CN103168781A - Compound sterilizing agent containing fluopicolide and fludioxonil and application of sterilizing agent - Google Patents

Abstract

Translated fromChinese

本发明公开了一种复配杀菌剂及其应用。所述复配杀菌剂由氟吡菌胺和咯菌腈组成，其质量比为1～9∶1～9，优选质量比为1～7∶1～5，更优选质量比为1～5∶1～3。本发明提出的复配杀菌剂可应用于防治蔬菜霜霉病、晚疫病、灰霉病以及黄瓜根腐病和辣椒疫病等植物病害。该复配杀菌剂速效性和持效性好，作用机制独特，作用位点多，杀菌谱广，生物安全性高，且剂制备工艺简单，成本低，经济效益显著。The invention discloses a compound fungicide and its application. The compound bactericide is composed of fluopyram and fludioxonil, and its mass ratio is 1-9:1-9, preferably 1-7:1-5, more preferably 1-5: 1~3. The compound fungicide proposed by the invention can be applied to control plant diseases such as vegetable downy mildew, late blight, gray mold, cucumber root rot and pepper blight. The compound bactericide has good quick-acting and long-lasting effects, unique action mechanism, multiple action sites, wide bactericidal spectrum, high biological safety, simple preparation process, low cost and remarkable economic benefits.

Description

The composite bactericide of a kind of containing fluopicolide and fludioxonil and application thereof

Technical field

The invention belongs to pesticide field, be specifically related to composite bactericide and the application thereof of a kind of containing fluopicolide and fludioxonil.

Background technology

Vegetables oomycetes disease be infected by the Mastigomycotina oomycetes due to, wherein, downy mildew mould take epidemic disease, the rotten oomycetes such as mould are as important pathogen, the vegetable crops such as main harm Cruciferae, Solanaceae, Curcurbitaceae, cause damping off, pythium rot, epidemic disease, cotton disease, downy mildew and root rot etc., cause the significantly underproduction even to have no harvest^[1]Especially downy mildew and phytophthora short incubation period, then infect often, germ can develop rapidly and cause disease popular within the season of growth of plant, causes the heavy losses of agricultural production.Can cause cucumber plant all dead in several days as cucumber downy mildew^[2]

Botrytis cinerea (Botrytis cinerea) causes the gray mold of most crops, can infect the multiple fruit trees and vegetables such as tomato, eggplant, cucumber, custard squash, grass are mould, grape, Kidney bean.Gray mold is a kind of global important disease, is a kind of gas borne disease, the flower of crop, really, leaf and stem all can fall ill^[3]This disease generally occurs throughout the country, and in rising trend.The ash arrhizus bacteria host range is very extensive, not only can infect tomato and also can infect approximately 235 kinds of crops such as eggplant, cucumber, capsicum, strawberry, grape, garlic, romaine lettuce, oranges and tangerines^[4-6]

Cucumber root rot is often caused by multiple pathogen Combined Infection, as fusarium pine root fungus (Fusarium solani), Phomopsis pine root fungus (Phomopsis sp), rotten mould pine root fungus (Pythium myriotylum) and phytophthora root rot bacterium (Phytophthora drechsleri) etc., all use 30% to dislike 800 times of liquid of mould Lingshui Spring agent, 650 times of liquid irrigating roots of the 3% mould first frost aqua of evil.In addition, also 250 times of liquid of available 12.5% carbendazim soluble powder, 60% good fortune of fusarium root rot and Phomopsis root rot. 700 times of liquid of first sulphur wetting powder, 1000 times of liquid irrigating roots of 50% chlorobromide isocyanuric acid soluble powder.Rotten mould root rot and phytophthora root rot be 600 times of liquid of available 25% metalaxyl wetting powder also, and 600 times of liquid of 50% fluorine mofetil aluminium wetting powder etc. are filled with root.Fill with in case of necessity one twice 200 milliliters of left and right of every strain consumption when filling with root again.Above-mentioned effect of chemical control is often very poor, demands developing new and effective medicament in production urgently, improves it to the control efficiency of cucumber root rot.

Use chemical agent and be the most effectively means of controlling plant diseases, but the long-term applicable single bactericide of high dose continuously, easily cause the problems such as residual, the environmental pollution of medicament and the development of drug resistance fungi.Reasonably chemical bactericide is composite or be mixed and have the expansion fungicidal spectrum, improve control efficiency, extend dispenser interval, reduce dosage, reduce poisoning, reduce residual, delay disease fungus drug resistance and the characteristics such as drug-fast generation and development.Bactericide compounded is one of effective ways of addressing the above problem.

The benzamide series bactericidal agent; representative products is fluopicolide; Plasmopara viticola, bacterium of downy mildew of cucumber, phytophthora infestans, tomato late blight bacterium and downy mildew of lettuce bacterium etc. are had than high bacteriostatic activity; its mechanism of action is to disturb the combination of class spectrin and other components; destroy the stability of cytoskeleton; the release, sporangial formation and sprouting and the mycelial growth that suppress zoospore have protection and therapeutic action^[7,8]68.75% silver medal method profit SC is the complex preparation of fluopicolide and Propamocarb, and the Oomycete diseases such as downy mildew are had remarkable preventive effect.

Fludioxonil is a kind of pyroles bactericide of novel structure, main by suppressing the phosphorylation of MAPK/histidine kinase in the conduction of disease fungus infiltration signal, cause the many alcohols such as glycerine in thalline to increase, thereby suppress conidia germination, germ tube elongation and mycelial growth, finally cause germ dead^[9]The pyroles bactericide is a novel non-internal-absorting wide-spectrum bactericide of class of being developed by the antibiotic derivative of nitre pyroles that pseudomonas produces^[10,11], mainly comprising fludioxonil, fenpiclonil and three kinds of fluorine acid imide, this series bactericidal agent is chosen as by EPA the product that is free from risk due to characteristics such as efficient, safety, low consumptions^[12]It is reported, fludioxonil is to penicillium expansum (Penicillium expansum), Botrytis cinerea (Botrytis cinerea)^[13], rhizoctonia (Rhizoctonia spp.)^[14], chain lattice spore (Alternaria spp.)^[15]Mycelial growth and spore germination Deng pathogen all have inhibitory action preferably.Fludioxonil mainly passes through seed treatment or soil treatment controlling plant diseases in China, but fludioxonil was developed as wetting powder by Syngenta Co.,Ltd over the past two years, is used for spraying preventing and treating tomato or flowers gray mold, and shows excellent control efficiency^[16]

So far there is not yet the report that utilizes fluopicolide and fludioxonil development mixture control cucumber downy mildew, potato/tomato late blight, eliminating vegetable botrytis and the diseases such as cucumber root rot and capsicum epidemic disease.

List of references:

[1] department is state, Liu Junli. the chemical control present situation of oomycetes disease and control strategy [J]. and agricultural chemicals, 2000,39 (2): 7-10.

[2] Dong Jingao chief editor. Agricultural Plant Pathology. the north this [M]. Chinese agriculture publishing house, 2001.5.

[3] Kang Lijuan, Zhang Xiaofeng, Wang Wenqiao, etc. the pesticide resistance of botrytis cinerea and fitness test [J]. Pesticide Science journal, 2003,2:39-42.

[4] wangdan. the comparative studies of Different Crop ash arrhizus bacteria biological property and the biological and ecological methods to prevent plant disease, pests, and erosion of graw mold of tomato research [D]. Sichuan: Sichuan Agricultural University, 2008.

[5] Li Baoju. the research of greenhouse tomato gray mold regularty of epidemic. see: the chief editors such as Feng Lanxiang. Tomato in China damage by disease and insect and the anti-progress of making a study of subjects thereof. Beijing: Chinese agriculture publishing house, 1998,31-35.

[6] Zhang Congyu, Zhang Juquan, Hu Nengbing, etc. botrytis cinerea host range and Virulence research [J]. Anhui Technology Normal College's journal, 2003,17 (3): 239-242.

[7] Liu Changling. the complete works of bactericide volume [M] of world pesticide. Chemical Industry Press, 2006:203-205.

[8]Takeshi?O，Terumasa?K，Shigeru?M.Development?of?a?novel?fungicide，cyazofamid[J].Pesticide?Science，2004，29：136-138.

[9]Hagiwara?D，AsanoY，Marui?J，etal.Transcriptional?profiling?for?Aspergillus?nidulans?HogA?MAPK?signaling?pathway?in?response?to?fludioxonil?and?osmotic?stress[J].Fungal?Genetics?and?Biology，2009，46(11)：868-878.

[10]Gehmann?K，Nyfeler，R，Leadbeater，A?J，etal.A?new?phenylpyrrole?fungicide?for?broad-spectrum?disease?control[A].Brighton?Crop?Prot.Conf.on?Pests?and?Dis[C].UK，1990：369-376.

[１１]Errampalli，D.Effect?of?fludioxonil?on?germination?and?growth?of?Penicillium?expansum?and?decay?in?apple?cVs.Empire?Gala[J].Crop?Protection，2004，23：811-817.

[12] Yan Xiaomin, Ning Binke, Wang Lieping, etc. new type bactericide fluorine fludioxonil and New Progresses of R ﹠ D thereof [J]. world pesticide, 2010,12 (3): 32-38.

[13]Leroux，P.Recent?developments?in?the?mode?of?action?of?fungicides[J].Pesticide?Management?Science，1996，47(2)：191-197.

[14]Iacomi-Vasilescu?B，Avenot?H，Bataiué-Simoneau?N，etal.In?vitro?fungicide?sensitivity?of?Alternaria?species?pathogenic?to?crucifers?and?identification?of?Alternaria?brassicicola?field?isolates?highly?resistant?to?both?dicarboximides?and?phenylpyrroles[J].Crop?Protection，2004，23：481-488.

[15]Hamada?M?S，Yin?Y?N，Ma?Z?H.Sensitivity?to?iprodione，difenoconazole?and?fludioxonil?of?Rhizoctonia?cerealis?isolates?collected?from?wheat?in?China[J].Crop?Protection，2011，30：1028-1033.

[16] Ji Junjian, Wang Wenqiao, Zhang Xiaofeng, etc. dispenser and indoor fungicide tame are on the impact [J] of tomato Botrytis cinerea on fludioxonil susceptibility continuously. Pesticide Science journal, 2012,14 (1): 35-41.

Summary of the invention

For the weak point that existing composite bactericide exists, the invention provides the composite bactericide of control vegetables downy mildew, late blight, gray mold and the diseases such as cucumber root rot and capsicum epidemic disease of a kind of novel, efficient, safety, applied range.

Composite bactericide provided by the present invention is comprised of fluopicolide and fludioxonil, and its mass ratio is 1～9: 1～9.

Preferably, the mass ratio of fluopicolide and fludioxonil is 1～7: 1～5.

More preferably, the mass ratio of fluopicolide and fludioxonil is 1～5: 1～3.

Most preferably, the mass ratio of fluopicolide and fludioxonil is 1: 1.

The present invention also provides a kind of compound sterilizing agent formulation.

Compound sterilizing agent formulation provided by the present invention is comprised of acceptable auxiliary agent on above-mentioned composite bactericide and pesticidal preparations.Wherein, described composite bactericide can account for 20% of the described composite bactericide quality of the pharmaceutical preparations.

Described compound sterilizing agent formulation can be the pesticidal preparations of any one formulation, one or more formulations in preferred following formulation: soluble powder (SP), soluble granula (SG), water dispersible granules (WG), aqua (AS), wetting powder (WP), soluble liquid (SL), missible oil (EC), aqueous emulsion (AE), microemulsion (ME) and suspending agent (SC).

Above-mentioned formulation all can adopt method preparation well-known in the art, normally with active substance and auxiliary agent, filler, fully mixed dissolution or grinding or granulation, making preparation uses, the auxiliary agent that uses in fungicide preparation of the present invention comprises that dispersant, wetting agent, binding agent, thickener, anti-foaming agent, disintegrant, filler etc. and other are of value to the material of active substance stable and performance drug effect in preparation, be not particularly limited, concrete component and consumption are determined by test.

Described compound sterilizing agent formulation, its composition specifically can comprise the component of following mass parts:

1～9 part of fluopicolide

1～9 part of fludioxonil

Filler is appropriate

Described compound sterilizing agent formulation also can further comprise the component of following mass parts:

Said components is not 0 simultaneously.

When described compound sterilizing agent formulation was wetting powder, its composition comprised the component of following mass parts:

Described wetting powder also can further comprise the component of following mass parts: 0～8 part of dispersant, but do not comprise 0 part.

Its preparation process is as follows: according to said ratio, various compositions through mix and blend, are entered one-level and pulverize (grain fineness reaches 80 orders); After carrying out mix and blend again, enter secondary pulverizing (grain fineness reaches 325 orders) and make finished product.

When described compound sterilizing agent formulation was water dispersible granules, its composition comprised the component of following mass parts:

Described water dispersible granules also can further comprise the component of following mass parts: 0～8 part of dispersant, but do not comprise 0 part.

Its preparation process is: according to said ratio, fluopicolide, fludioxonil, dispersant, wetting agent and filler are passed through mix and blend; air-flow crushing obtains wetting powder; then wetting powder and water (or with binding agent) are added in kneader simultaneously and mediate; make plastic material; send this material to Squeezinggranulator at last; carry out granulation, obtain the water dispersible granules product by dry, screening.

Described dispersant is one or more the mixture in lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt (NNO), 3-oleoyl amino-2-methoxy benzenesulfonic acid sodium (LS), sanlose (CMC), alkylphenol polyoxyethylene, ethylene glycol monobutyl ether, polyoxyethylene alkyl ether and neopelex.

Described lignin sulfonic acid sodium salt specifically can be: sodium lignin sulfonate (M-14), desugar sodium lignin sulfonate (M-9), lignosulfite.Described alkylphenol polyoxyethylene specifically can be: octyl phenol polyoxy ethene (10) ether (OP-10), polyoxyethylene nonylphenol ether.

Described wetting agent is at least a in sodium alkyl benzene sulfonate, alkyl benzene calcium sulfonate, Negel, fatty alcohol-polyoxyethylene ether (JFC), alkylphenol-polyethenoxy base formaldehyde condensation products sulfonate (SOPA), wgwin D30, soapberry powder and bay alcohol radical sodium sulphate.

Described sodium alkyl benzene sulfonate specifically can be neopelex.Described Negel specifically can be nekal (Nekal BX).

Described filler is one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite and bentonite.

Described binding agent is one or more in cyclodextrin, carboxymethyl cellulose, gum Arabic and polyvinyl alcohol.

Described thickener is one or more in xanthans, CMC, methylcellulose, Magnesiumaluminumsilicate and polyvinyl alcohol.

Described anti-foaming agent is silicone anti-foaming agent M10 or anti-foaming agent C.

Described disintegrant is one or more in bentonite, calcium chloride, anhydrous sodium sulfate, kaolin, diatomite, ammonium sulfate, urea, sodium phosphate trimer and sodium pyrophosphate.

The present invention also provides the application of above-mentioned bactericidal composition and compound sterilizing agent formulation.

The application of bactericidal composition provided by the present invention and compound sterilizing agent formulation is its application in controlling plant diseases.Described plant disease refers in particular to the disease of melon and fruit and/or vegetables.

Described plant disease comprises oomycetes disease, gray mold and root rot; Described oomycetes disease comprises downy mildew, late blight, capsicum epidemic disease etc.Specifically can be cucumber downy mildew, potato late blight, tomato late blight, gray mold of cucumber, graw mold of tomato, capsicum epidemic disease and cucumber root rot etc.

Beneficial effect of the present invention is:

20% fluopicolide fludioxonil WP180g a.i./hm of the present invention²(1000 times of liquid), 200g a.i./hm²(900 times of liquid), 250g a.i./hm²(700 times of liquid) spraying control cucumber downy mildew, potato/tomato late blight, the effect of cucumber/graw mold of tomato and capsicum epidemic disease is reached 80.1%～92.6%; The effect of filling with root control cucumber root rot reaches 49.1%～63.7%.This composite bactericide quick-acting and lasting effect are good, and mechanism of action is unique, and action site is many, can effectively delay the drug-fast generation of pathogen and development.Preparation technology is simple for this complex preparation, and cost is low, remarkable in economical benefits, and the diseases prevention spectrum is wide, and biological safety is high.

Embodiment

The present invention will be described below by specific embodiment, but the present invention is not limited thereto.

Experimental technique described in following embodiment if no special instructions, is conventional method; Described reagent and material if no special instructions, all can obtain from commercial channels.

Medicine in embodiment and experimental example: the 96% former medicine of fludioxonil, 50% fludioxonil WP are all available from first just reaching (China) Investment Co., Ltd; The 96% former medicine of fluopicolide, 68.75% fluopicolide Propamocarb SC are all available from Bayer Cropscience (China) Co., Ltd; 50% dimethomorph WP, 50% Boscalid WG are all available from German BASF AG." % " in following embodiment is the quality percentage composition.

The embodiment of compound sterilizing agent formulation

Embodiment 1: the wetting powder of composite bactericide

Preparation: 20% fluopicolide fludioxonil WP (1: 1, w/w): get the 96% former medicine 10.42kg of fluopicolide, the 96% former medicine 10.42kg of fludioxonil, add 2kgM-9,1.5kgCMC, 5kg neopelex, 70.67kg fine particle calcium carbonate, through mix and blend, enter one-level and pulverize (grain fineness reaches 80 orders); Go again after mix and blend, enter secondary and pulverize (grain fineness reaches 325 orders), be packaged as product.

Embodiment 2-6: wetting powder

The preparation method is with embodiment 1, and different is the component that table 1 is listed.

Table 1 embodiment 2-6 component

Embodiment 1-6 composite bactericide fluopicolide fludioxonil WP quality testing

1) active constituent content: utilize the content of liquid chromatography for measuring fluopicolide and fludioxonil, result shows that the content of active ingredient in embodiment 1-6 fluopicolide fludioxonil WP is all greater than 20.00%.

2) wettability determination: carry out according to the GB/T5451-2001 agriculture wetting powder wettability determination method that wraps with straw.The moistening time of embodiment 1-6 fluopicolide fludioxonil WP is 55s.

3) mensuration of suspensibility: carry out according to GB/T14825-2006 pesticide wettable suspensibility assay method.The suspensibility of embodiment 1-6 fluopicolide fludioxonil WP reaches more than 85%.

4) mensuration of fineness: carry out according to the wet screening in GB/T16150-1995 pesticide wettable fineness assay method.Embodiment 1-6 fluopicolide fludioxonil WP by 325 mesh standard sieves (being particle diameter＜44 μ m) reaches 98.5%.

5) mensuration of moisture: carry out according to azeotropic distillation method in GB/T1600-2001 agricultural chemicals moisture assay method.Moisture in embodiment 1-6 fluopicolide fludioxonil WP≤1.8%.

6) mensuration of mobility: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.Result shows that the flow index of embodiment 1-6 fluopicolide fludioxonil WP is 1.

7) mensuration of foaming characteristic: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.The foaming volume of embodiment 1-6 fluopicolide fludioxonil WP is 5mL, meets national standard.

8) mensuration of thermodynamic stability: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.After the storage of embodiment 1-6 fluopicolide fludioxonil WP heat, the resolution ratio of active ingredient≤5%, meet national standard.

The water dispersible granules of embodiment 7 composite bactericides

Preparation: 20% fluopicolide fludioxonil WP (1: 1; w/w): get the 96% former medicine 10.42kg of fluopicolide; the 96% former medicine 10.42kg of fludioxonil; add NNO2kg; CMC1.5kg; neopelex 5kg; white carbon 63.67kg; through mix and blend, but air-flow crushing obtains wet-milling, mediates in kneader but wet-milling is added simultaneously with 2kg cyclodextrin and 5kg water; make plastic material; send this material to Squeezinggranulator at last, carry out granulation, obtain the water dispersible granules product by dry, screening.

Embodiment 8-12: the water dispersible granules of composite bactericide

The preparation method is with embodiment 7, and different is the listed component of table 2.

Table 2 embodiment 8-12 component

Embodiment 7-12 composite bactericide fluopicolide fludioxonil WG quality testing

1) active constituent content measuring: the content that utilizes liquid chromatography for measuring fluopicolide and fludioxonil.Result shows, in embodiment 7-12 in fluopicolide fludioxonil WG the content of active ingredient all greater than 20.00%.

2) determination of moisture: carry out according to azeotropic distillation method in GB/T1600-2001 agricultural chemicals moisture assay method.Result shows, the moisture in embodiment 7-12 fluopicolide fludioxonil WG≤1.8%.

3) dispersed mensuration: adopt the dispersiveness of long tube test method determination embodiment 7-12 fluopicolide fludioxonil WG, result is 0.1mL for the sediments of pipe bottom.

4) wettability determination: adopt the measuring graduates test method determination.Result shows, the wetability of embodiment 7-12 fluopicolide fludioxonil WG is good.

5) disintegrative is measured: to 100mL tool plug graduated cylinder (the interior high 22.5cm that contains 90mL distilled water, internal diameter 28cm) add sample particle (0.5g under 25 ℃, 250～1410 μ m), clamp afterwards the middle part of graduated cylinder, clog bung, with the speed of 8r/min around central rotation, until sample disintegration fully in water.Result shows, the disintegration time of embodiment 7-12 fluopicolide fludioxonil WG is 2min.

6) suspension stability: measure according to the GB/T14825-2006 method.Result is that the suspensibility of embodiment 7-12 fluopicolide fludioxonil WG reaches more than 90%.

7) mensuration of thermodynamic stability: sample is enclosed in the ground wide-mouth bottle, stored for 4 weeks in (50 ± 1) ℃ insulating box after, analyzed embodiment 7-12 fluopicolide fludioxonil WG active constituent content.Result shows that the active ingredient resolution ratio is less than 5%.

Embodiment 13: the aqueous emulsion of composite bactericide

Preparation: 20% fluopicolide fludioxonil WP (1: 1, w/w): get the 96% former medicine 10.42kg of fluopicolide, the 96% former medicine 10.42kg of fludioxonil adds xanthans 1.2kg, NNO0.8kg, CMC1.5kg, neopelex 5kg is through mix and blend, under high-speed stirred, water 70.66kg is joined in oil phase, form the aqueous emulsion of favorable dispersibility.Quality testing meets relevant criterion.

Embodiment 14: the suspending agent of composite bactericide

20% fluopicolide fludioxonil WP (1: 1, w/w): get the 96% former medicine 10.42kg of fluopicolide, the 96% former medicine 10.42kg of fludioxonil adds NNO2kg, CMC1.5kg, neopelex 5kg, water 70.66kg take water as medium, adds active component, auxiliary agent to mix with abrasive media in the sand milling still and grinds, obtain the homogeneous suspension liquid body, liquid is separated making suspending agent with abrasive media.Quality testing meets relevant criterion.

The bactericidal effect of compound sterilizing agent formulation

Experimental example 1: the virulence test of different proportioning composite bactericides to the cucumber root rot bacterium

1.1 for the examination material

1.1.1 for trying bacterial classification: the cucumber root rot bacterium, pick up from Dingzhou City in Hebei Province Zhao Cun township Xi Gandecun, through separating pathogen identification, fusarium pine root fungus (Fusarium solani), Phomopsis pine root fungus (Phomopsis sp), rotten mould pine root fungus (Pythium myriotylum) and phytophthora root rot bacterium (Phytophthora drechsleri) are arranged.

1.1.2 for trying medium: potato dextrose agar (PDA) medium (potato 200g, glucose 20g, agar 20g, deionized water 1000mL).

1.1.3 supply the reagent product: the wetting powder of embodiment 1-6, fluopicolide and fludioxonil quality proportioning are 1～4: 1～3, prepare equally the test medicine of fluopicolide and fludioxonil quality proportioning 9～1: 1～9 with former medicine, carry out virulence test.

1.2 test method

1.2.1 colony diameter mensuration bactericide and the mixture virulence to the cucumber root rot bacterium

With the 96% former medicine of fluopicolide and the former medicinal acetone solution of 96% fludioxonil, be diluted to again the liquid of series concentration, in liquid: medium (PDA) the volume ratio ratio of 1: 9, add mixing in the medium that is cooled to 60 ℃ of left and right after dissolving, the culture dish that adds diameter 9cm, make pastille PDA dull and stereotyped, blank adds the coordinative solvent of equivalent; The cucumber root rot bacterial strain diameter of the preculture 4d card punch that is 5mm is beaten along colony edge got the bacterium cake, be inoculated into the PDA culture medium flat plate central authorities that contain series concentration, every ware 1 ferfas cake, mycelia faces down, cover the ware lid, every processing repeats 3 times, is placed in to adopt the right-angled intersection method to measure colony diameter after 25 ℃ of incubators are cultivated 4d.Calculate the inhibiting rate of each concentration liquid, utilize the DPS Software of Data Statistics to obtain virulence regression equation (Y=a+bx), EC that fluopicolide, fludioxonil and composition thereof suppress the cucumber root rot mycelial growth₅₀Value and correlation coefficient.

Inhibiting rate (%)=(the contrast bacterium colony increases diameter-processing bacterium colony and increases diameter) * 100/ (the contrast bacterium colony increases diameter)

1.2.2 the virulence synergy of fungicide compounding

Calculate the not mixed coefficient of synergism of with medicament according to Wadley (1967) formula:

${\mathrm{EC}}_{50}\left(\mathrm{th}\right)=\frac{a+b}{a/{\mathrm{EC}}_{50}\left(A\right)+b/{\mathrm{EC}}_{50}\left(B\right)}$

SR＝EC₅₀(th)/EC₅₀(ob)

In following formula, a, b represent fluopicolide (A), two kinds of bactericide of fludioxonil (B) shared ratio in mixture; EC₅₀(A) and EC₅₀(B) represent respectively the actual observation EC of A and B₅₀Value, EC₅₀(th) expression A, two kinds of bactericide of B are pressed a: the mixed theoretical EC of b₅₀Value, EC₅₀(ob) be that A, two kinds of bactericide of B are pressed a: the mixed actual observation EC of b₅₀Value.SR＞1.5, synergy; SR＜0.5, antagonism; SR represents addition between 0.5 and 1.5.

2. result of the test

As known from Table 3, fluopicolide and fludioxonil be mixed suppress to suppress cucumber root rot bacterium mycelial growth the synergy proportioning 5～1: in 1～3 scope, coefficient of synergism is greater than 1.5, have synergistic effect preferably, wherein fluopicolide and fludioxonil mass ratio are that synergy in 1: 1 o'clock is the strongest.

Table 3 fluopicolide and fludioxonil are mixed to the virulence synergy of cucumber root rot bacterium

The effect of experimental example 2: embodiment 1 composite bactericide wetting powder (WP) control cucumber downy mildew, potato/tomato late blight, cucumber/graw mold of tomato, cucumber root rot and capsicum epidemic disease

1. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the cucumber downy mildew test of pesticide effectiveness

Test according to " bactericidal agent for preventing and treating cucumber downy mildew test of pesticide effectiveness criterion " in medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion ".Susceptible variety is cucumber variety " capital is green ".The experimental field is positioned at Dingzhou City Zhao Cun township, Hebei province Xi Gandecun, breed selection, field planting on January 25 in 2012, line-spacing 1m, spacing in the rows 0.3m.

Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm², 200g a.i./hm², 250g a.i./hm²The contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage is 1031g a.i./hm²50% dimethomorph WP, dispenser dosage are 375g a.i./hm²Blank spray clear water.Every processing 4 times repeats, totally 24 residential quarters, and randomized arrangement, the residential quarter area is 18m approximately²

Begin to spray medicine before downy mildew naturally-occurring, share medicine 3 times, be 7 days interval.Spraying time is respectively on March 5th, 2012, March 12, March 19.Adopt Jacto-HD400 type manual sprayer evenly to spray medicine.Be 900L/hm with amount of liquid medicine²

Investigation morbidity in 7 days (on March 26th, 2012) after last 1 dispenser.Random 4 sampling surveys in every residential quarter, every some investigation 2 strains, the whole blades of every strain investigation, the percentage that accounts for whole blade area by lesion area on each blade is divided sick level.0 grade: without scab; 1 grade: scab accounts for below 5% of whole leaf area; 3 grades: scab accounts for 6%～10% of whole leaf area; 5 grades: scab accounts for 11%～25% of whole leaf area; 7 grades: scab accounts for 26%～50% of whole leaf area; 9 grades: scab accounts for more than 51% of whole leaf area.

Fragmentary morbidity before dispenser for the first time, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.In the data SPSS13.0 software, Fisher ' s least significant difference (LSD) is measured and is carried out statistical analysis.

Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) * 100

Disease index=∑ (the sick numbers of sheets at different levels * relative disease value of series)/(investigating total number of sheets * 9) * 100

Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] * 100

As known from Table 4,20% fluopicolide fludioxonil WP180～250g a.i./hm²Foliage-spray has good control efficiency (80.8%～91.2%) to cucumber downy mildew.Spray 20% fluopicolide fludioxonil WP250g a.i./hm²Preventive effect be 91.2%, be significantly higher than and spray 50% dimethomorph WP375g a.i./hm²Control efficiency, with 68.75% fluopicolide propamocarb SC1031g a.i./hm²Control efficiency suitable.Spray 20% fluopicolide fludioxonil WP200g a.i./hm²Preventive effect be 86.2%, and spray 50% dimethomorph WP375g a.i./hm²Preventive effect suitable.Show before cucumber downy mildew occurs or employing 20% fluopicolide fludioxonil WP180～250g a.i./hm when just occuring²Foliage-spray has control efficiency preferably to cucumber downy mildew.

The control efficiency of table 4 20% fluopicolide fludioxonil WP to cucumber downy mildew

Process	Sick leaf rate (%)	Disease index	Control efficiency (%)
				20% fluopicolide fludioxonil WP180g a.i./hm2	8.35	2.49	80.8c
20% fluopicolide fludioxonil WP200g a.i./hm2	6.17	1.80	86.2b
				20% fluopicolide fludioxonil WP250g a.i./hm2	5.10	1.15	91.2a
68.75% fluopicolide propamocarb SC1031g a.i./hm2	4.07	0.98	92.5a
				50% dimethomorph WP375g a.i./hm2	7.30	1.63	87.5b
Blank	23.09	13.10	-

Annotate: with indifference remarkable (P=0.05) between the rear identical lowercase alphabet registration certificate of columns pick.Lower same.

2. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the potato late blight test

Test according to " bactericidal agent for preventing and treating potato late blight test of pesticide effectiveness criterion " in medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion ".Local susceptible variety is Potato Cultivars " Favorita ".The experimental field is positioned at Shizigou township, Chongli County, Hebei province seed farm, plantation on April 25th, 2012, and line-spacing 0.6m, spacing in the rows 0.3m, potato late blight occurs over the years.

Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm², 200g a.i./hm², 250g a.i./hm²The contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage is 1031g a.i./hm²50% dimethomorph WP, dispenser dosage are 375g a.i./hm²Blank spray clear water.Every processing 4 times repeats, totally 24 residential quarters, randomized arrangement.Residential quarter area 20m², establish guard row.

Begin to spray medicine before field natural occurrence, share medicine 3 times, 7～10 days intervals.Spraying time is respectively June 20, June 27, July 4.Fine during dispenser.Adopt Jacto-HD400 type manual sprayer spray medicine, spraying is evenly thoughtful.Be 900L/hm with amount of liquid medicine²Whole duration of test affects without harsh weather.

Investigation in 7 days (on July 11st, 2012) incidence after last dispenser.5 sampling surveys of every residential quarter diagonal, every some investigation 2 strains, the whole compound leaves of every strain investigation, the percentage that accounts for whole compound leaf area by lesion area on each compound leaf is divided sick level.0 grade: without scab; 1 grade: scab accounts for below 5% of whole compound leaf area; 3 grades: scab accounts for 6%～10% of whole compound leaf area; 5 grades: scab accounts for 11%～25% of whole compound leaf area; 7 grades: scab accounts for 26%～50% of whole compound leaf area; 9 grades: scab accounts for more than 51% of whole compound leaf area.

Fragmentary morbidity before dispenser for the first time, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index and preventive effect.In the data SPSS13.0 software, Fisher's least significant difference (LSD) is measured and is carried out statistical analysis.

Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) * 100

Disease index=∑ (the sick numbers of sheets at different levels * relative disease value of series)/(investigating total number of sheets * 9) * 100

Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] * 100 as known from Table 5,20% fluopicolide fludioxonil WP180～250g a.i./hm²Foliage-spray has good control efficiency (83.4%～90.9%) to potato late blight.Spray 20% fluopicolide fludioxonil WP250g a.i./hm²Preventive effect be 90.9%, be significantly higher than Suo and execute 50% dimethomorph WP375g a.i./hm²Control efficiency, with 68.75% fluopicolide propamocarb SC1031g a.i./hm²Control efficiency suitable.Spray 20% fluopicolide fludioxonil WP200g a.i./hm²Preventive effect be 87.5%, and spray 50% dimethomorph WP375g a.i./hm²Preventive effect suitable.Show before potato late blight occurs or when just occuring, employing 20% fluopicolide fludioxonil WP180～250g a.i./hm²Foliage-spray has preventive effect preferably to potato late blight.

The control efficiency of table 5 20% fluopicolide fludioxonil WP to potato late blight

Process	Sick leaf rate (%)	Disease index	Control efficiency (%)
				20% fluopicolide fludioxonil WP180g a.i./hm2	9.82	2.06	83.4c
20% fluopicolide fludioxonil WP200g a.i./hm2	6.84	1.55	87.5b
				20% fluopicolide fludioxonil WP250g a.i./hm2	5.69	1.13	90.9a
68.75% fluopicolide propamocarb SC1031g a.i./hm2	4.31	1.02	91.7a
				50% dimethomorph WP375g a.i./hm2	6.36	1.43	88.4b
Blank	22.13	12.38	-

3. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the tomato late blight test of pesticide effectiveness

The experimental field is positioned at Xin Xing village, Dingzhou City Yang Jiazhuan township, Hebei province, is the breed selection tomato, and tomato variety is susceptible variety " Borrow's tower ".Plantation on February 18th, 2012, line-spacing 0.8m, spacing in the rows 0.3m.

Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm², 200g a.i./hm², 250g a.i./hm²The contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage is 1031g a.i./hm²50% dimethomorph WP, dispenser dosage are 375g a.i./hm²Blank spray clear water.Every processing 4 times repeats, totally 24 residential quarters, randomized arrangement, residential quarter area 18m²

Premorbid begins to spray medicine, shares medicine 3 times, and be 7 days interval.Spraying time is respectively on April 18th, 2012, April 25, May 2.Adopt Jacto-HD400 type manual sprayer evenly to spray medicine.Be 900L/hm with amount of liquid medicine²

Investigation in 7 days (on May 9th, 2012) incidence after last 1 dispenser.Random 5 sampling surveys in every residential quarter, every some investigation 2 strains, the whole compound leaves of every strain investigation, the percentage that accounts for whole compound leaf area by lesion area on each compound leaf is divided sick level.0 grade: without scab; 1 grade: scab accounts for below 5% of whole compound leaf area; 3 grades: scab accounts for 6%～10% of whole compound leaf area; 5 grades: scab accounts for 11%～25% of whole compound leaf area; 7 grades: scab accounts for 26%～50% of whole compound leaf area; 9 grades: scab accounts for more than 51% of whole compound leaf area.

Fragmentary morbidity before dispenser for the first time, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.In the data SPSS13.0 software, Fisher's least significant difference (LSD) is measured and is carried out statistical analysis.

Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) * 100

Disease index=∑ (the sick numbers of sheets at different levels * relative disease value of series)/(investigating total number of sheets * 9) * 100

Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] * 100

As known from Table 6,20% fluopicolide fludioxonil WP180～250g a.i./hm²Foliage-spray has good control efficiency (82.0%～91.3%) to tomato late blight.Spray 20% fluopicolide fludioxonil WP250 g a.i./hm²Preventive effect be 91.3%, be significantly higher than and spray 50% dimethomorph WP375g a.i./hm²Control efficiency, with 68.75% fluopicolide propamocarb SC1031g a.i./hm²Control efficiency suitable.Spray 20% fluopicolide fludioxonil WP200g a.i./hm²Preventive effect be 87.6%, and spray 50% dimethomorph WP375g a.i./hm²Preventive effect suitable.Show before tomato late blight occurs or when just occuring, employing 20% fluopicolide fludioxonil WP180～250g a.i./hm²Foliage-spray has preventive effect preferably to tomato late blight.

The control efficiency of table 6 20% fluopicolide fludioxonil WP to tomato late blight

Process	Sick leaf rate (%)	Disease index	Control efficiency (%)
				20% fluopicolide fludioxonil WP180g a.i./hm2	8.69	1.72	82.0c
20% fluopicolide fludioxonil WP200g a.i./hm2	5.86	1.21	87.6b
				20% fluopicolide fludioxonil WP250g a.i./hm2	4.52	0.82	91.3a
68.75% fluopicolide propamocarb SC1031g a.i./hm2	3.72	0.69	92.7a
				50% dimethomorph WP375g a.i./hm2	5.77	1.18	87.3b
Blank	17.73	9.54	-

4. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the capsicum epidemic disease test of pesticide effectiveness

In medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion ", " bactericidal agent for preventing and treating capsicum epidemic disease test of pesticide effectiveness criterion " carries out.Susceptible variety is capsicum variety " the ears of an ox or cow king ".The experimental field is positioned at the solid cities and towns Bei Zhuantoucun in Dingxing County, Hebei province, is the breed selection capsicum, line-spacing 0.8m, spacing in the rows 0.3m.

Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm², 200g a.i./hm², 250g a.i./hm²The contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage is 1031g a.i./hm²50% dimethomorph WP, dispenser dosage are 375g a.i./hm²Blank spray clear water.Every processing 4 times repeats, totally 24 residential quarters, randomized arrangement, residential quarter area 24m²

Premorbid begins to spray medicine, shares medicine 3 times, and be 7 days interval.Spraying time is respectively on May 4th, 2012, May 11, May 18.Adopt evenly thoughtful spray medicine of Jacto-HD400 type manual sprayer.Be 900L/hm with amount of liquid medicine²

After last 1 dispenser, investigate 7 days (on May 25th, 2012).Random 5 sampling surveys in every residential quarter, every some investigation 10 strains.Stage division: 0 grade: anosis; 1 grade: rhizome section is without scab, indivedual branches or fruit morbidity; 3 grades: the slight morbidity of rhizome section or the following branch of half or fruit morbidity; 5 grades: rhizome section's moderate morbidity or branch more than half or fruit morbidity; 7 grades: rhizome section seriously falls ill, and large of plant is wilting; 9 grades: the whole wilting death of plant.Fragmentary morbidity before dispenser for the first time, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.In the data SPSS13.0 software, Fisher's least significant difference (LSD) is measured and is carried out statistical analysis.

Diseased plant rate (%)=(the total strain number of diseased plant number/investigation) * 100

Disease index=∑ (diseased plant numbers at different levels * relative disease value of series)/(investigating total strain number * 9) * 100

Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] * 100

As known from Table 7,20% fluopicolide fludioxonil WP180～250g a.i./hm²Foliage-spray has good control efficiency (86.1%～91.0%) to capsicum epidemic disease.Spray 20% fluopicolide fludioxonil WP250g a.i./hm²Preventive effect be 91.0%, be significantly higher than and spray 50% dimethomorph WP375g a.i./hm²Control efficiency, with 68.75% fluopicolide propamocarb SC1031g a.i./hm²Control efficiency suitable.Spray 20% fluopicolide fludioxonil WP180～200g a.i./hm²Preventive effect be 86.1%～87.8%, and spray 50% dimethomorph WP375g a.i./hm²Preventive effect suitable.Show before capsicum epidemic disease occurs or employing 20% fluopicolide fludioxonil WP180～250g a.i./hm when just occuring²Foliage-spray has control efficiency preferably to capsicum epidemic disease.

The control efficiency of table 7 20% fluopicolide fludioxonil WP to capsicum epidemic disease

Process	Diseased plant rate (%)	Disease index	Control efficiency (%)
				20% fluopicolide fludioxonil WP180g a.i./hm2	13.50	2.06	86.1b
20% fluopicolide fludioxonil WP200g a.i./hm2	11.00	1.78	87.8b
				20% fluopicolide fludioxonil WP250g a.i./hm2	7.00	1.33	91.0a
68.75% fluopicolide propamocarb SC1031g a.i./hm2	7.50	1.39	90.5a
				50% dimethomorph WP375g a.i./hm2	9.50	2.06	86.1b
Blank	25.00	15.00	-

5. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the gray mold of cucumber test of pesticide effectiveness

Test according to the GB/T17980.28-2000 of medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion " " bactericidal agent for preventing and treating eliminating vegetable botrytis test of pesticide effectiveness criterion ".Cucumber variety is conventional variety " De Ruite 787 ".The experimental field is positioned at Dingzhou City Zhao Cun township, Hebei province Xi Gandecun, field planting on February 14 in 2012.

Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm², 200g a.i./hm², 250g a.i./hm²The contrast medicament: 50% Boscalid WG, dispenser dosage are 300g a.i./hm²50% fludioxonil WP, dispenser dosage are 100g a.i./hm²Blank spray clear water.Every processing 4 times repeats, totally 24 residential quarters, randomized arrangement, residential quarter area 18m²

Premorbid begins to spray medicine, shares medicine 3 times, and be 7 days interval.Spraying time is respectively on March 6th, 2012, March 13, March 20.Adopt evenly thoughtful spray medicine of Jacto-HD400 type manual sprayer.Be 900L/hm with amount of liquid medicine²

After last 1 dispenser, investigate 7 days (on March 27th, 2012).The investigation incidence, every residential quarter is got 5 points at random, and whole fruits of every some investigation 2 strains record the sick fruit number that gray mold causes.Gray mold of cucumber is take fruit as unit survey: 0 grade: without scab; 1 grade: residual flower morbidity; 3 grades: the morbidity of areola section; 5 grades: scab length accounts for below 10% of fruit; 7 grades: scab length accounts for 11%～25% of fruit; 9 grades: scab length accounts for more than 26% of fruit.Calculate diseased fruit rate, disease index and control efficiency according to above stage division, in the data SPSS13.0 software, Fisher's least significant difference (LSD) is measured and is carried out statistical analysis.

Diseased fruit rate (%)=(the total fruit of sick fruit number/investigation number) * 100

Disease index=∑ (sick fruit number * relative disease value of series at different levels)/(the total fruit of investigation number * 9) * 100

Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] * 100

As known from Table 8,20% fluopicolide fludioxonil WP180～250g a.i./hm²Foliage-spray has good control efficiency (80.1%～89.1%) to gray mold of cucumber.Spray 20% fluopicolide fludioxonil WP250g a.i./hm²Preventive effect be 89.1%, and spray 50% Boscalid WG300g a.i./hm²Control efficiency suitable, be significantly higher than and spray 50% fludioxonil WP100g a.i./hm²Control efficiency.Spray 20% fluopicolide fludioxonil WP200g a.i./hm²Preventive effect be 86.6%, and spray 50% fludioxonil WP100g a.i./hm²Control efficiency suitable.Show gray mold of cucumber occur before or when just occuring, adopt 20% fluopicolide fludioxonil WP180～250g a.i./hm²Carry out foliage-spray gray mold of cucumber is had preventive effect preferably.

The control efficiency of table 8 20% fluopicolide fludioxonil WP to gray mold of cucumber

Process	Epilepsy fruit rate (%)	Disease index	Control efficiency (%)
				20% fluopicolide fludioxonil WP180g a.i./hm2	36.71	7.04	80.1c
20% fluopicolide fludioxonil WP200g a.i./hm2	28.81	4.79	86.6b
				20% fluopicolide fludioxonil WP250g a.i./hm2	22.37	3.89	89.1a
50% Boscalid WG300g a.i./hm2	19.87	3.70	89.5a
				50% fludioxonil WP100g a.i./hm2	26.79	4.79	86.4b
Blank	68.75	35.55	-

6. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the graw mold of tomato test of pesticide effectiveness

According to the GB/T17980.28-2000 of medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion " " bactericidal agent for preventing and treating eliminating vegetable botrytis test of pesticide effectiveness criterion ' ' test.Tomato variety is conventional variety " east victory hooligan ".The experimental field is positioned at Tian Cunpu village, high woods villages and small towns, Xushui County, Hebei province, field planting on February 10 in 2012.

Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm², 200g a.i./hm², 250g a.i./hm²The contrast medicament: 50% Boscalid WG, dispenser dosage are 300g a.i./hm²50% fludioxonil WP, dispenser dosage are 100g a.i./hm²Blank spray clear water.Every processing 4 times repeats, totally 24 residential quarters, randomized arrangement, residential quarter area 18m²

Premorbid begins to spray medicine, shares medicine 3 times, and be 7 days interval.Spraying time is respectively on March 4th, 2012, March 11, March 18.Adopt evenly thoughtful spray medicine of Jacto-HD400 type manual sprayer.Be 900L/hm with amount of liquid medicine²

After last 1 dispenser, investigate 7 days (on March 25th, 2012).The investigation incidence, every residential quarter is got 5 points at random, and whole fruits of every some investigation 2 strains record the sick fruit number that gray mold causes.Gray mold fruit grade scale is: 0 grade: without scab; 1 grade: residual petal morbidity or column cap morbidity; 3 grades: sepal rots or the column cap morbidity spreads to areola section; 5 grades: there is the spot of infiltration in areola section but without mould layer; 7 grades: there is mould layer in areola section but does not expand to other position; 9 grades: the mould layer of areola section expands to other position.Calculate diseased fruit rate, disease index and control efficiency according to above stage division, in the data SPSS13.0 software, Fisher's least significant difference (LSD) is measured and is carried out statistical analysis.

Diseased fruit rate (%)=(the total fruit of sick fruit number/investigation number) * 100

Disease index=∑ (sick fruit number * relative disease value of series at different levels)/(the total fruit of investigation number * 9) * 100

Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] * 100

As known from Table 9,20% fluopicolide fludioxonil WP150～300g a.i./hm²Foliage-spray has good control efficiency (81.4%～91.8%) to graw mold of tomato.Spray 20% fluopicolide fludioxonil WP300g a.i./hm²Preventive effect be 91.8%, and spray 50% Boscalid WG300g a.i./hm²Control efficiency suitable, be significantly higher than and spray 50% fludioxonil WP100g a.i./hm²Control efficiency.Spray 20% fluopicolide fludioxonil WP200g a.i./hm²Preventive effect be 88.0%, and spray 50% fludioxonil WP100g a.i./hm²Control efficiency suitable.Show graw mold of tomato occur before or adopt 20% fluopicolide fludioxonil WP150～300g a.i./hm when just occuring²Carry out foliage-spray graw mold of tomato is had control efficiency preferably.

The control efficiency of table 9 20% fluopicolide fludioxonil WP to graw mold of tomato

Process	Diseased fruit rate (%)	Disease index	Control efficiency (%)
				20% fluopicolide fludioxonil WP180g a.i./hm2	24.97	4.83	81.4c
20% fluopicolide fludioxonil WP200g a.i./hm2	18.92	3.15	88.0b
				20% fluopicolide fludioxonil WP250g a.i./hm2	14.16	2.09	91.8a
50% Boscalid WG300g a.i./hm2	12.39	2.07	91.9a
				50% fludioxonil WP100g a.i./hm2	17.54	3.00	88.2b

Blank

50.98

26.44

-

7. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the cucumber root rot test of pesticide effectiveness

The experimental field is positioned at Dingzhou City Zhao Cun township, Hebei province Xi Gandecun, and cucumber variety is conventional variety " De Ruite 787 ", field planting on February 14 in 2012, line-spacing 1m, spacing in the rows 0.3m.

Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 1000 times of liquid, 900 times of liquid, 700 times of liquid.The contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage are 600 times of liquid; 50% fludioxonil WP, dispenser dosage are 4500 times of liquid, and blank is filled with clear water.Every processing 4 times repeats, totally 24 residential quarters, randomized arrangement, residential quarter area 24m²

Before cucumber root rot occurs, every strain perfusion liquid 250mL.A situation arises respectively to process root rot every the 15d investigation after medicine, records the diseased plant number, calculates control efficiency according to diseased plant rate.In the data SPSS13.0 software, Fisher's least significant difference (LSD) is measured and is carried out statistical analysis.

As known from Table 10,20% fluopicolide fludioxonil WP1000～700 times of liquid irrigating roots have good control efficiency (49.1%～63.7%) to cucumber root rot, are significantly higher than the control efficiency of SC600 times of liquid of 68.75% fluopicolide propamocarb and 50% fludioxonil WP4500 times liquid.Show and adopt 20% fluopicolide fludioxonil WP700～1000 times of liquid irrigating roots to have control efficiency preferably to cucumber root rot before cucumber root rot occurs.

The control efficiency of table 10 20% fluopicolide fludioxonil WP to cucumber root rot

Process	Epilepsy strain rate (%)	Control efficiency (%)
			20% WP1000 times of fluopicolide fludioxonil liquid	15.50	49.1c
20% WP900 times of fluopicolide fludioxonil liquid	12.50	59.1b
			20% WP700 times of fluopicolide fludioxonil liquid	11.00	63.7a
68.75% SC600 times of fluopicolide propamocarb liquid	19.50	36.1d
			50% fludioxonil WP4500 times liquid	20.50	32.8d
Blank	30.50	-

Field trial shows: 20% fluopicolide fludioxonil WP180～250g a.i./hm²Foliar spray all has good preventive and therapeutic effect to cucumber downy mildew, potato/tomato late blight, cucumber/graw mold of tomato and capsicum epidemic disease, 20% fluopicolide fludioxonil WP700～1000 times of liquid irrigating roots have good control efficiency to cucumber root rot, can use in the field produces.

Composite bactericide fluopicolide fludioxonil control cucumber downy mildew in experimental example 3: embodiment 7,13,14

The experimental field is positioned at Dingzhou City Zhao Cun township, Hebei province Xi Gandecun, and cucumber variety is conventional variety " De Ruite 787 ", field planting on February 14 in 2012, line-spacing 1m, spacing in the rows 0.3m.

Dispenser dosage is 180g a.i./hm², 200g a.i./hm², 250g a.i./hm²Result shows, the fluopicolide fludioxonil WP180 in embodiment 7,13,14～250g a.i./hm²Foliage-spray has good control efficiency (81.4%～92.6%) to cucumber downy mildew.

The control efficiency of fluopicolide fludioxonil in table 15 embodiment 7,13,14 to cucumber downy mildew

Claims (9)

Translated fromChinese

1.一种复配杀菌剂，由氟吡菌胺和咯菌腈组成；其中，所述氟吡菌胺与咯菌腈的质量比为1～9∶1～90。1. A compound bactericide, consisting of fluopicolide and fludioxonil; wherein, the mass ratio of fluopicolide to fludioxonil is 1-9:1-90.2.根据权利要求1所述的复配杀菌剂，其特征在于：所述氟吡菌胺与咯菌腈的质量比为1～7∶1～5。2. The compound fungicide according to claim 1, characterized in that: the mass ratio of fluopyram to fludioxonil is 1-7:1-5.3.根据权利要求1或2所述的复配杀菌剂，其特征在于：所述氟吡菌胺和咯菌腈的质量比为1～5∶1～3。3. The compound fungicide according to claim 1 or 2, characterized in that: the mass ratio of fluopyram to fludioxonil is 1-5:1-3.4.一种复配杀菌剂制剂，由权利要求1-3中任一项所述的复配杀菌剂和农药制剂上可接受的助剂组成。4. A compound fungicide preparation, consisting of the compound fungicide described in any one of claims 1-3 and an acceptable auxiliary agent on the pesticide preparation.5.根据权利要求4所述的复配杀菌剂制剂，其特征在于：所述复配杀菌剂制剂的剂型选自下述至少一种：可湿性粉剂、水分散粒剂、可溶性粉剂、可溶性粒剂、水剂、可溶性液剂、乳油、水乳剂、微乳剂和悬浮剂。5. The compound fungicide preparation according to claim 4, characterized in that: the dosage form of the compound fungicide preparation is selected from at least one of the following: wettable powder, water dispersible granule, soluble powder, soluble granule formulations, aqueous solutions, soluble solutions, emulsifiable concentrates, emulsions in water, microemulsions and suspensions.6.根据权利要求4所述的复配杀菌剂制剂，其特征在于：所述助剂包括下述至少一种：分散剂、润湿剂、粘结剂、增稠剂、抗泡剂、崩解剂和填料。6. The compound fungicide preparation according to claim 4, characterized in that: the auxiliary agent comprises at least one of the following: dispersant, wetting agent, binder, thickener, antifoaming agent, disintegrant Debonding agents and fillers.7.权利要求1-3中任一项所述的复配杀菌剂或权利要求4-6中任一项所述的复配杀菌剂制剂在防治植物病害中的应用。7. Application of the compound fungicide described in any one of claims 1-3 or the compound fungicide preparation described in any one of claims 4-6 in the prevention and treatment of plant diseases.8.根据权利要求7所述的复配杀菌剂或复配杀菌剂制剂，其特征在于：所述植物为瓜果和/或蔬菜；所述病害包括：卵菌病害、灰霉病和根腐病。8. The compound fungicide or compound fungicide preparation according to claim 7, characterized in that: the plants are fruits and/or vegetables; the diseases include: oomycete disease, gray mold and root rot sick.9.根据权利要求8所述的复配杀菌剂或复配杀菌剂制剂，其特征在于：所述植物病害选自下述至少一种：黄瓜霜霉病、马铃薯晚疫病、番茄晚疫病、黄瓜灰霉病、番茄灰霉病、辣椒疫病和黄瓜根腐病。9. The compound fungicide or compound fungicide preparation according to claim 8, characterized in that: the plant disease is selected from at least one of the following: cucumber downy mildew, potato late blight, tomato late blight, cucumber Botrytis cinerea, tomato botrytis, pepper blight and cucumber root rot.