The method of a kind of composite carrier immobilized ammonia oxidizing bacteriaTechnical field:
The present invention relates to a kind of ammonia nitrogen oxidized form microbial immobilization technology, belong to microbial fermentation and water treatment field.
Background technology:
In the conventional WWT, denitrogenation is important link, at AO, and A2In the water technologies such as O, ammonia nitrogen normal open peroxide turns to nitric acid, is reduced to nitrogen after nitrification liquid refluxes and removes.In this reaction process, the most key conditioning step is the oxidation of ammonia nitrogen.The oxidation of ammonia nitrogen needs three conditions, and one is sufficient dissolved oxygen, and the 2nd, lower COD concentration, the 3rd, a large amount of ammonia oxidizing bacteria (nitrobacteria).Nitrobacteria has two types, and one type is ammonia oxidizing bacteria, and another kind of is nitrite-oxidizing bacteria.As far as denitrifying microorganism, the product nitrite of ammonia oxidizing bacteria just can be used as the denitrogenation substrate, so ammonia nitrogen is oxidizing to this stage denitrification that can reflux.
For a reactor drum reasonable in design, create suitable dissolved oxygen and realize that under higher COD concentration the important link of nitration reaction possesses enough concentration and nitrobacteria efficiently exactly.And microbial immobilized technology is the direct method of possessing enough concentration nitrobacterias.Microbial immobilized technology is exactly to adopt suitable carriers that mikrobe purebred or that separate to confirm is fixed on certain space, makes it not diluted with current, takes away, and can guarantee in the reactor drum biocatalytic reaction efficiently like this.
The fixation support that is suitable for wastewater treatment should have following performance: 1) nontoxic to mikrobe; 2) mass-transfer performance is good; 3) stable in properties; 4) cost simple for production is low.
Summary of the invention:
The present invention has developed and has a kind ofly utilized complex carrier to be fixed as the product of microcapsule ammonia oxidizing bacteria.The bacterium of this product embedding is the ammonia oxidizing bacteria of purebred cultivation.Strain separating is from the nitrated segment process part of sewage work.Fixation support is that PVA and gac are main mixture.
Microbial cultivation process:, be in charge of shake-flask seed, seeding tank, fermentor tank through slant tube.This process is in this special introduction, and the professional of can generally being fermented understands this process.
The mikrobe fixing means: 1. the ammonia oxidizing bacteria that fermentation is spread cultivation out is centrifugal, centrifugal speed 4000～10000g.Bacterium mud is collected the back and is adopted the above activity charcoal powder mixing and absorption of 1000 orders, activity charcoal powder and bacterium mud volume ratio 100: 1～1: 1, and the mixture after stirring is for use 4 ℃ of preservations.2. PVA is prepared as 10% the aqueous solution, and with bacterium mud mixture, CMC and this aqueous solution thorough mixing.This mixture is splashed in the saturated BAS, place more than the 10h, it is fully reacted.3. leach immobilization particle, clean, be placed on 4 ℃ of preservations with saline water.
Need adopt the grid thing to isolate immobilization particle during use, in order to avoid taken out of by water, and place reactor drum air water stream to impact less space as far as possible this immobilization particle as far as possible.