A1, A2, A3, A4 are respectively in the table 1,2,3, No. 4 reactant liquor and to be measured group of solution in 20 ℃ in the formula, after 3.5 hours, and the absorbance under the 475nm wavelength.

The 1st group of solution preparation:

Reactant liquor	Solution	1	Solution 2	Solution 3	Solution 4
						0.1%L-tyrosine	0.5	0	0.5	0
The 1st group oftesting sample	0	0	1.0	1.0
					Tryrosinase solution	0.5	0.5	0.5	0.5
The phosphate buffer solution of PH6.8	1.5	2.0	1.5	2.0
					Add up to	3.5	3.5	3.5	3.5

The 2nd group of solution preparation:

Reactant liquor	Solution	1	Solution 2	Solution 3	Solution 4
						0.1%L-tyrosine	0.5	0	0.5	0
The 2nd group oftesting sample	0	0	1.0	1.0
					Tryrosinase solution	0.5	0.5	0.5	0.5
The phosphate buffer solution of PH6.8	1.5	2.0	1.5	2.0
					Add up to	3.5	3.5	3.5	3.5

The 3rd group of solution preparation:

Reactant liquor	Solution	1	Solution 2	Solution 3	Solution 4
						0.1%L-tyrosine	0.5	0	0.5	0
The 3rd group oftesting sample	0	0	1.0	1.0
					Tryrosinase solution	0.5	0.5	0.5	0.5
The phosphate buffer solution of PH6.8	1.5	2.0	1.5	2.0
					Add up to	3.5	3.5	3.5	3.5

The 4th group of solution preparation:

Reactant liquor	Solution	1	Solution 2	Solution 3	Solution 4
						0.1%L-tyrosine	0.5	0	0.5	0
The 4th group oftesting sample	0	0	1.0	1.0
					Tryrosinase solution	0.5	0.5	0.5	0.5
The phosphate buffer solution of PH6.8	1.5	2.0	1.5	2.0
					Add up to	3.5	3.5	3.5	3.5

The 5th group of solution preparation:

Reactant liquor	Solution	1	Solution 2	Solution 3	Solution 4
						0.1%L-tyrosine	0.5	0	0.5	0
The 5th group oftesting sample	0	0	1.0	1.0
					Tryrosinase solution	0.5	0.5	0.5	0.5
The phosphate buffer solution of PH6.8	1.5	2.0	1.5	2.0
					Add up to	3.5	3.5	3.5	3.5

5, experiment detects

Use ultraviolet spectrophotometer to measure the ultraviolet absorptivity at 475nm place take TYR as substrate.

6, result of the test

Project	The 1st group	The 2nd group	The 3rd group	The 4th group	The 5th group
						Average suppression ratio %	45.6	78.5	90.6	96.8	83.1

Above experimental result has shown that Whitening, spot cosmetic composition of the present invention is can restraint of tyrosinase active, prevents that melanin from generating.And result of the test also shows, wherein the inhibition of the 4th group of testing sample is than other prescription unexpectedly optimal effectiveness intentionally, up to 96.8%.In addition, 1 pair of tryrosinase of embodiment has certain inhibitory action in the 5th group of testing sample Chinese patent " 200410051908.1 " the composite whitening spot-dispelling compositions.

Three, melanin reduction test

1, experimental principle

Experiment in vitro carries out reduction to established melanocyte by experiment, then observes the melanoma cell change color.Thereby determine the vat black pigment effect of compositions.

2, experimental technique

The B16 melanoma cell,embodiment 1 solution in

embodiment

1,2,3,4, Chinese patent " 200410051908.1 " the composite whitening spot-dispelling compositions, MEM culture fluid.

B16 melanotic tumor cell is cultivated 24h in culture fluid, then add 1,3-2-N-dimethylpurine and continue constant temperature culture 24h.

Whitening, spot cosmetic composition 5ml joined respectively contain in the melanomatous MEM test-tube culture medium of B16 (containing 10%FBS) and cultivate, 37 ℃ of constant temperature are cultivated 28h, use at last the TCA dissolved cell, and measure melanin content in the cell.The melanin synthetic ratio of untreated cell is calculated as 100%.

3, result of the test

Above experimental result has shown that Whitening, spot cosmetic composition of the present invention has reproducibility to established melanin, can be with established melanin reduction desalination.And result of the test shows that also the 4th group of testing sample also has unexpected optimal effectiveness to melanic reduction than other prescription.Equally,embodiment 1 sample is very weak to melanic reducing power in the 5th group of testing sample Chinese patent " 200410051908.1 " the composite whitening spot-dispelling compositions.

Four, suppress established melanin transfer to the epidermis cell test

1, experimental principle

Melanocyte can produce melanin, and melanin granule shifts to epithelial cells by the dendritic projection of melanocyte, and the melanin granule that is transferred to epithelial cells goes upward to horny layer with epidermis cell, thereby forms mottle at skin.Therefore, to the epidermis cell transmission, solve melanin transfer by the check melanin granule.

2, experimental technique

With epithelial cells and melanocyte co-cultivation, to be measured group of solution was constant temperature culture 6 days more than adding respectively in culture.The blank group only is epithelial cells and melanocyte co-cultivation.

4, result of the test

	Matched group	The 1st group	The 2nd group	The 3rd group	The 4th group	The 5th group
							Melanin content %	86.4	76.8	45.8	53.3	32.7	74.5
Suppression ratio %	0	11.1	47.0	38.3	62.1	5.8

Above data show that this Whitening, spot cosmetic composition has the effect that obvious check melanin granule shifts to epidermis.And wherein the inhibition of the 4th group of testing sample is than other prescription unexpectedly optimal effectiveness intentionally, and its suppression ratio is up to 62.1%.And the 5th group of testing sample only has 5.8% to the suppression ratio of melanin transfer.

Whitening effect test between commercially available whitening essence sample A and the embodiment of the invention 7 sample B

1, experimental principle

Human experimentation is chosen common volunteer and is formed tested crowd, divides 4 groups; Choose volunteer's number 15 people for every group, wherein the male is 4,11 of women, and the age, test subject made the variation of the front and back skin color that applies some make up between 20～40 years old, thereby determined the white-skinned face function of Whitening, spot cosmetic composition of the present invention.

2, experimental apparatus and sample

Instrument: dermal melanin and haemachrome tester (Mexameter MX18, German CK electronics corporation produces); Lab color difference meter (MPA9, German CK electronics corporation produces).

Sample: commercially available whitening essence sample A (containing the arbutin whitening composition), the embodiment of the invention 7 outturn sample B.

Testing location: plant resources research and development key lab of Beijing Technology and Business University

3, experiment crowd and testing time

Volunteer referring to experimentdivides 3 groups, every group of totally 15 people, and wherein the male is 4,11 of women, the age was at 20～40 years old.The 1st group is the blank group; Test commercially available whitening essence sample A for the 2nd group; The 3rd group of test embodiment of the invention 7 outturn sample B;

Volunteer service time: respectively smear once every day sooner or later;

Testing time is one month.

4, experimental technique

Before the experimenter smears sample, will try first the experiment position and clean, smear sample after drying.The left and right arm of experimenter inboard is apart from palm base portion 5cm place, and each determines the area of 4 * 4cm size as the experiment position, and sample is applied in the left arm inboard, and sample B is applied in the right arm inboard.

The experimenter smears dosage in each test block be 50 μ L/ time (about two of disposable test tube), and respectively smear once every day sooner or later.Experimental session, the experimenter can not smear other any cosmetics at the experiment position.

5, experiment detects

The experimenter make continuously apply some make up after same time weekly, use dermal melanin and haemachrome tester and Lab color difference meter to test the variation of skin color, average.

6, result of the test

Experimenter's melanin content is changed drawing, see accompanying drawing 1.1 as can be known with reference to the accompanying drawings, and commercially available sample A and sample B of the present invention all have preferably check melanin ability, and the effect that the check melanin of sample B generates slightly is better than sample A, and blank group has clear improvement.

Experimenter's skin brightness (L) value is changed drawing, see accompanying drawing 2,2 as can be known with reference to the accompanying drawings, and commercially available sample A and sample B of the present invention all have the ability of better increase skin brightness, and the effect of sample B is better than sample A, and blank group has clear improvement.

Obviously, the above embodiment of the present invention only is for example of the present invention clearly is described, and is not to be restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here can't give all embodiments exhaustive.Everyly belong to the row that apparent variation that technical scheme of the present invention extends out or change still are in protection scope of the present invention.

Claims

1. a Whitening, spot cosmetic composition is characterized in that, is comprised of the raw material of following parts by weight:

0.5～20 part of nicotiamide,

0.1～15 part of AA 2G,

0.1～10 part of yeast extract,

0.05～10 part of sodium lactate/gluconic acid sodium salt,

0.01～3.5 part of sodium hydroxide,

10～60 parts in water.

2. described Whitening, spot cosmetic composition according to claim 1 is characterized in that, is comprised of the raw material of following parts by weight: 20 parts of nicotiamide, 10 parts of AA 2Gs, 5 parts of yeast extracts, 5 parts of sodium lactate/gluconic acid sodium salts, 2.7 parts of sodium hydroxide; 57 parts in water.

3. described Whitening, spot cosmetic composition according to claim 1 is characterized in that amino acid content is more than 30% in the described yeast extract, and total protein content is more than 50%, and nucleotide content is more than 10%.

4. an essence that contains just like arbitrary described Whitening, spot cosmetic composition among the claim 1-3 is characterized in that, comprises the raw material of following percentage by weight:

GI CD-10 10～30%，

Butanediol 5～30%,

Glycerol 5%,

Gransurf 50C 0.5～1.5%，

PE 9010 0.6%,

Gransurf 67 0.5～1.5%,

CM040 1～5%,

GTCC 1～4%，

Dl-alpha-tocopherol acetate 0.1～0.5%,

Gransil VX-401 2.5%,

Whitening, spot cosmetic composition 20%,

Radix Ginseng extract 0.1%,

Fructus Pruni pseudocerasi extract 0.1%,

Glycyrrhizic acid dipotassium 0.03%,

Herba Saxifragae extract 0.1%,

Xanthan gum 0.1%,

Add water to total amount 100%;

International cosmetic raw material called after ring five polydimethylsiloxane of described GI CD-10, the poly-first siloxanes of stearic oxygen/dimethione copolymer and polydimethylsiloxane;

International cosmetic raw material called after polydimethylsiloxane and the PEG/PPG-18/18 polydimethylsiloxane of described Gransurf 50C;

International cosmetic raw material called after phenoxyethanol/Sensiva SC50 of described PE 9010;

The international cosmetic raw material called after PEG-10 polydimethylsiloxane of described Gransurf 67;

International cosmetic raw material called after ring five polydimethylsiloxane of described CM040;

The international cosmetic raw material called after caprylic/capric triglyceride of described GTCC;

The poly-first siloxanes of the stearic oxygen of the international cosmetic raw material called after of described Gransil VX-401/dimethione copolymer.

5. essence according to claim 4 is characterized in that, described Radix Ginseng extract is the Radix Ginseng root extract.

6. essence according to claim 4 is characterized in that, described Fructus Pruni pseudocerasi extract is the gean berry extract.

7. essence according to claim 4 is characterized in that, described Herba Saxifragae extract is the aaron's beard extract.

8. a gentle skin liquid that contains just like arbitrary described Whitening, spot cosmetic composition among the claim 1-3 is characterized in that, comprises the raw material of following percentage by weight:

DC2501 1～5%，

Disodium EDTA 0.05%,

Xanthan gum 0.1～0.4%,

Glycerol 4%,

Antiseptic K350 0.5%,

Butanediol 6%,

Whitening, spot cosmetic composition 5%,

MG-60 0.1～1.5%，

Herba Saxifragae extract 0.01～0.5%,

Fructus Pruni pseudocerasi extract 0.1～0.5%,

Essence 0.01%,

HPS 0.04%，

Add water to 100%;

The international cosmetic raw material called after of described DC2501 is two-PEG-18 methyl ether dimethylsilane;

International cosmetic raw material called after phenoxyethanol/methyl hydroxybenzoate of described antiseptic K350/ethyl hydroxybenzoate/Sensiva SC50;

International cosmetic raw material called after Fructus Hordei Germinatus oligose glycosides/hydrogenated starch hydrolysate of described MG-60;

The international cosmetic raw material called after cocos nucifera oil alcohol ether of described HPS-7/PPG-1-PEG-9 lauryl glycol ether/Cremophor RH40.

9. gentle skin liquid according to claim 8 is characterized in that, described Fructus Pruni pseudocerasi extract is the gean berry extract.

10. gentle skin liquid according to claim 8 is characterized in that, described Herba Saxifragae extract is the aaron's beard extract.

11. the preparation method of essence as claimed in claim 4 is characterized in that, comprises the steps:

1) takes by weighing raw material by described percentage by weight;

3) Gransil VX-401, GTCC, PE 9010, CM040, dl-alpha-tocopherol acetate are heated to 50 ℃ of fully fusings, join among the above-mentioned milky paste A, be stirred to mix homogeneously; Get mixture B;

12. the preparation method of gentle skin liquid as claimed in claim 8 is characterized in that, comprises the steps:

1) takes by weighing raw material by described percentage by weight;

4) mixture H is joined among the mixture G, stir, namely get the gentle skin liquid that contains the Whitening, spot cosmetic composition.