Ram pump application of sample dyeing assembly and overflow dyeing machineTechnical field
The present invention relates to cell pathology detection technique, particularly relate to ram pump application of sample dyeing assembly and overflow dyeing machine.
Background technology
Liquid based cytology test (Liquid-BasedCytologyTest is called for short " LCT ") technology is mainly used in cervical cytology and detects, and is particularly useful for the examination of cervical carcinoma.
Its principle of work is: be also all eluted to special sampler scraping cervical exfoliated cell and fill in the plastic bottle of cell-preservation liquid, deliver to laboratory, cell mass depolymerization is made after vibration rod is even, conserving liquid containing cell specimen is through centrifugation, make the magazins' layout such as cell fragment, blood, mucus and the leucocyte in cervical epithelial cells wherein and sample, obtain pure cervical epithelial cells, by cell transfer techniques, cervical epithelial cells is transferred to thin-layer cell layer microslide being made diameter 13mm, then rinse dyeing.These processes have controlled automatically by system, can be called to complete under the work of overflow dyeing machine, finally by diagosis make diagnosis by TBS under the microscope after technician's mounting.
Advantage is: the cellular layer made by LCT technology becomes monolayer distribution, clear background, is convenient to observe, and loss is low.
External LCT technology is based on AutoCyto (U.S. company BD production), although this checkout equipment slice-making quality is effective, price is higher, and Expenses of laboratory examination is expensive, and maintenance not in time, is not suitable for large-scale popularization at home.In the recent period, more domestic companies also develop the liquid basal cell thin layer dyeing machine of applicable national conditions, as the LCT checkout equipment (application number is a kind of dyeing machine of 2007100272504 and the method for film-making dyeing) of Guangzhou An Biping company, but from clinical test results, still there is very big-difference compared with external LCT technology, slice-making quality differs greatly.
The instrument of film-making and reagent all can produce certain impact to production effect, for automatic staining machine, mainly come from dropping liquid, leakage causes originally dyed cell again to be washed off dyeing by other dye liquor, film-making occurs that part coloured portions does not contaminate, be exactly so-called negative and positive sheet, in addition, if there is instrument failure in film-making process, all film-makings can be caused to interrupt, cause film-making failure.When using syringe application of sample, syringe fragmentation, line clogging or leakage (accessory is many, interface is many) are the one of the main reasons causing instrument failure.And dropping liquid, leakage mainly application of sample process occur, or motor drive mode causes.Peristaltic pump application of sample in the past, because the conduit of peristaltic pump is squeezed for a long time, make conduit easily aging, peristaltic pump cannot be closed completely, causes pin mouth place liquid drippage; And use syringe application of sample, affect sealing property by difference variation, cause the phenomenon of leakage.And the type of drive of even acceleration in the past is mainly formed by numerous little accelerator set, when in fact especially completing acceleration or slow down in motion process, due to the effect of inertia, larger shake can be caused, the liquid remained in sample needle is shaken off.
To sum up, current LCT technology is comparatively ripe, automatic staining machine on market, all adopt syringe to carry out application of sample, the material of syringe all adopts glass, during deisgn product, all outside instrument cabinet, glass belongs to fragile article exposed for syringe, therefore in production, transport and use procedure, easily causes syringe breakage because of the reason such as colliding, the temperature difference is large; And instrument adopts syringe application of sample, the demand that multiple accessory could meet instrument working procedure must be assembled, the weight of instrument and space can be increased like this and cause assembly process loaded down with trivial details; Market also once there is the device of peristaltic pump application of sample, but be squeezed for a long time due to conduit, and made conduit easily aging, shorten the serviceable life of product, and also can produce the phenomenon of hanging liquid or water clock liquid simultaneously.
Summary of the invention
In order to overcome the deficiencies in the prior art, an object of the present invention is to provide ram pump application of sample dyeing assembly, can simplify structure, and avoid hanging liquid or leakage.
Two of object of the present invention is to provide the overflow dyeing machine adopting described ram pump application of sample dyeing assembly.
An object of the present invention realizes by the following technical solutions:
Ram pump application of sample dyeing assembly, comprise staining bottle, damping fluid bottle, dyeing pin, sample needle, waste liquid draw-out device, the first ram pump, the second ram pump, the first connector, some first two-bit triplet solenoid valves and the second two-bit triplet solenoid valve, first ram pump connects the common port of each the first two-bit triplet solenoid valve respectively by the first connector, the normal-closed end of each the first two-bit triplet solenoid valve connects one to one dyeing pin, and staining bottle connects the Chang Kaiduan of each the first two-bit triplet solenoid valve respectively; Second ram pump connects the common port of the second two-bit triplet solenoid valve, and the normal-closed end of the second two-bit triplet solenoid valve connects sample needle, and the Chang Kaiduan of the second two-bit triplet solenoid valve connects damping fluid bottle; Waste liquid draw-out device comprises the waste liquid of dyeing liquor and damping fluid for extracting.
Further, waste liquid draw-out device comprises some waste liquid pins, the second connector and waste drains pump, and one end of each waste liquid pin is all connected to waste drains pump by the second connector.
Two of object of the present invention realizes by the following technical solutions:
Adopt the overflow dyeing machine of described ram pump application of sample dyeing assembly, comprise worktable and be all installed on this worktable for the just right scratch diskette of dyeing pin, for the specimen holder just right with sample needle, for the suction nozzle seat just right with sample needle and four-axle linked mechanism, four-axle linked mechanism has the X-axis slipping mechanism along the slippage of worktable X-direction, be installed on X-axis slipping mechanism and along the Y-axis slipping mechanism of worktable Y direction slippage, be installed on Y-axis slipping mechanism and along the slippage of worktable Z-direction Z1 axle slipping mechanism and be installed on Y-axis slipping mechanism and the Z2 axle slipping mechanism be slidably matched along worktable Z-direction, sample needle is installed on Z1 axle slipping mechanism, dyeing pin is installed on Z2 axle slipping mechanism.
Further, X-axis slipping mechanism comprises X-axis slide rail and X-axis driving mechanism, Y-axis slipping mechanism comprises Y-axis slide rail and Y-axis driving mechanism, Z1 axle slipping mechanism comprises Z1 bracing strut and Z1 axle driving mechanism, Z2 axle slipping mechanism comprises Z2 bracing strut and Z2 axle driving mechanism, and Y-axis slide rail and X-axis slide rail are slidably matched, and Z1 bracing strut and Z2 bracing strut are slidably matched with Y-axis slide rail respectively, sample needle is installed on Z1 bracing strut, and dyeing pin is installed on Z2 bracing strut.
Further, worktable is also provided with for storing the waste product box of suction nozzle waste material and the waste liquid tank for flushing waste.
Compared to existing technology, beneficial effect of the present invention is:
First ram pump of the present invention, the second ram pump are integrated, to efficiently solve in production, transport and use procedure easily because collide, the temperature difference is large etc., and reason causes the problems such as breakage, decrease assembly space and operation, I&M is more convenient, simultaneously, break-make in conjunction with the first two-bit triplet solenoid valve, the second two-bit triplet solenoid valve controls to use, and also can not produce the phenomenon of hanging liquid or water clock liquid.
Accompanying drawing explanation
Fig. 1 is the dyeing fluid path schematic diagram of ram pump application of sample of the present invention dyeing assembly;
Fig. 2 is the application of sample fluid path schematic diagram of ram pump application of sample of the present invention dyeing assembly;
Fig. 3 is the devil liquor recovery fluid path schematic diagram of ram pump application of sample of the present invention dyeing assembly;
Fig. 4 is the front view of overflow dyeing machine of the present invention;
Fig. 5 is the vertical view of Fig. 4;
Fig. 6 is the left view of Fig. 4.
In figure: 1, staining bottle; 2, damping fluid bottle; 3, dye pin; 4, sample needle; 5, waste liquid pin; 6, the first ram pump; 7, the second ram pump; 8, the first connector; 9, the first two-bit triplet solenoid valve; 10, the second two-bit triplet solenoid valve; 11, waste product box; 12, the second connector; 13, waste drains pump; 14, worktable; 15, scratch diskette; 16, specimen holder; 17, suction nozzle seat; 18, four-axle linked mechanism; 181, X-axis slipping mechanism; 182, Y-axis slipping mechanism; 183, Z1 axle slipping mechanism; 184, Z2 axle slipping mechanism.
Embodiment
Below, by reference to the accompanying drawings and embodiment, the present invention is described further:
Ram pump application of sample dyeing assembly as shown in Figures 1 to 3, comprise staining bottle 1, damping fluid bottle 2, dyeing pin 3, sample needle 4, waste liquid draw-out device, the first ram pump 6, second ram pump 7, first connector 8, some first two-bit triplet solenoid valve 9 and the second two-bit triplet solenoid valves 10, first ram pump 6 connects the common port of each the first two-bit triplet solenoid valve 9 respectively by the first connector 8, the normal-closed end of each the first two-bit triplet solenoid valve 9 connects one to one dyeing pin 3, and staining bottle 1 connects the Chang Kaiduan of each the first two-bit triplet solenoid valve 9 respectively; Second ram pump 7 connects the common port of the second two-bit triplet solenoid valve 10, and the normal-closed end of the second two-bit triplet solenoid valve 10 connects the Chang Kaiduan connection damping fluid bottle 2 of sample needle 4, second two-bit triplet solenoid valve 10; Waste liquid draw-out device comprises the waste liquid of dyeing liquor and damping fluid for extracting, this waste liquid also includes the sample liquid added in preparation bin, is extracted reclaim with dyeing liquor, damping fluid after becoming waste liquid by waste liquid draw-out device.
First ram pump 6, second ram pump 7 is integrated, to efficiently solve in production, transport and use procedure easily because collide, the temperature difference is large etc., and reason causes the problems such as breakage, decrease assembly space and operation, I&M is more convenient, simultaneously, break-make in conjunction with the first two-bit triplet solenoid valve 9, second two-bit triplet solenoid valve 10 controls to use, and also can not produce the phenomenon of hanging liquid or water clock liquid.Wherein, in staining bottle 1, the dyeing liquor of splendid attire can be the one of damping fluid, washing fluid, haematoxylin, EA/OG, and damping fluid bottle 2 is for storing damping fluid.By controlling the energising of the first two-bit triplet solenoid valve 9, second two-bit triplet solenoid valve 10, just can stablize, effectively aspirate dyeing liquor, buffering enters corresponding dyeing pin 3 and sample needle 4, dyeing pin 3 correspondence connects damping fluid, washing fluid, haematoxylin, these four kinds of dyeing liquors of EA/OG, simultaneously, sample needle 4 is also connected to damping fluid, also namely, damping fluid is not only linked dyeing pin 3 but also is linked sample needle 4.For dividing into groups to each dyeing pin 3, to balance fluid injection, two the first two-bit triplet solenoid valves 9 can be divided into one group, accordingly, the first connector 8 can be set to the first three-way connection.
Specifically, during work, the second ram pump 7 rotates forward from translator, and the second two-bit triplet solenoid valve 10 no power work, is drawn onto damping fluid in ram pump; Then control the second ram pump 7 and carry motor reversal, control the second two-bit triplet solenoid valve 10 simultaneously and to be energized work, thus damping fluid is noted sample needle 4.The fluid injection principle of work of dyeing liquor is identical with the fluid injection principle of work of damping fluid, repeats no more herein.
Extract waste liquid for more convenient, waste liquid draw-out device comprises some waste liquid pin 5, second connectors 12 and waste drains pump 13, and one end of each waste liquid pin 5 is all connected to waste drains pump 13 by the second connector 12.Second connector 12 can be set to the second three-way connection, with uniform distribution liquor charging.
As shown in figures 4-6, adopt the overflow dyeing machine of described ram pump application of sample dyeing assembly, comprise worktable 14 and be all installed on this worktable 14 for the just right scratch diskette 15 of dyeing pin 3, for the specimen holder 16 just right with sample needle 4, for the suction nozzle seat 17 just right with sample needle 4 and four-axle linked mechanism 18, four-axle linked mechanism 18 has the X-axis slipping mechanism 181 of the X-direction slippage along worktable 14, be installed on X-axis slipping mechanism 181 and the Y-axis slipping mechanism 182 of Y direction slippage along worktable 14, be installed on Y-axis slipping mechanism 182 and along the Z-direction slippage of worktable 14 Z1 axle slipping mechanism 183 and be installed on Y-axis slipping mechanism 182 and the Z2 axle slipping mechanism 184 be slidably matched along the Z-direction of worktable 14, sample needle 4 is installed on Z1 axle slipping mechanism 183, dyeing pin 3 is installed on Z2 axle slipping mechanism 184, so, sample needle 4 can move along X, Y, Z1 axle, and dyeing pin 3 can move along X, Z2 axle.Waste liquid draw-out device acts on the devil liquor recovery of machine pipes inside, by be arranged on the waste drains pump of worktable and be arranged on Z1 axle slipping mechanism, the waste liquid pin of Z2 axle slipping mechanism realizes devil liquor recovery.。
Sample needle 4 enters specimen holder 16 again and carries out suction sample after drawing suction nozzle, arrive scratch diskette 15 application of sample again, four-axle linked mechanism 18 is respectively dyeing pin 3 and sample needle 4 provides the flexible locomotivity of three axles, with accurate alignment work dish 15, suction nozzle seat 17 and specimen holder 16 respectively.Can control device be coordinated, realize more full automatic tableting operation.
Exemplarily, X-axis slipping mechanism 181 comprises X-axis slide rail and X-axis driving mechanism, Y-axis slipping mechanism 182 comprises Y-axis slide rail and Y-axis driving mechanism, Z1 axle slipping mechanism 183 comprises Z1 bracing strut and Z1 axle driving mechanism, Z2 axle slipping mechanism 184 comprises Z2 bracing strut and Z2 axle driving mechanism, and Y-axis slide rail and X-axis slide rail are slidably matched, and Z1 bracing strut and Z2 bracing strut are slidably matched with Y-axis slide rail respectively, sample needle 4 is installed on Z1 bracing strut, and dyeing pin 3 is installed on Z2 bracing strut.Wherein, X-axis driving mechanism, Y-axis driving mechanism, Z1 axle driving mechanism, Z2 axle driving mechanism all can adopt band transmission to realize in conjunction with motor, certainly, can set up the precision that the elements such as shaft coupling, slide block, optoelectronic switch carry out sliding and lifting.
For convenience of Recycling of waste liquid, worktable 14 is also provided with for storing the waste product box 11 of suction nozzle waste material and the waste liquid tank for flushing waste.Waste product box 11 is for the waste liquid receiving application of sample and the suction nozzle waste material be finished, and the flushing process of dyeing liquor completes in waste liquid tank.
To one skilled in the art, according to technical scheme described above and design, other various corresponding change and deformation can be made, and all these change and deformation all should belong within the protection domain of the claims in the present invention.