CN103424363A - Non-rotary optical rotation solution gauge and method for measuring specific rotation of optical rotation solution through gauge - Google Patents

Abstract

Translated fromChinese

非旋转式旋光溶液测量仪及采用该测量仪测量旋光溶液旋光率的方法，涉及一种旋光溶液测量方法。本发明解决了现有测量旋光物质的仪器在测量溶液旋光率时需要不断旋转检偏器确定旋光溶液振动面的转动角度的问题。本发明利用光源元件发射出的光束入射至起偏器经起偏器起偏后的线偏振光入射至装有旋光溶液的试管，经装有旋光溶液的试管后的线偏振光入射至显偏器，经显偏器显偏后的线偏振光入射至图像采集装置感光面上，图像采集装置将采集到的图像信息发送至PC机，PC机对待测溶液后获得的光束图像信息中光束的偏振方向与未经待测溶液的光束图像信息中光束的偏振方向作差，获得待测溶液旋光度θ，最后获得待测溶液旋光率。本发明适用于测量旋光溶液。

The invention discloses a non-rotating optical rotation solution measuring instrument and a method for measuring the optical rotation rate of an optical rotation solution by using the measuring instrument, relating to a method for measuring an optical rotation solution. The invention solves the problem that the existing instruments for measuring optically active substances need to continuously rotate the polarizer to determine the rotation angle of the vibration plane of the optically active solution when measuring the optically active rate of the solution. In the present invention, the light beam emitted by the light source element is incident on the polarizer, the linearly polarized light polarized by the polarizer is incident on the test tube containing the optical rotation solution, and the linearly polarized light after passing through the test tube containing the optical rotation solution is incident on the polarizer The linearly polarized light after being polarized by the polarizer is incident on the photosensitive surface of the image acquisition device, and the image acquisition device sends the collected image information to the PC. The polarization direction is different from the polarization direction of the beam in the beam image information of the untested solution to obtain the optical rotation θ of the solution to be tested, and finally the optical rotation of the solution to be tested is obtained. The invention is suitable for measuring optically active solutions.

Description

Translated fromChinese

非旋转式旋光溶液测量仪及采用该测量仪测量旋光溶液旋光率的方法Non-rotating optical rotation solution measuring instrument and method for measuring optical rotation of optical solution using the measuring instrument

技术领域technical field

本发明涉及一种旋光溶液测量方法。The invention relates to a method for measuring an optically active solution.

背景技术Background technique

线偏振光通过某些物质的溶液后，偏振光的振动面将以光的传播方向为轴旋转一定的角度，这种现象称为旋光现象。旋转的角度称为该物质的旋光度。通常用旋光仪来测量物质的旋光度。After the linearly polarized light passes through the solution of certain substances, the vibration plane of the polarized light will rotate at a certain angle with the direction of light propagation as the axis. This phenomenon is called optical rotation. The angle of rotation is called the optical rotation of the substance. A polarimeter is usually used to measure the optical rotation of a substance.

旋光性物质有右旋和左旋之分。当面对光射来方向观察，如果振动面按顺时针方向旋转，则称右旋物质；如果振动面向逆时针方向旋转，称左旋物质。Optically active substances can be divided into right-handed and left-handed. When viewed from the direction of light, if the vibrating surface rotates clockwise, it is called a right-handed substance; if the vibrating surface rotates counterclockwise, it is called a left-handed substance.

现有测量旋光物质测量在光具座上先将半导体激光器发出的激光束与起偏器、光功率计探头调节成等高同轴。调节起偏器转盘，使输出偏振光最强（半导体激光器发出的是部分偏振光），再将检偏器放在光具座的滑块上，使检偏器与起偏器等高同轴（检偏器与起偏器平行）。调节检偏器转盘使从检偏器输出光强为零(一般调不到零，只能调到最小)，此时检偏器的透光轴与起偏器的透光轴相互垂直，继续调节检器转盘，使从检偏器输出光强再次为零或者最小，分别读出这两次光强为零时检偏器转盘的读数，应该相差180°。In the existing measurement of optically active substances, on the optical bench, the laser beam emitted by the semiconductor laser, the polarizer, and the probe of the optical power meter are adjusted to be of equal height and coaxial. Adjust the polarizer turntable to make the output polarized light the strongest (semiconductor laser emits partially polarized light), and then place the analyzer on the slider of the optical bench so that the analyzer and the polarizer are coaxial at the same height (the analyzer is parallel to the polarizer). Adjust the polarizer turntable so that the output light intensity from the polarizer is zero (generally it cannot be adjusted to zero, but can only be adjusted to the minimum). At this time, the light transmission axis of the polarizer and the light transmission axis of the polarizer are perpendicular to each other. Continue Adjust the detector turntable so that the output light intensity from the polarizer is zero or minimum again, and read the readings of the analyzer turntable when the light intensity is zero for these two times, and the difference should be 180°.

将样品管（内有旋光液体）放于支架上，用白纸片观察偏振光入射至样品管的光点和从样品管出射光点形状是否相同，以检验玻璃是否与激光束等高同轴。调节检偏器转盘，观察旋光液体的旋光特性，是右旋溶液还是左旋溶液。Put the sample tube (with optically active liquid inside) on the bracket, and use a white paper to observe whether the shape of the light spot of the polarized light entering the sample tube is the same as that of the light spot exiting the sample tube, so as to check whether the glass is coaxial with the laser beam. . Adjust the rotating disk of the polarizer to observe the optical rotation characteristics of the optical liquid, whether it is a right-handed solution or a left-handed solution.

在测量时，比较困难的是，如何确定旋光仪的零点校正值、旋光物质的旋光方向和求算比旋光度。由于偏振光的振动面是随着光在旋光物质中向前进行而逐渐旋转的，传统的测量旋光物质的仪器需要不断旋转检偏器以确定旋光溶液振动面的转动角度。When measuring, it is more difficult to determine the zero point correction value of the polarimeter, the direction of rotation of the optically active substance, and the calculation of the specific rotation. Since the vibration plane of polarized light gradually rotates as the light moves forward in the optically active substance, traditional instruments for measuring optically active substances need to continuously rotate the analyzer to determine the rotation angle of the vibration plane of the optically active solution.

发明内容Contents of the invention

本发明为了解决现有测量旋光物质的仪器在测量溶液旋光率时需要不断旋转检偏器确定旋光溶液振动面的转动角度的问题，提出了一种非旋转式旋光溶液测量仪及采用该测量仪测量旋光溶液的方法。In order to solve the problem that the existing instruments for measuring optically active substances need to continuously rotate the polarizer to determine the rotation angle of the vibration surface of the optically active solution when measuring the optically active rate of the solution, the present invention proposes a non-rotating optically active solution measuring instrument and adopts the measuring instrument Method for measuring optically active solutions.

本发明所述非旋转式旋光溶液测量仪，该旋光仪包括光源元件、起偏器、试管、试管架、显偏器、图像采集装置和PC机；The non-rotating polarimetric solution measuring instrument of the present invention includes a light source element, a polarizer, a test tube, a test tube rack, a polarizer, an image acquisition device, and a PC;

光源元件发射出的光束入射至起偏器经起偏器起偏后的线偏振光入射至装有旋光溶液的试管，经装有旋光溶液的试管后的线偏振光入射至显偏器，经显偏器显偏后的线偏振光入射至图像采集装置的感光面上，图像采集装置将采集到的图像信息发送至PC机，试管安装在试管架上，试管用于盛装待测溶液，且试管的中心与经过起偏器起偏后的偏振光束的光轴在同一条直线上。The light beam emitted by the light source element enters the polarizer, and the linearly polarized light after being polarized by the polarizer enters the test tube containing the optically active solution, and the linearly polarized light after passing through the test tube containing the optically active solution enters the polarizer, and passes through the polarizer. The linearly polarized light after polarized by the polarizer is incident on the photosensitive surface of the image acquisition device, the image acquisition device sends the collected image information to the PC, the test tube is installed on the test tube rack, and the test tube is used to contain the solution to be tested, and The center of the test tube is on the same straight line as the optical axis of the polarized beam polarized by the polarizer.

采用上述装置测量旋光溶液的方法，该方法的具体步骤为：Adopt the method for measuring optically active solution of above-mentioned device, the concrete steps of this method are:

步骤一、打开光源元件，光源光束经起偏器启偏后入射至显偏器，经显偏器后的光束入射到图像采集装置的感光面上，Step 1. Turn on the light source element, the light beam of the light source is incident on the polarizer after being polarized by the polarizer, and the light beam after passing through the polarizer is incident on the photosensitive surface of the image acquisition device,

步骤二、图像采集装置对入射光束进行图像信息采集，并将采集到的光束图像信息传递给PC机，PC机提取获得光束图像信息中光束的偏振方向。Step 2: The image acquisition device collects the image information of the incident light beam, and transmits the collected light beam image information to the PC, and the PC extracts and obtains the polarization direction of the light beam in the light beam image information.

步骤三、将待测旋光溶液装入试管，并将试管安装在试管架上，光源光束经起偏器启偏后入射至盛有待测旋光溶液的试管；Step 3, put the optical rotation solution to be tested into the test tube, and install the test tube on the test tube rack, and the light beam is incident on the test tube containing the optical rotation solution to be tested after being polarized by the polarizer;

步骤四、偏振光经过盛有待测溶液的试管后入射至显偏器，经显偏器后的偏振光入射至图像采集装置的感光面上，图像采集装置对入射光束进行图像信息采集；Step 4, the polarized light is incident on the polarizer after passing through the test tube containing the solution to be tested, and the polarized light after passing through the polarizer is incident on the photosensitive surface of the image acquisition device, and the image acquisition device collects image information of the incident light beam;

步骤五、图像采集装置将采集到的光束图像信息传递给PC机，PC机提取获得光束图像信息中光束的偏振方向；Step 5, the image acquisition device transmits the collected beam image information to the PC, and the PC extracts and obtains the polarization direction of the beam in the beam image information;

步骤六、PC机对步骤五获得的光束图像信息中光束的偏振方向与步骤二获得的光束图像信息中光束的偏振方向作差，获得待测溶液的旋光度θ；Step 6, the PC makes a difference between the polarization direction of the beam in the beam image information obtained instep 5 and the polarization direction of the beam in the beam image information obtained instep 2, to obtain the optical rotation θ of the solution to be measured;

步骤七、利用公式Step 7. Use the formula

${<span><span>[</span>[</span>\mathrm{<span><span>\&alpha;</span>\&alpha;</span>}<span><span>]</span>]</span>}_{\mathrm{<span><span>\&lambda;</span>\&lambda;</span>}}^{\mathrm{<span><span>t</span>t</span>}}<span><span>=</span>=</span>\frac{\mathrm{<span><span>\&theta;</span>\&theta;</span>}}{\mathrm{<span><span>l</span>l</span>}<span><span>\&CenterDot;</span>\&CenterDot;</span>\mathrm{<span><span>C</span>C</span>}}$

计算获得待测溶液的旋光率；Calculate the optical rotation of the solution to be measured;

式中，

右上角的t表示实验时温度，单位：℃，λ是旋光仪采用的单色光源的波长，单位：nm，θ为测得的旋光度，l为样品管的长度，单位：dm，C为溶液浓度，单位：g/100mL。In the formula,

The t in the upper right corner indicates the temperature during the experiment, unit: ℃, λ is the wavelength of the monochromatic light source used by the polarimeter, unit: nm, θ is the measured optical rotation, l is the length of the sample tube, unit: dm, C is Solution concentration, unit: g/100mL.

本发明采用显偏器替换原有的检偏器，实现了线偏振光的可视化和实时化检测，在溶液检测的过程中不需要不断旋转检偏器便可以检测出线偏振光经过旋光溶液后振动面的旋转角度，并可以将线偏振光的偏振方向在PC机上实时显示出来，从而实现了对偏振态动态变化的实时观察。The present invention replaces the original polarizer with a polarizer to realize the visualization and real-time detection of linearly polarized light. In the process of solution detection, it is not necessary to continuously rotate the polarizer to detect that the linearly polarized light vibrates after passing through the optical solution. The rotation angle of the plane, and the polarization direction of the linearly polarized light can be displayed on the PC in real time, so as to realize the real-time observation of the dynamic change of the polarization state.

附图说明Description of drawings

图1为本发明所述装置的结构示意图。Fig. 1 is a schematic structural view of the device of the present invention.

具体实施方式Detailed ways

具体实施方式一、结合图1说明本实施方式，本实施方式所述非旋转式旋光溶液测量仪，该旋光仪包括光源元件1、起偏器2、试管3、试管架4、显偏器5、图像采集装置6和PC机7；Specific Embodiments 1. This embodiment is described in conjunction with FIG. 1. The non-rotating polarimetric solution measuring instrument described in this embodiment includes alight source element 1, apolarizer 2, atest tube 3, atest tube rack 4, and apolarizer 5. ,image acquisition device 6 and PC 7;

光源元件1发射出的光束入射至起偏器2经起偏器2起偏后的线偏振光入射至装有旋光溶液的试管3，经装有旋光溶液的试管3后的线偏振光入射至显偏器4，经显偏器4显偏后的偏振光入射至图像采集装置6的感光面上，图像采集装置6将采集到的图像信息发送至PC机7，试管3安装在试管架4上，试管3用于盛装待测溶液，且试管3的中心与经过起偏器2起偏后的偏振光束的光轴在同一条直线上。The light beam emitted by thelight source element 1 is incident on thepolarizer 2, and the linearly polarized light after being polarized by thepolarizer 2 is incident on thetest tube 3 containing the optically active solution, and the linearly polarized light after passing through thetest tube 3 containing the optically active solution is incident on the Thepolarizer 4, the polarized light developed by thepolarizer 4 is incident on the photosensitive surface of theimage acquisition device 6, and theimage acquisition device 6 sends the collected image information to the PC 7, and thetest tube 3 is installed on thetest tube rack 4 Above, thetest tube 3 is used to contain the solution to be tested, and the center of thetest tube 3 is on the same straight line as the optical axis of the polarized beam polarized by thepolarizer 2 .

将显偏器替换原有的检偏器。实现线偏振光的可视化和实时化检测，不需要不断地旋转便可以检测出入射线偏振光的角度以及线偏振光经过旋光溶液后振动面的转动角度，减少了机械转动产生的误差提高了检测精度，并且可以将线偏振光的偏振方向在PC机上实时显示。同时，可以很方便的判断出样品管中的物质是左旋物质或者右旋物质。Replace the original polarizer with the polarizer. Realize the visualization and real-time detection of linearly polarized light. It can detect the angle of the incident ray polarized light and the rotation angle of the vibration plane after the linearly polarized light passes through the optical solution without continuous rotation, which reduces the error caused by mechanical rotation and improves the detection accuracy. , and the polarization direction of linearly polarized light can be displayed on the PC in real time. At the same time, it can be easily judged whether the substance in the sample tube is a left-handed substance or a right-handed substance.

具体实施方式二、本实施方式是对具体实施方式一所述的非旋转式旋光溶液测量仪进一步说明，光源元件1采用激光器或LED光源实现。采用激光器或LED光源作为入射光源，照射到起偏器上，经过显偏器，在PC机上得到入射光线偏振光的方向。Embodiment 2. This embodiment is a further description of the non-rotating polarimetric solution measuring instrument described inEmbodiment 1. Thelight source element 1 is realized by a laser or an LED light source. Use a laser or LED light source as the incident light source, irradiate on the polarizer, pass through the polarizer, and obtain the polarized direction of the incident light on the PC.

具体实施方式三、本实施方式是对具体实施方式一或具体实施方式二所述的非旋转式旋光溶液测量仪进一步说明，图像采集装置6采用CCD摄像机实现。Embodiment 3. This embodiment is a further description of the non-rotating polarimetric solution measuring instrument described inEmbodiment 1 orEmbodiment 2. Theimage acquisition device 6 is realized by a CCD camera.

采用CCD摄像机对经过4的线偏振光进行实时显示图像，并且通过PC机上的软件处理便可以精确得到线偏振光的出射方向。The CCD camera is used to display the image of the linearly polarized light after 4 in real time, and the outgoing direction of the linearly polarized light can be accurately obtained through the software processing on the PC.

具体实施方式四、本实施方式是采用具体实施方式一所述的非旋转式旋光溶液测量仪测量旋光溶液旋光率的方法，该方法的具体步骤为：Embodiment 4. This embodiment is a method for measuring the optical rotation of an optically active solution using the non-rotating optical solution measuring instrument described inEmbodiment 1. The specific steps of the method are:

步骤一、打开光源元件1，光源光束经起偏器2启偏后入射至显偏器5，经显偏器5后的光束入射到图像采集装置6的感光面上；Step 1: Turn on thelight source element 1, the light beam of the light source is incident on thepolarizer 5 after being polarized by thepolarizer 2, and the light beam after passing through thepolarizer 5 is incident on the photosensitive surface of theimage acquisition device 6;

步骤二、图像采集装置6对入射光束进行图像信息采集，并将采集到的光束图像信息传递给PC机7，PC机7提取获得光束图像信息中光束的偏振方向；Step 2, theimage acquisition device 6 collects the image information of the incident beam, and transmits the collected beam image information to thePC 7, and thePC 7 extracts and obtains the polarization direction of the beam in the beam image information;

步骤三、将待测溶液装入试管3，并将试管3安装在试管架4上，光源光束经起偏器2启偏后入射至盛有待测溶液的试管；Step 3, the solution to be tested is loaded into thetest tube 3, and thetest tube 3 is installed on thetest tube rack 4, and the light beam is incident on the test tube containing the solution to be tested after being polarized by thepolarizer 2;

步骤四、偏振光经过盛有待测溶液的试管3后入射至显偏器5，经显偏器5后的偏振光入射至图像采集装置6的感光面上，图像采集装置6对入射光束进行图像信息采集；Step 4, the polarized light is incident on thepolarizer 5 after passing through thetest tube 3 containing the solution to be tested, and the polarized light after passing through thepolarizer 5 is incident on the photosensitive surface of theimage acquisition device 6, and theimage acquisition device 6 performs a process on the incident light beam. Image information collection;

步骤五、图像采集装置6将采集到的光束图像信息传递给PC机7，PC机7提取获得光束图像信息中光束的偏振方向；Step 5, theimage acquisition device 6 transmits the collected beam image information to thePC 7, and thePC 7 extracts and obtains the polarization direction of the beam in the beam image information;

步骤六、PC机7对步骤二获得的光束图像信息中光束的偏振方向与步骤五获得的光束图像信息中光束的偏振方向作差，获得待测溶液的旋光度θ；Step 6, thePC 7 makes a difference between the polarization direction of the beam in the beam image information obtained instep 2 and the polarization direction of the beam in the beam image information obtained instep 5, to obtain the optical rotation θ of the solution to be measured;

步骤七、利用公式Step 7. Use the formula

${<span><span>[</span>[</span>\mathrm{<span><span>\&alpha;</span>\&alpha;</span>}<span><span>]</span>]</span>}_{\mathrm{<span><span>\&lambda;</span>\&lambda;</span>}}^{\mathrm{<span><span>t</span>t</span>}}<span><span>=</span>=</span>\frac{\mathrm{<span><span>\&theta;</span>\&theta;</span>}}{\mathrm{<span><span>l</span>l</span>}<span><span>\&CenterDot;</span>\&CenterDot;</span>\mathrm{<span><span>C</span>C</span>}}$

计算获得待测溶液的旋光率，Calculate the optical rotation of the solution to be tested,

式中，

右上角的t表示实验时温度，单位：℃，λ是旋光仪采用的单色光源的波长，单位：nm，θ为测得的旋光度，l为样品管的长度，单位：dm，C为溶液浓度，单位：g/100mL。In the formula,

The t in the upper right corner indicates the temperature during the experiment, unit: ℃, λ is the wavelength of the monochromatic light source used by the polarimeter, unit: nm, θ is the measured optical rotation, l is the length of the sample tube, unit: dm, C is Solution concentration, unit: g/100mL.

本实施方式在非旋转式旋光仪中用显偏器代替原有的检偏器。在测试时，当线偏振光入射时，无需调整显偏器，在输出端通过CCD成像便可以在PC机上实时的观测到输出图像，通过图像处理便可以得到线偏振光的偏振方向。同时，可以很方便的判断出样品管中的旋光物质是左旋物质或者右旋物质。In this embodiment, the original polarizer is replaced by a polarizer in a non-rotating polarimeter. During the test, when the linearly polarized light is incident, there is no need to adjust the polarizer, and the output image can be observed on the PC in real time through CCD imaging at the output end, and the polarization direction of the linearly polarized light can be obtained through image processing. At the same time, it can be easily judged whether the optically active substance in the sample tube is a levorotatory substance or a dextrorotatory substance.

具体实施方式五、本实施方式是对具体实施方式四所述的非旋转式旋光溶液测量仪测量旋光溶液旋光率的方法进一步说明，步骤七中λ为632.8nm。Embodiment 5. This embodiment is a further description of the method for measuring the optical rotation of an optically active solution by the non-rotating optical solution measuring instrument described inEmbodiment 4. Instep 7, λ is 632.8 nm.

本发明将显偏器替换原有的检偏器。实现线偏振光的可视化和实时化检测，不需要不断地旋转检偏器便可以检测出入射线偏振光的角度以及线偏振光的经过旋光溶液后振动面的转动角度，减少了机械转动产生的误差提高了检测精度，并且可以将线偏振光的偏振方向在PC机上实时显示，从而实现了对偏振态动态变化的实时观察。同时，可以很方便的判断出样品管中的物质是左旋物质或者右旋物质。The invention replaces the original polarizer with a polarizer. Realize the visualization and real-time detection of linearly polarized light, without constantly rotating the analyzer to detect the angle of incident ray polarized light and the rotation angle of the vibration plane of linearly polarized light after passing through the optical solution, reducing the error caused by mechanical rotation The detection accuracy is improved, and the polarization direction of the linearly polarized light can be displayed on the PC in real time, thereby realizing the real-time observation of the dynamic change of the polarization state. At the same time, it can be easily judged whether the substance in the sample tube is a left-handed substance or a right-handed substance.

Claims (5)

Translated fromChinese

1.非旋转式旋光溶液测量仪，其特征在于，该旋光仪包括光源元件（1）、起偏器（2）、试管（3）、试管架（4）、显偏器（5）、图像采集装置（6）和PC机（7）；1. Non-rotating polarimetric solution measuring instrument, characterized in that the polarimeter includes a light source element (1), a polarizer (2), a test tube (3), a test tube rack (4), a polarizer (5), an image Acquisition device (6) and PC (7);光源元件（1）发射出的光束入射至起偏器（2）经起偏器（2）起偏后的线偏振光入射至装有旋光溶液的试管（3），经装有旋光溶液的试管（3）后的线偏振光入射至显偏器（4），经显偏器（4）显偏后的线偏振光入射至图像采集装置（6）的感光面上，图像采集装置（6）将采集到的图像信息发送至PC机（7），试管（3）安装在试管架（4）上，试管（3）用于盛装待测溶液，且试管（3）的中心与经过起偏器（2）起偏后的偏振光束的光轴在同一条直线上。The light beam emitted by the light source element (1) is incident on the polarizer (2), and the linearly polarized light after being polarized by the polarizer (2) is incident on the test tube (3) containing the optical solution, and passes through the test tube containing the optical solution. The linearly polarized light after (3) is incident on the polarizer (4), and the linearly polarized light after being polarized by the polarizer (4) is incident on the photosensitive surface of the image acquisition device (6), and the image acquisition device (6) The collected image information is sent to the PC (7), the test tube (3) is installed on the test tube rack (4), the test tube (3) is used to contain the solution to be tested, and the center of the test tube (3) passes through the polarizer (2) The optical axes of the polarized beams are on the same straight line.2.根据权利要求1所述的非旋转式旋光溶液测量仪法，其特征在于，光源元件（1）采用半导体激光器实现。2. The non-rotating polarimetric solution measurement method according to claim 1, characterized in that the light source element (1) is realized by a semiconductor laser.3.根据权利要求1或2所述的非旋转式旋光溶液测试仪法，其特征在于，图像采集装置（6）采用CCD摄像机实现。3. The method according to claim 1 or 2, wherein the image acquisition device (6) is realized by a CCD camera.4.采用权利要求1所述的非旋转式旋光溶液测量仪测量旋光溶液旋光率的方法，其特征在于，该方法的具体步骤为：4. adopt the method for non-rotating optical solution measuring instrument of claim 1 to measure optical rotation rate of optical solution, it is characterized in that, the concrete steps of this method are:步骤一、打开光源元件（1），光源光束经起偏器（2）启偏后入射至显偏器（5），经显偏器（5）后的光束入射到图像采集装置（6）的感光面上，Step 1. Turn on the light source element (1), the light beam is incident on the polarizer (5) after being polarized by the polarizer (2), and the beam after passing through the polarizer (5) is incident on the image acquisition device (6) photosensitive surface,步骤二、图像采集装置（6）对入射光束进行图像信息采集，并将采集到的光束图像信息传递给PC机（7），PC机（7）提取获得光束图像信息中光束的偏振方向。Step 2: The image acquisition device (6) collects the image information of the incident light beam, and transmits the collected light beam image information to the PC (7), and the PC (7) extracts the polarization direction of the light beam in the obtained light beam image information.步骤三、将待测旋光溶液装入试管（3），并将试管（3）安装在试管架（4）上，光源光束经起偏器（2）启偏后入射至盛有待测旋光溶液的试管；Step 3. Put the optical rotation solution to be tested into the test tube (3), and install the test tube (3) on the test tube rack (4). the test tube;步骤四、偏振光经过盛有待测溶液的试管（3）后入射至显偏器（5），经显偏器（5）后的偏振光入射至图像采集装置（6）的感光面上，图像采集装置（6）对入射光束进行图像信息采集；Step 4: The polarized light is incident on the polarizer (5) after passing through the test tube (3) containing the solution to be tested, and the polarized light after passing through the polarizer (5) is incident on the photosensitive surface of the image acquisition device (6), The image acquisition device (6) collects image information of the incident light beam;步骤五、图像采集装置（6）将采集到的光束图像信息传递给PC机（7），PC机（7）提取获得光束图像信息中光束的偏振方向；Step 5, the image acquisition device (6) transmits the collected beam image information to the PC (7), and the PC (7) extracts the polarization direction of the beam in the beam image information;步骤六、PC机（7）对步骤五获得的光束图像信息中光束的偏振方向与步骤二获得的光束图像信息中光束的偏振方向作差，获得待测溶液的旋光度θ；Step 6. The PC (7) makes a difference between the polarization direction of the beam in the beam image information obtained in step 5 and the polarization direction of the beam in the beam image information obtained in step 2 to obtain the optical rotation θ of the solution to be measured;步骤七、利用公式Step 7. Use the formula${<span><span>[</span>[</span>\mathrm{<span><span>\&alpha;</span>\&alpha;</span>}<span><span>]</span>]</span>}_{\mathrm{<span><span>\&lambda;</span>\&lambda;</span>}}^{\mathrm{<span><span>t</span>t</span>}}<span><span>=</span>=</span>\frac{\mathrm{<span><span>\&theta;</span>\&theta;</span>}}{\mathrm{<span><span>l</span>l</span>}<span><span>\&CenterDot;</span>\&Center\; Dot;</span>\mathrm{<span><span>C</span>C</span>}}$计算获得待测溶液的旋光率；Calculate the optical rotation of the solution to be measured;式中，

右上角的t表示实验时温度，单位：℃，λ是旋光仪采用的单色光源的波长，单位：nm，θ为测得的旋光度，l为样品管的长度，单位：dm，C为溶液浓度，单位：g/100mL。In the formula,

The t in the upper right corner indicates the temperature during the experiment, unit: ℃, λ is the wavelength of the monochromatic light source used by the polarimeter, unit: nm, θ is the measured optical rotation, l is the length of the sample tube, unit: dm, C is Solution concentration, unit: g/100mL.5.根据权利要求4所述的非旋转式旋光溶液测量仪测量旋光溶液旋光率的方法，其特征在于，步骤七中λ为632.8nm。5. The method for measuring the optical rotation of an optically active solution with a non-rotating optically active solution measuring instrument according to claim 4, wherein in step seven, λ is 632.8nm.

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