A kind of microbe rapid detection device based on Mie scattering and spatial light modulatorTechnical field
It is a kind of based on Mie scattering and spatial light the invention belongs to medical biotechnology microorganism, cell tissue detection fieldThe microbe rapid detection device of modulator.
Background technique
Microorganism is all tiny organisms that are invisible or not seeing, and individual is small, and structure is simple, usually to use upThe biology that microscope and electron microscope can just be seen is learned, microorganism is referred to as.Microorganism includes bacterium, virus, mould, fermentFemale bacterium etc..The detection of microorganism has important meaning in terms of food safety, medical diagnosis on disease and control, bioscienceJustice.The quick testing requirements of microorganism detect objective microbe, while sensitivity and specificity in the shortest possible timeHeight operates as simple as possible.Traditional microorganism detection method mainly have culture embrane method, spiral planar counting method, filter membrane method withAnd emerging ATP bioluminescence method, electrical impedance method, color change, Flow Cytometry and laser scanner technique.These methods are logicalChang Chengben is relatively high, needs to carry out a certain number of cultures to microorganism in advance and specific culture examination is added during the cultivation processAgent, while incubation needs to expend the more time, and a possibility that false positive occur higher for testing result.
Mie theory is in research transparent medium, and monochromatic optical wave is irradiated to the spheric granules of arbitrary diameter and ingredientOn, the theory of characteristic and solution that light wave scatters.Mie theory is led in environmental protection, the energy, astronomy, meteorology, medicine etc.Domain is widely applied.Microorganism in transparency liquid can issue scattering light after the irradiation of laser to surrounding space,And there are correlation, scattered light intensity of the particle of different shapes in space with particle diameter for the scattered light intensity in different angle spaceDifferent rules is distributed in degree, by collecting the scattering light of surrounding space, can solve microorganism based on Mie theoryShape-structure parameter.Method for rapid inspecting animalcule based on Mie scattering is not needed to additionally introduce chemical reagent, be obtained simultaneouslySignal data real-time levels are high；Versatility is good, and the microorganism detection of different shape and size may be implemented in set of device；Detection speedDegree is fast, since the method using optical detection can quickly obtain scattering light letter by the photoelectric detector of high-speedNumber, the form signals feature of present microorganism is then obtained by computer.
To realize the rapid microbial detection based on Mie scattering, need the monochromatic light exposure of certain energy to microorganismOn, it is the Ordinary Light Sources Have of current biological optical detection that laser, which has the characteristics that monochromaticjty is good, energy is concentrated, beam quality is high,.TogetherWhen scatter light for collection space, need to place photoelectric detector around microorganism, photomultiplier tube has response speedFastly, the feature that noise is low, gain is big is the common device in photoelectric detection system.
In conclusion the microbial rapid detection system based on Mie scattering has wide utilization prospect, compared to biographyThe microorganism detection method of system can greatly improve the speed and precision of detection, and easy to operate, versatile.
Summary of the invention
The purpose of the present invention is overcoming the shortcomings of the prior art, one kind is provided and easily realizes that quick real-time detection is micro-The Systems for optical inspection structure of biomorph.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of microbe rapid detection device based on Mie scattering and spatial light modulator, including laser, optical systemSystem, detector ball array frame, sample cell, optical fiber, spatial light modulator, photomultiplier tube；Laser generates power adjustableContinuous laser, after optical system converges, by the hole of detector ball array frame port be irradiated to detector spherical shapeArray frame center, laser facula focus point are overlapped with the centre of sphere of detector ball array frame, in detector ball array frameSample cell is placed in frame, sample cell is spherical shaped transparent glassware, and the sample cell centre of sphere and the detector ball array frame centre of sphere are totalThe heart is overlapped, and on the sample that laser convergence is irradiated in sample cell, the scattering light of sample is distributed on detector ball array frameOn optical fiber collect, signal light by fiber-optic illuminated in spatial light modulator, by photoelectricity after spatial light modulator is modulatedMultiplier tube receives.
The detector ball array frame is combined by more semicircular arc-shaped metal structure axis and forms spherical cage structure,On semicircular arc-shaped metal structure axis, spaced set installs hole location, for installing optical fiber.
The optical fiber is mounted in the installation hole location of detector ball array frame, and the optical axis extended line of optical fiber intersects atThe centre of sphere of detector ball array frame.
The spatial light modulator fast implements the modulation to signal light, so that only allowing the signal of an optical fiber every timeThen light is handled by spatial light modulator by photomultiplier tube collection, acted on by the fast modulation of spatial light modulator, according toIt is secondary to realize that each scattered signal light is quickly handled by photomultiplier tube collection one by one.
The equipment further includes the computer for handling the received photooptical data of photomultiplier tube.
Microbe rapid detection device according to claim 1 based on Mie scattering and spatial light modulator, meterCalculation machine, which is adopted, to be shown a C language algorithm and handles photosignal, and the shape information of microorganism to be detected is obtained.
The algorithm is that MIE scatters intelligent Recursive method.
The method for quickly detecting microbial morphology using equipment of the present invention, comprising the following steps:
(1) known microorganisms are identified using the equipment, collects and scatters light characteristic information data, known to foundationMicrobial information database；The scattering light characteristic information includes scattered light intensity, scattering corner characteristics in all angles；In order toThe accuracy of the method for the present invention is improved, the information in database answers the scattering light as much as possible comprising known various microorganism belonging to genusCharacteristic information data；
(2) tested microorganism is measured using the equipment, by the scattering light characteristic information data and number of acquisitionBe compared according to the data in library, find with the most matched database information of calculated result, pass through the available sample cell of comparisonIn microorganism to be checked form information.
The utility model has the advantages that
1, using spherical cage structure, it can sufficiently collect the space scattering light light intensity for reacting microbial morphology to be checked and dissipateFiring angle, while the axis of spherical cage structure can do appropriate extension, and according to different detection targets and requirement, can satisfy differenceStructural requirement.2, versatile, different microorganisms does not need to introduce additional detection reagent, realizes that set of device can be examinedSurvey plurality of target microorganism.
3, detection speed is fast, and quick photoelectric signal transformation may be implemented in photomultiplier tube, and gain of photomultiplier is high,The detection to tiny signal may be implemented, be conducive to the detection sensitivity of raising system.It can finally be obtained via high-speed computerTo real-time testing result.
4, detector ball array frame, microorganism to be detected is overlapped with the detector ball array frame centre of sphere, so that lightFibre can uniformly collect scattered light signal, improve detection accuracy.
5, spatial light modulator, can fast implement the modulation to light beam, and in the present invention, multi beam scattered light signal passes through lightFibre, which converges, to be irradiated in spatial modulator, is in the arrangement of certain rule by optical fiber, can to scatter light in consistent relationship photographIt is mapped in spatial light modulator.Then it is acted on by the fast modulation of spatial light modulator, such as attached drawing 2, primary can be madeThere is an optical signal to reach photomultiplier tube by spatial light modulator, is subsequently can by computer.It is sequentially modulated so that each is believedIt is processed number to pass sequentially through spatial light modulator one by one.
Detailed description of the invention
Fig. 1 is the microbe rapid detection device schematic diagram based on Mie scattering proposed according to the present invention.
Wherein, 1 is laser, and 2 be optical system, and 3 be detector ball array frame, and 4 be sample cell, and 5 be optical fiber, 6It is photomultiplier transit light for spatial light modulator, 7.
Fig. 2 is spatial light modulator modulation scattered light signal schematic diagram.
Specific embodiment
Present invention is further described in detail with reference to the accompanying drawing.
In conjunction with Fig. 1, the microbe rapid detection device based on Mie scattering is mainly by laser (1), optical system (2),Detector ball array frame (3), sample cell (4), optical fiber (5) composition.The diameter for surveying device ball array frame (3) is 203mm,Single semicircular arc-shaped axis with a thickness of 26mm, detector ball array frame (3) is to be made of four identical semicircular arc-shaped axisSpherical cage structure, the optical maser wavelength of laser (1) is 632nm, and power is that 0mW to 100mW is adjustable, laser (1) transmittingLaser out is converged by optical system (2), the focus of optical system (2) and the centre of sphere of detector ball array frame (3)It is overlapped, the back focal plane of optical system (2) is 260mm, and the sample cell (4) of glass marble shape is placed in detector ball array frame(3) in, the centre of sphere of sample cell (4) is overlapped with the centre of sphere of detector ball array frame (3), and the bulb diameter of sample cell (4) is79mm, 28 optical fiber (5) of equidistant distributing installation on the axis of detector ball array frame (3), the optical axis of optical fiber (5)The centre of sphere of extended line directed towards detector ball array frame (3), and the centre of sphere of detector ball array frame (3) is met at simultaneously.The other end of 28 optical fiber (5) merges in spatial light modulator (6) front end, by spatial light modulator (6) modulated signalLight is received by photomultiplier tube (7), is converted into electric signal.
The principle of device is as follows: being the directional light after collimation by the laser that laser (1) emits, directional light passes through lightAfter system (2) convergence, the focal point of optical system (2) is focused on, due to the focus and detector spherical array of optical system (2)The centre of sphere of column frame (3) and sample cell (4) is overlapped, and laser beam focuses on sample cell (4) after optical system (2) are convergedAt the centre of sphere, the microbiological specimens in sample cell (4) can launch scattering light to surrounding space due to the irradiation by laser, emptyBetween all directions scattering by corresponding optical fiber (5) receive collect, via spatial light modulator (6) modulation after by photomultiplier tube(7) it receives and is converted to electric signal, stored by computer.
Later period carries out processing analysis to obtained photosignal by computer, passes through the scattering light light of each sensing pointBy force, it is updated to Mie scattering solution formula, is compared, is looked for known microorganisms information database by obtained calculated resultTo with the most matched database information of calculated result, pass through the form of the microorganism to be checked in the available sample cell (4) of comparisonInformation.
For identifying whether unknown microorganism is Escherichia coli, illustrate specific method and result of the invention: (1) basisAbovementioned steps 1 establish Escherichia coli information database, i.e., the Escherichia coli obtained pure culture are put into microbial rapid detection dressIn setting, the characteristics such as scattered light intensity, the angle of scattering in all angles are collected using the device.(2) by unknown microorganism intoIt is put into after row pure culture in microbial rapid detection device, collects scattered light intensity in all angles, scattering using the deviceThe characteristic being collected into is compared with Escherichia coli information database, obtains the unknown microorganism by the characteristics such as angleFor the probability value of Escherichia coli.
The present invention is described in detail above, but the invention is not restricted to the embodiments described.It is all according to the present inventionEquivalent change or modification made by Spirit Essence, should be covered by the scope of protection of the present invention.