A kind of biology applying film and preparation methodTechnical field
The present invention relates to biological medicine and materials science field, more particularly, to a kind of biology applying film and preparation method.
Background technology
Skin damage is recurrent thing, if cannot fully treat, the lighter may leave spot after curing, sternlyBeing likely to result in of weight causes bacterium infection to cause the serious diseases such as septicemia, or even threat to life.
The various applying films that the different material in market is made are treated to wound.If gelfoam is stopping of earliest useBlood absorbent dressing, but its guiding cell growth performance is not so good as collagen.Other have promotion thin as polysaccharide, the class material such as hyaluronic acidThe function of intracellular growth, but increase with consumption, play the role of to suppress fibroblastic growth, polyurethane, terylene, nylon, silicon rubberGlue etc. be bio-inert material, non-degradable it is impossible to carry out physiological metabolism, outer layer dressing can only be used as.The biological fall such as second lactideSolution material, degradable, can metabolism, but if the little then insufficient strength of its molecular weight, molecular weight is insoluble in greatly water, during dissolving occur fallSolution, the product after the mechanical strength of impact material, and its degraded makes to organize acidity to improve about, aseptic scorchingDisease.
The certain humidity of the growth needs of skin, clinical trauma care commonly uses the surface of a wound after sterile gauze covers incrustation, butGauze can not be degraded absorption, also absorb moisture it is impossible to induced skin regeneration and restoration and with surface of a wound bonding, give when removing gauzePatient brings very big misery；Although existing market has the moist dressing of many, the risk producing is the richness in nutrition of liquidCause the growth of bacterium, due to the impact of environment and the sterilizing of dressing is insufficient or material does not have sterilizing function so that the surface of a wound is dirtyContaminate and bacterial infection, affect treatment and even cause serious harm, and adopt the easy generation of chemosterilant certain to human bodyHarm.
The dressing preparation process of various biomaterials is directed to the aseptic controlling problem of product, due to the eutrophy of materialProperty and cycle of production generally require many days, even if making the breeding of also unavoidable bacterium in an aseptic environment, so that causing productProduct microbiological contamination or endotoxin exceeded although there being many sterilization methods, major part is all to adopt chemical method, although reached disappearingThe purpose of poison, but there is the potentially harm to human body in chemosterilant.
Content of the invention
It is an object of the invention to provide a kind of biology applying film and preparation method, have cell repair effect, no antigen,Preferably human body skin surface of a wound suitability, and there are corresponding disinfection effect and the moisture-keeping functions required for therapeutic process,It is suitable for various wound surface.
In order to achieve the above object, the technical scheme of present invention offer is：A kind of biology applying film, include wet type film and doDry type film, described wet type film is made up of wet type biological raw liquor infiltration shitosan non-woven membrane, described wet type biological solutionIt is made up of collagen stoste, plants essential oil thimerosal, glycerine, chitosan powder, potassium sorbate；Described drying type film is by drying typeBe dehydrated and make after biological solution infiltration shitosan non-woven membrane, described drying type biological solution by wet type collagen stoste,Plants essential oil thimerosal, potassium sorbate composition, the preparation of described wet type film comprises the following steps:
1）Plants essential oil is pressed 2 with ethanol:1-1:1 ratio mixes, and is subsequently adding weight than the chitosan powder for 1%-5%Thick plants essential oil thimerosal is obtained after mixing；
2）The preparation of collagen：Take the tendon of mammal, remove grease, gone out with the sterilization of plants essential oil medicining liquid dippingBacterium, drains liquid after taking-up, make tendon possess certain plants essential oil thimerosal, then tendon being soaked in percent by volume isIn the acid solution of 1-5%, and add pepsin solution, make the pepsin in every milliliter of acid solution be 20-25 international activityUnit, stirs and evenly mixs, and being dipped to solution viscosity at room temperature is 500-1000cp (centipoise), filters and purifies to obtain high concentration collagen eggWhite solution；
3）The glycerine that volume ratio is 10～25%, volume ratio is added to be less than 1% mountain in described collagen solutionPotassium sorbate and volume ratio are 0.1～10% plants essential oil thimerosal, mix and pour side's planar disk into after biological solution is obtained；
4）By shitosan non-woven membrane by applying in disk infiltration after required size cutting；
5）Seal after loading in lucifuge gas impermeable bag after shitosan non-woven membrane after infiltration is drained, protect after irradiation sterilizationDeposit.
A kind of biology applying film, including wet type film and drying type film, described wet type film is infiltrated by wet type biological raw liquorShitosan non-woven membrane is made, and described wet type biological solution is gathered by collagen stoste, plants essential oil thimerosal, glycerine, shellIcing Sugar, potassium sorbate composition；Described drying type film is infiltrated to be dehydrated after shitosan non-woven membrane by drying type biological solution to be made, instituteState drying type biological solution to be made up of wet type collagen stoste, plants essential oil thimerosal, potassium sorbate, described drying type filmPreparation comprise the following steps：
1）Plants essential oil is pressed 2 with ethanol:1-1:1 ratio mixes, and is subsequently adding weight than the chitosan powder for 1%-5%Thick plants essential oil thimerosal is obtained after mixing；
2）The preparation of collagen：Take the tendon of mammal, remove grease, gone out with the sterilization of plants essential oil medicining liquid dippingBacterium, drains liquid after taking-up, make tendon possess certain plants essential oil thimerosal, then tendon being soaked in percent by volume isIn the acid solution of 1-5%, and add pepsin solution, make the pepsin in every milliliter of acid solution be 20-25 international activityUnit, stirs and evenly mixs, and being dipped to solution viscosity at room temperature is 500-1000cp (centipoise), filters and purifies to obtain high concentration collagen eggWhite solution；
3）In described collagen solution add volume ratio be less than 1% potassium sorbate and volume ratio be 0.1～10% plants essential oil thimerosal, is obtained collagen stoste after mixing, collagen stoste is poured into side's planar disk；
4）By shitosan non-woven membrane by applying in disk infiltration after required size cutting；
5）Shitosan non-woven membrane after infiltration is drained and seals after being dehydrated again in loading lucifuge gas impermeable bag after being lyophilized again, spokePreserve according to after sterilizing.
The invention has the beneficial effects as follows：The present invention not only can play shitosan to impaired skin using shitosan non-woven membraneThe repair of skin, and a carrier can be provided for other liquid substances；Chitosan powder is added not only to play to skinRepair, and thickening power can be played to solution, reach the certain sliminess of solution；Plant is added in manufacturing processThing essential oil disinfection agent, given full play to the disinfective action of plants essential oil so that the preparation process of product and use during permissibleEffectively prevent the growth of bacterium, it is to avoid product microbiological contamination and endotoxic severe overweight, relative chemical disinfectant, have preferablySecurity and validity.Repair the initial stage in wound and repair initial stage offer nutriment and sterilization using wet type film for wound,Drying type film is used to eliminate scar with more preferable wound of repairing in later stages of wound repair.
This product has needed for cell repair effect, no antigen, preferable human body skin surface of a wound suitability, therapeutic processThe corresponding disinfection effect wanted and moisture-keeping functions, are suitable for various wound surface.
Specific embodiment
Embodiment 1
A kind of biology applying film, including wet type film and drying type film, described wet type film is infiltrated by wet type biological solutionShitosan non-woven membrane is made, and described wet type biological solution is by collagen stoste, plants essential oil thimerosal, glycerine, sorbSour potassium composition；Described drying type film is infiltrated to be dehydrated after shitosan non-woven membrane by drying type biological solution to be made, described drying typeBiological solution is made up of collagen stoste, plants essential oil thimerosal, potassium sorbate.
The preparation of described wet type film comprises the following steps:
1）Plants essential oil is pressed 1 with ethanol:1 ratio mixes, and is subsequently adding weight and mixes than the chitosan powder for 1%-5%After obtain thick plants essential oil thimerosal；
2）The preparation of described collagen：Take the tendon of mammal, remove grease, disappeared with plants essential oil medicining liquid dippingPoison sterilizing, drains liquid, makes tendon possess certain plants essential oil thimerosal, then tendon is soaked in volume basis after taking-upRatio is in the acid solution for 1%, and adds pepsin solution, makes the pepsin in every milliliter of acid solution be 20 international activity listsPosition, stirs and evenly mixs, and being dipped to solution viscosity at room temperature is 500-1000cp (centipoise), filters and purifies to obtain high concentration collagenSolution；
3）In described collagen solution add volume ratio be 10% glycerine, volume ratio be 0.5 potassium sorbate andVolume ratio is 0.1% plants essential oil thimerosal, mixes and pours side's planar disk into after collagen stoste is obtained；
4）By shitosan non-woven membrane by applying in disk infiltration after required size cutting；
5）Seal after loading in lucifuge gas impermeable bag after shitosan non-woven membrane after infiltration is drained, protect after irradiation sterilizationDeposit.
The preparation of described drying type film comprises the following steps：
1）Plants essential oil is pressed 1 with ethanol:1 ratio mixes, and is subsequently adding weight and mixes than the chitosan powder for 1%-5%After obtain thick plants essential oil thimerosal；
2）The preparation of described collagen：Take the tendon of mammal, remove grease, disappeared with plants essential oil medicining liquid dippingPoison sterilizing, drains liquid, makes tendon possess certain plants essential oil thimerosal, then tendon is soaked in volume basis after taking-upRatio is in the acid solution for 1%, and adds pepsin solution, makes the pepsin in every milliliter of acid solution be 20 international activity listsPosition, stirs and evenly mixs, and being dipped to solution viscosity at room temperature is 500-1000cp (centipoise), filters and purifies to obtain high concentration collagenSolution；
3）The potassium sorbate of volume ratio 0.5% and volume ratio is added to be 0.1% plant essence in described collagen solutionOily thimerosal, is obtained collagen stoste after mixing, collagen stoste is poured into side's planar disk；
4）By shitosan non-woven membrane by applying in disk infiltration after required size cutting；
5）Shitosan non-woven membrane after infiltration is drained and seals after being dehydrated again in loading lucifuge gas impermeable bag after being lyophilized again, spokePreserve according to after sterilizing.
Embodiment 2
A kind of biology applying film, including wet type film and drying type film, described wet type film is infiltrated by wet type biological solutionShitosan non-woven membrane is made, and described wet type biological solution is by collagen stoste, plants essential oil thimerosal, glycerine, sorbSour potassium composition；Described drying type film is infiltrated to be dehydrated after shitosan non-woven membrane by drying type biological solution to be made, described drying typeBiological solution is made up of collagen stoste, plants essential oil thimerosal, potassium sorbate.
The preparation of described wet type film comprises the following steps:
1）Plants essential oil is pressed 2 with ethanol:1 ratio mixes, and is subsequently adding weight and mixes than the chitosan powder for 1%-5%After obtain thick plants essential oil thimerosal；
2）The preparation of described collagen：Take the tendon of mammal, remove grease, disappeared with plants essential oil medicining liquid dippingPoison sterilizing, drains liquid, makes tendon possess certain plants essential oil thimerosal, then tendon is soaked in volume basis after taking-upRatio is in the acid solution for 5%, and adds pepsin solution, makes the pepsin in every milliliter of acid solution be 25 international activity listsPosition, stirs and evenly mixs, and being dipped to solution viscosity at room temperature is 500-1000cp (centipoise), filters and purifies to obtain high concentration collagenSolution；
3）In described collagen solution add volume ratio be 25% glycerine, the potassium sorbate of volume ratio 0.1% andVolume ratio is 10% plants essential oil thimerosal, mixes and pours side's planar disk into after collagen stoste is obtained；
4）By shitosan non-woven membrane by applying in disk infiltration after required size cutting；
5）Seal after loading in lucifuge gas impermeable bag after shitosan non-woven membrane after infiltration is drained, protect after irradiation sterilizationDeposit.
The preparation of described drying type film comprises the following steps：
1）Plants essential oil is pressed 2 with ethanol:1 ratio mixes, and is subsequently adding weight and mixes than the chitosan powder for 1%-5%After obtain thick plants essential oil thimerosal；
2）The preparation of described collagen：Take the tendon of mammal, remove grease, disappeared with plants essential oil medicining liquid dippingPoison sterilizing, drains liquid, makes tendon possess certain plants essential oil thimerosal, then tendon is soaked in volume basis after taking-upRatio is in the acid solution for 5%, and adds pepsin solution, makes the pepsin in every milliliter of acid solution be 25 international activity listsPosition, stirs and evenly mixs, and being dipped to solution viscosity at room temperature is 500-1000cp (centipoise), filters and purifies to obtain high concentration collagenSolution；
3）The potassium sorbate that volume ratio is 0.1% and volume ratio is added to be 10% plant essence in described collagen solutionOily thimerosal, is obtained collagen stoste after mixing, collagen stoste is poured into side's planar disk；
4）By shitosan non-woven membrane by applying in disk infiltration after required size cutting；
5）Shitosan non-woven membrane after infiltration is drained and seals after being dehydrated again in loading lucifuge gas impermeable bag after being lyophilized again, spokePreserve according to after sterilizing.
Embodiment 3
A kind of biology applying film, including wet type film and drying type film, described wet type film is infiltrated by wet type biological solutionShitosan non-woven membrane is made, and described wet type biological solution is by collagen stoste, plants essential oil thimerosal, glycerine, sorbSour potassium composition；Described drying type film is infiltrated to be dehydrated after shitosan non-woven membrane by drying type biological solution to be made, described drying typeBiological solution is made up of collagen stoste, plants essential oil thimerosal, potassium sorbate.
The preparation of described wet type film comprises the following steps:
1）Plants essential oil is pressed 3 with ethanol:2 ratio mixes, and is subsequently adding weight and mixes than the chitosan powder for 1%-5%After obtain thick plants essential oil thimerosal；
2）The preparation of described collagen：Take the tendon of mammal, remove grease, disappeared with plants essential oil medicining liquid dippingPoison sterilizing, drains liquid, makes tendon possess certain plants essential oil thimerosal, then tendon is soaked in volume basis after taking-upRatio is in the acid solution for 3%, and adds pepsin solution, makes the pepsin in every milliliter of acid solution be 22 international activity listsPosition, stirs and evenly mixs, and being dipped to solution viscosity at room temperature is 500-1000cp (centipoise), filters and purifies to obtain high concentration collagenSolution；
3）The potassium sorbate that the glycerine that volume ratio is 15%, volume ratio are 0.9% is added in described collagen solutionIt is 0.6% plants essential oil thimerosal with volume ratio, mix and pour side's planar disk into after collagen stoste is obtained；
4）By shitosan non-woven membrane by applying in disk infiltration after required size cutting；
5）Seal after loading in lucifuge gas impermeable bag after shitosan non-woven membrane after infiltration is drained, after irradiation sterilizationPreserve.
The preparation of described drying type film comprises the following steps：
1）Plants essential oil is pressed 3 with ethanol:2 ratio mixes, and is subsequently adding weight and mixes than the chitosan powder for 1%-5%After obtain thick plants essential oil thimerosal；
2）The preparation of described collagen：Take the tendon of mammal, remove grease, disappeared with plants essential oil medicining liquid dippingPoison sterilizing, drains liquid, makes tendon possess certain plants essential oil thimerosal, then tendon is soaked in volume basis after taking-upRatio is in the acid solution for 3%, and adds pepsin solution, makes the pepsin in every milliliter of acid solution be 22 international activity listsPosition, stirs and evenly mixs, and being dipped to solution viscosity at room temperature is 500-1000cp (centipoise), filters and purifies to obtain high concentration collagenSolution；
3）The potassium sorbate of volume ratio 0.9% and volume ratio is added to be 0.6% plant essence in described collagen solutionOily thimerosal, is obtained collagen stoste after mixing, collagen stoste is poured into side's planar disk；
4）By shitosan non-woven membrane by applying in disk infiltration after required size cutting；
5）Shitosan non-woven membrane after infiltration is drained and seals after being dehydrated again in loading lucifuge gas impermeable bag after being lyophilized again, spokePreserve according to after sterilizing.
One of material in present invention collagen stoste, can stimulate cell division, be conducive to cell to creep to woundPosition repair deficiency, and fibroblastic growth can be suppressed, the no formation of scar；Due to biological with human body cell interstitial identicalComposition, non-stimulated to human body, allergy and allergic reaction will not be produced；This material has biodegradability, and collagen canIt is degraded to polypeptide, can be absorbed by the body, the long period can be maintained in wound, be conducive to wound healing, save the nursing time, subtractThe light misery of patient and financial burden.Collagen degradable by amino acid thus being absorbed by human body.
The chitosan powder of two of the material in the present invention, this material has good activating cell effect, promotes newly old generationThank, repair the effect of old and feeble mutant；Non-stimulated to human body, allergy and allergic reaction will not be produced；This material has lifeThing degradation property, can be absorbed by the body, and can maintain the long period in wound, be conducive to wound healing；In addition in this productAlso have certain thickening power concurrently.
Three plants essential oil thimerosals of the material in the present invention, are made up of plants essential oil and a small amount of ethanol, plants essential oilIt is Chinese conventional Chinese medicine, the therapeutic action of tool skin, and have functions that antisepsis and sterilization and anti-inflammatory, using plants essential oil as sterilizationAgent can avoid the harm that human body may be produced due to other disinfectants, and has the suitable smell of human body and antalgesic-antipruritic,Increase the comfort level of the process for the treatment of.The solution being formed after plants essential oil and ethanol mixing can make plants essential oil thimerosal mixIn biological solution, to prevent the microbiological contamination of biological solution.
The glycerine of four of the material in the present invention, in addition to having moisturizing, humectation function, also has high activity, anti-oxidant, rushThe special efficacies such as alcoholization.
The anti-corrosion function mainly to the various raw materials that product adopts for the potassium sorbate of the five of material in the present invention.
The shitosan non-woven membrane of six of the material in the present invention, this material has good activating cell effect, promotesMetabolism, repairs the effect of old and feeble mutant, is not only to play shell using the maximum benefit of shitosan non-woven membraneThe repair to damaged skin for the glycan, and a carrier can be provided for solution mass.
The to sum up description to various material functions, its design advantage shows as, and cleverly make use of various materials from differenceAngle is treated to impaired skin wound, and make use of the physical aspect of various materials mutually to rely on, and is sticked with facilitatingDamaged skin surface is protected and is repaired, and promotes skin to increase, so that the surface of a wound is fully recovered.
The present invention can have plurality of specifications size as needed, suitably partes corporis humani can divide the injury in treating of epidermis；Also may be usedNursing including skin surface.
The preparation of the present invention should be using aseptically carrying out, because manufacturing process takes pure method using material, canThe content of plants essential oil disinfectant can be affected, the resistance of material can be kept by the content being continuously replenished plants essential oil disinfectantBacterium, bacteriostasis.
Plants essential oil of the present invention includes Pogostemon cablin essential, tea tree ethereal oil, clove oil, eucalyptus quintessence oil, smokes clothingCareless essential oil, Peppermint essential oil.
Material of the present invention is commercially available, for ensureing product quality, preferably as far as possible using the specification that purity is high.Manufacture craft is collagen extraction process and common aseptic applying film manufacturing process.