CN101948780B - Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof - Google Patents

Abstract

Translated fromChinese

本发明涉及能克服或消除辣椒疫病的拮抗菌及其生产的微生物有机肥料，属于农业集约化生产技术。本发明分离到了对辣椒连作疫病病原卵菌有显著拮抗作用的拮抗菌(短小芽孢杆菌NJLZ-8)，该拮抗菌与有机肥混合制成微生物有机肥料，肥料中含有1×10⁸个/g以上的拮抗微生物数量、全氮含量为4～5％(90％以上为有机氮)，总氮磷钾养分为6～10％、有机质含量为30～35％。试验表明，这种微生物有机肥料施入土壤后能够使拮抗菌迅速繁殖，在土壤中形成优势群，在辣椒疫病严重的土壤中对辣椒疫病的生物防治率达80％以上。在还没有形成连作障碍的土壤上长期施用本微生物有机肥料能在很大程度上防止辣椒疫病的发生。

The invention relates to antagonistic bacteria capable of overcoming or eliminating pepper blight and the microbial organic fertilizer produced therefrom, which belongs to the agricultural intensive production technology. The present invention has isolated the antagonistic bacteria (Bacillus pumilus NJLZ-8) which has a significant antagonistic effect on the pathogenic oomycete of pepper continuous cropping blight. The antagonistic bacteria are mixed with organic fertilizers to make microbial organic fertilizers, which contain 1×¹⁰⁸ bacteria/g The number of above antagonistic microorganisms, the total nitrogen content is 4-5% (over 90% is organic nitrogen), the total nitrogen, phosphorus and potassium nutrients are 6-10%, and the organic matter content is 30-35%. Tests have shown that this microbial organic fertilizer can make antagonistic bacteria multiply rapidly after being applied to the soil, forming a dominant group in the soil, and the biological control rate of pepper blight can reach more than 80% in the soil with severe pepper blight. Long-term application of the microbial organic fertilizer on the soil where continuous cropping obstacles have not been formed can largely prevent the occurrence of capsicum blight.

Description

The antagonism bacterium and the microorganism organic fertilizer thereof of control continuous cropping capsicum epidemic disease

One, technical field

The present invention relates to a kind of antagonism bacterium and the microorganism organic fertilizer thereof that can prevent and treat the continuous cropping capsicum epidemic disease, belong to the intensive agriculture production technology, be exclusively used in and overcome and eliminate continuous cropping capsicum epidemic disease problem.

Two, background technology

Capsicum originates in the Central and South America torrid zone and subtropical zone, now in China's various places common cultivated, becomes one of maximum vegetable crop of China cultivated area, especially the important export advantage cash crop of earning foreign exchange.Along with the increase of the cultivation time limit, pepper planting district soil-borne disease takes place on a large scale, and particularly the appearance of management modes such as the industrialization of facility production, Focus, mass-producing makes soil-borne disease more become serious.Capsicum epidemic disease is caused by oomycetes pathogenic agent Phytophthora capsici, is the most serious soil-borne disease on capsicum produces, and generation was all arranged from the seedling stage to the fruiting period.Traditional chemical prevention and control method can cause negative impact to the environment and the mankind, if do not prevent and treat the effective ways and the approach of this disease, this will produce very big influence to the capsicum industry of China and the people's life.Our result of study shows that the key issue of succession crop eqpidemic disease is not nutrient deficiency or imbalance, neither the soil secondary salinization problem, but the intoxicating problem of micro-flora in the soil and secondary substance thereof.

On the other hand, the feces of livestock and poultry behind crop material on-site incineration, the large-scale cultivations such as paddy rice, wheat, corn, rape has been thrown aside etc. not only severe contamination everywhere environment, also greatly having wasted can be as the raw material of fertilizer and biological organic fertilizer product; A large amount of nutrient resource (C, N, P, K, S and trace element) runs off outside soil-plant system, has weakened the ability of China's agricultural sustainable development significantly.The nutritive element how will from soil, take away because of crop harvesting is returned in the soil to greatest extent and goes, and unique approach is processed the organic commercial fertilizer material with these solid organic castoffs exactly and is manured into soil.If through the synthetic high-grade organic compost of thermophilic fermentation, as the carrier of function yeast, made microorganism organic fertilizer definite functions will have good application prospects again with these solid organic castoffs.

Three, summary of the invention

1. technical problem

The object of the present invention is to provide a kind of antagonism bacterium and the microorganism organic fertilizer thereof that can prevent and treat the continuous cropping capsicum epidemic disease; The efficient of preventing and treating of continuous cropping capsicum epidemic disease is reached more than 80%; Make a large amount of industrialized agriculture soil and open country capsicum continuous cropping obstacle soil obtain biological prosthetic, guarantee agriculture the shaping up that intensify.

2. technical scheme

One strain can overcome or eliminate the antagonism bacterium NJLZ-8 of continuous cropping capsicum epidemic disease; This mikrobe belongs to the bacillus pumilus (Bacillus pumilus) of Gram-positive, is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, address: Datun Road, Chaoyang District, Beijing City on July 9th, 2009; Institute of Microorganism, Academia Sinica; Culture presevation number is CGMCC NO.3182, and main biological characteristics is: main biological characteristics is Gram-positive (G+), produces gemma.Cell is shaft-like, aerobic or amphimicrobian growth, and catalase is positive, oxidase negative; The starch hydrolysis is negative, the V.P. reacting positive, and methyl red test is negative, and glucose fermentation produces not aerogenesis of acid; Unfermentable wood sugar, L-arabinose, N.F,USP MANNITOL can not utilize Citrate trianion, the nitrate reductase enzyme positive; Gelatin liquefaction positive, lecithinase is negative, not decomposition of cellulose.

The microorganism organic fertilizer of preventing and kill off the continuous cropping capsicum epidemic disease with above-mentioned antagonism bacterium produces contains 1 * 10 in this fertilizer⁸The antagonism bacterium NJLZ-8 that individual/g is above, full nitrogen quality is that 4～5% (being organonitrogen 90% or more), total nitrogen phosphorus potassium nutrition are 6～10%, organic content is 30～35% than content.

The working method of said microorganism organic fertilizer comprises:

1) antagonism bacterium NJLZ-8 is inoculated into the PDA nutrient solution, carries out liquid fermenting production, the condition of fermentative prodn is: pH growth scope nature; 30～35 ℃ of culture temperature scopes; Stirring velocity is 180～300 rev/mins, and the fermentation middle and later periods forms gemma, makes to contain bacterium or gemma amount>=1 * 10 in the fermented liquid¹⁰Individual/ml.Used PDA nutrient solution compound method does, is example with preparation 1L substratum: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling; Add the 20g common sucrose in the filtrating after filtering; Be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min.

2) with antagonism bacterium NJLZ-8 fermented liquid respectively with become thoroughly decomposed after pig manure and dish dregs of rice mikrobe enzymolysis mixture carry out solid fermentation; Add antimicrobial fluid 50L short of money in the SOLID ORGANIC material per ton; Turning every day is 1 time in the solid fermentation process; Make the solid fermentation temperature be no more than 50 ℃, ferment and finish after 5 days, make antagonism bacterium NJLZ-8 content reach 1 * 10⁸Individual/more than the g; Obtain pig manure microbial organic fertilizer and dish dregs of rice enzymolysis mixture microbial organic fertilizer, the pig manure germination index after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content>=35%; The organonitrogen quality is 1.2-2% than content, water cut mass ratio 25-30%.The mikrobe enzymolysis preparation method of mixture of its Chinese food dregs of rice does, is that germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid of CGMCC NO.1543 joins in the dish dregs of rice raw material with preserving number, regulates water cut 55-65% and pH 6.0-7.5; Carry out open solid fermentation, treat to begin immediately when leavening temperature rises to 50 ℃ turning, later turning every day; Make the heap temperature maintain all the time 35-50 ℃ totally 5 days, after solid fermentation began, the pH of stacking yard can constantly rise; During each turning with containing acid solution body adjusting water cut and pH; Make them maintain 55-65% and 6.0-7.0 respectively, spray with containing the acid solution body again when fermentation stops, be adjusted into about 5.0 until stacking yard pH; Carry out cryodrying or natural airing again, be the mikrobe enzymolysis mixture of the dish dregs of rice.

3) then with the pig manure microbial organic fertilizer of solid fermentation and 60: 40 by volume ratio thorough mixing after-ripening of dish dregs of rice enzymolysis mixture microbial organic fertilizer 2 days; Turning is 2 times in the after-ripening process; With the water cut of after-ripening thing cryodrying to 30%, packing is dispatched from the factory and is the microbial organic fertilizer that can prevent and treat the continuous cropping capsicum epidemic disease.

Said microorganism organic fertilizer can be exclusively used in control continuous cropping capsicum epidemic disease.

3. beneficial effect

The present invention relates to a kind of microorganism organic fertilizer and working method thereof that can overcome or eliminate the continuous cropping capsicum epidemic disease; Utilize pig manure and dish dregs of rice mikrobe enzymolysis aminoacid mixture; Process microorganism organic fertilizer again with after antimicrobial fluid short of money is mixed, its product is compared with product in the market has following advantage:

1) contain the efficient bacterial strain of specificity (bacillus pumilus NJLZ-8) that suppresses the growth of capsicum epidemic disease cause of disease oomycetes in the fertilizer product, it suppresses the effect highly significant, and the content of this efficient bacterial strain reaches 1 * 10 in the fertilizer⁸Individual/more than the g, ability formation advantage crowd after being manured into soil, and under in soil, surviving.

2) contain rich soluble organic carbon and organonitrogen in the fertilizer product, can very superior growing environment be provided, greatly improved the ability that function is grown and brought into play to antagonism bacterium deciding in soil to function yeast.

3) product organophosphorus of the present invention and organonitrogen content are high especially, all reach 4%.One of this products material is a pig manure, and total phosphorous is very high, and biological effectiveness is high especially, in this microorganism organic fertilizer product, need not to add any phosphatization fertilizer that contains, and uses to make behind this product crop spend phosphorus in seedling stage plain critical porion smoothly.By contrast, it is relatively poor to contain the phosphatization fertilizer artifact validity that is manured into soil; Organonitrogen can reach more than 4% (most of be amino acid and micromolecule polypeptide) in this product, has guaranteed the continual and steady supply of fertilizer nitrogen, make plant plant growth and anti-adversity ability strengthens greatly.

4) result of study shows, after using this product on the soil of continuous cropping capsicum, can significantly reduce the sickness rate of capsicum epidemic disease, and the biological control rate reaches more than 80%, and plant grow fine (Fig. 2).The soil of this fertilizer of continuous administration is higher to the biological control rate of continuous cropping capsicum epidemic disease.

Four, description of drawings

The dull and stereotyped antagonistic effect of Fig. 1 antagonistic strain NJLZ-8

The potted plant effect of Fig. 2 is annotated: the conventional fertilizer 3.CK+ of 1.CK (continuous cropping capsicum epidemic disease soil is arranged) 2.CK+ antagonism bacterium NJLZ-8 bacterium liquid 4.CK+ antagonism bacterium NJLZ-8 is through the fertilizer (fertilizer product of the present invention) of Secondary Fermentation

Five, embodiment

(1) separation of bacterial strain and evaluation

Gather the plant that capsicum epidemic disease has taken place, adopt the mould selective medium of epidemic disease to separate and obtain pathogenic bacteria, and verify that it is pathogenic from the strong position, boundary of the disease of the stem of morbidity capsicum.Be indicator with the capsicum epidemic disease pathogenic bacteria then, separate the antagonism bacterium in the healthy plant rhizosphere soil from the piece of continuous cropping capsicum epidemic disease morbidity field.Sieve efficient antagonistic strain again through pot experiment then, finally obtain antagonism bacterium NJLZ-8 and identify that bacterial classification is-70 ℃ of preservations in 30% glycerine.Dull and stereotyped face-off test-results finds that antagonism bacterium NJLZ-8 reaches 68% (like Fig. 1) to the inhibiting rate of pathogenic bacteria.

Antagonism bacterium NJLZ-8 belongs to bacillus pumilus (Bacillus pumilus), and its main biological characteristics is: Gram-positive (G⁺), produce gemma.Cell is shaft-like, aerobic or amphimicrobian growth, and catalase is positive, oxidase negative; The starch hydrolysis is negative, the V.P. reacting positive, and methyl red test is negative, and glucose fermentation produces not aerogenesis of acid; Unfermentable wood sugar, L-arabinose, N.F,USP MANNITOL can not utilize Citrate trianion, the nitrate reductase enzyme positive; Gelatin liquefaction positive, lecithinase is negative, not decomposition of cellulose.

(2) microbial inoculum production

1) antagonism bacterium NJLZ-8 is inoculated into the PDA nutrient solution, carries out liquid fermenting production, the condition of fermentative prodn is: pH growth scope nature; 30～35 ℃ of culture temperature scopes; Stirring velocity is 180～300 rev/mins, and the fermentation middle and later periods forms gemma, makes to contain bacterium or gemma amount>=1 * 10 in the fermented liquid¹⁰Individual/ml.Used PDA nutrient solution compound method does, is example with preparation 1L substratum: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling; Add the 20g common sucrose in the filtrating after filtering; Be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min.

2) with antagonism bacterium NJLZ-8 fermented liquid respectively with become thoroughly decomposed after pig manure and dish dregs of rice mikrobe enzymolysis mixture carry out solid fermentation respectively; Add antimicrobial fluid 50L short of money in the SOLID ORGANIC material per ton; Turning every day is 1 time in the solid fermentation process; Make the solid fermentation temperature be no more than 50 ℃, ferment and finish after 5 days, make the antagonism bacterial content reach 1 * 10⁸Individual/as more than the g, to obtain the pig manure microbial organic fertilizer and the dish dregs of rice enzymolysis mixture microbial organic fertilizer of solid fermentation.Pig manure germination index after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content >=35%, and the organonitrogen quality is 1.2-2% than content, water cut mass ratio 25-30%.

The mikrobe enzymolysis preparation method of mixture of its Chinese food dregs of rice is (public, as to see patent of invention ZL200610086126.0, a kind of organism preparation method of agricultural amino acid and fertilizer product thereof); With preserving number is that germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid of CGMCC NO.1543 joins in the dish dregs of rice raw material, regulates water cut 55-65% and pH 6.0-7.5, carries out open solid fermentation; Treat to begin immediately when leavening temperature rises to 50 ℃ turning, later turning every day, make the heap temperature maintain all the time 35-50 ℃ totally 5 days; After solid fermentation begins; The pH of stacking yard can constantly rise, and with containing acid solution body adjusting water cut and pH, makes them maintain 55-65% and 6.0-7.0 respectively during each turning; When stopping, fermentation sprays with containing the acid solution body again; Be adjusted into about 5.0 until stacking yard pH, carry out cryodrying or natural airing again, be the mikrobe enzymolysis mixture (containing amino acid whose mixing material) of the dish dregs of rice.

3) then with pig manure microbial organic fertilizer and 60: 40 by volume ratio thorough mixing after-ripening of dish dregs of rice enzymolysis mixture microbial organic fertilizer 2 days; Turning is 2 times in the after-ripening process; With the water cut of after-ripening thing cryodrying to 30%, packing is dispatched from the factory and is the microbial organic fertilizer product that can prevent and treat the continuous cropping capsicum epidemic disease.

The above-mentioned continuous cropping capsicum epidemic disease microorganism organic fertilizer of preventing and kill off contains 1 * 10 in this fertilizer product⁸The above antagonism bacterium NJLZ-8 of individual/g, full nitrogen quality are that 4～5% (being organonitrogen 90% or more), total nitrogen phosphorus potassium nutrition mass ratio are 6～10%, the quality of organic matter ratio content is 30～35% than content.

(3) pot experiment

In the pot experiment, capsicum variety is Soviet Union green pepper No. five, when adopting healthy paddy rice seedlings and soil to 6～8 true leaves, transplants to little plastic tub.The serious soil of continuous cropping capsicum epidemic disease picks up from the Funan County, Anhui.The serious soil 3kg of capsicum epidemic disease is made in every basin load, and antagonism bacterium NJLZ-8 bacterium liquid is handled with 10⁸Cfu/g soil is evenly admixed in the continuous cropping capsicum epidemic disease soil, and fertilizer product of the present invention is evenly admixed in the sick soil with 2% usage quantity (w/w).Each handles 3 groups of repetitions, and every group is repeated 6 strain capsicums.Keep respectively handling humidity in the soil, temperature unanimity during cultivation.After transplanting 35d, the statistics incidence is measured each item index.

The result shows that the sickness rate of continuous cropping soil is 88%, and survival plant growing way is short and small, poor growth; The sickness rate that inoculation antagonism bacterium NJLZ-8 bacterium liquid is handled in the continuous cropping soil is 25%, and control rate reaches 72%, and using the sickness rate that fertilizer product of the present invention handles in the continuous cropping soil is 16%, and control rate reaches 82% (table 1), and plant grow fine (Fig. 2).Explain that this product can effectively suppress the generation of capsicum epidemic disease, improve the control effect of the control of capsicum epidemic disease.

The yield of hot pepper of control treatment is merely 4gpot in the continuous cropping soil^-1, and use output that fertilizer product of the present invention handles in the continuous cropping soil for being 43.92gpot^-1, raising the output reaches 9.95 times.The capsicum overground part fresh weight of continuous cropping soil is the 19.60g/ strain, and dry weight is the 4.10g/ strain, is the 43.92g/ strain and use the capsicum fresh weight that fertilizer product of the present invention handles in the continuous cropping soil, and dry weight is 12.91g/ strain (table 2), the living weight significant difference; Use fertilizer product of the present invention in the continuous cropping soil and handle than control treatment, blade Net Photosynthetic Rate, transpiration rate significantly increase (table 3), help the accumulation and the storage of plant photosynthesis product, thereby improve yield of hot pepper and living weight.These results show that this product can also reduce or eliminate pathogenic bacteria to the restraining effect that chili growth produces except the generation that can effectively reduce capsicum epidemic disease, promote the growth of capsicum significantly.

Table 1 pot experiment capsicum epidemic disease sickness rate and control effect

Table 2 pot experiment yield of hot pepper and living weight

The photosynthetic index of table 3 pot experiment capsicum functional leaf

Patent of the present invention is set about from this key issue of micro-flora of continuous cropping soil, develops a kind of microorganism organic fertilizer product that can significantly reduce continuous cropping capsicum epidemic disease sickness rate, and control rate reaches more than 80%.The mechanism of action of this fertilizer product be function yeast that this fertilizer product provides and effectively carbon and effective nitrogenous source to the cultivation of continuous cropping obstacle soil useful microbe fauna good condition is provided, make it to recover rapidly ecology and the food chain thereof of indigenous useful microbe and external source function yeast mikrobe; On the other hand, contain the organonitrogen and the organophosphorus of high level in this fertilizer product, these nutritive substances are very favourable to the growth of crop in the continuous cropping obstacle soil, improved upright seedling rate and the anti-adversity ability of crop greatly.

Claims (6)

Translated fromChinese

1.一种能防治连作辣椒疫病的拮抗菌NJLZ-8，属于短小芽孢杆菌(Bacilluspumilus)，于2009年7月9日保藏于中国微生物菌种保藏管理委员会普通微生物中心，菌种保藏号为CGMCC NO.3182；1. An antagonistic bacteria NJLZ-8 capable of preventing and treating continuous cropping pepper blight, belonging to Bacillus pumilus, was deposited in the General Microorganism Center of China Microbiological Culture Collection Management Committee on July 9, 2009, and the strain preservation number is CGMCC NO.3182;NJLZ-8的主要生物学特性为：革兰氏染色阳性G⁺，产芽孢，细胞为杆状，好氧或兼性厌氧生长，接触酶阳性，氧化酶阴性，淀粉水解阴性，V.P.反应阳性，甲基红试验阴性，发酵葡萄糖产酸不产气，不能发酵木糖、L-阿拉伯糖、甘露醇，不能利用柠檬酸盐，硝酸还原酶阳性，明胶水解阳性，卵磷脂酶阴性，不分解纤维素。The main biological characteristics of NJLZ-8 are: Gram stain positive G⁺ , sporulation, rod-shaped cells, aerobic or facultative anaerobic growth, catalase positive, oxidase negative, starch hydrolysis negative, VP reaction positive , negative methyl red test, fermenting glucose to produce acid but not gas, unable to ferment xylose, L-arabinose, mannitol, unable to utilize citrate, positive for nitrate reductase, positive for hydrolysis of gelatin, negative for lecithinase, not decomposed cellulose.2.用权利要求1所述拮抗菌生产的防治连作辣椒疫病的微生物有机肥料，其特征在于：该肥料中含有1×10⁸个/g以上的拮抗菌NJLZ-8，全氮质量比含量为4～5％，全氮中质量比90％以上为有机氮，总氮磷钾养分质量比为6～10％，有机质质量比含量为30～35％。2. the microbial organic fertilizer of the prevention and treatment of continuous cropping capsicum blight with the described antagonistic bacteria production of claim 1, it is characterized in that: contain 1 *¹⁰ in this fertilizer More than antagonistic bacteria NJLZ-8 of /g, total nitrogen mass ratio content is 4-5%, more than 90% of the mass ratio of total nitrogen is organic nitrogen, the mass ratio of total nitrogen, phosphorus and potassium nutrients is 6-10%, and the mass ratio of organic matter is 30-35%.3.权利要求2所述微生物有机肥料的生产方法，包括：3. the production method of the described microbial organic fertilizer of claim 2, comprising:1)将权利要求1所述的拮抗菌NJLZ-8接种到PDA培养液，进行液体发酵生产，发酵生产的条件为：培养温度范围30～35℃，搅拌速度为180～300转/分钟，发酵中后期形成芽孢，使发酵液中含菌或芽孢量≥1×10¹⁰个/ml；1) the antagonistic bacteria NJLZ-8 described in claim 1 is inoculated into PDA culture fluid, carries out liquid fermentation production, the condition of fermentation production is: culture temperature range 30～35 ℃, stirring speed is 180～300 rev/min, fermentation Spores are formed in the middle and later stages, so that the bacteria or spores in the fermentation broth are ≥1×¹⁰¹⁰ /ml;2)将拮抗菌NJLZ-8发酵液分别与腐熟后的猪粪堆肥和菜粕微生物酶解混合物进行固体发酵，每吨固体有机物料中加入NJLZ-8发酵液50L，固体发酵过程中每天翻堆1次，使固体发酵温度不超过50℃，发酵5天后结束，使拮抗菌NJLZ-8含量达到1×10⁸个/g以上，获得猪粪堆肥微生物有机肥和菜粕酶解混合物微生物有机肥；其中菜粕的微生物酶解混合物制备方法为，将保藏号为CGMCC NO.1543的嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)菌株37-1发酵液加入到菜粕原料中，调节含水量55-65％和pH 6.0-7.5，进行开放式固体发酵，待发酵温度上升至50℃时立即开始翻堆，以后每天翻堆，使堆温始终维持在35-50℃共5天，固体发酵开始后，堆物的pH会不断上升，每次翻堆时用含酸液体调节含水量和pH，使它们分别维持在55-65％和6.0-7.0，发酵终止时再用含酸液体进行喷洒，直至堆物pH调整为5.0，再进行低温干燥或自然凉干，即为菜粕的微生物酶解混合物；2) The antagonistic bacteria NJLZ-8 fermented liquid was fermented with the decomposed pig manure compost and rapeseed meal microbial enzymatic hydrolysis mixture respectively, and 50L of NJLZ-8 fermented liquid was added to each ton of solid organic material, and the compost was turned every day during the solid fermentation process 1 time, make the solid fermentation temperature not exceed 50°C, finish the fermentation after 5 days, make the content of antagonistic bacteria NJLZ-8 reach more than 1×¹⁰⁸ /g, and obtain pig manure composting microbial organic fertilizer and rapeseed meal enzymatic hydrolysis mixture microbial organic fertilizer wherein the preparation method of the microbial enzymatic hydrolysis mixture of rapeseed meal is that the fermentation liquid of Stenotrophomonas maltophilia (Stenotrophomonas maltophilia) bacterial strain 37-1 whose preservation number is CGMCC NO.1543 is added in the raw material of rapeseed meal, and the water content is adjusted to 55 -65% and pH 6.0-7.5, carry out open-type solid fermentation, start turning the compost immediately when the fermentation temperature rises to 50°C, and then turn the compost every day to keep the compost temperature at 35-50°C for 5 days, and the solid fermentation begins Afterwards, the pH of the heap will continue to rise, and each time the pile is turned over, the water content and pH are adjusted with an acidic liquid to maintain them at 55-65% and 6.0-7.0, respectively, and then sprayed with an acidic liquid when the fermentation is terminated. Until the pH of the heap is adjusted to 5.0, then low-temperature drying or natural drying is carried out, which is the microbial enzymatic hydrolysis mixture of rapeseed meal;3)然后将固体发酵的猪粪堆肥微生物有机肥和菜粕酶解混合物微生物有机肥按体积比60∶40的比例充分混合后熟2天，后熟过程中翻堆2次，将后熟物低温干燥至30％的含水量，包装出厂即为能防除连作辣椒疫病的微生物有机肥。3) Then fully mix the solid fermented pig manure composting microbial organic fertilizer and the rapeseed meal enzymatic hydrolysis mixture microbial organic fertilizer according to the ratio of 60:40 by volume and ripen for 2 days, turn the pile twice during the post-ripening process, and put the post-cooked Drying at low temperature to 30% water content, packaged and delivered is a microbial organic fertilizer that can prevent continuous cropping pepper blight.4.根据权利要求3中所述微生物有机肥料的生产方法，其中步骤1)所用PDA培养液配制方法为：用200g土豆削皮后切成小块放到水里煮，沸腾后煮30min，经过滤后滤液中加20g普通蔗糖，定容至1000ml，pH值调至7.2-7.4，121℃灭菌20min。4. according to the production method of microbial organic fertilizer described in claim 3, wherein step 1) used PDA nutrient solution preparation method is: after peeling with 200g potato, be cut into fritters and put into water and boil, after boiling, boil 30min, through After filtering, add 20 g of ordinary sucrose to the filtrate, adjust the volume to 1000 ml, adjust the pH value to 7.2-7.4, and sterilize at 121 ° C for 20 min.5.根据权利要求3或4所述微生物有机肥料的生产方法，其中步骤2)所用腐熟后的猪粪堆肥发芽指数为98％以上，有机肥质量比含量≥35％，有机氮质量比含量为1.2-2％，含水量质量比25-30％。5. according to the production method of the described microbial organic fertilizer of claim 3 or 4, wherein step 2) the pig manure composting germination index after being decomposed used is more than 98%, organic fertilizer mass ratio content >=35%, organic nitrogen mass ratio content is 1.2-2%, water content mass ratio 25-30%.6.权利要求2所述微生物有机肥料在防治连作辣椒疫病中的应用。6. the application of the microbial organic fertilizer described in claim 2 in the prevention and treatment of continuous cropping capsicum blight.