技术领域technical field
本发明涉及一种快速、灵敏、便捷的水族箱水质快速检测的6合1试纸，包括亚硝酸盐、硝酸盐、总硬度、总氯、总碱、pH值6项指标，属于水质安全监测领域。The invention relates to a fast, sensitive and convenient 6-in-1 test paper for rapid detection of aquarium water quality, including 6 indicators of nitrite, nitrate, total hardness, total chlorine, total alkali and pH value, belonging to the field of water quality safety monitoring .
背景技术Background technique
养鱼必先养水，在饲养水族生物的过程中，及时监测水族箱水质是否稳定、健康是非常必要的。水族箱中的生物最佳的生存环境就是自然界中该物种的生存和繁衍区域的生态环境，如何在水族箱中营造该物种的栖息地是水族饲养者所关注的主要问题，目前，水族生物饲养者一般以亚硝酸盐、硝酸盐、总硬度、总氯、总碱、pH值等水质测量数据为依据同养殖物种原产地的测量数据进行比较，最大可能地调整水族箱中的各种数值与养殖物种繁衍地接近甚至等同。To raise fish, you must first raise water. In the process of raising aquatic organisms, it is very necessary to monitor whether the water quality of the aquarium is stable and healthy in time. The best living environment for the organisms in the aquarium is the ecological environment of the survival and reproduction area of the species in nature. How to create the habitat of the species in the aquarium is the main concern of the aquarium breeders. At present, the aquatic organisms are raised Generally, the water quality measurement data such as nitrite, nitrate, total hardness, total chlorine, total alkali, and pH value are compared with the measurement data of the origin of the cultured species, and the various values in the aquarium are adjusted to the greatest possible extent. Farmed species reproduce close to or even identically.
水族箱水质的各项指标都与水族生物的健康息息相关：All indicators of aquarium water quality are closely related to the health of aquatic organisms:
(1)亚硝酸盐来源于亚硝化细菌对于氨的氧化作用，二氧化氮是血液毒，毒性反应为是血红素产生异变丧失其携氧功能，危害水族生物。国家标准中Ⅰ类水质的亚硝酸盐含量应小于0.001mg/L、硝酸盐含量应小于2.0mg/L。(1) Nitrite comes from the oxidation of ammonia by nitrosifying bacteria. Nitrogen dioxide is a blood poison. The toxic reaction is that the hemoglobin mutates and loses its oxygen-carrying function, endangering aquatic organisms. According to national standards, the nitrite content of Class I water quality should be less than 0.001mg/L, and the nitrate content should be less than 2.0mg/L.
(2)水的总硬度是指水中溶解的多价金属离子的含量，其中以钙、镁离子的含量占绝大多数，因此硬度又有钙硬度及镁硬度之分，二者之和称为总硬度。国际上以钙的碳酸盐(CaCO3)含量来描述GH值，单位为：mg/L。国家标准中Ⅰ类水质的总硬度应小于150mg/L。(2) The total hardness of water refers to the content of multivalent metal ions dissolved in water, among which the content of calcium and magnesium ions accounts for the vast majority, so the hardness is divided into calcium hardness and magnesium hardness. The sum of the two is called total hardness. Internationally, the GH value is described by the content of calcium carbonate (CaCO3 ), and the unit is mg/L. The total hardness of Class I water quality in the national standard should be less than 150mg/L.
(3)水族箱养殖用水是多数为自来水，自来水生产过程中消毒采用氯化法，主要作用物质是氯气。自来水中氯气浓度过高超过会腐蚀鱼体粘膜，浓度超过0.2mg/L会使绝大多数鱼类产生急性中毒。因此必须严格控制水族箱中的氯气。国家Ⅰ类水质标准中氯气含量应低于0.1mg/L。(3) Most of the water used for aquarium cultivation is tap water. Chlorination is used for disinfection in the production process of tap water, and the main active substance is chlorine gas. If the concentration of chlorine in tap water is too high, it will corrode the mucous membrane of fish, and if the concentration exceeds 0.2mg/L, it will cause acute poisoning to most fish. Therefore, the chlorine in the aquarium must be strictly controlled. The chlorine content in the national Class I water quality standard should be less than 0.1mg/L.
(4)碱度是指水中所含碳酸氢根等弱酸离子的量，本来淡水中最多的盐是碳酸盐类，它包括碳酸氢盐和碳酸盐，由于碳酸盐在水中溶解度低，因此水中主要是碳酸氢盐。它可以中和水质中增加或减少的游离氢离子，抑制氢离子的波动，以维持恒定的pH值，因此碱度的控制在水族箱水质管理中不不可或缺。鱼类在过高碱度的水中，体表分泌大量黏液，鳃出血，迅速死亡。(4) Alkalinity refers to the amount of weak acid ions such as bicarbonate in water. Originally, the most salt in fresh water is carbonate, which includes bicarbonate and carbonate. Because carbonate has low solubility in water, it is The water is mainly bicarbonate. It can neutralize the increase or decrease of free hydrogen ions in water quality, suppress the fluctuation of hydrogen ions to maintain a constant pH value, so the control of alkalinity is indispensable in the management of aquarium water quality. Fish in water with too high alkalinity will secrete a large amount of mucus on the body surface, bleed from the gills, and die quickly.
(5)水的pH值主要取决于水中溶解的二氧化碳和碳酸盐的比例。一般来说，二氧化碳含量越多，水越偏向酸性；反之，则偏向碱性。水中的酸碱度，对鱼、蚌及其他水生生物的生长至关重要，但要求并不完全一致。一般养殖鱼类的最大适应范围为pH值6-9.5，以7.5-8.5为最适；蚌类的最大适应范围为pH值6.5-8.5，以7-8为最适；。当pH值在5以下或10以上时，对鱼、蚌生长都有害，不宜作养殖用水。(5) The pH value of water mainly depends on the ratio of dissolved carbon dioxide and carbonate in the water. Generally speaking, the more carbon dioxide content, the more acidic the water; otherwise, the more alkaline the water. The pH of water is crucial to the growth of fish, mussels and other aquatic organisms, but the requirements are not completely consistent. Generally, the maximum adaptation range of farmed fish is pH 6-9.5, with 7.5-8.5 being the most suitable; the maximum adaptation range of mussels is pH 6.5-8.5, with 7-8 being the most suitable; When the pH value is below 5 or above 10, it is harmful to the growth of fish and mussels, and it is not suitable for aquaculture water.
水族箱水质的传统检测方法除了pH值测试有试纸外，其他指标大都以液体试剂形式供应。液体试剂检测水质操作相对试纸而言较复杂，成本也相对较高，但相比水质监测仪器方便携带，成本低。市面上也有一些专业的水质监测仪器在售，可以快速检测各项指标，但价格高成为普及使用的最主要限制条件。这些传统方法或因耗时稍长，或因成本较高，不适于水族饲养者普及使用，因此水族箱水质安全的快速、灵敏检测是迫切需要解决的关键技术难题。In addition to the test paper for the pH value test, the traditional detection methods of aquarium water quality are mostly supplied in the form of liquid reagents. Compared with test paper, liquid reagents are more complicated to detect water quality, and the cost is relatively high, but compared with water quality monitoring instruments, they are more convenient to carry and lower in cost. There are also some professional water quality monitoring instruments on the market, which can quickly detect various indicators, but the high price has become the most important constraint for popularization. These traditional methods are not suitable for widespread use by aquarium breeders either because they are time-consuming or high in cost. Therefore, rapid and sensitive detection of aquarium water quality safety is a key technical problem that needs to be solved urgently.
自20世纪初以来，一项重大的革命是把纸应用于分析实验中，1952年Martin和Synge因为发明纸色谱法被授予诺贝尔奖。纸成为了一个非常令人关注的平台，并且具有独特的性能，包括：制造和使用成本低廉、具有生物降解性和生物相容性、易涂层印迹、多孔纤维结构、凭借毛细管力无需其他动力、通常为白色可提供比色法和荧光法检测的最佳平台。目前，专利200420070366.8公布了水质总硬度的快速检测试纸，专利CN203310759U公布了水中氯气的快速检测试纸，也有专利CN102928369A公布了亚硝酸盐快速检测试纸，但没有任何一项专利能为水族饲养者提供经济快捷的方法进行全方位的水质检测。因此，必须寻找一种灵敏度高、响应时间短、生产周期短、稳定性强、保存期长、拥有很强的市场竞争力的技术，用来快速检测水族箱中的水质。Since the beginning of the 20th century, a major revolution has been the application of paper to analytical experiments. In 1952, Martin and Synge were awarded the Nobel Prize for their invention of paper chromatography. Paper has emerged as a very interesting platform and has unique properties including: Inexpensive to manufacture and use, Biodegradable and biocompatible, Easy coating imprint, Porous fibrous structure, No need for additional power by virtue of capillary forces , usually white, provides an optimal platform for colorimetric and fluorometric detection. At present, patent 200420070366.8 has announced the rapid detection test paper of total water hardness, patent CN203310759U has announced the rapid detection test paper of chlorine in water, and patent CN102928369A has announced the rapid detection test paper of nitrite, but none of these patents can provide economical benefits for aquarium breeders. A fast way to conduct a full range of water quality testing. Therefore, it is necessary to find a technology with high sensitivity, short response time, short production cycle, strong stability, long shelf life, and strong market competitiveness to quickly detect the water quality in the aquarium.
发明内容Contents of the invention
本发明公开了一种水族箱水质快速检测的6合1试纸，可以方便、快捷地对水族箱中亚硝酸盐、硝酸盐、总硬度、总氯、总碱、pH值进行测定，在1-3min读取检测结果。The invention discloses a 6-in-1 test paper for quickly detecting the water quality of an aquarium, which can conveniently and quickly measure nitrite, nitrate, total hardness, total chlorine, total alkali and pH value in the aquarium. 3min to read the test result.
本发明的组成：包括纸基和6个显色纸片两部分，所述的显色纸片先经过反应液的浸泡、烘干、剪切后和所述的基片固定连接。The composition of the present invention includes two parts: a paper base and 6 color-developing paper sheets. The color-developing paper sheets are fixedly connected to the base sheet after soaking in the reaction solution, drying, and cutting.
本发明的的制备步骤：Preparation steps of the present invention:
(1)反应液的配制：配制反应液1-6，其中反应液1分为反应液1A和反应液1B，反应液1A的溶剂为水，溶质为0.1-0.2g/L氯化钠，反应液1B的溶剂为乙醇，溶质为0.015-0.025g/L磺胺、0.004-0.005g/L N-1(奈基)乙二胺二盐酸盐、0.5-0.8g/L 5-磺基水杨酸和0.5-1.0g/L聚乙烯吡咯烷酮；反应液2分为反应液2A和反应液2B，反应液2A的溶剂为水，溶质为0.1-0.2g/L氯化钠，反应液2B的溶剂为乙醇，溶质为0.015-0.025g/L磺胺、0.004-0.005g/L N-1(奈基)乙二胺二盐酸盐、0.5-0.8g/L 5-磺基水杨酸和0.5-1.0g/L聚乙烯吡咯烷酮；反应液3的溶剂为80-120ml磷酸缓冲液(pH＝10)，溶质为0.3-0.5％铬黑T、0.1-0.2g聚乙烯吡咯烷酮、0.05-0.10％茜素；反应液4的溶剂为80-120ml的20％乙醇溶液，溶质为0.2-0.4％溴甲粉绿、0.1-0.2g聚乙烯吡咯烷酮；反应液5的溶剂为80-120ml的无水乙醇，溶质为2-6g/L二苯甲酮硫代物，3-5g/L聚乙烯吡咯烷酮；反应液6的溶剂为80-120ml的30％乙醇溶液，溶质为溴麝香草酚蓝0.6-0.8g/L、麝香草酚蓝0.3-0.5g/L、酸性间胺黄0.2-0.3g/L、间甲基红0.15-0.25g/L、溴甲酚绿0.05-0.08g/L、硝氮黄0.12-0.22g/L、邻甲酚酜酞0.4-0.5g/L、酚酞0.03-0.05g/L、对硝基苯偶氮水杨酸钠0.8-1.0g/L、达旦黄0.4-0.6g/L、荧光橙红0.02-0.08g/L。(1) Preparation of reaction solution: prepare reaction solution 1-6, wherein reaction solution 1 is divided into reaction solution 1A and reaction solution 1B, the solvent of reaction solution 1A is water, and solute is 0.1-0.2g/L sodium chloride, reacts The solvent of solution 1B is ethanol, and the solute is 0.015-0.025g/L sulfonamide, 0.004-0.005g/L N-1(naphthyl)ethylenediamine dihydrochloride, 0.5-0.8g/L 5-sulfosalicyl Acid and 0.5-1.0g/L polyvinylpyrrolidone; reaction solution 2 is divided into reaction solution 2A and reaction solution 2B, the solvent of reaction solution 2A is water, the solute is 0.1-0.2g/L sodium chloride, and the solvent of reaction solution 2B It is ethanol, the solute is 0.015-0.025g/L sulfonamide, 0.004-0.005g/L N-1 (naphthyl) ethylenediamine dihydrochloride, 0.5-0.8g/L 5-sulfosalicylic acid and 0.5- 1.0g/L polyvinylpyrrolidone; the solvent of reaction solution 3 is 80-120ml phosphate buffer (pH=10), the solute is 0.3-0.5% chrome black T, 0.1-0.2g polyvinylpyrrolidone, 0.05-0.10% alizarin The solvent of reaction solution 4 is 20% ethanol solution of 80-120ml, solute is 0.2-0.4% methyl bromide powder green, 0.1-0.2g polyvinylpyrrolidone; The solvent of reaction solution 5 is dehydrated alcohol of 80-120ml, solute 2-6g/L benzophenone sulfide, 3-5g/L polyvinylpyrrolidone; the solvent of reaction solution 6 is 80-120ml of 30% ethanol solution, and the solute is bromothymol blue 0.6-0.8g/L , Thymol Blue 0.3-0.5g/L, Acid Metamine Yellow 0.2-0.3g/L, Metamethyl Red 0.15-0.25g/L, Bromocresol Green 0.05-0.08g/L, Nitrazine Yellow 0.12- 0.22g/L, o-cresol phthalein 0.4-0.5g/L, phenolphthalein 0.03-0.05g/L, sodium p-nitrophenyl azosalicylate 0.8-1.0g/L, Dadan yellow 0.4-0.6g/L , Fluorescent orange red 0.02-0.08g/L.
(3)滤纸的裁剪：用裁纸器把3mm层析滤纸(以下简称滤纸)裁剪成10cm×15cm的矩形纸片，便于后续浸液和烘干；(3) Cutting of filter paper: use a paper cutter to cut 3mm chromatography filter paper (hereinafter referred to as filter paper) into rectangular paper sheets of 10cm × 15cm, which is convenient for subsequent immersion and drying;
(4)反应液的浸泡：把配置好的反应液1-6倒入专用玻璃皿中，用镊子夹住滤纸1-6分别在反应液1-6中浸泡10-20s，用夹子挂在干燥箱板上；(4) Soaking of the reaction solution: Pour the prepared reaction solution 1-6 into a special glass dish, clamp the filter paper 1-6 with tweezers and soak in the reaction solution 1-6 for 10-20s respectively, hang it on a dry box board;
(5)滤纸的烘干：为防止空气中的干扰使试纸降低使用功能，将完成浸液的滤纸放入真空干燥箱内，达到真空度100-110Pa进行烘干。其中1号纸片事先经过反应液1A，于温度90-100℃、真空度100-110Pa的环境下烘干20-30min；在经过反应液1B的浸泡，于温度50-60℃、真空度100-110Pa的环境下烘干20-30min。2号纸片事先经过反应液2A，于温度90-100℃、真空度100-110Pa的环境下烘干20-30min；在经过反应液2B的浸泡，于温度50-60℃、真空度100-110Pa的环境下烘干20-30min，再在其表面均匀涂抹一薄层处理过的无砷锌粉，用冷风吹干。3号纸片于温度50-60℃、真空度100-110Pa的环境下烘干30-40min。4号纸片于温度50-60℃、真空度100-110Pa的环境下烘干30-40min。5号纸片于温度50-60℃、真空度100-110Pa的环境下烘干30-40min。6号纸片于温度50-60℃、真空度100-110Pa的环境下烘干30-40min。(5) Drying of filter paper: In order to prevent the interference in the air from reducing the function of the test paper, put the soaked filter paper into a vacuum drying box, and dry it at a vacuum degree of 100-110Pa. Among them, the No. 1 paper sheet was dried in the reaction solution 1A in advance at a temperature of 90-100°C and a vacuum of 100-110Pa for 20-30min; Dry at -110Pa for 20-30min. Paper No. 2 was dried in the reaction solution 2A in advance at a temperature of 90-100°C and a vacuum of 100-110Pa for 20-30min; Dry at 110Pa for 20-30min, then apply a thin layer of treated arsenic-free zinc powder evenly on the surface, and dry it with cold wind. Paper No. 3 was dried for 30-40 minutes at a temperature of 50-60°C and a vacuum of 100-110Pa. Paper No. 4 was dried for 30-40 minutes at a temperature of 50-60°C and a vacuum of 100-110Pa. Paper No. 5 was dried for 30-40 minutes at a temperature of 50-60°C and a vacuum of 100-110Pa. Paper No. 6 was dried for 30-40 minutes at a temperature of 50-60°C and a vacuum of 100-110Pa.
(6)显色纸片的贮藏：干燥好的滤纸1-6即为显色纸片1-6号，将其裁剪成1cm×15cm的显色条1-6号，每张可裁剪为10条，封在自封袋内，放入盛有干燥剂的干燥器中留存待检；(6) Storage of color-developing paper sheets: the dried filter paper 1-6 is color-developing paper sheet No. 1-6, which is cut into 1cm×15cm color-developing strip No. strips, sealed in a ziplock bag, put into a desiccator filled with desiccant and kept for inspection;
(7)纸基的制作：用裁纸器把PP合成纸裁剪成7cm×15cm的矩形纸片，便于后续纸基和显色片的黏合和试纸条的切割。(7) Production of the paper base: Cut the PP synthetic paper into a 7cm×15cm rectangular piece of paper with a paper cutter, which is convenient for the subsequent bonding of the paper base and the chromogenic film and the cutting of the test strip.
(8)纸基和显色片的黏合：将裁剪好的1cm×15cm的显色纸片1-6，用粘合剂黏合在7cm×15cm的纸基片上，成为试纸片；(8) Bonding of the paper base and the chromogenic sheet: the chromogenic paper sheets 1-6 of 1 cm×15 cm that have been cut are glued on the paper base sheet of 7 cm×15 cm with an adhesive to become a test paper sheet;
(9)试纸条的切割：用切纸机将黏合好的试纸片均匀切割为0.55cm×7cm的试纸条；(9) Cutting of test strips: Use a paper cutter to evenly cut the bonded test strips into 0.55cm×7cm test strips;
(10)试纸条的贮藏：密封在4-20℃干燥环境内贮存，不得受到阳光直射。(10) Storage of test strips: sealed and stored in a dry environment at 4-20°C, and should not be exposed to direct sunlight.
本发明的适用范围：水族箱水质的亚硝酸盐、硝酸盐、总硬度、总氯、总碱、pH值6项指标快速检测，属于水质安全监测领域。The scope of application of the present invention: the rapid detection of six indicators of nitrite, nitrate, total hardness, total chlorine, total alkali and pH value of the water quality of an aquarium belongs to the field of water quality safety monitoring.
本发明的检测范围：亚硝酸盐0-250mg/L、硝酸盐0-40mg/L、总硬度0-425mg/L、总氯0-100mg/L、总碱0-240mg/L、pH值6.5-8.5。Detection range of the present invention: nitrite 0-250mg/L, nitrate 0-40mg/L, total hardness 0-425mg/L, total chlorine 0-100mg/L, total alkali 0-240mg/L, pH 6.5 -8.5.
本发明的操作方法：取适量的水样于洁净的干燥容器，以浸没试纸的显色片区域为标准，浸没后立即取出，将显色片朝上水平放置，可在1-3min后进行定量判定。The operation method of the present invention: take an appropriate amount of water sample in a clean dry container, take the area of the chromogenic film immersed in the test paper as the standard, take it out immediately after immersion, place the chromogenic film upwards and horizontally, and perform quantification after 1-3 minutes determination.
本发明的结果判定：将试纸的颜色与标准比色卡进行比较进行初步判定，与比色卡颜色相同的色阶对应的数值即为本样品的检测值，如试纸的颜色在两个色阶之间即取两者的中间值。Result judgment of the present invention: compare the color of the test paper with the standard color comparison card for preliminary judgment, and the numerical value corresponding to the color scale identical to the color of the color comparison card is the detection value of this sample, such as the color of the test paper in two color levels Take the intermediate value between the two.
本发明的特点：仅由纸基和显色纸片两部分组成，结构和操作都很简单；以3mm层析滤纸为载体，使试纸能够在1-3min内对水族箱水质的亚硝酸盐、硝酸盐、总硬度、总氯、总碱、pH值等6项指标快速检测；依靠目力观察即可得出准确的定量检测结论，不需用贵重仪器显示，使用者不需专门训练就能掌握；同时价格便宜，生产成本低，为一次性使用且无需检修维护，精确度较高，重复性也好，非常适宜于现场实时快速检测和监测。Features of the present invention: it only consists of paper base and color-developing paper, and its structure and operation are very simple; 3mm chromatographic filter paper is used as a carrier, so that the test paper can detect nitrite, Rapid detection of 6 indicators such as nitrate, total hardness, total chlorine, total alkali, and pH value; accurate quantitative detection conclusions can be drawn by visual observation, no need for expensive instruments to display, and users can master it without special training ; At the same time, the price is cheap, the production cost is low, it is one-time use and does not need maintenance, and the accuracy is high and the repeatability is good. It is very suitable for real-time rapid detection and monitoring on site.
附图说明Description of drawings
图1为本发明的俯视结构示意图；Fig. 1 is the top view structure schematic diagram of the present invention;
图2为本发明的侧视结构示意图；Fig. 2 is the side view structure schematic diagram of the present invention;
图3为本发明的标准比色卡(mg/L)，比色对应十六进制颜色码。Fig. 3 is the standard color comparison card (mg/L) of the present invention, and color comparison corresponds to the hexadecimal color code.
具体实施方式Detailed ways
制备实施例Preparation Example
实施例1：水质亚硝酸盐的检测试纸Embodiment 1: the detection test paper of water quality nitrite
首先配制反应液1A和反应液1B，反应液1A中的溶剂为水，溶质为氯化钠0.1g/L；反应液1B中的。将3mm层析滤纸在反应液1A中匀速浸过，在真空度100Pa、温度100℃的环境下烘干25溶剂为乙醇，溶质为磺胺0.015g/L、N-1(奈基)乙二胺二盐酸盐0.004g/L、5-磺基水杨酸0.5g/L和聚乙烯吡咯烷酮0.5g/Lmin，然后在反应液1B中匀速浸过，于温度60℃、真空度100Pa的环境烘干20min。将处理好的试纸片密封在放入盛有干燥剂的干燥器中，不得受到阳光直射。First prepare reaction solution 1A and reaction solution 1B, the solvent in reaction solution 1A is water, the solute is sodium chloride 0.1g/L; in reaction solution 1B. Immerse 3mm chromatographic filter paper in the reaction solution 1A at a constant speed, and dry it at a vacuum degree of 100Pa and a temperature of 100°C for 25 minutes. Dihydrochloride 0.004g/L, 5-sulfosalicylic acid 0.5g/L and polyvinylpyrrolidone 0.5g/Lmin were soaked in the reaction solution 1B at a uniform speed, and dried at a temperature of 60°C and a vacuum of 100Pa. Dry for 20 minutes. Seal the processed test strips in a desiccator filled with desiccant, and keep them away from direct sunlight.
实施例2：水质硝酸盐的检测试纸Embodiment 2: the detection test paper of water quality nitrate
首先配制反应液2A和反应液2B，反应液2A中的溶剂为水，溶质为氯化钠0.1g/L；反应液2B中的溶剂为乙醇，溶质为磺胺0.015g/L、N-1(奈基)乙二胺二盐酸盐0.004g/L、5-磺基水杨酸0.5g/L和聚乙烯吡咯烷酮0.5g/L。将3mm层析滤纸在反应液A中匀速浸过，在真空度100Pa、温度100℃的环境下烘干25min，然后在反应液B中匀速浸过，再在其一面均匀涂抹一薄层处理过的无砷锌粉，用冷风吹干。将处理好的试纸片密封在放入盛有干燥剂的干燥器中，不得受到阳光直射。First prepare reaction solution 2A and reaction solution 2B, the solvent in reaction solution 2A is water, and solute is sodium chloride 0.1g/L; The solvent in reaction solution 2B is ethanol, and solute is sulfonamide 0.015g/L, N-1( Nathyl) ethylenediamine dihydrochloride 0.004g/L, 5-sulfosalicylic acid 0.5g/L and polyvinylpyrrolidone 0.5g/L. Soak the 3mm chromatography filter paper in the reaction solution A at a constant speed, dry it for 25 minutes at a vacuum degree of 100Pa and a temperature of 100°C, then soak it in the reaction solution B at a constant speed, and then apply a thin layer of treated paper on one side evenly Arsenic-free zinc powder, dry with cold air. Seal the treated test strips in a desiccator filled with desiccant, and keep them away from direct sunlight.
实施例3：水质总硬度的检测试纸Embodiment 3: the detection test paper of water quality total hardness
首先配制0.5％的铬黑T和0.05％的茜素混合溶液，用pH＝10的磷酸缓冲液定容至100ml；添加0.2g乙烯吡咯烷酮得到反应液。将3mm层析滤纸在反应液中浸泡，浸泡时间为20s，于温度50℃、真空度100-110Pa的环境下烘干30min。将处理好的试纸片密封在放入盛有干燥剂的干燥器中，不得受到阳光直射。Firstly, a mixed solution of 0.5% chrome black T and 0.05% alizarin was prepared, and the volume was adjusted to 100 ml with a phosphate buffer solution of pH=10; 0.2 g of vinylpyrrolidone was added to obtain a reaction solution. Soak 3mm chromatographic filter paper in the reaction solution for 20s, and dry for 30min at a temperature of 50°C and a vacuum of 100-110Pa. Seal the processed test strips in a desiccator filled with desiccant, and keep them away from direct sunlight.
实施例4：水质总碱的检测试纸Embodiment 4: the detection test paper of water quality total alkali
首先配置0.2％的溴甲粉绿溶液，溶剂为100ml的20％乙醇溶液；添加0.2g乙烯吡咯烷酮得到反应液。将3mm层析滤纸在反应液中浸泡，浸泡时间为20s，于温度60℃、真空度100-110Pa的环境下烘干30min。将处理好的试纸片密封在放入盛有干燥剂的干燥器中，不得受到阳光直射。Firstly, a 0.2% methyl bromide green solution is prepared, and the solvent is 100 ml of 20% ethanol solution; 0.2 g of vinylpyrrolidone is added to obtain a reaction solution. Soak 3mm chromatographic filter paper in the reaction solution for 20s, and dry for 30min at a temperature of 60°C and a vacuum of 100-110Pa. Seal the processed test strips in a desiccator filled with desiccant, and keep them away from direct sunlight.
实施例5：水质氯气的检测试纸Embodiment 5: the detection test paper of water quality chlorine
首先制备溶剂为无水乙醇，溶质为2g/L二苯甲酮硫代物，3g/L聚乙烯吡咯烷酮。将3mm层析滤纸在反应液中浸泡，浸泡时间为20s，于温度60℃、真空度100-110Pa的环境下烘干30min。将处理好的试纸片密封在放入盛有干燥剂的干燥器中，不得受到阳光直射。Firstly, the solvent is absolute ethanol, the solute is 2 g/L benzophenone thio, and 3 g/L polyvinylpyrrolidone. Soak 3mm chromatographic filter paper in the reaction solution for 20s, and dry for 30min at a temperature of 60°C and a vacuum of 100-110Pa. Seal the processed test strips in a desiccator filled with desiccant, and keep them away from direct sunlight.
实施例6：水质PH的检测试纸Embodiment 6: the detection test paper of water quality PH
首先制备溶剂为30％的乙醇，溶质为溴麝香草酚蓝0.8g/L、麝香草酚蓝0.5g/L、酸性间胺黄0.3g/L、间甲基红0.25g/L、溴甲酚绿0.05g/L、硝氮黄0.12g/L、邻甲酚酜酞0.4g/L、酚酞0.03g/L、对硝基苯偶氮水杨酸钠0.8g/L、达旦黄0.4g/L、荧光橙红0.02g/L。将3mm层析滤纸在反应液中浸泡，浸泡时间为20s，于温度50℃、真空度100-110Pa的环境下烘干30min。将处理好的试纸片密封在放入盛有干燥剂的干燥器中，不得受到阳光直射。Firstly, the preparation solvent is 30% ethanol, and the solute is bromothymol blue 0.8g/L, thymol blue 0.5g/L, acid metamine yellow 0.3g/L, metamethyl red 0.25g/L, methyl bromide Phenol Green 0.05g/L, Nitrazine Yellow 0.12g/L, O-Cresol Phenphthalein 0.4g/L, Phenolphthalein 0.03g/L, Sodium p-Nitrophenylazosalicylate 0.8g/L, Dadan Yellow 0.4g /L, fluorescent orange red 0.02g/L. Soak 3mm chromatographic filter paper in the reaction solution for 20s, and dry for 30min at a temperature of 50°C and a vacuum of 100-110Pa. Seal the processed test strips in a desiccator filled with desiccant, and keep them away from direct sunlight.
实施例7：水族箱水质的检测试纸Embodiment 7: the detection test paper of aquarium water quality
用裁纸器把PP合成纸裁剪成7cm×15cm的矩形纸片作为基板，将显色纸片1-6号分别裁剪为1cm×15cm的长条并依此贴于基板，成为试纸片；用切纸机将黏合好的试纸片均匀切割为0.55cm×7cm的试纸条，密封在4-20℃干燥环境内贮存，不得受到阳光直射(图1和图2)。Use a paper cutter to cut PP synthetic paper into 7cm×15cm rectangular pieces of paper as the substrate, and cut the chromogenic paper No. 1-6 into strips of 1cm×15cm and paste them on the substrate accordingly to become test paper pieces; Cut the bonded test paper into 0.55cm×7cm strips with a paper cutter, and store them in a dry environment at 4-20°C without direct sunlight (Figure 1 and Figure 2).
检测实施例Detection example
实施例8：Embodiment 8:
对3个花鸟市场在售的水族箱水质进行采样检测，分别取适量样品于洁净的干燥容器，以浸没试纸片为标准，3min后将试纸的颜色与标准比色卡进行比较，与比色卡颜色相同的色阶对应的数值即为本样品的检测值，如试纸的颜色在两个色阶之间即取两者的中间值。其中一个水族箱内的水样亚硝酸盐含量为100mg/L，水的硬度为120mg/L，存在不利于水族生物的生长的因素。其他两个水族箱水质状况良好，是水族生物的理想生存条件。Sampling and testing the water quality of the aquariums sold in the 3 flower and bird markets, taking appropriate amount of samples in clean dry containers, using the submerged test paper as the standard, and comparing the color of the test paper with the standard color card after 3 minutes, and comparing the color of the test paper with the color card The value corresponding to the color scale of the same color is the detection value of this sample. If the color of the test paper is between the two color scales, the middle value of the two is taken. The nitrite content of the water sample in one of the aquariums is 100mg/L, and the water hardness is 120mg/L, which is not conducive to the growth of aquatic organisms. The other two aquariums have good water quality and are ideal living conditions for aquatic organisms.
实施例9：Embodiment 9:
对3个水族生物饲养爱好者使用的水族箱水质进行采样检测，分别取适量样品于洁净的干燥容器，以浸没试纸片为标准，3min后将试纸的颜色与标准比色卡进行比较，与比色卡颜色相同的色阶对应的数值即为本样品的检测值，如试纸的颜色在两个色阶之间即取两者的中间值。其中一位水族爱好者水族箱内的水样亚硝酸盐含量为50mg/L，水的硬度为250mg/L；另一位水族爱好者水族箱内的水样总氯含量为25mg/L；第三位水族爱好者水族箱内的pH8.5，总碱大于240mg/L。均存在不利于水族生物的生长的因素。Sampling and testing the water quality of the aquariums used by 3 aquarium breeding enthusiasts, taking appropriate amount of samples in clean dry containers, taking the immersion test paper as the standard, and comparing the color of the test paper with the standard color comparison card after 3 minutes The value corresponding to the color scale of the same color of the color card is the detection value of this sample. If the color of the test paper is between the two color scales, the middle value of the two is taken. The nitrite content of the water sample in the aquarium of one of the aquarium enthusiasts was 50mg/L, and the water hardness was 250mg/L; the total chlorine content of the water sample in the aquarium of another aquarium enthusiast was 25mg/L; The pH in the aquarium of three aquarium enthusiasts is 8.5, and the total alkali is greater than 240mg/L. There are factors that are unfavorable for the growth of aquatic organisms.
根据中华人民共和国水产行业标准SC/T 9411-2012《水族馆水生哺乳动物饲养水质》对上述6份样品进行验证，检测结果与本发明的试纸结果一致，证明了本发明的实用性和准确性。According to the People's Republic of China Aquatic Industry Standard SC/T 9411-2012 "Aquarium Aquatic Mammal Breeding Water Quality", the above-mentioned 6 samples are verified, and the detection result is consistent with the test paper result of the present invention, which proves the practicability and accuracy of the present invention .
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510293980.3ACN104897868A (en) | 2015-06-02 | 2015-06-02 | Six-in-one test paper for fast detecting quality of water in aquarium |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510293980.3ACN104897868A (en) | 2015-06-02 | 2015-06-02 | Six-in-one test paper for fast detecting quality of water in aquarium |
| Publication Number | Publication Date |
|---|---|
| CN104897868Atrue CN104897868A (en) | 2015-09-09 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510293980.3APendingCN104897868A (en) | 2015-06-02 | 2015-06-02 | Six-in-one test paper for fast detecting quality of water in aquarium |
| Country | Link |
|---|---|
| CN (1) | CN104897868A (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181690A (en)* | 2015-09-28 | 2015-12-23 | 北京福德安科技有限公司 | 12-channel micro-flow rapid colorimetric water quality detection device |
| CN107228856A (en)* | 2017-08-11 | 2017-10-03 | 中国锅炉水处理协会 | A kind of boiler feedwater quality hardness test agent and its preparation |
| CN107271469A (en)* | 2017-06-08 | 2017-10-20 | 上海市环境科学研究院 | The heavy metal in water semi-quantitative analysis method analyzed based on X-ray fluorescence spectra |
| CN108072653A (en)* | 2016-11-28 | 2018-05-25 | 上海泓宝绿色水产股份有限公司 | A kind of total hardness detecting system and its application method for being used to detect that total hardness is 0-560mg/L |
| CN108593640A (en)* | 2018-04-19 | 2018-09-28 | 长春万成生物电子工程有限公司 | A kind of Test paper of water quality total alkalinity and preparation method thereof and detection method |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181690A (en)* | 2015-09-28 | 2015-12-23 | 北京福德安科技有限公司 | 12-channel micro-flow rapid colorimetric water quality detection device |
| CN105181690B (en)* | 2015-09-28 | 2018-03-27 | 北京福德安科技有限公司 | A kind of quick colorimetric water quality detecting device of 12 passage miniflows |
| CN108072653A (en)* | 2016-11-28 | 2018-05-25 | 上海泓宝绿色水产股份有限公司 | A kind of total hardness detecting system and its application method for being used to detect that total hardness is 0-560mg/L |
| CN107271469A (en)* | 2017-06-08 | 2017-10-20 | 上海市环境科学研究院 | The heavy metal in water semi-quantitative analysis method analyzed based on X-ray fluorescence spectra |
| CN107228856A (en)* | 2017-08-11 | 2017-10-03 | 中国锅炉水处理协会 | A kind of boiler feedwater quality hardness test agent and its preparation |
| CN108593640A (en)* | 2018-04-19 | 2018-09-28 | 长春万成生物电子工程有限公司 | A kind of Test paper of water quality total alkalinity and preparation method thereof and detection method |
| Publication | Publication Date | Title |
|---|---|---|
| US4587213A (en) | Methods and means of determining microorganism population | |
| CN104897868A (en) | Six-in-one test paper for fast detecting quality of water in aquarium | |
| US3814670A (en) | Test article for use in microbiology | |
| Kiryukhin et al. | A membrane film sensor with encapsulated fluorescent dyes towards express freshness monitoring of packaged food | |
| JP2007278926A (en) | Detector and detection method | |
| CN103439322B (en) | The rapid assay methods that a kind of Peracetic acid is residual | |
| CN104568929A (en) | Rapid testing paper for testing hydrogen peroxide residue in foods | |
| CN114113063A (en) | A kind of preparation method and application of fish freshness indicator colorimetric array | |
| CN113186613A (en) | Nanofiber indicating film for intelligently detecting marine fish spoilage and application thereof | |
| CN104076034A (en) | RGB quantitative quick detecting test paper for formaldehyde residue in food | |
| CN113402734A (en) | Preparation method and application of hydrogel for indicating freshness of chilled meat | |
| DE60218877T2 (en) | METHOD AND DEVICE FOR MEASURING THE ALDEHYDE CONTENT IN A POLYESTER POLYMER | |
| CN105651765A (en) | Rapid protein test paper, and production method and detection method thereof | |
| CN104749175A (en) | Method for detecting organophosphorus pesticide residues in fruits and vegetables | |
| Dromgoole | The effects of pH and inorganic carbon on photosynthesis and dark respiration of Carpophyllum (Fucales, Phaeophyceae) | |
| CN107764810A (en) | A kind of quick detection test paper of Nitrite and preparation method thereof | |
| CN207663666U (en) | A kind of intelligent color-changing indicating label for judging fresh white flower fish freshness | |
| CN113418913A (en) | Stable nitrite test paper and preparation method thereof | |
| US11852638B2 (en) | Calcium ion concentration measuring device | |
| CN117672073A (en) | Amine-sensitive food freshness indicating label and preparation method thereof | |
| CN112730392A (en) | Test strip for rapidly detecting acid value of salad dressing, preparation method and test method | |
| CN110129403A (en) | A method for detecting the toxicity of luminescent bacteria | |
| FI71164C (en) | PROCEDURE FOR THE PURPOSE OF A MICRO-ORGANISM | |
| EP3036541B1 (en) | A chlorine analytical test element, stabilized n,n-diethyl-p-phenylenediamine solution and method for making the test element | |
| CN104502342A (en) | Zinc ion test paper as well as preparation method and application thereof |
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication | Application publication date:20150909 |