CN103734171A - Pesticide composition containing imidacloprid - Google Patents

Abstract

The invention discloses a pesticide composition containing imidacloprid, which contains an active component A, an active component B, and an active component C. The active component A is imidacloprid; the active component B is fludioxonil; and the active component C is difenoconazole. The composition of the invention can control diseases and insect attack of wheat, has obvious synergistic effect, extends the insecticidal and bacteriocidal spectra, has high activity on wheat sheath blight, root rot, piercing-sucking insects and aphids, decreases the drug dosage, reduces residual quantity, and mitigates environment pollution.

Description

A kind of composition pesticide containing Imidacloprid

Technical field

The present invention relates to a kind of mixed pesticide technology, specifically relate to a kind of by Imidacloprid, fludioxonil, composite pesticide-germicide seed treatment and the application thereof forming of Difenoconazole.

Background technology

In the real process of agricultural production, using chemical agent is the most effectively means of control plant pest, but a kind of chemical insecticide is prevented and treated single, if insecticide is mixed with bactericide, can a drug prevent and treat a plurality of targets, save labour cost, disposablely prevent and kill off disease, insect pest, so not only learn from other's strong points to offset one's weaknesses, bring into play various medicament strong points, reduce costs, and saving of work and time is obvious; Can increase economic efficiency like this, and reasonably by insecticide and bactericide compounded or to be mixed be one of the effective method the most addressing the above problem.

Wheat aphid belongs to Homoptera Aphidiadae, is commonly called as cockroach, aphid, aphid, is one of primary pest of wheat, can sting and inhale harm wheat, affects Wheat Photosynthesis effect and nutrient absorption, conduction.After wheat heading, concentrate on the harm of fringe portion, form blighted grain, thousand kernel weight is reduced and cause the underproduction.All there is generation in Ge Mai district in whole world.Main harm wheat class and other gramineous crops and weeds, nymph, adult Chang great Liang are clustered in blade, stem stalk, fringe portion absorption juice, by harm, are just yellow stigma, are streak afterwards, and withered, whole strain becomes till death withered.

Wheat sharp eyespot claims again wheat point eye spot disease (wheat sharpeyespot), almost spreads all over each wheat planting area, temperate zone, the world.All there is Infection and damage in various degree in China Yangtze river basin and Ge Mai district, the Plain of Huanghua Area, are the important diseases in these wheat district Wheat Production.Threat wheat producing region being caused in order to solve wheat sharp eyespot, Chinese scholars with regard to the cause of disease of this disease, cause harm and controlling way and method have been carried out a lot of research, and brought into play positive effect in pest controlling volume increase.

Banded sclerotial blight mainly occurs on wheat leaf sheath and stem stalk.After wheat jointing, symptom is obvious gradually.Their early stage produces yellowish-brown ellipse or fusiformis scab on the leaf sheath of earth's surface or near surface, after, sick portion expands gradually, darkens, and develops the stem of causing harm to the inside, and one, the two joint blackening of grave illness strain base portion are even rotten, normal Deaths.The scab on leaf sheath is moire shape decorative pattern to wheat growth to the later stage mid-term.Scab is random, when serious, surrounds full leaf sheath, makes leaf sheath and blade early withered.Large at field humidity, under the bad condition of air capacity of soils, between sick sheath and stem stalk or scab surface, often produces white mustiness thing.In the above, scattered khaki of initial stage or the little group of filemot mustiness, basidiospore is unicellular, ellipse or oblong, base portion is point slightly, colourless.The main distributed areas of wheat sharp eyespot: the Yangtze river basin, the Plain of Huanghua Area.Characteristics of incidence: the sclerotium of germ or mycelia are got over the summer or survive the winter on killed plant residue or in soil, become the Main Bacteria source of primary infection.New scab on killed plant can grow mycelia, stretches out host surface, to contiguous wheat strain, spreads and infects.Winter wheat sowing is too early, too close, and before the winter, wheat seeding is crossed prosperous or uses nitrogenous fertilizer too much, the wheatland of wheat seeding excessive growth, and suffer low temperature cold damage, the de-fertile or too many wheatland of pouring water spring, fall ill all heavier.Autumn and winter are warm, and rainy wet weather, used with invalid body and made muck and do not become thoroughly decomposed spring next year, and acid ground, was all conducive to morbidity.Disease resistance between wheat breed has notable difference.

Imidacloprid (Imidacloprid) belongs to nitro-methylene-type systemic insecticide, is the acting body of nicotinic acid nAChR ligands, disturbs pest movements nervous system that chemical signal is transmitted malfunctioning, without cross resistance problem.Be used for preventing and treating sucking pest and resistant strain thereof.Imidacloprid is chloro nicotine insecticide of new generation, has wide spectrum, efficient, low toxicity, low-residual, and insect is difficult for producing resistance, to features such as people, animal, plant and natural enemies securities, and have tag, stomach toxicity and the multiple drug effect of interior suction.After insect contact medicament, nervous centralis normal conduction is obstructed, and makes its paralysis dead.Quick-acting is good, within after medicine 1 day, has higher preventive effect, and residual life reached about 25 days.Drug effect and temperature are proportionate, and temperature is high, good disinsection effect.Be mainly used in preventing and treating sucking pest.

Fludioxonil (Fludioxoni) belongs to low toxic pesticide, by suppressing the relevant transfer of glucose phosphorylated, and the mycelial growth of Antifungi, finally cause germ dead.The mechanism of action is unique, with existing biocide agent without cross resistance.The wide-spectrum bactericide that belongs to non-internal-absorting.

Difenoconazole (difenoconazole) belongs to low toxicity bactericide, has protection, treatment and systemic activity, is sterol demethylation inhibitor, suppresses the biosynthesis of cell wall sterol, stops the growth of fungi.Fungicidal spectrum is wide, and leaf surface treatment or seed treatment can improve output and the guaranteed quality of crop.

Summary of the invention

The object of the present invention is to provide a kind of by Imidacloprid, fludioxonil, the composite composition pesticide of Difenoconazole.

Another object of the present invention is to prevent and treat banded sclerotial blight, aphid of wheat etc. and does not leave any the Harmful Residue that affects seed sprouting, seedling development, plant strain growth and product.

Implementation procedure of the present invention is as follows:

A composition pesticide containing Imidacloprid, by weight percentage, comprises following component:

In above-mentioned composition, the percentage by weight of Imidacloprid is: 5%-60%, the percentage by weight of fludioxonil is: 1%-10%, the percentage by weight of Difenoconazole is: 1%-10%.More preferably the percentage by weight of Imidacloprid is: 5%-50%, the percentage by weight of fludioxonil is: 1%-5%, the percentage by weight of Difenoconazole is: 1%-5%.

Above-mentioned composition can be processed as suspension seed-coating agent.

The advantage of suspension seed-coating agent: slow release is good, biologically active is high, safety.Good effect is: after seed dressing, medicament can slowly discharge, and aphid and banded sclerotial blight during control wheat growth, reduce medicament afterwards and spray, and reduces man power and material, and wheat increase yield and increasing peasant income are had to great benefit.

Following specific embodiment, in order to the present invention to be described, still the invention is not restricted to following formula, and in formula, each component is all bought from other producers, and concrete formula is as follows:

Example one, 52% Imidacloprid fludioxonil difenoconazole suspending seed-dressing agent

Example two, 55% Imidacloprid fludioxonil difenoconazole suspending seed-dressing agent

Example three, 50% Imidacloprid fludioxonil difenoconazole suspending seed-dressing agent

Example four, 25% Imidacloprid fludioxonil difenoconazole suspending seed-dressing agent

Example five, 32% Imidacloprid fludioxonil difenoconazole suspending seed-dressing agent

Embodiment:

One, Imidacloprid, the indoor biometrics test of fludioxonil proportionings different from Difenoconazole to wheat aphid

1.1 reagent agent

The former medicine of 97% Imidacloprid, the former medicine of 95% fludioxonil, 97% difenoconazole technical material, is my company and offers Tianjin Plant Protection Institute

1.2 for examination worm source

(Sitobion avenae (Fabri.) picks up from Wuqing District, academy of agricultural sciences, Tianjin science and technology innovation bases experimental field of wheat to English grain aphid

1.3 compounding method for medicament

Get the former medicine 1.0g of 97% Imidacloprid, with the DMF of 5ml, dissolve, then add and contain 200 times of mother liquors that 0.1% the Tween 80 aqueous solution is configured to 200ml, adopt double dilution method, 5 concentration gradient dilutions of corresponding configuration are to be measured.Get the former medicine 1.0g of 95% fludioxonil, with the DMF of 5ml, dissolve, then add and contain 200 times of mother liquors that 0.1% the Tween 80 aqueous solution is configured to 200ml, adopt double dilution method, 5 concentration gradient dilutions of corresponding configuration are to be measured.Get 97% difenoconazole technical material 1.0g, with the DMF of 5ml, dissolve, then add and contain 200 times of mother liquors that 0.1% the Tween 80 aqueous solution is configured to 200ml, adopt double dilution method, 5 concentration gradient dilutions of corresponding configuration are to be measured.Imidacloprid, fludioxonil, Difenoconazole take respectively the former medicine of corresponding quality by 70+1+1,60+1+1,50+1+1,40+1+1, five proportionings of 30+1+1 respectively, first with DMF, dissolve, add again and contain appropriate 0.1% the Tween 80 aqueous solution and be configured to 200 times of mother liquors, adopt dilution method double, 5 concentration gradient dilutions of corresponding configuration are to be measured.

1.4 chemicals treatment

Select tape root without worm, healthy and strong wheat plant, root is carefully cleaned, dried, plant root is inserted and is equipped with in the beaker of dilute liquid medicine, and rim of a cup supports with doubling plastic plate with holes, is put in climatic cabinate and cultivates, after 24 hours, plant is taken out from liquid, cut the part that plant neck and fringe portion do not touch medicament and be put in culture dish, moisturizing, access examination 15 left and right of worm, every processing repeats 4 times.And to establish 10% imidacloprid wettable powder 50mg/L dilution be control treatment, separately establish not processing containing medicament as blank.

1.5 investigation

Process the death condition of latter 24 hours investigation aphids, the total examination of record borer population, death toll and insect population decline rate.

Dead criterion: touch polypide, feeler, the stiff person of four limbs and be considered as death.

1.6 data statistics and analysis

Computer programming calculates the LC of each medicament₅₀, LC₉₀, b value, standard error and 95% confidence limit thereof, and ask and calculate co-toxicity coefficient CTC.

1.7 toxicity test results

Table 1 Imidacloprid, fludioxonil, the indoor measurement result of Difenoconazole to wheat aphid

As can be seen from the table, in being mixed of different proportion (70+1+1,60+1+1,50+1+1,40+1+1,30+1+1), its co-toxicity coefficient is summation action except the co-toxicity coefficient of (30+1+1) is less than 120, all the other are all greater than 120 for synergistic effect, wherein the synergistic effect of 70:1:1 is the most obvious, and co-toxicity coefficient is 259.89.

Two, Imidacloprid, the formulation screening test of fludioxonil proportionings different from Difenoconazole to wheat aphid

1.1 reagent agent

The former medicine of 97% Imidacloprid, the former medicine of 95% fludioxonil, 97% difenoconazole technical material, is my company and offers Tianjin Plant Protection Institute

1.2 for examination worm source

Rhizoctonia cerealis (Rhizoctonia solani), picks up from academy of agricultural sciences, Tianjin Plant Protection Institute and plants ward preservation

1.3 compounding method for medicament

First carry out preliminary experiment, the liquor strength when tentatively finding out bacteriostasis rate and being 10～80%, sets concentration gradient accordingly.With ten thousand/balance, take respectively medicament.The former medicinal a small amount of acetone solution of Imidacloprid, be diluted to the mother liquor of 10000 mcg/ml containing 0.05% Tween 80 sterile water, is once diluted to variable concentrations gradient; Fludioxonil, Difenoconazole dissolve (the final content < 0.5% of solvent) with a small amount of dimethyl formamide, then with the sterile water containing 0.05% Tween 80, are diluted to the mother liquor of 10000 mcg/ml.Be diluted to successively more different concentration gradients.Imidacloprid, fludioxonil, Difenoconazole mixture are drawn corresponding mother liquor by different proportionings, then are diluted to respectively different concentration gradients.

1.4 chemicals treatment

Drawing 4ml liquid fully mixes in 50ml sterilizing triangular flask with the 36mlPDA medium melting in advance, be made into the pastille medium (in Table 1) of series concentration, after fully shaking up, pour sterilizing plate into, the pastille of making respective concentration is dull and stereotyped, contains the blank that is treated to of 0.05% Tween 80 not add liquid.After culture medium solidifying, access the rhizoctonia cerealis sheet that diameter 5mm has activated, in triplicate, every ware 2 ferfas sheets, are placed in 25 ℃ of insulating boxs by germ in every processing.

1.5 control times and number of times

After cultivation 4d, the mycelia of blank has been covered with full ware 2/3, uses kind of calliper colony radius, and each bacterium colony right-angled intersection method vertical survey respectively once.

1.6 data statistics and analysis

According to survey data, calculate the mycelial growth inhibition rate that each is processed.Computing formula is as follows:

Colony radius value added=colony radius-bacterium sheet radius

Mycelial growth inhibition rate %=(blank colony radius value added-chemicals treatment colony radius value added) * 100/ blank colony radius value added

According to the concentration value of each medicament and corresponding mycelial growth inhibition rate probability value, do regression analysis, calculate the EC of each medicament₅₀, EC₉₀value and 95% confidence limit thereof.

The EC of Imidacloprid, fludioxonil, Difenoconazole and Imidacloprid fludioxonil Difenoconazole mixture₅₀measured value is drawn by regression equation, is expressed as EC_50(pyrrole), EC_{50(coughs up)}, EC_50(benzene), EC_50(ob).The theoretical value computing formula of Imidacloprid fludioxonil Difenoconazole mixture is: EC_50(th)=(a+b+c)/(a/EC_50(pyrrole)+ b/EC_{50(coughs up)}+ c/EC_50(benzene)).Wherein a, b, c represent Imidacloprid, fludioxonil and Difenoconazole shared ratio in mixture.

Degree of interaction (synergism value) SR=EC of Imidacloprid, fludioxonil and Difenoconazole mixture_50(th)/ EC_50(ob).If SR < 0.5 is antagonism reaction; SR=0.5～1.5 are additions; SR > 1.5 is synergistic effects.

1.7 the result EC of toxicity test₅₀

As can be seen from the table, in being mixed of different proportion (50:1:5,50:1:3,50:1:1,50:3:1,50:5:1), its co-toxicity coefficient is except the synergism value of (50:1:5) is 0.5～1.5th, summation action, all the other are all greater than 1.5 for synergistic effect, wherein the synergistic effect of 50:1:1 is the most obvious, and synergism value is 5.56.

Claims (5)

1. an insecticidal bactericide that contains Imidacloprid, fludioxonil and Difenoconazole, is characterized in that: the percentage by weight of described Imidacloprid is: 5%～60%, and the percentage by weight of fludioxonil is 1%～10%, the percentage by weight of Difenoconazole is: 1%～10%.

2. insecticidal bactericide according to claim 1, is characterized in that: the preferred weight percent of Imidacloprid is: 5%～50%, the preferred weight percent of fludioxonil is: 1%～5%, Difenoconazole preferred weight percent be: 1%～5%.

3. according to claim 1 containing the insecticidal bactericide of Imidacloprid, fludioxonil and Difenoconazole, it is characterized in that: make suspension seed-coating agent.

According to claim 1 containing the insecticidal bactericide of Imidacloprid, fludioxonil and Difenoconazole in the application aspect control crop pests, germ.

5. containing the insecticidal bactericide of Imidacloprid, fludioxonil and Difenoconazole, mainly prevent and treat wheat class sucking pest aphid, wheat sharp eyespot according to claim 4.