CN103044128A - Preparation method and application of microbial fertilizer granules - Google Patents

Abstract

The invention relates to a preparation method and an application of microbial fertilizer granules. The invention relates to a bacillus subtilis PGPR-802 (CGMCC No.6048). Bacillus subtilis liquid is produced by adopting a liquid fermentation technology; and after the bacterial liquid, humic acid and active sludge are mixed in a mass ratio of 1: 4: 5, the microbial fertilizer granules are produced by adopting a disc granulating process. Cell experiments show that when the granules are basally applied or dressed to the soil of tomato or apple roots, the yield increase rates reach 10.4 percent and 7.3 percent respectively.

Description

A kind of preparation method of microbial fertilizer granule and application

Technical field

The present invention is by subtilis liquid depth fermentation technique, produces fermentation of bacillus subtilis bacterium liquid, mixes simultaneously humic acids and active sludge granulation, forms to have bioactive microbial fertilizer.

Background technology

Microbial fertilizer passes through the wherein vital movement of contained microorganism, increased the supply of plant elements, and by bacterium from plant materials or root soil around the breeding, improve the micro-ecological environment of plant, can reach production-increasing function, pathogenic effects that again can some pathogenic micro-organism of antagonism is by providing nutrition and alleviating the effect that crop pest causes output to increase.

The subtilis PGPR-802 that the present invention relates to is preserved in No. 3 China Committee for Culture Collection of Microorganisms common micro-organisms centers, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City on April 24th, 2012, deposit number CGMCC No. 6048, and Classification And Nomenclature isBacillus subtilisPGPR-802 is a kind of high-salt tolerance, aerobic and facultative aerobic, the bacterium that heat, pH and salt concn is had extensive diversity existence adaptive faculty, in the fermenting process of Bacillus subtillis, can absorb multiple inorganic elements, make plant become possibility by micro-biointegration, Bacillus subtillis application function on plant is comprehensively superior in addition, can strong plant, reduce pesticide residue, ecological, environmental protective improves food safety, can also produce bacteriostatic peptide and lactic acid, suppresses and alleviates vegetative bacteria and fungal disease.The effective bacterium of Bacillus subtillis is the brood cell, long quality-guarantee period, and commodity is good, can be for a long time in market circulation.

For many years, the processing of microbial fertilizer all is the by product that adopts other industry such as veterinary drug, zymin, glutamic acid fermentation industry, most processing modes only adopt the finished product microbial inoculum to mix with filler, lack fermentating metabolism product, other drying course excess Temperature, the microorganism loss is serious, has had a strong impact on the fertilizer efficiency of microbial fertilizer product, production technique of the present invention fundamentally solves these problems, and the field fertilizer efficiency of this microbial fertilizer is greatly improved.

Summary of the invention

The activation of Bacillus subtilus bacterial classification

To be kept at-20 ℃ of bacterial classification inoculations in the refrigerator to beef-protein medium in vitro, 37 ℃ of constant temperature culture 24h;

The preparation of first order seed

Preparation beef extract-peptone shake-flask culture base, with the packing of 500mL triangular flask, liquid amount 200mL, the Bacillus subtilus behind the autoclaving after the access activation, 37 ℃, 220r/min cultivates 24h;

The preparation of secondary seed

Preparation seed tank culture base, the substratum combination is as follows: yeast powder 0.3%, peptone 1%, sodium-chlor 0.5%, glucose 1%.The seed culture medium that shaking table is cultivated accesses 500L seeding tank, 37 ℃, 180r/min, ventilation ratio 1 in 5% ratio:1.5 cultivate 24h;

The cultivation of fermentor tank

The fermentation tank culture medium formula rate is as follows: peptone 0.5%, and yeast powder 0.5%, glucose 2%, corn steep liquor 1%, NaCl 0.2%, transfers 7.5,35 ℃ of pH, 200r/min, ventilation ratio 1:1 is cultured to till the generation brood cell;

Bacteria containing amount detects

Adopt dilution-plate method to detect the bacteria containing amount that fermentation is finished;

Raw material is mixed

With humic acids and the active sludge ratio mixing and stirring in 4:5;

Disc granulation

With granulating disc granulation on the material that mixes, 10% zymocyte liquid bonded pelleting is added in simultaneously spraying;

Oven drying at low temperature

Adopt steam heating cylinder chuck to 70 ℃, by induced draft fan steam is taken out of, below the oven dry moisture to 20%.

Check

Product sampling after the oven dry detects the indexs such as effective bacterium number, miscellaneous bacteria rate, moisture by the requirement of GB GB20287-2006.

Packing

Become the 20-40kg/ bag to be finished product the packing of product that is up to the standards.

Application example

Application example 1 is year August in April, 2011-2011, my unit entrusts Shandong Province's Qingzhou City soil and fertilizer to stand in tomato planting district, Bei Koubu village, Qingzhou City He Guan town, take tomato as having carried out plot experiment for studying thing, test minute three processing, each processes triplicate, every residential quarter cultivated area 30m², process 1 and execute the present invention for base and add and topdress, to process 2 bases and execute deactivation matrix and add and topdress, processing 3 is the contrast of topdressing.

The experimental result demonstration, the present invention processes one than contrast volume increase 368.9kg/ mu tomato, increases production 10.4%.

Table 1 is used the present invention and is fertilizer efficiency plot experiment result tomato

Application example 2 is year September in April, 2011-2011, my unit entrusts in Kou Bu village, Qingzhou City He Guan town, carried out plot experiment take apple as processing object, test is divided into three processing, each processes triplicate, and 10 of apple trees are processed in every residential quarter, process 1 conventional fertilizer application and add the present invention, process 2 for conventional fertilizer application adds deactivation matrix, process 3 conventional fertilizer applications.

The result shows: use obvious effect of increasing production of the present invention, process one than control group volume increase 368.9kg/ mu, stimulation ratio 7.3%.

Table 2 is used the present invention and is fertilizer efficiency plot experiment result at apple

[0001]

Sequence table

＜110〉the blue or green agricultural science and technology in capital, Shandong company limited

＜120〉a kind of preparation method of microbial fertilizer granule and application

<160> 1

<170> PatentIn version 3.5

<210> 1

<211> 1428

<212> RNA

＜213〉subtilis PGPR-802 16sRNA

<400> 1

cttcggcggc tggctcctaa aggttacctc accgacttcg ggtgttacaa actctcgtgg 60

tgtgacgggc ggtgtgtaca aggcccggga acgtattcac cgcggcatgc tgatccgcga 120

ttactagcga ttccagcttc acgcagtcga gttgcagact gcgatccgaa ctgagaacag 180

atttgtggga ttggcttaac ctcgcggttt cgctgccctt tgttctgtcc attgtagcac 240

gtgtgtagcc caggtcataa ggggcatgat gatttgacgt catccccacc ttcctccggt 300

ttgtcaccgg cagtcacctt agagtgccca actgaatgct ggcaactaag atcaagggtt 360

gcgctcgttg cgggacttaa cccaacatct cacgacacga gctgacgaca accatgcacc 420

acctgtcact ctgcccccga aggggacgtc ctatctctag gattgtcaga ggatgtcaag 480

acctggtaag gttcttcgcg ttgcttcgaa ttaaaccaca tgctccaccg cttgtgcggg 540

cccccgtcaa ttcctttgag tttcagtctt gcgaccgtac tccccaggcg gagtgcttaa 600

tgcgttagct gcagcactaa ggggcggaaa ccccctaaca cttagcactc atcgtttacg 660

gcgtggacta ccagggtatc taatcctgtt cgctccccac gctttcgctc ctcagcgtca 720

gttacagacc agagagtcgc cttcgccact ggtgttcctc cacatctcta cgcatttcac 780

cgctacacgt ggaattccac tctcctcttc tgcactcaag ttccccagtt tccaatgacc 840

ctccccggtt gagccggggg ctttcacatc agacttaaga aaccgcctgc gagcccttta 900

cgcccaataa ttccggacaa cgcttgccac ctacgtatta ccgcggctgc tggcacgtag 960

ttagccgtgg ctttctggtt aggtaccgtc aaggtaccgc cctattcgaa cggtacttgt 1020

tcttccctaa caacagagct ttacgatccg aaaaccttca tcactcacgc ggcgttgctc 1080

cgtcagactt tcgtccattg cggaagattc cctactgctg cctcccgtag gagtctgggc 1140

cgtgtctcag tcccagtgtg gccgatcacc ctctcaggtc ggctacgcat cgttgccttg 1200

gtgagccgtt acctcaccaa ctagctaatg cgccgcgggt ccatctgtaa gtggtagccg 1260

aagccacctt ttatgtttga accatgcggt tcaaacaacc atccggtatt agccccggtt 1320

tcccggagtt atcccagtct tacaggcagg ttacccacgt gttactcacc cgtccgccgc 1380

taacatcagg gagcaagctc ccatctgtcc gctcgactgc atgatagc 1428

Claims (5)

1. the used bacterial classification of this microbial fertilizer is subtilis PGPR-802, is located away from Kou Bu town, Qingzhou in Shandong province city wheat field, by the preservation at Chinese common micro-organisms preservation center, and deposit number CGMCC No.6048.

2. this kind microbial fertilizer contains following active ingredients: 10% fermentation of bacillus subtilis bacterium liquid, 40% humic acids, 50% active sludge powder.

3. this kind subtilis bacterium liquid adopts following substratum and culture parameters fermentation to form, and has sprayable characteristic: peptone 0.5%, and yeast powder 0.5%, glucose 2%, corn steep liquor 1%, NaCl 0.2%, transfers 7.5,35 ℃ of pH to be cultured to the product gemma.

4. this microbial fertilizer adopts following processes to form: at first with humic acids and the active sludge ratio mixing and stirring in 4:5, use the disc granulation technology, substratum fermentation in the employing right 2 obtains the spraying of subtilis bacterium liquid and is bonded to particle, and employing is lower than 70 ℃ of oven drying at low temperatures.

5. the application of this microbial fertilizer is the volume increase of apple, tomato.