInterior, piston rod reaches outside wing passage.
Preferably, piston head is in the form of annular discs, the periphery of disk is provided with three grooves, and groove has been embedded in sealing ring,In three seals circle, intermediate for O-ring seal, two sides are rectangular seal.
Preferably, the height of wing passage is equal with the height of accommodating chamber, i.e. air core pulling directly will fixed body interiorIt is separated into accommodating chamber and wing passage.
Preferably, the height of wing passage is lower than the height of accommodating chamber, wing passage is in the position of fixed body upper part, GuThe liquid level of liquid is determined lower than wing passage lower position.
Preferably, the surface of sealing cover towards accommodating chamber is connected with a hollow expander, inside expander filled withThe air of standard pressure.Accommodating chamber removes outward with air core pulling and gradually forms subnormal ambient, expander expansion, and expander hasHave suitable elasticity, the expansion of expander lags behind the emptying of lung tissue air, etc. when fixer immerses after the emptying of lung tissues air,Expander just expands, and the pressure inside accommodating chamber has slight rebound at this time, this, which gos up, just helps alveolar to restore to the original state.
The beneficial effects of the present invention are: be loaded with fixer in accommodating chamber, sealing cover closing opening, with air core pulling toIt removes outside, the volume of accommodating chamber increases, so that the pressure inside accommodating chamber reduces to form negative pressure state, lung tissue is put from openingEnter into accommodating chamber, under subnormal ambient, the air inside lung tissue is by rapid deflation, under the elasticity of lung tissue itself, GuDetermine liquid to be immersed in alveolar rapidly, alveolar restores to the original state, the position of those lesions, loses original function, and morphosis is protectedHold it is constant so that lung tissue cell is fixed complete, convenient for doing immunohistochemistry and pathological analysis below.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the present invention；
Fig. 2 is the use schematic diagram of structure shown in Fig. 1 of the present invention；
Fig. 3 is second of structural schematic diagram of the invention；
Fig. 4 is the use schematic diagram of structure shown in Fig. 3 of the present invention；
In figure: 1, fixed ontology, 2, wing passage, 3, air core pulling, 4, fixation member, 5, positioning element, 6, rectangular sealCircle, 7, O-ring seal, 8, fixer, 9, accommodating chamber, 10, sealing cover.
Specific embodiment
Below by specific embodiment, and in conjunction with attached drawing, the technical solutions of the present invention will be further described.
Embodiment 1: a kind of lung tissue single channel fixer (referring to Fig. 1), including a fixed ontology 1, fixed ontology are inCylinder, fixed body interior are provided with the accommodating chamber 9 for containing fixer 8, and fixed body upper part is provided with a connection and accommodatesThe opening of chamber, opening are provided with sealing cover 10, fix the wing passage 2 that this body sidewall is provided with a connection accommodating chamber, and side is logicalAir core pulling 3 is provided in road.
The height of wing passage and the height of accommodating chamber are equal, i.e. fixed body interior is directly separated into receiving by air core pullingChamber and wing passage.Air core pulling is in piston-like, including cylindrical piston head and piston rod, piston head are in wing passage, pistonBar reaches outside wing passage.Piston head is in the form of annular discs, and the periphery of disk is provided with three grooves, and groove has been embedded in sealing ring,In three seals circle, intermediate for O-ring seal 7, two sides are rectangular seal 6.Several positioning are provided on fixed ontologyComponent, is provided with several fixation members matched with positioning element in air core pulling, fixation member and corresponding positioning elementMatch the extraction length for limiting air core pulling.
Fixer, i.e. 10% formalin are first filled in accommodating chamber, the liquid level of fixer is in the 2/3 of accommodating chamberLung tissue is then put by eminence from opening, and horse back close encapsulation lid then removes air core pulling outward, accommodating chamberVolume increases, so that the pressure inside accommodating chamber reduces to form negative pressure state, the air quilt under subnormal ambient, inside lung tissueRapid deflation, under the elasticity of lung tissue itself, fixer is immersed in alveolar rapidly, and alveolar restores to the original state.Fixation member withCorresponding one of positioning element, which matches, fixes air core pulling, lasts long enough, until lung tissue is soaked completelyNot (referring to fig. 2).
Embodiment 2: a kind of lung tissue single channel fixer (referring to Fig. 3 Fig. 4), difference from Example 1 is: side is logicalThe height in road is lower than the height of accommodating chamber, and wing passage is in the position of fixed body upper part, and the liquid level of fixer is lower than wing passageLower position.Remaining structure is referring to embodiment 1.
Embodiment 3: a kind of lung tissue single channel fixer, place unlike the embodiments above are: sealing cover direction is heldReceiving the surface of chamber is connected with a hollow expander, the air filled with standard pressure inside expander.The expansion system of expanderNumber is suitable with the elastic restitution coefficient of lung tissue, after the expansion of expander betides the emptying of lung tissue air.Remaining structure referenceEmbodiment 1 or embodiment 2.
Embodiment described above is several preferred versions of the invention, not makees limit in any form to the present inventionSystem, there are also other variations and modifications on the premise of not exceeding the technical scheme recorded in the claims.