CN104911105A - Chrysophyte medium - Google Patents

Abstract

The invention discloses an isochrysis galbana parke medium, including the following components by mass: NaNO3 with the mass concentration of 60-120 mg/L, phosphate (NaH2PO4, KH2PO4) with the mass concentration of 8-12 mg/L, ferric ammonium citrate with the mass concentration of 2.5-3.5 mg/L, Na2EDTA with the mass concentration of 10-12 mg/L, naphthalene acetamide with the mass concentration of 1 mg/L and polydimethylsiloxane with the mass concentration of 1 ml/L. When the isochrysis galbana parke medium is used for culture of gymnodinium sanguineum, the gymnodinium sanguineum has the advantage of fast growth rate and large cell density, and the isochrysis galbana parke medium is is suitable for large-scale mass production during culture of. When the isochrysis galbana parke medium is used for culture of chrysophyceae, the chrysophyceae has the advantage of fast growth rate and large cell density.

Description

A kind of substratum for chrysophyceae

Technical field

The present invention relates to algae media technical field, particularly relate to the substratum of a kind of chrysophyceae.

Background technology

Chrysophyceae, is mostly the unicellular algae that swims of size 2-7 micron.Because of its acellular wall, be rich in unsaturated fatty acids and ecological suitability is strong, long speed is fast, is suitable for large-scale breeding, is considered to the desirable bait of marine animal artificial breeding, as the high-quality starter diet of the famous and precious fishery products such as mussel, oyster, mud blood clam, prawn.Now need a kind of substratum, can promote that Isochrysis galbana grows fast.Naphthalene acetamide is a kind of plant growth regulating substance, has very good effect to promotion cell fission.Polydimethylsiloxane is a kind of well sequestrant, can promote that frustule is to the absorption of nutritive element.

Summary of the invention

Based on the technical problem that background technology exists, the present invention proposes a kind of ball chrysophyceae substratum, when cultivating chrysophyceae, Isochrysis galbana is had growth velocity is fast, cell density is large feature.

For solving the problems of the technologies described above, a kind of ball chrysophyceae substratum comprises the component of following mass concentration: NaNO₃60-120mg/L, phosphoric acid salt (NaH₂pO₄, KH₂pO₄) 8-12mg/L, ferric ammonium citrate 2.5-3.5mg/L and Na₂eDTA 10-12mg/L.

As preferred technical scheme, a kind of ball chrysophyceae substratum comprises the component of following mass concentration: NaNO₃60-120mg/L, phosphoric acid salt (NaH₂pO₄, KH₂pO₄) 8-12mg/L, ferric ammonium citrate 2.5-3.5mg/L, Na₂eDTA 10-12mg/L, naphthalene acetamide 0.1-1mg/L and polydimethylsiloxane 1ml/L.

Advantage of the present invention is: substratum of the present invention, when cultivating chrysophyceae, has chrysophyceae is had growth velocity is fast, cell density is large feature.

Embodiment

Below in conjunction with specific embodiment, the substratum of chrysophyceae provided by the invention is described in detail.

Embodiment 1

A kind of Isochrysis galbana substratum that the present invention proposes, comprises following component: NaNO₃120mg/L, KH₂pO₄12mg/L, ferric ammonium citrate 3.5mg/L, Na₂eDTA 12mg/L, naphthalene acetamide 0.1mg/L and polydimethylsiloxane 1ml/L.

Embodiment 2

A kind of Isochrysis galbana substratum that the present invention proposes, comprises following component: NaNO₃60mg/L, KH₂pO₄8mg/L, ferric ammonium citrate 2.5mg/L, Na₂eDTA 10mg/L, naphthalene acetamide 1mg/L and polydimethylsiloxane 1ml/L.

With the substratum of embodiment 1 and embodiment 2 gained for experimental group, with seawater F/2 substratum as a control group.The Isochrysis galbana 3011 of taking the logarithm vegetative period is inoculated in this substratum to be cultivated, illumination every day 10h, and intensity of illumination is about 50 μm of ol/ (m²s), training case temperature controls at 25 ± 1 DEG C, records frustule concentration in substratum at set intervals.Experimental result is as shown in the table:

Experimentally result is carried out drawing and is obtained table 1.

	0th day	3rd day	5th day	7th day	9th day
						Embodiment 1	180	1360	1500	1730	1800
Embodiment 2	180	1380	1580	1770	1800
						Seawater f/2 substratum	180	1080	1202	1400	1500

As can be seen from this table, use substratum provided by the present invention than using contrast substratum that Isochrysis galbana 3011 can be made to breed more quickly, the time reaching density peak is faster 2 days than use control group, and the maximum concentration that Isochrysis galbana can reach is also higher than the seawater F/2 substratum about 20% of control group, the substratum describing confession of the present invention has clear superiority.

The above; be only the present invention's preferably embodiment; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.

Claims (2)

1. an Isochrysis galbana substratum, is characterized in that it comprises the component of following mass concentration: NaNO₃60-120mg/L, phosphoric acid salt (NaH₂pO₄, KH₂pO₄) 8-12mg/L, ferric ammonium citrate 2.5-3.5mg/L and Na₂eDTA10-12mg/L.

2. chrysophyceae substratum according to claim 1, is characterized in that it comprises the component of following mass concentration: NaNO₃60-120mg/L, phosphoric acid salt (NaH₂pO₄, KH₂pO₄) 8-12mg/L, ferric ammonium citrate 2.5-3.5mg/L, Na₂eDTA10-12mg/L, naphthalene acetamide 1mg/L and polydimethylsiloxane 1ml/L.