技术领域technical field
本发明属于医用设备技术领域，具体涉及一种富血小板血浆专用离心管。The invention belongs to the technical field of medical equipment, and in particular relates to a special centrifuge tube for platelet-rich plasma.
背景技术Background technique
正常生物组织都具有一定的自身修复能力，而生物组织的修复能力主要是通过生长因子实现的。富血小板血浆(platelet rich plasma，PRP)是一种将组织自身修复能力调动起来为生物体自身修复所用的技术。PRP是通过离心的方法从全血中提取出来的血小板浓缩液，其含有高浓度的血小板、白细胞和纤维蛋白。血小板激活后能分泌多种生长因子，白细胞可防止感染，纤维蛋白能在局部构建组织修复所需的三维结构。浓缩的这些PRP成分不仅仅为组织的修复提供了浓缩的营养，还为组织修复搭建了更好的修复环境，能够促进骨组织和软组织的修复。Normal biological tissues have a certain self-repair ability, and the repair ability of biological tissues is mainly realized by growth factors. Platelet rich plasma (PRP) is a technology that mobilizes the self-repair ability of tissues for the self-repair of organisms. PRP is a platelet concentrate extracted from whole blood by centrifugation, which contains high concentrations of platelets, white blood cells and fibrin. After activation, platelets can secrete a variety of growth factors, white blood cells can prevent infection, and fibrin can locally build the three-dimensional structure required for tissue repair. The concentrated PRP components not only provide concentrated nutrition for tissue repair, but also build a better repair environment for tissue repair, which can promote the repair of bone tissue and soft tissue.
PRP在临床上用于组织修复只有十余年的历史，但由于PRP来源于自体制作简单，且安全有效，近几年在临床上的应用日益广泛，特别是在欧美国家，现已应用到骨科、空前额面外科、神经外科、眼科、耳鼻喉科和整形美容科等多门学科。在中国，目前相对于PRP的研究和临床应用还很少：一是因为国内还没有制作PRP的专门设备；二是因为国内医生对PRP还知之甚少。PRP has only been used clinically for tissue repair for more than ten years. However, because PRP is derived from autologous materials, it is easy to make, safe and effective. In recent years, it has been widely used in clinical practice, especially in European and American countries. , unprecedented frontal surgery, neurosurgery, ophthalmology, ENT and plastic surgery and other disciplines. In China, there are few researches and clinical applications relative to PRP: first, because there is no special equipment for making PRP in China; second, because domestic doctors still know little about PRP.
目前，国内大多采用手工二次离心法制备PRP，存在以下缺点极大地限制了其在临床上的应用：1)容易受到外界污染：多个容器中转移，增加了血小板激活和被污染的机会；2)PRP层为薄薄一层，抽取时易受操作因素影响，血小板收率浮动很大；3)离心管使用不方便，操作复杂，难度大。At present, most of the domestic PRP is prepared by manual secondary centrifugation, which has the following disadvantages that greatly limit its clinical application: 1) It is easy to be contaminated by the outside world: transfer in multiple containers increases the chance of platelet activation and contamination; 2) The PRP layer is a thin layer, which is easily affected by operational factors during extraction, and the yield of platelets fluctuates greatly; 3) The centrifuge tube is inconvenient to use, and the operation is complicated and difficult.
发明内容Contents of the invention
针对上述现有技术中存在的问题，本发明的目的在于提供一种可避免出现上述技术缺陷的富血小板血浆专用离心管，只需要一次离心就可以得到血小板富集率高、破裂少的富血小板血浆。In view of the problems existing in the above-mentioned prior art, the object of the present invention is to provide a platelet-rich plasma special centrifuge tube that can avoid the above-mentioned technical defects, and only need one centrifugation to obtain platelet-rich plasma with high platelet enrichment rate and less rupture plasma.
为了实现上述发明目的，本发明采用的技术方案如下：In order to realize the foregoing invention object, the technical scheme that the present invention adopts is as follows:
一种富血小板血浆专用离心管，为密闭结构，包括管体12、旋盖3、双漏斗形隔板7、调节手柄11和锥形活塞10，其中：A special centrifuge tube for platelet-rich plasma, with a closed structure, including a tube body 12, a screw cap 3, a double funnel-shaped partition 7, an adjustment handle 11 and a tapered piston 10, wherein:
所述旋盖3设置在所述管体12的上端；The screw cap 3 is arranged on the upper end of the tube body 12;
所述调节手柄11设置在所述管体12的下端；The adjusting handle 11 is arranged at the lower end of the pipe body 12;
所述锥形活塞10设置在所述管体12的下端的内部，且与所述调节手柄11相连接；The tapered piston 10 is arranged inside the lower end of the tubular body 12 and connected with the adjusting handle 11;
所述双漏斗形隔板7设置在所述管体12的中部的内部。The double funnel-shaped partition 7 is arranged inside the middle part of the pipe body 12 .
进一步地，所述旋盖3上设置有封闭的注样口1和取样口2。Further, the screw cap 3 is provided with a closed sample injection port 1 and a sample port 2 .
进一步地，所述双漏斗形隔板7由开口向下漏斗6和开口向上漏斗8连接而成。Further, the double funnel-shaped partition 7 is formed by connecting a funnel 6 with an opening downward and a funnel 8 with an upward opening.
进一步地，所述开口向下漏斗6的顶部设置有标管4。Further, a standard pipe 4 is provided on the top of the funnel 6 with an opening downward.
进一步地，所述标管4上设置有标线5。Further, the standard tube 4 is provided with a marking line 5 .
进一步地，所述开口向上漏斗8的底部设置有止逆阀9。Further, a check valve 9 is provided at the bottom of the upward funnel 8 .
本发明提供的富血小板血浆专用离心管，具有如下增益效果：1)全密闭结构，降低血小板激活和被污染的几率，提高PRP质量；2)双漏斗形隔板，可以使离心过程中的血小板和红细胞等有效透过隔板，沉积在离心管下层，止逆阀的存在可以防止血小板层和红细胞层回流；3)离心完成后，只需轻旋调节手柄，将红细胞层推挤至标管的标线处，抽取标管内血浆，即为高质量PRP，避免了人为操作因素引起的血小板收率浮动问题，可以很好地满足实际应用的需要。The special centrifuge tube for platelet-rich plasma provided by the present invention has the following beneficial effects: 1) fully enclosed structure, which reduces the probability of platelet activation and contamination, and improves the quality of PRP; 2) double funnel-shaped partitions, which can make the platelet and red blood cells effectively pass through the partition and deposit in the lower layer of the centrifuge tube. The existence of the check valve can prevent the backflow of the platelet layer and red blood cell layer; 3) After the centrifugation is completed, just turn the adjustment handle lightly to push the red blood cell layer to the standard tube At the marked line, the plasma in the standard tube is extracted, which is high-quality PRP, which avoids the fluctuation of platelet yield caused by human operation factors, and can well meet the needs of practical applications.
附图说明Description of drawings
图1为本发明的结构示意图；Fig. 1 is a structural representation of the present invention;
图2为本发明的顶视图；Fig. 2 is the top view of the present invention;
图中，1-注样口，2-取样口，3-旋盖，4-标管，5-标线，6-开口向下漏斗，7-双漏斗形隔板，8-开口向上漏斗，9-止逆阀，10-锥形活塞，11-调节手柄。In the figure, 1-sample injection port, 2-sampling port, 3-screw cap, 4-standard tube, 5-marking line, 6-opening downward funnel, 7-double funnel-shaped partition, 8-opening upward funnel, 9-check valve, 10-tapered piston, 11-regulating handle.
具体实施方式detailed description
下面结合附图和具体实施例对本发明做进一步说明。The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
如图1和图2所示，一种富血小板血浆(PRP)专用离心管，具有密闭结构，包括管体12、旋盖3、双漏斗形隔板7、调节手柄11和锥形活塞10，其中：所述旋盖3设置在所述管体12的上端；所述调节手柄11设置在所述管体12的下端；所述锥形活塞10设置在所述管体12的下端的内部，且与所述调节手柄相连接；所述双漏斗形隔板7设置在所述管体12的中部的内部。所述旋盖3上设置有封闭的注样口1和取样口2。所述双漏斗形隔板7由开口向下漏斗6和开口向上漏斗8连接而成。所述开口向下漏斗6的顶部设置有标管4。所述标管4上设置有标线5。所述开口向上漏斗8的底部设置有止逆阀9。下方的调节手柄11与锥形活塞10相连接，可通过调节手柄11的旋转实现锥形活塞10的前进与后退。As shown in Figures 1 and 2, a special centrifuge tube for platelet-rich plasma (PRP) has a closed structure, including a tube body 12, a screw cap 3, a double funnel-shaped partition 7, an adjustment handle 11 and a tapered piston 10, Wherein: the screw cap 3 is arranged on the upper end of the tubular body 12; the adjusting handle 11 is arranged on the lower end of the tubular body 12; the tapered piston 10 is arranged inside the lower end of the tubular body 12, And connected with the adjustment handle; the double funnel-shaped partition 7 is arranged inside the middle part of the pipe body 12 . The screw cap 3 is provided with a closed sample injection port 1 and a sample port 2 . The double funnel-shaped partition 7 is formed by connecting a funnel 6 with an opening downward and an upward funnel 8 with an opening. A standard pipe 4 is arranged on the top of the funnel 6 with the opening downward. The standard tube 4 is provided with a marking line 5 . A check valve 9 is provided at the bottom of the upward funnel 8 . The adjusting handle 11 below is connected with the conical piston 10, and the forward and backward movement of the conical piston 10 can be realized by the rotation of the adjusting handle 11.
富血小板血浆(PRP)专用离心管的旋盖3的外径范围为30-55mm，优选为45mm；内径范围为25-50mm，优选为40mm；注样口1和取样口2的直径范围为2-8mm，优选为4mm；The outer diameter range of the screw cap 3 of the platelet-rich plasma (PRP) special centrifuge tube is 30-55mm, preferably 45mm; the inner diameter range is 25-50mm, preferably 40mm; the diameter range of the injection port 1 and the sampling port 2 is 2 -8mm, preferably 4mm;
富血小板血浆(PRP)专用离心管的管体12的直径范围为30-50mm，优选为40mm；壁厚为0.8-2mm，优选为1.2mm；管体12的高度为150-250mm，优选为170mm；The diameter range of the tube body 12 of platelet-rich plasma (PRP) special centrifuge tube is 30-50mm, preferably 40mm; the wall thickness is 0.8-2mm, preferably 1.2mm; the height of the tube body 12 is 150-250mm, preferably 170mm ;
富血小板血浆(PRP)专用离心管的管体12中的双漏斗形隔板7位于富血小板血浆(PRP)专用离心管的管体12中部；The double funnel-shaped partition 7 in the tube body 12 of the platelet-rich plasma (PRP) special centrifuge tube is located in the tube body 12 middle part of the platelet-rich plasma (PRP) special centrifuge tube;
双漏斗形隔板7的下部的止逆阀9的直径为2-6mm，优选为4mm；上部的标管4的直径为2-6mm，优选为4mm；标管4的高度为20-50mm，优选为30mm；标管4的壁厚为0.2-1.0mm，优选为0.8mm；标线5位于距离标管4的底端3/4标线5长度的位置处；The diameter of the check valve 9 at the bottom of the double funnel-shaped dividing plate 7 is 2-6mm, preferably 4mm; the diameter of the standard pipe 4 on the top is 2-6mm, preferably 4mm; the height of the standard pipe 4 is 20-50mm, Preferably 30mm; the wall thickness of the standard tube 4 is 0.2-1.0mm, preferably 0.8mm; the marking line 5 is located at the position of the 3/4 marking line 5 length from the bottom end of the standard tube 4;
富血小板血浆(PRP)专用离心管的调节手柄11的外径范围为30-55mm，优选为45mm；内径范围为25-50mm，优选为40mm；调节手柄11的高度为20-60mm，优选为40mm；The outer diameter range of the adjustment handle 11 of the platelet-rich plasma (PRP) special centrifuge tube is 30-55mm, preferably 45mm; the inner diameter range is 25-50mm, preferably 40mm; the height of the adjustment handle 11 is 20-60mm, preferably 40mm ;
富血小板血浆(PRP)专用离心管的锥形活塞10与调节手柄11相连接，可通过调节手柄11的旋转实现锥形活塞10的前进与后退；锥形活塞10的外径的尺寸与富血小板血浆(PRP)专用离心管的管体12的内径相等，优选为40mm。The conical piston 10 of the special centrifuge tube for platelet-rich plasma (PRP) is connected with the adjustment handle 11, and the advancement and retreat of the conical piston 10 can be realized by the rotation of the adjustment handle 11; The inner diameter of the tube body 12 of the special centrifuge tube for plasma (PRP) is equal, preferably 40mm.
本发明的使用步骤如下：The use steps of the present invention are as follows:
1)将30-60ml含有抗凝剂的新鲜血液缓慢注入到富血小板血浆(PRP)专用离心管内；1) Slowly inject 30-60ml of fresh blood containing anticoagulant into a special centrifuge tube for platelet-rich plasma (PRP);
2)将富血小板血浆(PRP)专用离心管放入离心机中，并放入配重使其平衡；2) Put the platelet-rich plasma (PRP) special centrifuge tube into the centrifuge, and put in the counterweight to make it balance;
3)男性血液1600G离心200s，女性血液1450G离心200s；3) Male blood was centrifuged at 1600G for 200s, and female blood was centrifuged at 1450G for 200s;
4)离心完成后，轻旋调节手柄11，通过锥形活塞10将红细胞层推挤至标管4的标线5处，抽取标管4内的血浆，即得到高质量富血小板血浆(PRP)。4) After the centrifugation is completed, turn the adjustment handle 11 lightly, push the red blood cell layer to the marking line 5 of the standard tube 4 through the conical piston 10, and extract the plasma in the standard tube 4 to obtain high-quality platelet-rich plasma (PRP) .
本发明提供的富血小板血浆专用离心管，具有如下增益效果：1)全密闭结构，降低血小板激活和被污染的几率，提高PRP质量；2)双漏斗形隔板，可以使离心过程中的血小板和红细胞等有效透过隔板，沉积在离心管下层，止逆阀的存在可以防止血小板层和红细胞层回流；3)离心完成后，只需轻旋调节手柄，将红细胞层推挤至标管的标线处，抽取标管内血浆，即为高质量PRP，避免了人为操作因素引起的血小板收率浮动问题，可以很好地满足实际应用的需要。The special centrifuge tube for platelet-rich plasma provided by the present invention has the following beneficial effects: 1) fully enclosed structure, which reduces the probability of platelet activation and contamination, and improves the quality of PRP; 2) double funnel-shaped partitions, which can make the platelet and red blood cells effectively pass through the partition and deposit in the lower layer of the centrifuge tube. The existence of the check valve can prevent the backflow of the platelet layer and red blood cell layer; 3) After the centrifugation is completed, just turn the adjustment handle lightly to push the red blood cell layer to the standard tube At the marked line, the plasma in the standard tube is extracted, which is high-quality PRP, which avoids the fluctuation of platelet yield caused by human operation factors, and can well meet the needs of practical applications.
以上所述实施例仅表达了本发明的实施方式，其描述较为具体和详细，但并不能因此而理解为对本发明专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进，这些都属于本发明的保护范围。因此，本发明专利的保护范围应以所附权利要求为准。The above-mentioned embodiments only express the implementation manner of the present invention, and the description thereof is relatively specific and detailed, but should not be construed as limiting the patent scope of the present invention. It should be pointed out that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510607177.2ACN105289772B (en) | 2015-09-22 | 2015-09-22 | A kind of special centrifuge tube of platelet rich plasma |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510607177.2ACN105289772B (en) | 2015-09-22 | 2015-09-22 | A kind of special centrifuge tube of platelet rich plasma |
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| CN105289772A CN105289772A (en) | 2016-02-03 |
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| CN201510607177.2AExpired - Fee RelatedCN105289772B (en) | 2015-09-22 | 2015-09-22 | A kind of special centrifuge tube of platelet rich plasma |
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| CN106669875B (en)* | 2017-02-13 | 2022-07-01 | 深圳市邦沃科技有限公司 | Platelet-rich plasma centrifuge tube and extraction method thereof |
| CN114146827B (en)* | 2021-11-26 | 2024-01-23 | 南京双威生物医学科技有限公司 | One-time centrifugation preparation method of platelet-rich plasma |
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