技术领域technical field
本发明涉及利用医学影像技术研究模式植物拟南芥盐分处理机制的方法，属于植物生理学技术领域。The invention relates to a method for studying the salt processing mechanism of the model plant Arabidopsis thaliana by using medical imaging technology, and belongs to the technical field of plant physiology.
背景技术Background technique
拟南芥（Arabidopsis thaliana）是被子植物门、双子叶植物纲、白花菜目、十字花科、大蒜芥族、拟南芥属的二年生草本，主要分布在西南亚，中亚，地中海，北极、北美和南美的高山等。我国内蒙、新疆、陕西、甘肃、西藏、山东、江苏、安徽、湖北、四川、云南等省区均有发现。拟南芥的优点是植株小、结子多。拟南芥的基因组是目前已知植物基因组中最小的。拟南芥是自花受粉植物，基因高度纯合，用理化因素处理突变率很高，容易获得各种代谢功能的缺陷型。拟南芥是进行遗传学和植物生理学研究的好材料，已被广泛应用于多种实验中。Arabidopsis thaliana is a biennial herb belonging to the phylum Angiosperms, Dicotyledoneae, Brassicaceae, Brassicaceae, Garlic family, and Arabidopsis thaliana. It is mainly distributed in Southwest Asia, Central Asia, the Mediterranean, the Arctic, Mountains in North and South America, etc. It has been found in Inner Mongolia, Xinjiang, Shaanxi, Gansu, Tibet, Shandong, Jiangsu, Anhui, Hubei, Sichuan, Yunnan and other provinces and regions. The advantage of Arabidopsis is that the plants are small and have many knots. The Arabidopsis genome is the smallest known plant genome. Arabidopsis is a self-pollinating plant with high gene homozygosity. It has a high mutation rate when treated with physical and chemical factors, and it is easy to obtain defective types of various metabolic functions. Arabidopsis is a good material for genetics and plant physiology research, and has been widely used in various experiments.
焰色反应，也称作焰色测试及焰色试验，是某些金属或它们的化合物在无色火焰中灼烧时使火焰呈现特征的颜色的反应。其原理是每种元素都有其个别的光谱。样本通常是粉或小块的形式。以一条清洁且对化学惰性的金属线，例如铂或镍铬合金盛载样本，再放到无光焰蓝色火焰中。在化学上，常用来测试某种金属是否存在在于化合物。同时利用焰色反应，人们在在烟花中有意识地加入特定金属元素，使焰火更加绚丽多彩。焰色反应是物理变化。它并未生成新物质，焰色反应是物质原子内部电子能级的改变，通俗的说是原子中的电子能量的变化，不涉及物质结构和化学性质的改变。焰色反应是某些金属或它们的挥发性化合物在无色火焰中灼烧时使火焰呈现特征的颜色的反应。有些金属或它们的化合物在灼烧时能使火焰呈特殊颜色。Flame reaction, also known as flame test and flame test, is the reaction of certain metals or their compounds to make the flame present the characteristic color when burning in a colorless flame. The principle is that each element has its own individual spectrum. Samples are usually in the form of powders or small pieces. A clean, chemically inert metal wire, such as platinum or nichrome, holds the sample and places it in a flameless blue flame. In chemistry, it is often used to test whether a certain metal exists in a compound. At the same time, using the flame reaction, people consciously add specific metal elements to the fireworks to make the fireworks more colorful. Flame reaction is a physical change. It does not generate new substances. The flame reaction is the change of the electronic energy level inside the material atom. Generally speaking, it is the change of the electron energy in the atom, which does not involve the change of the structure and chemical properties of the material. Flame reaction is the reaction of certain metals or their volatile compounds to give the flame its characteristic color when burned in a colorless flame. Some metals or their compounds can give the flame a special color when burning.
微波炉是利用食物在微波场中吸收微波能量而使自身加热的烹饪器具。在微波炉微波发生器产生的微波在微波炉腔建立起微波电场，并采取一定的措施使这一微波电场在炉腔中尽量均匀分布，将食物放入该微波电场中，由控制中心控制其烹饪时间和微波电场强度，来进行各种各样的烹饪过程。微波是一种高频率的电磁波，其本身并不产生热，在宇宙、自然界中到处都有微波，但存在自然界的微波，因为分散不集中，故不能加热食品。微波炉乃是利用其内部的磁控管，将电能转变成微波，以2450MHZ的振荡频率穿透食物，当微波被食物吸收时，食物内之极性分子如水、脂肪、蛋白质、糖等即被吸引以每秒钟24.5亿次的速度快速振荡，这种震荡的宏观表现就是食物被加热了，而且它使整个物体受热均匀，升温速度也快。所以，微波加热的原理可以简单概括为：当微波辐射到食品上时，食品中总是含有一定量的水分，而水是由极性分子的正负电荷中心，即使在外电场不存在时也是不重合的组成的，这种极性分子的取向将随微波场而变动。由于食品中水的极性分子的这种运动。以及相邻分子间的相互作用，产生了类似摩擦的现象，使水温升高，因此，食品的温度也就上升了。但是，在日常使用微波炉的过程中，忌用金属器皿，原因是微波炉在加热时，放入炉内的铁、铝、不锈钢、搪瓷等器皿，金属器皿会产生电火花并燃烧。A microwave oven is a cooking appliance that uses food to absorb microwave energy in a microwave field to heat itself. The microwave generated by the microwave generator in the microwave oven establishes a microwave electric field in the cavity of the microwave oven, and certain measures are taken to make the microwave electric field evenly distributed in the cavity as much as possible, and the food is put into the microwave electric field, and the cooking time is controlled by the control center and microwave electric field strength to carry out various cooking processes. Microwave is a kind of high-frequency electromagnetic wave, which does not generate heat by itself. There are microwaves everywhere in the universe and nature, but there are microwaves in nature. Because they are scattered and not concentrated, they cannot heat food. The microwave oven uses its internal magnetron to convert electrical energy into microwaves, which penetrate food at an oscillation frequency of 2450MHZ. When microwaves are absorbed by food, polar molecules such as water, fat, protein, sugar, etc. in the food are attracted Oscillating rapidly at a rate of 2.45 billion times per second, the macroscopic manifestation of this oscillation is that the food is heated, and it makes the whole object heated evenly, and the heating speed is also fast. Therefore, the principle of microwave heating can be simply summarized as follows: when microwaves radiate to food, there is always a certain amount of water in the food, and water is the center of positive and negative charges of polar molecules, even when the external electric field does not exist. Composed of overlapping, the orientation of this polar molecule will change with the microwave field. Due to this movement of polar molecules of water in food. And the interaction between adjacent molecules produces a phenomenon similar to friction, which makes the water temperature rise, so the temperature of the food also rises. However, in the process of daily use of microwave ovens, metal utensils should not be used. The reason is that when the microwave oven is heated, iron, aluminum, stainless steel, enamel and other utensils placed in the furnace will generate electric sparks and burn.
在利用拟南芥研究模式植物盐分处理机制时，由于盐分分子极小，人们需要借助非常复杂的手段才能得出一定的结果，效果不是太理想。因此如何研究出一种可以直观呈现不同盐分在拟南芥植株体内运行及分布的方法成为急需解决的一大难题，所以在培养液中人为加入纳米金属颗粒，使之随着根对水分的吸收而到达不同的部位事先处理拟南芥植株，然后利用金属在微波处理时会燃烧，而且不同金属燃烧的颜色反应不同，接着利用光电倍增管将颜色信号扩增，由电脑根据颜色光谱信息得出拟南芥不同部位存在何种金属颗粒，从而可以直观地研究植物盐分处理机制，发明一种利用医学影像技术研究模式植物拟南芥盐分处理机制的方法是必要的。When using Arabidopsis to study the salt processing mechanism of model plants, because the salt molecules are extremely small, people need to use very complicated means to get certain results, and the effect is not ideal. Therefore, how to develop a method that can visually display the operation and distribution of different salts in Arabidopsis plants has become a major problem that needs to be solved urgently. Therefore, nano-metal particles are artificially added to the culture medium to make it follow the absorption of water by the roots. Arriving at different parts to treat Arabidopsis plants in advance, and then use metals to burn during microwave treatment, and the color reactions of different metals are different when burning, and then use photomultiplier tubes to amplify the color signals, which are obtained by the computer based on the color spectrum information. What kind of metal particles exist in different parts of Arabidopsis thaliana, so that the mechanism of plant salt processing can be intuitively studied. It is necessary to develop a method of using medical imaging technology to study the salt processing mechanism of the model plant Arabidopsis thaliana.
发明内容Contents of the invention
为了克服现实研究过程中不能直观呈现不同盐分在拟南芥植株体内运行及分布的难题，本发明提供了利用医学影像技术研究模式植物拟南芥盐分处理机制的方法，该种利用医学影像技术研究模式植物拟南芥盐分处理机制的方法，首先在培养液中人为加入直径为10-100纳米的纳米金属颗粒，使之随着根对水分的吸收而到达不同的部位，事先处理拟南芥植株，然后利用金属在微波处理时会燃烧，而且不同金属燃烧的颜色反应不同，接着利用光电倍增管将颜色信号扩增，由电脑根据颜色光谱信息得出拟南芥不同部位存在何种金属颗粒，从而达到直观地研究模式植物盐分处理机制的目的。In order to overcome the problem of inability to visualize the operation and distribution of different salts in Arabidopsis plants in the actual research process, the present invention provides a method for studying the salt processing mechanism of the model plant Arabidopsis thaliana by using medical imaging technology. The method of the salt treatment mechanism of the model plant Arabidopsis thaliana, first artificially add nano-metal particles with a diameter of 10-100 nanometers in the culture medium, so that they can reach different parts with the absorption of water by the roots, and treat the Arabidopsis plants in advance , and then use metals to burn during microwave treatment, and different metals have different color reactions when they burn, and then use photomultiplier tubes to amplify the color signals, and use the computer to obtain what kind of metal particles exist in different parts of Arabidopsis based on the color spectrum information. So as to achieve the purpose of intuitively studying the mechanism of salinity processing in model plants.
本发明解决其技术问题所采用的技术方案是：The technical solution adopted by the present invention to solve its technical problems is:
本发明利用医学影像技术研究模式植物拟南芥盐分处理机制的方法是根据日常生活中，医学影像技术已经非常成熟，对于生物体所能呈现出来的图形的分析技术也相当完善，故本发明设计由拟南芥植株栽培、纳米金属颗粒摄入和处理装置三部分构成，以便根据所得结果直管推测出拟南芥盐分的处理机制。The method of the present invention to study the salt processing mechanism of the model plant Arabidopsis thaliana is based on the fact that in daily life, the medical imaging technology is very mature, and the analysis technology for the graphics that the organism can present is also quite perfect, so the present invention designs It consists of three parts: Arabidopsis plant cultivation, metal nanoparticle intake and processing device, so as to directly deduce the processing mechanism of Arabidopsis salinity based on the obtained results.
所述的拟南芥植株栽培，用于获得实验所需要的拟南芥植株。The cultivation of the Arabidopsis plants is used to obtain the Arabidopsis plants required for the experiment.
所述的纳米金属颗粒摄入，是根据研究目的，选择需要研究的盐分，采用公知的技术，制备或购买市售的该种直径为10-100纳米的纳米金属颗粒，比如研究铁盐，则加入直径为10-100纳米的纳米铁颗粒。然后，将纳米金属颗粒混入拟南芥培养液，使纳米金属颗粒随水分由根吸收进拟南芥植物体内。The intake of nano metal particles is to select the salt to be studied according to the purpose of the research, and to prepare or purchase commercially available nano metal particles with a diameter of 10-100 nanometers, for example, to study iron salts, by using known techniques. Add nano-iron particles with a diameter of 10-100 nm. Then, the nano-metal particles are mixed into the Arabidopsis culture solution, so that the nano-metal particles are absorbed into the Arabidopsis plant from the root along with the water.
所述的处理装置由微波器壳1、数据处理电脑2、燃烧控制器3、微波处理舱4、微波发生器5、微波处理舱门6、拟南芥放置转盘7、电源开关8、电压控制旋钮9、模式调节旋钮10、数据显示板11、微波释放板12、壳顶透气孔13、微波器电源线14、微波器电源插头15、微波处理舱门把手16、光电倍增管群17、壳顶18、光子探测器19、数据传输线20、电脑底座21、电脑电源线22、电脑电源插头23组成。微波器壳1，由涂覆非磁性材料的金属板制成，长方体形，长度为50-100厘米，宽度为30-60厘米，高度为40-80厘米，内有燃烧控制器3、微波处理舱4和光谱采集器19。数据处理电脑2、光子探测器19、数据传输线20、电脑底座21、电脑电源线22和电脑电源插头23组成公知的光学多道分析仪OMA (Optical Multi-channelAnalyzer)。光子探测器19结构原理同公知的光子探测器(CCD)，主要组成部件为光电倍增管群17，用于信息采集,处理，并将处理后的图像信息由数据传输线20传输给数据处理电脑2。数据处理电脑2则进一步处理并存储相关信息，供使用者选择使用。光学多道分析仪OMA的电能由电脑电源线22和电脑电源插头23从外接电源插座引入。燃烧控制器3包括微波发生器5、电源开关8、电压控制旋钮9、模式调节旋钮10、数据显示板11、微波释放板12、微波器电源线14、微波器电源插头15。微波发生器5同公知微波炉的磁控管、波导和电源变压器：电源变压器是给磁控管提供电压的部件；磁控管实际上是一个真空管金属管，由它产生和发射微波直流电能转换成微波震荡输出；波导则是将磁控管产生的微波功率传输到微波处理舱4，以加热拟南芥体内的纳米金属颗粒使之燃烧发光。电源开关8控制微波器电源线14由微波器电源插头15从外接插座上引过来的电能。电压控制旋钮9同公知微波炉的电压控制旋钮，控制燃烧控制器3的电压，需要高电压则调到高电压处，需要低电压则调到低电压处。模式调节旋钮10同公知微波炉的时间控制旋钮，可以根据拟南芥植株的大小选择不同的刻度，主要是作用时间。数据显示板11同公知的液晶显示屏，可以显示时间的变化及电压的高低。微波释放板12是直径为10-15厘米的圆形具孔区域，位于微波处理舱4与微波发生器5相连的侧壁上，使微波发生器5产生的微波均匀地释放到微波处理舱4中。微波处理舱4：也称谐振腔，它是处理拟南芥植株的地方，经波导管输入炉腔内的微波在腔壁内来回反射，每次传播都穿过和经过拟南芥植株。在设计时，通常使微波处理舱4的边长为1/2微波导波波长的倍数，这样使拟南芥植株在被加热时，舱内能保持谐振，谐振范围适当变宽。在微波处理舱4的顶部开有2个壳顶透气孔13。微波处理舱门6的作用是便于取放拟南芥植株及观察处理时的情形，微波处理舱门6又是构成微波处理舱4的前壁，它是防止微波泄露的一道关卡。拟南芥放置转盘7是安装在微波处理舱4的底部，离炉底有的高度为1-1.5厘米，由一只以5-6转/分钟转速的小马达带动。The processing device consists of a microwave shell 1, a data processing computer 2, a combustion controller 3, a microwave processing cabin 4, a microwave generator 5, a microwave processing cabin door 6, an Arabidopsis placing turntable 7, a power switch 8, and a voltage control Knob 9, mode adjustment knob 10, data display board 11, microwave release board 12, shell top air hole 13, microwave power cord 14, microwave power plug 15, microwave processing hatch handle 16, photomultiplier tube group 17, shell A top 18, a photon detector 19, a data transmission line 20, a computer base 21, a computer power cord 22, and a computer power plug 23 are formed. Microwave oven shell 1, made of metal plate coated with non-magnetic material, cuboid shape, length 50-100 cm, width 30-60 cm, height 40-80 cm, combustion controller 3, microwave treatment cabin 4 and spectrum collector 19. The data processing computer 2, the photon detector 19, the data transmission line 20, the computer base 21, the computer power cord 22 and the computer power plug 23 form a known optical multi-channel analyzer OMA (Optical Multi-channel Analyzer). The structural principle of the photon detector 19 is the same as that of the known photon detector (CCD). The main component is the photomultiplier tube group 17, which is used for information collection and processing, and the processed image information is transmitted to the data processing computer 2 by the data transmission line 20 . The data processing computer 2 further processes and stores relevant information for users to choose and use. The electrical energy of the optical multi-channel analyzer OMA is introduced from an external power socket by a computer power cord 22 and a computer power plug 23 . The combustion controller 3 includes a microwave generator 5, a power switch 8, a voltage control knob 9, a mode adjustment knob 10, a data display board 11, a microwave release board 12, a microwave power cord 14, and a microwave power plug 15. The microwave generator 5 is the same as the magnetron, waveguide and power transformer of the known microwave oven: the power transformer is the part that provides voltage to the magnetron; The microwave oscillates output; the waveguide transmits the microwave power generated by the magnetron to the microwave treatment chamber 4, so as to heat the nano metal particles in the Arabidopsis to burn and emit light. The power switch 8 controls the electric energy that the microwave power supply line 14 draws from the external socket by the microwave power plug 15 . Voltage control knob 9 controls the voltage of combustion controller 3 with the voltage control knob of known microwave oven, needs high voltage then is transferred to high voltage place, needs low voltage then is transferred to low voltage place. The mode adjustment knob 10 is the same as the time control knob of the known microwave oven, and different scales can be selected according to the size of the Arabidopsis plant, mainly the action time. The data display panel 11 is the same as the known liquid crystal display, which can display the change of time and the level of voltage. The microwave release plate 12 is a circular perforated area with a diameter of 10-15 centimeters, located on the side wall where the microwave treatment cabin 4 is connected to the microwave generator 5, so that the microwaves generated by the microwave generator 5 are evenly released to the microwave treatment cabin 4 middle. Microwave processing cabin 4: also called resonant cavity, it is the place where Arabidopsis plants are processed. The microwave input into the furnace cavity through the waveguide is reflected back and forth in the cavity wall, passing through and passing through the Arabidopsis plants every time. When designing, the side length of the microwave processing cabin 4 is usually a multiple of the wavelength of 1/2 microwave guide wave, so that when the Arabidopsis plant is heated, the cabin can maintain resonance, and the resonance range is appropriately widened. On the top of the microwave processing cabin 4, there are two air holes 13 on the top of the shell. The effect of microwave treatment cabin door 6 is to be convenient to pick and place Arabidopsis plant and observe the situation during processing, and microwave treatment cabin door 6 is the front wall that constitutes microwave treatment cabin 4 again, and it is a checkpoint that prevents microwave from leaking. The Arabidopsis thaliana places the turntable 7 and is installed on the bottom of the microwave treatment cabin 4. The height that is 1-1.5 centimeters away from the bottom of the furnace is driven by a small motor with 5-6 revs/min.
本发明的有益效果为，利用医学影像技术研究模式植物拟南芥盐分处理机制的方法，首先在培养液中人为加入纳米金属颗粒，使之随着根对水分的吸收而到达不同的部位事先处理拟南芥植株，然后利用金属在微波处理时会燃烧，而且不同金属燃烧的颜色反应不同，接着利用光电倍增管将颜色信号扩增，由电脑根据颜色光谱信息得出拟南芥不同部位存在何种金属颗粒，从而达到可以直观地研究植物盐分处理机制的目的。利用医学影像技术研究模式植物拟南芥盐分处理机制的方法所用装置制作简单，可操作性强，成本低廉，效果明显。The beneficial effect of the present invention is that the method of using medical imaging technology to study the salt treatment mechanism of the model plant Arabidopsis thaliana first artificially adds nano-metal particles into the culture solution so that it can reach different parts with the absorption of water by the roots. Arabidopsis plants, and then use metals to burn when microwaved, and the color reactions of different metals are different, and then use photomultiplier tubes to amplify the color signals, and use the computer to obtain what is present in different parts of Arabidopsis based on the color spectrum information. metal particles, so as to achieve the purpose of intuitively studying the mechanism of plant salt processing. The device used in the method for studying the salt processing mechanism of the model plant Arabidopsis thaliana by using the medical imaging technology is simple in manufacture, strong in operability, low in cost and obvious in effect.
附图说明Description of drawings
下面结合附图对本发明进一步说明。The present invention will be further described below in conjunction with the accompanying drawings.
图1为本发明利用医学影像技术研究模式植物拟南芥盐分处理机制的方法的处理装置整体结构示意图。Fig. 1 is a schematic diagram of the overall structure of the processing device of the method for studying the salinity processing mechanism of the model plant Arabidopsis thaliana in the present invention.
图中1.微波器壳，2.数据处理电脑，3.燃烧控制器，4.微波处理舱，5.微波发生器，6.微波处理舱门，7.拟南芥放置转盘，8.电源开关，9.电压控制旋钮，10.模式调节旋钮，11.数据显示板，12.微波释放板，13.壳顶透气孔，14.微波器电源线，15.微波器电源插头，16.微波处理舱门把手，17.光电倍增管群，18.壳顶，19.光子探测器，20.数据传输线，21.电脑底座，22.电脑电源线，23.电脑电源插头。In the figure 1. Microwave shell, 2. Data processing computer, 3. Combustion controller, 4. Microwave processing cabin, 5. Microwave generator, 6. Microwave processing cabin door, 7. Arabidopsis placing turntable, 8. Power supply Switch, 9. Voltage control knob, 10. Mode adjustment knob, 11. Data display board, 12. Microwave release board, 13. Vent hole on the top of the shell, 14. Microwave power cord, 15. Microwave power plug, 16. Microwave Processing hatch handle, 17. Photomultiplier tube group, 18. Shell top, 19. Photon detector, 20. Data transmission line, 21. Computer base, 22. Computer power cord, 23. Computer power plug.
具体实施方式Detailed ways
实施例一：Embodiment one:
如图所示，本发明的利用医学影像技术研究模式植物拟南芥盐分处理机制的方法具体为：利用医学影像技术研究模式植物拟南芥盐分处理机制的方法由拟南芥植株栽培、纳米金属颗粒摄入和处理装置三部分构成。As shown in the figure, the method for using medical imaging technology to study the salt processing mechanism of the model plant Arabidopsis thaliana in the present invention is specifically: the method for using medical imaging technology to study the salt processing mechanism of the model plant Arabidopsis thaliana is cultivated by Arabidopsis plants, nano-metal The particle intake and processing unit consists of three parts.
一、拟南芥植株栽培1. Arabidopsis plant cultivation
1.播种和发芽1. Sowing and Germinating
拟南芥可在非无菌条件下，生长在土壤或人工配制的各种培养基中。作为栽植的容器，可根据各自条件置于花盆或格状分离的多穴塑料盘中。常用的混合物有泥炭藓、营养土、蛭石和珍珠岩等，例如珍珠 岩∶蛭石∶泥炭藓 =1∶1∶1的混合物，表土、堆肥或腐殖质土∶珍珠岩或蛭石=1∶1，1∶2或 2∶1的混合物。如果用于营养研究则可以蛭石类惰性物质作培养介质，施以配有营养物质的水溶液。栽培拟南芥的介质均要求有良好的排水性，因此一般混合砂子、蛭石等惰性介质，保持良好的排水，防止过湿引起真菌和昆虫幼虫滋生。播种前土壤混合物进行高压灭菌处理30min，以杀死可能存在于混合物中的任何害虫。在把土壤混合物置于花盆或其他容器中后，将整个容器置于水或营养液中，靠毛细管作用浸湿介质，然后将处理洗净来的种子，用尖头烧融后的移液管小心移至土表，均匀播下。如果播种量较大，可用浓度为0.1g/100mL琼脂或砂子事先均匀混合后播种。种子发芽期间必须保持高湿度，故容器可用塑料膜覆盖，保持一周左右方可揭去。将播有种子的容器移至低温或相应低温条件下，在2～4℃下放置2～4d，从而于吸胀条件下破除种子休眠，这对新鲜收获的拟南芥种子尤为必要。对大多数拟南芥品系来说其种子是中度休眠 的，收获已久的这类生态型的拟南芥种子可免于低温处理，而有些生态型甚至需长达7d的低温处理。干种子的低温处理往往是无效的。低温处理后，将盆移至温室或生长室，在22℃左右发芽，夜温可比日温低2℃，用2000lx的荧光灯给予光照，光周期为18h光/6h暗也可24h光照，在5d左右可见拟南芥发芽。拟南芥发芽需光，故防止种子被土覆盖。Arabidopsis can be grown in soil or artificially prepared various media under non-sterile conditions. As a planting container, it can be placed in a flower pot or a grid-shaped separated multi-hole plastic tray according to respective conditions. Commonly used mixtures include sphagnum moss, nutrient soil, vermiculite and perlite, etc., for example, a mixture of perlite: vermiculite: sphagnum moss = 1: 1: 1, topsoil, compost or humus soil: perlite or vermiculite = 1: 1 , 1:2 or 2:1 mixture. If it is used for nutritional research, vermiculite-like inert substances can be used as a culture medium, and an aqueous solution with nutrients can be applied. The medium for cultivating Arabidopsis requires good drainage. Therefore, inert media such as sand and vermiculite are generally mixed to maintain good drainage and prevent over-humidity from causing fungi and insect larvae to breed. The soil mixture was autoclaved for 30 minutes before sowing to kill any pests that might be present in the mixture. After placing the soil mixture in a flowerpot or other container, place the entire container in water or nutrient solution, soak the medium by capillary action, and then pipette the cleaned seeds with a pointed tip Carefully move the tube to the soil surface and sow evenly. If the amount of seeding is large, the concentration of 0.1g/100mL agar or sand can be mixed evenly before sowing. High humidity must be maintained during seed germination, so the container can be covered with plastic film and kept for about a week before being removed. Move the container with the seeds to low temperature or corresponding low temperature conditions, and place it at 2-4°C for 2-4 days, so as to break the seed dormancy under imbibition conditions, which is especially necessary for freshly harvested Arabidopsis seeds. The seeds of most Arabidopsis lines are moderately dormant, and Arabidopsis seeds of this ecotype that have been harvested for a long time can be exempted from low temperature treatment, and some ecotypes even require low temperature treatment for up to 7 days. Low temperature treatments of dried seeds are often ineffective. After the low temperature treatment, move the pots to the greenhouse or growth room, germinate at about 22°C, the night temperature can be 2°C lower than the day temperature, and give light with 2000lx fluorescent lamps, the photoperiod is 18h light/6h dark or 24h light, in 5 days Germination of Arabidopsis thaliana can be seen on the left and right. Arabidopsis requires light to germinate, so prevent the seeds from being covered with soil.
2. 生长发育条件的控制2. Control of growth and development conditions
拟南芥一般是冬性一年生植物，自然条件下种子在秋天发芽，幼年期度过冬天，花分生组织在春季分化，种子在夏季成熟脱落。大多数实验室栽植的拟南芥品种在发芽后4周开花，而在4～6周后采集种子。不同拟南芥生态型其发育进程快慢、开花时间早晚、何时成熟等除了取决于遗传性以外，也受外界环境条件的影响。Arabidopsis is generally a winter annual plant. Under natural conditions, seeds germinate in autumn and spend the winter as juveniles. Floral meristems differentiate in spring, and seeds mature and fall off in summer. Most laboratory-grown Arabidopsis varieties flowered 4 weeks after germination, and seeds were collected 4 to 6 weeks later. The speed of development, flowering time, and maturity of different Arabidopsis ecotypes are not only determined by heredity, but also affected by external environmental conditions.
2.1光：光对拟南芥生长的影响涉及光强和光周期两个方面。以光强度说，在生长室中一般最适光的光强度为120～150μmol/㎡ · s1μmol/㎡· s=5烛光 =0．217W/m 2 =53．8lx，这可通过荧光灯，配以白炽光来达到。在夏天温室中，60%荫影有助于光强控制和温度调节，高光强或直接太阳光照射对较老植株可以忍受，而年幼植株避免强光。拟南芥在连续照光和 长日下开花加快，短日时开花被 阻遏或延迟，这表明拟南芥开花需要长日照光周期，一般至少12h的光照。在冬季温室中可补充早晚的光照，以满足光周期需要，一般给以16h光照，8h暗期为宜。连续光照可促进生殖循环，略微提早开花，但使叶数减少及降低种生成，而较短日照有利于营养生长。2.1 Light: The influence of light on the growth of Arabidopsis involves two aspects: light intensity and photoperiod. In terms of light intensity, the most suitable light intensity in the growth chamber is generally 120-150μmol/㎡·s1μmol/㎡·s=5 candlelight=0.217W/m 2 =53.8lx, which can be achieved by fluorescent lamps, coupled with incandescent light to achieve. In the summer greenhouse, 60% shade is helpful for light intensity control and temperature regulation. High light intensity or direct sunlight can be tolerated by older plants, while young plants avoid strong light. The flowering of Arabidopsis thaliana was accelerated under continuous light and long days, and flowering was inhibited or delayed under short days, which indicated that Arabidopsis flowering required long-day photoperiods, generally at least 12 hours of light. In the winter greenhouse, the light in the morning and evening can be supplemented to meet the needs of the photoperiod. Generally, 16 hours of light and 8 hours of darkness are appropriate. Continuous light can promote the reproductive cycle and flower slightly earlier, but reduce the number of leaves and reduce seed production, while shorter days are conducive to vegetative growth.
2.2 温度：温室和生长室的温度应予以控制，最适生长温度为25℃左右，稍低的温度也是允许的。当水分供应充足时，植物甚至能在高达34℃时生长，但会减少受精。较老的植物能忍受高温，但保持25℃对整个生长周期是有利的。当种子形成时，生长室温度宜设定在25℃，而温室温度宜在23℃，夜温可比日温低2～4℃为宜。对于许多迟开花的拟南芥生态型来说，幼苗期要给以4℃左右处理一个时期如几周，以完成春化作用，从而在长日下促进开花。而对于常用的拟南芥生态型Landsbergerecta和Columbia则不需作春化处理就能开花。必须注意这里的低温春化处理是不同于播种时破除休眠的低温处理，破除休眠的低温处理又称层积处理stratification。2.2 Temperature: The temperature of the greenhouse and growth chamber should be controlled. The optimum growth temperature is about 25°C, and a slightly lower temperature is also allowed. Plants can even grow at temperatures as high as 34°C when the water supply is adequate, but with reduced fertilization. Older plants can tolerate high temperatures, but maintaining 25°C is beneficial throughout the growth cycle. When the seeds are formed, the growth room temperature should be set at 25°C, while the greenhouse temperature should be at 23°C, and the night temperature should be 2-4°C lower than the day temperature. For many late-flowering Arabidopsis ecotypes, seedlings are treated at about 4°C for a period of several weeks to complete vernalization and promote flowering under long days. However, the commonly used Arabidopsis ecotypes Landsbergerecta and Columbia can flower without vernalization. It must be noted that the low-temperature vernalization treatment here is different from the low-temperature treatment that breaks dormancy during sowing, and the low-temperature treatment that breaks dormancy is also called stratification.
2.3 水分2.3 Moisture
在种子发芽后的头几周里，理想的供水是来自毛细管由下至上的渗水，只有当土壤呈现干旱时适时灌溉。过量供水会引起土表藻类和真菌的生长。在拟南芥头两片真叶开始伸展之前必须避免干旱，当真叶长出后，灌水频率可相应减少，如每周一或两次，而至长角果充实阶段必须保证水分供应，以利于种子形成。浇水时最好待90%左右的穴盘或花盆完全干燥之后进行。不仅土壤供水状况影响到拟南芥的生长发育，而且湿度也会影响水分供给。虽然湿度的增加如50% ～60%会大大减少土表干旱的影响以及发芽着的幼苗脱水危害，但一般说来拟南芥植株，包括幼苗都能忍受低湿度，处在莲座状阶段的植株可在不同湿度下生长，当长角果进入成熟阶段时，较低湿度如<50%是有利的。During the first few weeks after germination, the ideal water supply is from bottom-up capillary seepage, watering only when the soil appears dry. Excessive water supply can cause the growth of algae and fungi on the soil surface. Drought must be avoided before the first two true leaves of Arabidopsis thaliana start to stretch. After the true leaves grow, the irrigation frequency can be reduced accordingly, such as once or twice a week, and the water supply must be guaranteed at the silique full stage to benefit the seeds. form. When watering, it is best to wait until about 90% of the plugs or flowerpots are completely dry. Not only the soil water supply status affects the growth and development of Arabidopsis, but also the humidity will affect the water supply. Although an increase in humidity such as 50% to 60% will greatly reduce the impact of drought on the soil surface and the dehydration hazards of germinating seedlings, in general Arabidopsis plants, including seedlings, can tolerate low humidity, and plants in the rosette stage Can be grown in different humidity levels, lower humidity such as <50% is beneficial when siliques enter the ripening stage.
2.4 营养2.4 Nutrition
正常情况下只要配置合适的土壤混合物，就并非必须供给营养物质，但是贫瘠的营养状况会降低植株高度，使它提早开花，并使种子着生减少。在生长发育的后期阶段补充营养物质将会增加种子着生，并产生较健壮的植 株。当植株呈现出轻微淡绿色时，表明营养供给不 足，则应立即施以营养物质，正常健壮的拟南芥植株是亮暗绿色的。Normally, with the right soil mix, it is not necessary to provide nutrients, but poor nutrient status will reduce plant height, cause it to flower earlier, and reduce seed set. Nutrient supplementation at later stages of growth and development will increase seed settling and produce stronger plants. When the plant shows a slight light green, it indicates that the nutrient supply is insufficient, and the nutrient should be applied immediately. The normal and healthy Arabidopsis plants are bright and dark green.
2.5 防止杂交2.5 Prevent hybridization
拟南芥是自交授粉的，为了保持拟南芥品系的纯化，必须防止温室或生长室中各品系之间的杂交。为此可根据各自实验室条件进行设置。例如，保持生长环境的清洁，从而防止经昆虫载体而导致的杂交机会。栽植时注意各品系种植之间的距离如20cm，从而防止来自不同品系的花互相接触。在长成植株后，可采取适当措施，防止植株倒伏，以致互相接触。Arabidopsis thaliana is self-pollinating and in order to maintain the purity of Arabidopsis lines it is necessary to prevent crossing between the lines in the greenhouse or growth chamber. This can be set according to the respective laboratory conditions. For example, keeping the growing environment clean prevents the chance of hybridization via insect vectors. When planting, pay attention to the distance between the planting of each line, such as 20cm, so as to prevent the flowers from different lines from touching each other. After growing into plants, appropriate measures can be taken to prevent the plants from falling down and touching each other.
二、纳米金属颗粒摄入2. Ingestion of Nano Metal Particles
根据研究目的，选择需要研究的盐分，采用公知的技术，制备或购买市售的该种直径为10-100纳米的纳米金属颗粒，比如研究铁盐，则加入直径为10-100纳米的纳米铁颗粒。然后，将纳米金属颗粒混入拟南芥培养液，使纳米金属颗粒随水分由根吸收进拟南芥植物体内。三、处理装置According to the purpose of the research, select the salt to be studied, and use known techniques to prepare or purchase commercially available nano-metal particles with a diameter of 10-100 nanometers. For example, to study iron salts, add nano-iron particles with a diameter of 10-100 nanometers particles. Then, the nano-metal particles are mixed into the Arabidopsis culture solution, so that the nano-metal particles are absorbed into the Arabidopsis plant from the root along with the water. 3. Processing device
处理装置由微波器壳1、数据处理电脑2、燃烧控制器3、微波处理舱4、微波发生器5、微波处理舱门6、拟南芥放置转盘7、电源开关8、电压控制旋钮9、模式调节旋钮10、数据显示板11、微波释放板12、壳顶透气孔13、微波器电源线14、微波器电源插头15、微波处理舱门把手16、光电倍增管群17、壳顶18、光子探测器19、数据传输线20、电脑底座21、电脑电源线22、电脑电源插头23组成。微波器壳1，由涂覆非磁性材料的金属板制成，长方体形，长度为50-100厘米，宽度为30-60厘米，高度为40-80厘米，内有燃烧控制器3、微波处理舱4和光谱采集器19。数据处理电脑2、光子探测器19、数据传输线20、电脑底座21、电脑电源线22和电脑电源插头23组成公知的光学多道分析仪OMA (Optical Multi-channel Analyzer)，该光学多道分析仪OMA是采用光子探测器和计算机控制的光谱分析仪器,它集信息采集,处理,存储诸功能于一体.由于OMA不再使用感光乳胶,避免和省去了暗室处理以及之后的一系列繁琐处理,测量工作，相比传统的光谱技术，光学多道分析仪OMA改善了工作条件,提高了工作效率;使用OMA分析光谱,测量准确迅速,方便,且灵敏度高,响应时间快,光谱分辨率高,测量结果可立即从显示屏上读出或由打印机,绘图仪输出。光子探测器19结构原理同公知的光子探测器(CCD)，主要组成部件为光电倍增管群17，用于信息采集,处理，并将处理后的图像信息由数据传输线20传输给数据处理电脑2。光电倍增管群17由多个光电倍增管组成，光电倍增管是依据光电子发射、二次电子发射和电子光学的原理制成的、透明真空壳体内装有特殊电极的器件，是进一步提高光电管灵敏度的光电转换器件，管内除光电阴极和阳极外，两极间还放置多个瓦形倍增电极，使用时相邻两倍增电极间均加有电压用来加速电子。光电阴极受光照后释放出光电子，在电场作用下射向第一倍增电极，引起电子的二次发射，激发出更多的电子，然后在电场作用下飞向下一个倍增电极，又激发出更多的电子。如此电子数不断倍增，阳极最后收集到的电子可增加104～108倍，整个过程时间约10-8秒。这使光电倍增管的灵敏度比普通光电管要高得多，可用来检测微弱光信号。数据处理电脑2则进一步处理并存储相关信息，供使用者选择使用。光学多道分析仪OMA的电能由电脑电源线22和电脑电源插头23从外接电源插座引入。燃烧控制器3包括微波发生器5、电源开关8、电压控制旋钮9、模式调节旋钮10、数据显示板11、微波释放板12、微波器电源线14、微波器电源插头15。微波发生器5同公知微波炉的磁控管、波导和电源变压器：电源变压器是给磁控管提供电压的部件；磁控管实际上是一个真空管金属管，由它产生和发射微波直流电能转换成微波震荡输出；波导则是将磁控管产生的微波功率传输到微波处理舱4，以加热拟南芥体内的纳米金属颗粒使之燃烧发光。电源开关8控制微波器电源线14由微波器电源插头15从外接插座上引过来的电能。电压控制旋钮9同公知微波炉的电压控制旋钮，控制燃烧控制器3的电压，需要高电压则调到高电压处，需要低电压则调到低电压处。模式调节旋钮10同公知微波炉的时间控制旋钮，可以根据拟南芥植株的大小选择不同的刻度，主要是作用时间。数据显示板11同公知的液晶显示屏，可以显示时间的变化及电压的高低。微波释放板12是直径为10-15厘米的圆形具孔区域，位于微波处理舱4与微波发生器5相连的侧壁上，使微波发生器5产生的微波均匀地释放到微波处理舱4中。微波处理舱4：也称谐振腔，它是处理拟南芥植株的地方，经波导管输入炉腔内的微波在腔壁内来回反射，每次传播都穿过和经过拟南芥植株。在设计时，通常使微波处理舱4的边长为1/2微波导波波长的倍数，这样使拟南芥植株被加热时，舱内能保持谐振，谐振范围适当变宽。在微波处理舱4的顶部开有2个壳顶透气孔13。微波处理舱门6的作用是便于取放拟南芥植株及观察处理时的情形，微波处理舱门6又是构成微波处理舱4的前壁，它是防止微波泄露的一道关卡。拟南芥放置转盘7是安装在微波处理舱4的底部，离炉底有的高度为1-1.5厘米，由一只以5-6转/分钟转速的小马达带动。所述的处理装置在使用时，首先利用微波将拟南芥植株体内的直径为10-100纳米的纳米金属颗粒加热燃烧，然后利用光学多道分析仪OMA采集、处理、分析所得数据，在电脑上显示出纳米金属颗粒在拟南芥植株体内不同部位的分布情况，进而利用医学影像技术的原理和方法直观地推测拟南芥植株盐分处理的机制。The processing device consists of a microwave shell 1, a data processing computer 2, a combustion controller 3, a microwave processing cabin 4, a microwave generator 5, a microwave processing cabin door 6, a turntable for placing Arabidopsis thaliana 7, a power switch 8, a voltage control knob 9, Mode adjustment knob 10, data display board 11, microwave release board 12, shell top air vent 13, microwave power cord 14, microwave power plug 15, microwave processing cabin door handle 16, photomultiplier tube group 17, shell top 18, Photon detector 19, data transmission line 20, computer base 21, computer power cord 22, computer power plug 23 are formed. Microwave oven shell 1, made of metal plate coated with non-magnetic material, cuboid shape, length 50-100 cm, width 30-60 cm, height 40-80 cm, combustion controller 3, microwave treatment cabin 4 and spectrum collector 19. Data processing computer 2, photon detector 19, data transmission line 20, computer base 21, computer power cord 22 and computer power plug 23 form known optical multi-channel analyzer OMA (Optical Multi-channel Analyzer), this optical multi-channel analyzer OMA is a spectral analysis instrument using photon detectors and computer control. It integrates information collection, processing, and storage functions. Since OMA no longer uses photosensitive emulsion, it avoids and saves darkroom processing and a series of cumbersome processing afterwards. Measurement work, compared with the traditional spectrum technology, the optical multi-channel analyzer OMA improves the working conditions and improves the work efficiency; using OMA to analyze the spectrum, the measurement is accurate, fast, convenient, and has high sensitivity, fast response time, and high spectral resolution. Measurement results can be read immediately from the display or output by a printer or plotter. The structural principle of the photon detector 19 is the same as that of the known photon detector (CCD). The main component is the photomultiplier tube group 17, which is used for information collection and processing, and the processed image information is transmitted to the data processing computer 2 by the data transmission line 20 . The photomultiplier tube group 17 is composed of a plurality of photomultiplier tubes. The photomultiplier tube is made according to the principle of photoelectron emission, secondary electron emission and electron optics. Sensitive photoelectric conversion device, in addition to the photocathode and anode in the tube, multiple tile-shaped dynodes are placed between the two poles. When in use, a voltage is applied between adjacent double dynodes to accelerate electrons. The photocathode releases photoelectrons after being illuminated, and shoots to the first dynode under the action of the electric field, causing the secondary emission of electrons, stimulating more electrons, and then flying to the next dynode under the action of the electric field, which excites more electrons. Many electrons. In this way, the number of electrons is continuously multiplied, and the electrons finally collected by the anode can increase by 104 to 108 times, and the whole process takes about 10-8 seconds. This makes the sensitivity of the photomultiplier tube much higher than that of ordinary phototubes, which can be used to detect weak light signals. The data processing computer 2 further processes and stores relevant information for users to choose and use. The electrical energy of the optical multi-channel analyzer OMA is introduced from an external power socket by a computer power cord 22 and a computer power plug 23 . The combustion controller 3 includes a microwave generator 5, a power switch 8, a voltage control knob 9, a mode adjustment knob 10, a data display board 11, a microwave release board 12, a microwave power cord 14, and a microwave power plug 15. The microwave generator 5 is the same as the magnetron, waveguide and power transformer of the known microwave oven: the power transformer is the part that provides voltage to the magnetron; The microwave oscillates output; the waveguide transmits the microwave power generated by the magnetron to the microwave treatment chamber 4, so as to heat the nano metal particles in the Arabidopsis to burn and emit light. The power switch 8 controls the electric energy that the microwave power supply line 14 draws from the external socket by the microwave power plug 15 . Voltage control knob 9 controls the voltage of combustion controller 3 with the voltage control knob of known microwave oven, needs high voltage then is transferred to high voltage place, needs low voltage then is transferred to low voltage place. The mode adjustment knob 10 is the same as the time control knob of the known microwave oven, and different scales can be selected according to the size of the Arabidopsis plant, mainly the action time. The data display panel 11 is the same as the known liquid crystal display, which can display the change of time and the level of voltage. The microwave release plate 12 is a circular perforated area with a diameter of 10-15 centimeters, located on the side wall where the microwave treatment cabin 4 is connected to the microwave generator 5, so that the microwaves generated by the microwave generator 5 are evenly released to the microwave treatment cabin 4 middle. Microwave processing cabin 4: also called resonant cavity, it is the place where Arabidopsis plants are processed. The microwave input into the furnace cavity through the waveguide is reflected back and forth in the cavity wall, passing through and passing through the Arabidopsis plants every time. When designing, the side length of the microwave treatment cabin 4 is usually a multiple of 1/2 the wavelength of the microwave guide wave, so that when the Arabidopsis plant is heated, the cabin can maintain resonance and the resonance range is appropriately widened. On the top of the microwave processing cabin 4, there are two air holes 13 on the top of the shell. The effect of microwave treatment cabin door 6 is to be convenient to pick and place Arabidopsis plant and observe the situation during processing, and microwave treatment cabin door 6 is the front wall that constitutes microwave treatment cabin 4 again, and it is a checkpoint that prevents microwave from leaking. The Arabidopsis thaliana places the turntable 7 and is installed on the bottom of the microwave treatment cabin 4. The height that is 1-1.5 centimeters away from the bottom of the furnace is driven by a small motor with 5-6 revs/min. When the processing device is in use, microwaves are first used to heat and burn the nano-metal particles with a diameter of 10-100 nanometers in the Arabidopsis plant, and then the optical multi-channel analyzer OMA is used to collect, process, and analyze the obtained data, and the computer The distribution of nano-metal particles in different parts of Arabidopsis plants was shown on the above, and then the mechanism of salinity treatment in Arabidopsis plants was intuitively speculated by using the principles and methods of medical imaging technology.
以上显示和描述了本发明的基本原理和主要特征和本发明的优点。本行业的技术人员应了解，本发明不受上述实施例的限制，上述实施例和说明书中描述的只是说明本发明的原理，在不脱离本发明精神和范围的前提下，本发明还会有各种变化和改进，这些变化和改进都落入要求保护的本发明范围内，本发明要求保护范围由所附的权利要求书其等效物界定。The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description are only to illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Various changes and improvements fall within the scope of the claimed invention, which is defined by the appended claims and their equivalents.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510020571.6ACN104614325B (en) | 2015-01-16 | 2015-01-16 | Utilize the method for Medical Imaging Technology research mode plant Arabidopsis thaliana salinity treatment mechanism |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510020571.6ACN104614325B (en) | 2015-01-16 | 2015-01-16 | Utilize the method for Medical Imaging Technology research mode plant Arabidopsis thaliana salinity treatment mechanism |
| Publication Number | Publication Date |
|---|---|
| CN104614325A CN104614325A (en) | 2015-05-13 |
| CN104614325Btrue CN104614325B (en) | 2018-08-31 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510020571.6AExpired - Fee RelatedCN104614325B (en) | 2015-01-16 | 2015-01-16 | Utilize the method for Medical Imaging Technology research mode plant Arabidopsis thaliana salinity treatment mechanism |
| Country | Link |
|---|---|
| CN (1) | CN104614325B (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107462561B (en)* | 2017-09-09 | 2021-11-23 | 华中农业大学 | Fluorescent dynamic automatic detection device and method for tobacco leaf spot character |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5784162A (en)* | 1993-08-18 | 1998-07-21 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for biological research, medical diagnostics and therapy |
| CN101438677A (en)* | 2007-11-20 | 2009-05-27 | 张柏洲 | Device and method for treating seed |
| CN102286524A (en)* | 2011-08-08 | 2011-12-21 | 安徽农业大学 | Plant transformation vector, and construction method and application thereof |
| CN103913445A (en)* | 2013-01-08 | 2014-07-09 | 广西壮族自治区民族医药研究院(广西壮医医院) | Determination method of antineoplastic trace elements in fruit of mangrove plant avicennia marina |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5784162A (en)* | 1993-08-18 | 1998-07-21 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for biological research, medical diagnostics and therapy |
| CN101438677A (en)* | 2007-11-20 | 2009-05-27 | 张柏洲 | Device and method for treating seed |
| CN102286524A (en)* | 2011-08-08 | 2011-12-21 | 安徽农业大学 | Plant transformation vector, and construction method and application thereof |
| CN103913445A (en)* | 2013-01-08 | 2014-07-09 | 广西壮族自治区民族医药研究院(广西壮医医院) | Determination method of antineoplastic trace elements in fruit of mangrove plant avicennia marina |
| Title |
|---|
| Effects of Exogenous Abscisic Acid on Carbohydrate Metabolism and the Expression Levels of Correlative Key Enzymes in Winter Wheat under Low;Lijie LIU等;《Bioscience, Biotechnology, and Biochemistry》;20140522;第516-525页* |
| Microwave-assisted preparation of bamboo charcoal-based iron-containing adsorbents for Cr(VI) removal;X.J. Wang等;《Chemical Engineering Journa》;20111015;第326-332页* |
| 纳米氧化铜对小麦根系生理生化行为的影响;金盛杨等;《土壤》;20110430;第605-610页* |
| Publication number | Publication date |
|---|---|
| CN104614325A (en) | 2015-05-13 |
| Publication | Publication Date | Title |
|---|---|---|
| CN102972275B (en) | Technology for culturing fava bean sprout by adoption of artificial light source | |
| CN105830905A (en) | Plant hydroponic culture frame and plant growth environment protection system | |
| CN103947469B (en) | A light source control method for indoor cultivation of red bean sprouts | |
| CN106404724A (en) | Method for testing growth of cucumbers by LED (light-emitting diode) continuous light and intermittent light with different frequencies | |
| CN207692797U (en) | A kind of rice field insect investigation and sampling device | |
| CN105724228B (en) | A kind of Full-automatic simple tunable optical ultrasonic atomization plant cuttage case | |
| CN102972124B (en) | Indoor production method of radish sprouting vegetables by adopting rare-earth plant growth lamp | |
| JP2015526070A (en) | Method for stimulating plant growth, apparatus and method for calculating cumulative light quantity | |
| CN102326471B (en) | Method and system for breeding plant seedlings under compound induction of magnetic field and electric field | |
| CN101889531A (en) | A control method for indoor seedling cultivation of upland cotton | |
| CN106973787A (en) | A kind of artificial light environment cultural method of arabidopsis | |
| CN102986335A (en) | Method for raising seedling in rape chamber by utilizing LED (light-emitting diode) light source | |
| CN104614325B (en) | Utilize the method for Medical Imaging Technology research mode plant Arabidopsis thaliana salinity treatment mechanism | |
| JP6242389B2 (en) | Oxygen generation method | |
| CN202949768U (en) | System for breading plant seedlings under compound induction of magnetic field and electric field | |
| CN102972278B (en) | Method for cultivating brassica chinensis to bloom indoors with LED light source | |
| CN103947520A (en) | Indoor culture technique of mung bean sprouting vegetable | |
| CN103947521A (en) | Indoor culture method of pea sprouting vegetables | |
| JP2007202471A (en) | Moss production method | |
| CN103947517A (en) | Indoor production method of non-heading Chinese cabbages | |
| CN103960111A (en) | Indoor production technology for water planted sonchus oleraceus | |
| CN117546743A (en) | Control method of water utilization efficiency of rice based on red light-near infrared light | |
| CN103960015B (en) | A kind of method of beta vulgaris indoor production | |
| CN212459411U (en) | Novel chlorophyll fluorescence detection device | |
| Hamidon et al. | Artificial Lighting Systems for Plant Growth and Development in Indoor Farming |
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | Granted publication date:20180831 Termination date:20200116 | |
| CF01 | Termination of patent right due to non-payment of annual fee |