技术领域technical field
本发明涉及生物芯片点样装置及方法。The invention relates to a biochip spotting device and method.
背景技术Background technique
在破解人类遗传奥秘、药物筛选等许多方面，生物芯片点样仪(或亦可称作“点样装置”)已成为必不可少的基本设备。因此，生物芯片点样仪技术水平的高低、性能指标的优劣在某种程度上也影响着生命科学研究的进程。仅就功能而言，无论哪种生物芯片点样仪，都是为了能够把所要研究或检验用的DNA，RNA，多肤，蛋白质及其他生物成分按所希望的矩阵点以高密度和高精度的方式固定在硅片、玻璃片或陶片等固相密质载体上，从而形成生物分子点阵。In many aspects such as deciphering the mysteries of human genetics and drug screening, the biochip spotting instrument (or also called "sample spotting device") has become an indispensable basic equipment. Therefore, the level of technology and performance indicators of biochip spotters also affect the progress of life science research to some extent. As far as the function is concerned, no matter what kind of biochip spotter, it is to be able to spot the DNA, RNA, polypeptide, protein and other biological components to be researched or tested in the desired matrix with high density and high precision. Immobilized on solid-phase dense carriers such as silicon wafers, glass wafers, or pottery wafers in a manner to form biomolecular lattices.
机械结构作为精度、速度、功能等的依托和执行者，在生物芯片点样仪中起着举足轻重的作用。合理的机械结构不仅可以为设备提供足够的刚度，使追求高精度和高速度成为可能，而且也应具有较高的空间利用率。到目前为止，生物芯片点样仪的机械结构一般都要涉及X/Y/Z轴三维运动。这样的机械结构布局比较复杂松散，承载生物芯片(基质)的载片台运动范围很大，空间利用率不高。这种结构的点样仪在使用中的实际占地面积很大，而且由于点样针的点样管路(现有的点样仪中，点样针与样品容器之间通常通过弯曲冗长的管路相连，该管路可简称为“点样管路”)很长，点样时产生气泡的可能性也比较大。另外，在点样过程中，点样针要不断运动，管路系统也不稳定，影响点样精度的压力变化比较大；这又导致了点样效率低，点样精度不高，生物芯片加工时间比较长，从而使得大多数科研机构难以接受。As the support and executor of precision, speed, function, etc., the mechanical structure plays a pivotal role in the biochip spotter. A reasonable mechanical structure can not only provide sufficient rigidity for the equipment, making it possible to pursue high precision and high speed, but also have a high space utilization rate. So far, the mechanical structure of biochip spotters generally involves three-dimensional movement of X/Y/Z axes. Such a mechanical structure layout is relatively complicated and loose, and the slide table carrying the biochip (substrate) has a large range of movement, and the space utilization rate is not high. The spotting instrument of this structure occupies a large area in use, and because the spotting pipeline of the spotting needle (in the existing spotting instrument, the lengthy length of bending is usually used between the spotting needle and the sample container). The pipeline is connected to each other, and the pipeline can be referred to simply as "sampling pipeline") is very long, and the possibility of generating air bubbles during sample application is relatively high. In addition, during the sampling process, the sampling needle needs to move continuously, the pipeline system is also unstable, and the pressure change that affects the sampling accuracy is relatively large; this in turn leads to low sampling efficiency, low sampling accuracy, and biochip processing The time is relatively long, which makes it difficult for most scientific research institutions to accept.
发明内容Contents of the invention
本发明的目的之一是提供一种生物芯片点样装置，其能够克服上述现有点样装置的某种或某些缺点。One of the objects of the present invention is to provide a biochip spotting device, which can overcome some or some shortcomings of the above-mentioned existing spotting devices.
根据本发明的滚筒式生物芯片点样装置包括：According to the drum type biochip spotting device of the present invention comprises:
具有外圆周表面的滚筒，其中生物芯片基质适于包覆在滚筒的外圆周表面上；A drum having an outer peripheral surface, wherein the biochip matrix is adapted to be coated on the outer peripheral surface of the drum;
滚筒驱动机构，用于转动滚筒；Drum drive mechanism for rotating the drum;
设置在滚筒上方并且安装有点样针阵列的点样针架；以及a sampling needle holder disposed above the drum and mounted with a sampling needle array; and
点样针架驱动机构，用于使点样针架相对于滚筒移动。The driving mechanism of the sampling needle holder is used to move the sampling needle holder relative to the roller.
本发明由于使用了滚筒表面作为生物芯片的承载面，因此大大提高了空间利用率，而且大大缩短了点样管路，减少了产生气泡的可能性(①第一次喷样的时候，管路中会残留气泡，若管路过长，去除气泡的时间就越长，遗留气泡的可能性也越大；②在换样品的时候，若管路过长，清洗的时间也越长，这也是气泡产生的一个重要因素；③管路过长，使得在点样过程中供样管路系统压力变化不稳定，也会导致管路产生气泡。本发明将点样管路直接集成入点样针架或点样针，通过点样针针头探入样品盒中取样，有效缩短了点样管路而避免了上述问题的存在，使得点样过程中管路系统压力稳定，故大大减小了产生气泡的可能性。)。同时，在纵向点样过程中，由于装有点样针的滑块并不移动，管路系统保持静止状态，影响点样精度的压力变化很小，故大幅提高了点样精度和点样效率。Because the present invention uses the roller surface as the bearing surface of the biochip, the space utilization rate is greatly improved, and the sampling pipeline is greatly shortened, which reduces the possibility of generating air bubbles (1. when spraying the sample for the first time, the pipeline If the pipeline is too long, the time to remove the bubbles will be longer, and the possibility of remaining bubbles will be greater; ②When changing samples, if the pipeline is too long, the cleaning time will be longer, which is also the cause of bubbles. An important factor; ③The pipeline is too long, so that the pressure change of the sample supply pipeline system is unstable during the sample pointing process, and it will also cause air bubbles in the pipeline. The present invention directly integrates the sample pointing pipeline into the pointing needle rack or point The sample needle probes into the sample box to take samples through the pointing needle, which effectively shortens the sample pointing pipeline and avoids the above-mentioned problems, making the pressure of the pipeline system stable during the sample pointing process, thus greatly reducing the possibility of air bubbles sex.). At the same time, during the longitudinal spotting process, since the slide block equipped with the spotting needle does not move, the pipeline system remains in a static state, and the pressure change that affects the spotting accuracy is small, so the spotting accuracy and spotting efficiency are greatly improved.
在本发明的一个优选实施例中，滚筒驱动机构包括直接与滚筒相连的滚筒伺服电机，用于使滚筒每次转动预设角度。滚筒与伺服电机直接连接的优点是传动误差小、定位精度高。In a preferred embodiment of the present invention, the roller driving mechanism includes a roller servo motor directly connected to the roller, which is used to rotate the roller by a preset angle each time. The advantage of the direct connection between the roller and the servo motor is that the transmission error is small and the positioning accuracy is high.
在本发明的另一个优选实施例中，点样装置还可以包括与滚筒相连的抽真空机构，其中滚筒的外圆周表面上设置有多个穿孔以使生物芯片基质能够被所述多个穿孔以真空吸附方式固定在滚筒的外圆周表面上。真空吸附固定方式使得生物芯片基质的更换更加方便快捷。In another preferred embodiment of the present invention, the spotting device may also include a vacuum mechanism connected to the drum, wherein a plurality of perforations are provided on the outer peripheral surface of the drum so that the biochip substrate can be covered by the plurality of perforations. It is fixed on the outer peripheral surface of the drum by means of vacuum adsorption. The vacuum adsorption fixation method makes the replacement of the biochip matrix more convenient and quick.
在本发明的又一个优选实施例中，滚筒的外圆周表面可以设有至少一个圆周槽。由于圆周槽对应于生物芯片基质的吸干区域，因此能够使点样针在执行吸干动作时避免损坏。In yet another preferred embodiment of the present invention, the outer circumferential surface of the drum may be provided with at least one circumferential groove. Since the circumferential groove corresponds to the blotted area of the biochip substrate, the sample needle can be prevented from being damaged during the blotted action.
在本发明的又一个优选实施例中，点样针架驱动机构可以包括：在滚筒上方安装的横向导轨；可滑动地安装在横向导轨上的竖向导轨，其中点样针架可滑动地安装在竖向导轨上；横向驱动电机，用于使竖向导轨沿横向导轨左右滑动；以及竖向驱动电机，用于使点样针架沿竖向导轨上下滑动。这种驱动方式使得点样针可以沿横向和竖向自由移动至所需位置。In yet another preferred embodiment of the present invention, the driving mechanism of the pointing needle rack may include: a horizontal guide rail installed above the roller; a vertical guide rail slidably installed on the horizontal guide rail, wherein the pointing needle rack is slidably installed On the vertical guide rail; the horizontal drive motor is used to make the vertical guide rail slide left and right along the horizontal guide rail; and the vertical drive motor is used to make the sampling needle holder slide up and down along the vertical guide rail. This type of drive allows the needle to move freely to the desired position both laterally and vertically.
在本发明的又一个优选实施例中，点样装置还可以包括设置在横向导轨下方并与滚筒并排设置的清洗槽、样品盒和液滴观测系统，其中当点样针架移动至清洗槽时，点样针架上的点样针阵列能够在清洗槽中得到清洗；当点样针架移动至样品盒时，点样针架上的点样针阵列能够从样品盒中吸取样品；当点样针架移动到液滴观测系统时进行预点样以观测预点样的液滴是否符合点样要求。这种并排布置方式既紧凑，又提高了点样效率。In yet another preferred embodiment of the present invention, the sample pointing device may also include a cleaning tank, a sample box, and a droplet observation system arranged under the transverse guide rail and arranged side by side with the roller, wherein when the sample pointing needle holder moves to the cleaning tank , the sampling needle array on the sampling needle holder can be cleaned in the cleaning tank; when the sampling needle holder moves to the sample box, the sampling needle array on the sampling needle holder can draw the sample from the sample box; When the sample needle holder moves to the liquid droplet observation system, pre-sampling is performed to observe whether the pre-spotted liquid droplets meet the requirements of the sample pointing. This side-by-side arrangement is both compact and improves sample placement efficiency.
在本发明的又一个优选实施例中，点样装置还可以包括安装在竖向导轨上的CCD(Charge-coupled Device)检测镜头以监视点样针阵列在生物芯片基质上的点样情况。在这种情况下，点样针架也可以通过滑块安装在竖向导轨上，并且CCD检测镜头安装在滑块上。(高速)CCD检测镜头的使用可以有效检测点样位置是否正确以及是否漏点样等。In yet another preferred embodiment of the present invention, the spotting device may also include a CCD (Charge-coupled Device) detection lens installed on the vertical guide rail to monitor the spotting situation of the spotting needle array on the biochip substrate. In this case, the sampling needle frame can also be installed on the vertical guide rail through the slider, and the CCD detection lens is installed on the slider. (High-speed) The use of CCD detection lens can effectively detect whether the spotting position is correct and whether spotting is missing.
本发明的另一个目的是提供一种生物芯片点样方法，其能够实现高速和高精度的点样。Another object of the present invention is to provide a biochip spotting method capable of high-speed and high-precision spotting.
根据本发明的生物芯片点样方法包括步骤：Biochip spotting method according to the present invention comprises steps:
将生物芯片基质真空吸附在滚筒外圆周表面上；The biochip matrix is vacuum-adsorbed on the outer peripheral surface of the drum;
通过点样针阵列将样品喷射在滚筒外圆周表面上的生物芯片基质上的生物芯片上；spraying the sample onto the biochip on the biochip substrate on the outer peripheral surface of the drum through the array of sampling needles;
以预设角度转动滚筒并重复执行上述喷射步骤。Turn the drum at a preset angle and repeat the spraying steps above.
本发明的方法由于采用可转动的滚筒外圆周表面作为承载生物芯片的表面，所以可以大幅度减小点样装置的体积。另外，在纵向点样过程中，由于装有点样针架的滑块并不移动，点样管路系统保持静止状态，影响点样精度的压力变化很小，故大幅提高了点样精度和点样效率，大大节省了点样时间，方便了生物芯片的制作。Because the method of the present invention adopts the outer peripheral surface of the rotatable cylinder as the surface for carrying the biochip, the volume of the sample spotting device can be greatly reduced. In addition, during the vertical sample pointing process, since the slide block equipped with the pointing needle holder does not move, the pointing pipeline system remains in a static state, and the pressure change that affects the pointing accuracy is small, so the pointing accuracy and pointing accuracy are greatly improved. The sampling efficiency greatly saves the sampling time and facilitates the production of biochips.
根据本发明的方法还优选包括步骤：使用CCD检测镜头检测点样针阵列在生物芯片上的样品喷射情况。如上所述，CCD检测镜头的使用可以有效检测点样位置是否正确以及是否漏点样等。The method according to the present invention preferably further includes the step of: using a CCD detection lens to detect the injection of the samples of the sampling needle array on the biochip. As mentioned above, the use of the CCD detection lens can effectively detect whether the position of the sample is correct and whether there is a sample missing.
附图说明Description of drawings
图1是根据本发明的滚筒式生物芯片点样装置的结构示意图；Fig. 1 is a schematic structural view of a drum-type biochip spotting device according to the present invention;
图2是图1的滚筒式生物芯片点样装置的局部结构示意图；Fig. 2 is a partial structural schematic diagram of the roller biochip spotting device of Fig. 1;
图3是滚筒的结构示意图；以及Fig. 3 is a schematic structural view of the drum; and
图4是生物芯片基质(膜)的示意图。Figure 4 is a schematic diagram of a biochip substrate (membrane).
具体实施方式Detailed ways
下面结合附图详细描述本发明的滚筒式生物芯片点样装置。本领域技术人员应当理解，下面描述的实施例仅是对本发明的示例性说明，而非用于对其作出任何限制。The drum-type biological chip spotting device of the present invention will be described in detail below with reference to the accompanying drawings. Those skilled in the art should understand that the embodiments described below are only illustrative illustrations of the present invention, and are not intended to limit it in any way.
图1示出了本发明的滚筒式生物芯片点样装置，其包括滚筒1和点样针架8。图4所示的生物芯片基质16可以包覆在滚筒1的外圆周表面上。点样针架8上通常安装有阵列形式的点样针11。FIG. 1 shows a roller-type biochip spotting device of the present invention, which includes a roller 1 and a spotting needle rack 8 . The biochip matrix 16 shown in FIG. 4 can be coated on the outer peripheral surface of the drum 1 . The sampling needle frame 8 is usually equipped with sampling needles 11 in the form of an array.
点样针架8或点样针11上还可以设置有样品暂储腔(未具体示出)，用于暂时存储点样针8从后文提及的样品盒4中所吸取的样品，以如随后所述那样将其中的样品通过点样针11在生物芯片基质16上的生物芯片18上喷射形成相应的生物分子点阵。在这种情况下，与传统点样仪的从样品容器至点样针的弯曲冗长的点样管路相比，本发明的从设置在点样针架8或点样针11上的样品暂储腔至点样针的点样管路既短又直，这如前所述大大减小了点样管路中产生气泡的可能性。A sample temporary storage cavity (not specifically shown) may also be provided on the sampling needle frame 8 or the sampling needle 11 for temporarily storing the sample drawn by the sampling needle 8 from the sample box 4 mentioned later, so as to As will be described later, the samples are sprayed on the biochip 18 on the biochip substrate 16 through the sampling needle 11 to form corresponding biomolecular arrays. In this case, compared with the tortuous and lengthy sampling pipeline from the sample container to the sampling needle of the traditional sampling instrument, the sample temporarily arranged on the sampling needle holder 8 or the sampling needle 11 of the present invention The application line from the reservoir to the application needle is short and straight, which, as mentioned earlier, greatly reduces the possibility of air bubbles in the application line.
图1还示出了在滚筒1上方安装的横向导轨6。竖向导轨10可滑动地安装在横向导轨6上，通过安装在横向导轨6上的横向驱动电机12的驱动，竖向导轨10能够沿横向导轨6左右移动。点样针架8通过滑块13(参见图2)可滑动地安装在竖向导轨10上，通过安装在竖向导轨10上的竖向驱动电机9的驱动，滑块13能够沿竖向导轨10上下移动。FIG. 1 also shows the transverse rails 6 installed above the drum 1 . The vertical guide rail 10 is slidably installed on the transverse guide rail 6 , driven by the transverse driving motor 12 installed on the transverse guide rail 6 , the vertical guide rail 10 can move left and right along the transverse guide rail 6 . The sampling needle frame 8 is slidably installed on the vertical guide rail 10 through the slide block 13 (see FIG. 2 ), and driven by the vertical drive motor 9 installed on the vertical guide rail 10, the slide block 13 can move along the vertical guide rail 10. 10 Move up and down.
横向驱动电机12采用伺服电机，可以通过例如精密滚珠丝杠(未具体示出)连接于竖向导轨10。横向驱动电机12与精密滚珠丝杠之间则可以采用联轴器(同样未示出)进行连接。The horizontal drive motor 12 is a servo motor, and can be connected to the vertical guide rail 10 through, for example, a precision ball screw (not specifically shown). A shaft coupling (also not shown) can be used to connect the transverse drive motor 12 and the precision ball screw.
图1还示出了布置在滚筒1左侧的滚筒驱动电机2。滚筒驱动电机2也采用伺服电机，并直接连接于滚筒1。FIG. 1 also shows a drum drive motor 2 arranged on the left side of the drum 1 . The drum driving motor 2 also adopts a servo motor and is directly connected to the drum 1 .
此外，在图1中的滚筒1的左侧，还分别示出了与滚筒1并排设置的清洗槽3、样品盒4和频闪装置5。清洗槽3用于以例如超声波形式清洗移动至其中的点样针11。样品盒4用于容纳生物样品例如DNA或RNA。当点样针架8移动至样品盒时，点样针架8上的点样针11能够从样品盒4中吸取生物样品。取样完成后，点样针架8移动到液滴观测系统5进行预点样，在其中包含的例如频闪装置的辅助下观测预点样的液滴是否符合点样要求。这种包含频闪装置的液滴观测系统对预点样液滴的大小、数量、飞行角度等信息进行检测，通过图像处理自动判断喷液效果是否合格；其已为本领域所熟知，在此不再进一步详细描述其结构和工作原理。In addition, on the left side of the drum 1 in FIG. 1 , a cleaning tank 3 , a sample box 4 and a strobe device 5 arranged side by side with the drum 1 are respectively shown. The cleaning tank 3 is used to clean the sampling needle 11 moved therein, for example, in the form of ultrasonic waves. The sample cassette 4 is used to contain biological samples such as DNA or RNA. When the sampling needle holder 8 moves to the sample box, the sampling needle 11 on the sampling needle holder 8 can absorb the biological sample from the sample box 4 . After the sampling is completed, the sample pointing needle rack 8 moves to the droplet observation system 5 for pre-pointing, and with the assistance of a strobe device included therein, it is observed whether the pre-pointed liquid droplets meet the pointing requirements. This liquid drop observation system including stroboscopic device detects information such as the size, quantity, and flight angle of the pre-spotted liquid droplets, and automatically judges whether the liquid spray effect is qualified through image processing; it is well known in the art, and here Its structure and working principle will not be described in further detail.
图1还示出了安装在竖向导轨10上的CCD检测镜头7。当然，CCD检测镜头7也可以直接安装在图2示出的滑块13上。CCD检测镜头7用于检测点样针11在生物芯片基质16上的生物芯片18上的点样情况。CCD检测镜头7还可以与控制系统(未具体示出)相连以根据检测情况实时监控点样针11的点样情况。如上所述，CCD检测镜头7既可以安装成随点样针架8一起上下或左右移动，也可以安装成仅随竖向导轨10一起左右横向移动，只要其能够实际检测到点样针的点样情况即可。FIG. 1 also shows the CCD detection lens 7 installed on the vertical guide rail 10 . Of course, the CCD detection lens 7 can also be directly installed on the slider 13 shown in FIG. 2 . The CCD detection lens 7 is used to detect the spotting situation of the sampling needle 11 on the biochip 18 on the biochip matrix 16 . The CCD detection lens 7 can also be connected with a control system (not specifically shown) to monitor the sample application by the sample needle 11 in real time according to the detection conditions. As mentioned above, the CCD detection lens 7 can be installed to move up and down or left and right together with the sampling needle frame 8, and can also be installed to only move left and right along with the vertical guide rail 10, as long as it can actually detect the point of the sampling needle. That's fine.
图3示出了本发明的滚筒1。如图所示，滚筒1的外圆周表面上设置有多个圆周槽14和多个穿孔15。图4所示的生物芯片基质16可以是合适吸附性材料制成的膜片。生物芯片基质16上形成有多列生物芯片18，每列生物芯片18之间由假想虚线所表示的吸干区域17隔开。点样时，生物芯片基质16包覆在滚筒1的外圆周面上，并且生物芯片基质16的吸干区域17与滚筒1的圆周槽14重合。在与滚筒1相连的抽真空装置(未具体示出)所产生的真空吸力的作用下，穿孔15将生物芯片基质16牢牢吸附在滚筒1的外圆周表面上。Figure 3 shows the drum 1 of the invention. As shown in the figure, the outer peripheral surface of the drum 1 is provided with a plurality of circumferential grooves 14 and a plurality of perforations 15 . The biochip matrix 16 shown in FIG. 4 can be a membrane made of suitable adsorptive material. A plurality of rows of biochips 18 are formed on the biochip substrate 16, and each row of biochips 18 is separated by a blotted area 17 indicated by a phantom dotted line. When applying samples, the biochip matrix 16 is coated on the outer peripheral surface of the drum 1 , and the blotted area 17 of the biochip matrix 16 coincides with the circumferential groove 14 of the drum 1 . Under the action of vacuum suction generated by a vacuum device (not specifically shown) connected to the drum 1 , the perforations 15 firmly adsorb the biochip matrix 16 on the outer peripheral surface of the drum 1 .
下面详细描述本发明的点样装置的工作原理。The working principle of the sample spotting device of the present invention will be described in detail below.
首先，生物芯片基质16被如上所述以真空吸附方式固定在滚筒1的外圆周表面上，从而可以随滚筒2一起转动。First, the biochip matrix 16 is fixed on the outer peripheral surface of the drum 1 by vacuum adsorption as described above, so as to be able to rotate together with the drum 2 .
随后启动点样装置，在横向驱动电机12的作用下，先使竖向导轨10连同其上的点样针架8沿横向导轨6运动到清洗槽3的位置，由竖向驱动电机9控制点样针架8沿竖向导轨10向下运动，使点样针11在清洗槽3中完成例如超声波清洗。清洗完毕后使点样针架8再沿横向导轨6向右运动到样品盒4的位置以使点样针11吸取样品盒4中的样品例如DNA。Then start the sample pointing device, under the action of the horizontal drive motor 12, first make the vertical guide rail 10 move along the horizontal guide rail 6 to the position of the cleaning tank 3 along with the sample pointing needle frame 8 on it, and the point is controlled by the vertical drive motor 9 The needle holder 8 moves downward along the vertical guide rail 10 , so that the sample needle 11 completes, for example, ultrasonic cleaning in the cleaning tank 3 . After cleaning, the sampling needle frame 8 is moved to the right along the transverse guide rail 6 to the position of the sample box 4 so that the sampling needle 11 draws the sample such as DNA in the sample box 4 .
然后，点样针架移动到液滴观测系统5进行预点样，并在频闪装置的帮助下观测预点样的液滴是否符合点样要求。如果预点样效果合格，则控制点样针架8运动到滚筒1上的最左侧的圆周槽14的位置，由竖向驱动电机9控制点样针11向下运动直至接触生物芯片基质16上的吸干区域17。在前述吸取样品或预点样过程中点样针11上会因为吸附或喷射而附着一些残余样品，如果不将其吸干，点样针11上的这些残余样品会影响随后的点样精度。由于生物芯片基质16具有良好的吸附性，因此可将点样针11上的残余样品吸干。又由于如上所述吸干区域17重合或覆盖在圆周槽14上(即悬空设置)，因此点样针11在接触吸干区域17时，只接触生物基质膜16本身，不会直接接触滚筒1的主体而损坏点样针11。Then, the sample pointing needle holder moves to the liquid drop observation system 5 for pre-pointing, and with the help of the strobe device, it is observed whether the pre-pointing liquid drops meet the pointing requirements. If the pre-spotting effect is qualified, the control pointing needle holder 8 moves to the position of the leftmost circumferential groove 14 on the cylinder 1, and the vertical drive motor 9 controls the pointing needle 11 to move downward until it contacts the biochip matrix 16 17 on the blotting area. During the aforementioned sampling or pre-sampling process, some residual samples will adhere to the sampling needle 11 due to adsorption or spraying. If they are not blotted dry, these residual samples on the sampling needle 11 will affect the subsequent sampling accuracy. Since the biochip matrix 16 has good adsorption properties, the residual sample on the sampling needle 11 can be blotted dry. And because the blotted area 17 overlaps or covers on the circumferential groove 14 as mentioned above (i.e. suspended setting), so when the sampling needle 11 contacts the blotted area 17, it only contacts the biological matrix membrane 16 itself, and will not directly contact the cylinder 1 The main body of the sample needle 11 is damaged.
点样针11吸干动作完成后，控制针架8沿横向导轨6运动到生物芯片18的起始点样位置，采用非接触式喷印法(类似喷墨打印机的原理)将样品通过点样针11喷射到生物芯片18的预定位置上以形成所期望的生物分子点阵。在点样的同时，高速CCD检测镜头7检测生物芯片18上的点样情况或状态。每点完一个生物芯片18，滚筒驱动电机2控制滚筒1转动预设角度以对应于下一个生物芯片18，点样针11继续对该生物芯片18进行点样。滚筒1每转一圈完成生物芯片基质16上一列生物芯片18的纵向点样。After the drying action of the sampling needle 11 is completed, the needle holder 8 is controlled to move along the transverse guide rail 6 to the initial sampling position of the biochip 18, and the sample is passed through the sampling needle by using a non-contact printing method (similar to the principle of an inkjet printer). 11 is sprayed onto the predetermined position of the biochip 18 to form the desired biomolecule lattice. Simultaneously with spotting, the high-speed CCD detection lens 7 detects the spotting situation or state on the biochip 18 . Every time a biochip 18 is spotted, the roller drive motor 2 controls the roller 1 to rotate a preset angle to correspond to the next biochip 18 , and the sampling needle 11 continues to apply samples to the biochip 18 . Each rotation of the drum 1 completes the longitudinal spotting of a row of biochips 18 on the biochip matrix 16 .
然后，由横向驱动电机12控制点样针架8沿水平方向向右运动到生物芯片基质16的第二个吸干区域以进行点样针11的吸干。点样针11吸干动作完成后，控制点样针架8沿水平方向向右运动一个点阵距离到达另一列生物芯片18，开始这一列生物芯片18的点样。如此循环，直至所有列的生物芯片18点样完毕。完成点样的生物芯片基质16从滚筒1上取下，换一张新的生物芯片基质16吸附在滚筒1外圆周表面上，为下一轮的点样做好准备。同时，点样针架8快速返回清洗槽3位置进行点样针11的清洗，准备开始下一轮的清洗、取样、吸干和点样动作。Then, controlled by the horizontal drive motor 12 , the sampling needle frame 8 moves rightward in the horizontal direction to the second blotted area of the biochip substrate 16 to blot the sampled needle 11 . After the drying action of the sampling needle 11 is completed, the sampling needle frame 8 is controlled to move to the right in the horizontal direction for a lattice distance to reach another column of biochips 18, and the sampling of this column of biochips 18 begins. This cycle is repeated until the biochips 18 of all columns are printed. The finished biochip substrate 16 is removed from the drum 1, and a new biochip substrate 16 is adsorbed on the outer peripheral surface of the drum 1 to prepare for the next round of sample placement. At the same time, the sampling needle rack 8 quickly returns to the position of the cleaning tank 3 to clean the sampling needle 11, and is ready to start the next round of cleaning, sampling, drying and sample application.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310227100.3ACN103278365B (en) | 2013-06-08 | 2013-06-08 | Drum type biochip spotting device and method thereof |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310227100.3ACN103278365B (en) | 2013-06-08 | 2013-06-08 | Drum type biochip spotting device and method thereof |
| Publication Number | Publication Date |
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| CN103278365A CN103278365A (en) | 2013-09-04 |
| CN103278365Btrue CN103278365B (en) | 2015-06-24 |
| Application Number | Title | Priority Date | Filing Date |
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| CN201310227100.3AExpired - Fee RelatedCN103278365B (en) | 2013-06-08 | 2013-06-08 | Drum type biochip spotting device and method thereof |
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| CN (1) | CN103278365B (en) |
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