CN102141535A - Enzyme-injection glucose sensing on-line analyzer - Google Patents

Abstract

Translated fromChinese

本发明提供了一种面向发酵过程的酶注射式葡萄糖传感在线分析仪，其包括如下部分：酶注射式葡萄糖传感在线分析仪流路结构系统1、三电极检测电路2、数据采集控制电路3、液晶屏4、PC机5、按键6、电源7。本发明采用酶液注射方式，可实现传感器随发酵罐同时进行在线高压蒸汽灭菌，不仅增强了传感器的可靠性，而且提高了检测精确度；酶以“新鲜”的酶液方式存在，与被测液独立，不受外界影响，使得仪器不需要频繁校准；更换不同种类的酶液，可以检测不同物质，即一台仪器可检测多种物质；检测成本低，透析取样探头的设计大大减少了检测中对待测液的消耗；实现了葡萄糖的在线检测；本发明，结构紧凑、加工方便，易于批量化生产。

The present invention provides an enzyme injection glucose sensor on-line analyzer for the fermentation process, which includes the following parts: enzyme injection glucose sensor on-line analyzer flow path structure system 1, three-electrode detection circuit 2, data acquisition control circuit 3. LCD screen 4. PC machine 5. Button 6. Power supply 7. The invention adopts the enzyme liquid injection method, which can realize the simultaneous online high-pressure steam sterilization of the sensor along with the fermenter, which not only enhances the reliability of the sensor, but also improves the detection accuracy; The measuring liquid is independent and not affected by the outside world, so that the instrument does not need to be frequently calibrated; changing different types of enzyme liquids can detect different substances, that is, one instrument can detect multiple substances; the detection cost is low, and the design of the dialysis sampling probe is greatly reduced. The consumption of the liquid to be tested during detection; the on-line detection of glucose is realized; the invention has compact structure, convenient processing and easy mass production.

Description

Translated fromChinese

酶注射式葡萄糖传感在线分析仪Enzyme Injection Glucose Sensing Online Analyzer

技术领域technical field

本发明涉及葡萄糖在线检测技术，是一种综合采用物理、化学、生物、电子的葡萄糖在线分析仪，具体地说，是一种面向发酵过程的酶注射式葡萄糖传感在线分析仪。The invention relates to a glucose on-line detection technology, which is an on-line glucose analyzer which comprehensively adopts physics, chemistry, biology and electronics, in particular, an enzyme-injection-type glucose sensor on-line analyzer for fermentation process.

背景技术Background technique

葡萄糖检测技术在发酵工业、食品工业、医用血糖分析上有着广泛的应用，与常见葡萄糖分析方法——斐林定糖法、葡萄糖氧化酶-过氧化物酶-比色法相比，葡萄糖生物传感器检测葡萄糖法，具有专一性强，适于在线，速度快，可连续操作，操作简便等诸多优点，故葡萄糖生物传感器测糖得到了广泛的研究与应用。Glucose detection technology is widely used in the fermentation industry, food industry, and medical blood sugar analysis. Compared with the common glucose analysis methods - Felindin method, glucose oxidase-peroxidase-colorimetric method, glucose biosensor detection Glucose method has many advantages, such as strong specificity, suitable for online, fast speed, continuous operation, easy operation, etc. Therefore, glucose biosensor for measuring sugar has been widely researched and applied.

葡萄糖是微生物发酵过程中常见的反应物和中间产物，发酵过程中对葡萄糖浓度的控制会直接关系到产物产率与质量。因此，葡萄糖浓度的实时在线检测是非常重要的。然而发酵过程中，为了防止染菌，在线葡萄糖生物传感器及其与反应器间管路必须能承受高温蒸汽灭菌，以避免灭菌后传感器性能下降或丧失，所以目前葡萄糖浓度检测主要采用离线方式，在线方式还处于实验阶段。Glucose is a common reactant and intermediate product in the microbial fermentation process. The control of glucose concentration in the fermentation process will directly affect the product yield and quality. Therefore, real-time online detection of glucose concentration is very important. However, in the fermentation process, in order to prevent bacterial contamination, the online glucose biosensor and the pipeline between it and the reactor must be able to withstand high-temperature steam sterilization to avoid sensor performance degradation or loss after sterilization, so the current glucose concentration detection mainly adopts the offline method , the online method is still in the experimental stage.

目前，葡萄糖传感器大多采用酶膜的方式，然而，酶膜制作需要酶固定化技术，即将葡萄糖氧化酶或葡萄糖脱氢酶固定于半透膜上或电极上，固定化技术复杂；酶膜的活度受被测液影响，酶膜随反应活度逐渐降低，仪器需要不断校准；并且酶膜使用前需要泡涨，用时较长，难以在线使用；一种酶膜的传感器只能检测一种物质；另外，酶膜价格昂贵。At present, most glucose sensors use enzyme membranes. However, the production of enzyme membranes requires enzyme immobilization technology, that is, glucose oxidase or glucose dehydrogenase is immobilized on semi-permeable membranes or electrodes. The immobilization technology is complicated; the activity of enzyme membranes Affected by the liquid to be tested, the enzyme membrane gradually decreases with the reaction activity, and the instrument needs to be calibrated continuously; and the enzyme membrane needs to be soaked before use, which takes a long time and is difficult to use online; a sensor of an enzyme membrane can only detect one substance ; In addition, the enzyme membrane is expensive.

本发明是为了解决上诉问题，目的在于提供一种适用于在线的，测量准确的，操作方便的，不需经常校准的，成本低的葡萄糖传感在线分析仪。The purpose of the present invention is to solve the above-mentioned problem, and the purpose is to provide an on-line glucose sensor analyzer that is suitable for on-line, accurate in measurement, easy to operate, does not require frequent calibration, and low in cost.

发明内容Contents of the invention

本发明的目的是为了提供了一种面向发酵过程的酶注射式葡萄糖传感在线分析仪。The purpose of the present invention is to provide an enzyme injection glucose sensor on-line analyzer for fermentation process.

酶注射式葡萄糖传感在线分析仪，其包括：酶注射式葡萄糖传感在线分析仪流路结构系统1、三电极检测电路2、数据采集控制电路3、液晶屏4、PC机5、按键6、电源7，具体连接关系为：Enzyme injection glucose sensor online analyzer, which includes: enzyme injection glucose sensor on-line analyzer flow path structure system 1, three-electrode detection circuit 2, dataacquisition control circuit 3,LCD screen 4, PC 5,buttons 6 ,power supply 7, the specific connection relationship is:

所述酶注射式葡萄糖传感在线分析仪流路结构系统1与三电极检测电路2和数据采集控制电路3连接，三电极检测电路2用于检测酶注射式葡萄糖传感在线分析仪流路结构系统1中酶注射式葡萄糖生物传感器8产生的电流信号，数据采集控制电路3用于给酶注射式葡萄糖传感在线分析仪流路结构系统1提供控制信号；三电极检测电路2与数据采集控制电路3连接，数据采集控制电路3用于采集三电极检测电路2输出的电压信号；数据采集控制电路3还分别与液晶屏4、PC机5、按键6相连，液晶屏4用于显示数据采集控制电路3输出的数字信号，PC机5可通过串口与数据采集控制电路3进行通信，按键6用于设置数据采集控制电路3运行参数；电源7与三电极检测电路2、数据采集控制电路3和酶注射式葡萄糖传感在线分析仪流路结构系统1相连，为它们供电。要不要把三级检测电路画一下？The flow path structure system 1 of the enzyme injection type glucose sensor on-line analyzer is connected to the three-electrode detection circuit 2 and the dataacquisition control circuit 3, and the three-electrode detection circuit 2 is used to detect the flow path structure of the enzyme injection type glucose sensor on-line analyzer The current signal generated by the enzyme-injectedglucose biosensor 8 in the system 1, the dataacquisition control circuit 3 is used to provide control signals to the flow path structure system 1 of the enzyme-injected glucose sensor on-line analyzer; the three-electrode detection circuit 2 and the data acquisition control Thecircuit 3 is connected, and the datacollection control circuit 3 is used to collect the voltage signal output by the three-electrode detection circuit 2; The digital signal output by thecontrol circuit 3, thePC 5 can communicate with the dataacquisition control circuit 3 through the serial port, and thekey 6 is used to set the operating parameters of the dataacquisition control circuit 3; thepower supply 7 and the three-electrode detection circuit 2, the dataacquisition control circuit 3 It is connected with the flow path structure system 1 of the enzyme injection glucose sensor on-line analyzer to provide power for them. Do you want to draw the three-level detection circuit?

所述酶注射式葡萄糖传感在线分析仪流路结构连接系统1包括：酶注射式葡萄糖生物传感器8、透析取样探头9、电磁六通阀10、定量环11、蠕动泵12、13、17、缓冲液瓶14、酶液瓶15、废液瓶16；具体连接关系为：所述透析取样探头9包括固定环18、外支撑网19、透析膜20、内支撑网21、探头主体22、探头入口23、探头出口24、扩散池25；The flow structure connection system 1 of the enzyme injection glucose sensor online analyzer includes: an enzymeinjection glucose biosensor 8, a dialysis sampling probe 9, an electromagnetic six-way valve 10, aquantitative loop 11, aperistaltic pump 12, 13, 17,Buffer solution bottle 14,enzyme solution bottle 15,waste solution bottle 16; the specific connection relationship is: the dialysis sampling probe 9 includes afixed ring 18, anouter support net 19, adialysis membrane 20, aninner support net 21, aprobe body 22, aprobe Inlet 23,probe outlet 24,diffusion cell 25;

所述酶注射式葡萄糖生物传感器8的三电极通过电缆连接到三电极检测电路板，实现传感器产生电流的I/V变换及放大；所述酶注射式葡萄糖生物传感器8的被测液入口通过管路与透析取样探头9的探头出口24连接，在蠕动泵17的工作下，透析取样探头9的扩散池中的待测液被引入到被测液池中；所述透析取样探头9的探头入口23通过管路与缓冲液瓶14连接，此管路经过蠕动泵17；The three electrodes of the enzyme-injectedglucose biosensor 8 are connected to the three-electrode detection circuit board through cables to realize the I/V conversion and amplification of the current generated by the sensor; the measured liquid inlet of the enzyme-injectedglucose biosensor 8 passes through the tube The road is connected with theprobe outlet 24 of the dialysis sampling probe 9, and under the work of theperistaltic pump 17, the liquid to be tested in the diffusion cell of the dialysis sampling probe 9 is introduced into the liquid pool to be tested; the probe inlet of the dialysis sampling probe 9 23 is connected withbuffer solution bottle 14 through pipeline, and this pipeline passes throughperistaltic pump 17;

所述电磁六通阀10的第6端口通过管路与缓冲液瓶14连接，此管路经过蠕动泵12；所述电磁六通阀10的第3端口通过管路与酶液瓶15连接，此管路经过蠕动泵13；所述电磁六通阀10的第2端口通过管路与废液瓶16连接；所述电磁六通阀10的第1端口与第4端口相连，之间连接定量环11；所述电磁六通阀10的第5端口通过管路与酶注射式葡萄糖生物传感器8的缓冲液、酶液入口连接；所述酶注射式葡萄糖生物传感器8的缓冲液、酶液出口通过管路与废液瓶16连接；The 6th port of described electromagnetic six-way valve 10 is connected withbuffer bottle 14 by pipeline, and this pipeline passes throughperistaltic pump 12; The 3rd port of described electromagnetic six-way valve 10 is connected with enzymeliquid bottle 15 by pipeline, This pipeline passes through theperistaltic pump 13; the second port of the electromagnetic six-way valve 10 is connected with thewaste liquid bottle 16 through the pipeline; the first port of the electromagnetic six-way valve 10 is connected with the fourth port, and thequantitative Ring 11; the fifth port of the electromagnetic six-way valve 10 is connected to the buffer solution and enzyme solution inlet of the enzymeinjection glucose biosensor 8 through pipelines; the buffer solution and enzyme solution outlet of the enzymeinjection glucose biosensor 8 Connect withwaste liquid bottle 16 by pipeline;

所述透析取样探头9的探头主体22上部开有探头入口23和探头出口24，探头主体22的底部置有内支撑网21，底部外侧固定有外支撑网19，通过固定环18将内支撑网21和外支撑网19固定在探头主体22底部；所述探头主体22与内支撑网21间构成扩散池25；所述内支撑网21与外支撑网19间固定透析膜20，内支撑网21与外支撑网19对透析膜20起加固作用；所述固定环18外圈带有螺纹，与一端带有螺纹的探头主体22螺纹连接，对外支撑网19、透析膜20、内支撑网21进行固定；所述探头入口23和探头出口24，用于液体的流入与流出。The probemain body 22 top of described dialysis sampling probe 9 hasprobe inlet 23 andprobe outlet 24, and the bottom of probemain body 22 is provided withinner support net 21, andouter support net 19 is fixed on the outside of the bottom, andinner support net 19 is fixed byfixing ring 18. 21 and theouter support net 19 are fixed on the bottom of the probemain body 22; thediffusion pool 25 is formed between the probemain body 22 and theinner support net 21; thedialysis membrane 20 is fixed between theinner support net 21 and theouter support net 19, and theinner support net 21 Thedialysis membrane 20 is reinforced with theouter support net 19; the outer ring of thefixing ring 18 has threads, and is threadedly connected with theprobe body 22 with threads at one end, and the outer support net 19, thedialysis membrane 20, and the inner support net 21 fixed; theprobe inlet 23 and theprobe outlet 24 are used for inflow and outflow of liquid.

各部件间通过管路连接，此管路的材料可选不锈钢、聚四氟乙烯、PEEK或硅胶。The components are connected by pipelines, and the material of the pipelines can be stainless steel, polytetrafluoroethylene, PEEK or silica gel.

电磁六通阀10与定量环11构成酶液定量注射装置。The electromagnetic six-way valve 10 and thequantitative loop 11 constitute an enzyme liquid quantitative injection device.

所述蠕动泵12、13、17是流量可调、正反转可调的蠕动泵。Theperistaltic pumps 12, 13, 17 are peristaltic pumps with adjustable flow rate and adjustable forward and reverse rotation.

所述探头主体22材料可选不锈钢、PEEK。The material of theprobe body 22 may be stainless steel or PEEK.

所述透析膜20，可选聚碳酸酯膜、聚砜膜。Thedialysis membrane 20 can be selected from polycarbonate membrane and polysulfone membrane.

外支撑网19、内支撑网21由不锈钢网制成。Outer support net 19,interior support net 21 are made of stainless steel net.

其中，透析膜20，允许葡萄糖分子或比其小的分子通过，阻止大分子通过。Among them, thedialysis membrane 20 allows glucose molecules or smaller molecules to pass through, and prevents macromolecules from passing through.

本发明的优点是：The advantages of the present invention are:

采用酶液注射方式，可实现传感器随发酵罐同时进行在线高压蒸汽灭菌，不仅增强了传感器的可靠性，而且提高了检测精确度；The enzyme liquid injection method can realize the online high-pressure steam sterilization of the sensor along with the fermentation tank, which not only enhances the reliability of the sensor, but also improves the detection accuracy;

酶以“新鲜”的酶液方式存在，与被测液独立，不受外界影响，使得仪器不需要频繁校准；The enzyme exists in the form of "fresh" enzyme solution, which is independent from the measured solution and free from external influences, so that the instrument does not need frequent calibration;

更换不同种类的酶液，可以检测不同物质，即一台仪器可检测多种物质；Different types of enzyme solutions can be replaced to detect different substances, that is, one instrument can detect multiple substances;

检测成本低，单次所用酶液成本0.01元左右；The detection cost is low, and the cost of a single enzyme solution used is about 0.01 yuan;

透析取样探头的设计大大减少了检测中对待测液的消耗；The design of the dialysis sampling probe greatly reduces the consumption of the liquid to be tested in the test;

实现了葡萄糖的在线检测；Realized the online detection of glucose;

本发明，结构紧凑、加工方便，易于批量化生产。The invention has the advantages of compact structure, convenient processing and easy batch production.

附图说明Description of drawings

图1为本发明的酶注射式葡萄糖传感在线分析仪的系统框图。Fig. 1 is a system block diagram of the enzyme injection glucose sensor on-line analyzer of the present invention.

图2为本发明的酶注射式葡萄糖传感在线分析仪流路结构连接图。Fig. 2 is a connection diagram of the flow path structure of the enzyme injection glucose sensor on-line analyzer of the present invention.

图3为本发明的透析取样探头结构俯视图，。Fig. 3 is a top view of the structure of the dialysis sampling probe of the present invention.

图4为本发明的透析取样探头结构的剖视图。Fig. 4 is a cross-sectional view of the structure of the dialysis sampling probe of the present invention.

图中：1、酶注射式葡萄糖传感在线分析仪流路结构系统，2、三电极检测电路、3、数据采集控制电路，4、液晶屏，5、电脑，6、按键，7、电源，8、酶注射式葡萄糖生物传感器，9、透析取样探头，10、电磁六通阀，11、定量环，12、蠕动泵，13、蠕动泵，14、缓冲液瓶，15、酶液瓶，16、废液瓶，17、蠕动泵，18、固定环，19、外支撑网，20、透析膜，21、内支撑网，22、探头主体，23、探头入口，24、探头出口，25、扩散池。In the figure: 1. Flow path structure system of enzyme injection glucose sensor online analyzer, 2. Three-electrode detection circuit, 3. Data acquisition control circuit, 4. LCD screen, 5. Computer, 6. Button, 7. Power supply, 8. Enzyme injection glucose biosensor, 9. Dialysis sampling probe, 10. Electromagnetic six-way valve, 11. Quantitative loop, 12. Peristaltic pump, 13. Peristaltic pump, 14. Buffer solution bottle, 15. Enzyme solution bottle, 16 , waste liquid bottle, 17, peristaltic pump, 18, fixed ring, 19, outer support net, 20, dialysis membrane, 21, inner support net, 22, probe main body, 23, probe inlet, 24, probe outlet, 25, diffusion pool.

具体实施方式Detailed ways

如图1所示，本发明提供的酶注射式葡萄糖传感在线分析仪系统包括如下部分：酶注射式葡萄糖传感在线分析仪流路结构系统1、三电极检测电路2、数据采集控制电路3、液晶屏4、PC机5、按键6、电源7。As shown in Figure 1, the enzyme injection glucose sensor on-line analyzer system provided by the present invention includes the following parts: enzyme injection glucose sensor on-line analyzer flow path structure system 1, three-electrode detection circuit 2, dataacquisition control circuit 3 ,LCD screen 4,PC machine 5,button 6,power supply 7.

其中，所述酶注射式葡萄糖传感在线分析仪流路结构系统1是仪器的流路结构部分，各部件间通过管路连接，此管路的材料可选不锈钢、聚四氟乙烯、PEEK或硅胶。Wherein, the flow path structure system 1 of the enzyme injection type glucose sensor on-line analyzer is the flow path structure part of the instrument, and the components are connected by pipelines. The material of the pipelines can be stainless steel, polytetrafluoroethylene, PEEK or Silica gel.

如图2所示，酶注射式葡萄糖传感在线分析仪流路结构连接系统1包括如下组件：酶注射式葡萄糖生物传感器8、透析取样探头9、电磁六通阀10、定量环11、蠕动泵12、蠕动泵13、缓冲液瓶14、酶液瓶15、废液瓶16、蠕动泵17。As shown in Figure 2, the flow structure connection system 1 of the enzyme injection glucose sensor on-line analyzer includes the following components: enzymeinjection glucose biosensor 8, dialysis sampling probe 9, electromagnetic six-way valve 10,quantitative loop 11,peristaltic pump 12.Peristaltic pump 13,buffer solution bottle 14,enzyme solution bottle 15,waste liquid bottle 16,peristaltic pump 17.

所述酶注射式葡萄糖生物传感器8的三电极通过电缆连接到三电极检测电路板，实现传感器产生电流的I/V变换及放大；所述酶注射式葡萄糖生物传感器8的被测液入口通过管路与透析取样探头9的探头出口24连接，在蠕动泵17的工作下，透析取样探头9的扩散池中的待测液被引入到被测液池中；所述透析取样探头9的探头入口23通过管路与缓冲液瓶14连接，此管路经过蠕动泵17；The three electrodes of the enzyme-injectedglucose biosensor 8 are connected to the three-electrode detection circuit board through cables to realize the I/V conversion and amplification of the current generated by the sensor; the measured liquid inlet of the enzyme-injectedglucose biosensor 8 passes through the tube The road is connected with theprobe outlet 24 of the dialysis sampling probe 9, and under the work of theperistaltic pump 17, the liquid to be tested in the diffusion cell of the dialysis sampling probe 9 is introduced into the liquid pool to be tested; the probe inlet of the dialysis sampling probe 9 23 is connected withbuffer solution bottle 14 through pipeline, and this pipeline passes throughperistaltic pump 17;

所述电磁六通阀10的第6端口通过管路与缓冲液瓶14连接，此管路经过蠕动泵12；所述电磁六通阀10的第3端口通过管路与酶液瓶15连接，此管路经过蠕动泵13；所述电磁六通阀10的第2端口通过管路与废液瓶16连接；所述电磁六通阀10的第1端口与第4端口相连，之间连接定量环11；所述电磁六通阀10的第5端口通过管路与酶注射式葡萄糖生物传感器8的缓冲液、酶液入口连接；所述酶注射式葡萄糖生物传感器8的缓冲液、酶液出口通过管路与废液瓶16连接；The 6th port of described electromagnetic six-way valve 10 is connected withbuffer bottle 14 by pipeline, and this pipeline passes throughperistaltic pump 12; The 3rd port of described electromagnetic six-way valve 10 is connected with enzymeliquid bottle 15 by pipeline, This pipeline passes through theperistaltic pump 13; the second port of the electromagnetic six-way valve 10 is connected with thewaste liquid bottle 16 through the pipeline; the first port of the electromagnetic six-way valve 10 is connected with the fourth port, and thequantitative Ring 11; the fifth port of the electromagnetic six-way valve 10 is connected to the buffer solution and enzyme solution inlet of the enzymeinjection glucose biosensor 8 through pipelines; the buffer solution and enzyme solution outlet of the enzymeinjection glucose biosensor 8 Connect withwaste liquid bottle 16 by pipeline;

其中，酶注射式葡萄糖生物传感器8已申请发明专利。Among them, the enzymeinjection glucose biosensor 8 has applied for an invention patent.

其中，电磁六通阀10与定量环11构成酶液定量注射装置。Wherein, the electromagnetic six-way valve 10 and thequantitative loop 11 constitute an enzyme liquid quantitative injection device.

其中，透析取样探头9，用于插入待测液中，对待测液进行取样。Wherein, the dialysis sampling probe 9 is used for inserting into the liquid to be tested to sample the liquid to be tested.

其中蠕动泵12、蠕动泵13和蠕动泵17是流量可调、正反转可调的蠕动泵。Wherein theperistaltic pump 12, theperistaltic pump 13 and theperistaltic pump 17 are peristaltic pumps with adjustable flow rate and adjustable forward and reverse rotation.

如图3、图4所示，透析取样探头9用于插入待测液中，对待测液进行取样，其包括如下组件：固定环18、外支撑网19、透析膜20、内支撑网21、探头主体22、探头入口23、探头出口24、扩散池25。As shown in Fig. 3 and Fig. 4, the dialysis sampling probe 9 is used to be inserted into the liquid to be tested, and the liquid to be tested is sampled, and it includes the following components: a fixedring 18, anouter support net 19, adialysis membrane 20, aninner support net 21, Probemain body 22 ,probe inlet 23 ,probe outlet 24 ,diffusion cell 25 .

所述透析取样探头9的探头主体22上部开有探头入口23和探头出口24，探头主体22的底部置有内支撑网21，底部外侧固定有外支撑网19，通过固定环18将内支撑网21和外支撑网19固定在探头主体22底部；所述探头主体22与内支撑网21间构成扩散池25；所述内支撑网21与外支撑网19间固定透析膜20，内支撑网21与外支撑网19对透析膜20起加固作用；所述固定环18外圈带有螺纹，与一端带有螺纹的探头主体22螺纹连接，对外支撑网19、透析膜20、内支撑网21进行固定；所述探头入口23和探头出口24，用于液体的流入与流出。The probemain body 22 top of described dialysis sampling probe 9 hasprobe inlet 23 andprobe outlet 24, and the bottom of probemain body 22 is provided withinner support net 21, andouter support net 19 is fixed on the outside of the bottom, andinner support net 19 is fixed by fixingring 18. 21 and theouter support net 19 are fixed on the bottom of the probemain body 22; thediffusion pool 25 is formed between the probemain body 22 and theinner support net 21; thedialysis membrane 20 is fixed between theinner support net 21 and theouter support net 19, and theinner support net 21 Thedialysis membrane 20 is reinforced with theouter support net 19; the outer ring of the fixingring 18 has threads, and is threadedly connected with theprobe body 22 with threads at one end, and theouter support net 19, thedialysis membrane 20, and the inner support net 21 fixed; theprobe inlet 23 and theprobe outlet 24 are used for inflow and outflow of liquid.

其中，探头主体22材料，可选不锈钢、PEEK等。Wherein, the material of the probemain body 22 may be stainless steel, PEEK or the like.

其中，透析膜20，可选聚碳酸酯膜、聚砜膜等。Wherein, thedialysis membrane 20 can be selected from polycarbonate membrane, polysulfone membrane and the like.

其中，外支撑网19、内支撑网21，可由不锈钢网制成。Wherein, theouter support net 19 and the inner support net 21 can be made of stainless steel net.

其中，通过蠕动泵17正反转，促进待测液中的待测物质加快向扩散池25内扩散。Wherein, through the forward and reverse rotation of theperistaltic pump 17, the substance to be tested in the liquid to be tested is accelerated to diffuse into thediffusion cell 25 .

本发明的酶注射式葡萄糖传感在线分析仪工作过程如下：The working process of the enzyme injection type glucose sensor on-line analyzer of the present invention is as follows:

将透析取样探头9插入待测液中，开启仪器，设置仪器工作参数。如下过程将自动完成，首先，蠕动泵12、蠕动泵13蠕动泵17启动，抽取缓冲液或酶液对电磁六通阀10、定量环11、酶注射式葡萄糖生物传感器8、透析取样探头9进行清洗，清洗完毕，停止3个蠕动泵。然后，低速启动蠕动泵17，令其间歇式的正反转，通过设定扩散时间控制蠕动泵17转动时间。扩散完毕，通过蠕动泵17将扩散进的待测液引入酶注射式葡萄糖生物传感器8的被测液池中，启动酶注射式葡萄糖生物传感器8磁搅拌转子。引入完毕后，软换电磁六通阀10，开启蠕动泵12，将由电磁六通阀10和定量环11组成的酶液定量注射装置所定量的酶液引入到酶注射式葡萄糖生物传感器8的反应池中，在反应池中，葡萄糖氧化酶与待测液中的葡萄糖发生反应，产生传感器电流信号。三电极检测电路、数据采集控制电路系统对此电流信号进行检测、采集和处理，得到与此电流信号对应的葡萄糖浓度数值，此葡萄糖浓度数值可在液晶屏4上进行显示，也可通过串口传给PC机5，进行上位机的处理。Insert the dialysis sampling probe 9 into the liquid to be tested, turn on the instrument, and set the working parameters of the instrument. The following process will be completed automatically. First, theperistaltic pump 12,peristaltic pump 13 andperistaltic pump 17 are started, and the buffer solution or enzyme liquid is extracted to the electromagnetic six-way valve 10, thequantitative loop 11, the enzyme injectiontype glucose biosensor 8, and the dialysis sampling probe 9. Cleaning, after cleaning, stop 3 peristaltic pumps. Then, start theperistaltic pump 17 at a low speed, make it run forward and reverse intermittently, and control the rotation time of theperistaltic pump 17 by setting the diffusion time. After the diffusion is completed, theperistaltic pump 17 introduces the diffused test solution into the test solution pool of the enzymeinjection glucose biosensor 8 , and starts the magnetic stirring rotor of the enzymeinjection glucose biosensor 8 . After the introduction is completed, softly replace the electromagnetic six-way valve 10, turn on theperistaltic pump 12, and introduce the enzyme liquid quantified by the enzyme liquid quantitative injection device composed of the electromagnetic six-way valve 10 and thequantitative loop 11 into the reaction of the enzymeinjection glucose biosensor 8. In the pool, in the reaction pool, the glucose oxidase reacts with the glucose in the liquid to be tested to generate a sensor current signal. The three-electrode detection circuit and the data acquisition control circuit system detect, collect and process the current signal to obtain the glucose concentration value corresponding to the current signal. This glucose concentration value can be displayed on theliquid crystal screen 4 or can be transmitted through the serial port. GivePC machine 5, carry out the processing of upper computer.

Claims (7)

1. enzyme injecting type glucose sensing in-line analyzer, it is characterized in that: it comprises: enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1), three electrode detection circuit (2), data acquiring control circuit (3), liquid crystal display (4), PC (5), button (6), power supply (7), and concrete annexation is:

Described enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1) is connected with data acquiring control circuit (3) with three electrode detection circuit (2), three electrode detection circuit (2) are used for detecting the current signal that enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1) enzyme injecting type glucose biological sensor (8) produces, and data acquiring control circuit (3) is used for providing control signal to enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1); Three electrode detection circuit (2) are connected with data acquiring control circuit (3), and data acquiring control circuit (3) is used to gather the voltage signal of three electrode detection circuit (2) output; Data acquiring control circuit (3) also links to each other with liquid crystal display (4), PC (5), button (6) respectively, liquid crystal display (4) is used for the digital signal of video data acquisition control circuit (3) output, PC (5) can communicate by serial ports and data acquiring control circuit (3), and button (6) is used to be provided with data acquiring control circuit (3) operational factor; Power supply (7) links to each other with three electrode detection circuit (2), data acquiring control circuit (3) and enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1), is they power supplies;

Described enzyme injecting type glucose sensing in-line analyzer flow passage structure connected system (1) comprising: enzyme injecting type glucose biological sensor (8), dialysis sampling probe (9), electromagnetism six-way valve (10), quantitative ring (11), peristaltic pump (12,13,17), damping fluid bottle (14), enzyme liquid bottle (15), waste liquid bottle (16); Concrete annexation is: described dialysis sampling probe (9) comprises set collar (18), outer supporting network (19), dialysis membrane (20), interior supporting network (21), probe body (22), probe inlet (23), probe outlet (24), diffusion cell (25);

Three electrodes of described enzyme injecting type glucose biological sensor (8) are connected to three electrode detection circuit boards by cable, realize that sensor produces the I/V conversion and the amplification of electric current; The test solution inlet of described enzyme injecting type glucose biological sensor (8) is connected with the probe outlet (24) of dialysis sampling probe (9) by pipeline, under the work of peristaltic pump 16, the liquid to be measured in the diffusion cell of dialysis sampling probe (9) is introduced in the tested liquid pool; The probe inlet (23) of described dialysis sampling probe (9) is connected with damping fluid bottle (14) by pipeline, and this pipeline is through peristaltic pump (17);

The 6th port of described electromagnetism six-way valve (10) is connected with damping fluid bottle (14) by pipeline, and this pipeline is through peristaltic pump (12); The 3rd port of described electromagnetism six-way valve (10) is connected with enzyme liquid bottle (15) by pipeline, and this pipeline is through peristaltic pump (13); The 2nd port of described electromagnetism six-way valve (10) is connected with waste liquid bottle (16) by pipeline; The 1st port of described electromagnetism six-way valve (10) links to each other with the 4th port, between connect quantitatively ring (11); The 5th port of described electromagnetism six-way valve 9 is connected with damping fluid, the enzyme liquid inlet of enzyme injecting type glucose biological sensor (8) by pipeline; The damping fluid of described enzyme injecting type glucose biological sensor 8, the outlet of enzyme liquid are connected with waste liquid bottle (16) by pipeline;

Probe body (22) top of described dialysis sampling probe (9) has probe inlet (23) and probe outlet (24), the bottom of probe body (22) is equipped with interior supporting network (21), bottom outside is fixed with outer supporting network (19), by set collar (18) interior supporting network (21) and outer supporting network (19) is fixed on probe body (22) bottom; Constitute diffusion cell (25) between described probe body (22) and interior supporting network (21); Fixing dialysis membrane (20) between supporting network (21) and outer supporting network (19) in described, interior supporting network (21) and outer supporting network (19) are to dialysis membrane (a 20) reinforcement effect; Described set collar (18) outer ring is threaded, and is threaded with the screwed probe body of an end (22), and externally supporting network (19), dialysis membrane (20), interior supporting network (21) are fixed; Described probe inlet (23) and probe outlet (24) are used for the inflow and the outflow of liquid.

2. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: connect the optional stainless steel of the material of this pipeline, teflon, PEEK or silica gel between each parts by pipeline.

3. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: electromagnetism six-way valve 10 constitutes enzyme liquid calibrated shot devices with quantitative ring 11.

4. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: described peristaltic pump (12,13,17) is the peristaltic pump that flow is adjustable, rotating is adjustable.

5. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: the optional stainless steel of described probe body (22) material, PEEK.

6. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: described dialysis membrane (20), optional polycarbonate membrane, polysulfone membrane.

7. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: outer supporting network (19), interior supporting network (21) are made by stainless (steel) wire.

Images