技术领域technical field
本发明涉及血液分离装置，更具体地涉及一种通过离心分离而提取PRP的血液分离装置。The present invention relates to a blood separation device, more particularly to a blood separation device for extracting PRP through centrifugation.
背景技术Background technique
血液由血浆、血小板及血细胞构成，其中，血小板包含人体组织再生和修复所需的成分。对血液进行离心分离时，根据其重量，分离为血浆、血小板及血细胞，按此方法分离的血小板形成为血浆丰富的血浆，即富血小板血浆(PRP:Platelet Rich Plasma)和缺乏血浆的血小板，即贫血小板血浆(PPP:Platelet Poor Plasma)。因血小板含有各种生长因子，对创伤治愈和皮肤再生具有重要的作用。报告显示，PRP以高浓度含有该血小板，由此，各种成长因子促进周围细胞的繁殖，具有促进充分地合成胶原蛋白等成分的效果。由此，最近在腰痛等病痛的治疗、脱发的治疗、皮肤再生或烧伤治疗等皮肤疾病等各种领域使用PRP。Blood is composed of plasma, platelets and blood cells, among which platelets contain the components needed for the regeneration and repair of human tissues. When blood is centrifuged, it is separated into plasma, platelets and blood cells according to its weight. The platelets separated by this method are formed into plasma rich in plasma, that is, platelet rich plasma (PRP: Platelet Rich Plasma) and platelets lacking in plasma, namely Platelet Poor Plasma (PPP: Platelet Poor Plasma). Because platelets contain various growth factors, they play an important role in wound healing and skin regeneration. Reports have shown that PRP contains the platelets in a high concentration, thereby promoting the proliferation of surrounding cells by various growth factors, and has the effect of promoting sufficient synthesis of components such as collagen. As a result, PRP has recently been used in various fields such as the treatment of ailments such as low back pain, the treatment of hair loss, skin regeneration, and skin diseases such as burn treatment.
具有将通过用于分离该PRP的方法抽取的血液进行离心分离，对血细胞成分和贫血小板血浆(PPP，Platelet Poor Plasma)进行分离，并再次对其进行离心分离，而只提取PRP的方法。但利用现有的血液分离装置的分离方法，存在如下问题，采血之后，在移动至离心分离容器的过程、移动至包含过滤单元的分离容器的过程中，血液会暴露于空气中。并且，利用现有的血液分离装置，难以将血小板浓缩至高浓度，并且，难以准确抽取所需的PRP量。There is a method of centrifuging the blood drawn by the method for separating the PRP, separating blood cell components and platelet poor plasma (PPP, Platelet Poor Plasma), and centrifuging them again to extract only the PRP. However, the separation method using the conventional blood separation device has the problem that after blood collection, the blood is exposed to the air during the process of moving to the centrifuge container and the process of moving to the separation container including the filter unit. Furthermore, it is difficult to concentrate the platelets to a high concentration with the existing blood separation device, and it is difficult to accurately extract the required amount of PRP.
现有专利文献有韩国公开专利文献第10-2011-0045980号。Existing patent documents include Korean Laid-Open Patent Document No. 10-2011-0045980.
发明内容Contents of the invention
技术课题technical issues
本发明是为了解决上述现有技术问题而研发，本发明的目的为提供一种在将血液不暴露至空气的状态下，可以进行两次离心分离的血液分离装置。并且，本发明的目的为提供一种更多地浓缩PRP而能够容易地进行分离的血液分离装置。而且，本发明的目的为提供一种由一个组件准确抽取所需的量的血液分离装置。The present invention is developed to solve the above-mentioned problems in the prior art, and the purpose of the present invention is to provide a blood separation device that can perform two centrifugation without exposing the blood to the air. Furthermore, an object of the present invention is to provide a blood separation device capable of concentrating more PRP and easily separating it. Furthermore, it is an object of the present invention to provide a blood separation device that accurately draws a required amount with one unit.
技术方案Technical solutions
一种血液分离装置，其特征在于，包括：第1组件，内侧包括容纳血液的第1空间，所述第1空间通过第1开口部上下分离；第2组件，内侧包括容纳血液的第2空间，所述第2空间通过第2开口部上下分离；盘，配置于所述第1组件及所述第2组件之间，具有使所述第1空间和所述第2空间进行流体连通的连通孔；限位器，具有：第1杆(bar)，从所述盘向上侧突出配置，以开闭所述第1开口部，及第2杆，从所述盘向下侧突出配置，以开闭所述第2开口部；第1盖子，遮盖所述第1组件的上端部；及第2盖子，遮盖所述第2组件的下端部，其中，所述限位器通过分别开闭所述第1开口部和所述第2开口部，而进行多次血液分离。A blood separation device, characterized in that it includes: a first assembly, the inside of which includes a first space for containing blood, and the first space is separated up and down by a first opening; a second assembly, inside of which includes a second space for containing blood , the second space is separated up and down by the second opening; the disk is disposed between the first assembly and the second assembly, and has a communication link for fluid communication between the first space and the second space The hole; the stopper has: a first rod (bar) protruding upward from the disk to open and close the first opening, and a second rod protruding downward from the disk to opening and closing the second opening; a first cover covering the upper end of the first component; and a second cover covering the lower end of the second component, wherein the stoppers are opened and closed respectively The first opening and the second opening are used to perform multiple blood separations.
本发明的一实施例，所述第1空间及所述第2空间在注入血液之前，保持真空状态。According to an embodiment of the present invention, the first space and the second space are kept in a vacuum state before blood is injected.
本发明的一实施例，所述限位器与所述第1组件及所述第2组件通过螺丝可旋转地结合，并上下移动。According to an embodiment of the present invention, the stopper is rotatably combined with the first component and the second component by screws, and moves up and down.
所述第1组件或所述第2组件具有用于注入或抽取血液的开口部。The first unit or the second unit has an opening for injecting or drawing blood.
本发明的一实施例，所述开口部具有防污用过滤单元。In one embodiment of the present invention, the opening has an antifouling filter unit.
本发明的一实施例，所述第1盖子与所述第1组件通过螺丝可旋转地结合，并上下移动。In one embodiment of the present invention, the first cover and the first assembly are rotatably coupled with screws, and move up and down.
本发明的一实施例，所述第2盖子与所述第2组件通过螺丝可旋转地结合，并上下移动。In one embodiment of the present invention, the second cover is rotatably coupled to the second assembly by screws, and moves up and down.
本发明的一实施例，所述第1盖子或所述第2盖子包括硅胶或橡胶材质的部件。According to an embodiment of the present invention, the first cover or the second cover includes parts made of silicone or rubber.
本发明的一实施例，所述第1组件、所述第2组件、所述限位器、所述第1盖子及所述第2盖子中的一个以上由透明的材质形成。In an embodiment of the present invention, at least one of the first component, the second component, the stopper, the first cover, and the second cover is formed of a transparent material.
本发明的一实施例，所述第1盖子或所述第2盖子标注有刻度线。According to an embodiment of the present invention, the first cover or the second cover is marked with scale marks.
本发明的一实施例，所述第1组件或所述第2组件为分离成上侧空间及下侧空间的沙漏状。In one embodiment of the present invention, the first unit or the second unit is in an hourglass shape separated into an upper space and a lower space.
本发明的一实施例，在所述限位器和所述第1组件之间，及所述限位器和所述第2组件之间配置有硅胶或橡胶材质的密封圈。According to an embodiment of the present invention, sealing rings made of silica gel or rubber are disposed between the limiter and the first component, and between the limiter and the second component.
有益效果Beneficial effect
根据本发明，能够提供一种在将血液不暴露至空气的状态下，可以进行两次离心分离的血液分离装置。并且，能够提供一种更多地浓缩PRP而能够容易地进行分离的血液分离装置。而且，能够提供一种由一个组件准确抽取所需的量的血液分离装置。According to the present invention, it is possible to provide a blood separation device capable of performing centrifugation twice without exposing blood to air. In addition, it is possible to provide a blood separation device capable of concentrating more PRP and easily separating it. Furthermore, it is possible to provide a blood separation device that accurately draws a required amount with one unit.
本发明的效果并非限定于所述效果，应当理解，包括从本发明的详细的说明或权利要求书中记载的发明的结构中推论出的所有效果。The effects of the present invention are not limited to the above-described effects, and should be understood to include all effects deduced from the detailed description of the present invention or the structure of the invention described in the claims.
附图说明Description of drawings
图1为本发明的一实施例的血液分离装置的剖视图；Fig. 1 is a cross-sectional view of a blood separation device according to an embodiment of the present invention;
图2为图1的血液分离装置的分解剖视图；Fig. 2 is an exploded cross-sectional view of the blood separation device in Fig. 1;
图3及图4为图1的血液分离装置的截面图；3 and 4 are cross-sectional views of the blood separation device of FIG. 1;
图5为示意显示图1的血液分离装置的限位器的运行的部分截面图；Fig. 5 is a partial cross-sectional view schematically showing the operation of the stopper of the blood separation device of Fig. 1;
图6为显示防污用过滤单元的配置形式的部分剖视图；Fig. 6 is a partial sectional view showing the configuration of the antifouling filter unit;
图7及图8为显示本发明的一实施例的血液分离装置的实际形状的附图。7 and 8 are drawings showing the actual shape of a blood separation device according to an embodiment of the present invention.
具体实施方式Detailed ways
下面，参照附图对本发明进行说明。但本发明以各种不同的形式实现，因此，并非限定于在此说明的实施例。并且为了在附图中明确说明本发明，省略了与说明无关的部分，并且，通过整个说明书对相似的部分标注了相似的附图符号。Hereinafter, the present invention will be described with reference to the accompanying drawings. However, the present invention can be realized in various forms, and therefore is not limited to the embodiments described here. In addition, in order to clarify the present invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are assigned to similar parts throughout the specification.
在整个说明书中，某一部分与另一部分"连接"时，包含"直接连接"的情况，也包括其中间隔着其它部件并"间接连接"的情况。并且，某一部分“包含”某一构成要素时，无特别反对性记载的，并非排出其它构成要素，还包括另一构成要素。Throughout the specification, when a certain part is "connected" to another part, the case of "direct connection" is included, and the case of "indirect connection" with other parts interposed therebetween is also included. In addition, when a certain part "contains" a certain constituent element, if there is no particularly objectionable statement, it does not exclude other constituent elements, but also includes another constituent element.
参照下面附图，对本发明的实施例进行详细说明。Embodiments of the present invention will be described in detail with reference to the following drawings.
图1为本发明的一实施例的血液分离装置的剖视图，图2为图1的血液分离装置的分解剖视图，图3及图4为图1的血液分离装置的截面图，图5为示意显示图1的血液分离装置的限位器的运行的部分截面图，图6显示防污用过滤单元的配置形式的部分剖视图。Fig. 1 is a cross-sectional view of a blood separation device according to an embodiment of the present invention, Fig. 2 is an exploded cross-sectional view of the blood separation device in Fig. 1, Fig. 3 and Fig. 4 are cross-sectional views of the blood separation device in Fig. 1, and Fig. 5 is a schematic display Fig. 1 is a partial cross-sectional view showing the operation of the stopper of the blood separation device, and Fig. 6 is a partial cross-sectional view showing the configuration of the anti-fouling filter unit.
参照图1及图2,血液分离装置1包括第1盖子10、第1组件20、限位器30、第2组件40及第2盖子50。根据此构成要素，共形成用于血液分离的3个腔(空间部)，此类腔分别相互开放或关闭。Referring to FIGS. 1 and 2 , the blood separation device 1 includes a first cover 10 , a first component 20 , a stopper 30 , a second component 40 and a second cover 50 . According to this constituent element, three cavities (space portions) for blood separation are formed in total, and these cavities are mutually opened or closed.
参照图3及图4，对各个构成要素进行详细地说明。首先，第1盖子10遮盖第1组件20的上端部。第1盖子10包括：外侧部11，接触于下面所述的第1组件20的上侧圆筒部21的外径；及内侧部12，固定结合于该外侧部11，并接触于第1组件20的上侧圆筒部21的内径。Each component will be described in detail with reference to FIGS. 3 and 4 . First, the first cover 10 covers the upper end of the first unit 20 . The first cover 10 includes: an outer portion 11, which is in contact with the outer diameter of the upper cylindrical portion 21 of the first component 20 described below; and an inner portion 12, which is fixedly coupled to the outer portion 11, and is in contact with the first component. The inner diameter of the upper cylindrical portion 21 of 20.
第1盖子10的外侧部11的内径具有螺旋部13，该螺旋部13与位于下面所述的第1组件20的上侧圆筒部21的螺旋部26通过螺丝可旋转地结合。相对于第1组件20，通过旋转第1盖子10，第1盖子10相对于第1组件20可进行上下移动。由此，能够调节容纳于第1组件20的内侧空间(第1空间)的血液的量。The inner diameter of the outer portion 11 of the first cap 10 has a screw portion 13 rotatably coupled to a screw portion 26 located on an upper cylindrical portion 21 of the first unit 20 described below by screws. With respect to the first assembly 20 , by rotating the first cover 10 , the first cover 10 can move up and down relative to the first assembly 20 . Thereby, the amount of blood accommodated in the inner space (first space) of the first unit 20 can be adjusted.
第1盖子10的内侧部12固定结合于外侧部11。例如，如图3所示，位于外侧部11的侧面内周面的突出部对准插入位于内侧部13的侧面外周面的凹槽，内侧部12和外侧部11进行固定结合。The inner portion 12 of the first cover 10 is fixedly coupled to the outer portion 11 . For example, as shown in FIG. 3 , the protrusions on the inner surface of the outer side 11 are aligned and inserted into the grooves on the outer side surface of the inner side 13 , and the inner side 12 and the outer side 11 are fixedly combined.
内侧部12为具有柔韧性的材质，例如硅胶或橡胶材质的部件。通过内侧部12插入注射针,可以将血液注入血液分离装置1的内部,也可以从血液分离装置1抽取完成分离的血液。因内侧部12由具有柔韧性的材质形成，从而，内侧部12能够紧密结合于第1组件20的上侧圆筒部21。由此，在第1盖子10和第1组件20之间，即使没有另外的密封部件，也能密封以防止流体渗漏。The inner portion 12 is made of a flexible material, such as silicone or rubber. Inserting the injection needle through the inner part 12 can inject blood into the blood separation device 1 , and can also extract the separated blood from the blood separation device 1 . Since the inner portion 12 is formed of a flexible material, the inner portion 12 can be tightly coupled to the upper cylindrical portion 21 of the first assembly 20 . Thereby, between the 1st cover 10 and the 1st assembly 20, even if there is no separate sealing member, it can seal and prevent fluid leakage.
第1组件20配置于第1盖子10的下侧，其内侧包含容纳血液的第1空间。第1组件20包括：上侧圆筒部21；下侧圆筒部22，一体连接于该上侧圆筒部21；内侧圆锥部23，在上侧圆筒部21及下侧圆筒部22之间，向内侧突出形成；颈部24，形成于内侧圆锥部23的上侧。颈部24具有第1开口部24a，借助该第1开口部24a，第1空间上下分离为上侧空间20a和下侧空间20b。The first unit 20 is disposed on the lower side of the first cover 10, and the inside thereof includes a first space for containing blood. The first assembly 20 includes: an upper cylindrical portion 21; a lower cylindrical portion 22 integrally connected to the upper cylindrical portion 21; an inner conical portion 23 between the upper cylindrical portion 21 and the lower cylindrical portion 22. between, protruding inward; the neck 24 is formed on the upper side of the inner conical portion 23 . The neck portion 24 has a first opening 24a, and the first space is vertically divided into an upper space 20a and a lower space 20b by means of the first opening 24a.
上侧圆筒部21的外侧面的上端部具有与上述第1盖子10的螺旋部13结合的上侧螺旋部26。下侧圆筒部22的外侧面的下端部具有与下面所述的限位器30的上侧螺旋部35结合的下侧螺旋部27。对下侧圆筒部22和限位器30之间的螺丝结合说明如下。The upper end portion of the outer surface of the upper cylindrical portion 21 has an upper spiral portion 26 coupled to the spiral portion 13 of the first cap 10 described above. The lower end portion of the outer surface of the lower cylindrical portion 22 has a lower helical portion 27 coupled to an upper helical portion 35 of a stopper 30 described below. The screw connection between the lower cylindrical portion 22 and the stopper 30 will be described below.
内侧圆锥部23在上侧圆筒部21和下侧圆筒部22接触的部位上，趋向内侧空间(第1空间)，形成越至上侧，直径渐小的圆锥状。内侧圆锥部23的上端具有向上侧方向延伸形成的管状的颈部24。通过此颈部24，第1空间分离为上下侧的同时，进行血液离心分离时，血液的构成成分发生自由上下移动。并且，血液的离心分离结束时，通过关闭该颈部24，使血液进行空间分离。The inner conical portion 23 is formed in a conical shape whose diameter gradually decreases toward the upper side toward the inner space (first space) at the portion where the upper cylindrical portion 21 and the lower cylindrical portion 22 are in contact. The upper end of the inner conical portion 23 has a tubular neck portion 24 extending upward. The neck portion 24 separates the first space into the upper and lower sides, and when the blood is centrifuged, the components of the blood freely move up and down. Then, when the centrifugation of blood is completed, the neck 24 is closed to spatially separate the blood.
在内侧圆锥部23的侧面部具有供注入或抽取血液的开口部25。该开口部25紧密配置有未图示的盖子。此盖由硅胶或橡胶材质构成，由此将注射器内的血液注入至血液分离装置1内，或将完成分离的血液通过注射器抽取至外部。An opening 25 through which blood is injected or drawn is provided on the side surface of the inner conical portion 23 . A cover (not shown) is closely arranged on the opening 25 . The cover is made of silica gel or rubber material, so as to inject the blood in the syringe into the blood separation device 1 or extract the separated blood to the outside through the syringe.
另外，开口部25具有防污用过滤单元。参照图6，开口部25紧密设置有防污用过滤单元251，过滤单元251上固定结合有密封圈252。并且密封圈252上具有开口部盖子253。由此,血液分离装置1内的真空状态被破坏时，打开开口部盖子253，并通过过滤单元251，在血液分离装置1内可进行空气连通的状态下，通过下面所述的其它开口部46，将血液注入血液分离装置1内，或将完成分离的血液抽取至外部。限位器30配置于第1组件20和第2组件40之间。限位器30包括：外侧圆筒部31；盘32，位于外侧圆筒部31的内径中央部；第1杆(bar)33，从盘32的中央部上面向上侧突出设置至上侧；第2杆34，从盘32的中央部下面向下侧突出设置至下侧。In addition, the opening 25 has an antifouling filter unit. Referring to FIG. 6 , an antifouling filter unit 251 is closely arranged in the opening 25 , and a sealing ring 252 is fixedly combined with the filter unit 251 . In addition, the sealing ring 252 has an opening cover 253 . Thus, when the vacuum state in the blood separation device 1 is broken, open the opening cover 253, and pass through the filter unit 251, and then pass through the other openings 46 described below in the state where air can be communicated in the blood separation device 1. , to inject blood into the blood separation device 1 , or to extract the separated blood to the outside. The stopper 30 is disposed between the first component 20 and the second component 40 . The limiter 30 includes: an outer cylindrical portion 31; a disk 32 located at the center of the inner diameter of the outer cylindrical portion 31; a first rod (bar) 33 protruding upward from the upper surface of the central portion of the disk 32 to the upper side; The rod 34 protrudes downward from the lower surface of the central portion of the disk 32 .
在外侧圆筒部31的内侧面的上侧具有与上述第1组件20的下侧螺旋部27结合的上侧螺旋部35。由此，限位器30和第1组件20可旋转地通过螺丝进行结合，相对于限位器30，使第1组件20进行上下移动，借此能够开闭第1组件20的第1开口部24a。An upper spiral portion 35 coupled to the lower spiral portion 27 of the first unit 20 is provided on the upper side of the inner surface of the outer cylindrical portion 31 . Thereby, the stopper 30 and the first assembly 20 are rotatably coupled by screws, and the first opening of the first assembly 20 can be opened and closed by moving the first assembly 20 up and down relative to the stopper 30 24a.
外侧圆筒部31的内侧面的下侧具有与下文所述的第2组件40的上侧螺旋部47结合的下侧螺旋部36。由此，限位器30和第2组件40通过螺丝可旋转地结合，相对于限位器30，使第2组件40进行上下移动，借此可开闭第2组件40的第2开口部43a。The lower side of the inner surface of the outer cylindrical portion 31 has a lower spiral portion 36 coupled to an upper spiral portion 47 of the second unit 40 described below. As a result, the stopper 30 and the second assembly 40 are rotatably coupled by screws, and the second assembly 40 moves up and down relative to the stopper 30, whereby the second opening 43a of the second assembly 40 can be opened and closed. .
参照图5，相对于限位器30，使第1组件20向上侧移动，由此开启第1组件20的第1开口部24a，并且相对于限位器30，使第2组件40向下侧移动，由此确认开启第2组件40的第2开口部43a的状态。Referring to FIG. 5 , relative to the stopper 30 , the first assembly 20 is moved upward to thereby open the first opening 24 a of the first assembly 20 , and the second assembly 40 is moved downward relative to the stopper 30 . The second opening 43 a of the second assembly 40 is opened by moving the second assembly 40 .
再次参照图3及图4，在限位器30和第1组件20之间配置有密封圈60。该密封圈60为硅胶或橡胶材质的部件，借此密封防止流体渗漏至限位器30和第1组件20之间。Referring to FIGS. 3 and 4 again, a seal ring 60 is disposed between the stopper 30 and the first assembly 20 . The sealing ring 60 is made of silicone or rubber, so as to seal and prevent fluid from leaking between the limiter 30 and the first assembly 20 .
并且，在限位器30和第2组件40之间配置有密封圈70。此密封圈70为硅胶或橡胶材质的部件，借此密封防止流体渗漏至限位器30和第2组件40之间。Furthermore, a seal ring 70 is disposed between the stopper 30 and the second assembly 40 . The sealing ring 70 is made of silicone or rubber, so as to seal and prevent fluid from leaking between the limiter 30 and the second component 40 .
盘32为薄板状，该盘32具有上下贯通的连通孔32a。俯视时，连通孔32a为球形、扇状或半月形，但并非限定于此形状。借助连通孔32a，上述第1组件20的内侧空间(第1空间)和下文所述的第2组件40的内侧空间(第2空间)进行流体连通。即，第1组件20的下侧空间20b实际上与下文所述的第2组件40的上侧空间40a作用为一体。The disk 32 has a thin plate shape, and the disk 32 has a communication hole 32a penetrating up and down. The communication hole 32a is spherical, fan-shaped, or half-moon-shaped when viewed from above, but is not limited to this shape. Through the communication hole 32a, the inner space (first space) of the above-mentioned first module 20 and the inner space (second space) of the second module 40 described later are in fluid communication. That is, the lower space 20b of the first unit 20 is actually integrated with the upper space 40a of the second unit 40 described below.
第1杆33及第2杆34为直径既定长圆柱形的部件，但并非限定于此形状。第1杆33和第2杆34的端部接触于第1开口部24a和第2开口部43a，防止血液流动，为了起到防止该血液流动的功能，第1杆33和第2杆34的端部形成为半球形状。The first rod 33 and the second rod 34 are members having a long cylindrical shape with a predetermined diameter, but are not limited to this shape. The ends of the first rod 33 and the second rod 34 are in contact with the first opening 24a and the second opening 43a to prevent blood from flowing. The end portion is formed in a hemispherical shape.
第2组件40配置于限位器30的下侧，其内侧包含容纳血液的第2空间。第2组件40具有：上侧圆筒部41；上侧连接部42，一体连接于该上侧圆筒部41，且直径趋向下侧渐渐变小；管状的颈部43，一体连接于该上侧连接部42的下端部；下侧连接部44，一体连接于该颈部43，直径向下侧渐渐变大；下侧圆筒部45，一体连接于下侧连接部44。颈部43具有第2开口部43a，借助该第2开口部43a，第2空间上下分离为上侧空间40a和下侧空间40b。即，第2组件40形成为分离成上侧空间40a和下侧空间40b的沙漏状。The second unit 40 is disposed below the stopper 30, and includes a second space inside the stopper 30 for containing blood. The second assembly 40 has: an upper cylindrical portion 41; an upper connecting portion 42 integrally connected to the upper cylindrical portion 41, and the diameter gradually becomes smaller toward the lower side; a tubular neck 43 integrally connected to the upper The lower end portion of the side connecting portion 42 and the lower connecting portion 44 are integrally connected to the neck portion 43 and gradually increase in diameter downward; the lower cylindrical portion 45 is integrally connected to the lower connecting portion 44 . The neck portion 43 has a second opening 43a, and the second space is vertically separated into an upper space 40a and a lower space 40b through the second opening 43a. That is, the second unit 40 is formed in an hourglass shape separated into an upper space 40a and a lower space 40b.
上侧圆筒部41的外侧面的上端部具有与上述限位器30的螺旋部36结合的上侧螺旋部47。下侧圆筒部45的外侧面的下端部具有与下文所述的第2盖子50的螺旋部52结合的下侧螺旋部48。对下侧圆筒部45和第2盖子50之间通过螺丝进行的结合说明如下。The upper end portion of the outer surface of the upper cylindrical portion 41 has an upper helical portion 47 coupled to the helical portion 36 of the stopper 30 described above. The lower end portion of the outer surface of the lower cylindrical portion 45 has a lower spiral portion 48 coupled to a spiral portion 52 of a second cap 50 described later. The coupling between the lower cylindrical portion 45 and the second cover 50 with screws will be described below.
并且，通过第2组件40的颈部43，第2空间分离为上下侧的同时，血液离心分离时，血液的构成成分发生自由上下移动。并且，血液的离心分离完成时，通过关闭颈部43，使血液进行空间性地分离。Furthermore, while the second space is separated into the upper and lower sides by the neck portion 43 of the second unit 40, the constituent components of the blood freely move up and down when the blood is centrifuged. Then, when the centrifugal separation of blood is completed, the blood is spatially separated by closing the neck portion 43 .
上侧连接部42的侧面部具有用于注入或抽取血液的开口部46。此开口部46紧密设置有未图示的盖子。此盖由硅胶或橡胶材质构成，由此将注射器内的血液注入血液分离装置1内，或将完成分离的血液通过注射器，抽取至外部。The side surface of the upper connecting portion 42 has an opening 46 for injecting or drawing blood. This opening portion 46 is tightly provided with an unillustrated cover. The cover is made of silica gel or rubber, so that the blood in the syringe is injected into the blood separation device 1 , or the separated blood is drawn to the outside through the syringe.
第2盖子50位于第2组件40的下侧，以遮盖第2组件40的下端部。第2盖子50包括接触于上述第2组件40的下侧圆筒部45的外径的外侧部51。The second cover 50 is located on the lower side of the second assembly 40 to cover the lower end of the second assembly 40 . The second cover 50 includes an outer portion 51 that is in contact with the outer diameter of the lower cylindrical portion 45 of the second assembly 40 .
第2盖子50的外侧部51的内径具有螺旋部52，该螺旋部52与位于上述第2组件40的下侧圆筒部45的螺旋部48通过螺丝可旋转地结合。相对于第2组件40旋转第2盖子50，由此，第2盖子50相对于第2组件40可上下移动。借此能够调节容纳于第2组件40的内部空间部的血液量。例如，图3中，第2盖子50为相对于第2组件40向下侧移动的状态，由此，下侧空间40b的体积变大，图4中，第2盖子50为相对于第2组件40向上侧移动的状态，由此，下侧空间40b的体积变小。The inner diameter of the outer portion 51 of the second cover 50 has a screw portion 52 rotatably coupled to the screw portion 48 located on the lower cylindrical portion 45 of the second unit 40 by screws. By rotating the second cover 50 relative to the second assembly 40 , the second cover 50 can move up and down relative to the second assembly 40 . Thereby, the amount of blood accommodated in the inner space of the second unit 40 can be adjusted. For example, in FIG. 3 , the second cover 50 is in a state of moving downward relative to the second assembly 40, whereby the volume of the lower space 40b becomes larger. In FIG. 4 , the second cover 50 is relative to the second assembly 40. 40 moves upward, whereby the volume of the lower space 40b becomes smaller.
第2盖子50和第2组件40之间配置有密封圈80。该密封圈80为硅胶或橡胶材质的部件，借此，密封避免流体渗漏至第2盖子50和第2组件40之间。A seal ring 80 is arranged between the second cover 50 and the second assembly 40 . The sealing ring 80 is made of a silicone or rubber material, thereby sealing and preventing fluid from leaking between the second cover 50 and the second assembly 40 .
由此,血液分离装置1划分为3个空间部，即第1组件20的上侧空间20a、第2组件40的下侧空间40b、及一体的空间部即第1组件20的下侧空间20b和第2组件40的上侧空间40a。在此，第1组件20的上侧空间20a和下侧空间20b借助第1开口部24a分离，第2组件40的上侧空间40a和下侧空间40b借助第2开口部43a分离。并且，限位器30通过分别开闭第1开口部24a和第2开口部43a，进行多次血液分离。对此作如下说明。Thus, the blood separation device 1 is divided into three space parts, that is, the upper space 20a of the first assembly 20, the lower space 40b of the second assembly 40, and the lower space 20b of the first assembly 20 as an integral space. And the upper side space 40a of the second assembly 40 . Here, the upper space 20a and the lower space 20b of the first unit 20 are separated by the first opening 24a, and the upper space 40a and the lower space 40b of the second unit 40 are separated by the second opening 43a. Furthermore, the stopper 30 separates the blood multiple times by opening and closing the first opening 24a and the second opening 43a respectively. This is explained as follows.
并且，第1组件20内部的第1空间和第2组件40内部的第2空间在注入血液之前，保持真空状态。第1空间及第2空间保持为真空状态,由此，将血液供给至血液分离装置1时，防止因内部空气压力而发生难以注入的情况。并且，在血液注入时，无需制造外部空气循环通道，因此结构变得简单，使血液的注入变得容易。Furthermore, the first space inside the first unit 20 and the second space inside the second unit 40 are kept in a vacuum state until blood is injected. The first space and the second space are maintained in a vacuum state, whereby when blood is supplied to the blood separation device 1 , it is prevented from being difficult to inject due to internal air pressure. In addition, since there is no need to create an external air circulation channel during blood injection, the structure becomes simple and the blood injection becomes easy.
而且，制造之后，经过一段时间时，血液分离装置1内的真空慢慢被破坏。由此，在维持血液分离装置1内的真空时，在开启某一个开口部25的状态下，也可以通过另外一个开口部46注入血液。Furthermore, after a period of time after manufacture, the vacuum in the blood separation device 1 is gradually broken. Thereby, while the vacuum in the blood separator 1 is maintained, blood can be injected through the other opening 46 even when one of the openings 25 is opened.
并且,血液分离装置1的各个构成要素，即第1盖子10、第1组件20、限位器30、第2组件40、及第2盖子50中的一个以上由透明材料形成。由此，使用者能够容易地观察血液的量或分离状态。Furthermore, one or more of the constituent elements of the blood separation device 1 , that is, the first cover 10 , the first unit 20 , the stopper 30 , the second unit 40 , and the second cover 50 are formed of a transparent material. Thereby, the user can easily observe the amount of blood or the state of separation.
另外，第1盖子10或第2盖子50上标注有刻度线，由此能够准确测量或调整第1组件20的上侧空间20a及第2组件40的下侧空间40b的体积。In addition, the scale marks are marked on the first cover 10 or the second cover 50 , so that the volumes of the upper space 20 a of the first unit 20 and the lower space 40 b of the second unit 40 can be accurately measured or adjusted.
另外，上述实施例中，以第1组件20及第2组件40全部具有用于注入或抽取血液的开口部25、46进行了说明，也可以只具有该开口部25、46中的某一个。并且，也可以只在第1组件20或第2组件40中的某一个上，具有2个以上的开口部。In addition, in the above-mentioned embodiment, the first unit 20 and the second unit 40 are described as having the openings 25, 46 for injecting or drawing blood, but only one of the openings 25, 46 may be provided. In addition, only one of the first module 20 and the second module 40 may have two or more openings.
并且，上述实施例中，以第1组件20和第2组件40形状为各不相同的进行了说明，但第1组件20和第2组件40的形状也可以相同，并且，其形状也可相对。例如，第1组件20也可以形成为沙漏状。And, in the above-mentioned embodiment, the shape of the first component 20 and the second component 40 is different for description, but the shape of the first component 20 and the second component 40 can also be the same, and their shapes can also be opposite. . For example, the first module 20 may be formed in an hourglass shape.
并且，上述实施例中，以第1盖子10和第2盖子50的形状各不相同进行了说明，但第1盖子10和第2盖子50的形状也可以相同，并且，其形状也可以相对。例如，第2盖子50也可以具有硅胶或橡胶材质的部件。In addition, in the above-mentioned embodiment, the shapes of the first cover 10 and the second cover 50 are different, but the shapes of the first cover 10 and the second cover 50 may be the same, and the shapes may be opposite. For example, the second cover 50 may have a silicone or rubber material.
并且，上述实施例中，以外侧圆筒部31的内侧面分别设置有上侧螺旋部35和下侧螺旋部36进行了说明，但外侧圆筒部32的上侧螺旋部35和下侧螺旋部36的某一个，或两个都设置于外侧圆筒部32的外侧面。此情况第1组件20的下侧螺旋部27和第2组件40的上侧螺旋部47的位置，也必须变更地能够与外侧圆筒部31的上侧螺旋部35和下侧螺旋部36结合的位置。In addition, in the above-mentioned embodiment, the upper spiral portion 35 and the lower spiral portion 36 are respectively provided on the inner surface of the outer cylindrical portion 31 for description, but the upper spiral portion 35 and the lower spiral portion of the outer cylindrical portion 32 One or both of the portions 36 are provided on the outer surface of the outer cylindrical portion 32 . In this case, the positions of the lower helical portion 27 of the first assembly 20 and the upper helical portion 47 of the second assembly 40 must also be changed so that they can be combined with the upper helical portion 35 and the lower helical portion 36 of the outer cylindrical portion 31. s position.
图7及图8为本发明的一实施例的血液分离装置1的实际形状的附图。参照图7，能够确认通过血液分离装置1进行血液的离心分离的状态，参照图8，能够确认将血液注入血液分离装置1的状态。7 and 8 are drawings showing the actual shape of the blood separation device 1 according to an embodiment of the present invention. Referring to FIG. 7 , the state of centrifugation of blood by the blood separation device 1 can be confirmed, and referring to FIG. 8 , the state of blood injection into the blood separation device 1 can be confirmed.
下面，利用本发明的一实施例的血液分离装置1，对进行两次血液离心分离的方法进行说明。Next, using the blood separation device 1 according to an embodiment of the present invention, a method of performing two centrifugation of blood will be described.
首先，将抽取的血液通过第1组件20的开口部25或第2组件40的开口部46注入至血液分离装置1内。其次，第1组件20的第1开口部24a借助限位器30的第1杆33关闭，第2组件40的第2开口部43a在开启的状态下，进行第1次离心分离。完成第一次离心分离时，血液中的血浆及血细胞层位于第2组件40的上侧空间40a，血细胞位于下侧空间40b。此时，通过旋转第2盖子50，适当调节下侧空间40b的体积，由此，将血液成分进行正确分离。之后，借助限位器30的第2杆34，关闭第2组件40的第2开口部43a。然后,翻转血液分离装置1，即第1组件20相对于第2组件40，位于下侧之后，在开启第1组件20的第1开口部24a的状态下，进行第2次离心分离。借此，富血小板血浆(PRP)和贫血小板血浆(PPP)分层。对位于下侧的第1盖子10进行旋转，适当调节第1组件20的上侧空间20a的体积之后，相对于第1组件20旋转限位器30，由此，关闭第1组件20的第1开口部24a。按此方式，通过第1盖子10的内侧部12并利用注射器抽取分离的血液中的PRP。First, the drawn blood is injected into the blood separation device 1 through the opening 25 of the first unit 20 or the opening 46 of the second unit 40 . Next, the first opening 24a of the first unit 20 is closed by the first rod 33 of the stopper 30, and the second opening 43a of the second unit 40 is opened to perform the first centrifugation. When the first centrifugation is completed, the plasma and blood cell layers in the blood are located in the upper space 40a of the second unit 40, and the blood cells are located in the lower space 40b. At this time, by rotating the second cover 50, the volume of the lower space 40b is appropriately adjusted, thereby accurately separating the blood components. Thereafter, the second opening 43 a of the second unit 40 is closed by the second lever 34 of the stopper 30 . Then, after the blood separation device 1 is turned over so that the first unit 20 is positioned below the second unit 40, the second centrifugation is performed with the first opening 24a of the first unit 20 opened. Thereby, platelet-rich plasma (PRP) and platelet-poor plasma (PPP) are stratified. Rotate the first cover 10 located on the lower side, after properly adjusting the volume of the upper space 20a of the first assembly 20, then rotate the stopper 30 relative to the first assembly 20, thereby closing the first assembly 20 of the first assembly 20. The opening part 24a. In this manner, the PRP in the separated blood is drawn through the inner portion 12 of the first cap 10 with a syringe.
由此，因血液能够不从血液分离装置10内暴露至外部地进行第2次离心分离，由此，不存在因血液暴露至空气而发生感染的担忧，能够抽取高浓缩的PRP。As a result, the blood can be centrifuged for the second time without being exposed from the inside of the blood separation device 10 to the outside, and thus highly concentrated PRP can be extracted without fear of infection due to exposure of the blood to the air.
上述本发明的说明用于例示，本发明所属技术领域的普通技术人员应当理解，在不变更本发明技术思想或必要特征的情况下，可通过其它具体形式进行变形。因此上述实施例在所有方面，只用于例示，而并非限定本发明。例如，以单一形式说明的各个构成要素进行分开实施，同样地，分开说明的构成要素也能够以结合的形式进行实施。The above description of the present invention is for illustration purposes, and those skilled in the art of the present invention should understand that it can be modified in other specific forms without changing the technical idea or essential features of the present invention. Therefore, the above-mentioned embodiments are only for illustration in all respects, and do not limit the present invention. For example, each constituent element described in a single form can be implemented separately, and similarly, constituent elements described separately can also be implemented in a combined form.
本发明的范围根据权利要求显示，应当理解：从权利要求范围的意义及范围和同等概念进行的各种变更或变形的形式，均包含于本发明的范围内。The scope of the present invention is shown by the claims, and it should be understood that various changes or modifications based on the meaning and scope of the claims and equivalent concepts are included in the scope of the present invention.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2011-0138589 | 2011-12-20 | ||
| KR1020110138589AKR101170028B1 (en) | 2011-12-20 | 2011-12-20 | Blood separating apparatus |
| PCT/KR2012/001235WO2013094819A1 (en) | 2011-12-20 | 2012-02-20 | Blood separating device |
| Publication Number | Publication Date |
|---|---|
| CN104010672Atrue CN104010672A (en) | 2014-08-27 |
| CN104010672B CN104010672B (en) | 2016-01-13 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280063492.8AActiveCN104010672B (en) | 2011-12-20 | 2012-02-20 | blood separation device |
| Country | Link |
|---|---|
| KR (1) | KR101170028B1 (en) |
| CN (1) | CN104010672B (en) |
| WO (1) | WO2013094819A1 (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106040444A (en)* | 2015-04-09 | 2016-10-26 | 韩基洙 | Blood separating vial |
| CN108025320A (en)* | 2015-07-17 | 2018-05-11 | 统计诊断与创新有限公司 | Methods and devices for sample separation and collection |
| CN108992979A (en)* | 2018-07-19 | 2018-12-14 | 中国科学院东北地理与农业生态研究所 | A kind of mariages mouth combination unit of reagent exchange stored filter |
| CN110026297A (en)* | 2018-01-12 | 2019-07-19 | 瑞崴制造股份有限公司 | Body fluid separation device |
| CN111194241A (en)* | 2017-09-05 | 2020-05-22 | 拜克门寇尔特公司 | Collecting and preparing blood samples for immediate diagnosis |
| CN111254045A (en)* | 2018-11-30 | 2020-06-09 | 瑞崴制造股份有限公司 | Kit for separating and concentrating humoral cells containing stem cells |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101626373B1 (en)* | 2015-02-04 | 2016-06-01 | 주식회사 지에스엠코리아 | Blood separating device |
| CN106669875B (en)* | 2017-02-13 | 2022-07-01 | 深圳市邦沃科技有限公司 | Platelet-rich plasma centrifuge tube and extraction method thereof |
| KR102119835B1 (en)* | 2017-11-22 | 2020-06-05 | (주)시지바이오 | Centrifuge device for separating blood components |
| US20210291162A1 (en)* | 2018-04-25 | 2021-09-23 | Global Life Sciences Solutions Usa Llc | Storage Vial |
| KR101892197B1 (en)* | 2018-06-20 | 2018-08-28 | (주)웰포트 | Apparatus for extracting platelet rich plasma |
| KR102527628B1 (en)* | 2019-05-20 | 2023-05-03 | (의) 삼성의료재단 | Container for tissue adhesiveness |
| KR102339809B1 (en)* | 2021-03-30 | 2021-12-16 | (주)레보메드 | Bio kit for extracting bodily fluids containing stem cells |
| KR102395059B1 (en)* | 2021-12-30 | 2022-05-09 | (주) 레보메드 | Internal sterile treatment type body fluid cell separation and concentration kit |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4131549A (en)* | 1977-05-16 | 1978-12-26 | Ferrara Louis T | Serum separation device |
| CN2429826Y (en)* | 2000-05-29 | 2001-05-09 | 北京伏尔特技术有限公司 | Blood separator |
| CN1441690A (en)* | 2000-04-28 | 2003-09-10 | 丰收技术股份有限公司 | Blood components separator disc |
| CN1822903A (en)* | 2003-05-19 | 2006-08-23 | 丰收技术股份有限公司 | Method and apparatus for separating liquid components |
| WO2011071353A2 (en)* | 2009-12-07 | 2011-06-16 | Jeon Min-Yong | Centrifuge tube |
| US20110281714A1 (en)* | 2005-02-07 | 2011-11-17 | Biomet Biologics, Llc | Method and Apparatus for Preparing Platelet Rich Plasma and Concentrates Thereof |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008104789A (en)* | 2006-10-27 | 2008-05-08 | Nipro Corp | Method and device for separation of platelet rich plasma |
| KR101110576B1 (en)* | 2009-03-16 | 2012-02-15 | 주식회사 메디사랑 | Separation and concentration vessel for blood extraction and filler extraction and fibrin production |
| KR101008583B1 (en) | 2010-10-27 | 2011-01-17 | 주식회사 메디사랑 | Container for separating autologous platelet concentrate |
| KR101026599B1 (en) | 2010-12-30 | 2011-04-04 | 문상호 | Container for separating autologous platelet concentrate |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4131549A (en)* | 1977-05-16 | 1978-12-26 | Ferrara Louis T | Serum separation device |
| CN1441690A (en)* | 2000-04-28 | 2003-09-10 | 丰收技术股份有限公司 | Blood components separator disc |
| CN2429826Y (en)* | 2000-05-29 | 2001-05-09 | 北京伏尔特技术有限公司 | Blood separator |
| CN1822903A (en)* | 2003-05-19 | 2006-08-23 | 丰收技术股份有限公司 | Method and apparatus for separating liquid components |
| US20110281714A1 (en)* | 2005-02-07 | 2011-11-17 | Biomet Biologics, Llc | Method and Apparatus for Preparing Platelet Rich Plasma and Concentrates Thereof |
| WO2011071353A2 (en)* | 2009-12-07 | 2011-06-16 | Jeon Min-Yong | Centrifuge tube |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106040444A (en)* | 2015-04-09 | 2016-10-26 | 韩基洙 | Blood separating vial |
| CN108025320A (en)* | 2015-07-17 | 2018-05-11 | 统计诊断与创新有限公司 | Methods and devices for sample separation and collection |
| US10919040B2 (en) | 2015-07-17 | 2021-02-16 | Stat-Diagnostica & Innovation, S.L. | Method for sample separation and collection |
| CN111194241A (en)* | 2017-09-05 | 2020-05-22 | 拜克门寇尔特公司 | Collecting and preparing blood samples for immediate diagnosis |
| US11666917B2 (en) | 2017-09-05 | 2023-06-06 | Beckman Coulter, Inc. | Collection and preparation of blood samples for point-of-care diagnostics |
| CN110026297A (en)* | 2018-01-12 | 2019-07-19 | 瑞崴制造股份有限公司 | Body fluid separation device |
| CN110026297B (en)* | 2018-01-12 | 2021-06-01 | 瑞崴制造股份有限公司 | Body fluid separation device |
| CN108992979A (en)* | 2018-07-19 | 2018-12-14 | 中国科学院东北地理与农业生态研究所 | A kind of mariages mouth combination unit of reagent exchange stored filter |
| CN108992979B (en)* | 2018-07-19 | 2020-09-15 | 中国科学院东北地理与农业生态研究所 | A dual-wire port combination device for reagent exchange, storage and filtration |
| CN111254045A (en)* | 2018-11-30 | 2020-06-09 | 瑞崴制造股份有限公司 | Kit for separating and concentrating humoral cells containing stem cells |
| CN111254045B (en)* | 2018-11-30 | 2023-07-11 | 瑞崴制造股份有限公司 | Body fluid cell separation and concentration kit containing stem cells |
| Publication number | Publication date |
|---|---|
| WO2013094819A1 (en) | 2013-06-27 |
| KR101170028B1 (en) | 2012-08-01 |
| CN104010672B (en) | 2016-01-13 |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address | Address after:Gyeonggi Do, South Korea Patentee after:Ruiwei Medical Equipment Co.,Ltd. Country or region after:Republic of Korea Address before:Gyeonggi Do, South Korea Patentee before:REV-MED, Inc. Country or region before:Republic of Korea | |
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information | Inventor after:Huang Shixuan Inventor after:Shen Fenggen Inventor before:Huang Shixian Inventor before:Shen Fenggen | |
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information | Inventor after:Huang Chouyong Inventor after:Shen Fenggen Inventor before:Huang Shixuan Inventor before:Shen Fenggen |