CN103837463A - Liquid way buffer type sheath fluid steady flow system - Google Patents

Abstract

The invention relates to a liquid way buffer type sheath fluid steady flow system. A direct-through air bag type pulse buffer comprises a cylindrical shell and a compressible telescopic air bag arranged in the shell; an output port of a peristaltic pump is communicated with an inlet of the compressible telescopic air bag by a front liquid flow conduit, and an outlet of the compressible telescopic air bag is communicated with a streaming pool by a rear liquid flow conduit; a closed space is formed between the outer wall of the compressible telescopic air bag and the inner wall of the cylindrical shell, and gas is filled into the space. The direct-through air bag type pulse buffer is arranged, so that the liquid flow pulsation is greatly reduced, and the sheath fluid can run more steadily; the length of a pipeline does not need to be prolonged, so that the liquid way buffer type sheath fluid steady flow system is small in design volume and space-saving for the low-flow peristaltic pump pulse buffering; the pressure buffer area can be adjusted by adjusting the pressure of the internal sealed gas when the buffer is manufactured, so that volume production can be realized.

Description

Liquid road buffer-type sheath fluid steady flow system

Technical field

The present invention relates to one and be applied to flow cytometer Ye road buffer-type sheath fluid steady flow system.

Background technology

In small desk flow cytometer, in order to simplify design He Ye road, liquid road volume, adopt the peristaltic pump of low discharge to make fluid pipe produce negative pressure, sheath fluid is pumped in working fluid road.The introducing of peristaltic pump, has avoided the use of positive-pressure type vacuum pump, and the design of small desk flow cytometer and volume production are come true.

Peristaltic pump, just as be full of the flexible pipe of fluid with one of pinching of finger, moves forward along with pointing sliding tube inner fluid forward.Peristaltic pump is also that this principle has just replaced finger by roller.By the elasticity delivery hose to pump hocket extruding and discharge come pumping fluid.Just as with two finger pinching flexible pipes, along with the movement of finger, in pipe, form negative pressure, liquid flows thereupon, and peristaltic pump is exactly one section of pump line formation " pillow " shape fluid between two transfer roller.The volume of " pillow " depends on the internal diameter of pump line and the geometric properties of rotor.Flow depends on often the turn around product of these three parameters of number of " pillow " that produce of the rotating speed of pump head and the size of " pillow ", rotor.For sheath fluid, the size of " pillow " is generally constant.Compare with the pump that root diameter is identical, produce the pump of large " pillow " volume, its rotor carried fluid volume that often turns around is also larger, but the pulsation degree of generation is also larger.And producing the pump of less " pillow " volume, its rotor carried fluid volume that often turns around is also less; And little " pillow " forming rapidly, continuously makes flowing of fluid comparatively steady.

Just because of this principle of work of peristaltic pump, bring its intrinsic limitation: pump can produce a stream of pulses in the time of running, this stream of pulses makes sheath hydraulic coupling occur pulsation.Existing way is the multiple rotors of peristaltic pump band (3 of >) of selecting, and subtracts accordingly trochantinian diameter, makes to flow through in journey and produce less " pillow " volume as far as possible at pump, to weaken fluid pulse peak value as far as possible.

Sheath fluid pulsation brings following problem:

1) flow cytometer need to form the single cell suspension of laminar condition, cell is queued up single successively by the center of energy of flow chamber or central axis capillaceous and lasers excites spot.But sheath fluid pulsation can destroy this laminar condition, forms turbulent flow or turbulent flow, make cell suspension depart from Ye Liu center and laser focused in the center of flow chamber hot spot, even cause cell overlap.Affect detection resolution and sensitivity, directly cause the failure of an experiment.

2) use many rotors peristaltic pump can not eliminate intrinsic Ye road pulsation, simultaneously and can bring the unstable wear of increase, flow restriction and the peristaltic pump flexible pipe of cost.

Summary of the invention

The object of the invention is to overcome the deficiency that prior art exists, provide one to be applied to flow cytometer Ye road buffer-type sheath fluid steady flow system.

Object of the present invention is achieved through the following technical solutions:

Liquid road buffer-type sheath fluid steady flow system, feature is: comprise peristaltic pump and straight-through gasbag-type pulse buffer, straight-through gasbag-type pulse buffer comprises cylindrical housings and puts compressible Collapsible air bag in the inner, the output port of peristaltic pump is connected with the import of compressible Collapsible air bag by front liquid conduit, and the outlet of compressible Collapsible air bag is connected with streaming pond by rear liquid conduit; Between the outer wall of described compressible Collapsible air bag and the inwall of cylindrical housings, form the space of sealing and be filled with gas.

Further, above-mentioned Ye road buffer-type sheath fluid steady flow system, described front liquid conduit is connected with the import of compressible Collapsible air bag by pipe adapter.

Further, above-mentioned Ye road buffer-type sheath fluid steady flow system, described rear liquid conduit is connected with the outlet of compressible Collapsible air bag by pipe adapter.

The substantive distinguishing features of technical solution of the present invention and progressive being mainly reflected in:

The present invention, by straight-through gasbag-type pulse buffer is set, has greatly reduced fluid pulse, makes sheath fluid operation more steady; Do not need to increase length of pipe, for the peristaltic pump pulse buffer of low discharge, its design volume is small and exquisite, does not take up room; Can, by the time making impact damper, by adjusting the pressure of enclose inside gas, regulate between the buffer zone of pressure, can realize volume production; Solve the intrinsic pulsatile nature of existing peristaltic pump, do not changing under the prerequisite of peristaltic pump inner structure, lead directly to gasbag-type pulse buffer and eliminate the impact of Pulsating Flow by peripheral hardware, can use universal peristaltic pump, still reach pulseless pump stream effect.

Brief description of the drawings

Below in conjunction with accompanying drawing, technical solution of the present invention is described further:

Fig. 1: organigram of the present invention.

Embodiment

As shown in Figure 1, liquid road buffer-type sheath fluid steady flow system, compriseperistaltic pump 1 and straight-through gasbag-type pulse buffer 3, straight-through gasbag-type pulse buffer 3 comprises cylindrical housings 6 and puts compressibleCollapsible air bag 7 in the inner, the output port ofperistaltic pump 1 is connected with the import of compressibleCollapsible air bag 7 by front liquid conduit 2, and the outlet of compressibleCollapsible air bag 7 is connected withstreaming pond 10 by rear liquid conduit 9; Between the inwall of the outer wall of compressibleCollapsible air bag 7 and cylindrical housings 6, form the space 8 of sealing and be filled with gas.

Wherein, front liquid conduit 2 is connected with the import of compressibleCollapsible air bag 7 by pipe adapter 4, and has O-ring seal 5 to seal; Rear liquid conduit 9 is connected with the outlet of compressible Collapsible air bag by pipe adapter.

Peristaltic pump pumps the sheath fluid in sheath fluid bucket, because peristaltic pump can produce " pillow " shape fluid, cause producing in output pipe pulsing gluid, a straight-through gasbag-type pulse buffer 3 of peristaltic pump output terminal pipeline access, straight-through gasbag-type pulse buffer 3 inside are with compressibleCollapsible air bag 7, between compressibleCollapsible air bag 7 and cylindrical housings 6, be closed with the gas of certain pressure, utilize the compressibility of scalability and the outside confining gas of compressibleCollapsible air bag 7, the sheath hydraulic coupling by this inside air bag is cushioned.

In the time that the flow stream pressure of straight-through gasbag-type pulse buffer 3 left ends increases, compressibleCollapsible air bag 7 outwards expands under the effect of pressure, compress the gas between compressibleCollapsible air bag 7 and the wall of cylindrical housings 6, the volume of compressibleCollapsible air bag 7 increases, internal pressure diminishes, and flow stream pressure diminishes; In the time that the flow stream pressure of straight-through gasbag-type pulse buffer 3 left ends reduces, compressibleCollapsible air bag 7 inwardly shrinks under the effect of outside confining gas pressure, air bag smaller volume, and it is large that internal pressure becomes, and flow stream pressure is compensated.

By straight-through gasbag-type pulse buffer is set, greatly reduce fluid pulse, make sheath fluid operation more steady.Straight-through gasbag-type pulse buffer is set, does not need to increase length of pipe, for the peristaltic pump pulse buffer of low discharge, its design volume is small and exquisite, does not take up room.Can, by the time making impact damper, by adjusting the pressure of enclose inside gas, regulate between the buffer zone of pressure, can realize volume production.Be directed to the sheath fluid that flow cytometer uses, and peristaltic pump flow belongs to low discharge type, bag material can use conventional butyl, butyronitrile and fluororubber material.

Solve the intrinsic pulsatile nature of existing peristaltic pump, do not changing under the prerequisite of peristaltic pump inner structure, lead directly to gasbag-type pulse buffer and eliminate the impact of Pulsating Flow by peripheral hardware, can use universal peristaltic pump, still can reach pulseless pump stream effect.

It is emphasized that: be only preferred embodiment of the present invention above, not the present invention is done to any pro forma restriction, any simple modification, equivalent variations and modification that every foundation technical spirit of the present invention is done above embodiment, all still belong in the scope of technical solution of the present invention.

Claims (3)

1.Ye road buffer-type sheath fluid steady flow system, it is characterized in that: comprise peristaltic pump and straight-through gasbag-type pulse buffer, straight-through gasbag-type pulse buffer comprises cylindrical housings and puts compressible Collapsible air bag in the inner, the output port of peristaltic pump is connected with the import of compressible Collapsible air bag by front liquid conduit, and the outlet of compressible Collapsible air bag is connected with streaming pond by rear liquid conduit; Between the outer wall of described compressible Collapsible air bag and the inwall of cylindrical housings, form the space of sealing and be filled with gas.

2. liquid according to claim 1 road buffer-type sheath fluid steady flow system, is characterized in that: described front liquid conduit is connected with the import of compressible Collapsible air bag by pipe adapter.

3. liquid according to claim 1 road buffer-type sheath fluid steady flow system, is characterized in that: described rear liquid conduit is connected with the outlet of compressible Collapsible air bag by pipe adapter.

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