The preparation method of Valved bovine jugular veinTechnical field
This patent belongs to the manufacturing technology field using biomaterial for medical purpose, more particularly, to a kind of Valved bovine jugular veinPreparation method.
Background technology
The congenital heart disease incidence of disease occupies the first place of inborn defect.Congenital heart disease there are about 1/3 patient exist different degrees of right ventricle orLeft ventricular outflow tract obstruction, wherein quite a few need to apply valved conduit or Valved allograft piece to rebuild right ventricular outflow canObtain radical cure, as the serious pulmonary stenosis in tetrad of Fallot, double outlet of right ventricle, transposition of great artery, pulmonary atresia or commonWith truncus artiriosus etc..Conventional material has artificial synthesized xenogenesis valved conduit, allogeneic sustainer valved conduit, homograftPipeline, but all there is limitation in these materials, calcification as easy in xenogenesis pipeline, endometrial hyperplasia；Pipeline source of the same race is difficult, implantationStill there is slight rejection, size is difficult to mate when rescuing infant's pathology in host's body.Bovine jugular vein possess abundance,The advantages of length is enough, is presently believed to be a kind of biomaterial very with research future.But with it in manufacturing processHis foreign material is the same, is required for, through fixing process, to increase tissue intensity and durability, reducing antigenicity and immunogeneProperty.The fixing operation of tradition is after the blood vessel clear water of having pruned collection, physiological saline, balanced salt solution solution clean up,It is placed directly in fixer and is fixed, this method, it cannot be guaranteed that valved conduit is fixing fully, cannot ensure to carry lobe pipe simultaneouslyThe original shape in road, is unfavorable for that performs the operation coincide it is impossible to ensure the quality of product, brings risk to patient vitals.
Content of the invention
This patent provides a kind of making of Valved bovine jugular vein for solving technical problem present in known technologyMethod, the method both ensure that extra vascular was fixing and in turn ensure that the natural shape of blood vessel, not fold completely, non-warping knotting,Coincideing beneficial to operation.
This patent is adopted the technical scheme that by solving technical problem present in known technology：
A kind of preparation method of Valved bovine jugular vein, comprises the following steps,
A. select Niu Yuan：2～5 years old ox age, health, free from infection and the cows after butchering are selected to be Niu Yuan；
B. gather blood vessel：Scratch ox neck fat layer with knife and search out jugular vein blood vessel, catch blood vessel knife along blood vesselDraw at heart, the blood vessel taking off proceeded to cleaning in 1 minute；
C. clean：Repeatedly rinsed with clear water, wash color and other pollutant off, the blood vessel cleaned is put into and fills 4 DEG CInterim storage in the ice bucket of～10 DEG C of physiological saline, the time control of storage is in 3 hours；
D. primary election, cleaning：With operating scissors, the fat of blood vessel surface and sheath are cut off, by pathology, the blood that has scarPipe etc. is eliminated；
E. rinse：Balanced salt solution is poured in the container filling blood vessel, is gently agitated for stirring rod, so that blood vessel is uniformly dissipatedOpen, changed a liquid every 1 hour, change balanced salt solution 6 times altogether, then cleaned again with physiological saline, concrete operations are walked with CCleaning part split-phase in rapid is same；
F. fix：First blood vessel side is ligatured with suture, prepare in advance 0.6% glutaraldehyde fixer is filledEnter in tube chamber, after filling, ligature opposite side, then blood vessel is put into fixing in the container filling 0.6% glutaraldehyde fixer, temperatureIt is maintained at 4-12 DEG C；Again blood vessel is transferred in 0.3% glutaraldehyde fixer after 48 hours, fixes one week, standby；
G. bag woven dacron：It is sewn into tubular with the woven dacron that width is 25mm, its internal diameter should be with blood vessel in the shape that naturally relaxesState diameter is identical, and is sleeved on the position of valve of blood vessel, and lower port and blood vessel top layer suture one week around each thereon,Blood vessel can not be pierced through during suture, and keep the natural relaxed state of blood vessel.
This patent can also adopt the following technical scheme that：
Described A selects the step of Niu Yuan to select stable breeding buffalo.
This patent has the advantages and positive effects that：
After this patent employs above technical scheme, reasonably utilize the natural duct structure of bovine jugular vein, by fixerDirectly pour in tube chamber, ligature blood vessel two ends, then bovine jugular vein is put in fixer, both ensure that extra vascular has been fixedEntirely, in turn ensure that the natural shape of blood vessel, not fold, non-warping knotting, coincideing beneficial to operation.Reduce patient suffering and operationThe trouble of person, improves the security of its use, reduces operation risk, and improves procedure efficiency.
Specific embodiment
For content of the invention, feature and effect of this patent can be further appreciated that, hereby enumerate following examples, and describe in detailAs follows：
1. select Niu Yuan：2～5 years old ox age, healthy (outward appearance ox hair is thin, even, shinny), free from infection and the ox after butcheringGroup, kind is preferred with stable breeding buffalo.
2. gather blood vessel：Scratch ox neck fat layer with knife and search out jugular vein blood vessel, catch blood vessel knife along blood vesselDraw at heart, intercept longer portion as far as possible and vascular wall can not be injured, fiber etc. should be avoided during winning in follow-up workIt is difficult the Substances Pollution blood vessel removing, the blood vessel taking off should proceed to cleaning in 1 minute in sequence.
3. clean:Repeatedly rinsed with clear water (no any visible foreign), wash color and other pollutant off, by cleanBlood vessel puts into interim storage in the ice bucket of physiological saline filling 4 DEG C～10 DEG C.The ice bucket filling blood vessel is directly taken back processBetween, the time in way should control in 3 hours.
4. primary election, cleaning：With operating scissors, the fat of blood vessel surface and sheath are cut off.Will be too big for diameter change, sickChange, blood vessel of scar etc. are disposed.
5. rinse：Balanced salt solution is poured in the container filling blood vessel, is gently agitated for stirring rod, so that blood vessel is uniformly dissipatedOpen.Change a liquid every 1 hour, change balanced salt solution 6 times altogether.Then cleaned again with physiological saline.Concrete operations and 3 stepsIn cleaning part split-phase with
6. fixing：First blood vessel side is ligatured with suture, prepare in advance 0.6% glutaraldehyde fixer is filledEnter in tube chamber, after filling, ligature opposite side, then blood vessel is put into fixing in the container filling 0.6 glutaraldehyde fixer, temperature is protectedHold at 4-12 DEG C.Again blood vessel is transferred in 0.3% glutaraldehyde fixer after 48 hours, fixes one week, standby.
7. bag woven dacron：It is sewn into tubular with the woven dacron that width is 25mm, this internal diameter should relaxed naturally with blood vesselState diameter is identical, and is sleeved on the position of valve of blood vessel, and lower port and blood vessel top layer suture one week around each thereon,Certain attention can not pierce through blood vessel, and keeps the natural relaxed state of blood vessel.
Although being described to the preferred embodiment of this patent above, this patent is not limited to above-mentioned concreteEmbodiment, above-mentioned specific embodiment is only schematically, is not restricted, those of ordinary skill in the artUnder the enlightenment of this patent, in the case of without departing from this patent objective and scope of the claimed protection, can also make veryMulti-form, these belong within the protection domain of this patent.