CN103062479A - Magnetic responsive micro valve based on micro-fluidic chip and production method thereof - Google Patents

Abstract

The invention relates to a magnetic responsive micro valve based on a micro-fluidic chip and a production method thereof. Microstructures and micro-channels are arranged on the surface of the micro-fluidic chip, a hydrophilic/dehydrophilic gate controlled switch is arranged on a main micro-channel, the surfaces of the micro-channels are modified through magnetic responsive molecules, reversible changes of surface appearance and chemical construction of the micro-channels cause changes of surface invasion under stimulation of an outer magnetic field, movement of micro-fluid on the surfaces of the micro-channels is controlled, and therefore the intelligent micro valve achieves control on micro-fluid flowing. The magnetic responsive micro valve is mainly applied to the related fields such as electrophoretic separation, chromatographic separation and immunoassay, achieves intelligent driving and control on micro-fluid, greatly reduces micro-fluid driving and control cost, has the advantages of being portable, economical, fast and efficient, and is a novel analysis technique for micro-fluid driving and control.

Description

A kind of little valve of magnetic response based on micro-fluidic chip and preparation method thereof

Technical field

The present invention relates to a kind of little valve of magnetic response based on micro-fluidic chip and preparation method thereof, there are microstructure and micro passage in this micro-fluidic chip surface, main micro passage is provided with the hydrophilic/hydrophobic door controlled switch, by the magnetic responsiveness molecule microchannel surface is carried out modification, under external electrical field stimulation, the surface topography of micro passage and the reversibility of chemical structure change the variation that causes surface wettability, control liquid fluid is in the motion of microchannel surface, thereby realize intelligent little valve of the control of microfluidic flow is mainly used in electrophoretic separation, chromatographic isolation, the association areas such as immunoassay.

Background technique

Microfluidic analysis chip has microminiaturization, automation, the advantage such as integrated, convenient and quick as a kind of novel analysis platform; obtain a wide range of applications for example synthetic screening of cell biology, analytical chemistry, environmental monitoring and protection, judicial expertise and medicine in a lot of fields.In microfluidic analysis chip, the accurate sample introduction of Micro-volume liquid is the key of Sample handling and analysis, just need such operation in for example micro-fluid control chip electrophoretic separation, chromatographic isolation, the immunoassay, this is because the characteristics of microfluidic analysis chip will operate the microfluid under the micro-scale and control, and it is extremely small as the Fluid Volume of operation and control object, cause flow characteristic and the macroscopic view of microfluid to be very different, negligible phenomenon becomes the major influence factors of Fluid Flow in A under micro-scale under macro-scale.In recent years, on microfluidic analysis chip, how to realize driving and control to microfluid, become a research difficult problem and focus in the microfluidic analysis chip technology.

Driving and control to microfluid in the micro-fluidic chip can realize by little valve.In recent years, along with development and the maturation of microflow control technique, research and develop a lot of methods and device, realized to a certain extent driving and control to microfluid in the micro-fluidic chip, also had some narrow limitation simultaneously.Aspect little valve research, according to whether dynamic driving mechanism is divided into active and passive valves, the little valve of active valve such as electromagnetism, the little valve of static, the little valve of marmem, piezoelectric micromotor valve, hot pneumatic micro valve etc., the little valve of this class can be realized the ON/OFF of valve, braking ability is good, seal is high, but that shortcoming is complex structure, volume is large, be difficult to realize integrated on the chip.Passive valves does not need the external impetus braking, relies on the pressure difference of valve both sides to realize switch, its small volume, but can not initiatively carry out ON/OFF or the switching of valve.

Yet, routine techniques is difficult to finish intelligent drives and the control to microfluid in the micro passage, therefore, thereby by utilize external magnetic field to come the infiltrating variation of control surface to drive and the control micro-fluidic chip in microfluid, it is a kind of convenient, quick, efficiently, microfluid drives and control technique cheaply to develop, should be one of research direction of little valve on the micro-fluidic chip, substantial breakthrough is not yet arranged at present.

Summary of the invention

The purpose of this invention is to provide a kind of little valve of magnetic response based on micro-fluidic chip and preparation method thereof, there are microstructure and micro passage in this micro-fluidic chip surface, main micro passage is provided with the hydrophilic/hydrophobic door controlled switch, by the electrical response performance molecule microchannel surface is carried out modification, under external magnetic stimulation, the surface topography of micro passage and the reversibility of chemical structure change the variation that causes surface wettability, control liquid fluid is in the motion of microchannel surface, thereby realize intelligent little valve of the control of microfluidic flow is mainly used in electrophoretic separation, chromatographic isolation, the association areas such as immunoassay.

For achieving the above object, the present invention adopts following operating procedure:

(1) microstructure and the micro passage figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.

(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane microstructure and micro passage, comprise sample holes, separate the main passage and separate subchannel.

(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.

(4) carry out the magnetic responsiveness molecule in the micro passage to the surface modification of micro passage.

(5) apply external magnetic field, the wellability of microchannel surface is from hydrophilic to hydrophobic adjustable.

Among the present invention, can be PMMA, PC, PVC, COC, copper, aluminium, stainless steel, silicon chip, glass wafer based on the chip substrates of the little valve of magnetic responsiveness of micro-fluidic chip, also commercially available all kinds of common CD CD.

Among the present invention, can be by numerical control mill quarter, laser ablation, LIGA technology, method of molding, hot-pressing method, chemical corrosion preparation, also available soft lithographic technique preparation based on the microstructure of the micro-fluidic chip of the little valve of magnetic responsiveness of micro-fluidic chip and adhesive membrane and micro passage.

Among the present invention, be comprised of layers of chips based on the micro-fluidic chip of the little valve of magnetic responsiveness of micro-fluidic chip, fit with adhesive membrane between each layer chip, adhesive membrane can be that double-deck power causes adhesive membrane, also common two sided plaster film.

Among the present invention, carry out Finishing based on the micro passage of the micro-fluidic chip of the little valve of magnetic responsiveness of micro-fluidic chip with the magnetic responsiveness molecule.

Among the present invention, under the illumination of outfield, produce hydrophilic and variation hydrophobic performance based on the magnetic responsiveness molecule of the micro-fluidic chip of the little valve of magnetic responsiveness of micro-fluidic chip.

The present invention propose based on little valve of magnetic responsiveness of micro-fluidic chip and preparation method thereof, intelligent drives and control simple to operate, as to have realized microfluid, greatly reduced the cost that microfluid drives and controls, have portable, economic, characteristics fast and efficiently, in the association areas such as electrophoretic separation, chromatographic isolation, immunoassay, have a good application prospect.

Description of drawings

Fig. 1. based on the structural representation of the micro-fluidic chip of the little valve of magnetic responsiveness of micro-fluidic chip.

A. solution entrance, b. microfluidic control passage (little valve), the hydrophilic modified regions of c. magnetic response, d. magnetic response hydrophobic modification zone, e. taphole.

Specific embodiments

Embodiment 1

Microstructure and the micro passage figure of the layers of chips of the micro-fluidic chip that Computer-aided design Software for Design and drafting microfluidic flow are controlled.Utilize microstructure and the micro passage of numerical control CNC system processing preparation two-layer polymethylmethacrylate (PMMA) chip, clean each layer chip with tap water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the two sided plaster film, with required microstructure and the micro passage of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the intelligent little valve based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip, solution enters in the micro passage under the driving of external peristaltic pump, little valve zone in the micro passage, the magnetic response molecule of microchannel surface responds the outfield electric field, obtain hydrophilic ON/OFF and hydrophobic open/close switching, realized that at last little valve is to the intelligent control of microfluidic flow.

Embodiment 2

Microstructure and the micro passage figure of the layers of chips of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.Utilize microstructure and the micro passage of numerical control CNC system processing preparation two-layer disc-shaped polycarbonate (PC) (PC) chip, clean each layer chip with tap water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the two sided plaster film, with required microstructure and the micro passage of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the intelligent little valve based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip, solution enters in the micro passage under the driving of external peristaltic pump, little valve zone in the micro passage, the magnetic response molecule of microchannel surface responds the outfield electric field, obtain hydrophilic ON/OFF and hydrophobic open/close switching, realized that at last little valve is to the intelligent control of microfluidic flow.

Claims (10)

1. one kind based on little valve of magnetic responsiveness of micro-fluidic chip and preparation method thereof, there are microstructure and micro passage in this micro-fluidic chip surface, main micro passage is provided with the hydrophilic/hydrophobic door controlled switch, by the magnetic responsiveness molecule microchannel surface is carried out modification, under external electrical field stimulation, the surface topography of micro passage and the reversibility of chemical structure change the variation that causes surface wettability, and control liquid fluid is in the motion of microchannel surface, thereby realization is to intelligent little valve of the control of microfluidic flow.

2. by little valve of magnetic responsiveness based on micro-fluidic chip claimed in claim 1 and preparation method thereof, it is characterized in that its making step is as follows:

(1) microstructure and the micro passage figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.

(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane microstructure and micro passage, comprise sample holes, separate the main passage and separate subchannel.

(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.

(4) carry out the magnetic responsiveness molecule in the micro passage to the surface modification of micro passage.

(5) apply external magnetic field, the wellability of microchannel surface is from hydrophilic to hydrophobic adjustable.

3. by claim 1 or 2 described little valves of magnetic responsiveness based on micro-fluidic chip and preparation method thereof, it is characterized in that, this base is micro-fluidic chip based on the Core Feature device of the little valve of magnetic responsiveness of micro-fluidic chip, and this chip can be produced in batches, repeatedly utilization, flexible design and assembling.

4. by claim 1 or 2 described little valves of magnetic responsiveness based on micro-fluidic chip and preparation method thereof, it is characterized in that, the microstructure of this little valve of magnetic responsiveness based on micro-fluidic chip and micro passage be micro-processing method by numerical control mill quarter, laser ablation, LIGA technology, method of molding, hot-pressing method, chemical corrosion, soft lithographic technique in the preparation of chip substrates surface, size is in micron level.

5. by claim 1 or 2 described little valves of magnetic responsiveness based on micro-fluidic chip and preparation method thereof, it is characterized in that, the little valve of this magnetic responsiveness based on micro-fluidic chip is to be formed by stacking by layers of chips, consists of microstructure and the micro passage network of 3 D stereo.

6. by claim 1 or 2 described little valves of magnetic responsiveness based on micro-fluidic chip and preparation method thereof, it is characterized in that, the little valve of this magnetic responsiveness based on micro-fluidic chip can be made many group microstructures and micro passage at chip piece, consist of many group control units, the flow direction of alternative control microfluid.

7. by claim 1 or 2 described little valves of magnetic responsiveness based on micro-fluidic chip and preparation method thereof, it is characterized in that the little valve of this magnetic responsiveness based on micro-fluidic chip has carried out the Finishing of electric field response molecule in microchannel surface.

8. by claim 1 or 2 described little valves of magnetic responsiveness based on micro-fluidic chip and preparation method thereof, it is characterized in that, the little valve of this magnetic responsiveness based on micro-fluidic chip carries out hydrophilic ON/OFF and the regulation and control of hydrophobic ON/OFF by the automatic identification of optical Response molecule in microchannel surface.

9. by claim 1 or 2 described little valves of magnetic responsiveness based on micro-fluidic chip and preparation method thereof, it is characterized in that the little valve of this magnetic responsiveness based on micro-fluidic chip can be realized the ON/OFF to solution fast.

10. by claim 1 or 2 described little valves of magnetic responsiveness based on micro-fluidic chip and preparation method thereof, it is characterized in that, it is portable, economical, quick, efficient that the little valve of this magnetic responsiveness based on micro-fluidic chip has, and is with a wide range of applications in electrophoretic separation, chromatographic isolation, the related numerous association areas of immunoassay.

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