技术领域technical field
本发明涉及一种注射装置，具体地说，是涉及一种试剂连续定量注射装置。The present invention relates to an injection device, in particular to a continuous quantitative injection device for reagents.
背景技术Background technique
微流控技术是基于微机电加工（MEMS）的技术，芯片是通过维系加工将微通道、微电极、微泵、微阀、微储存槽、微检测模块和连接器等微功能模块集成在芯片材料上的微全分析系统。由于微流控芯片较常规化学生物分析仪要高效快捷、节约试剂和成本、高集成度和高精密度等优点，最近几年不仅受到了国内外专家的广泛研究，并且在生物和化学领域内的应用得到了迅猛发展。Microfluidic technology is based on micro-electromechanical processing (MEMS) technology. The chip integrates micro-functional modules such as micro-channels, micro-electrodes, micro-pumps, micro-valves, micro-storage tanks, micro-detection modules and connectors into the chip through maintenance processing. Micro-total analysis system on materials. Compared with conventional chemical and biological analyzers, microfluidic chips are more efficient and quicker, save reagents and costs, and have high integration and high precision. applications have developed rapidly.
微流体的定量注射操作是微流控芯片分析技术实现的核心，是微流控芯片实现试剂比例混合的前提。到目前为止，并没有一种专门针对于微流控芯片微通道中试剂连续定量的注射技术，传统的技术是利用机械式注射器或者滴定仪向微通道中注入定量的试剂，从而实现溶液的比例混合，该方法注射效率低，注射困难，同时密封性较差，注射时针头容易摆动而造成漏液或注入气泡。并且该方法的自动化程度较低，微流体试剂在通道中的混合无法实现直接简单控制，且微流体内的试剂并不能完全混合均匀。The quantitative injection operation of microfluidics is the core of microfluidic chip analysis technology, and it is the premise of microfluidic chip to achieve reagent ratio mixing. So far, there is no specific injection technology for the continuous quantification of reagents in the microchannels of microfluidic chips. The traditional technology is to use mechanical syringes or titrators to inject quantitative reagents into the microchannels to achieve the proportion of the solution. Mixing, the injection efficiency of this method is low, the injection is difficult, and the sealing is poor at the same time, and the needle is easy to swing during injection, causing liquid leakage or injecting air bubbles. Moreover, the degree of automation of this method is low, the mixing of microfluidic reagents in the channel cannot be directly and simply controlled, and the reagents in the microfluidic cannot be completely mixed evenly.
发明内容Contents of the invention
本发明为了解决现有微流体的定量注射利用机械式注射器或者滴定仪注射效率低的技术问题，提出了一种试剂连续定量注射装置，可以解决上述问题。In order to solve the technical problem of low injection efficiency of existing microfluidic quantitative injection using mechanical syringes or titrators, the present invention proposes a continuous quantitative injection device for reagents, which can solve the above problems.
为了解决上述技术问题，本发明采用以下技术方案予以实现：In order to solve the above-mentioned technical problems, the present invention adopts the following technical solutions to achieve:
一种试剂连续定量注射装置，包括动力机构、至少一个注射器、以及支撑连接机构，所述支撑连接机构包括上固定板、下固定板、和转接板，所述动力机构包括电机和动力输出轴，所述注射器包括针头、针筒、活塞杆、以及固设于所述活塞杆端部的活塞，所述上固定板通过支撑杆固定在微流控芯片的上方，所述下固定板位于所述上固定板的下方，且通过连接杆与所述上固定板固定连接，所述转接板位于所述上固定板和下固定板之间，且所述转接板上开有用于容所述支撑杆穿过的导向孔，所述电机固设于所述上固定板上，所述上固定板、转接板、和下固定板同轴设置，且中心分别开有中心孔，所述动力输出轴依次穿过所述上固定板、转接板、和下固定板的中心孔，所述动力输出轴的外表面具有外螺纹，转接板的中心孔的内表面具有与所述外螺纹相匹配的内螺纹，所述针筒与所述下固定板固定连接，所述活塞杆与所述转接板固定连接，所述电机与上位机连接。A reagent continuous quantitative injection device, comprising a power mechanism, at least one syringe, and a support connection mechanism, the support connection mechanism includes an upper fixing plate, a lower fixing plate, and an adapter plate, and the power mechanism includes a motor and a power output shaft , the syringe includes a needle, a syringe, a piston rod, and a piston fixed at the end of the piston rod, the upper fixing plate is fixed above the microfluidic chip through a support rod, and the lower fixing plate is located on the Below the upper fixing plate, and fixedly connected with the upper fixing plate through a connecting rod, the adapter plate is located between the upper fixing plate and the lower fixing plate, and the adapter plate is opened for accommodating The guide hole through which the support rod passes, the motor is fixed on the upper fixed plate, the upper fixed plate, the adapter plate, and the lower fixed plate are coaxially arranged, and the center is respectively opened with a central hole, the The power output shaft passes through the central hole of the upper fixing plate, the adapter plate, and the lower fixing plate in turn, the outer surface of the power output shaft has external threads, and the inner surface of the central hole of the adapter plate has a The thread matches the internal thread, the syringe is fixedly connected to the lower fixing plate, the piston rod is fixedly connected to the adapter plate, and the motor is connected to the upper computer.
进一步的，在所述上固定板的下表面和/或所述下固定板的上表面上分别固设有接近开关，所述接近开关与所述上位机连接。Further, proximity switches are respectively fixed on the lower surface of the upper fixing plate and/or the upper surface of the lower fixing plate, and the proximity switches are connected to the host computer.
进一步的，所述电机为微型步进电机。Further, the motor is a micro stepping motor.
进一步的，在微流控芯片的上表面上设有保护套筒，所述动力输出轴伸入至所述保护套筒中，且所述动力输出轴的端部与微流控制芯片之间具有间隙。Further, a protective sleeve is provided on the upper surface of the microfluidic chip, the power output shaft extends into the protective sleeve, and there is a gap between the end of the power output shaft and the microfluidic control chip. gap.
进一步的，所述针头位于微流控制芯片上微通道的上方，且所述针头伸入至所述微通道的槽内。Further, the needle is located above the microchannel on the microfluidic control chip, and the needle protrudes into the groove of the microchannel.
与现有技术相比，本发明的优点和积极效果是：本发明的试剂连续定量注射装置，通过设置动力机构以及至少一个注射器，动力机构接受上位机的控制，驱动注射器完成注射，能够实现连续自动注射，而且动力机构可以同时驱动一个及以上注射器，进一步提高了注射效率。Compared with the prior art, the advantages and positive effects of the present invention are: the reagent continuous quantitative injection device of the present invention, by setting the power mechanism and at least one syringe, the power mechanism accepts the control of the host computer, drives the syringe to complete the injection, and can realize continuous injection. Automatic injection, and the power mechanism can drive one or more syringes at the same time, which further improves the injection efficiency.
结合附图阅读本发明实施方式的详细描述后，本发明的其他特点和优点将变得更加清楚。Other features and advantages of the present invention will become more apparent after reading the detailed description of the embodiments of the present invention in conjunction with the accompanying drawings.
附图说明Description of drawings
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present invention. Those skilled in the art can also obtain other drawings based on these drawings without creative work.
图1是本发明所提出的试剂连续定量注射装置的一种实施例结构示意图；Fig. 1 is a kind of structural representation of the embodiment of the reagent continuous quantitative injection device proposed by the present invention;
图2是本发明所提出的试剂连续定量注射装置的一种实施例的立体图。Fig. 2 is a perspective view of an embodiment of the reagent continuous quantitative injection device proposed by the present invention.
具体实施方式Detailed ways
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
实施例一，本实施例提出了一种试剂连续定量注射装置，如图1所示，包括动力机构10、至少一个注射器20、以及支撑连接机构30，所述支撑连接机构30包括上固定板301、下固定板302、和转接板303，所述动力机构10包括电机101和动力输出轴102，所述注射器20包括针头201、针筒202、活塞杆203、以及固设于所述活塞杆203端部的活塞204，所述上固定板301通过支撑杆401固定在微流控芯片60的上方，所述下固定板302位于所述上固定板301的下方，且通过连接杆402与所述上固定板301固定连接，所述转接板303位于所述上固定板301和下固定板302之间，且所述转接板303上开有用于容所述支撑杆401穿过的导向孔303b，所述电机101固设于所述上固定板301上，所述上固定板301、转接板303、和下固定板302同轴设置，且中心分别开有中心孔，所述动力输出轴102依次穿过所述上固定板301、转接板303、和下固定板302的中心孔，所述动力输出轴102的外表面具有外螺纹，转接板303的中心孔的内表面具有与所述外螺纹相匹配的内螺纹，所述针筒202与所述下固定板302固定连接，所述活塞杆203与所述转接板303固定连接，所述电机101与上位机连接。本实施例的试剂连续定量注射装置，电机101驱动动力输出轴102转动，在转接板303的内螺纹与动力输出轴102的外螺纹的配合下，带动转接板303上下运动，进而驱动活塞杆203在针筒202内上下运动完成注射动作，因此转接板303在电机101的驱动下可以推动活塞杆203做往复运动，从而完成连续注射动作，注射过程中上位机通过控制电机101的转速，从而控制试剂注射速度。本实施例的试剂连续定量注射装置，通过设置动力机构以及至少一个注射器，动力机构接受上位机的控制，驱动注射器完成注射，能够实现连续自动注射，而且动力机构可以同时驱动一个及以上注射器，进一步提高了注射效率。Embodiment 1. This embodiment proposes a reagent continuous quantitative injection device, as shown in FIG. , a lower fixing plate 302, and an adapter plate 303, the power mechanism 10 includes a motor 101 and a power output shaft 102, and the syringe 20 includes a needle 201, a syringe 202, a piston rod 203, and is fixed on the piston rod The piston 204 at the end of 203, the upper fixing plate 301 is fixed above the microfluidic chip 60 through the support rod 401, the lower fixing plate 302 is located below the upper fixing plate 301, and is connected with the connecting rod 402 The upper fixing plate 301 is fixedly connected, the adapter plate 303 is located between the upper fixing plate 301 and the lower fixing plate 302, and the adapter plate 303 is provided with a guide for allowing the support rod 401 to pass through hole 303b, the motor 101 is fixed on the upper fixing plate 301, the upper fixing plate 301, the adapter plate 303, and the lower fixing plate 302 are coaxially arranged, and the center is respectively opened with a center hole, the power The output shaft 102 passes through the central hole of the upper fixed plate 301, the adapter plate 303, and the lower fixed plate 302 in turn, the outer surface of the power output shaft 102 has external threads, and the inner surface of the central hole of the adapter plate 303 It has an internal thread matching the external thread, the syringe 202 is fixedly connected to the lower fixing plate 302, the piston rod 203 is fixedly connected to the adapter plate 303, and the motor 101 is connected to the host computer . In the reagent continuous quantitative injection device of this embodiment, the motor 101 drives the power output shaft 102 to rotate, and under the cooperation of the internal thread of the adapter plate 303 and the external thread of the power output shaft 102, the adapter plate 303 is driven to move up and down, thereby driving the piston The rod 203 moves up and down in the syringe 202 to complete the injection action, so the adapter plate 303 can push the piston rod 203 to reciprocate under the drive of the motor 101, thereby completing the continuous injection action. During the injection process, the upper computer controls the speed of the motor 101 , so as to control the reagent injection speed. The reagent continuous quantitative injection device of this embodiment, by setting a power mechanism and at least one syringe, the power mechanism accepts the control of the host computer, drives the syringe to complete the injection, can realize continuous automatic injection, and the power mechanism can drive one or more syringes at the same time, further Improved injection efficiency.
上位机的控制系统中含有流量调节按钮，定时注射按钮，流程设置以及时间调节按钮等，如果实验中要求不同比例的试剂混合，可以通过上位机设置流程调解步进电动机的转速控制注射速度，并定时开关对应微阀50的开启和关闭来按照混合比对试剂进样的先后步骤的预设，而且我们还可以通过上位机设置流程，从而完成试剂的注射，试剂的抽取以及仪器的清洗等多系列步骤。The control system of the upper computer includes flow adjustment buttons, timing injection buttons, process setting and time adjustment buttons, etc. If different proportions of reagents are required to be mixed in the experiment, the speed of the stepping motor can be adjusted to control the injection speed through the upper computer setting process, and The timing switch corresponds to the opening and closing of the microvalve 50 to preset the steps of reagent injection according to the mixing ratio, and we can also set the process through the host computer to complete reagent injection, reagent extraction, and instrument cleaning. series of steps.
优选在本实施例中，在所述上固定板301的下表面和/或所述下固定板302的上表面上分别固设有接近开关304，所述接近开关304与所述上位机连接。接近开关用于检测转接板303的位置，当防止转接板303运动至上固定板301或者下固定板302时产生碰撞而损坏设备，当转接板303向上运动至限定位置时，设置于上固定板301上的接近开关可以检测到转接板303的接近信号，并产生检测信号发送至上位机，由上位机控制电机101反转，进而控制转接板303反向运动，或者控制电机101停止，使其与上固定板301保持在安全距离范围内，同样道理的，当转接板303向下运动至限定位置时，设置于下固定板302上的接近开关可以检测到转接板303的接近信号，并产生检测信号发送至上位机，由上位机控制电机101反转，进而控制转接板303反向运动，或者控制电机101停止，使其与下固定板302保持在安全距离范围内。Preferably, in this embodiment, a proximity switch 304 is respectively fixed on the lower surface of the upper fixing plate 301 and/or the upper surface of the lower fixing plate 302, and the proximity switch 304 is connected to the host computer. The proximity switch is used to detect the position of the adapter plate 303. When the adapter plate 303 is prevented from moving to the upper fixed plate 301 or the lower fixed plate 302, it will collide and damage the equipment. When the adapter plate 303 moves upward to a limited position, it is set on the upper The proximity switch on the fixed plate 301 can detect the proximity signal of the adapter plate 303, and generate a detection signal and send it to the host computer, and the host computer controls the reverse rotation of the motor 101, and then controls the reverse movement of the adapter plate 303, or controls the motor 101 Stop to keep it within a safe distance from the upper fixing plate 301. For the same reason, when the adapter plate 303 moves down to a limited position, the proximity switch arranged on the lower fixing plate 302 can detect the adapter plate 303 The proximity signal is generated, and a detection signal is sent to the upper computer, and the upper computer controls the motor 101 to reverse, and then controls the reverse movement of the adapter plate 303, or controls the motor 101 to stop, so that it is kept at a safe distance from the lower fixing plate 302 Inside.
优选在本实施例中，所述电机101为微型步进电机，其步长小，控制更加精准。Preferably, in this embodiment, the motor 101 is a micro stepping motor with a small step size and more precise control.
由于电机101带动动力输出轴102转动，进而实现驱动活塞杆203动作，为了防止动力输出轴102在转动时其端部损坏到其他器件，或者防止误伤到实验人员，在微流控芯片60的上表面上设有保护套筒601，所述动力输出轴102伸入至所述保护套筒601中，且所述动力输出轴102的端部与微流控制芯片60之间具有间隙。Since the motor 101 drives the power output shaft 102 to rotate, and then realizes the action of driving the piston rod 203, in order to prevent the end of the power output shaft 102 from damaging other devices when rotating, or to prevent accidental injury to the experimenter, the microfluidic chip 60 A protective sleeve 601 is provided on the surface, the power output shaft 102 protrudes into the protective sleeve 601 , and there is a gap between the end of the power output shaft 102 and the microfluidic control chip 60 .
为了解决目前注射时产生气泡的问题，本实施例中所述针头201位于微流控制芯片60上微通道602的上方，且所述针头201伸入至所述微通道602的槽内。因此针头201与微通道602紧密连接，形成密封区域，以防气泡的产生。In order to solve the current problem of air bubbles during injection, the needle 201 in this embodiment is located above the microchannel 602 on the microfluidic control chip 60 , and the needle 201 extends into the groove of the microchannel 602 . Therefore, the needle 201 is tightly connected with the microchannel 602 to form a sealed area to prevent the generation of air bubbles.
当然，上述说明并非是对本发明的限制，本发明也并不仅限于上述举例，本技术领域的普通技术人员在本发明的实质范围内所做出的变化、改型、添加或替换，也应属于本发明的保护范围。Of course, the above descriptions are not intended to limit the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by those skilled in the art within the scope of the present invention shall also belong to protection scope of the present invention.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510196205.6ACN104826542A (en) | 2015-04-23 | 2015-04-23 | Reagent continuous quantitative injection device |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510196205.6ACN104826542A (en) | 2015-04-23 | 2015-04-23 | Reagent continuous quantitative injection device |
| Publication Number | Publication Date |
|---|---|
| CN104826542Atrue CN104826542A (en) | 2015-08-12 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510196205.6APendingCN104826542A (en) | 2015-04-23 | 2015-04-23 | Reagent continuous quantitative injection device |
| Country | Link |
|---|---|
| CN (1) | CN104826542A (en) |
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| CN109602404A (en)* | 2018-12-21 | 2019-04-12 | 上海百铭信息科技有限公司 | A smart emergency watch |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication | Application publication date:20150812 |