Modified version bio-reactorTechnical field
The present invention relates to a kind of modified version bio-reactor, belong to biological technical field.
Background technology
The charging line of bio-reactor is for supplementing nutrient solution, inoculating cell etc. in the tank body of bio-reactor.In macro-organism reactor, the volume of required cell culture fluid or required inoculating cell suspension is comparatively large, so need to apply the larger pipeline of internal diameter as DN25 or DN40 etc.The charging line of traditional bio-reactor adopts a pipeline directly to be passed into tank base by tank body top usually, if according to this traditional liquid feeding mode, there are the following problems for possibility: 1) tank interior can increase the position being difficult to clean, and may form cleaning dead angle; 2) after liquid feeding, the inside of charging line may retain part debris and form dead band, effectively cannot merge with the cell outside charging line; 3) because tank body is longer, if charging line directly passes into tank base from tank body top, comparatively difficulty is installed.In addition, if directly add cell culture fluid or inoculating cell suspension from tank body top, more, larger bubble can be produced again, and the shearing force produced during bubbles burst to cell damage, even can may cause necrocytosis.
Summary of the invention
The object of this invention is to provide a kind of modified version bio-reactor, by arranging the charging line of improvement, realize adherent liquid feeding mode, solve in prior art and adopt when carrying out liquid feeding by the tank body top charging line directly passed into tank base that existing tank interior exists clean dead angle, charging line inside exists debris, pipelining difficulty and to problems such as cell damage.Technical scheme provided by the present invention is: a kind of modified version bio-reactor, comprise tank body and charging line, described charging line is fixed on the top of described tank body, and the fluid inlet of described charging line stretches out outside described tank body, the liquid outlet of described charging line is positioned at described tank body, and the liquid outlet of described charging line is towards the inwall of described tank body, is also arranged with two heating modules in described tank body, described tank body is placed in base, and described base bottom is provided with wheel.
As preferably, described charging line comprises: straight sections, is fixed on the side wall upper part of described tank body, and the fluid inlet of the first end of described straight sections stretches out outside described tank body, and the second end of described straight sections stretches in described tank body; Bent portion, the first end of described bent portion docks with the second end of described straight sections, and the liquid outlet of the second end of described bent portion is towards the inwall of described tank body.
As preferably, described straight sections is horizontally placed on the side wall upper part of described tank body; Described bent portion is semicircular bend pipe structure.
As preferably, described bent portion and described straight sections connect as one structure.
Compared with prior art, beneficial effect of the present invention is as follows: nutrient solution can enter in tank body by charging line, because the liquid outlet of charging line is towards inner tank wall, during liquid feeding, nutrient solution along inner tank wall to tank base stream, can effectively reduce the generation of bubble; In addition, modified version bio-reactor of the present invention is convenient mobile.
Accompanying drawing explanation
Fig. 1 represents the structural representation of modified version bio-reactor of the present invention.
Embodiment
Be described principle of the present invention and feature below in conjunction with accompanying drawing, example, only for explaining the present invention, is not intended to limit scope of the present invention.
In prior art, the charging line of modified version bio-reactor normally adopts a pipeline directly to stretch to tank base by tank body top, such structure can exist when liquid feeding produce bubble many and to cell produce injury, can be formed in clean dead angle, pipeline can retain debris and install difficulty etc. problem.
Modified version bio-reactor of the present invention, can realize adherent liquid feeding mode, produces bubble when reducing liquid feeding.Refer to Fig. 1, modified version bio-reactor of the present invention comprises tank body 2 and charging line 1, wherein, charging line 1 is fixed on the top of tank body 2, and the fluid inlet 1a of charging line 1 stretches out outside tank body 2, the liquid outlet 1b of charging line 1 is positioned at tank body 2, and the liquid outlet 1b of charging line 1 is towards the inwall of tank body 2.
Adopt the modified version bio-reactor of this structure, nutrient solution can enter in tank body 2 by charging line 1, liquid outlet 1b due to charging line 1 is the inwall facing toward tank body 2, therefore, during liquid feeding, nutrient solution can flow bottom tank body 2 along tank body 2 inwall, and this adherent liquid feeding mode can reduce the generation of bubble effectively, thus avoids cell to be subject to the injury of the shearing force that bubbles burst produces.
In addition, in the present invention, be also arranged with two heating modules 3, can regulate the temperature in tank body 2 in described tank body 2, described tank body 1 is placed in base 4, and described base 4 bottom is provided with wheel 5, convenient mobile.In the present invention, described charging line comprises: straight sections, is fixed on the side wall upper part of described tank body, and the fluid inlet of the first end of described straight sections stretches out outside described tank body, and the second end of described straight sections stretches in described tank body; Bent portion, the first end of described bent portion docks with the second end of described straight sections, and the liquid outlet of the second end of described bent portion is towards the inwall of described tank body.
In the preferred embodiment of the present invention, the structure that bent portion and straight sections can be integrated, structure is simple, easy processing and manufacturing.
Protection scope of the present invention includes but not limited to above embodiment, and protection scope of the present invention is as the criterion with claims, and any replacement that those skilled in the art will find apparent that, distortion, improvement made this technology all falls into protection scope of the present invention.