CN101824384A

Movatterモバイル変換

Info

Publication number: CN101824384A
Application number: CN 201010146768
Authority: CN
Inventors: 郑江; 王博
Original assignee: Jimei University
Current assignee: Jimei University
Priority date: 2010-04-08
Filing date: 2010-04-08
Publication date: 2010-09-08

Abstract

本发明公开了一种利用半滑舌鳎养殖废水培养金藻的方法，它包括将半滑舌鳎养殖废水沉淀或过滤后配成混合培养液、培养湛江等鞭金藻等步骤。由于本发明采用了以半滑舌鳎养殖废水为主体的培养液，从而大幅度降低了培养成本，并在获得该微藻的同时，实现对养殖废水的净化，并降低碳排放。此外，在改善养殖水质的同时，也获得了具有较高经济价值的湛江等鞭金藻，具有较好的经济和社会效益。

The invention discloses a method for cultivating golden algae by using tongue sole culture wastewater, which comprises the steps of preparing a mixed culture solution after sedimenting or filtering the tongue sole culture wastewater, cultivating dinoflagellates such as Zhanjiang et al. Since the present invention adopts the culture solution mainly of tongue sole culture wastewater, the culture cost is greatly reduced, and while the microalgae is obtained, the culture wastewater can be purified and carbon emissions can be reduced. In addition, while improving the water quality of aquaculture, dinoflagellates such as Zhanjiang with high economic value are also obtained, which has good economic and social benefits.

Description

Translated fromChinese

利用半滑舌鳎养殖废水培养金藻的方法Method for Cultivating Golden Algae Using Wastewater from Semi-smooth Tongue Sole Breeding

技术领域technical field

本发明涉及利用一种水产养殖废水培养金藻的技术，尤其涉及一种利用半滑舌鳎养殖废水培养金藻的方法。The invention relates to a technique for cultivating golden algae by using aquaculture wastewater, in particular to a method for cultivating golden algae by using the wastewater of tongue sole aquaculture.

背景技术Background technique

水产养殖废水，富含氮、磷等营养元素和有机质，它的超标和过度排放是造成水体富营养化和周边水域环境污染的一个重要因素，尽管目前已开发出了不少针对养殖废水的处理技术和设备，但由于相关技术还不够完善和成熟、以及较高的运行成本和能耗，实际应用效果并不理想。Aquaculture wastewater is rich in nutrients such as nitrogen and phosphorus and organic matter. Its excessive discharge is an important factor causing eutrophication of water bodies and environmental pollution of surrounding waters. Although many treatments for aquaculture wastewater have been developed technology and equipment, but because the relevant technology is not perfect and mature enough, as well as high operating costs and energy consumption, the actual application effect is not ideal.

湛江等鞭金藻(Isochrysis zhan jiangensis)是一种重要的被广泛利用的海洋单胞藻，是双壳类软体动物和海参类幼虫的优质饵料。因此，对湛江等鞭金藻饵料的培养是水产动物育苗系统工作的重要环节之一，目前实验室和生产上主要采用人工配制的含氮、磷等元素丰富的f/2培养液来进行培养。由于培养液中营养元素和微量元素众多，不仅配制麻烦，也增加了培养成本。Isochrysis zhan jiangensis (Isochrysis zhan jiangensis) is an important and widely used marine unicellular algae, which is a high-quality bait for bivalve molluscs and sea cucumber larvae. Therefore, the cultivation of Isoflagellate Zhanjiang bait is one of the important links in the work of the aquatic animal seedling system. At present, the artificially prepared f/2 culture solution rich in nitrogen, phosphorus and other elements is mainly used for cultivation in the laboratory and production. . Because there are many nutrients and trace elements in the culture solution, not only the preparation is troublesome, but also the culture cost is increased.

研究发现，金藻等微藻能够吸收、消耗水体中的氮、磷等营养元素和有机质，并能吸收利用水体和空气中的二氧化碳进行光合作用。因此，利用养殖废水来培养金藻等经济微藻，不仅能净化养殖废水，降低碳排放，而且收获的微藻还可用于水产动物的饵料或饲料的开发，这既降低了养殖废水的处理费用，也为金藻等经济微藻的生产提供了一条低成本的开发途径，能起到一举两得的作用。Studies have found that microalgae such as golden algae can absorb and consume nutrients such as nitrogen and phosphorus and organic matter in water bodies, and can absorb and utilize carbon dioxide in water bodies and air for photosynthesis. Therefore, the use of aquaculture wastewater to cultivate economic microalgae such as golden algae can not only purify the aquaculture wastewater and reduce carbon emissions, but also the harvested microalgae can also be used for the development of bait or feed for aquatic animals, which not only reduces the treatment cost of aquaculture wastewater , also provides a low-cost development approach for the production of economical microalgae such as Chrysogenum algae, and can play the role of killing two birds with one stone.

发明内容Contents of the invention

本发明的目的在于提供一种开发成本较低的利用半滑舌鳎养殖废水培养金藻的方法。The purpose of the present invention is to provide a method for cultivating golden algae using tongue sole culture wastewater with low development cost.

为实现上述目的，本发明的技术方案如下：To achieve the above object, the technical scheme of the present invention is as follows:

本发明是一种利用半滑舌鳎养殖废水培养金藻的方法，它包括以下步骤：The present invention is a kind of method utilizing half-smooth tongue sole culture waste water to cultivate golden algae, and it comprises the following steps:

(1)将半滑舌鳎养殖废水沉淀或过滤后配成混合培养液：在半滑舌鳎养殖废水加入下述含量的物质，(1) Precipitate or filter the half-smooth tongue sole culture wastewater to make a mixed culture solution: add the following substances to the half-smooth tongue sole culture wastewater,

硝酸钠含量为0-15mg/L；Sodium nitrate content is 0-15mg/L;

磷酸二氢钠含量为0-1mg/L；Sodium dihydrogen phosphate content is 0-1mg/L;

柠檬酸铁含量为0-1mg/L；Ferric citrate content is 0-1mg/L;

碳酸氢钠含量为0-0.5g/L；Sodium bicarbonate content is 0-0.5g/L;

维生素B₁含量为0-100ug/L；Vitamin_B1 content is 0-100ug/L;

维生素B₁₂含量为0-100ng/L；The content of vitamin B₁₂ is 0-100ng/L;

(2)培养湛江等鞭金藻：将配成的混合培养液，在0-30℃温度下，pH4-8，光照500LX-10000LX以上，湛江等鞭金藻的初始接种密度为0.002-2mg/mL以上进行培养5-30天即可获得大量湛江等鞭金藻。(2) Cultivate Isochrysis Zhanjiang: Prepare the mixed culture solution at a temperature of 0-30°C, pH 4-8, and light above 500LX-10000LX. The initial inoculation density of Isochrysis Zhanjiang is 0.002-2mg/ mL or more can be cultivated for 5-30 days to obtain a large amount of Isoflagellates zhanjiang.

与现有培养技术相比，本发明的突出优点在于采用了以半滑舌鳎养殖废水为主体的培养液，从而大幅度降低了培养成本，并在获得该微藻的同时，实现对养殖废水的净化，降低了碳排放。Compared with the existing culture technology, the outstanding advantage of the present invention is that it adopts the culture liquid mainly containing the culture wastewater of tongue sole, thereby greatly reducing the culture cost, and realizes the purification of the culture wastewater while obtaining the microalgae, Reduced carbon emissions.

按照雷衍之主编的《养殖水环境化学实验》中的方法，对养殖废水中的氮、磷等指标进行测定(硝态氮采用锌-镉还原法，氨态氮采用靛酚蓝法，亚硝态氮采用重氮-偶氮光度法，活性磷采用钼-锑-抗法)，结果显示，随着湛江等鞭金藻的生长(如图1所示)，水体中的硝态氮、亚硝态氮、活性磷等营养元素的含量大幅下降(如图2所示)，经过11天的培养，金藻对活性磷的清除率达到78.0％，对硝态氮的清除率为84.7％，对亚硝态氮的清除率为62.3％，而对氨态氮则在第2天就完全清除，从而较好地降低了养殖水体的富营养化水平。According to the method in "Chemistry Experiment of Aquaculture Water Environment" edited by Lei Yanzhi, the nitrogen, phosphorus and other indicators in the aquaculture wastewater were measured (the zinc-cadmium reduction method was used for nitrate nitrogen, the indophenol blue method was used for ammonia nitrogen, and the indophenol blue method was used for sub- Nitrate nitrogen adopts diazo-azo spectrophotometry, and active phosphorus adopts molybdenum-antimony-resistance method), the results show that along with the growth of Zhanjiang dinoflagellates (as shown in Figure 1), the nitrate nitrogen, The content of nutrients such as nitrite nitrogen and active phosphorus decreased significantly (as shown in Figure 2). After 11 days of cultivation, the scavenging rate of golden algae to active phosphorus reached 78.0%, and the scavenging rate to nitrate nitrogen was 84.7%. , the scavenging rate of nitrite nitrogen was 62.3%, and the ammoniacal nitrogen was completely removed on the second day, thus reducing the eutrophication level of the aquaculture water better.

本发明利用半滑舌鳎养殖废水来培养湛江等鞭金藻，在改善养殖水质的同时，也获得了具有较高经济价值的湛江等鞭金藻，具有较好的经济和社会效益。The invention utilizes semi-smooth sole aquaculture wastewater to cultivate Isoflagellate Zhanjiang, while improving the culture water quality, also obtains Isoflagellate Zhanjiang with high economic value, and has better economic and social benefits.

下面结合附图和具体实施例对本发明作进一步的说明。The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

附图说明Description of drawings

图1是本发明的湛江等鞭金藻在半滑舌鳎养殖废水中的生长曲线图；Fig. 1 is the growth curve figure of Isochrysis zhanjiang of the present invention in half-smooth tongue sole culture wastewater;

图2是本发明的湛江等鞭金藻对半滑舌鳎养殖废水的净化曲线图。Fig. 2 is a graph showing the purification curve of Isochrysis zhanjiang of the present invention on tongue sole culture wastewater.

具体实施方式Detailed ways

实施例1Example 1

将半滑舌鳎养殖废水1L沉淀或过滤后，加入硝酸钠7.5mg，磷酸二氢钠0.5mg，柠檬酸铁0.5mg，碳酸氢钠含量为0.25g，维生素B₁50ug，维生素B₁₂50ng，再加入湛江等鞭金藻藻种10mg，在20℃温度下，pH7，光照5000LX下培养10天即可获得大量湛江等鞭金藻。After precipitating or filtering 1L of tongue sole breeding wastewater, add 7.5mg of sodium nitrate, 0.5mg of sodium dihydrogen phosphate, 0.5mg of ferric citrate, 0.25g of sodium bicarbonate, 50ug of vitamin_B1 , 50ng of vitamin_B12 , and then add Zhanjiang A large amount of Isochrysis Zhanjiang can be obtained by culturing 10 mg of Isochrysis algae species at a temperature of 20°C, pH 7, and light of 5000 LX for 10 days.

实施例2Example 2

将半滑舌鳎养殖废水800mL沉淀或过滤后，加入硝酸钠0mg，磷酸二氢钠0mg，柠檬酸铁0mg，碳酸氢钠含量为0g，维生素B₁10ug，维生素B₁₂10ng，再加入湛江等鞭金藻藻种1.6mg，在0℃温度下，pH4，光照500LX下培养5天即可获得大量湛江等鞭金藻。After precipitating or filtering 800mL of tongue sole breeding wastewater, add 0mg of sodium nitrate, 0mg of sodium dihydrogen phosphate, 0mg of iron citrate, 0g of sodium bicarbonate, 10ug of vitamin B₁ , 10ng of vitamin B₁₂ , and then add dinoflagellate algae such as Zhanjiang 1.6 mg, at 0°C,pH 4, and light 500LX for 5 days, a large amount of Isochrysis zhanjiang can be obtained.

实施例3Example 3

将半滑舌鳎养殖废水1L沉淀或过滤后，加入硝酸钠15mg，磷酸二氢钠1mg，柠檬酸铁1mg，碳酸氢钠含量为0.5g，维生素B₁100ug，维生素B₁₂100ng，再加入湛江等鞭金藻藻种2g，在30℃温度下，pH8，光照10000LX下培养30天即获得大量湛江等鞭金藻。After precipitating or filtering 1L of tongue sole breeding wastewater, add 15mg of sodium nitrate, 1mg of sodium dihydrogen phosphate, 1mg of iron citrate, 0.5g of sodium bicarbonate, 100ug of vitamin B₁ , 100ng of vitamin B₁₂ , and then add dinoflagellate algae such as Zhanjiang Seed 2g, culture at 30°C,pH 8, and light 10000LX for 30 days to obtain a large number of Isochrysis zhanjiang.

实施例4Example 4

将半滑舌鳎养殖废水1L沉淀或过滤后，加入硝酸钠8mg，磷酸二氢钠0.4mg，柠檬酸铁0.6mg，碳酸氢钠含量为0.3g，维生素B₁60ug，维生素B₁₂60ng，再加入湛江等鞭金藻藻种20mg，在25℃温度下，pH6，光照6000LX下培养15天即获得大量湛江等鞭金藻。After precipitating or filtering 1L of semi-smooth tongue sole breeding wastewater, add 8mg of sodium nitrate, 0.4mg of sodium dihydrogen phosphate, 0.6mg of iron citrate, 0.3g of sodium bicarbonate, 60ug of vitamin_B1 , 60ng of vitamin_B12 , and then add Zhanjiang, etc. 20 mg of dinoflagellate algae species, cultured for 15 days at a temperature of 25°C,pH 6, and light of 6000 LX, can obtain a large number of dinoflagellates Zhanjiang.

Claims

1. method of utilizing cynoglossus semilaevis cultivation waste water to cultivate chrysophyceae, it is characterized in that: it may further comprise the steps:

(1) will cynoglossus semilaevis cultivation waste water precipitation or filter after be made into mixed-culture medium: add the material of following content at cynoglossus semilaevis cultivation waste water,

SODIUMNITRATE content is 0-15mg/L;

The biphosphate sodium content is 0-1mg/L;

Ironic citrate content is 0-1mg/L;

Sodium bicarbonate content is 0-0.5g/L;

Vitamins B₁Content is 0-100ug/L;

Vitamins B₁₂Content is 0-100ng/L;

(2) cultivate Zhanjiang Isochrysis galbana: with the mixed-culture medium that is made into, under 0-30 ℃ of temperature, pH4-8, illumination 500LX-10000LX, the initial inoculation density of Zhanjiang Isochrysis galbana is that 0.002-2mg/mL cultivates and can obtain a large amount of Zhanjiang Isochrysis galbana in 5-30 days.