CN102566024A - Fluorescence microscope capable of controlling light source by use of touch screen system - Google Patents

Abstract

Translated fromChinese

本发明公开了一种使用触摸屏系统控制光源的荧光显微镜，包括一采用了无限远多重色差校正光学系统的显微镜主机，所述显微镜主机上设置有一采用了高分辨率平场无限远大数值孔径的荧光物镜、一配置有单反数码相机的三目镜观察系统、一具有等高锐化特性，透过率为90％-95％的荧光滤色片组、一作为标本背景光来源的LED白光光源，以及一用于将激发光发射到标本上的LED荧光激发光源，所述显微镜主机还配置有一用于控制所述LED荧光激发光源和LED白光光源亮度的触摸屏。触摸屏作为本发明的荧光显微镜光源的调控设备，它是目前最简单、方便、自然的一种人机交互方式。

The invention discloses a fluorescent microscope using a touch screen system to control a light source, which includes a microscope host adopting an infinity multiple chromatic aberration correction optical system, and the microscope host is provided with a fluorescence microscope adopting a high-resolution flat-field infinity and a large numerical aperture. An objective lens, a trinocular observation system equipped with a digital SLR camera, a fluorescence filter set with contour sharpening characteristics and a transmittance of 90%-95%, an LED white light source as the background light source of the specimen, and An LED fluorescence excitation light source for emitting excitation light to the sample, and the microscope host is also equipped with a touch screen for controlling the brightness of the LED fluorescence excitation light source and the LED white light source. As the control device of the fluorescent microscope light source of the present invention, the touch screen is currently the simplest, most convenient and natural human-computer interaction mode.

Description

Translated fromChinese

一种使用触摸屏系统控制光源的荧光显微镜A Fluorescence Microscope Using a Touch Screen System to Control the Light Source

技术领域technical field

本发明涉及一种光学显微镜，具体涉及一种用于观察标本所发荧光的使用触摸屏系统控制光源的荧光显微镜。The invention relates to an optical microscope, in particular to a fluorescent microscope which uses a touch screen system to control a light source for observing the fluorescence emitted by a specimen.

背景技术Background technique

观测其上的标本所发荧光的荧光显微镜是显微镜的一种类型。它是荧光检测的专用工具，属于高科技仪器。荧光显微镜是以紫外线为光源，用以照射被检物体，使之发出荧光，然后在显微镜下观察物体的形状及其所在位置。荧光显微镜用于研究细胞内物质的吸收、运输、化学物质的分布及定位等。细胞中有些物质，如叶绿素等，受紫外线照射后可发荧光；另有一些物质本身虽不能发荧光，但如果用荧光染料或荧光抗体染色后，经紫外线照射亦可发荧光，荧光显微镜就是对这类物质进行定性和定量研究的工具之一。荧光显微镜的基本结构包括把激发光照射到标本上的落射光照部分和观测由所述标本所发荧光行成的图像的荧光观测部分。来自落射光照部分的激发光通过滤光器照射在标本上。所述荧光观测部分包括目镜观测系统和/或诸如CCD之类的成像部分。A fluorescence microscope, which observes fluorescence from a specimen on it, is a type of microscope. It is a special tool for fluorescence detection and is a high-tech instrument. Fluorescence microscope uses ultraviolet light as the light source to irradiate the object under inspection to make it emit fluorescence, and then observe the shape and location of the object under the microscope. Fluorescence microscopy is used to study the absorption and transportation of substances in cells, the distribution and localization of chemical substances, etc. Some substances in cells, such as chlorophyll, can fluoresce after being irradiated by ultraviolet rays; although some substances themselves cannot fluoresce, they can also fluoresce after being stained with fluorescent dyes or fluorescent antibodies after being irradiated by ultraviolet rays. One of the tools for qualitative and quantitative research of such substances. The basic structure of a fluorescence microscope includes an epi-illumination section that irradiates excitation light onto a specimen, and a fluorescence observation section that observes an image formed by fluorescence emitted by the specimen. Excitation light from the epi-illumination section shines on the specimen through a filter. The fluorescence observation part includes an eyepiece observation system and/or an imaging part such as a CCD.

荧光显微镜依照其独特的性能，应用广泛，是细菌学、病毒学、细胞学、肿瘤学、遗传学、免疫学、免疫病例学、寄生虫学等研究工作的理想仪器，可供科研、高效、医疗、防疫等部门使用，与社会发展息息相关。Fluorescence microscope is widely used according to its unique performance, and it is an ideal instrument for research work such as bacteriology, virology, cytology, oncology, genetics, immunology, immunology, parasitology, etc. It can be used for scientific research, high efficiency, The use of medical, epidemic prevention and other departments is closely related to social development.

参见图1所示，一般的使用触摸屏系统控制光源的荧光显微镜的荧光激发原理如下：Referring to Figure 1, the principle of fluorescence excitation of a general fluorescence microscope using a touch screen system to control the light source is as follows:

激发光源部分：提供激发荧光所需足够强的激发光(较短波长光线)。荧光滤色片组包括一激发滤光片和一遏止滤光片，其中所述激发滤光片：只通过相应标本需要的激发光，而不要的光线都被截止掉；所述遏止滤光片：遏止掉多余的激发光线，使通过标本中激发出来的荧光便于观察。荧光标本：被荧光色素染色过的标本。Excitation light source part: provide sufficient excitation light (shorter wavelength light) required to excite fluorescence. The fluorescent color filter set includes an excitation filter and a suppression filter, wherein the excitation filter: only passes the excitation light required by the corresponding specimen, and all unnecessary light is cut off; the suppression filter : Suppresses excess excitation light, making the fluorescence excited by the specimen easy to observe. Fluorescent specimens: Specimens that have been stained with fluorescent pigments.

其中，荧光光源是荧光显微镜的关键零部件。它需要提供特定激发光波长范围及足够光效能量，以保证检测样品得到足够的激发而发出强的荧光。Among them, the fluorescent light source is the key component of the fluorescence microscope. It needs to provide a specific excitation light wavelength range and sufficient light energy to ensure that the detection sample is sufficiently excited to emit strong fluorescence.

传统荧光光源也有使用卤素灯、氙灯等用来照明，但它们都有明显的缺点：寿命短，需经常更换灯泡；每次使用都需要等待预热一段时间；能耗大；含有铅、汞灯污染环境并对使用人员有害的物质。传统光源多采用机械式旋钮进行不同激发波长之间的切换，以及亮度的调节。但是机械式调节存在缺点：比如调节量不够细化、不精确，操作不够智能化，不方便等等。Traditional fluorescent light sources also use halogen lamps, xenon lamps, etc. for lighting, but they all have obvious disadvantages: short life, frequent replacement of bulbs; need to wait for a period of time to warm up each time they are used; high energy consumption; lamps containing lead and mercury Substances that pollute the environment and are harmful to users. Traditional light sources mostly use mechanical knobs to switch between different excitation wavelengths and adjust brightness. However, there are disadvantages in mechanical adjustment: for example, the adjustment amount is not detailed enough, it is not accurate, the operation is not intelligent enough, and it is inconvenient, etc.

发明内容Contents of the invention

为克服现有技术中的不足，本发明的目的在于提供一种使用触摸屏系统控制光源的荧光显微镜，该使用触摸屏系统控制光源的荧光显微镜可以有效避免现有技术中的缺点。In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a fluorescent microscope using a touch screen system to control the light source, which can effectively avoid the disadvantages in the prior art.

为了解决上述技术问题，实现上述目的，本发明采用了如下技术方案：In order to solve the above-mentioned technical problems and achieve the above-mentioned purpose, the present invention adopts the following technical solutions:

一种使用触摸屏系统控制光源的荧光显微镜，包括一采用了无限远多重色差校正光学系统的显微镜主机，所述显微镜主机上设置有一采用了高分辨率平场无限远大数值孔径的荧光物镜、一配置有单反数码相机的三目镜观察系统、一具有等高锐化特性，透过率为90％-95％的荧光滤色片组、一作为标本背景光来源的LED白光光源，以及一用于将激发光发射到标本上的LED荧光激发光源，所述显微镜主机还配置有一用于控制所述LED荧光激发光源和LED白光光源亮度的触摸屏。A fluorescent microscope using a touch screen system to control a light source, comprising a microscope host adopting an infinity multiple chromatic aberration correction optical system, the microscope host is provided with a fluorescence objective lens adopting a high-resolution flat-field infinity large numerical aperture, a configuration There is a trinocular observation system of a digital SLR camera, a fluorescent filter set with contour sharpening characteristics and a transmittance of 90%-95%, an LED white light source as the background light source of the specimen, and a The excitation light is emitted to the LED fluorescence excitation light source on the specimen, and the microscope host is also equipped with a touch screen for controlling the brightness of the LED fluorescence excitation light source and the LED white light source.

进一步的，所述LED荧光激发光源和LED白光光源分别通过一LED光源控制模块连接在所述触摸屏上；所述LED光源控制模块包括，一为LED荧光激发光源和LED白光光源提供可靠、恒定驱动电流的LED驱动电路模块、一用于检测用户触摸位置的触摸检测电路，一用于驱动触摸屏的触摸屏驱动电路模块，一控制触摸屏的触摸屏控制电路模块，以及一为所述LED驱动电路模块、触摸检测电路和触摸屏驱动电路模块提供稳定可靠电压电流的电源电路模块。Further, the LED fluorescence excitation light source and the LED white light source are respectively connected to the touch screen through an LED light source control module; the LED light source control module includes: providing a reliable and constant drive for the LED fluorescence excitation light source and the LED white light source A current LED drive circuit module, a touch detection circuit for detecting the user's touch position, a touch screen drive circuit module for driving the touch screen, a touch screen control circuit module for controlling the touch screen, and a touch screen for the LED drive circuit module, touch The detection circuit and the touch screen drive circuit module provide a stable and reliable voltage and current power supply circuit module.

本发明的使用触摸屏系统控制光源的荧光显微镜的LED光源控制模块的工作过程如下：The working process of the LED light source control module of the fluorescent microscope using the touch screen system to control the light source of the present invention is as follows:

当电源电路模块通电后，LED驱动电路模块、触摸屏检测电路、触摸屏驱动电路模块、触摸屏控制电路模块获得电压。When the power supply circuit module is powered on, the LED drive circuit module, the touch screen detection circuit, the touch screen drive circuit module, and the touch screen control circuit module obtain voltage.

当触摸屏检测电路检测到用户触摸位置后送触摸屏控制电路，如触摸屏显示荧光激发光源亮度数据为0，LED荧光激发光源无输出，用触摸屏上的亮度调节模块对光源亮度进行调节，当LED驱动电路模块达到一定电压启动数值后，LED荧光激发光源输出；所述LED白光光源的工作模式如LED荧光激发光源的工作模式一样。触摸屏进行有效、实时的显示。When the touch screen detection circuit detects the position touched by the user, it will be sent to the touch screen control circuit. For example, the brightness data of the fluorescent excitation light source displayed on the touch screen is 0, and the LED fluorescence excitation light source has no output. Use the brightness adjustment module on the touch screen to adjust the brightness of the light source. When the LED drive circuit After the module reaches a certain voltage starting value, the LED fluorescent excitation light source outputs; the working mode of the LED white light source is the same as that of the LED fluorescent excitation light source. Touch screen for effective and real-time display.

当需要另外一种荧光光源时，可通过触摸屏检测电路进行控制，如当系统需要绿光时，可通过触摸屏检测电路切换到绿光工作状态，此时相应的LED荧光激发光源转换为绿色，相应的LED绿指示灯亮；同理，如当系统需要蓝光时，可通过触摸屏检测电路切换到蓝光工作状态，此时相应的LED荧光激发光源转换为蓝色，此时相应的LED蓝指示灯亮。在切换过程中，由于LED的响应及LED的驱动响应时间都是ns级，因此，这种延迟可以满足荧光光源的使用要求。When another fluorescent light source is needed, it can be controlled through the touch screen detection circuit. For example, when the system needs green light, it can be switched to the green light working state through the touch screen detection circuit. At this time, the corresponding LED fluorescent excitation light source is converted to green, corresponding The LED green indicator light is on; similarly, if the system needs blue light, it can switch to the blue light working state through the touch screen detection circuit. At this time, the corresponding LED fluorescent excitation light source is converted to blue, and the corresponding LED blue indicator light is on. During the switching process, since the response time of the LED and the driving response time of the LED are both at the ns level, this delay can meet the requirements for the use of the fluorescent light source.

与现有技术相比，本发明具有以下优点：Compared with the prior art, the present invention has the following advantages:

触摸屏具有坚固耐用、反应速度快、节省空间、易于交流等很多优点。触摸屏作为本发明的荧光显微镜光源的调控设备，它是目前最简单、方便、自然的一种人机交互方式。The touch screen has many advantages such as ruggedness, fast response, space saving, and easy communication. As the control device of the fluorescent microscope light source of the present invention, the touch screen is currently the simplest, most convenient and natural human-computer interaction mode.

上述说明仅是本发明技术方案的概述，为了能够更清楚了解本发明的技术手段，并可依照说明书的内容予以实施，以下以本发明的较佳实施例并配合附图详细说明如后。本发明的具体实施方式由以下实施例及其附图详细给出。The above description is only an overview of the technical solutions of the present invention. In order to understand the technical means of the present invention more clearly and implement them according to the contents of the description, the preferred embodiments of the present invention and accompanying drawings are described in detail below. The specific embodiment of the present invention is given in detail by the following examples and accompanying drawings.

附图说明Description of drawings

图1为荧光显微镜的荧光激发原理图。Figure 1 is a schematic diagram of the fluorescence excitation principle of the fluorescence microscope.

图2为本发明的使用触摸屏系统控制光源的荧光显微镜的结构示意图。FIG. 2 is a structural schematic diagram of a fluorescence microscope using a touch screen system to control a light source of the present invention.

图3为本发明的LED光源控制模块的框架示意图。Fig. 3 is a schematic frame diagram of the LED light source control module of the present invention.

图中标号说明：1、显微镜主机，2、触摸屏，3、荧光物镜，4、三目镜观察系统，5、荧光滤色片组，6、LED荧光激发光源，7、LED白光光源，8、LED光源控制模块，801、LED驱动电路模块，802、触摸检测电路，803、触摸屏驱动电路模块，804、触摸屏控制电路模块，805、电源电路模块。Explanation of symbols in the figure: 1. Microscope host, 2. Touch screen, 3. Fluorescent objective lens, 4. Trinocular observation system, 5. Fluorescent filter set, 6. LED fluorescent excitation light source, 7. LED white light source, 8. LED Light source control module, 801, LED drive circuit module, 802, touch detection circuit, 803, touch screen drive circuit module, 804, touch screen control circuit module, 805, power circuit module.

具体实施方式Detailed ways

下面结合附图和实施例对本发明的技术实施过程做进一步说明。The technical implementation process of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

参见图2所示，一种使用触摸屏系统控制光源的荧光显微镜，包括一采用了无限远多重色差校正光学系统的显微镜主机1，所述显微镜主机1上设置有一采用了高分辨率平场无限远大数值孔径的荧光物镜3、一配置有单反数码相机的三目镜观察系统4、一具有等高锐化特性，透过率为90％-95％的荧光滤色片组5、一作为标本背景光来源的LED白光光源7，以及一用于将激发光发射到标本上的LED荧光激发光源6，所述显微镜主机1还配置有一用于控制所述LED荧光激发光源6和LED白光光源7亮度的触摸屏2。Referring to Fig. 2, a fluorescent microscope using a touch screen system to control the light source includes amicroscope host 1 that adopts an infinity multiple chromatic aberration correction optical system. Themicroscope host 1 is provided with a high-resolution flat-field infinity large Fluorescence objective lens withnumerical aperture 3, a trinocular observation system equipped with a digital SLR camera 4, a fluorescent filter set with contour sharpening characteristics and a transmittance of 90%-95% 5, a background light for the specimen source of LEDwhite light source 7, and an LED fluorescence excitation light source 6 for emitting excitation light onto the specimen, and themicroscope host 1 is also equipped with a device for controlling the brightness of the LED fluorescence excitation light source 6 and LEDwhite light source 7 touch screen2.

进一步的，参见图3所示，所述LED荧光激发光源6和LED白光光源7分别通过一LED光源控制模块8连接在所述触摸屏2上；所述LED光源控制模块8包括，一为LED荧光激发光源6和LED白光光源7提供可靠、恒定驱动电流的LED驱动电路模块801、一用于检测用户触摸位置的触摸检测电路802，一用于驱动触摸屏2的触摸屏驱动电路模块803，一控制触摸屏2的触摸屏控制电路模块804，以及一为所述LED驱动电路模块801、触摸检测电路802和触摸屏驱动电路模块803提供稳定可靠电压电流的电源电路模块805。Further, referring to FIG. 3 , the LED fluorescent excitation light source 6 and the LEDwhite light source 7 are respectively connected to thetouch screen 2 through an LED lightsource control module 8; the LED lightsource control module 8 includes, one is an LED fluorescent The excitation light source 6 and the LEDwhite light source 7 provide a reliable, constant drive current LEDdrive circuit module 801, atouch detection circuit 802 for detecting the user's touch position, a touch screendrive circuit module 803 for driving thetouch screen 2, and a touch screendrive circuit module 803 for controlling thetouch screen 2 touch screencontrol circuit module 804, and a powersupply circuit module 805 that provides stable and reliable voltage and current for the LEDdrive circuit module 801,touch detection circuit 802 and touch screendrive circuit module 803.

上述实施例只是为了说明本发明的技术构思及特点，其目的是在于让本领域内的普通技术人员能够了解本发明的内容并据以实施，并不能以此限制本发明的保护范围。凡是根据本发明内容的实质所作出的等效的变化或修饰，都应涵盖在本发明的保护范围内。The above-mentioned embodiments are only to illustrate the technical concept and characteristics of the present invention, and its purpose is to enable those of ordinary skill in the art to understand the content of the present invention and implement it accordingly, and cannot limit the protection scope of the present invention. All equivalent changes or modifications made according to the essence of the present invention shall fall within the protection scope of the present invention.

Claims (2)

Translated fromChinese

1.一种使用触摸屏系统控制光源的荧光显微镜，包括一采用了无限远多重色差校正光学系统的显微镜主机(1)，所述显微镜主机(1)上设置有一采用了高分辨率平场无限远大数值孔径的荧光物镜(3)、一配置有单反数码相机的三目镜观察系统(4)、一具有等高锐化特性，透过率为90％-95％的荧光滤色片组(5)、一作为标本背景光来源的LED白光光源(7)，以及一用于将激发光发射到标本上的LED荧光激发光源(6)，其特征在于：所述显微镜主机(1)还配置有一用于控制所述LED荧光激发光源(6)和LED白光光源(7)亮度的触摸屏(2)。1. A fluorescent microscope using a touch screen system to control the light source, comprising a microscope host (1) that adopts an infinity multiple chromatic aberration correction optical system, and a microscope host (1) that adopts a high-resolution flat-field infinity lens is arranged on the microscope host (1). A fluorescent objective lens with numerical aperture (3), a trinocular observation system (4) equipped with a digital SLR camera, a fluorescent color filter set (5) with contour sharpening characteristics and a transmittance of 90%-95% . An LED white light source (7) as a source of background light for the specimen, and an LED fluorescence excitation light source (6) for emitting excitation light to the specimen, characterized in that: the microscope host (1) is also equipped with a The touch screen (2) is used for controlling the brightness of the LED fluorescent excitation light source (6) and the LED white light source (7).2.根据权利要求1所述的使用触摸屏系统控制光源的荧光显微镜，其特征在于：所述LED荧光激发光源(6)和LED白光光源(7)分别通过一LED光源控制模块(8)连接在所述触摸屏(2)上；所述LED光源控制模块(8)包括，一为LED荧光激发光源(6)和LED白光光源(7)提供可靠、恒定驱动电流的LED驱动电路模块(801)、一用于检测用户触摸位置的触摸检测电路(802)，一用于驱动触摸屏(2)的触摸屏驱动电路模块(803)，一控制触摸屏(2)的触摸屏控制电路模块(804)，以及一为所述LED驱动电路模块(801)、触摸检测电路(802)和触摸屏驱动电路模块(803)提供稳定可靠电压电流的电源电路模块(805)。2. The fluorescence microscope using a touch screen system to control the light source according to claim 1, characterized in that: the LED fluorescence excitation light source (6) and the LED white light source (7) are respectively connected to the LED light source control module (8) On the touch screen (2); the LED light source control module (8) includes an LED drive circuit module (801) that provides a reliable and constant drive current for the LED fluorescent excitation light source (6) and the LED white light source (7), A touch detection circuit (802) for detecting a user's touch position, a touch screen drive circuit module (803) for driving the touch screen (2), a touch screen control circuit module (804) for controlling the touch screen (2), and a The LED drive circuit module (801), touch detection circuit (802) and touch screen drive circuit module (803) provide a stable and reliable voltage and current power supply circuit module (805).

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