A kind of in-vitro diagnosis test cardTechnical field
The present invention relates to a kind of in-vitro diagnosis test card.
Background technology
Sample test card has been used to analyze biological sample in magnetosensitive, spectrum or other automatization's reading machines.The test card about with game card size received by such reading machine, and the product of various reagent in test card with testing sample is tested.
The in-vitro diagnosis test card with fluid motion applied in the market, has two main features: one is that diagnosis liquid reagent adopts the form of the various independent packagings such as such as liquid bag to be built in instrument internal, sells separately；Two is that fluid passage adopts gummed paper to seal.
What the reagent bag of the various independent packaging of such as liquid reagent was built in instrument internal major downside is that fluid inclusion is long-pending big, when especially needing built-in plurality of liquid, the structure that liquid injects is complicated, and instrument internal has contaminated risk, and often to change liquid bag, Operation and Maintenance is inconvenient.
Assembling is more difficult to adopt the method that gummed paper seals fluid passage major downside is that, and the microparticle in gummed paper meeting adherent fluid, and in use, sample is not easily mixed homogeneously with reagent, therefore causes the limitation of application.
Shi Xizeng proposes a kind of to have fluid reservoir and hold concurrently the microfluid sample boat of pump chamber in patent CN102866257A.But in this example boat, solution storage chamber layer is to seal structure, is unfavorable for micro-fluid movement.Microfluidic channel and reative cell, waste liquid pool are arranged on flexible material layer by this example boat, but in an assembling process, will reach to seal the effect of microchannel, reative cell, waste liquid pool, it is necessary to extruding flexible material layer so that it is produce certain elastic deformation.So may result in microfluidic channel deformation, especially when microfluidic channel is only small, even can block microfluidic channel.And this example boat is double-decker, structure is complicated, and manufacturing process is complicated, adds the cost of manufacture.
Accordingly, it is desirable to provide a kind of, there is the in-vitro diagnosis test card improving structure.
Summary of the invention
It is an object of the present invention to provide a kind of new in-vitro diagnosis test card with microfluidic channel, can significantly reduce the size of instrument, improve portability and the operability of instrument.
Further object is that and a kind of new in-vitro diagnosis test card with microfluidic channel is provided, there is elastic sealing gasket and realize fully sealing liquid, reduce the risk of liquid contamination in instrument, and microfluidic channel will not microparticle in adhering liquid reagent, improve the usability of instrument.
It is yet a further object of the present invention to provide a kind of new in-vitro diagnosis test card with microfluidic channel, microfluidic channel is arranged on rigid material, it is ensured that the uniformity of passage such that it is able to guarantee the facile motion of fluid.
For reaching above-mentioned purpose, the present invention adopts following technical proposals:
A kind of in-vitro diagnosis test card, including the top cover set gradually from top to bottom, main body, elastic sealing gasket, printed circuit board (PCB) and bottom,
Described main body includes being formed the multiple reagent in top cover side, at least one sample cell and at least one waste liquid pool, and is positioned at its import of the bottom side microfluidic channel by break seal the mouth each reagent of connection and sample cell and outlet waste liquid pool；
Described elastic sealing gasket is for sealing the flow microchannel being positioned at bottom side.
Preferably, this test card farther includes the elastic sealing cover for sealing described reagent and the described sample pool being positioned at top cover side.
Preferably, described main body is positioned at the groove that top cover side farther includes to coordinate with elastic sealing cover.
Preferably, described elastic sealing cover and elastic sealing gasket are made by elastomeric material.
Preferably, described elastic sealing cover and described elastic sealing gasket are made by the rubber with smooth surface physical attribute.Passage will not microparticle in adhering liquid reagent, thus reducing the limitation of application.
Preferably, described printed circuit board (PCB) and bottom are respectively formed with multiple through hole, and the unpacking mouth that the position of each through hole is positioned at the reagent of bottom side and sample pool respectively with main body is coaxial.Unpacking head on instrument can open unpacking mouth by through hole；Unpacking on the aperture ratio instrument of through hole is slightly larger.
Preferably, described unpacking mouth is structure easily rupturable under external force.
Preferably, described unpacking mouth and main body are structure as a whole.Unpacking mouth is structure as a whole with main body, it is possible to the reagent on sealed body, sample cell, and it is easily rupturable under external force so that the liquid in reagent, sample cell flows in microfluidic channel.
Preferably, described top cover is formed with multiple through hole, and the position of each through hole is positioned at the reagent of top cover side respectively with main body and sample pool is coaxial.
Preferably, described top cover covers and is sealed by waste liquid pool in main body.
Preferably, described top cover farther includes the air-vent being positioned at above waste liquid pool.
Preferably, described bottom is connected with main body, makes described elastic sealing gasket seal described microfluidic channel.
Preferably, described top cover, main body, bottom are made by rigid material.
Beneficial effects of the present invention is as follows:
1) liquid reagent that the present invention participate in one or more in diagnosis so long be built in test card diagnostic, the injection making plurality of liquid reagent becomes simple, and can significantly reduce the size of instrument, reduce the risk of instrument internal liquid contamination, eliminate the operation changing liquid reagent bag, thus improve portability and the usability of instrument；
2) fluid microchannels of the present invention adopts cushion to seal, it is possible to suitable in various liquid reagents, the liquid reagent especially with microparticle will not stick in microchannel, thus expanding range of application, also makes assembling become simple.
3) microfluidic channel is arranged on rigid material, it is ensured that the uniformity of passage such that it is able to guarantee the facile motion of fluid.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 illustrates the structure chart of a kind of in-vitro diagnosis test card structure of the present invention；
Fig. 2 illustrates the main body axonometric chart being positioned at bottom side of a kind of in-vitro diagnosis test card structure of the present invention；
Fig. 3 illustrates the main body axonometric chart being positioned at top cover side of a kind of in-vitro diagnosis test card structure of the present invention；
Fig. 4 illustrates the bottom axonometric chart of a kind of in-vitro diagnosis test card of the present invention；
Fig. 5 illustrates the profile along liquid storage tank waste liquid pool direction of a kind of in-vitro diagnosis test card of the present invention；
Fig. 6 illustrates the top cover top perspective view of a kind of in-vitro diagnosis test card of the present invention；
Fig. 7 illustrates the top cover face upwarding stereogram of a kind of in-vitro diagnosis test card of the present invention；
Fig. 8 illustrates the front view of a kind of in-vitro diagnosis test card of the present invention；
Fig. 9 illustrates the side cut away view of a kind of in-vitro diagnosis test card of the present invention；
Figure 10 illustrates the test card axonometric chart of a kind of in-vitro diagnosis test card of the present invention；
Figure 11 illustrates the unpacking of a kind of in-vitro diagnosis test card of the present invention, fluid injection schematic diagram.
Detailed description of the invention
In order to be illustrated more clearly that the present invention, below in conjunction with preferred embodiments and drawings, the present invention is described further.Parts similar in accompanying drawing are indicated with identical accompanying drawing labelling.It will be appreciated by those skilled in the art that following specifically described content is illustrative and be not restrictive, should not limit the scope of the invention with this.
Fig. 1 show the structure chart of a kind of in-vitro diagnosis test card, and this test card includes top cover 1 from top to bottom successively, includes sealing lid 2, main body 3 and the fluid passage unit of elastic sealing gasket 4 formation, printed circuit board (PCB) 5, bottom 6.
Fig. 2 show main body 3 axonometric chart being positioned at bottom side of a kind of in-vitro diagnosis test card, and main body 3 is positioned at bottom side and is provided with microfluidic channel 301.Fig. 3 show main body 3 axonometric chart being positioned at top cover side.In this embodiment, main body 3 is positioned at top cover side and includes four reagent 307,308,309,310, a sample pool 313 and two waste liquid pools 311,312.Reagent 307,308,309,310, the bottom of sample pool 313 is provided with unpacking mouth 302,303,304,305,306.Microfluidic channel 301 is connected with reagent and sample pool by mouth of breaking seal with the import at sample pool place in each reagent.Unpacking mouthpiece has frangible structure under external force, by not making with the material of reagent generation chemical reaction and non-adhered particles.Preferably, unpacking mouth is structure as a whole with main body, and local reduction is easily to form opening under external force.It is connected with micro channels liquid outlet bottom waste liquid pool.Each reagent of the main body being positioned at top cover side is formed with injection port upwardly.Main body is provided with the groove 314 coordinated with reagent sample pool elastic sealing cover 2 at each injection port periphery.
Fig. 5 show the profile along liquid storage tank waste liquid pool direction of a kind of in-vitro diagnosis test card, elastic sealing cover 2 has the structure coordinated with the groove 314 formed in main body, act the effect sealing liquid storage tank mouth when covering in main body, its shape coordinates with reagent and sample pool.Such as, the part that elastic sealing cover 2 coordinates with the cannelure 314 in main body does wedgewise, and wedge-like tip is narrower than groove, and elastic sealing cover 2, than groove width, is pressed in cannelure 314 during assembling by tail end, seals liquid storage tank oral area by the compression of elastic sealing cover 2.Elastic sealing cover 2 has easy unlatching, easy fastening structure in the opening portion of corresponding sample pool, opens sample pool injection sample when test to facilitate.
Elastic sealing gasket 4 play seal microfluidic channel effect, its length and width size at least can main body covered on microfluidic channel and unpacking mouth.
Fig. 4 show the bottom axonometric chart of a kind of in-vitro diagnosis test card, in the present embodiment, bottom 6 is provided with supersonic welding wiring 601, for covering in main body or being connected compression printed circuit board (PCB) 5 and elastic sealing gasket 4 with main body, thus sealing microfluidic channel 301 (not shown in FIG. 4).Being provided with through hole 602,603,604,605,606 on bottom 6, its position is coaxial with the unpacking mouth of the reagent 307,308,309,310 in main body 3 and sample pool 313 respectively, for by the unpacking head on test instrunment or other openers.Preferably, the active force of unpacking head acts on unpacking mouth by elastic sealing gasket 4 so that unpacking mouth is opened.Unpacking on the aperture ratio instrument of through hole is slightly larger.
Fig. 6, Fig. 7 show top cover top perspective view and the face upwarding stereogram of a kind of in-vitro diagnosis test card, top cover 1 is provided with through hole 102,103,104,105,106, its position is coaxial with four reagent 307,308,309,310 in main body 3 and sample pool 313 respectively, it is preferable that can pass through the fluid injection pressure head on instrument.Fluid injection pressure head on the aperture ratio instrument of through hole 102,103,104,105,106 is slightly larger.Being additionally provided with supersonic welding wiring 107 on top cover 1, its effect is to be connected with main body 3, seals waste liquid pool 311,312, and compresses reagent sample pool elastic sealing cover 2；Being additionally provided with air-vent 101 on top cover 1, effect is by microfluidic channel (not shown in Fig. 6, Fig. 7) and atmosphere so that intrinsic pressure constant in fluid motor process, close with atmospheric pressure all the time.This example is provided with 3 places totally 12 little air-vents.
Fig. 8, Fig. 9, Figure 10 show the test card front view of a kind of in-vitro diagnosis test card, the side cut away view of test card, test card axonometric chart.In the present embodiment, the method assembling this magnetosensitive immunity analysis instrument test card diagnostic with microfluidic channel is as follows: be first put in main body 3 by elastic sealing gasket 4, cover microfluidic channel 301, again printed circuit board (PCB) 5 is placed on elastic sealing gasket 4, then bottom 6 is placed on printed circuit board (PCB) 5, with ultrasonic welding machine, bottom 6 and main body 3 are welded, it is achieved the elastic sealing gasket 3 sealing to microfluidic channel in main body 301.It is injected separately into quantitative particular liquid reagent in backward four reagent 307,308,309,310 of welding；Cover tightly elastic sealing cover 2；Finally top cover 1 is placed on above main body 3, it is preferable that with ultrasonic welding machine, top cover 1 and main body 3 are welded.
Figure 11 illustrates unpacking, fluid injection schematic diagram, in the present embodiment, during use, main body 3 is first positioned at the unpacking mouth 302,303,304,305 bottom the liquid storage tank 307,308,309,310 of bottom side and opens by the unpacking head on instrument, and then corresponding fluid injection pressure head injects liquid into microfluidic channel 301 by extruding the elastic sealing cover 2 on liquid storage tank 307,308,309,310 top that main body 3 is positioned at top cover side；Reciprocating extrusion also can realize the liquid reciprocal flowing in passage.
Fluid injection order during plurality of liquid can be arranged according to practical application.Preferably, unpacking head acts on liquid storage tank unpacking mouth across elastic sealing gasket and does not puncture elastic sealing gasket.Therefore the material forming elastic sealing gasket being had enough intensity, when making liquid storage tank unpacking mouth open, elastic sealing gasket does not break.
Obviously; the above embodiment of the present invention is only for clearly demonstrating example of the present invention; and be not the restriction to embodiments of the present invention; for those of ordinary skill in the field; can also make other changes in different forms on the basis of the above description; here cannot all of embodiment be given exhaustive, every belong to apparent change that technical scheme extended out or the variation row still in protection scope of the present invention.