A kind of many micropores plant amylum coupling oligochitosan styptic powder and preparation methodTechnical field
A kind of many micropores plant amylum coupling oligochitosan styptic powder and preparation method, this invention relates to biomedicine field, and being a kind ofly provides the technology that quick-acting haemostatic powder, cost are low, swelling ratio is high.
Background technology
Data shows, in modern war, even if having absolute technical advantage and fast after send the army of condition, still have the wounded of 20% before not accepting definitive treatment, die from battlefield one line, its main cause is severe loss of blood, even if transfer to rear medical institutions, the hemorrhage main cause being still death and complication generation.Therefore, the novel material of making a study of subjects of the applicable first aid of development just becomes the direction that military medicine scientific research department of various countries first develops.Along with the fast development of materialogy, first aid and surgical hemostasis investigation of materials have had rapid progress, but conventional hemostatic material still exists many deficiencies at present.
At present conventional hemostasis treatment material category is various, body surface point, the hemorrhage hemostatic material of planar, main rely on the chemical factors of material own to promote blood coagulation reaches localized plug or pressure dressing occluding vascular thus reaches hemostasis object.In China's emergency care of trauma, conventional body surface hemostatic material has first-aid kit, tourniquet etc.; The temperature sensing material that deep hemostasis material mainly contains the solid material such as microcoils, gelfoam and recently occurs.
Quick-acting haemostatic powder and functional hemostasis are the directions of following hemostatic material development.Develop various new wound hemostatic material at present, as: oligochitosan, microporous polysaccharide hemospheres, oxidized cellulose, olefin(e) acid esters tissue glue etc.
Oligochitosan is a kind of alkaline oligosaccharide being present in occurring in nature, is only made up of about 20 monosaccharide molecule, is a kind of elementary derivant of chitin deacetylase base.The main hemostatic mechanism of oligochitosan is that part positively charged in the molecule composition electronegative with erythrocyte, leukocyte and platelet etc. in blood combines, form Thromboembolus and then produce Blood clotting, and material such as cellular matrix secretion hyaluronic acid, cell growth factor etc. can be made to increase, promote wound healing.The Blood clotting of oligochitosan does not rely on thrombin and platelet, therefore can be applied in coagulation disorders patient.In addition, oligochitosan also has good antibacterial action, can suppress the growth of wound surface common bacteria and fungus.Oligochitosan Wound dressing has good water suction, breathability, compares traditional Wound dressing, and wound tissue can obtain enough partial pressure of oxygens, and then is conducive to growth and the reparation of epithelial tissue, can be suppressed to fibrocellular hyper-proliferative simultaneously, alleviates cicatrix reaction.At present, the sthptic sponge of oligochitosan coating, hemostatic gauze have been applied in first aid hemostasis and surgical operation, wherein add hemorrhage and can strengthen haemostatic effect.
Along with the development of modern technologies, no matter be complicated war wound or daily life, the incidence rate of damage improves just year by year, and this has higher requirement to first aid hemostatic treatment, and the material that development has better anthemorrhagic performance is imperative.As the material of bleeding-stopping dressing or hemorrhage except need possessing good anthemorrhagic performance, also need to possess excellent biocompatibility, the features such as toxic and side effects is little, easy to use.Meanwhile, along with the progress of materialogy, to original hemostatic material carry out physics, chemical property reprocessing with improve just become a kind of practical way.
Summary of the invention
The invention provides a kind of many micropores plant amylum coupling oligochitosan styptic powder and preparation method, this styptic powder is Biodegradable material, and be a kind of polysaccharide microsphere, main component is oligochitosan, calcium chloride and plant amylum.
Oligochitosan and plant amylum are the biological polysaccharides that can play two kinds of different clotting mechanisms respectively, possess good histocompatibility simultaneously.Both organic assembling are added simultaneously calcium chloride, liquid paraffin, Tween 80 be prepared into have many wrinkled surfaces, can the microsphere of water absorption and swelling fast, more excellent haemostatic effect can be played.
The oligochitosan styptic powder of the present invention is combined be prepared from by oligochitosan, calcium chloride, plant amylum, liquid paraffin, Tween 80, and wherein calcium chloride is cross-linking agent, and Tween 80 is emulsifying agent.
A kind of many micropores plant amylum coupling oligochitosan styptic powder and each composition proportion of preparation method are:
Oligochitosan 0.4 ~ 1.0g
Calcium chloride 0.4 ~ 0.8g
Plant amylum 0.4 ~ 1.0g
Liquid paraffin 125 ~ 500mL
Tween 80 8 ~ 38mL
Preparation method:
1. obtained aqueous solution: according to component proportion oligochitosan and plant amylum be dissolved in 50 ~ 100mL distilled water respectively and be mixed with aqueous solution, get both equivalent solution, be fully uniformly mixed, make aqueous solution;
2. prepare oil-phase solution: by proportioning by liquid paraffin and Tween 80 mixing, make oil-phase solution;
3. prepare calcium chloride solution: be dissolved in by calcium chloride in 12 ~ 25mL distilled water, stir, be mixed with calcium chloride solution;
4. prepare hydrogel microsphere: aqueous phase solution is added oil-phase solution, rapid stirring emulsifying, then add calcium chloride solution and fully stir, be cross-linked to form hydrogel microsphere, staticly settle, abandon supernatant;
5. washing dehydration: by precipitation petroleum ether, to remove liquid paraffin and Tween 80, then carries out serial dehydration with ethanol, final drying, makes powder.
With the particle diameter of particle size determination systematic survey oligochitosan styptic powder, its particle size range is between 10 ~ 100 μm.Scanning electron microscope shows, and granule is chondritic, and surface is covered with fold.Touch your fluid environment with wound fluid, granules swell rate reaches 300%, and in rabbit spleen model, haemostatic effect is obviously better than YUNNAN BAIYAO.
Detailed description of the invention
Now in conjunction with the embodiments, the present invention is elaborated:
Embodiment:
1. 1g oligochitosan and 1g plant amylum are dissolved in 100ml distilled water respectively;
2. oligochitosan solution is added plant amylum solution, be fully uniformly mixed;
3. above-mentioned mixed liquor is added rapid stirring 30min in the emulsion of 500ml liquid paraffin and 37.5ml Tween 80 composition, mixing speed is 1000 revs/min;
4. in above-mentioned solution, add 40ml2% calcium chloride solution, fully stir, be cross-linked to form hydrogel microsphere, staticly settle, discard supernatant solution;
5. use 100ml petroleum ether 3 times respectively, then become powder with 35%, 70%, 100% graded ethanol solutions dehydration, vacuum drying respectively.
Styptic powder of the present invention, preparation method is simple and convenient, low production cost, and product water absorption and swelling rate is high, easy to use, and haemostatic effect is excellent.