技术领域technical field
本发明涉及美容药剂学领域，具体涉及一种抗皱嫩肤乳液及其制备方法。The invention relates to the field of cosmetic pharmacy, in particular to an anti-wrinkle and skin-rejuvenating emulsion and a preparation method thereof.
背景技术Background technique
皮肤老化是一种持续渐进的生理过程，直接影响皮肤的外观与功能。皱纹则是皮肤老化的一种标志，被称为“人生的年轮”。现代生活中的人们长期经历着高强度工作、暴露于电脑手机等高辐射以及紫外线照射环境，使得原本随年龄增长日渐暗淡的皮肤衰老的更加明显，形成小细纹、皱纹。Skin aging is a continuous and gradual physiological process that directly affects the appearance and function of the skin. Wrinkles are a sign of skin aging and are called "annual rings of life". People in modern life have experienced high-intensity work for a long time, exposure to high radiation such as computers and mobile phones, and ultraviolet radiation environments, which makes the aging of the skin that is getting darker with age more obvious, forming small fine lines and wrinkles.
皱纹的产生归结起来有四方面原因：自然老化、地心引力、光老化与光损伤、面部表情肌过多的收缩。其中最为关键的因素是自然老化和光老化两种形式，前者主要指随着年龄的增长，机体内存在的不可抗拒因素，如遗传、重力、内分泌以及免疫功能等引起的老化；后者主要由于紫外线的作用，加重了自然衰老的进程。皮肤日渐出现松弛、变薄、含水量下降，显得干燥，有鳞屑，失去光泽；弹性和坚实度也随之下降；皱纹形成，并逐渐增多，加深。此外，皮肤的敏感性增加，色素也增多。皮肤衰老的内在表现为皮肤细胞增殖能力下降，表皮更新速度减慢，皮肤重建能力减弱，皮肤胶原合成减慢，皮肤免疫力下降，色素分布不均匀等。There are four reasons for the generation of wrinkles: natural aging, gravity, photoaging and photodamage, and excessive contraction of facial expression muscles. The most critical factors are two forms of natural aging and photoaging. The former mainly refers to aging caused by irresistible factors in the body with age, such as genetics, gravity, endocrine and immune functions; the latter is mainly caused by ultraviolet rays. The effect of aggravating the natural aging process. The skin gradually becomes slack, thinner, and its water content decreases. It becomes dry, scaly, and loses luster; its elasticity and firmness also decrease; wrinkles form, and gradually increase and deepen. In addition, the sensitivity of the skin increases and pigmentation increases. The internal manifestations of skin aging are the decline of skin cell proliferation, the slowdown of epidermal renewal, the weakening of skin reconstruction ability, the slowdown of skin collagen synthesis, the decline of skin immunity, and the uneven distribution of pigment.
随着生活质量的提高，人们追求美的欲望日益增加，防衰老祛除皱纹、重返青春成了广大爱美女性的焦点话题。抗衰老要做到“防患于未然”，把促进皱纹发生的各种外界刺激因素限制到最低水平，才是防止和消除皱纹的有效途径。首先当然是保持健康的生活饮食习惯，保持愉快的心情，保证充足的睡眠，放松身心，减缓压力，才能保持肌肤健康。此外，巧妙地选取一款适合肌肤的乳液并坚持日常护理是靓丽肌肤必不可少的关键。With the improvement of the quality of life, people's desire to pursue beauty is increasing day by day. Anti-aging, wrinkle removal and rejuvenation have become the focus topics of beauty-loving women. For anti-aging, it is necessary to "prevent problems before they happen", and limit the various external stimuli that promote the occurrence of wrinkles to the lowest level, which is an effective way to prevent and eliminate wrinkles. First of all, of course, maintain healthy living and eating habits, maintain a happy mood, ensure adequate sleep, relax the body and mind, and relieve stress in order to maintain healthy skin. In addition, smartly choosing a lotion that suits the skin and sticking to the daily care are the keys to beautiful skin.
市场上现有的一些乳液中添加有害于皮肤的化学合成物质，暂时的光鲜亮丽却给以后留下了巨大皮肤隐患。Some existing emulsions on the market add chemical synthetic substances that are harmful to the skin, and the temporary radiance leaves a huge hidden danger to the skin in the future.
发明内容Contents of the invention
为解决上述技术问题，本发明提供一种含天然成分提取物的抗皱嫩肤乳液，添加天然提取成分，不含任何激素及有害化学物质，对皮肤无损伤、无刺激，缓解肌肤衰老效果更加明显。In order to solve the above technical problems, the present invention provides an anti-wrinkle and skin rejuvenating lotion containing natural extracts, adding natural extracts, does not contain any hormones and harmful chemicals, has no damage to the skin, no stimulation, and has a more obvious effect of alleviating skin aging .
本发明还提供了一种含天然成分提取物的抗皱嫩肤乳液的制备方法。The invention also provides a preparation method of the anti-wrinkle and skin-rejuvenating emulsion containing natural component extracts.
本发明提供的一种含天然成分提取物的抗皱嫩肤乳液，150g抗皱嫩肤乳液中含有以下物质：A kind of anti-wrinkle and skin-rejuvenating emulsion containing natural component extract provided by the present invention, 150g anti-wrinkle and skin-rejuvenation emulsion contains following substances:
所述西兰花汁通过以下方法制备：Described broccoli juice is prepared by following method:
选取新鲜的西兰花，用蒸馏水洗净，将其切成小朵，用蒸馏水浸泡0.3-0.5h后分次榨汁，每次取西兰花适量并加入3-6倍量蒸馏水进行榨取，合并分次榨取的西兰花汁，用洁净的双层纱布过滤3-5次，以去除汁液中的残渣，将过滤后的澄清滤液合并，体积浓缩至西兰花重量的3-5倍g/ml，再用双层纱布过滤3-5次，除去浓缩汁液中析出的残渣，制得西兰花汁备用。Select fresh broccoli, wash it with distilled water, cut it into small florets, soak it in distilled water for 0.3-0.5h, and then squeeze the juice in batches. The broccoli juice squeezed for the first time is filtered 3-5 times with a clean double-layer gauze to remove the residue in the juice, the filtered clarified filtrate is combined, and the volume is concentrated to 3-5 times the weight of the broccoli g/ml, and then Filter 3-5 times with double-layer gauze, remove the residue precipitated in the concentrated juice, and prepare broccoli juice for later use.
所述豌豆苗汁通过以下方法制备：The pea shoot juice is prepared by the following method:
选取新鲜的豌豆苗，用蒸馏水洗净，将其切成小段，用蒸馏水浸泡0.3-0.5h后，分次榨汁，每次取豌豆苗适量并加入2-4倍量蒸馏水进行榨取，合并分次榨取的豌豆苗汁，用洁净的双层纱布过滤3-5次，以去除汁液中的残渣，将过滤后的澄清滤液合并，体积浓缩至豌豆苗重量的2-4倍g/ml，再用双层纱布过滤3-5次，除去浓缩汁液中析出的残渣，制得豌豆苗汁，备用。Select fresh pea shoots, wash them with distilled water, cut them into small pieces, soak them in distilled water for 0.3-0.5h, squeeze the juice in stages, take an appropriate amount of pea shoots each time and add 2-4 times the amount of distilled water to extract, combine and divide The pea shoot juice squeezed for the first time is filtered 3-5 times with a clean double-layer gauze to remove the residue in the juice, the filtered clarified filtrate is combined, and the volume is concentrated to 2-4 times the weight of the pea shoot g/ml, and then Filter 3-5 times with double-layer gauze, remove the residue precipitated in the concentrated juice, and make pea sprout juice, set aside.
本发明提供的一种含天然成分提取物的抗皱嫩肤乳液的制备方法，包括以下步骤：The preparation method of a kind of anti-wrinkle skin rejuvenating emulsion containing natural component extract provided by the invention comprises the following steps:
a)称取海藻酸钠1.5-4.5g，加入蒸馏水30-70ml，水浴加热至60-80℃，边加热边搅拌，混合均匀后，静置让其充分溶胀，即呈透明凝胶，备用；a) Weigh 1.5-4.5g of sodium alginate, add 30-70ml of distilled water, heat in a water bath to 60-80°C, stir while heating, mix evenly, let it stand still to fully swell, that is, it becomes a transparent gel, and set aside;
b)称取卡波姆1.0-2.0g，加入蒸馏水60-100ml，水浴加热至60-80℃，边加热边迅速搅拌均匀，静置让其充分溶胀后，逐滴加入1.0-2.0g三乙醇胺中和，边加边搅拌边测PH值，当PH＝7时，即呈透明凝胶，备用；b) Weigh 1.0-2.0g of carbomer, add 60-100ml of distilled water, heat in a water bath to 60-80°C, stir quickly while heating, let it stand for full swelling, then add 1.0-2.0g of triethanolamine drop by drop Neutralize, measure the PH value while adding and stirring, when PH=7, it will become a transparent gel and set aside;
c)分别取葡萄籽油1.0-5.0ml、小麦胚芽油1.0-5.0ml、合成角鲨烷0.5-3.0ml，将三者搅拌混合均匀，再加入自乳化复合型乳化剂0.5-7.5g，于70-80℃中水浴加热，边加热边搅拌使其混合均匀，得到O/W型乳液的油相；c) Take 1.0-5.0ml of grape seed oil, 1.0-5.0ml of wheat germ oil, and 0.5-3.0ml of synthetic squalane, stir and mix them evenly, then add 0.5-7.5g of self-emulsifying compound emulsifier, Heat in a water bath at 70-80°C, stir while heating to mix evenly, and obtain the oil phase of the O/W emulsion;
d)称取泊洛沙姆0.5-5.0g，加入15-30ml蒸馏水，于70-80℃中水浴加热，边加热边搅拌，使其完全溶解，得到O/W型乳液中的水相；d) Weigh 0.5-5.0 g of poloxamer, add 15-30 ml of distilled water, heat in a water bath at 70-80° C., stir while heating to dissolve completely, and obtain the water phase in the O/W emulsion;
e)分别量取制备的西兰花汁和豌豆苗汁1.0-5.0ml，将其搅拌均匀，作为乳液的主要功效成分；e) Measure 1.0-5.0ml of the prepared broccoli juice and pea sprout juice respectively, stir them evenly, and use them as the main functional components of the emulsion;
f)分别称取10％乙二胺四乙酸二钠溶液0.01-0.5g、20％尼泊金甲酯醇溶液0.01-0.5mg、加入丙三醇1.0-8.0ml，搅拌使其混合均匀，作为乳液中的防腐剂和保湿剂；f) Weighing 0.01-0.5 g of 10% disodium edetate solution, 0.01-0.5 mg of 20% methylparaben alcohol solution, and adding 1.0-8.0 ml of glycerol, stirring to make it evenly mixed, as preservatives and humectants in lotions;
g)于70-80℃水浴中，等油相、水相达到相同温度时，将步骤c)制备的油相呈细流状沿烧杯壁缓慢加入到步骤d)制备的水相中，边加热边沿同一方匀速搅拌，15-30min后，乳化完成，即得到O/W型乳液，待乳液搅拌冷却至室温后，加入维生素C和烟酰胺各0.1-0.5g、步骤e)制备的混匀的西兰花汁和豌豆苗汁、步骤f)制备的防腐剂和保湿剂、步骤a)制备的海藻酸钠凝胶和步骤b)制备的卡波姆凝胶搅拌均匀即可。g) In a water bath at 70-80°C, when the oil phase and water phase reach the same temperature, slowly add the oil phase prepared in step c) into the water phase prepared in step d) in a thin stream along the beaker wall, while heating Stir at the same speed along the same side. After 15-30 minutes, the emulsification is completed, and the O/W emulsion is obtained. After the emulsion is stirred and cooled to room temperature, add 0.1-0.5g of vitamin C and niacinamide, and mix well prepared in step e). Broccoli juice and pea shoot juice, the preservative and humectant prepared in step f), the sodium alginate gel prepared in step a) and the carbomer gel prepared in step b) can be stirred evenly.
本发明中各成分如下：In the present invention, each composition is as follows:
西兰花含有丰富的维生素C、A、K，作为一种自由基清除剂，维生素C能发挥抗氧化功能，从而增强皮肤对外界环境的应激能力，增强皮肤免疫力，提高皮肤对有害环境的抵抗能力，还可解除有毒物质的毒性。维生素A能使皮肤保持弹性，并且能使皮肤具有抗损伤能力，可延缓皮肤衰老，有较强的美容作用。皮肤细胞衰老的根本原因是细胞经代谢过程的氧化损伤，西兰花提取物具有较强清除DPPH自由基的能力，且其水溶性提取液清除自由基的能力比脂溶性提取液能力更强。Broccoli is rich in vitamins C, A, and K. As a free radical scavenger, vitamin C can play an anti-oxidative function, thereby enhancing the skin's ability to stress the external environment, enhancing skin immunity, and improving the skin's resistance to harmful environments. It can also relieve the toxicity of toxic substances. Vitamin A can keep the skin elastic, and can make the skin have the ability to resist damage, can delay skin aging, and has a strong cosmetic effect. The root cause of skin cell aging is the oxidative damage of cells through the metabolic process. Broccoli extract has a strong ability to scavenge DPPH free radicals, and its water-soluble extract has a stronger ability to scavenge free radicals than fat-soluble extract.
豌豆苗能治疗晒黑的肌肤，使肌肤清爽不油腻。豌豆苗含有胡萝卜素、抗坏血酸、核黄素等营养物质。在豌豆苗的嫩叶中富含维生素C和能分解体内亚硝胺的酶，可以分解亚硝胺，具有抗癌防癌的作用。豌豆所含的止杈酸、赤霉素和植物凝素等物质，具有抗菌消炎，增强皮肤新陈代谢的功能。豌豆苗所含的维生素E和豌豆多糖皆具有抗氧化、防衰老的明显功效，含有的维他命B，可以作为滋润肌肤之用。Pea sprouts can treat tanned skin and make the skin refreshed and non-greasy. Pea sprouts contain nutrients such as carotene, ascorbic acid, and riboflavin. The young leaves of pea seedlings are rich in vitamin C and enzymes that can decompose nitrosamines in the body, which can decompose nitrosamines and have anti-cancer and anti-cancer effects. Pea contains anti-branch acid, gibberellin and phytolectins and other substances, which have the functions of antibacterial and anti-inflammatory, and enhance skin metabolism. The vitamin E and pea polysaccharides contained in pea sprouts have obvious effects of anti-oxidation and anti-aging, and the vitamin B contained in them can be used to moisturize the skin.
葡萄籽油作为优良的基础油，容易被皮肤吸收。它有着温和的收敛性质，可以帮助绷紧和护理皮肤。它富含亚油酸，是一种必需脂肪酸，对皮肤和细胞膜非常重要。葡萄籽油具有再生和再建功效，对皮肤有很强的保湿作用。作为最高效的抗氧化剂之一，具有清除自由基、提高人体免疫力的强力效果。它含丰富生物类黄酮、是强力的精华分子，用于对抗自由与维护微丝血管的健康。其所含大量前花青素OPC，能防护人体皮肤，免受自由基侵袭；此功能使皮肤增加弹性，不受恶劣环境侵害。葡萄籽提取物不仅有延缓衰老的作用，还是天然的阳光遮盖物，能阻挡紫外线侵害皮肤，还能够修复受伤胶原蛋白和弹性纤维。葡萄籽提取物具有收敛作用，收紧皮肤，防止皮肤皱纹的提早出现，长期使用能使皮肤光滑，富有弹性，具有美容养颜的作用。Grapeseed oil acts as an excellent base oil and is easily absorbed by the skin. It has mild astringent properties to help tighten and condition the skin. It is rich in linoleic acid, an essential fatty acid that is very important for the skin and cell membranes. Grapeseed Oil has regenerative and rebuilding properties and is a strong moisturizer for the skin. As one of the most efficient antioxidants, it has a powerful effect of scavenging free radicals and improving human immunity. It is rich in bioflavonoids, which are powerful essence molecules used to fight freedom and maintain the health of capillaries. It contains a large amount of proanthocyanidin OPC, which can protect the human skin from free radicals; this function increases the elasticity of the skin and protects it from harsh environments. Grape seed extract not only has the effect of anti-aging, but also a natural sun shield, which can block ultraviolet rays from damaging the skin, and can also repair damaged collagen and elastic fibers. Grape seed extract has astringent effect, tightens the skin, and prevents early appearance of skin wrinkles. Long-term use can make the skin smooth and elastic, and has the effect of beautifying and beautifying the skin.
小麦胚芽油集中了小麦的营养精华，富含维生素E、亚油酸、亚麻酸及多种生理活性组分，具有很高的营养价值。特别是维生素E含量为植物油之冠，已被公认为一种颇具营养保健作用的功能性油脂。其生理活性效能是合成维生素E的30倍，是天然的抗氧化剂，且易被人体吸收。在体内防止氧化脂质的生成，保护细胞膜，抑制自由基，促进人体新陈代谢，延缓机体衰老，能加强皮肤的结缔组织，增进血液循环及保持皮肤弹性，最适合于衰老和成熟的皮肤。其抗自由基的特性，可延缓皮肤老化；滋润性强，可淡化细纹、妊娠纹、疤痕，增加肌肤湿润力，对干燥、缺水、老化、皱纹肌肤极有帮助。可减少脸上长青春痘所留下的痕迹。适合各种肤质，对黑斑、干癣、湿疹有一定效果，亦具有防晒功能，可用于皮肤防晒。Wheat germ oil concentrates the nutritional essence of wheat, rich in vitamin E, linoleic acid, linolenic acid and various physiologically active components, and has high nutritional value. In particular, the content of vitamin E is the highest among vegetable oils, and it has been recognized as a functional oil with nutritional and health effects. Its physiological activity is 30 times that of synthetic vitamin E. It is a natural antioxidant and is easily absorbed by the body. Prevent the formation of oxidized lipids in the body, protect cell membranes, inhibit free radicals, promote human metabolism, delay the aging of the body, strengthen the connective tissue of the skin, improve blood circulation and maintain skin elasticity, most suitable for aging and mature skin. Its anti-free radical properties can delay skin aging; it has strong moisturizing properties, can dilute fine lines, stretch marks, and scars, increase skin moisture, and is extremely helpful for dry, dehydrated, aging, and wrinkled skin. It can reduce the marks left by acne on the face. Suitable for all skin types, it has certain effects on dark spots, psoriasis, and eczema. It also has sun protection function and can be used for skin sun protection.
角鲨烷是少有的化学稳定性高，使用感极佳的动物油脂，对皮肤有较好的亲和性，不会引起过敏和刺激，并能加速配方中其他活性成分向皮肤中渗透；具有较低的极性和中等的铺展性，且纯净、无色、无异味；还可抑制霉菌的生长。作为最接近人体皮脂的一种脂类，能够与人类自身的皮脂膜融为一体，在皮肤表面形成一层天然的屏障。它能抑制皮肤脂质的过氧化，能有效渗透入肌肤，并促进皮肤基底细胞的增殖，对延缓皮肤老化，改善并消除黄褐斑均有明显的生理效果。具有提高体内超氧化物歧化酶活性、增强机体免疫能力、抗衰老、抗疲劳、抗肿瘤等多种生理功能，是一种无毒性的具有防病治病作用的海洋生物活性物质。角鲨烷还可使皮肤毛孔张开，促进血液微循环，增进细胞的新陈代谢，帮助修复破损细胞。赋予产品极好的光亮度及梳理感觉，滋润而不油腻。Squalane is a rare animal oil with high chemical stability and excellent use feeling. It has good affinity to the skin, does not cause allergies and irritation, and can accelerate the penetration of other active ingredients in the formula into the skin; It has low polarity and medium spreadability, and is pure, colorless and odorless; it can also inhibit the growth of mold. As a lipid closest to human sebum, it can integrate with human sebum membrane to form a natural barrier on the skin surface. It can inhibit the peroxidation of skin lipids, effectively penetrate into the skin, and promote the proliferation of skin basal cells. It has obvious physiological effects on delaying skin aging, improving and eliminating chloasma. It has a variety of physiological functions such as improving the activity of superoxide dismutase in the body, enhancing the body's immunity, anti-aging, anti-fatigue, and anti-tumor. It is a non-toxic marine biological active substance with the effect of preventing and curing diseases. Squalane can also open skin pores, promote blood microcirculation, increase cell metabolism, and help repair damaged cells. Gives the product excellent brightness and combing feeling, moisturizing but not greasy.
烟酰胺也叫维生素B3，是一种水溶性稳定的小分子量物质，可以迅速穿过角质层。它可以改善皮肤的屏障作用，降低经表皮失水率；抑制黑素小体从色素细胞转移到角质形成细胞，抑制黑素输送途径，减少面部红斑、色素沉积；阻止蛋白质非酶糖化，改善面部发黄的状况；促进真皮胶原蛋白和调节真皮中黏多糖的含量，减少皱纹；通过减少油脂的分泌，减小毛孔，使皮肤纹理细腻光滑。它的祛斑美白作用表现为：加速新陈代谢，促进含黑色素的角质细胞脱落；作用于已经产生的黑色素，减少其向表层细胞转移；促进表皮层蛋白质的合成，改善肌肤质地。Niacinamide, also called vitamin B3 , is a water-soluble and stable substance with small molecular weight, which can quickly pass through the stratum corneum. It can improve the skin's barrier function, reduce the rate of water loss through the epidermis; inhibit the transfer of melanosomes from pigment cells to keratinocytes, inhibit the transport of melanin, reduce facial erythema and pigmentation; prevent non-enzymatic glycation of proteins, improve facial Yellowing condition; promote dermal collagen and regulate the content of mucopolysaccharides in the dermis, reduce wrinkles; reduce oil secretion, reduce pores, and make the skin texture fine and smooth. Its freckle-removing and whitening effect is manifested as: accelerating metabolism, promoting the exfoliation of melanin-containing keratinocytes; acting on already-produced melanin, reducing its transfer to surface cells; promoting the synthesis of epidermal protein and improving skin texture.
维生素C能抑止色素母细胞沉积，不仅可以有效预防黑斑和雀斑，还能将多余的色素排出体外，彻底改善暗哑的肤色，令肌肤重新变得白皙明亮。维生素C具有控油功效，及时给肌肤补充维生素C，可以改善肌肤油腻、黯黄，两颊干燥并泛红血丝，肤色不均匀等。还是天然的抗氧化剂，能有效控制细胞内的氧化反应，帮助人体快速排毒，让肌肤永葆青春活力。Vitamin C can inhibit the deposition of pigment mother cells, not only can effectively prevent dark spots and freckles, but also excrete excess pigment from the body, completely improve the dull complexion, and make the skin fair and bright again. Vitamin C has the effect of controlling oil, timely replenishing vitamin C to the skin can improve oily, dark yellow skin, dry cheeks with redness and bloodshot eyes, uneven skin tone, etc. It is also a natural antioxidant, which can effectively control the oxidation reaction in the cells, help the body to detoxify quickly, and keep the skin youthful and energetic.
海藻酸钠作为增稠剂和稳定剂，提高乳液的稳定性，保持乳液良好稳定的形态，减少液体渗出或收缩。它能够改善乳液内部的均一性和持水作用，起到对肌肤保湿作用。Sodium alginate acts as a thickener and stabilizer to improve the stability of the emulsion, maintain a good and stable shape of the emulsion, and reduce liquid leakage or shrinkage. It can improve the uniformity and water retention inside the emulsion, and play a role in moisturizing the skin.
卡波姆具有高效的增稠效果，能产生清澈透明的水或乙醇-水凝胶体系，适用于各种化妆品。中和后的卡波姆在很低的用量下(常规用量0.25-0.5％)就能产生高效的增稠作用，从而制备出很宽粘度范围和不同流变性的制剂。Carbomer has a high-efficiency thickening effect and can produce clear and transparent water or ethanol-hydrogel systems, which are suitable for various cosmetics. The neutralized carbomer can produce high-efficiency thickening effect at a very low dosage (conventional dosage 0.25-0.5%), thereby preparing preparations with a wide viscosity range and different rheological properties.
泊洛沙姆是一类新型的高分子非离子表面活性剂，其易溶于水，因而在乳液的水相中作为乳化剂；自乳化复合型乳化剂易溶于脂质而难溶于水，因而在乳液的油相中作为乳化剂；将两种乳化剂分别在油、水两相中按一定比例复配，形成乳化效果更佳的乳化剂。Poloxamer is a new type of polymer non-ionic surfactant, which is easily soluble in water, so it is used as an emulsifier in the water phase of the emulsion; self-emulsifying compound emulsifier is easily soluble in lipids but insoluble in water , so it is used as an emulsifier in the oil phase of the emulsion; the two emulsifiers are compounded in a certain proportion in the oil and water phases to form an emulsifier with better emulsification effect.
丙三醇又名“甘油”，具有吸水性，搽在皮肤上能形成一层薄膜，有隔绝空气和防止水分蒸发的作用，还能吸收空气中的水分。它能够使皮肤保持柔软，富有弹性，不受尘埃、气候等损害而干燥，起到防止皮肤冻伤的的作用。在各种化妆品中经常用甘油做保湿剂和软化剂。Glycerol, also known as "glycerol", is water-absorbent, and can form a thin film on the skin, which can isolate air and prevent moisture from evaporating, and can also absorb moisture in the air. It can keep the skin soft and elastic, free from dust, weather damage and dryness, and play a role in preventing skin frostbite. Glycerin is often used as a moisturizer and softener in various cosmetics.
低浓度的尼泊金甲酯就具有高效的杀菌防腐作用，防治乳液腐败变质。低浓度的乙二胺四乙酸二钠是很好的金属螯合剂，可结合乳液中游离的金属离子，提高乳液品质。A low concentration of methylparaben has a highly effective bactericidal and antiseptic effect, and prevents the emulsion from spoiling. Low concentration of disodium edetate is a very good metal chelating agent, which can bind free metal ions in the emulsion and improve the quality of the emulsion.
其抗皱抗衰老作用表现为：西兰花提取物和豌豆苗提取物中富含的多种维生素，维生素C能发挥抗氧化功能，从而增强皮肤对外界环境的应激能力，增强皮肤免疫力；维生素A能使皮肤保持弹性，并且能使皮肤具有抗损伤能力，可延缓皮肤衰老，有较强的美容作用；葡萄籽油和小麦胚芽有对肌肤具有良好的亲和性，易于吸收，使皮肤滋润而由光泽。葡萄籽提取物中的大量前花青素OPC，能够防护人体皮肤，免受自由基侵袭,此功能使皮肤增加弹性。葡萄籽提取物具有收敛作用，收紧皮肤，防止皮肤皱纹的提早出现。它还是天然的阳光遮盖物，能阻挡紫外线侵害皮肤，还能够修复受伤胶原蛋白和弹性纤维。小麦胚芽油中的天然活性成分滋润性强，可淡化细纹、妊娠纹、疤痕，增加肌肤湿润力，对干燥、缺水、老化、皱纹肌肤极有帮助。Its anti-wrinkle and anti-aging effects are as follows: the multivitamins rich in broccoli extract and pea sprouts extract, vitamin C can play an anti-oxidation function, thereby enhancing the skin's ability to respond to external stress and enhance skin immunity; A can keep the skin elastic, and can make the skin have the ability to resist damage, can delay skin aging, and has a strong cosmetic effect; grape seed oil and wheat germ have good affinity to the skin, easy to absorb, and moisturize the skin And by gloss. A large amount of proanthocyanidin OPC in grape seed extract can protect human skin from free radicals, and this function can increase skin elasticity. Grape seed extract has astringent effect, tightens the skin and prevents the early appearance of skin wrinkles. It is also a natural sunscreen that blocks UV rays from damaging the skin and repairs damaged collagen and elastic fibers. The natural active ingredients in wheat germ oil have strong moisturizing properties, can dilute fine lines, stretch marks, and scars, increase skin moisturizing power, and are extremely helpful for dry, dehydrated, aging, and wrinkled skin.
其祛斑美白作用表现为：烟酰胺可以迅速穿过角质层，抑制黑素小体从色素细胞转移到角质形成细胞，抑制黑素输送途径，减少面部红斑、色素沉积；阻止蛋白质非酶糖化，改善面部发黄的状况；促进真皮胶原蛋白和调节真皮中黏多糖的含量，减少皱纹；通过减少油脂的分泌，减小毛孔使皮肤纹理细腻光滑。角鲨烷能有效渗透入肌肤，并促进皮肤基底细胞的增殖，对延缓皮肤老化，改善并消除黄褐斑均有明显的生理效果。维生素C能抑止色素母细胞沉积，不仅可以有效预防黑斑和雀斑，还能将多余的色素排出体外，彻底改善暗哑的肤色，令肌肤重新变得白皙明亮。Its anti-freckle and whitening effect is as follows: Niacinamide can quickly pass through the stratum corneum, inhibit the transfer of melanosomes from pigment cells to keratinocytes, inhibit the transport of melanin, reduce facial erythema and pigmentation; prevent non-enzymatic glycation of proteins, improve Facial yellowing condition; promote dermal collagen and regulate the content of mucopolysaccharides in the dermis, reduce wrinkles; reduce oil secretion, reduce pores, and make the skin texture fine and smooth. Squalane can effectively penetrate into the skin and promote the proliferation of skin basal cells. It has obvious physiological effects on delaying skin aging, improving and eliminating chloasma. Vitamin C can inhibit the deposition of pigment mother cells, not only can effectively prevent dark spots and freckles, but also excrete excess pigment from the body, completely improve the dull complexion, and make the skin fair and bright again.
其滋润嫩肤效果表现为：豌豆苗可祛除皮肤分泌的过多油脂，改善皮肤的水油平衡，是皮肤清爽而不油腻。维生素C也具有控油功效，及时给肌肤补充维生素C，可以改善肌肤油腻、黯黄，两颊干燥并泛红血丝，肤色不均匀等。烟酰胺可发挥皮肤屏障作用，保持表皮水分充盈。角鲨烷作为最接近人体皮脂的一种脂类，能有效渗透入肌肤，促进血液微循环，增进细胞的新陈代谢，帮助修复破损细胞。海藻酸钠具有亲水性，可使肌肤持久锁水保湿。Its moisturizing and rejuvenating effect is as follows: pea sprouts can remove excess oil secreted by the skin, improve the water-oil balance of the skin, and make the skin refreshing and not greasy. Vitamin C also has the effect of oil control. Timely supplementing the skin with vitamin C can improve oily, dark yellow skin, dry cheeks with redness and bloodshot eyes, and uneven skin tone. Niacinamide works as a skin barrier to keep the epidermis hydrated. As a lipid closest to human sebum, squalane can effectively penetrate into the skin, promote blood microcirculation, enhance cell metabolism, and help repair damaged cells. Sodium alginate is hydrophilic, which can keep the skin moisturized for a long time.
本发明制备的乳液气味清新自然，质地均匀细腻，涂于皮肤清爽舒适轻盈易吸收。发挥紧致肌肤，修复细纹的功效，祛除色素斑的同时美白滋润肌肤，锁水保湿，令皮肤水嫩光亮，是一种极佳的天然抗皱嫩肤乳液。跟现有技术相比，本发明的乳液添加了多种天然蔬菜提取物和多种植物精华油，具有紧致细纹、促进肌肤新生、有效抗皱的功效。乳液中的其他活性物质，对祛除色素沉着、改善肌肤暗淡有良好的作用；滋润肌肤，使肌肤幼嫩细滑。而且所添加成分为直接榨取的天然新鲜蔬菜汁，气味清新，无需再添加任何香精。The emulsion prepared by the invention has fresh and natural smell, uniform and fine texture, and is refreshing, comfortable, light and easy to absorb when applied to the skin. It has the effect of firming skin and repairing fine lines, whitening and moisturizing skin while removing pigment spots, locking in moisture and moisturizing, making skin supple and bright. It is an excellent natural anti-wrinkle and skin rejuvenating lotion. Compared with the prior art, the emulsion of the present invention adds various natural vegetable extracts and various plant essential oils, and has the effects of tightening fine lines, promoting skin regeneration, and effectively anti-wrinkle. Other active substances in the lotion have a good effect on removing pigmentation and improving dull skin; it moisturizes the skin and makes the skin tender and smooth. Moreover, the added ingredient is directly squeezed natural fresh vegetable juice, which has a fresh smell and does not need to add any flavor.
具体实施方式detailed description
下面结合具体实施方案对本项发明做进一步描述。The present invention will be further described below in conjunction with specific embodiments.
本发明所用的葡萄籽油和小麦胚芽油均为市售。The used grape seed oil and wheat germ oil of the present invention are all commercially available.
实施例1Example 1
1.1西兰花汁的制备1.1 Preparation of broccoli juice
选取新鲜的西兰花，用蒸馏水洗净，将其切成小朵，用蒸馏水浸泡0.4h后分次榨汁，每次取西兰花适量并加入4倍量蒸馏水进行榨取，合并分次榨取的西兰花汁，用洁净的双层纱布过滤3次，以去除汁液中的残渣，将过滤后的澄清滤液合并，体积浓缩至西兰花重量的3倍g/ml，再用双层纱布过滤3次除去浓缩汁液中析出的残渣即得制备所需西兰花汁。Select fresh broccoli, wash it with distilled water, cut it into small florets, soak it in distilled water for 0.4h, and squeeze the juice in batches. For orchid juice, filter 3 times with clean double-layer gauze to remove the residue in the juice, combine the filtered clear filtrates, concentrate the volume to 3 times the weight of broccoli g/ml, and then filter 3 times with double-layer gauze to remove The residue precipitated from the concentrated juice can be used to prepare the desired broccoli juice.
1.2豌豆苗汁的制备1.2 Preparation of pea shoot juice
选取新鲜的嫩叶豌豆苗，用蒸馏水洗净，将其切成小段，用蒸馏水浸泡0.5h后分次榨汁，每次取豌豆苗适量并加入4倍量蒸馏水进行榨取，合并分次榨取的豌豆苗汁，用洁净的双层纱布过滤3次，以去除汁液中的残渣，将过滤后的澄清滤液合并，体积浓缩至豌豆苗重量的3倍g/ml，再用双层纱布过滤3次除去浓缩汁液中析出的残渣即得制备所需豌豆苗汁。Select fresh pea shoots with young leaves, wash them with distilled water, cut them into small pieces, soak them in distilled water for 0.5h, and squeeze the juice in batches. Pea sprouts juice, filter 3 times with clean double-layer gauze to remove the residue in the juice, combine the filtered clear filtrates, concentrate the volume to 3 times the weight of pea sprouts g/ml, and then filter 3 times with double-layer gauze The residue precipitated in the concentrated juice is removed to prepare the desired pea shoot juice.
1.3海藻酸钠凝胶的制备1.3 Preparation of sodium alginate gel
称取海藻酸钠1.5g，加入蒸馏水50ml，首先将其用水浴加热至80℃，边加热边搅拌，使得海藻酸钠完全溶解，混合均匀后，静置让其充分溶胀，得到均一稳定透明的凝胶。Weigh 1.5g of sodium alginate, add 50ml of distilled water, first heat it in a water bath to 80°C, and stir while heating to completely dissolve the sodium alginate. gel.
1.4卡波姆凝胶的制备1.4 Preparation of Carbomer Gel
称取卡波姆9401.0g，加入蒸馏水100ml，水浴加热至80℃，边加热边迅速搅拌均匀，静置让其充分溶胀后，用1.0g三乙醇胺中和，缓慢一滴滴加入，边加边搅拌边测PH值，当PH＝7时，即得粘度和稠度适宜的透明凝胶。Weigh carbomer 9401.0g, add 100ml of distilled water, heat in water bath to 80°C, stir rapidly while heating, let it swell fully, neutralize with 1.0g triethanolamine, slowly add drop by drop, stir while adding While measuring the PH value, when PH=7, a transparent gel with suitable viscosity and consistency can be obtained.
1.5乳液的制备1.5 Preparation of emulsion
a)分别取葡萄籽油1.0ml、小麦胚芽油1.0ml、合成角鲨烷0.5ml，在小烧杯中将三者搅拌均匀，再加入自乳化复合型乳化剂AC-4021.5g，于80℃中水浴加热，边加热边搅拌使其完全溶解，得到O/W型乳液中的油相；a) Take 1.0ml of grape seed oil, 1.0ml of wheat germ oil, and 0.5ml of synthetic squalane, and stir them evenly in a small beaker, then add 1.5g of self-emulsifying compound emulsifier AC-4021. Heating in a water bath, stirring while heating to dissolve completely, and obtaining the oil phase in the O/W emulsion;
b)称取泊洛沙姆0.5g，加入15ml蒸馏水，于80℃中水浴加热，边加热边搅拌，使其完全溶解，得到O/W型乳液中的水相；b) Weigh 0.5 g of poloxamer, add 15 ml of distilled water, heat in a water bath at 80° C., stir while heating to dissolve completely, and obtain the water phase in the O/W emulsion;
c)油相和水相于80℃水浴中同时加热15min后，将油相以细流状缓慢沿烧杯壁加入到水相并不断沿同一方向搅拌，速度不宜过快。20min后，乳化完成即得到O/W型乳液；c) After heating the oil phase and the water phase in a water bath at 80°C for 15 minutes at the same time, slowly add the oil phase to the water phase along the beaker wall in a thin stream and keep stirring in the same direction, but the speed should not be too fast. After 20 minutes, the emulsification is completed and the O/W emulsion is obtained;
d)西兰花汁和豌豆苗汁各1.0ml,混匀，上述乳液静置冷却至室温，加入维生素C和烟酰胺各0.2g,再加入混匀的西兰花汁和豌豆苗汁；d) 1.0ml each of broccoli juice and pea sprout juice, mix well, let the above emulsion stand and cool to room temperature, add 0.2g each of vitamin C and nicotinamide, then add the mixed broccoli juice and pea sprout juice;
e)称取10％乙二胺四乙酸二钠0.1g、20％尼泊金甲酯醇溶液0.01mg，量取丙三醇3.0ml，混合均匀，加入上述乳液中，不断搅拌使混合均匀；e) Weigh 0.1 g of 10% disodium ethylenediamine tetraacetate, 0.01 mg of 20% methylparaben alcohol solution, measure 3.0 ml of glycerol, mix them uniformly, add them to the above-mentioned emulsion, and stir continuously to mix uniformly;
f)在制得的乳液中加入上述已制备好的卡波姆凝胶、海藻酸钠凝胶，不断搅拌，制得粘度和稠度适宜的乳液。f) Add the above-mentioned prepared carbomer gel and sodium alginate gel into the prepared emulsion, and keep stirring to obtain an emulsion with suitable viscosity and consistency.
实施例2Example 2
2.1西兰花汁的制备2.1 Preparation of broccoli juice
选取新鲜的西兰花，用蒸馏水洗净，将其切成小朵，用蒸馏水浸泡0.4h后分次榨汁，每次取西兰花适量并加入，6倍量蒸馏水进行榨取，合并分次榨取的西兰花汁，用洁净的双层纱布过滤5次，以去除汁液中的残渣，将过滤后的澄清滤液合并，体积浓缩至西兰花重量的5倍g/ml，再用双层纱布过滤5次除去浓缩汁液中析出的残渣即得制备所需西兰花汁。Select fresh broccoli, wash it with distilled water, cut it into small florets, soak it in distilled water for 0.4h, and squeeze the juice in batches, take an appropriate amount of broccoli each time and add it, squeeze it with 6 times the amount of distilled water, and combine the squeezed juice Broccoli juice, filter 5 times with clean double-layer gauze to remove the residue in the juice, combine the filtered filtrates, concentrate the volume to 5 times the weight of broccoli g/ml, and then filter 5 times with double-layer gauze The residue precipitated from the concentrated juice is removed to obtain the required broccoli juice.
2.2豌豆苗汁的制备2.2 Preparation of pea shoot juice
选取新鲜的嫩叶豌豆苗，用蒸馏水洗净，将其切成小段，用蒸馏水浸泡0.5h后分次榨汁，每次取豌豆苗适量并加入6倍量蒸馏水进行榨取，合并分次榨取的豌豆苗汁，用洁净的双层纱布过滤5次，以去除汁液中的残渣，将过滤后的澄清滤液合并，体积浓缩至豌豆苗重量的5倍g/ml，再用双层纱布过滤5次除去浓缩汁液中析出的残渣即得制备所需豌豆苗汁。Select fresh pea shoots with young leaves, wash them with distilled water, cut them into small pieces, soak them in distilled water for 0.5h, and squeeze them in batches. Pea shoot juice, filter 5 times with clean double-layer gauze to remove the residue in the juice, combine the filtered clear filtrates, concentrate the volume to 5 times the weight of the pea shoot g/ml, and then filter 5 times with double-layer gauze The residue precipitated in the concentrated juice is removed to prepare the desired pea shoot juice.
2.3海藻酸钠凝胶的制备2.3 Preparation of sodium alginate gel
称取海藻酸钠4.5g，加入蒸馏水60ml，首先将其用水浴加热至75℃，边加热边搅拌，使得海藻酸钠完全溶解，混合均匀后，静置让其充分溶胀，烧杯底部无成块海藻酸钠，得到均一稳定透明的凝胶。Weigh 4.5g of sodium alginate, add 60ml of distilled water, first heat it in a water bath to 75°C, and stir while heating to completely dissolve the sodium alginate. Sodium alginate to obtain a uniform, stable and transparent gel.
2.4卡波姆凝胶的制备2.4 Preparation of Carbomer Gel
称取卡波姆9402.0g，加入蒸馏水100ml，水浴加热至75℃，边加热边迅速搅拌均匀，静置让其充分溶胀后，用2.0g三乙醇胺中和，缓慢一滴滴加入，边加边搅拌边测PH值。当PH＝7时，即得粘度和稠度适宜的透明凝胶。Weigh 9402.0g of carbomer, add 100ml of distilled water, heat in a water bath to 75°C, stir rapidly while heating, let it swell fully, neutralize with 2.0g of triethanolamine, slowly add drop by drop, stir while adding Measure the pH value. When PH=7, a transparent gel with suitable viscosity and consistency can be obtained.
2.5乳液的制备2.5 Preparation of emulsion
a)分别取葡萄籽油5.0ml、小麦胚芽油5.0ml、合成角鲨烷3.0ml，在小烧杯中将三者搅拌均匀，再加入自乳化复合型乳化剂(AC-402)3g，于75℃中水浴加热，边加热边搅拌使其完全溶解，得到O/W型乳液中的油相；a) Take 5.0ml of grape seed oil, 5.0ml of wheat germ oil, and 3.0ml of synthetic squalane respectively, stir the three evenly in a small beaker, then add 3g of self-emulsifying compound emulsifier (AC-402), and dissolve at 75 Heat in a water bath at ℃, stir while heating to dissolve completely, and obtain the oil phase in the O/W emulsion;
b)称取泊洛沙姆3.0g，加入30ml蒸馏水，于70-80℃中水浴加热，边加热边搅拌，使其完全溶解，得到O/W型乳液中的水相；b) Weigh 3.0 g of poloxamer, add 30 ml of distilled water, heat in a water bath at 70-80° C., stir while heating to completely dissolve it, and obtain the water phase in the O/W emulsion;
c)油相和水相于75℃水浴中同时加热20min后，将油相以细流状缓慢沿烧杯壁加入到水相并不断沿同一方向搅拌，速度不宜过快。约30min后，乳化完成即得到O/W型乳液；c) After heating the oil phase and the water phase in a water bath at 75°C for 20 minutes at the same time, slowly add the oil phase to the water phase along the wall of the beaker in a thin stream and keep stirring in the same direction, but the speed should not be too fast. After about 30 minutes, the emulsification is completed and the O/W emulsion is obtained;
d)西兰花汁和豌豆苗汁各5.0ml混匀，上述乳液静置冷却至室温，加入维生素C和烟酰胺各0.5g、再加入混匀的西兰花汁和豌豆苗汁；d) Mix 5.0ml each of broccoli juice and pea sprouts juice, let the above emulsion stand and cool to room temperature, add 0.5g of vitamin C and niacinamide, and then add the mixed broccoli juice and pea sprouts juice;
e)称取10％乙二胺四乙酸二钠溶液0.2g、20％尼泊金甲酯醇溶液0.02mg、量取丙三醇5.0ml，混合均匀，加入上述乳液中，不断搅拌使混合均匀；e) Weigh 0.2g of 10% disodium edetate solution, 0.02mg of 20% methylparaben alcohol solution, and 5.0ml of glycerin, mix them evenly, add them into the above emulsion, and keep stirring to make the mix evenly ;
f)在制得的乳液中加入上述已制备好的卡波姆凝胶和海藻酸钠凝胶，不断搅拌，制得粘度和稠度适宜的乳液。f) Add the prepared carbomer gel and sodium alginate gel into the prepared emulsion, and keep stirring to prepare an emulsion with suitable viscosity and consistency.
实施例3Example 3
实施例1、2的理化指标如下：The physicochemical index of embodiment 1,2 is as follows:
3.1性状3.1 Traits
本品为气味清新自然，质地均匀细腻，粘稠度适宜，涂展性良好而清爽的淡绿色乳液。This product is a light green emulsion with fresh and natural smell, uniform and delicate texture, suitable viscosity, good spreadability and refreshing.
3.2PH值检查3.2PH value check
用玻璃棒蘸取少量乳液于湿润的PH试纸，与标准比色卡对比，PH为6-7。Dip a small amount of emulsion on wet PH test paper with a glass rod, and compare it with the standard color card, the PH is 6-7.
3.3冷热试验3.3 Hot and cold test
将乳液封装于透明化妆品瓶中，于4℃的冰箱冷藏一个月观察无分层现象。于55℃的恒温箱内24h，乳液无分层、变味等现象。The emulsion was packaged in a transparent cosmetic bottle and refrigerated in a refrigerator at 4°C for one month to observe no delamination. In a constant temperature box at 55°C for 24 hours, the emulsion has no phenomenon of stratification and odor change.
3.4离心试验3.4 Centrifugal test
将乳液封装于具塞试管中3000r/min，离心20min，无破乳分层现象。The emulsion was encapsulated in a stoppered test tube at 3000r/min, centrifuged for 20min, and there was no phenomenon of demulsification and stratification.
3.5室温放置试验3.5 Placement test at room temperature
将上述所制的乳液置于化妆品瓶中，室温静置考察6个月，无分层现象，用后感觉无变化、气味无变化。The emulsion prepared above was placed in a cosmetic bottle, and left standing at room temperature for 6 months, no delamination phenomenon, no change in feeling and smell after use.
3.6刺激性试验、过敏性实验3.6 Irritation test, allergy test
小白鼠背部剪去毛，涂抹上述制备的乳液于剪去毛的部位，和没涂部位进行比较，结果没有刺激性和过敏反应。The hair on the back of the mouse was cut off, and the lotion prepared above was applied to the cut off area, and compared with the unpainted area, there was no irritation and allergic reaction.
取上述制备的乳液涂于志愿者(25-65岁，60人)手面上，过30分钟后观察，均没出现发红，发疹，起泡现象。The emulsion prepared above was applied to the hands of volunteers (25-65 years old, 60 people), and observed after 30 minutes, no redness, rash or blistering occurred.
3.7小鼠皮肤组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)、丙二醛(MDA)水平检测3.7 Detection of superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA) levels in mouse skin tissue
SOD作用的重要意义在于清除H2O2和OH·的前身超氧离子，从而保护细胞不受毒性氧自由基的损伤。通过检测其活性反应细胞抗氧化能力；GPX能催化GSH变为GSSG，使有毒的过氧化物还原成无毒的羟基化合物，从而保护细胞膜的结构及功能不受过氧化物的干扰及损害；MDA是自由基引起的脂质过氧化过程中生成的一种醛类物质，通过检测其含量可以反映细胞脂质过氧化的程度。The significance of SOD is to remove superoxide ions, the precursors of H2 O2 and OH·, so as to protect cells from damage by toxic oxygen free radicals. By detecting its activity, it can reflect the antioxidant capacity of cells; GPX can catalyze GSH into GSSG, and reduce toxic peroxides to non-toxic hydroxyl compounds, thereby protecting the structure and function of cell membranes from interference and damage by peroxides; MDA is An aldehyde substance produced during lipid peroxidation caused by free radicals, its content can reflect the degree of cellular lipid peroxidation.
将20只小鼠随机分为两组，实验组、对照组各10只。对照组的处理：将小鼠背部左侧毛剔除，饲养两个月后，取左侧部分皮肤组织用0.86％冷生理盐水机械匀浆制备成10％匀浆液，2500r/min，4℃离心10min后，取上清液测得其中的SOD、GPX和MDA水平；实验组的处理：将小鼠背部左侧毛剔除，将乳液均匀涂抹在去毛皮肤上，每日早晚两次连续涂抹两个月后，与对照组相同处理并比较结果。检测结果见下表1。The 20 mice were randomly divided into two groups, 10 in the experimental group and 10 in the control group. Treatment of the control group: remove the left hair on the back of the mouse, and after two months of feeding, take the left part of the skin tissue and mechanically homogenize it with 0.86% cold saline to prepare a 10% homogenate, centrifuge at 2500r/min, 4°C for 10min Afterwards, the supernatant was taken to measure the levels of SOD, GPX and MDA; the treatment of the experimental group: remove the hair on the left side of the back of the mouse, apply the emulsion evenly on the depilated skin, and apply two consecutive times in the morning and evening every day. After one month, they were treated in the same way as the control group and the results were compared. The test results are shown in Table 1 below.
表1 各指标检测结果Table 1 Test results of each index
+阳性：表示酶的活性升高或含量增加；+ Positive: indicates that the activity or content of the enzyme is increased;
-阴性：表示酶的活性降低或含量减少。- Negative: Indicates that the activity or content of the enzyme is reduced.
实验表明，本乳液显著提高皮肤细胞的SOD、GPX水平并降低MDA含量，其明显的抗氧化损伤作用，说明该乳液有良好的抗皱功效。Experiments show that this emulsion significantly increases the SOD and GPX levels of skin cells and reduces the content of MDA. Its obvious anti-oxidative damage effect shows that the emulsion has a good anti-wrinkle effect.
3.8抗皱效果实验3.8 Anti-wrinkle effect experiment
通过试用实验来评价上述所制乳液的抗皱效果。利用本发明的制备工艺生产出来的乳液与市售抗皱乳液进行对比实验。选取25-55岁女性160名，根据面部细纹的深浅程度，把她们任意等分成4个组，每组40人，每天早晚各两次用乳液涂面部，实验组1为年轻组(25-35岁)，实验组2为年轻组(25-35岁)，实验组3为中年组(35-45岁)，实验组4为老年组(45-55岁)，对照组涂抹市售保湿抗皱乳液，连续使用两个月。用肉眼观察肌肤的改善状况来评价上述所制备乳液抗皱嫩肤效果，结果如下表2。The anti-wrinkle effect of the emulsion prepared above was evaluated through trial experiments. The emulsion produced by the preparation process of the present invention is compared with the commercially available anti-wrinkle emulsion. Select 160 women aged 25-55, divide them into 4 groups according to the depth of facial fine lines, 40 people in each group, apply lotion to the face twice a day in the morning and evening, and the experimental group 1 is the young group (25- 35 years old), experimental group 2 is the young group (25-35 years old), experimental group 3 is the middle-aged group (35-45 years old), experimental group 4 is the elderly group (45-55 years old), the control group is smeared with commercially available moisturizing Anti-wrinkle lotion, used continuously for two months. The improvement of the skin was observed with the naked eye to evaluate the anti-wrinkle and skin rejuvenation effect of the emulsion prepared above. The results are shown in Table 2 below.
表2 使用效果Table 2 Effect of use
3.9该乳液综合效果评价3.9 Evaluation of the comprehensive effect of the emulsion
以下利用试用感觉来评价制造出的乳液的功效。采用民意调查评分法，随机抽取80名25-55岁年龄段的女性作为试用对象，根据不同肤质分组进行考察，将上述制备的乳液搽胳膊。每天使用两次，使用时间两个月。各项使用效果总分为5分：五分为最高分，表示很好，非常满意；4分为较好；3分为可以接受；3分以下为不好，不能接受。以下为各项平均得分。实验结果见表3Next, the effect of the produced emulsion was evaluated by using the trial feeling. Using the public opinion survey scoring method, 80 women aged 25-55 were randomly selected as trial subjects, and were grouped according to different skin types for investigation, and the lotion prepared above was applied to the arms. Use twice a day for two months. The total score of each use effect is 5 points: 5 points are the highest score, which means very good and very satisfied; 4 points are good; 3 points are acceptable; below 3 points are bad and unacceptable. Below are the average scores for each category. The experimental results are shown in Table 3
表3 综合评价结果Table 3 Comprehensive evaluation results
综上所述，本发明所研制的乳液对各种肤质都具有良好的保湿、滋润、抗皱效果，使用后舒适清爽，令皮肤紧致水嫩，此外还有一定祛除色素斑、美白滋养的效果，是一种特别适合较年轻及中年女性肌肤护理和修复的抗皱乳液。In summary, the emulsion developed by the present invention has good moisturizing, moisturizing and anti-wrinkle effects on various skin types. It is comfortable and refreshing after use, making the skin firm and supple. Effect, is an anti-wrinkle lotion especially suitable for skin care and repair of younger and middle-aged women.
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| CN201410424269.2ACN104188859B (en) | 2014-08-26 | 2014-08-26 | A kind of crease-resistant skin care emulsion containing natural component extract and preparation method thereof |
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