技术领域technical field
本发明涉及的是一种基于微流体惯性效应的分选装置，尤其涉及的是一种低成本微米粒子浓缩装置及其制作方法。The invention relates to a sorting device based on the microfluidic inertial effect, in particular to a low-cost micron particle concentrating device and a manufacturing method thereof.
背景技术Background technique
浓缩技术是样品前处理过程中的重要方法，现已广泛应用于生物、医药、环境和食品等行业。样品浓缩在大幅度提高现有分析方法的检测能力起着非常关键的作用。目前主要的浓缩手段有：气流吹蒸法、减压浓缩法、旋转蒸发器浓缩法、真空离心浓缩法等。Concentration technology is an important method in the process of sample pretreatment, and has been widely used in biology, medicine, environment and food industries. Sample concentration plays a key role in greatly improving the detection capability of existing analytical methods. At present, the main concentration methods are: air blowing steaming method, vacuum concentration method, rotary evaporator concentration method, vacuum centrifugal concentration method, etc.
气流吹蒸法是将空气或氮气吹入盛有样品的容器中，不断降低液体表面蒸气压，使溶剂不断蒸发而达到浓缩的目的。此法虽操作简单，但效率低，并且仪器设备成本相对而言比较高昂。The steam blowing method is to blow air or nitrogen into the container containing the sample, continuously reduce the vapor pressure of the liquid surface, and continuously evaporate the solvent to achieve the purpose of concentration. Although this method is simple to operate, the efficiency is low, and the cost of equipment is relatively high.
有些待测组分对热不稳定，在较高温度下容易分解，采用减压浓缩，降低了溶剂的沸点，既可迅速浓缩至所需体积，又可避免被测物分解。常用的减压浓缩装置为全玻减压浓缩器，又称K-D浓缩器，这种仪器是一种常用的减压蒸馏装置，这种仪器浓缩净化液时具有浓缩温度低、速度快、损失少以及容易控制所需要体积的特点，适合对热不稳定被测物提取液的浓缩。但其缺点也比较明显，仪器只适合在实验室内进行操作，并且装置结构复杂，制作成本高，需要借助电气设备才能完成样品的浓缩。Some components to be tested are unstable to heat and are easy to decompose at higher temperatures. Concentration under reduced pressure reduces the boiling point of the solvent, which can quickly concentrate to the required volume and avoid the decomposition of the test substance. The commonly used vacuum concentrator is the all-glass vacuum concentrator, also known as the K-D concentrator. This instrument is a commonly used vacuum distillation device. This instrument has the advantages of low concentration temperature, fast speed and less loss when concentrating the purified liquid. And it is easy to control the required volume, which is suitable for the concentration of the extract solution of the thermally unstable analyte. But its disadvantages are also obvious. The instrument is only suitable for operation in the laboratory, and the structure of the device is complex, the production cost is high, and the concentration of the sample needs to be completed with the help of electrical equipment.
旋转蒸发器通过电子控制，使烧瓶在适宜的速度下旋转以增大蒸发面积。浓缩时可通过真空泵使蒸发烧瓶处于负压状态。盛装在蒸发烧瓶内的提取液，在水浴或油浴中加热的条件下，因在减压下边旋转、边加热，使蒸发瓶内的溶液黏附于内壁形成一层薄的液膜，进行扩散，增大了蒸发面积，并且，由于负压作用，溶剂的沸点降低，进一步提高了蒸发效率，同时，被蒸发的溶剂在冷凝器中被冷凝、回流至接收瓶。该法较一般蒸发装置蒸发效率成倍提高，并且可防止暴沸、被测组分氧化分解。但是在浓缩过程中复杂的操作步骤对被浓缩样品破坏比较大，不适用于对细胞等生物组织样品的浓缩，此法浓缩速度慢，操作麻烦。The rotary evaporator is electronically controlled to rotate the flask at a suitable speed to increase the evaporation area. When concentrating, the evaporation flask can be placed in a negative pressure state through a vacuum pump. The extract contained in the evaporating flask, under the condition of heating in a water bath or an oil bath, is rotated and heated under reduced pressure, so that the solution in the evaporating flask adheres to the inner wall to form a thin liquid film and diffuses. The evaporation area is increased, and, due to the negative pressure, the boiling point of the solvent is reduced, which further improves the evaporation efficiency. At the same time, the evaporated solvent is condensed in the condenser and returned to the receiving bottle. This method doubles the evaporation efficiency compared with general evaporation equipment, and can prevent bumping and oxidative decomposition of the measured components. However, the complex operation steps in the concentration process will cause great damage to the concentrated sample, and it is not suitable for the concentration of biological tissue samples such as cells. This method is slow in concentration and troublesome in operation.
真空离心浓缩就是采用离心机、真空和加热相结合的方法，在真空状态下离心样品，并通过超低温的冷阱捕捉溶剂，从而将溶剂快速蒸发达到浓缩或干燥样品的目的。离心浓缩后的样品可方便地用于各定性和定量分析定性和定量分析。但是其应用于生物微粒浓缩时，生物微粒机械性损伤较大，活性降低，对后续的分析可能会产生较大的误差。而且仪器设备复杂，便携性不好，不适合在户外、紧急情况下及时浓缩样品。Vacuum centrifugal concentration is a method of combining centrifuge, vacuum and heating to centrifuge the sample in a vacuum state, and capture the solvent through an ultra-low temperature cold trap, so as to quickly evaporate the solvent to achieve the purpose of concentrating or drying the sample. The sample after centrifugation and concentration can be conveniently used for various qualitative and quantitative analysis. However, when it is applied to the concentration of biological particles, the mechanical damage of biological particles is relatively large, and the activity is reduced, which may cause large errors in subsequent analysis. Moreover, the equipment is complex and the portability is not good, so it is not suitable for timely concentration of samples outdoors or in emergency situations.
弯流道惯性微流控技术利用粒子惯性迁移效应和弯曲率诱导产生的截面二次流来实现微粒子的高效操控，为微米粒子浓缩方法的发展提供了新的途径。弯流道中粒子在惯性升力和Dean拽力的共同作用下迁移至靠近流道内壁处，从而实现样品的浓缩。目前大多数微流控芯片材料采用聚二甲基硅氧烷（PDMS）、聚甲基丙烯酸甲酯（PMMA）、玻璃、硅或石英等聚合物和硬质材料来制作。不同的材料还需选择对应不同的加工工艺、键合技术。许多制作芯片步骤只能在专业的实验室内由专业的工作人员完成，从而难以实现微流控芯片的低成本化和商业化。The curved channel inertial microfluidic technology utilizes the particle inertial migration effect and the secondary flow induced by the curvature to achieve efficient manipulation of microparticles, which provides a new way for the development of micron particle concentration methods. Particles in the curved flow channel migrate to the inner wall of the flow channel under the joint action of inertial lift and Dean's drag force, so as to realize the concentration of the sample. At present, most microfluidic chip materials are made of polymers and hard materials such as polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), glass, silicon or quartz. Different materials need to be selected corresponding to different processing techniques and bonding techniques. Many chip making steps can only be completed by professional staff in professional laboratories, making it difficult to achieve low cost and commercialization of microfluidic chips.
发明内容Contents of the invention
发明目的：为克服现有技术不足，本发明旨于提供一种低成本微米粒子浓缩装置及其制作方法。Purpose of the invention: In order to overcome the deficiencies of the prior art, the present invention aims to provide a low-cost micron particle concentration device and its manufacturing method.
技术方案：为解决上述技术问题，本发明采用如下技术方案：Technical solution: In order to solve the above-mentioned technical problems, the present invention adopts the following technical solution:
一种低成本微米粒子浓缩装置，包括圆棒、橡胶层、螺旋圆管、接合器、Y型转接头、浓缩液出口微管和废液出口微管；所述螺旋圆管与橡胶层下边缘固连，螺旋圆管和橡胶层均匀地螺旋缠绕在圆棒外围；所述螺旋圆管顶端为进口端，底端为出口端；进口端与接合器连接，出口端与Y型转接头连接；所述Y型转接头为一进二出式结构，内部包括进液口、浓缩液出口、废液出口、第一连体接头、第二连体接头和第三连体接头，浓缩液出口和废液出口分别与进液口相通；所述进液口通过第一连体接头与出口端相通；浓缩液出口微管通过第二连体接头与浓缩液出口相通；废液出口微管通过第三连体接头与废液出口相通。A low-cost micron particle concentration device, including a round rod, a rubber layer, a spiral tube, an adapter, a Y-shaped adapter, a concentrated solution outlet microtube and a waste liquid outlet microtube; the spiral tube is connected to the lower edge of the rubber layer Fixed connection, the spiral tube and the rubber layer are evenly spirally wound on the periphery of the round rod; the top end of the spiral tube is the inlet end, and the bottom end is the outlet end; the inlet end is connected to the adapter, and the outlet end is connected to the Y-shaped adapter; The Y-shaped adapter is a one-in-two-out structure, which includes a liquid inlet, a concentrated liquid outlet, a waste liquid outlet, a first connected joint, a second connected joint and a third connected joint, the concentrated liquid outlet and the The waste liquid outlets communicate with the liquid inlet respectively; the liquid inlet communicates with the outlet port through the first connected joint; the concentrated liquid outlet micropipe communicates with the concentrated liquid outlet through the second connected joint; The three-piece connector communicates with the waste liquid outlet.
上述螺旋圆管是螺旋上升的结构，螺旋方向可以是左旋也可以是右旋；螺旋圆管可以是透明材料也可以是半透明或者不透明材料；螺旋圆管可以是柔性材料也可以是刚性材料。The above-mentioned spiral tube is a spiral structure, and the spiral direction can be left-handed or right-handed; the spiral tube can be made of transparent material or translucent or opaque material; the spiral tube can be made of flexible material or rigid material.
工作原理：如图1、图5和图6所示，本发明低成本微米粒子浓缩装置浓缩样品时，用注射器吸入粒子悬浮液，以特定的流速将粒子悬浮液导入螺旋圆管中；粒子悬浮液中的微米粒子将受到流体拖曳力的作用而沿着流动方向运动；由于流道中伯啸叶流的抛物线形速度剖面，微米粒子将受到指向壁面的剪切诱导惯性升力；当微米粒子靠近壁面时，由于微米粒子因为自转而旋转产生的对称尾迹被壁面影响而产生一个指向圆管中心线的壁面诱导惯性升力；当流体流经弯流道时在垂直主流动方向上产生了两个旋转方向相反的涡，被称作为Dean流，由于Dean流的引入而产生了Dean拽力；弯流道中粒子不仅受到惯性迁移效应影响而迁移，还受到由弯曲率诱导产生的截面二次流引起的Dean拽力影响；因此，可近似认为微米粒子在Dean拽力和惯性升力的共同耦合作用下迁移至靠近螺旋圆管内壁的一侧；最终绝大部分微米粒子通过Y型转接头的浓缩液出口从浓缩液出口微管流出并被收集，而剩余悬浮液则为废液，通过Y型转接头的废液出口从废液出口微管流出，最终收集，从而实现了粒子悬浮液的浓缩。Working principle: as shown in Figure 1, Figure 5 and Figure 6, when the low-cost micron particle concentration device of the present invention concentrates samples, the particle suspension is inhaled with a syringe, and the particle suspension is introduced into the spiral circular tube at a specific flow rate; the particle suspension The microparticles in the liquid will be affected by the drag force of the fluid and move along the flow direction; due to the parabolic velocity profile of the Bo Xiaoye flow in the flow channel, the microparticles will be subjected to the shear-induced inertial lift pointing to the wall; when the microparticles are close to the wall When , the symmetrical wake generated by the rotation of the microparticles due to the rotation is affected by the wall to generate a wall-induced inertial lift pointing to the centerline of the circular tube; when the fluid flows through the curved channel, two rotation directions are generated in the vertical main flow direction The opposite vortex, called Dean flow, produces Dean drag due to the introduction of Dean flow; particles in the curved flow channel are not only migrated by the effect of inertial migration, but also by the Dean force caused by the secondary flow of the section induced by the curvature. Therefore, it can be approximated that the microparticles migrate to the side close to the inner wall of the spiral tube under the joint coupling of Dean’s drag force and inertial lift force; finally, most of the microparticles pass through the concentrated solution outlet of the Y-shaped adapter from the The concentrated liquid outlet microtube flows out and is collected, while the remaining suspension is waste liquid, which flows out from the waste liquid outlet microtube through the waste liquid outlet of the Y-shaped adapter and is finally collected, thereby realizing the concentration of the particle suspension.
上述Dean拽力为：当流体流经弯流道时在垂直主流动方向上产生了两个旋转方向相反的涡，被称作为Dean流，由于Dean流的引入而产生了Dean拽力。The above-mentioned Dean drag force is: when the fluid flows through the curved channel, two vortices with opposite rotation directions are generated in the vertical main flow direction, which are called Dean flow, and the Dean drag force is generated due to the introduction of the Dean flow.
所述进口端与接合器为密封连接，能防止悬浮液渗漏。The inlet port is in a sealed connection with the adapter, which can prevent the suspension from leaking.
所述第一连体接头、第二连体接头和第三连体接头均为密封连接头，能进一步防止悬浮液渗漏。The first connected joint, the second connected joint and the third connected joint are all sealed joints, which can further prevent the suspension from leaking.
优选：所述螺旋圆管螺旋节距与橡胶层的宽度和螺旋直径尺寸关系为：Preferably: the relationship between the helical pitch of the helical circular tube and the width and helical diameter of the rubber layer is:
其中，γ为螺旋圆管升角，h为橡胶层的宽，p为螺旋圆管节距，d为螺旋直径。Among them, γ is the pitch angle of the spiral tube, h is the width of the rubber layer, p is the pitch of the spiral tube, and d is the diameter of the spiral.
优选：所述螺旋圆管内径尺寸和浓缩粒子的尺寸关系为：Preferably: the relationship between the inner diameter of the spiral circular tube and the size of the concentrated particles is:
， ,
其中，为浓缩粒子的最大直径，为螺旋圆管内径尺寸。in, is the maximum diameter of the concentrated particle, is the inner diameter of the spiral tube.
上述低成本微米粒子浓缩装置的制作方法，包括以下几个步骤：The manufacturing method of the above-mentioned low-cost micron particle concentration device includes the following steps:
1）、制作一个狭窄的橡胶层，放置在水平桌面上；1) Make a narrow rubber layer and place it on a horizontal table;
2）、将螺旋圆管拉直，通过粘合剂使圆管和橡胶层下边缘固联；2) Straighten the spiral tube, and connect the tube and the lower edge of the rubber layer with an adhesive;
3）、将螺旋圆管和橡胶层的结合体紧密地旋绕在圆棒上；3) Tightly wind the combination of the spiral tube and the rubber layer on the round rod;
4）、螺旋圆管顶端的进口端通过接合器与注射器连通，出口端通过第一连体接头与Y型转接头进液口连接；4) The inlet end of the top of the spiral tube is connected to the syringe through an adapter, and the outlet end is connected to the liquid inlet of the Y-shaped adapter through the first connected joint;
5）、Y型转接头的浓缩液出口和废液出口分别通过第二连体接头、第三连体接头连接浓缩液出口微管和废液出口微管实现粒子浓缩。5) The concentrated liquid outlet and the waste liquid outlet of the Y-shaped adapter are respectively connected to the concentrated liquid outlet microtube and the waste liquid outlet microtube through the second connected joint and the third connected joint to realize particle concentration.
优选：所述步骤3）中螺旋圆管的螺旋节距与橡胶层的宽度和螺旋直径有关，旋绕总圈数与橡胶层长度关系为：Preferably: the helical pitch of the helical circular tube in step 3) is related to the width and helical diameter of the rubber layer, and the relationship between the total number of turns and the length of the rubber layer is:
其中，n为旋绕圈数，L为橡胶层长度，d为螺旋圆管螺旋直径。Among them, n is the number of turns, L is the length of the rubber layer, and d is the helical diameter of the helical tube.
优选：所述步骤4）中接合器与注射器为密封连接。Preferably: the adapter and the syringe in the step 4) are in airtight connection.
本发明未提及的技术均为现有技术。The technologies not mentioned in the present invention are all prior art.
有益效果：本发明的螺旋圆管结构通过适当调整样品的流速，突破传统微流控中低雷诺数的观念，巧妙利用微流体惯性效应来实现微米级生物粒子的高通量、连续流尺寸浓缩；本发明结构简单、通量高；仅需控制螺旋圆管流道的内径以及螺旋直径、螺距和总圈数，无需借助外场；同时，相比较其他制作微通道复杂的工艺方法，本发明直接使用毛细微管旋绕成螺旋微通道结构，制作成本大大降低，工作效率显著提高。Beneficial effects: the spiral circular tube structure of the present invention breaks through the concept of low Reynolds number in traditional microfluidics by properly adjusting the flow rate of the sample, and cleverly utilizes the microfluidic inertial effect to realize high-throughput and continuous flow size concentration of micron-sized biological particles The present invention has simple structure and high throughput; it only needs to control the inner diameter of the helical circular tube flow channel, the helical diameter, the pitch and the total number of turns, without the need for an external field; at the same time, compared with other complicated process methods for making microchannels, the present invention directly The capillary microtube is wound into a helical microchannel structure, the manufacturing cost is greatly reduced, and the working efficiency is significantly improved.
附图说明Description of drawings
图1是本发明的结构示意图；Fig. 1 is a structural representation of the present invention;
图2是Y型转接头结构分解示意图；Fig. 2 is a schematic exploded view of the structure of the Y-shaped adapter;
图3是螺旋圆管与橡胶层连接示意图；Fig. 3 is a schematic diagram of the connection between the spiral tube and the rubber layer;
图4是螺旋圆管旋绕方法示意图；Fig. 4 is a schematic diagram of a spiral circular tube winding method;
图5是本发明微流道粒子浓缩原理示意图；Fig. 5 is a schematic diagram of the microchannel particle concentration principle of the present invention;
图6是螺旋圆管出口端截面粒子分布示意图；Fig. 6 is a schematic diagram of particle distribution at the outlet end section of the spiral circular tube;
图中，1为注射器、2为粒子悬浮液、3为圆棒、4为橡胶层、5为螺旋圆管、6为Y型转接头、7为进口端、8为接合器、9为出口端、10为浓缩液出口微管、11为废液出口微管、12为第二连体接头、13为第三连体接头、14为浓缩液出口、15为废液出口、16为进液口、17为第一连体接头、19为废液、20为微米粒子、22为伯啸叶流的抛物线形速度、24为Dean流、26为橡胶层旋转方向。F1为流体拖曳力、F2为剪切诱导惯性升力、F3为壁面诱导惯性升力、F4为Dean拽力。In the figure, 1 is a syringe, 2 is a particle suspension, 3 is a round rod, 4 is a rubber layer, 5 is a spiral tube, 6 is a Y-shaped adapter, 7 is an inlet port, 8 is an adapter, and 9 is an outlet port , 10 is the concentrated liquid outlet micropipe, 11 is the waste liquid outlet micropipe, 12 is the second connecting joint, 13 is the third connecting joint, 14 is the concentrated liquid outlet, 15 is the waste liquid outlet, 16 is the liquid inlet , 17 is the first connecting joint, 19 is the waste liquid, 20 is the micron particle, 22 is the parabolic velocity of the Bo Xiao leaf flow, 24 is the Dean flow, 26 is the rotation direction of the rubber layer. F1 is fluid drag force, F2 is shear-induced inertial lift force, F3 is wall-induced inertial lift force, and F4 is Dean drag force.
具体实施方式detailed description
为了更好地理解本发明，下面结合实施例进一步阐明本发明的内容，但本发明的内容不仅仅局限于下面的实施例。In order to better understand the present invention, the content of the present invention is further illustrated below in conjunction with the examples, but the content of the present invention is not limited to the following examples.
实施例1Example 1
如图1-6所示，一种低成本微米粒子浓缩装置，包括圆棒3、橡胶层4、螺旋圆管5、接合器8、Y型转接头6、浓缩液出口微管10和废液出口微管11；所述螺旋圆管5与橡胶层4下边缘固连，螺旋圆管5和橡胶层4均匀地螺旋缠绕在圆棒3外围；所述螺旋圆管5顶端为进口端7，底端为出口端9；进口端7与接合器8连接，出口端9与Y型转接头6连接；所述Y型转接头6为一进二出式结构，内部包括进液口16、浓缩液出口14、废液出口15、第一连体接头17、第二连体接头12和第三连体接头13，浓缩液出口14和废液出口15分别与进液口16相通；所述进液口16通过第一连体接头17与出口端9相通；浓缩液出口微管10通过第二连体接头12与浓缩液出口14相通；废液出口微管11通过第三连体接头13与废液出口15相通；所述进口端7与接合器8为密封连接；所述第一连体接17头、第二连体接头12和第三连体接头13均为密封连接头；所述螺旋圆管5螺旋节距与橡胶层4的宽度和螺旋直径尺寸关系为：As shown in Figures 1-6, a low-cost micron particle concentration device includes a round rod 3, a rubber layer 4, a spiral tube 5, an adapter 8, a Y-shaped adapter 6, a concentrated solution outlet microtube 10 and waste liquid Outlet microtube 11; the spiral tube 5 is fixedly connected to the lower edge of the rubber layer 4, and the spiral tube 5 and the rubber layer 4 are evenly wound around the periphery of the round rod 3; the top of the spiral tube 5 is the inlet end 7, The bottom end is the outlet port 9; the inlet port 7 is connected to the adapter 8, and the outlet port 9 is connected to the Y-shaped adapter 6; the Y-shaped adapter 6 is a one-in-two-out structure, which includes a liquid inlet 16, a concentration The liquid outlet 14, the waste liquid outlet 15, the first connected joint 17, the second connected joint 12 and the third connected joint 13, the concentrated liquid outlet 14 and the waste liquid outlet 15 communicate with the liquid inlet 16 respectively; The liquid port 16 communicates with the outlet port 9 through the first connected joint 17; the concentrated liquid outlet micropipe 10 communicates with the concentrated liquid outlet 14 through the second connected joint 12; The waste liquid outlet 15 is communicated; the inlet port 7 is in a sealed connection with the adapter 8; the first joint joint 17, the second joint joint 12 and the third joint joint 13 are all sealed joints; the The relationship between the helical pitch of the helical tube 5 and the width and helical diameter of the rubber layer 4 is:
其中，γ为螺旋圆管升角，h为橡胶层的宽度，p为螺旋圆管节距，d为螺旋直径；螺旋圆管5内径尺寸和浓缩粒子的尺寸关系为：Wherein, γ is the angle of rise of the spiral circular tube, h is the width of the rubber layer, p is the pitch of the spiral circular tube, and d is the diameter of the spiral; the relationship between the inner diameter of the spiral circular tube 5 and the size of the concentrated particles is:
， ,
其中，为浓缩粒子的最大直径，为螺旋圆管5内径尺寸。in, is the maximum diameter of the concentrated particle, It is the internal diameter size of the spiral tube 5.
螺旋圆管5是螺旋上升的结构，螺旋方向为左旋；螺旋圆管5为刚性透明材料。The spiral tube 5 is a spiral structure, and the spiral direction is left-handed; the spiral tube 5 is a rigid transparent material.
一种低成本微米粒子浓缩装置的制作方法，包括以下几个步骤：A method for manufacturing a low-cost micron particle concentration device, comprising the following steps:
1）、制作一个狭窄的橡胶层4，放置在水平桌面上；1) Make a narrow rubber layer 4 and place it on a horizontal table;
2）、将螺旋圆管5拉直，通过粘合剂使圆管和橡胶层4下边缘固联；2) Straighten the spiral tube 5, and connect the tube and the lower edge of the rubber layer 4 with an adhesive;
3）、将螺旋圆管5和橡胶层4的结合体紧密地旋绕在圆棒3上；3) Tightly wind the combination of the spiral tube 5 and the rubber layer 4 on the round bar 3;
4）、螺旋圆管5顶端的进口端7通过接合器8与注射器1连通，出口端9通过第一连体接头17与Y型转接头6进液口16连接；4) The inlet end 7 at the top of the spiral tube 5 communicates with the syringe 1 through the adapter 8, and the outlet end 9 connects with the liquid inlet 16 of the Y-shaped adapter 6 through the first connecting joint 17;
5）、Y型转接头6的浓缩液出口14和废液出口15分别通过第二连体接头12、第三连体接头13连接浓缩液出口微管和废液出口微管11实现粒子浓缩。5) The concentrated liquid outlet 14 and the waste liquid outlet 15 of the Y-shaped adapter 6 are respectively connected to the concentrated liquid outlet microtube and the waste liquid outlet microtube 11 through the second connecting joint 12 and the third connecting joint 13 to realize particle concentration.
所述步骤3）中螺旋圆管5的螺旋节距与橡胶层4的宽度和螺旋直径有关，旋绕总圈数与橡胶层4的长度关系为：The helical pitch of the helical tube 5 in step 3) is related to the width and helical diameter of the rubber layer 4, and the relationship between the total number of turns and the length of the rubber layer 4 is:
其中，n为旋绕圈数，L为橡胶层4长度，d为螺旋圆管5螺旋直径。Wherein, n is the number of turns, L is the length of the rubber layer 4, and d is the helical diameter of the helical tube 5.
所述步骤4）中接合器8与注射器1为密封连接。In the step 4), the adapter 8 is in a sealed connection with the syringe 1 .
如图1、图5和图6所示，本发明低成本微米粒子20浓缩装置浓缩样品时，用注射器1吸入粒子悬浮液2，以特定的流速将粒子悬浮液2导入螺旋圆管5中；粒子悬浮液2中的微米粒子20将受到流体拖曳力F1的作用而沿着流动方向运动；由于流道中伯啸叶流的抛物线形速度22剖面，微米粒子20将受到指向壁面的剪切诱导惯性升力F3；当微米粒子20靠近壁面时，由于微米粒子20因为自转而旋转产生的对称尾迹被壁面影响而产生一个指向圆管中心线的壁面诱导惯性升力F2；当流体流经弯流道时在垂直主流动方向上产生了两个旋转方向相反的涡，被称作为dean流24，由于dean流24的引入而产生了dean拽力F4；弯流道中粒子不仅受到惯性迁移效应影响而迁移，还受到由弯曲率诱导产生的截面二次流引起的Dean拽力F4影响；因此，可近似认为微米粒子20在Dean拽力F4和惯性升力的共同耦合作用下迁移至靠近螺旋圆管5内壁的一侧；最终绝大部分微米粒子20通过Y型转接头6的浓缩液出口14从浓缩液出口微管10流出并被收集，而剩余悬浮液2则为废液19，通过Y型转接头6的废液出口15从废液出口微管11流出，最终收集，从而实现了粒子悬浮液2的浓缩。As shown in Figure 1, Figure 5 and Figure 6, when the low-cost micron particle 20 concentration device of the present invention concentrates the sample, the particle suspension 2 is inhaled with the syringe 1, and the particle suspension 2 is introduced into the spiral circular tube 5 at a specific flow rate; The microparticles 20 in the particle suspension 2 will be affected by the fluid drag force F1 and move along the flow direction; due to the parabolic velocity 22 profile of the primary whistle flow in the flow channel, the microparticles 20 will be subjected to shear-induced inertia pointing to the wall Lift F3; when the microparticle 20 is close to the wall, the symmetrical wake generated by the rotation of the microparticle 20 is affected by the wall to generate a wall-induced inertial lift F2 pointing to the centerline of the circular tube; when the fluid flows through the curved channel Two vortices with opposite rotation directions are generated in the direction perpendicular to the main flow, which are called dean flow 24. The introduction of dean flow 24 produces a dean drag force F4; It is affected by the Dean drag force F4 caused by the secondary flow induced by the bending rate; therefore, it can be approximately considered that the microparticles 20 migrate to a section close to the inner wall of the spiral tube 5 under the combined action of the Dean drag force F4 and the inertial lift force. side; finally most of the micron particles 20 flow out from the concentrate outlet micropipe 10 through the concentrate outlet 14 of the Y-type adapter 6 and are collected, while the remaining suspension 2 is waste liquid 19, which is passed through the outlet of the Y-type adapter 6 The waste liquid outlet 15 flows out from the waste liquid outlet micropipe 11 and is finally collected, thereby realizing the concentration of the particle suspension 2 .
本发明的螺旋圆管5结构通过适当调整样品的流速，突破传统微流控中低雷诺数的观念，巧妙利用微流体惯性效应来实现微米级生物粒子的高通量、连续流尺寸浓缩；本发明结构简单、通量高；仅需控制螺旋圆管5流道的内径以及螺旋直径、螺距和总圈数，无需借助外场；同时，相比较其他制作微通道复杂的工艺方法，本发明直接使用毛细微管旋绕成螺旋微通道结构，制作成本大大降低，工作效率显著提高。The structure of the spiral tube 5 of the present invention breaks through the concept of low Reynolds number in traditional microfluidics by properly adjusting the flow rate of the sample, and cleverly utilizes the microfluidic inertial effect to achieve high flux and continuous flow size concentration of micron-sized biological particles; The invention has simple structure and high throughput; it only needs to control the inner diameter of the flow channel of the spiral tube 5, the helical diameter, the pitch and the total number of turns, without the need for an external field; at the same time, compared with other complicated process methods for making microchannels, the present invention directly uses The capillary microtube is wound into a helical microchannel structure, the manufacturing cost is greatly reduced, and the working efficiency is significantly improved.
实施例2Example 2
与实施例1基本相同，所不同的是：螺旋圆管5的螺旋方向为右旋；螺旋圆管5柔性不透明材料。It is basically the same as the embodiment 1, except that: the helical direction of the spiral tube 5 is right-handed; the spiral tube 5 is made of flexible opaque material.
实施例3Example 3
与实施例1基本相同，所不同的是：螺旋圆管5的螺旋方向为右旋；螺旋圆管5柔性半透明材料。It is basically the same as Embodiment 1, except that: the spiral direction of the spiral tube 5 is right-handed; the spiral tube 5 is made of flexible translucent material.
以上仅是本发明的优选实施方式，应当指出：对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以对各设施位置进行调整，这些调整也应视为本发明的保护范围。The above are only preferred embodiments of the present invention, and it should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, the positions of each facility can also be adjusted, and these adjustments should also be regarded as the present invention. protection scope of the invention.
| Application Number | Priority Date | Filing Date | Title |
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| CN201510380399.5ACN104923323B (en) | 2015-07-02 | 2015-07-02 | A kind of low cost micron particle enrichment facility and preparation method thereof |
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| CN201510380399.5ACN104923323B (en) | 2015-07-02 | 2015-07-02 | A kind of low cost micron particle enrichment facility and preparation method thereof |
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| CN201510380399.5AActiveCN104923323B (en) | 2015-07-02 | 2015-07-02 | A kind of low cost micron particle enrichment facility and preparation method thereof |
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