Biological sample processing systemTechnical field
The present invention relates to a kind of biological sample experimental provisions, the automatic drip piece operation suitable for biological sample preparation processTechnical field and cytogenetics experimental technique field.
Background technique
According to classical cytogenetics, the chromosome prepared from cell can be analyzed under the microscope, to possibleExisting defect is diagnosed.Chromosome preparation need to carry out the cell of harvest hypotonic, fixed, dry, aging, enzymic digestion andThe processing such as aobvious band, after these processing, just can under the microscope analyze the chromosome of cell, to judge whether to depositIn chromosome abnormality.This classical method is continued to use always till now at the beginning of foundation.
In the past few decades, Cytogenetic techniques achieve various important breakthroughs, as cell synchronization is trainedThe technology of supporting, chromosome high resolution technology and amniocyte chromosome analysis technology in situ etc..Although in Cytogenetic techniques sideFace has achieved these great breakthroughs, but to reach optimal Chromosome spread degree and its high consistency and repeatabilityIt is still extremely difficult problem, it is difficult to ensure that each as the consistency and again of its Chromosome spread of the sample of cytogeneticRenaturation.
According to Chromosome spread principle of dynamics, in conventional dyeing production procedure, the temperature and humidity pair of environmentThere is very big influence in the quality of chromosome preparation.To solve influence of the environmental factor to Chromosome spread degree, the happy occasion biology in ShanghaiThe Bioexperiment instrument of the entitled Chromprep S of product of Science and Technology Ltd.'s manufacture, the manufacture of Euroclone company, ItalyThe entitled Optichrome Bioexperiment instrument of product, Thermotron Cytogenetic chamber company, the U.S. and JapanHanabin Metaphase spreader company develops manually Chromosome spread instrument respectively, very good solution environmentInfluence of the factor to Chromosome spread, it is ensured that the preparation quality of chromosome.
With increasing for analytical specimen amount, manual Chromosome spread instrument is increasingly unable to satisfy the need of routine analysis workIt asks.Japanese Hanabin company develops automatic staining body drop piece instrument Metaphase Auto-spreader, solves dye substantiallyThe high-throughput problem of colour solid preparation, but the system is a site type machine, and volume is larger, and structure is complicated, and energy consumption is higher, rawProduction, operation and maintenance cost are very high.
Summary of the invention
In order to solve prior art problem, it is an object of the present invention to overcome the deficiencies of the prior art, and to provide one kindBiological sample processing system can carry out the operation of chromosome automatic drip piece with high throughput, small in size, production cost is low, installation andEasy to maintain, low energy consumption.The present invention is especially suitable for CYTOGENETIC ANALYSIS OF ONEs, realize the automation of chromosome drop piece process, mentionHigh analyte efficiency ensure that the quality of biological sample processing.
Purpose is created to reach foregoing invention, the present invention adopts the following technical solutions:
A kind of biological sample processing system, including system backplane, glass slide placement platform, sample cell, biological sample drip pieceDevice and sample treatment chamber, glass slide placement platform are fixedly installed in system backplane, are arranged on glass slide placement platform and are carriedSlide further includes control system and man-machine interface, and control system drips sheet devices by control biological sample, by biology to be processedSample is transferred in slide surface, and user realizes information exchange by man-machine interface and control system, and biological sample drips pieceDevice includes spatial movement mechanism, liquid-transfering device and glass slide fetching device, and the fixed part of spatial movement mechanism passes through bracketIt is fixedly connected with system backplane, the bottom of bracket is fixedly installed in system backplane, the action part difference of spatial movement mechanismIt is fixedly connected with liquid-transfering device and glass slide fetching device, control system makes spatial movement machine by control spatial movement mechanismThe motion profile of the motion parts output setting of structure makes glass slide fetching device to glass slide when biological sample tests beginningAutomatically glass slide is placed on placement platform, and make liquid-transfering device by biological sample from the sample cell of sample cell automatic sucking and fromTurn moves on on the surface of glass slide, after the completion of biological sample processing, removes glass slide automatically from glass slide placement platform；Glass slide placement platform is mounted in sample treatment chamber body, and sample treatment cavity is enclosed by shell cavity with open toppedChamber, glass slide placement platform is mounted at the open topped following position directly of counter sample process chambers, also for sample atReason cavity is specially equipped with setting temperature control equipment, humidity conditioner and air flow controller, real respectively by control systemNow to the automatic control of the inner chamber of sample treatment cavity indoor temperature, humidity and air velocity；Sample cell is provided with temperatureRegulating device, for sample cell for placing biological sample, temperature-adjusting device regulates and controls the temperature in sample cell.
As the improvement of technical solution of the present invention, system further includes label machine and barcode scanner, label machineIt connect with control system signal with barcode scanner, is swept using label of the barcode scanner to biological sample to be processed respectivelyIt reads, and sends scanning information to control system, the setting method and process, transmission that control system is handled according to biological sample are markedPrint command is signed to label machine, prints the label containing sample and glass slide information.
As an improvement of the above technical solution, system further includes exhaust gas adsorption device, by bracket, by exhaust gas adsorption deviceIt is mounted on the top of sample treatment cavity, glass slide placement platform, sample cell and biological sample drop sheet devices, is used for automatic absorbingThe pernicious gas generated in biological sample treatment process.
Above-mentioned exhaust gas adsorption device is preferably made of filter and air exhauster, during realization automatic absorbing BioexperimentThe gas of generation is equipped with adsorbent filter layer in filter, it is preferred to use adsorbent be in soda lime, calcium lime and activated carbonAny one or any several compositions mixture.
The spatial movement mechanism of above-mentioned biological sample drop sheet devices preferably uses XYZ three-shaft linkage device, i.e., by X-axis machineThe controlled motion mechanism composition that space is mutually orthogonal between three structure, Y-axis mechanism and Z-axis mechanism mechanism axis, X-axis mechanism, Y-axisMechanism and Z-axis mechanism are respectively provided with fixed part and moving portion, and wherein the fixed part of X-axis mechanism is fixedly installed in the column of bracketOn, the fixed part of Y-axis mechanism is fixedly installed in the moving portion of X-axis mechanism, and the fixed part of Z-axis mechanism is then fixedly installed in YIn the moving portion of axis mechanism, liquid-transfering device and glass slide fetching device are fixedly installed in the moving portion of Z-axis mechanism, tri- axis of XYZThe moving portion of each controlled motion mechanism of linkage passes through independent motor respectively and carries out corresponding space line displacement movement,Individually under the transmission of the exclusive motor of X-axis mechanism, the fixed part of the moving portion QianYZhou mechanism of X-axis mechanism carries out side-to-side movement,Individually under the transmission of the exclusive motor of Y-axis mechanism, the fixed part of the moving portion traction Z-axis mechanism of Y-axis mechanism carries out front and back fortuneDynamic, individually under the transmission of the exclusive motor of Z-axis mechanism, the moving portion of Z-axis mechanism moves up and down, that is, passes through control systemEach motor of XYZ three-shaft linkage device is controlled, to control liquid-transfering device and glass slide fetching device respectively according to the space of settingPath is mobile, to realize the automatic transporting of glass slide, placement and remove process, and realize the transfer of biological sample, draw andDrop lets off journey.Each motor is stepper motor, direct current generator, DC speed-reducing, step-servo motor and DC servo motor.It is excellentStep-servo motor and DC servo motor are selected, motor operation course can accurately be monitored.Sensor is photoelectric sensingDevice, Hall sensor, capacitance sensor or infrared sensor wait until, can detect to motor movement position.It is preferred that photoelectricitySensor, using with it is easy for installation.X-axis mechanism realizes that side-to-side movement, the realization of Y-axis mechanism move forward and backward, and Z-axis mechanism is realized up and downMovement.
As the improvement of above-mentioned technical proposal of the present invention, preferably by the temperature control equipment and load of above-mentioned sample treatment cavitySlide placement platform constitute sample treatment platform, wherein temperature control equipment preferably by temperature control element, radiating block, temperature-dropping fan andTemperature sensor composition, the temperature of temperature sensor real-time detection glass slide placement platform, control system is according to temperature sensorThe detected value of temperature is compared with setting value, regulates and controls to temperature control element by the temperature signal of acquisition, realizes to glass slideThe control of the temperature of placement platform.Above-mentioned temperature control element preferably uses PTC heating element or semiconductor temperature-control element, and preferably adoptsWith semiconductor temperature-control element, by the heating or cooling of temperature control element, heated up to glass slide placement platform or the control that cools down.
The humidity conditioner and air flow controller of above-mentioned sample treatment cavity are preferably by the outer of sample treatment cavityPortion is integrated into constant humidity air-stream generating device, that is, constant humidity air-stream generating device is a closed cavity, sample treatment chamberBody has air inlet, and the air inlet of constant humidity air-stream generating device passes through defeated with the gas outlet of constant humidity air-stream generating deviceGas flow optimized of the air-stream generating device as sample treatment cavity is arranged in air pipe connection in constant humidity air-stream generating deviceDevice realizes the flow control of delivery air, and air-stream generating device preferably uses blower or air pump, and constant humidity air-flow fillsSet mainly by humidification water pond, water heating elements, water temperature sensor, condenser, dehumidifying semiconductor, heat-conducting block, cooling fan andTemperature Humidity Sensor composition, control system control water heating elements carry out heating manufacture water to the water body stored in humidification water pond and steamGas, water temperature sensor real-time detection humidify the water temperature in water pond, temperature detection value and setting of the control system according to waterThe comparison result of value, regulates and controls water heating elements, realizes that outside air is in air-stream generating device to the accuracy controlling of water temperatureDriving under, into the inside cavity of constant humidity air-stream generating device, flow through the surface of the water body stored in humidification water pond, takeThe humidified rear pipeline for entering condenser with vapor, then exsiccation region and temperature and humidity followed by dehumidifying semiconductorThe measurement point of sensor is finally flowed out from gas outlet, and into sample treatment inside cavity, cooling fan is by heat-conducting block to dehumidifyingSemiconductor is cooled down, and the heat for generating dehumidifying semiconductor successively can be discharged into environment through heat-conducting block and cooling fan, is controlledThe detected value of temperature and humidity is compared, to dehumidifying by the temperature-humidity signal that system is acquired according to Temperature Humidity Sensor with setting valueThe working condition of semiconductor is regulated and controled, and realizes the humid control of the air-flow to the exsiccation region for flowing through dehumidifying semiconductor.
Above-mentioned water heating elements preferably use PTC heating element or semiconductor temperature-control element, pass through adding for water heating elementsHeat, control flow through the humidity of the air-flow of humidification water panel surface.
As the improvement of above-mentioned technical proposal of the present invention, be preferably fixedly mounted on above system bottom plate glass slide heap frame andGlass slide layer frame, when biological sample, which is tested, to be started, control slide fetching device automatically picks up glass slide from glass slide heap frameIt takes, the open topped after carrying again by sample treatment cavity places glass slide into the inside of sample treatment cavityTo above slide platforms, and after the completion of biological sample experiment, control slide fetching device is automatically by glass slide from glass slideIt is picked up on platform, passes through the open topped of sample treatment cavity again after carrying, leave sample treatment cavity, by glass slide pointIt is not placed into the corresponding layer frame of glass slide layer frame.
As the improvement of above-mentioned technical proposal of the present invention, controllable temperature sample cell is set in system backplane, controllable temperature sampleThe temperature-adjusting device in pond includes temperature sensor, temperature controller, radiator and cooling blower, the sample cell case of controllable temperature sample cellBody top is equipped with the sunk type sample well of multiple openings, biological sample is placed in sunk type sample well, temperature sensor is real-timeThe internal temperature of test sample pond cabinet, the temperature signal that control system is acquired according to temperature sensor, by the detected value of temperatureIt is compared with setting value, temperature controller is regulated and controled, realize the internal temperature control to sample cell cabinet.
Above-mentioned temperature controller preferably uses PTC heating element or semiconductor temperature-control element, and preferred semiconductor temperature control element passes throughThe heating or cooling of temperature controller, heat up to the intracorporal sample of sample cell case or the control that cools down.
As the improvement of above-mentioned technical proposal of the present invention, pipette tips box and de- pipette tips device, biological sample are equipped in system backplaneThe action part energy automatic loading and unloading that product drip the spatial movement mechanism of sheet devices is loaded pipette tips, and control system passes through control spatial movementMechanism, realize automatic sampling gun take arms automatically head, take off pipette tips, mixed liquid automatically and add imbibition operation, sample loading gun includes motor, pistonAnd pipette tips, electric machine main shaft is by driving piston up-down to carry out imbibition and filling operation.
The motor of above-mentioned sample loading gun preferably uses stepper motor, direct current generator or decelerating motor.
Above-mentioned glass slide fetching device preferably includes negative pressure pump, pressure sensor and glass slide suction nozzle, control system according toThe pressure signal of pressure sensor acquisition, the detected value of pressure is compared with setting value, is regulated and controled to negative pressure pump, is realizedThe spatial movement mechanism that sheet devices include is dripped to the work method control of glass slide suction nozzle, and by linkage biological sample, is now carriedSlide takes being automatically brought into operation for piece and film releasing.
Above-mentioned control system is preferably using any one in dynamic control card, single-chip microcontroller, computer, programmable controllerKind.
The present invention compared with prior art, has following obvious prominent substantive distinguishing features and remarkable advantage:
1. the constant humidity air-stream generating device of biological sample processing system of the present invention, can accurately control at sampleThe intracorporal temperature of chamber, humidity and air velocity are managed, the samples such as peripheral blood, amniotic fluid, marrow or chorion after fixer is fixedPneumatic conveying drying is stabilized in sample treatment cavity of the invention, cell fixer is volatilized with speed appropriate, be can controlChromosome spread process significantly improves metaphase chromosome number, guarantees optimal Chromosome spread degree and Chromosome spreadHigh consistency and repeatability；
2. the constant humidity air-stream generating device that the present invention uses, humid control precision is high, small in size, simplifies wellThe internal structure of system, realizes the miniaturization of system；
3. biological sample processing box of the present invention is equipped with gas filtration absorption device, can be with decontamination of biological sample preparation procedureThe pernicious gases such as the methanol and acetic acid of middle generation greatly reduce harmful substance and break to the injury of operator and to environmentIt is bad；
4. low energy consumption by the present invention, compared with similar products, energy consumption can be reduced to 500W from 1200W；
5. the XYZ three-shaft linkage that the present invention uses, the automatic drop piece operation for carrying out sample, high degree of automation, efficiencyIt is high；
6. biological sample processing system of the present invention is easy to operate, low manufacture cost is had good market prospects.
Detailed description of the invention
Fig. 1 is the biological sample processing system overall appearance structural representation of the embodiment of the present invention one.
Fig. 2 is the structural schematic diagram of the sample treatment cavity of the embodiment of the present invention one.
Fig. 3 is the structural schematic diagram of the constant humidity air-stream generating device of the embodiment of the present invention three.
Fig. 4 is the structural schematic diagram of the controllable temperature sample cell of the embodiment of the present invention four.
Specific embodiment
Details are as follows for the preferred embodiment of the present invention:
Embodiment one:
In the present embodiment, it is placed referring to Fig. 1, a kind of biological sample processing system, including system backplane 7, glass slide flatPlatform 21, sample cell 6, biological sample drop sheet devices and sample treatment cavity, glass slide placement platform 21 are fixedly installed in system bottomOn plate 7, glass slide 25 is set on glass slide placement platform 21, further includes control system 15 and man-machine interface 16, control system15 be single-chip microcontroller, and control system 15 drips sheet devices by control biological sample, biological sample to be processed is transferred to glass slideOn 25 surfaces, user realizes information exchange, man-machine interface 16 and control system 15 by man-machine interface 16 and control system 15It is connected by communication wire, is set by parameter of the man-machine interface 16 to sample handling processes, according to specific sampleProcessing requirement changes the method and process of sample treatment, by man-machine interface 16, while show sample treatment procedure information,The status information of sensor information and associated control modules, it includes spatial movement mechanism, liquid-transfering device 8 that biological sample, which drips sheet devices,It is fixedly connected by bracket 14 with system backplane 7 with the fixed part of glass slide fetching device 9, spatial movement mechanism, bracket 14Bottom be fixedly installed in system backplane 7, the action part of spatial movement mechanism is picked and placed with liquid-transfering device 8 and glass slide respectivelyDevice 9 is fixedly connected, and control system 15 makes the motion parts of spatial movement mechanism export setting by control spatial movement mechanismMotion profile, biological sample test start when, place glass slide fetching device 9 automatically on glass slide placement platform 21Glass slide 25, and make liquid-transfering device 8 by biological sample from 6 automatic sucking of sample cell and be automatically transferred to the surface of glass slide 25On, after the completion of biological sample processing, remove glass slide 25 automatically from glass slide placement platform 21；Glass slide placement platform 21It being mounted in sample treatment cavity 2, sample treatment cavity 2 is enclosed the chamber with open topped 29 by shell cavity 28,Glass slide placement platform 21 is mounted at the open topped following position directly of counter sample process chambers 2, is also sample treatment chamberBody 2 is specially equipped with setting temperature control equipment, humidity conditioner and air flow controller, is realized respectively by control system 15Automatic control to the inner chamber of sample treatment cavity 2 indoor temperature, humidity and air velocity；Sample cell 6 is provided with temperatureRegulating device, for sample cell 6 for placing biological sample, temperature-adjusting device regulates and controls the temperature in sample cell 6.
In the present embodiment, referring to Fig. 1, system further includes label machine 17 and barcode scanner 18, label machine17 and barcode scanner 18 connect respectively with 15 signal of control system, using barcode scanner 18 to the mark of biological sample to be processedLabel carry out sweeping reading, and send scanning information to control system 15, the setting method that control system 15 is handled according to biological sampleAnd process, label print command is sent to label machine 17, prints the label containing sample and glass slide information.In this implementationIn example, referring to Fig. 1, label machine 17 and barcode scanner 18 are connected by controlling route with control system 15, are to controlSystem 15 sends and receivees instruction, is read out using label information of the barcode scanner 18 to sample to be processed, and is with controlSystem 15 is communicated, and according to the setting method and process of sample treatment, abstract factory printer 17 prints to be contained control system 15The label of the information such as sample and glass slide is realized that the glass slide 25 after sample treatment is corresponding with processing sample, is avoided outExisting mistake.
In the present embodiment, referring to Fig. 1, system further includes exhaust gas adsorption device 19, by bracket 14, exhaust gas is adsorbed and is filled19 tops for being mounted on sample treatment cavity, glass slide placement platform 21, sample cell 6 and biological sample drop sheet devices are set, are used forThe pernicious gas generated in automatic absorption biological sample treatment process.
In the present embodiment, referring to Fig. 1, the spatial movement mechanism that biological sample drips sheet devices is XYZ three-shaft linkage device,By space between 10 3 X-axis mechanism 3, Y-axis mechanism 4 and Z-axis mechanism mechanism axis, mutually orthogonal controlled motion mechanism is formed,X-axis mechanism 3, Y-axis mechanism 4 and Z-axis mechanism 10 are respectively provided with fixed part and moving portion, wherein the fixed peace of the fixed part of X-axis mechanism 3On column loaded on bracket 14, the fixed part of Y-axis mechanism 4 is fixedly installed in the moving portion of X-axis mechanism 3, and Z-axis mechanism 10Fixed part is then fixedly installed in the moving portion of Y-axis mechanism 4, and liquid-transfering device 8 and glass slide fetching device 9 are fixedly installed in Z axisIn the moving portion of mechanism 10, the moving portion of each controlled motion mechanism of XYZ three-shaft linkage device pass through respectively independent motor intoThe corresponding space line displacement movement of row, individually under the transmission of the exclusive motor of X-axis mechanism 3, the moving portion of X-axis mechanism 3 leads YThe fixed part of axis mechanism 4 carries out side-to-side movement, individually under the transmission of the exclusive motor of Y-axis mechanism 4, the moving portion of Y-axis mechanism 4The fixed part of traction Z-axis mechanism 10 is moved forward and backward, individually under the transmission of the exclusive motor of Z-axis mechanism 10, Z-axis mechanism 10Moving portion move up and down, i.e., by control system 15 control XYZ three-shaft linkage device each motor, to control shifting respectivelyLiquid device 8 and glass slide fetching device 9 are mobile according to the space path of setting, to realize the automatic transporting of glass slide 25, putProcess is set and removed, and realizes that journey is let off in the transfer, absorption and drop of biological sample.
In the present embodiment, referring to Fig. 1, it is fixedly arranged above bracket 14 in XYZ three-shaft linkage device, X-axis mechanism 3 is fixed onOn the column of bracket 14, one end of 4 mechanism of Y-axis is fixed in X-axis mechanism 3.Z-axis mechanism 10 is fixed in Y-axis mechanism 4.XYZ axisUnder motor drive, it is driven by screw rod, under the transmission of the exclusive motor of X-axis mechanism, Y-axis mechanism carries out left and right fortuneDynamic, under the transmission of the exclusive motor of Y-axis mechanism, Z-axis mechanism carries out the movement of front and back, in the biography of the exclusive motor of Z-axis mechanismUnder dynamic, liquid-transfering device 8 and glass slide fetching device 9 move up and down.XYZ three-shaft linkage realizes glass slide 25 and wait locateSample is managed in the movement of three-dimensional space, and realizes the automation of sample handling processes.
In the present embodiment, referring to Fig. 1 and Fig. 2, glass slide heap frame 13 and glass slide layer are fixedly mounted in system backplane 7Frame 11, when biological sample, which is tested, to be started, control slide fetching device 9 automatically picks up glass slide 25 from glass slide heap frame 13It takes, passes through the open topped 29 of sample treatment cavity 2 again after carrying, into the inside of sample treatment cavity 2, by glass slide25 are placed into above slide platforms 21, and after the completion of biological sample experiment, slide fetching device 9 is controlled automatically by glass slide25 pick up from slide platforms 21, pass through the open topped 29 of sample treatment cavity 2 again after carrying, leave sample treatmentGlass slide 25 is placed individually into the corresponding layer frame of glass slide layer frame 11 by cavity 2, and by open topped 29, liquid-transfering device 8 will25 surface of glass slide is drawn from sample cell 6 and be added to processing sample, carries out decentralized processing.
In the present embodiment, referring to Fig. 1 and Fig. 2, the temperature control equipment of sample treatment cavity 2 and glass slide placement platform21 constitute sample treatment platform, and wherein temperature control equipment is by temperature control element 22, radiating block 23, temperature-dropping fan 24 and temperature sensingDevice 27 forms, and temperature control element 22 is installed among slide platforms 21 and radiating block 23, and 27 real-time detection of temperature sensor carries glassThe temperature of piece placement platform 21, the temperature signal that control system 15 is acquired according to temperature sensor 27, by the detected value of temperature withSetting value is compared, and is regulated and controled to temperature control element 22, is realized according to the platform temperature of setting to glass slide placement platform 21Temperature control.
In the present embodiment, referring to fig. 2, temperature control element 22 is semiconductor temperature-control element, passes through the heating of temperature control element 22Or cooling, it is heated up to glass slide placement platform 21 or the control that cools down.
In the present embodiment, it is equipped with pipette tips box 5 referring to Fig. 1, in the system backplane 7 inside sample treatment cavity 2 and takes offPipette tips device 12, the action part energy automatic loading and unloading that biological sample drips the spatial movement mechanism of sheet devices are loaded pipette tips, control system15, by control spatial movement mechanism, realize automatic sampling gun, head of taking arms automatically, automatically de- pipette tips and the operation of mixed liquid, sample loading gunIncluding motor, piston and pipette tips, the motor of sample loading gun uses stepper motor, and electric machine main shaft is by driving piston up-down to carry outImbibition and filling operation.
In the present embodiment, it referring to Fig. 1, is operated according to the drop piece of chromosome in classical cytogenetics, passes through tri- axis of XYZLinkage, is sequentially completed following movement:
1. glass slide 25 is first taken from glass slide heap frame 13 and is put on sample treatment cavity 2 by glass slide fetching device 9In glass slide placement platform 21 on；
2. liquid-transfering device 8 pipettes liquid feeding pipette tips；
3. the sample loading gun of liquid-transfering device 8 carries out sample mixing operation；
4. sample loading gun pipette samples；
5. the sample liquid feeding that liquid-transfering device 8 draws sample loading gun to be located at sample treatment chamber body 2 in 25 surface of glass slide；
6. the sample on 25 surface of glass slide volatilizees under conditions of setting temperature and humidity, chromosome is dispersed；
7. after sample treatment, glass slide fetching device 9 will be picked and placed containing the glass slide 25 for having handled sample to load glassIn piece layer frame 11, a sample treatment circulation is completed, then carries out new sample treatment circulate operation.
In the present embodiment, referring to Fig. 1 and Fig. 2, biological sample processing means, including constant humidity air-stream generating device 1,Liquid-transfering device 8, glass slide fetching device 9, sample cell 6, exhaust gas adsorption device 19, XYZ three-axis moving device, barcode scanner18, label machine 17, glass slide heap frame 13, glass slide layer frame 11, pipette tips box 5 and control system 15.Liquid-transfering device 8 is by sampleAfter sample blending in pipe, it is added drop-wise to 25 surface of glass slide and is handled.Glass slide fetching device 9 is by glass slide 25 from glass slideIt picks and places on heap frame 13 into sample treatment cavity 2, is handled, handled, then glass slide 25 is picked and placed and arrives glass slide layer frame 11On.XYZ three-axis moving device controls liquid-transfering device 8 and glass slide fetching device 9 along the movement in three X-axis, Y-axis and Z axis directionsOperation.Three parts are linked, and that completes sample automatically processes process.The embodiment biological sample processing means are particularly suitableIn CYTOGENETIC ANALYSIS OF ONE, realizes the automation of chromosome drop piece process, improve analysis efficiency, guarantee the quality of sample treatment.Barcode scanner 18 is used to scan the bar code on sample cell, and control system 15 believes the bar code information of sample cell, sample cell positionBreath, final glass slide location information printed by label machine 17, be attached on glass slide 25.
Embodiment two:
The present embodiment is basically the same as the first embodiment, and is particular in that:
In the present embodiment, exhaust gas adsorption device 19 is made of filter and air exhauster, realizes that automatic absorbing biology is realThe gas generated during testing, filter is interior to be equipped with adsorbent filter layer, and the adsorbent used is soda lime, exhaust gas adsorption device19 are located at the top of sample processing system, are made of blower, adsorbent and shell.It is generally solid through methanol and acetic acid to handle sampleSurely the cell suspension handled is dripped containing volatile organic solvents such as methanol and acetic acid in the sample of piece processing, during processingVolatile methanol and acetic acid escaping gas can be generated, internal system is discharged into, device itself, operator and environment are depositedIt is adversely affecting.Air containing escaping gas is pumped into exhaust gas adsorption device by the blower of exhaust gas adsorption device 19Portion flows through the surface of adsorbent, the pernicious gases such as methanol and acetic acid in adsorbent adsorption gas flow, then gives off clean gasStream, realizes the purpose of air cleaning, the escaping gas that can effectively generate in adsorption sample treatment process, eliminate its to environment andThe harm of operator's health.Air-flow inside device is pumped into adsorbent equipment by blower, and filtered air-flow can be discharged to dressSet outside.
Embodiment three:
The present embodiment is substantially the same as in the previous example, and is particular in that:
In the present embodiment, referring to Fig. 3, the humidity conditioner and air flow controller of sample treatment cavity 2 are by sampleThe external harmoniousness of product process chambers 2 is constant humidity air-stream generating device 1, and constant humidity air-stream generating device 1 is one closedCavity, sample treatment cavity 2 have air inlet 26, the air inlet 26 and constant humidity air-flow of constant humidity air-stream generating device 1The gas outlet 61 of generating device 1 is connected to by air delivering pipeline, and air-stream generating device is arranged in constant humidity air-stream generating device 160 realize the flow control of delivery air as the air flow controller of sample treatment cavity 2, and air-stream generating device 60 is air pump,Constant humidity air-stream generating device 1 mainly by humidification water pond 51, water heating elements 53, water temperature sensor 54, condenser 55, removeWet semiconductor 58, heat-conducting block 56, cooling fan 57 and Temperature Humidity Sensor 59 form, and control system 15 controls water heating elements 53Heating manufacture vapor is carried out to the water body 52 stored in humidification water pond 51,54 real-time detection of water temperature sensor humidifies water pond 51In 52 temperature of water body, control system 15 is according to the temperature detection value of water and the comparison result of setting value, to water heating elements 53Regulated and controled, realize the accurate control of water temperature, outside air is under the driving of air-stream generating device 60, into constant humidity air-flowThe air-flow for the specified moisture that constant humidity air-stream generating device 1 generates is imported by the inside cavity of generating device 1, the present embodimentIn sample treatment cavity 2, optimal environmental condition is provided for sample treatment, it is ensured that the processing quality of sample flows through humidification water pondThe surface of the water body 52 stored in 51 carries vapor and the humidified rear pipeline for entering condenser 55, is condensed out partial moisture,To realize the regulation to airflow humidity, then exsiccation region and Temperature Humidity Sensor 59 followed by dehumidifying semiconductor 58Measurement point, finally flowed out from gas outlet 61, into inside sample treatment cavity 2, cooling fan 57 is by heat-conducting block 56 to removingWet semiconductor 58 is cooled down, and the heat for generating dehumidifying semiconductor 58 is successively discharged into ring through heat-conducting block 56 and cooling fan 57In border, it is ensured that the lasting normal work of the balance of heat and the semiconductor 58 that dehumidifies, control system 15 is according to Temperature Humidity Sensor 59The Temperature and Humidity result of air-flow is sent control system 15 by the temperature-humidity signal of acquisition, by the detected value of temperature and humidity with setDefinite value is compared, and is regulated and controled to the working condition of dehumidifying semiconductor 58, is realized and is made to the dehumidifying for flowing through dehumidifying semiconductor 58With the humid control of the air-flow in region, to accurately control the humidity of effluent stream, it is ensured that constant humidity air-stream generating device 1 producesThe humidity of raw air-flow is within the set range.
In the present embodiment, referring to Fig. 3, water heating elements 53 are semiconductor heating element, pass through water heating elements 53Heating, control flow through the humidity of the air-flow on humidification 51 surface of water pond.Water after heating plays humidification to the air-flow for being pumped into cavity and makeesWith, by airflow humidity increase to setting humidity on.
Example IV:
The present embodiment is substantially the same as in the previous example, and is particular in that:
In the present embodiment, referring to fig. 4, controllable temperature sample cell 6 is set in system backplane 7, the temperature of controllable temperature sample cell 6Spending regulating device includes temperature sensor 69, temperature controller 67, radiator 66 and cooling blower 65, the sample of controllable temperature sample cell 6Cabinet 68 top in pond is equipped with the sunk type sample well 70 of multiple openings, for placing biological sample to be processed, in sunk type sampleBiological sample, the temperature of the biological sample of the inside of 69 real-time detection sample cell cabinet 68 of temperature sensor, control are placed in hole 70The temperature signal that system 15 processed is acquired according to temperature sensor 69, the detected value of temperature is compared with setting value, to temperature controlDevice 67 is regulated and controled, and is realized and is controlled the temperature of sample cell cabinet 68.The heat generated in 67 course of work of temperature controller is through radiatingBody 66 and blower 65 can be discharged into environment, it is ensured that the balance of heat and the lasting normal work of temperature control element.In the present embodiment,Referring to fig. 4, temperature controller 67 is semiconductor temperature-control element, and temperature controller 67 is installed among sample cell cabinet 68 and radiator 66, is led toThe heating or cooling for crossing temperature controller 67, heat up to the sample in sample cell cabinet 68 or the control that cools down.
Embodiment five:
The present embodiment is substantially the same as in the previous example, and is particular in that:
In the present embodiment, glass slide fetching device 9 includes negative pressure pump, pressure sensor, glass slide suction nozzle, control system15 pressure signals acquired according to pressure sensor, the detected value of pressure is compared with setting value, is adjusted to negative pressure pumpControl realizes the work method control to glass slide suction nozzle, and drips the space that sheet devices include by linkage control biological sample and transportMotivation structure, existing glass slide 25 take being automatically brought into operation for piece and film releasing.
The embodiment of the present invention is illustrated above in conjunction with attached drawing, but the present invention is not limited to the above embodiments, it can be withThe purpose of innovation and creation according to the present invention makes a variety of variations, under the Spirit Essence and principle of all technical solutions according to the present inventionThe changes, modifications, substitutions, combinations, simplifications done should be equivalent substitute mode, as long as meeting goal of the invention of the invention, onlyOtherwise the technical principle and inventive concept for deviating from biological sample processing system of the present invention, belong to protection scope of the present invention.