A kind of rhamnolipid personal cleanliness articles for use and preparation method thereofTechnical field
The present invention relates to technical field prepared by hygienic cleansing product, and in particular to one kind prepares cleaning using rhamnolipidThe technical field of articles for use.
Background technology
The mankind just understand since ancient times, and Body Comfort Index can not only be improved by being particular about personal hygiene, even more prevent diseaseThe necessary condition that disease is kept fit, clean is also beautiful premise, and workplace youth and people seeking beauty of today especially lie in aThe clean hygiene of people, keeps the clean salubrious of skin surface and hair, can also externally establish the personal image of good health, carryRise self-confident.As it can be seen that personal cleanliness's work is not once and for all, it is necessary to daily all carry out, because on our skins and hairDirt constantly produces, and dirt is essentially from aging exuviae skin cell debris, the sweat of sweat gland discharge, sebaceous glands dischargeSebum and be adhered to the dust of body surface.
Due to the presence of sebum so that these dirts are difficult to be cleaned thoroughly with clear water, this is a common recognition out of question, it is formerBecause being that grease is clearly demarcated, between the two without compatibility, therefore by the amphipathic of surfactant, develop clear to help to improveThe efficiency of dirt is purpose personal cleanliness's articles for use, it appears necessary.
Currently, personal cleanliness's articles for use that in the market has been released, from the time sequencing and core functional component of appearance notIt is same to be broadly divided into three generations：The first generation, early stage, the mankind mainly used, from for example long gleditsia sinensis of natural plant raw material, circle gleditsia sinensis,And the fruit such as tea seed cake powder, wherein saponin(e is the main composition with cleaning dirt digestion；The second generation, the modern age mankindIt is main to use, be total to the fertile perfumed soap that heat boils from grease and the alkali of animal, wherein soap is that have to clean decontaminationThe main composition of effect；The third generation, at present the mankind mainly use, from petrochemical by-product commercial synthesis table liveAgent, such as stearic acid, Sulfonates, quaternary ammonium salt, betaines, polyethers, fatty acid glycerine esters etc..
The crowd used before the personal cleansing items of first generation natural plant raw material is considerably less, due to there isThe shortcomings that mucous membrane irritation is strong and inconvenient to use；Second generation fertilizer perfumed soap class cleaning supplies are used for the cleaning of individual's skin and hairWhen, the shortcomings that there is tight rear skin and alkaline strong injury skin is washed；Third generation chemical industry synthesis detergent, the drawback is that chemical industryBuilding-up process high energy consumption high pollution, and it is not thorough the murder by poisoning thing with inevitable side reaction generation in the prior art in the presence of reactionMatter risk, synthetic product is complicated to be not easy to be degraded, and there is the secondary pollution to water body, while the thickening in formula after useThe materials such as agent, brightening agent, preservative are very important social concerns for the residual and then influence health of people of user.
At present, commercially with cleaning skin and hair personal cleanliness's articles for use as the main purpose, to utilize microorganismThe pure biosurfactant that fermentation technique obtains is the product of unique functional component, is had not been reported.Disclosed in Chinese patentIn the detergent compositions proposed in these documents such as number 102399644 A/CN of CN, 103052704 A/CN, 104490641 AWhen adding a small amount of rhamnolipid raw material, all need to compound wide variety of conventional chemical industry synthesis class surfactant or enzyme preparation, Cai Nengda at the same timeTo cleaning effect, and it is typically to be used for membrane module, the washing of fruits and vegetables or textile fabric, does not propose to lead in personal cleanliness's articles for useThe application in domain, meanwhile, it is well known that due to rhamnolipid crude extract color and luster and smell is bad brings final cleaning product notSharp factor needs to solve as early as possible, therefore the detergent containing rhamnolipid of these generalities and is not suitable for personal bodyThe cleaning of table grease.
The content of the invention
Prepared for having no at present purely using rhanolipid as biosurfactant as unique core functional component aThe state of the art of people's cleaning supplies, and the shortcomings that existing human body washing and skin care product and deficiency, the present invention intends to provide oneKind rhamnolipid personal cleanliness's articles for use, it then follows cleaning effect is notable, simplifies formula, prevents harmful preservative, thickener, brightening agentDeng the minimalist art theory of auxiliary material, with reference to rhamnolipid fermentation technique, prepared using the rhamnolipid raw material for producing high-qualitySuitable for personal cleaning supplies, i.e., the pure biological agent using rhanolipid as biosurfactant as unique decontamination functional componentType personal cleanliness's articles for use, have the characteristics that cleaning is thorough and safe and healthy environmentally friendly, are consumers in general's desires of the people, have realityPracticality.
To reach above-mentioned purpose, the present invention adopts the following technical scheme that：
By highly concentrated biosurfactant plaster shape rhamnolipid using after fermented Hydrolysis kinetics as uniquelyDecontamination functional component, using citric acid as pH adjusting agent, using Peppermint Water as deodorant tune, using distilled water or deionized water as final creamBody viscosity modifier, prepares personal cleanliness's articles for use, according to filling side using mixing standing degassing is sufficiently stirred after direct additionJust the needs of property and shelf-life, consider the rhamnolipid content of final products between 50%-90%, prioritizing selection 70%-80% scope, lotion is too hard inconvenient filling and uses, and lotion is too soft or in flow-like then limited shelf life.According to storage barPart and the difference of use occasion, the optimum formula of personal cleanliness's articles for use is finally obtained by contrast experiment repeatedly, and its is preparedTechnique, by repeatedly testing, testing and trying out, cleaning effect to human skin and washes rear skin sense and all obtains significant skillArt effect.
Specifically, the present invention provides a kind of rhamnolipid personal cleanliness articles for use, it is 90%- to count formula by weight50-95 parts of 95% rhamnolipid condensed cream, 0.1-0.5 parts of citric acid, 0.1-0.5 parts, pH value 5.0-10.0 of Peppermint Water, go fromSub- water supplies 100 parts.
Further, count by weight, the present invention provides what a kind of rhamnolipid personal cleanliness articles for use were preferentially selected to match somebody with somebody70-80 parts of the rhamnolipid condensed cream of Fang Wei, 90%-95%, 0.1-0.3 parts of citric acid, 0.2-0.3 parts of Peppermint Water, pH value 6.0-8.0, deionized water supplies 100 parts.
Rhamnolipid personal cleanliness articles for use are formulated best composition：Count by weight, the rhamnolipid concentration of 90%-95%75 parts of cream, 0.1 part of citric acid, 0.2 part of Peppermint Water, pH value 6.5-7.0 deionized waters supply 100 parts.
In the present invention, the water solubility of the Peppermint Water that uses as deodorant tune for the distillation of fresh natural peppermint plant stem-leafComponent, main component are the pure menthol of fragrance.
The rhamnolipid condensed cream of 90%-95% of the present invention is obtained using following preparation method, by using freshVegetable oil is as carbon source, using pseudomonas aeruginosa as fermenting microbe is fermented, extraction, refined and concentration form, specific stepsIt is as follows：
（1）Spawn incubation：The pseudomonas aeruginosa parent species that a ring newly purifies are scraped, is inoculated in Shake flask medium and cultivates,Specific condition of culture is 35-38 DEG C of temperature, rotating speed 150-180rpm, and culture 40-60h obtains shake-flask seed.
（2）Fermentation：Above-mentioned shake-flask seed is inoculated into fermentation tank, inoculum concentration is the 5-10% of fermentation medium volume, is sent outFerment temperature control is at 35-38 DEG C, without from zymotic fluid bottom ventilation, avoiding the technical barrier of later stage foam overflow, more need notEthanol defoams, mixing speed 20-40rpm, and fermentation time is 5-6 days；In fermentation process, pH can be raised constantly, be utilized every 12hBetween zymotic fluid is recalled to pH6.5-6.8 by 55% citric acid solution, until fermentation ends, obtain yellow green zymotic fluid.
（3）Precipitated product：Citric acid is added in above-mentioned zymotic fluid, adjusting solution ph is 4.0-4.5, stands 10-30min, abandons supernatant, collects bottom sedimentation products, obtains rhamnolipid crude product, content 40-60%.
（4）It is refined：By above-mentioned steps（3）Rhamnolipid precipitation crude product further washes away free citric acid near with clear waterAfter neutrality, redissolve in the soda water of 1-2%, stir to thorough dissolving, pH value of solution and be adjusted between 7.0-7.5, rhamnolipid concentration10-20%, 80-90 DEG C is heated to by solution, and the active carbon particle for adding 3-5% carries out decoloration and deodorization to rhamnolipid crude product, gentlyStirring 1h is rubbed, standing treats that nature cools, with the above-mentioned solution of 100 mesh screens, pH value of solution is recalled to 4.0- again with citric acid4.5, sedimentation products are recycled, and wash precipitation 3 times with clear water, and precipitation is recalled into pH weakly acidic pH 6.8-7.2 with soda, will be above-mentioned heavyConcentration of forming sediment is that rhamnolipid condensed cream content is 85-95%, dense with the above-mentioned rhamnolipid of absolute ethyl alcohol extraction under the conditions of -20 DEG CContracting cream, extracts 3 times, merges above-mentioned ethanol solution and filter, and then rotated evaporimeter recycling ethanol, acquisition contains rhamnolipidThe refined rhamnolipid condensed cream of 90-95%.
Above-mentioned steps（1）In, the shake-flask seed culture medium composition is as follows：10 ~ 20g/L of fresh plant oil;NaNO33~5g/L;K2HPO41.5~2g/L;KH2PO40.7~1g/L;MgSO40.3~0.5g/L；0.1 ~ 0.2g/L of yeast extract, surplus are water.
Above-mentioned steps（2）In, the fermentation medium composition is as follows：25 ~ 35g/L of fresh plant oil;NaNO33~5g/L;K2HPO41.5~2g/L;KH2PO40.7~1g/L;MgSO40.3~0.5g/L；0.05 ~ 0.1g/L of yeast extract, surplus are water.
Above-mentioned steps（5）（6）In, the fresh plant oil can be the peanut oil of fresh squeezing, walnut oil, olive oil,Apricot kernel oil, sesame oil, soybean oil, rapeseed oil, sunflower oil, maize germ oil, safflower seed oil etc., but it is not limited to above-mentioned vegetable oilFat.
Above-mentioned steps（2）In, the fermentation tank is not conventional upright gas-lifting type stirred fermentor, but is used non-perpendicularInclination tank body, and by the driving tank body rotation of tank base motor, tank base is equipped with fixed paddle, and add includingBut the customary secondary equipment such as the probe such as temperature common on fermentation tank, pH, dissolved oxygen and liquid level and corresponding control device is not limited to,Therefore it can realize that the stirring under the conditions of the slow-speed of revolution is ventilated and sufficient dissolved oxygen effect, save the cost of power consumption.
The pseudomonas aeruginosa that the present invention uses is obtained by buying AB93066 from CCTCC, those of ordinary skill in the artIt can be bought and obtained by public's approach.
Rhamnolipid of the present invention is the concentration lotion for being 90%-95% containing rhamnolipid concentration, is with fresh pressureSqueezing plant oil obtains rhamnolipid product for substrate through pseudomonas aeruginosa microbe conversion, the structure of rhamnolipid product be byA variety of rhamnolipid homologues composition based on monose fat and two glycolipids, its common feature is that all have hydrophilic rhamnopyranosylWith lipophilic hydroxyl certain herbaceous plants with big flowers acid groups, pH is in 6.5-7.5 weakly acidic pHs after the extracted refining and concentrating of rhamnolipid product, according to decolorationThe difference of degree, light red is presented to faint yellow, the pure texture as Fresh Honey in appearance, according to the difference of deodorization degreeSpecial fermentative smell is presented to almost tasteless in smell, soaks and rhamnolipid lotion is applied to the centre of the palm after both hands, have after kneading openVery fine and smooth white foam, goes greasy effect totally thorough, and foam is easy to rinse, the greasy feeling of noresidue.
Meanwhile the present invention specifically provides the preparation method of above-mentioned rhamnolipid personal cleanliness articles for use, rhamnolipid is personal clearClean articles for use component is as follows according to weight part ratio meter, specific preparation process：
（1）Use bacterium numbering will be new through above-mentioned non-perpendicular ferment tank culture for the pseudomonas aeruginosa of AB93066Fresh plant oil is converted into rhamnolipid, the mouse that the rhamnolipid content obtained after onestep extraction of going forward side by side, refined, concentration is 90-95%Lee's glycolipid condensed cream, according to weight part ratio meter, the rhamnolipid concentration lotion that 70-80 parts of accurate weighing is to be diluted.
（2）Accurate to weigh 0.1-0.3 parts of citric acids and 0.2-0.3 parts of Peppermint Waters, deionized water supplies 100 parts, obtains 200Gram deionized water solution it is spare.
（3）By step（2）Described in deionized water solution, add step（1）Described in 90%-95% rhamnolipidIn condensed cream, it is slowly stirred to abundant dissolving, it is product to stand after deaerating, and the content of rhamnolipid is 72-76% in product,Shelf life of products is not less than 1 year.
Personal cleanliness's articles for use of present invention are including but not limited to facial cleanser, shampoo, shower cream, toothpaste etc. with cleaningAll detergents used in skin surface and hair.
The rhamnolipid that present invention preferentially uses is monose fat and the composition of two glycolipids, or passes through technologyThe composition of single monose fat, two glycolipids or its arbitrary proportion that means separation obtains is substituted.
The Peppermint Water that present invention preferentially uses can also be peppermint oil known in the art, Rosa Damascana, smoke clothingCareless essential oil, etc. natural perfume material substituted.
The water of present invention preferentially using distilled water or deionized water can also by tap water, well water, etc. natural nothingBacterium water is substituted.
Rhamnolipid is selected directly to prepare rhamnolipid personal cleansing as unique decontamination functional component in the present inventionProduct, are mainly based upon the metabolite that rhamnolipid is transformed by edible vegetable oil and fat through microbial fermentation, property safetyGently, have no toxic side effect, meet the consumption concept that the mankind pursue safe and healthy ecological sustainability, its chemical constitution is simple, is easy toDegraded, belongs to environmentally friendly machine；Second, rhamnolipid has the ability of significant wetting and emulsified fat, to all kinds of vegetable fatThere is excellent emulsification with animal fat, can meet the needs of cleaning skin of human body and hair dirt completely；Third, mouseLee's glycolipid is pure biological agent, and production process is gentle, avoids chemical industry synthesis detergent production process high temperature high pressure catalyst bandThe high energy consumption high pollution problem come, it is more environment-friendly, and it is exceeded thoroughly to have prevented the dioxanes class harmful substance that side reaction is broughtTechnical barrier.It is well known that when obtaining its metabolite using microbial fermentation technology, suitable strain, fermentation conditionIt is real in production for years and advanced extraction and purification techniques are the necessary technology bases for obtaining high-quality metaboliteWe have accumulated the technology that a whole set of exclusive fermentation prepares rhamnolipid that summed up in trampling, and are provided for the quality of final productsAbundant and solid technical guarantee.
By implementing the specific content of the invention of the present invention, following technique effect can be reached.
（1）Rhamnolipid personal cleanliness articles for use provided by the invention, can have using the rhamnolipid of safe without toxic side effectThe dirt on cleaning skin of human body and hair is imitated, is especially difficult to the grease directly washed off with clear water, as of the invention personal clearThe core decontamination functional component of clean articles for use, instead of the use of chemical industry synthesis class detergent completely, ensure the same of cleaning effectWhen, avoid damage and stimulation of all kinds of chemical detergent human bodies to skin and hair.
（2）Rhamnolipid personal cleanliness articles for use provided by the invention, have remarkable all kinds of vegetable fat of emulsification and animalThe effect of grease, has thoroughly cleaning effect to body metabolism sebum, can thoroughly remove it is permanent be deposited in it is greasy in poreAnd dirt, emulsification is rapid, and foam is easily rinsed well by clear water, and it is not tight to wash rear skin.
（3）Rhamnolipid personal cleanliness articles for use provided by the invention using citric acid as acidity-basicity regulator, have safety,The characteristics of gentle so that the acid-base property of product close to skin surface natural acid-base status, so as to reduce the stimulation to skin, andAssign the skin after cleaning fine and smooth soft and smooth skin sense.
（4）Rhamnolipid personal cleanliness articles for use provided by the invention, it is deodorant tune to add a small amount of natural perfume material Peppermint Water,Ensure it is safe and non-toxic on the premise of, assign the fragrance of product pleasure, and refrigerant sensation so that consumer's body in useThe heart is happy.Industrial essence is avoided to endanger the cumulative toxicity of human body at the same time.
（5）Rhamnolipid personal cleanliness articles for use preparation process provided by the invention largely belongs to microorganism brewing process,It is the combination of technology and art, production process is environmentally protective, it is possible to achieve a complete sustainable Ecological Industry Chain, has notAppreciable social benefit and ecological benefits.
（6）Rhamnolipid personal cleanliness articles for use provided by the invention are realized similar to honey because it is concentrated type productAntiseptic principle, by controlling water content, improve osmotic pressure to reach antibacterial effect, so as to thoroughly avoid additionally adding variousWhat preservative was brought absorbs and cumulative toxicity risk through skin.
（7）Rhamnolipid personal cleanliness articles for use provided by the invention are because its component is simple, relative to third generation chemical industry synthesisIt is few then ten several in class articles for washing, for the product of at most tens kinds chemical compositions, greatly reduce depositing for skin allergy sourceBy up to a hundred person-times try out, the consumer to allergy of the present invention is not being found so far.
Embodiment
In the following, illustrating the present invention for embodiment, still, the present invention is not limited to following embodiments.
The raw material used for：Citric acid, natural perfume material, Peppermint Water, deionized water etc..This area can pass through the prior artObtain or prepare.
All raw and auxiliary materials, reagent and the instrument selected in the present invention, equipment are all well known in the art selection, but unlimitedThe implementation of the present invention is made, other some reagents well known in the art and equipment are applied both to the reality of implementation below of the present inventionApply, the rhamnolipid prepared especially with routine techniques fermentation known to this area also falls into protection scope of the present invention.
Embodiment one：A kind of preparation of rhamnolipid personal cleanliness articles for use
The preparation method of rhamnolipid personal cleanliness's articles for use, rhamnolipid personal cleanliness's articles for use component is according to weight part ratioMeter, specific preparation process are as follows：
（1）Use bacterium numbering will be new through above-mentioned non-perpendicular ferment tank culture for the pseudomonas aeruginosa of AB93066Fresh plant oil is converted into rhamnolipid, the mouse that the rhamnolipid content obtained after onestep extraction of going forward side by side, refined, concentration is 90-95%Lee's glycolipid condensed cream, according to weight part ratio meter, the rhamnolipid concentration lotion that 70-80 parts of accurate weighing is to be diluted.
（2）Accurate to weigh 0.1-0.3 parts of citric acids and 0.2-0.3 parts of Peppermint Waters, deionized water supplies 100 parts, obtains 200Gram deionized water solution it is spare.
（3）By step（2）Described in deionized water solution, add step（1）Described in 90%-95% rhamnolipidIn condensed cream, it is slowly stirred to abundant dissolving, it is product to refine after deaerating, and the content of rhamnolipid is 72-76% in product,Shelf life of products is not less than 1 year.
The rhamnolipid condensed cream of above-mentioned 90%-95% is obtained using following preparation method, by using fresh plant oilAs carbon source, using pseudomonas aeruginosa as fermenting microbe is fermented, extraction, refined and concentration form, comprise the following steps that：
（1）Spawn incubation：The pseudomonas aeruginosa parent species that a ring newly purifies are scraped, is inoculated in Shake flask medium and cultivates,Specific condition of culture is 35-38 DEG C of temperature, rotating speed 150-180rpm, and culture 40-60h obtains shake-flask seed.
（2）Fermentation：Above-mentioned shake-flask seed is inoculated into fermentation tank, inoculum concentration is the 5-10% of fermentation medium volume, is sent outFerment temperature control is at 35-38 DEG C, without from zymotic fluid bottom ventilation, avoiding the technical barrier of later stage foam overflow, more need notEthanol defoams, mixing speed 20-40rpm, and fermentation time is 5-6 days.In fermentation process, pH can be raised constantly, be utilized every 12hBetween zymotic fluid is recalled to pH6.5-6.8 by 55% citric acid solution, until fermentation ends, obtain yellow green zymotic fluid.
（3）Precipitated product：Citric acid is added in above-mentioned zymotic fluid, adjusting solution ph is 4.0-4.5, stands 10-30min, abandons supernatant, collects bottom sedimentation products, obtains rhamnolipid crude product, content 40-60%.
（4）It is refined：By above-mentioned steps（3）Rhamnolipid precipitation crude product further washes away free citric acid near with clear waterAfter neutrality, redissolve in the soda water of 1-2%, stir to thorough dissolving, pH value of solution and be adjusted between 7.0-7.5, rhamnolipid concentration10-20%, 80-90 DEG C is heated to by solution, and the active carbon particle for adding 3-5% carries out decoloration and deodorization to rhamnolipid crude product, gentlyStirring 1h is rubbed, standing treats that nature cools, with the above-mentioned solution of 100 mesh screens, pH value of solution is recalled to 4.0- again with citric acid4.5, sedimentation products are recycled, and wash precipitation 3 times with clear water, and precipitation is recalled into pH weakly acidic pH 6.8-7.2 with sodium bicarbonate, will be above-mentionedPrecipitation concentration is that rhamnolipid condensed cream content is 85-95%, and above-mentioned rhamnolipid is extracted with absolute ethyl alcohol under the conditions of -20 DEG CCondensed cream, extracts 3 times, merges above-mentioned ethanol solution and filter, and then nearly Rotary Evaporators recycling ethanol, acquisition contains rhamnolipidThe refined rhamnolipid condensed cream of 90-95%.
Above-mentioned steps（1）In, the shake-flask seed culture medium composition is as follows：10 ~ 20g/L of fresh plant oil;NaNO33~5g/L;K2HPO41.5~2g/L;KH2PO40.7~1g/L;MgSO40.3~0.5g/L；0.1 ~ 0.2g/L of yeast extract, surplus are water.
Above-mentioned steps（2）In, the fermentation medium composition is as follows：25 ~ 35g/L of fresh plant oil;NaNO33~5g/L;K2HPO41.5~2g/L;KH2PO40.7~1g/L;MgSO40.3~0.5g/L；0.05 ~ 0.1g/L of yeast extract, surplus are water.
Above-mentioned steps（5）（6）In, the fresh plant oil can be the peanut oil of fresh squeezing, walnut oil, olive oil,Apricot kernel oil, sesame oil, soybean oil, rapeseed oil, sunflower oil, maize germ oil, safflower seed oil etc., but it is not limited to above-mentioned vegetable oilFat.
Above-mentioned steps（2）In, the fermentation tank is not conventional upright gas-lifting type stirred fermentor, but is used non-perpendicularInclination tank body, and by the driving tank body rotation of tank base motor, tank base is equipped with fixed paddle, and add includingBut the customary secondary equipment such as the probe such as temperature common on fermentation tank, pH, dissolved oxygen and liquid level and corresponding control device is not limited to,Therefore it can realize that the stirring under the conditions of the slow-speed of revolution is ventilated and sufficient dissolved oxygen effect, save the cost of power consumption.
Embodiment two：A kind of formula of rhamnolipid personal cleanliness articles for use
The present invention provides a kind of rhamnolipid personal cleanliness articles for use, and it is the sandlwood of 90%-95% to count formula by weight50-95 parts of glycolipid condensed cream, 0.1-0.5 parts of citric acid, 0.1-0.5 parts of Peppermint Water, pH value 5.0-10.0, deionized water is supplied100 parts.
Further, count by weight, the present invention provides what a kind of rhamnolipid personal cleanliness articles for use were preferentially selected to match somebody with somebody70-80 parts of the rhamnolipid condensed cream of Fang Wei, 90%-95%, 0.1-0.3 parts of citric acid, 0.2-0.3 parts of Peppermint Water, pH value 6.0-8.0, deionized water supplies 100 parts.
Rhamnolipid personal cleanliness articles for use are formulated best composition：Count by weight, the rhamnolipid concentration of 90%-95%75 parts of cream, 0.1 part of citric acid, 0.2 part of Peppermint Water, pH value 6.5-7.0 deionized waters supply 100 parts.
Embodiment three：A kind of formula of rhamnolipid personal cleanliness articles for use
Count by weight, rhamnolipid personal cleanliness's articles for use formula is 1000 grams of rhamnolipid condensed cream, citric acid 2Gram, 2 grams of Peppermint Water, 196 grams of deionized water, after 2 grams of citric acids and 2 grams of Peppermint Waters are dissolved in 196 grams of water, will be above-mentionedMixed liquor is added in 1000 grams of rhamnolipid condensed creams, stands degassing after being sufficiently stirred dissolving or centrifugation degassing, detection are qualifiedIt is finished product afterwards.
Further, count by weight, rhamnolipid personal cleanliness's articles for use formula is 500 grams of rhamnolipid condensed cream, lemon2 grams of lemon acid, 2 grams of Peppermint Water, 196 grams of deionized water, after 2 grams of citric acids and 2 grams of Peppermint Waters are dissolved in 196 grams of water,Above-mentioned mixed liquor is added in 500 grams of rhamnolipid condensed creams, degassing or centrifugation degassing, detection are stood after being sufficiently stirred dissolvingIt is finished product after qualification.
Example IV：A kind of formula of rhamnolipid personal cleanliness articles for use
Antibacterial peptide hand sanitizer formula best composition is：Count by weight, rhamnolipid personal cleanliness's articles for use formula is mouse800 grams of Lee's glycolipid condensed cream, 1 gram of citric acid, 2 grams of Peppermint Water, 197 grams of deionized water are water-soluble by 1 gram of citric acid and 2 grams of peppermintsAfter solution is in 197 grams of water, above-mentioned mixed liquor is being added in 800 grams of rhamnolipid condensed creams, is being sufficiently stirred quiet after dissolvingDegassing or centrifugation degassing are put, is finished product after detection is qualified.
The rhamnolipid personal cleanliness articles for use obtained by above-mentioned preparation method are faint yellow sticky paste, such as honey thoroughlyIt is bright pure, it is personal cleanliness's articles for use that unique decontamination functional component is directly prepared by using rhamnolipid, by laboratory breastChanging experiment, verification goes oil removal, the effect of deep-pollution-removing, and skin sense exquisiteness soft and smooth, salubrious pleasant with thorough repeatedly.
Embodiment five：The evaluation of rhamnolipid personal cleanliness's articles for use clean effect
The rhamnolipid personal cleanliness's articles for use liquid prepared by embodiment one, embodiment two carries out washing tests.
（1）The clean effect method of rhamnolipid personal cleanliness's articles for use
0.5g rhamnolipid personal cleanliness's articles for use are added in beaker, 10g is added water to, shakes up, pure machine oil is added dropwise, are vibrated, dropIt is added to untill last drop machine oil do not dissolve, accurate recording rhamnolipid personal cleanliness articles for use dissolve the quality of pure machine oil, with noteThe size for recording quality characterizes the relative size of rhamnolipid personal cleanliness's articles for use dirt-removing power.
Table 1-1 detergency abilities contrast
Rhamnolipid personal cleanliness's articles for use clean effect test result indicates that, according to table 1-1, prepared by the present inventionRhamnolipid personal cleanliness articles for use there is significant clean effect, clean effect is than Fructus Gleditsiae Abnormalis extract, conventional toilet soaps and chemical industryThe cleaning effect of the synthesis compound detergent class facial cleanser of class improves very much, and emulsifies rapidly, and it is lasting that emulsion is stablized, especiallySuitable skin ingress of oil is more, or has a use for the crowd that makeup is accustomed to, and unfavorable crowd is that those bear a pretty, delicate skin and fuel-displaced fewInfant.
Embodiment six：Rhamnolipid personal cleanliness articles for use use the evaluation of rear skin sense
The in-service evaluation of cleaning supplies is there are certain subjectivity, we are using the in-service evaluation for listening to up to a hundred person-times extensivelyIn conjunction with product nucleus and design feature, by setting multinomial evaluation index, make as far as possible relatively comprehensive and rationalityEvaluate and quantify to give a mark, from table 1-2 can be seen that comprehensive performance it is classic be product of the present invention, and the feature of environmental protection of water is very wellIt cannot solve greasy primary demand, the environmental protection and decontamination of Fructus Gleditsiae Abnormalis extract are all good but lunging property is strong and uses process fiber cropsIt is tired.Perfumed soap is between natural Fructus Gleditsiae Abnormalis extract and chemical industry synthesis detergent, and there are complicated component, difficulty for chemical industry synthesis class detergentDegraded, the shortcomings that detergency is limited.
Table 1-2 is contrasted using rear sensory evaluation
It is the basic speciality of human nature to safe and environment-friendly and healthy pursuit, more classical product is simpler, therefore the present inventionProduct will have unlimited vitality in following personal cleanliness's articles for use field, and one become in mankind's daily life is requiredProduct.
Such as same arrow-leaved oleaster flowery odour, it is believed that fragrant but also it is believed that smelly, same color someone likes also someoneDo not like, therefore pass different judgements in the invention described above product odour and the aspect of color and luster two, the evaluation of consumer, we can be by notThe disconnected production technology level that improves to cause the color and luster of product and smell more perfect, provided for consumer can not be substituted safety,Environmentally friendly, clean, comfortable user experience.
In summary as it can be seen that it is an object of the invention to provide a kind of pure biological agent rhamnolipid personal cleansingProduct, using the rhanolipid as biosurfactant having no toxic side effect as unique core functional component, are aided with micro Peppermint Water tunePerfume processing, personal cleanliness's articles for use are prepared using the technological means such as fermentation, extraction, refined, concentration.Personal cleanliness's articles for use of preparation canFor the cleaning decontamination of skin surface and hair, cleaning effect is obvious, thoroughly goes oil removal, while available for oil-based cosmetic preparationCleaning makeup removing, improves makeup removal efficiency, and has certain bacteriostasis, due to being formulated extremely merely so wind skin allergyDanger falls below minimum, and simple in structure, degradable, Environmental compatibility is good, and it is clean soft salubrious to wash rear skin sense, not tight, non-scale,It is personal cleanliness's articles for use that a new generation is simple, green, environmentally friendly, healthy, it is excellent with can not be substituted in personal cleanliness's articles for use fieldGesture.
The present invention is all to follow all falling for change small known to this area and modification for concentration and simple component spiritEnter protection scope of the present invention, such as the including but not limited to above-mentioned various changes that can substitute classical formula, both fall within this hairBright protection domain, such as include but not limited to change product colour appearance, or a small amount of institute of addition by adding a small amount of pigmentThe effect of calling plant essence component, all behaviors disguised replacement of concept all are considered as malice and encroach on the present invention, therefore include but not limited toAbove-mentioned behavior both falls within protection scope of the present invention.