A kind of automatic fluorescence sheet stainerTechnical field
The present invention relates to technical field of medical inspection equipment, specifically a kind of automatic fluorescence sheet stainer.
Background technology
In recent years, as autoimmune disease is given more sustained attention, autoimmunity diagnostic products and producer also constantly gush out,Spearhead unanimously points to the most abundant BLOT class products of profit margin, but most hospital clients are in operation, also locateIn a low-down state of automatization level, detection project is numerous and diverse, and the announcement time cycle of transmitting messages is long, detection project hand-manipulatedNew detection project is increased in the excessive negative conflict for causing client；Due to specimen amount and instrument cost, the dispensing of instrumentWorking, it is fully under way to be able to again, and these imbalances between supply and demand limit the development in market.
The autoimmunity of current main flow is diagnosed by IIF (IFA), Western blot（BLOT）And enzyme exempts from method(EIA) three bulks composition.The automaticity of blotting is of a relatively high, due to Cost of Medical Treatment limitation, indirect immunofluorescence diagnosisReagent producer is less, and automatization level is relatively low, but does not automate platform.IIF is autoimmunity detectionInternationally recognized goldstandard in diagnostic method, and BLOT methods and enzyme are exempted from method and are had by new methodology instead risk.
, it is necessary to first sample is attached on slide before Immunofluorescence test is carried out, then it is badly in need of a sheet stainer of exploitation.
The content of the invention
The small automatic fluorescence sheet stainer of a kind of simple structure of present invention offer, high degree of automation, cross pollution.
The present invention is achieved through the following technical solutions：
A kind of automatic fluorescence sheet stainer, including shell, operating table surface, arm group, liquid circuit module and circuit module, the operationTable top is provided with sample rack, glass frame, reagent rack and dilution frame, and the arm group includes X arms and Y arms, and the Y arms are slided along X arms,Z arms are slidably connected on the Y arms, the liquid circuit module and circuit module are located at the operating table surface rear, and positioned at shellInside, the Y arms, Z arms and liquid circuit module are electrically connected with circuit module respectively, include liquid pin and waste liquid pin, institute on the Z armsState out liquid pin and waste liquid pin be arranged in parallel, and be slidably connected with the Z arms, it is described go out the relatively described waste liquid pin of liquid pin it is upper and lowerMotion, the liquid circuit module with it is described go out liquid pin and waste liquid pin be connected.
The Z arms also include Z arms base plate, the first motor, crosspointer support plate and direct current generator, and the Z arms base plate is perpendicularNogata is provided with Z-direction line slideway upwards, and on the one hand the crosspointer support plate is slidably connected with the Z-direction line slideway, anotherAspect is connected by the first Timing Belt with first motor, and the waste liquid pin is fixed on the crosspointer component by fixed blockOn base plate, it is described go out liquid pin be slidably connected with the fixed block by tooth bar, the direct current generator be located at the crosspointer component bottomOn plate, the output end of the direct current generator is provided with gear, and the gear is meshed with the tooth bar, first motor and direct currentMotor is electrically connected with the circuit module respectively.
It is described go out liquid pin and waste liquid pin on be arranged with fairlead, the fairlead is fixedly connected with the waste liquid pin, describedGo out liquid pin through fairlead, and slided up and down in fairlead.
The Y arms are provided with Y-direction line slideway, X to sliding block and the second motor, and the Z arms are provided with Y-direction sliding block, the ZArm is slidably connected by Y-direction sliding block with the Y-direction line slideway, and the Y-direction sliding block is by the second Timing Belt and the described second electricityMachine is connected, and the X is slidably connected to sliding block with the X arms, and second motor is electrically connected with the circuit module.
The operating table surface is provided with least one stop bit bar, the sample rack, glass frame, reagent rack and dilution frame frame inOn the stop bit bar.
The upper surface of the glass frame is provided with some slide mounting planes, and the slide mounting plane top is provided with glassPiece, the slide mounting plane both sides are provided with a groove, and the outside of the groove is located at the slide mounting planeIt is respectively arranged at the two ends with a fluting；The operating table surface is provided with two the first stop bit bars being parallel to each other, the first stop bit barSome screens are provided with, the lower surface of the glass frame is provided with support block, and the support block is snapped in the screens.
The sample rack includes base plate, first panel and second panel, the four of the base plate, first panel and second panelIndividual angle is connected by four support columns, and the support column sequentially passes through first panel, second panel and base plate from top to down, underEnd is fixed by nut, is arranged with Upper shaft sleeve and Lower shaft sleeve on every support column, the Upper shaft sleeve be located at the first panel andBetween second panel, the Lower shaft sleeve is located between the second panel and base plate, the first panel, second panel and base plateIn the vertical direction is coaxially provided with first through hole, the second through hole and third through-hole, and the diameter of the third through-hole leads to less than firstHole and the second through hole；The operating table surface is provided with two the second stop bit bars being parallel to each other, every two ends of the second stop bit barA mounting hole is respectively provided with, the lower end of the support column is snapped in the mounting hole.
The operating table surface is provided with two group of first stop bit bar, and every group of first stop bit bar includes two first stop bit bars, oneThe first stop bit bar of group is used to place glass frame, and another group of the first stop bit bar is used to place reagent rack and dilution frame, the dilution frameFrame and deep-well plates dilution frame are diluted including microwell plate.
The operating table surface is provided with washes pin pond, the liquid circuit module include fluid filling pump and drawing liquid pump, the fluid filling pump andDrawing liquid pump respectively with wash pin pond and be connected, the fluid filling pump with it is described go out liquid pin be connected, the drawing liquid pump and the waste liquid pinIt is connected, the fluid filling pump and drawing liquid pump are electrically connected with circuit module respectively.
The shell is renovated including one, and described renovating is connected with shell rotation, described to renovate positioned at the upper of operating table surfaceSide and front, and described renovate transparent shape.
The beneficial effect brought of the present invention is：
In the present invention, the automatic fluorescence sheet stainer includes shell, operating table surface, arm group, liquid circuit module and circuit module,The operating table surface is provided with sample rack, glass frame, reagent rack and dilution frame, and the arm group includes X arms, Y arms and Z arms, wherein ZIt is provided with out liquid pin and waste liquid pin on arm, and then realizes out liquid pin and waste liquid pin in X to the movement in, Y-direction and Z-direction, completesSample Dilution, it is loaded, washes pin, cleaning slide, the operating procedure such as reagent adding, the circuit module plays synthetically control action,The liquid circuit module is mainly the discharge of control cleaning fluid and waste liquid；The double needle configuration of liquid pin and waste liquid pin is set out on Z arms,On the one hand slide can be cleaned multiple times, another aspect double needle configuration can reduce cross pollution, it is described go out liquid pin be used forSample-adding, reagent adding and output cleaning fluid, the waste liquid pin are used to for the cleaning fluid on slide to detach slide, and crosspointer respectiveUse during there is difference in height.
Brief description of the drawings
The present invention is described in further detail below in conjunction with accompanying drawing.
Fig. 1 is the structural representation of automatic fluorescence sheet stainer closed mode of the present invention.
Fig. 2 is the structural representation of automatic fluorescence sheet stainer open mode of the present invention.
Fig. 3 is the assembling figure of Y arms of the present invention and Z arms.
Fig. 4 is the structural representation of Z arms of the present invention.
Fig. 5 is the explosive view of operating table surface of the present invention and sample rack, glass frame, reagent rack and dilution frame.
Fig. 6 is the assembling figure of glass frame of the present invention and slide.
Fig. 7 is the structural representation of glass frame of the present invention.
Fig. 8 is the profile of sample rack of the present invention.
Fig. 9 is the structural representation of sample rack reverse side of the present invention.
Figure 10 is the structural representation of reagent rack of the present invention.
Figure 11 is the structural representation that deep-well plates of the present invention dilute frame.
Figure 12 is the structural representation that microwell plate of the present invention dilutes frame.
The corresponding label of component names is as follows in figure：
1st, shell；2nd, operating table surface；3rd, sample rack；4th, glass frame；5th, reagent rack；6th, frame is diluted；7th, X arms；8th, Y arms；9、ZArm；10th, liquid pin is gone out；11st, waste liquid pin；12nd, Z arms base plate；13rd, the first motor；14th, crosspointer support plate；15th, direct current generator；16、Z-direction line slideway；17th, the first Timing Belt；18th, fixed block；19th, tooth bar；20th, gear；21st, fairlead；22nd, Y-direction line slideway；23rd, X is to sliding block；24th, the second motor；25th, Y-direction sliding block；26th, the second Timing Belt；27th, slide mounting plane；28th, slide；29th, it is recessedGroove；30th, slot；31st, the first stop bit bar；32nd, screens；33rd, support block；34th, base plate；35th, first panel；36th, second panel；37th, support column；38th, nut；39th, Upper shaft sleeve；40th, Lower shaft sleeve；41st, first through hole；42nd, the second through hole；43rd, third through-hole；44、Second stop bit bar；45th, mounting hole；46th, pin pond is washed；47th, fluid filling pump；48th, drawing liquid pump；49th, renovate；50th, convex tendon；51st, microwell plateDilution frame；52nd, deep-well plates dilution frame.
Specific embodiment
Below in conjunction with the accompanying drawings and embodiment the present invention is described in further detail：
As the embodiment of automatic fluorescence sheet stainer of the present invention, as shown in Figure 1, Figure 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7, figure8th, shown in Fig. 9, Figure 10, Figure 11 and Figure 12, including shell 1, operating table surface 2, arm group, liquid circuit module and circuit module, the behaviourMake table top and be provided with sample rack 3, glass frame 4, reagent rack 5 and dilution frame 6, the arm group includes X arms 7 and Y arms 8, the Y arms 8Slided along X arms 7, Z arms 9 are slidably connected on the Y arms 8, after the liquid circuit module and circuit module are located at the operating table surface 2Side, and inside shell 1, the Y arms 8, Z arms 9 and liquid circuit module are electrically connected with circuit module respectively, are included on the Z arms 9Go out liquid pin 10 and waste liquid pin 11, it is described go out liquid pin 10 and waste liquid pin 11 it is arranged in parallel, and be slidably connected with the Z arms 9, instituteThe relatively described waste liquid pin 11 of liquid pin 10 is stated out to move up and down, the liquid circuit module with it is described go out liquid pin 10 and waste liquid pin 11 be connectedIt is logical.The double needle configuration of liquid pin 10 and waste liquid pin 11 is set out on Z arms 9, on the one hand slide 27 can be cleaned multiple times, separatelyOne side double needle configuration can reduce cross pollution, it is described go out liquid pin 10 be used to being loaded, reagent adding and output cleaning fluid, it is described uselessLiquid pin 11 is used to for the cleaning fluid on slide to detach slide, and crosspointer has difference in height during respective use；In addition, it is described go out liquid needle set have detection liquid level function.
In the present embodiment, the Z arms 9 also include Z arms base plate 12, the first motor 13, crosspointer support plate 14 and direct currentMachine 15, the in the vertical direction of Z arms base plate 12 is provided with Z-direction line slideway 16, the one side of crosspointer support plate 14 and instituteState Z-direction line slideway 16 to be slidably connected, be on the other hand connected with first motor 13 by the first Timing Belt 17, it is described uselessLiquid pin 11 is fixed on the crosspointer support plate 14 by fixed block 18, it is described go out liquid pin 10 pass through tooth bar 19 and the fixationBlock 18 is slidably connected, and on the crosspointer support plate 14, the output end of the direct current generator 15 sets the direct current generator 15Have gear 20, the gear 20 is meshed with the tooth bar 19, first motor 13 and direct current generator 15 respectively with the electricityRoad module electrical connection.The crosspointer support plate 14 drives out liquid pin 10 and waste liquid pin 11 simultaneously under the driving of the first motor 13Move up and down, when needing sample-adding, reagent adding or adding cleaning fluid, the direct current generator 15 is rotated, by gear 20 and tooth bar19 intermeshing, make it is described go out liquid pin 10 moved downward relative to the waste liquid pin 11, and then realize sample-adding, reagent adding and addCleaning fluid etc. operate, now, it is described go out liquid pin 10 lower end less than the waste liquid pin 11 lower end, both have a heightDifference, then effectively reduce cross pollution, when when slide is cleaned, it is described go out liquid pin 10 it is relative under the drive of direct current generator 15Waste liquid pin 11 upwards move, now, it is described go out liquid pin 10 lower end higher than the waste liquid pin 11 lower end, between the two with oneIndividual difference in height, effectively reduces cross pollution.
In the present embodiment, it is described go out liquid pin 10 and waste liquid pin 11 on be arranged with fairlead 21, the fairlead 21 with it is describedWaste liquid pin 11 is fixedly connected, it is described go out liquid pin 10 pass through fairlead 21, and slide up and down in the fairlead 21.The fairlead 21The depth of parallelism between liquid pin 10 and waste liquid pin 11 can well be ensured, prevent out liquid pin 10 to go out during up and down motionThe problem of existing position skew, is fixed with two fairleads 21 on the waste liquid pin 11, it is described go out liquid pin 10 can be in two guidingSlided up and down in set 21.
In the present embodiment, the Y arms 8 are provided with Y-direction line slideway 22, X to the motor 24 of sliding block 23 and second, the Z arms 9Y-direction sliding block 25 is provided with, the Z arms 9 are slidably connected by Y-direction sliding block 25 with the Y-direction line slideway 22, the Y-direction sliding block25 are connected by the second Timing Belt 26 with second motor 24, and the X is slidably connected to sliding block 23 with the X arms 7, describedSecond motor 24 is electrically connected with the circuit module.Under the drive of the second motor 24, the Z arms 9 can be moved along Y arms 8, Y arms 8In the 3rd motor（It is to show in figure）Drive under can be moved along X arms 7, and then realize Z arms 9 in any one position of horizontal planeThe place of putting can stop, and complete dilution, sample-adding, reagent adding, add the operation such as cleaning fluid.
In the present embodiment, the upper surface of the glass frame 4 is provided with five slide mounting planes 27, and the slide is installed flatThe top of face 27 is provided with slide 28, and the both sides of slide mounting plane 27 are provided with a groove 29, the outside of the groove 29A fluting 30 is respectively arranged at the two ends with positioned at the slide mounting plane 27；The operating table surface 2 is provided with two and is parallel to each otherThe first stop bit bar 31, the first stop bit bar 31 is provided with three screens 32, and the lower surface of the glass frame 4 is provided with supportBlock 33, the support block 33 is snapped in the screens 32.A convex tendon 50, institute are provided between two neighboring slide mounting plane 27Slide 28 is stated to be installed on slide mounting plane 27, and between adjacent two convex tendons 50, when user needs to take slide 28When lower, the two ends of slide 28 only need to be respectively pressed against by forefinger and thumb, and be located at 27 liang of slide mounting plane respectivelyEasily slide 28 can be removed in the fluting 30 at end, the effect of the fluting 30 is stepped down to forefinger and thumb；It is described recessedThe effect of groove 29 one side, can be as spillage accumulator tank when cleaning fluid overflows slide 28, and it is appropriate on the other hand can to addWater, as the evaporation of moisture carries out moisturizing to slide 28；Three screens 32 simultaneously on the first stop bit bar 31, then can be simultaneouslyThree glass frames 4 are placed, and then dye piece can be carried out simultaneously with multiple slides 28, improve the efficiency of dye piece, the support block 33Just snap in the screens 32, can be very good to realize in the horizontal direction spacing, prevent the movement of glass frame 4, and userOperation is more convenient.
In the present embodiment, the sample rack 3 includes base plate 34, first panel 35 and second panel 36, the base plate 34, theOne panel 35 is connected with four angles of second panel 36 by four support columns 37, and the support column 37 is worn successively from top to downFirst panel 35, second panel 36 and base plate 34 are crossed, lower end is fixed by nut 38, and Upper shaft sleeve is arranged with every support column 3739 and Lower shaft sleeve 40, the Upper shaft sleeve 39 is located between the first panel 35 and second panel 36, and the Lower shaft sleeve 40 is located atBetween the second panel 36 and base plate 34, the first panel 35, second panel 36 and the in the vertical direction of base plate 34 are coaxialIt is provided with first through hole 41, the second through hole 42 and third through-hole 43, the diameter of the third through-hole 43 is less than first through hole 41 and theTwo through holes 42；The operating table surface 2 is provided with two the second stop bit bars 44 being parallel to each other, every two ends of the second stop bit bar 44A mounting hole 45 is respectively provided with, the lower end of the support column 37 is snapped in the mounting hole 45.The frame of the sample rack 3 stops in secondOn the bar 44 of position, and there is position-limiting action in the horizontal direction, prevent the sample rack 3 during piece is contaminated from the feelings of displacement occurCondition.
In the present embodiment, the operating table surface 2 is provided with two group of first stop bit bar 31, and every group of first stop bit bar 31 includes twoThe first stop bit of root bar 31, one group of first stop bit bar 31 is used to place glass frame 4, and another group of the first stop bit bar is used to place reagent rack5 include that microwell plate dilutes frame 51 and deep-well plates dilute frame 52 with dilution frame 6, the dilution frame 6.Two group of first knot of stop bit bar 31Structure is identical, the card on the glass frame 4, reagent rack 5, microwell plate dilution frame 51 and the deep-well plates dilution stop bit bar 31 of frame 52 and firstThe physical dimension that position 32 is mutually clamped is also identical, therefore, the glass frame 4, reagent rack 5, microwell plate dilution frame 51 and deep-well platesDilution frame 52 can be arbitrarily placed in any one screens 32 on two group of first stop bit bar 31, so make operating table surface canDenaturation is further improved.
In the present embodiment, the operating table surface 2 is provided with washes pin pond 46, and the liquid circuit module includes fluid filling pump 47 and drawing liquidPump 48, the fluid filling pump 47 and drawing liquid pump 48 respectively with wash pin pond 46 and be connected, the fluid filling pump 47 with it is described go out the phase of liquid pin 10Connection, the drawing liquid pump 48 is connected with the waste liquid pin 11, and the fluid filling pump 47 and drawing liquid pump 48 are electric with circuit module respectivelyConnection.Cross pollution is further reduced for washing out liquid pin 10 and waste liquid pin 11 in the pin pond 46 of washing.
In the present embodiment, the shell 1 renovates 49 including one, it is described renovate 49 and rotated with shell 1 be connected, it is described to renovate49 are located at the tops and front of operating table surface 2, and described renovate 49 transparent shapes.User is being needed in operating table surface 2Sample rack 3, reagent rack 5, glass frame 4 and dilution frame 6 be when being operated, need to only open it is described renovate 49, carrying out dye pieceDuring operation, 49 are renovated in closure state, and then ensure the effect of dye piece.
Certainly, one or three fairleads are fixed with the waste liquid pin；Have four on the upper surface of the glass frameIndividual or six or eight slide mounting planes, card has a slide on each slide mounting plane.So, also all in this hairWithin bright protection domain, no longer repeat one by one here.