CN101809155A - Transgenic plants with increased stress tolerance and yield - Google Patents

Abstract

Polynucleotides are disclosed which are capable of enhancing a growth, yield under water-limited conditions, and/or increased tolerance to an environmental stress of a plant transformed to contain such polynucleotides. Also provided are methods of using such polynucleotides and transgenic plants and agricultural products, including seeds, containing such polynucleotides as transgenes.

Description

Have the stress tolerance of increase and the transgenic plant of output

The application's requirement is filed in the benefit of priority of the U.S. Provisional Patent Application sequence number 60/953562 on August 2nd, 2007, and the content of this application is incorporated this paper by reference into.

Invention field

Generally speaking, the present invention relates to the encode transgenic plant of nucleotide sequence of following polypeptide of overexpression, described polypeptide can be given the stress tolerance of increase and increase thus under normal or abiotic stress condition plant-growth and crop yield.In addition, the present invention relates to the nucleotide sequence of the novel following polypeptide of separated coding, described polypeptide is given to plant: under the abiotic stress condition; the tolerance that increases; and/or, under the normal or abiotic stress condition, the plant-growth of increase and/or the output of increase.

Background of invention

Abiotic environment is coerced, and for example arid, salinity, hot and cold are the major limitation sexual factors of plant-growth and crop yield.Crop yield is defined as the bushel quantity of the relevant agricultural prods (for example grain (grain), forage (forage) or seed) of every acre of results in this article.These crop loss and crop yield losses of coercing the various crop (for example soybean, rice, Semen Maydis, cotton and wheat) that causes are remarkable economical and political factor, and they cause the food shortage in a lot of non-developed countries.

The water utilizability is abiotic coerces and to the importance of the influence of plant-growth.Continue to be exposed to the great change that drought condition causes plant metabolism, this finally causes necrocytosis and causes production loss thus.Because the water that the high content of salt in some soil causes cell to take in is less, high salt concentration produces plant and is similar to the influence of arid to plant.In addition, under the refrigerated temperature, owing to form ice in the plant, vegetable cell is lost water.Therefore, arid, heat, salinity and cold coerce the crop damage that causes main all since dehydration cause.

Because plant is the general environment that the feedwater utilizability reduces, the exsiccant protective mechanism that most of plants have all been evolved out and caused at abiotic stress of exposing during its life cycle.But,, can have a significant impact growth, growth, plant size and the output of most of crop plants if the seriousness of drying conditions is too high and the time length is oversize.Therefore, developing the plant that effectively makes water is possible can worldwide significantly improve human lives's strategy.

Traditional plant breeding strategy is relatively slow, and needs the basic strain and the hybridization of other germplasm of abiotic stress tolerance, to develop new abiotic stress resistance strain.The uncompatibility of hybridizing between the plant species that limited germplasm origin and family far away are relevant for this type of basic strain has been represented the prominent question that runs in the traditional breeding method.Also success far away of breeding at tolerance.

A lot of Agricultural biotechnologies companies have attempted to identify the gene that can give the tolerance that abiotic stress is replied, make great efforts the genetically modified abiotic stress tolerance crop plants of exploitation.Though it is analyzed to relate to some genes of stress response in the plant or water use efficiency,, still quite incomplete to the analysis and the clone of the plant gene of giving stress tolerance and/or water use efficiency, also only be the fragment formula.Up to now, the success that obtains on exploitation transgenosis abiotic stress tolerance crop plants is limited, does not also have this type of plant to become commercialized.

In order to develop the crop plants of transgenosis abiotic stress tolerance, must in research of the greenhouse of model plant system, crop plants and field test, check a plurality of parameters.For example, water use efficiency (WUE) is usually the parameter relevant with drought tolerance.Plant also is used to measure tolerance or the resistance of plant to abiotic stress to the research of replying of dry, osmotic shock and extreme temperature.When at genetically modified existence the influence of the stress tolerance of plant being tested, it is greenhouse or the plant-growth room environmental Inherent advantage than the field that soil property, temperature, water and plant recovery of nutrient and light intensity are carried out standardized ability.

WUE is defined and measures by number of ways.A kind of means are to calculate the ratio of the weight of the water that consumes during whole strain plant dry weight and the plant life.Another variation is when measuring biomass accumulation and water utilization, adopts the short timed interval.Also means are used the limitation measurement partly from plant, for example, only measure the growth and the water utilization of over-ground part.WUE also is defined as the partial C O from leaf or leaf₂The ratio of picked-up and water vapour loss, this measures (for example several seconds/several minutes) usually on very short time durations.The ratio of fixed 13C/12C also is used to assessment and utilizes C in the plant tissue of measuring with isotope ratio mass spectrometer₃WUE in the photosynthetic plant.

The efficient of the relative raising with water consumption of increase indication growth of WUE changes but this information can not show a kind of change or both in these two kinds of processes when adopting separately.When selecting to be used to improve the proterties of crop,, the WUE increase (growth does not change) that the water utilization minimizing causes has special benefit because will importing in the higher Irrigation farming system of expense at water.Increase the WUE increase (and not having corresponding water utilization to increase in a leap) that drives mainly due to growth and will can be used for all agrosystems.In the not limited a lot of agrosystems of water supply, the increase of growth is even it also can increase output along with the increase cost (that is, WUE does not have change) of water utilization.Therefore, need the novel method of increase WUE and biomass accumulation to improve agricultural productive force.

Follow measurement, also measure the parameter of indication transgenosis the potential impact of crop yield to the parameter relevant with abiotic stress tolerance.For leaf class crop, for example, clover, silage corn and hay, phytomass is relevant with ultimate production.But for cereal grain crops, come estimated output with other parameter, for example plant size (measuring by total plant dry weight), over-ground part dry weight, over-ground part fresh weight, leaf area, caulome are long-pending, plant height, lotus throne diameter, leaf length, root length, root amount, tillering quantity and number of sheets amount.Usually, the plant size of early development stage will be relevant with the plant size of growing late period.Usually, have plant can be smaller greatly the more light of plant absorbing and the carbonic acid gas of big leaf area, therefore during identical, may obtain more weight.Except possible microenvironment continue or hereditary advantage, plant must just obtain bigger size at first.There are the intensive inherited genetic factors in plant size and growth velocity, and therefore for a series of several genes types, the plant size under a kind of envrionment conditions may be relevant with the size under the another kind of condition.By this way, be similar to the various and dynamic environment that field crops ran in different positions and time with standard environment.

The ratio of harvest index---seed production and over-ground part dry weight, all relatively stable under a lot of envrionment conditionss, so may strong correlation between plant size and the grain output.Plant size and grain output inner link, plant stem-leaf flows or the photosynthesis productivity of storage because the major part in the grain biomass all depends on.Therefore, be exposed to the plant that may show the output of increase when the field is tested by selecting plant size (or even growing commitment) to filter out.As for abiotic stress tolerance, in growth room or the greenhouse under the standard conditions measurement of the plant size during to early development be the standard practices of measuring the possible output advantage that genetically modified existence gives.

Therefore, people need identify at stress tolerant plants and/or make other gene of expressing in the effective plant in the water, and they can give the water use efficiency of stress tolerance and/or increase to host plant and other plant species.New stress tolerant plants that produces and/or the plant with water use efficiency of increase will have lot of advantages, and for example, the scope that can plant crop plants increases, and for example realizes by the water demand that reduces plant species.What other advantage of wanting comprised increase responds to the lodging of wind, rain, insect or disease, crooked resistance to branch or stem.

Summary of the invention

The present invention is open: with some polynucleotide conversion plant, when described polynucleotide are present in the plant as transgenosis, cause plant-growth and to the enhancing of replying of environment-stress, thereby increased the output of the agricultural prods of plant.Can mediate these type of enhanced polynucleotide separates from colea (Brassica napus), rice (Oryza sativa), soybean (Glycine max), wheat (Triticum aestivum), barley (Hordeum vulgare), corn (Zea mays) and flax (Linum usitatissimum), and be listed in the table 1, its sequence is shown in the sequence table shown in the table 1.

Table 1

Gene I	Organism	Polynucleotide SEQ ID NO	Amino acid SEQ ID NO
				??BN51364980	Colea	??1	??2
??OS34096188	Rice (O.sativa)	??3	??4
				??OS32583643	Rice	??5	??6
??GM53626178	Soybean (G.max)	??7	??8
				??TA56540264	Wheat (T.aestiyum)	??9	??10
??BN45206322	Colea	??11	??12
				??GM48923793	Soybean	??13	??14
??TA55969932	Wheat	??15	??16
				??BN47310186	Colea	??17	??18
Gene I	Organism	Polynucleotide SEQ ID NO	Amino acid SEQ ID NO
				??BN51359456	Colea	??19	??20
??HV62552639	Barley (H.vulgare)	??21	??22
				??ZM61995511	Corn (Z.mays)	??23	??24
??LU61567101	Flax (L.usitatissimum)	??25	??26
				??LU61893412	Flax	??27	??28
??OS39781852	Rice	??29	??30

Gene I	Organism	Polynucleotide SEQ ID NO	Amino acid SEQ ID NO
				??OS34701560	Rice	??31	??32
??OS36821256	Rice	??33	??34
				??GM51659494	Soybean	??35	??36
??GM49780101	Soybean	??37	??38
				??GM59637305	Soybean	??39	??40
??TA55974113	Wheat	??41	??42

In one embodiment, the invention provides through comprising the expression cassette transgenic plant transformed of separated following polynucleotide, described polynucleotide encoding methionine sulfoxide reductase family protein, it is selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 and SEQ ID NO:10, the SelR protein zone of perhaps encoding.

In another embodiment, the invention provides through comprising the expression cassette transgenic plant transformed of separated following polynucleotide, described polynucleotide encoding homeodomain-leucine zipper protein matter, it has sequence shown in SEQ ID NO:12, SEQ ID NO:14 or the SEQ ID NO:16.

In another embodiment, the invention provides through comprising the expression cassette transgenic plant transformed of separated following polynucleotide, described polynucleotide encoding contains the zinc finger protein matter in A20 zone and AN1 zone, be selected from SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42 and SHQ ID NO:44, the zinc finger protein matter structural domain of perhaps encode AN1-sample and A20-sample.

In another embodiment, the present invention relates to the seed that the transfer-gen plant among the present invention produces, wherein seed is comprise above-mentioned polynucleotide genetically modified purebred.Plant from seed of the present invention is compared with the various plant of wild-type, under normal or the condition of coercing, the tolerance of environment-stress pressure is increased, and/or plant strain growth increases, and/or output increases.

On the other hand, the present invention relates to by the part of transfer-gen plant of the present invention, its plant or its seed production or by its product of making, such as food, feed, food tonic, feed tonic, makeup or medicine.

The polypeptide of being identified in isolating polynucleotide of being identified in the table 1 below the present invention also provides and the table 1.The present invention also specifically implements with the recombinant vectors that comprises isolating polynucleotide of the present invention.

In another embodiment, the present invention relates to the method for making above-mentioned transfer-gen plant, wherein this method comprises with the expression vector transformed plant cell that contains the isolating polynucleotide of the present invention, produces the transfer-gen plant of expressing this polynucleotide encoded polypeptides from this plant cell.Compare with the multiple plant of wild-type, the expression of this polypeptide in plant makes plant normal and/or have under the condition of coercing, and increased tolerance and/or plant strain growth and/or output to environment-stress pressure.

In another embodiment, the invention provides the increase plant to the tolerance of environment-stress and/or the method for growth and/or output.Described method comprises the steps: with the expression cassette transformed plant cells that comprises separated polynucleotide of the present invention, and from vegetable cell generation transgenic plant, wherein said transgenic plant comprise described polynucleotide.

Summary of drawings

The comparison of Fig. 1: BN51364980, OS34096188, OS32583643, GM53626178, TA56540264 and known methionine sulfoxide reductase family protein.

The comparison of Fig. 2: BN45206322, GM48923793 and TA55969932 and known homeodomain-leucine zipper protein matter.

Fig. 3: BN47310186, BN51359456, HV62552639, ZM61995511, LU61567101, LU61893412, OS39781852, OS34701560, OS36821256, GM51659494, GM49780101, GM59637305 and TA55974113 and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain.

Preferred implementation describes in detail

In this application, many pieces of open source literatures have been mentioned.Those reference of mentioning in all these open source literatures and these open source literatures all by reference integral body incorporate the application into, more completely to explain the state in field of the present invention.Term used herein only is in order to describe the purpose of specific implementations, but not is intended to be limited.When using in this article, that " one/kind " (" a " or " an ") can represent is one or more/kind, this depends on its employed context.Therefore, for example, mentioning " cell " can represent to use at least one cell.

In one embodiment, the invention provides the separated polynucleotide of evaluation in the overexpression table 1 or the transgenic plant of its homologue.Transgenic plant of the present invention show than the wild-type kind of the plant tolerance to the increase of environment-stress.Randomly, than the wild-type kind of plant, under normal or stress conditions, the overexpression of this type of separated nucleic acid in plant can cause the increase of plant-growth or the output of relevant agricultural prods to increase.Wish to be bound by any theory, transfer-gen plant of the present invention is to the increase of the tolerance of environment-stress, and the increase of growth and/or the increase of output are believed what the increase by plant water service efficiency (water use efficiency) caused.

As definition herein, " transgenic plant " are such plants, have used recombinant DNA technology that it is transformed, make it contain separated, the original nucleic acid that is not present in the plant.When using in this article, term " plant " comprises whole strain plant, vegetable cell and plant part.Plant part includes but not limited to, stem, root, ovule, stamen, leaf, embryo, meristematic tissue zone, callus, gametophyte, sporophyte, pollen, sporule etc.Transgenic plant of the present invention can be male sterile or male fertile, and can comprise not being to comprise the genetically modified transgenosis through the separation polynucleotide as herein described.

When using in this article, term " kind " refers to share in the species plant group of constant characteristic, and described feature is separated they and canonical form and other possibility kind in these species.Though have at least a distinctive proterties, kind also makes a variation by between intravarietal each individuality some and characterizes, and this mainly separates based on Mendelian character between the offspring of later generation.If for certain specific trait, isozygoty in the heredity---to such degree: the kind of pure breeding is by self-pollination, do not observe freely the separating of proterties of significant quantity in the offspring, and kind just is considered to " pure breeding " of specific trait.In the present invention, proterties is that transgene expression from one or more separated polynucleotide of introduced plant kind produces.As also employed in this article, term " wild-type kind " refers to that plant is at comparing purpose to its one group of plant being analyzed so in contrast, wherein, wild-type product kind of plant is identical with transgenic plant (through separated polynucleotide plant transformed according to the present invention), but wild-type product kind of plant does not transform through separated polynucleotide of the present invention.

As definition herein, term " nucleic acid " and " polynucleotide " but mutual alternative, their expression straight chains or branched, strand or double-stranded RNA or DNA or its crossbred.This term also comprises the RNA/DNA crossbred." separated " nucleic acid molecule is such: its basically with described nucleic acid natural origin in other nucleic acid molecule sequence of other polypeptide (that is, encode) of existing separately.For example, the nucleic acid through the clone is considered to separated.If nucleic acid is changed by artificial interference, perhaps being placed in not is on the locus or position in its natural site, if perhaps it is introduced into cell by conversion, it is separated that nucleic acid just is considered to.In addition, separated nucleic acid molecule, for example the cDNA molecule can not contain its natural some relevant other cell materials when producing by recombinant technology, when by chemosynthesis, does not contain precursor or other chemical.Though be positioned at genes encoding zone 3 ' and 5 ' terminal non-translated sequence its optional comprising, it has preferably removed the sequence of the natural flank of coding region in its naturally occurring replicon.

When using in this article, term " environment-stress " refers to the suboptimum condition relevant with salinity, arid, nitrogen, temperature, metal, chemical, pathogenicity bo or oxidative stress or its any combination.Term " water use efficiency " and " WUE " refer to the amount of the amount of the organic matter that plant produces divided by the organic employed water of plant production, that is, the dry weight of plant is with respect to the water utilization of plant.When using in this article, term " dry weight " refers to all substances outside the water in the plant, and it comprises, for example, and carbohydrate, albumen, oil and mineral matter nutritional thing.

Any plant species can be transformed, to produce according to transgenic plant of the present invention.Transgenic plant of the present invention can be dicotyledons or monocotyledons.For example, and and without limitation, transgenic plant of the present invention can come from any in the following dicotyledons section: pulse family (Leguminosae) comprises plants such as pea, clover and soybean; Umbelliferae (Umbelliferae) comprises plants such as Radix Dauci Sativae and celery; Solanaceae (Solanaceae) comprises plants such as tomato, potato, eggplant, tobacco and pepper; Cruciferae (Cruciferae), particularly Btassica (Brassica), it comprises plants such as rape (oilseed rape), beet, wild cabbage, Cauliflower and cabbage; And Arabidopis thaliana (A.thaliana); Composite family (Compositae), it comprises plants such as lettuce; Malvaceae (Malvaceae), it comprises cotton; Pulse family (Fabaceae), it comprises plants such as peanut, or the like.Transgenic plant of the present invention can come from monocotyledons, for example, and wheat, barley, Chinese sorghum, grain, rye, triticale, corn, rice, oat and sugarcane.Transgenic plant of the present invention also comprise tree, and for example apple, Li, Wen outer coffin, Lee, cherry, peach, nectarine, apricot, papaya, mango and other woody species comprise coniferals and the tree of the class of falling leaves, for example willow, pine, Chinese larch, cdear, Oak Tree etc.Especially preferred is Arabidopis thaliana, tobacco (Nicotiana tabacum), rape, soybean, Semen Maydis, wheat, flax, potato and Flower of Aztec Marigold.

As shown in table 1, one embodiment of the present invention are through comprising the expression cassette transgenic plant transformed of separated following polynucleotide, described polynucleotide encoding methionine sulphoxide reductase enzyme family protein.The methionine sulphoxide (MetSO) that methionine sulphoxide reductase enzyme (MSR) catalysis Trx matter relies on is reduced to correct methionine residue.Methionine(Met) is easy to be subjected to oxidative damage, and the methionine(Met) oxidation causes the modification on numerous protein activity and the conformation.

Two kinds of MSR are arranged, A type and Type B; But these two types all uncorrelated on sequence and structure.MSRB enzyme selectivity ground catalytic reduction methionine sulphoxide R enantiomorph.MSRB type protein contains four conserved cysteine residue in two CxxC motifs, wherein x can be any amino acid.These CxxC motifs might be fixing relevant with zinc.

Described the example from several plant species, wherein the condition of environment-stress has caused the level of the active chalcogen (ROS) of increase, and consequent oxidative damage has caused the modification of MSR genetic expression.MSR itself is the good candidate of direct antioxidant, because oxidation of methionine residue round-robin and reduction can be served as the effective way of eliminating active chalcogen in the cell.In eukaryote, old and feeble and multiple disease all is that the protein structure that caused by the methionine(Met) oxidation and the destruction of function are caused.The proteinic substrate of MSR much still is the number of the unknown.Up to now, first certified plant MSR substrate is little plastid (plastidic) heat shock protein Hsp21.Hsp21 comprises conservative N end regions, and it highly is rich in methionine residue.This methionine(Met) zone must keep the reduction form, to keep the chaperone-like activity of Hsp21.

Transfer-gen plant in this embodiment may comprise the polynucleotide of any coding methionine sulphoxide reductase enzyme family protein.Transfer-gen plant in this embodiment preferably includes the polynucleotide of coding SelR structural domain, and the sequence that this structural domain contains comprises the amino acid 77 to 199 among the SEQ ID NO:2; Amino acid 79 to 200 among the SEQ ID NO:4; Amino acid 91 to 213 among the SEQ ID NO:6; Amino acid 79 to 200 among the SEQ ID NO:8; Amino acid 80 to 201 among the SEQ ID NO:10.Transfer-gen plant in this embodiment more preferably comprises the polynucleotide of coding methionine sulphoxide reductase enzyme family protein, and the sequence of described methionine sulphoxide reductase enzyme family protein comprises the amino acid/11 to 205 among the SEQ ID NO:2; Amino acid/11 to 204 among the SEQ ID NO:4; Amino acid/11 to 214 among the SEQ ID NO:6; Amino acid/11 to 202 among the SEQ ID NO:8; Amino acid/11 to 206 among the SEQ ID NO:10.

In another embodiment, the invention provides the transfer-gen plant of the expression cassette conversion of using the isolating polynucleotide that contains coding homeodomain-leucine zipper protein matter.Homeodomain-leucine zipper (HDZip) protein belongs to transcription factor family, and it interacts by leucine zipper motif as dimer, and by its homeodomain in sequence-specific mode in conjunction with DNA.Based on sequence, the HDZip family protein is divided into four classes.It is believed that I class HDZip protein regulates the reaction of plant to ABA, and may have the relevant control effect of signal conduction with ABA.The proteinic member of I class HDZip can be at external formation heterodimer, and therefore, this class can constitute the protein interaction network, and its mediation is gathered to regulate similar target gene the reaction and/or the integrated signal of environmental stimulus.

Transfer-gen plant in this embodiment may comprise the polynucleotide of any coding homeodomain-leucine zipper protein matter.Transfer-gen plant in this embodiment preferably includes the polynucleotide of coding homeodomain, and the sequence of described homeodomain comprises the amino acid 62 to 116 among the SEQ ID NO:12; Amino acid 83 to 147 among the SEQ ID NO:14;Amino acid 76 to 130 among the SEQ ID NO:16; The perhaps polymerized nucleoside acid encoding homeodomain relevant with leucine zipper motif, its sequence comprises the amino acid/11 17 to 161 among the SEQID NO:12; Amino acid/11 38 to 182 among the SEQ ID NO:14; Amino acid/11 31 to 175 among the SEQ ID NO:16.Transfer-gen plant in this embodiment more preferably comprises the polynucleotide of coding homeodomain-leucine zipper protein, and the sequence of described homeodomain-leucine zipper protein comprises the amino acid/11 to 310 among the SEQ ID NO:12; Amino acid/11 to 331 among the SEQ ID NO:14; Amino acid/11 to 340 among the SEQ ID NO:16.

In another embodiment, the invention provides the transfer-gen plant that expression cassette transforms, the contained isolating polymerized nucleoside acid encoding of this expression cassette contains the zinc finger protein matter of A20 and AN1 structural domain.The zinc finger protein matter that contains A20 and AN1 structural domain is present in all eukaryotes.These proteinic characteristics are to exist to contain A20 Zinc finger domain and the AN1 Zinc finger domain that a plurality ofhalfcystine 2/halfcystines 2 refer to motif.The AN1 structural domain is normally in the protein that comprises the A20 Zinc finger domain.The function of these protein in animal system characterized well, but the function of these protein in plant known little about it.

Rice OsiSAP1 protein is the plant protein of first certified A20 of having and AN1 Zinc finger domain.It is relevant with multiple coercive that this protein is found.This OsiSAP1 gene is induced when response environment is coerced such as cold, salinity, arid, water logging, injured and heavy metal.The also known lineal homology that comes from beans is handled and is tackled by inducer (eliciter) and can be induced when injured.When crossing expression in tobacco, OsiSAP1 produces abiotic stress tolerance.Therefore OsiSAP1 does not have typical nuclear localization signal, is considered to the function by Zinc finger domain being exercised protein interactions.

Transfer-gen plant in this embodiment comprises the polynucleotide that any coding includes the zinc finger protein matter of A20 territory and AN1 structural domain.Transfer-gen plant in this embodiment preferably includes polynucleotide, and the A20 sample zinc of its coding refers to that the sequence that is had comprises the amino acid/11 5 to 39 among the SEQ ID NO:18; Amino acid/11 3 to 37 among the SEQ ID NO:20; Amino acid/11 5 to 39 among the SEQ ID NO:22; Amino acid/11 4 to 38 among the SEQ ID NO:24; Amino acid/11 4 to 38 among the SEQ ID NO:26; Amino acid 40 to 64 among the SEQ ID NO:28; Amino acid/11 5 to 39 among the SEQ ID NO:30; Amino acid/11 9 to 43 among the SEQ ID NO:32; Amino acid/11 3 to 37 among the SEQ ID NO:34; Amino acid/11 9 to 43 among the SEQ ID NO:36; Amino acid/11 8 to 42 among the SEQ ID NO:38; Amino acid/11 5 to 39 among the SEQ ID NO:40; Amino acid/11 5 to 39 among the SEQ ID NO:42; Amino acid/11 9 to 43 among the SEQ ID NO:44, and this polymerized nucleoside acid encoding AN1 sample Zinc finger domain, its sequence comprises the amino acid/11 18 to 158 among the SEQ ID NO:18; Amino acid/11 28 to 168 among the SEQ IDNO:20; Amino acid 95 to 135 among the SEQ ID NO:22; Amino acid/11 12 to 152 among the SEQID NO:24; Amino acid/11 15 to 155 among the SEQ ID NO:26; Amino acid/11 79 to 219 among the SEQ ID NO:28; Amino acid/11 10 to 150 among the SEQ ID NO:30; Amino acid/11 05 to 145 among the SEQ ID NO:32; Amino acid/11 05 to 145 among the SEQ ID NO:34; Amino acid/11 11 to 151 among the SEQ ID NO:36; Amino acid/11 02 to 142 among the SEQ ID NO:38; Amino acid/11 13 to 153 among the SEQ ID NO:40; Amino acid/11 15 to 155 among the SEQ ID NO:42; Amino acid/11 06 to 146 among the SEQ ID NO:44.Transfer-gen plant in this embodiment more preferably comprises polynucleotide, and the sequence of the zinc finger protein matter that contains A20 and AN1 structural domain of its coding comprises the amino acid/11 to 177 among the SEQ ID NO:18; Amino acid/11 to 187 among the SEQ IDNO:20; Amino acid/11 to 154 among the SEQ ID NO:22; Amino acid/11 to 171 among the SEQ ID NO:24; Amino acid/11 to 174 among the SEQ ID NO:26; Amino acid/11 to 239 among the SEQ ID NO:28; Amino acid/11 to 169 among the SEQ ID NO:30; Amino acid/11 to 164 among the SEQ ID NO:32; Amino acid/11 to 164 among the SEQ ID NO:34; Amino acid/11 to 170 among the SEQ ID NO:36; Amino acid/11 to 161 among the SEQ ID NO:38; Amino acid/11 to 172 among the SEQ ID NO:40; Amino acid/11 to 174 among the SEQ ID NO:42; Amino acid/11 to 165 among the SEQ ID NO:44.

The present invention also provides the seed by the transgenic plant generation of the listed polynucleotide of expression table 1; wherein; described seed contains described polynucleotide; and wherein; described plant is than the wild-type kind of plant, growth that increases under normal or stress conditions and/or output and/or be pure breeding aspect the tolerance of the increase of environment-stress.The present invention also provide by or the product produced from the transgenic plant of expressing described polynucleotide, its plant part or its seed.Can use several different methods well known in the art to obtain product.When using in this article, word " product " includes but not limited to food, feed, food fill-in, feed supplement, makeup or medicine.Food is considered to the composition that is used for nutrition or is used to supplement the nutrients.Animal-feed and animal-feed fill-in are considered to food especially.The present invention also provides the agricultural prods by any production in transgenic plant, plant part and the plant seed.Agricultural prods includes but not limited to: plant milk extract, albumen, amino acid, carbohydrate, fat, oil, polymkeric substance, VITAMIN etc.

A kind of preferred embodiment in, separated polynucleotide of the present invention comprise following polynucleotide, it has the sequence that is selected from the polynucleotide sequence that table 1 lists.These polynucleotide can comprise sequence and the 5 ' non-translated sequence and the 3 ' non-translated sequence of coding region.

Polynucleotide of the present invention can use the Protocols in Molecular Biology of standard to separate with the sequence information that provides here.The synthetic oligonucleotide primer that is used for PCR amplification can design based on the nucleotide sequence shown in the table 1.Nucleic acid molecule of the present invention can be with cDNA or alternatively, is template and suitable oligonucleotide primer with genomic dna, by the amplification of Standard PC R amplification technique.Kuo Zeng nucleic acid molecule can be cloned in the appropriate carriers like this, characterizes with dna sequence analysis.In addition, can for example, use automatic dna synthesizer with the synthetic technology preparation of standard with the corresponding oligonucleotide of nucleotide sequence of listing in the table 1.

" homologue " is defined as having respectively two nucleic acid or the polypeptide of similar or essentially identical Nucleotide or aminoacid sequence in this article.Homologue comprises allelic variant, analogue and directly to homologue, they such as hereinafter definition.When using in this article, term " analogue " refers to have same or similar function but two nucleic acid of evolving respectively in incoherent biology.When using in this article, but term " directly to homologue " refers to from different plant species by species formation from two next nucleic acid of common ancestral gene evolution.The term homologue also comprises because genetic codon degeneracy and nucleic acid molecule different with one of nucleotide sequence shown in the table 1 but the same polypeptide of encoding.When using in this article, " naturally occurring " nucleic acid molecule refers to have the RNA or the dna molecular (natural polypeptides of promptly encoding) of the nucleotide sequence that occurring in nature exists.

For (for example measuring two aminoacid sequences, one of peptide sequence of table 1 and homologue thereof) per-cent sequence identity, with regard to optimum comparison purpose, (for example with sequence alignment, can in the sequence of a polypeptide, introduce breach, to align) with other polypeptide or nucleic acid optimum.Amino-acid residue on the more corresponding then amino acid position.When the position in the sequence by another sequence in same amino-acid residue on the corresponding position when occupying, molecule is exactly identical in this position.Can between two nucleotide sequences, carry out the comparison of same-type.

Preferably, the separated amino acid homology thing of polypeptide of the present invention, analogue and straight to the about at least 50-60% of the whole piece aminoacid sequence shown in homologue and the table 1, preferably about at least 60-70%, more preferably about at least 70-75%, 75-80%, 80-85%, 85-90% or 90-95%, most preferably about at least 96%, 97%, 98%, 99% is identical or more identical.Another preferred embodiment in, separated nucleic acid homologue of the present invention comprises following nucleotide sequence, nucleotide sequence shown in described nucleotide sequence and the table 1 is 40-60% at least approximately, preferably about at least 60-70%, more preferably about at least 70-75%, 75-80%, 80-85%, 85-90% or 90-95%, further preferably about at least 95%, 96%, 97%, 98%, 99% is identical or more identical.

(Carlsbad CA92008) measures per-cent sequence identity between two nucleic acid or the peptide sequence for Invitrogen, 1600 FaradayAve. can for example to use Vector NTI 9.0 (PC) software package.When using Vector NTI, for measuring the per-cent identity of two nucleic acid, the open point penalty of spendable breach is 15, and it is 6.66 that breach extends point penalty.For measuring the per-cent identity of two polypeptide, the open point penalty of spendable breach is 10, and it is 0.1 that breach extends point penalty.All other VectorNTI parameters all can be provided with according to default setting.With regard to the right purpose of the multiple ratio of using Vector NTI (Clustal W algorithm), the open point penalty of breach is 10, and it is 0.05 that breach extends point penalty, uses blosum 62 matrixes.Alternatively, (Myers and Miller (1989) CABIOS 4,11-17), all parameters are set to default setting can to use Align 2.0.Should be appreciated that when comparison dna sequence and RNA sequence, thymidylic acid is equal to uridylate with regard to the purpose of measuring sequence identity.

Homologue, analogue and straight nucleic acid molecule to homologue corresponding to the listed polypeptide of table 1 can separate with the identity of described polypeptide based on it, wherein use the polynucleotide of each polypeptide of coding or based on its primer as hybridization probe, carry out under tight hybridization conditions according to the standard hybridization technique.About the blot hybridization of DNA to DNA, term " stringent condition " refers to hybridize in 60 ℃ in the salmon sperm dna of 10xDenhart ' s solution, 6X SSC, 0.5%SDS and 100 μ g/ml sex change herein.At 62 ℃, 3X SSC/0.1% SDS follows among 1X SSC/0.1%SDS and the last 0.1X SSC/0.1%SDS trace is washed 30 minutes (at every turn) in regular turn.In addition, when using in this article, a kind of preferred embodiment in, term " stringent condition " refers to hybridize in 65 ℃ in 6X SSC solution.In another embodiment, " height stringent condition " refers to spend the night in 65 ℃ of hybridization in the salmon sperm DNA of 10XDenhart ' s solution, 6X SSC, 0.5%SDS and 100 μ g/ml sex change.At 65 ℃, 3X SSC/0.1%SDS follows among 1X SSC/0.1%SDS and the last 0.1X SSC/0.1%SDS trace is washed 30 minutes (at every turn) in regular turn.The method of nucleic acid hybridization is at document Meinkoth and Wahl, and 1984, description is arranged among the Anal.Biochem.138:267-284; Be that well known in the art (see that such as, Current Protocols in MolecularBiology,Chapter 2, Ausubel etc. compile Greene Publishing and Wiley-Interscience, New York, 1995; And Tijssen, 1993, LaboratoryTechniques in Biochemistry and Molecular Biology:Hybridizationwith Nucleic Acid Probes, part i, the 2nd chapter, Elsevier, New York, 1993).Preferably, the of the present invention separated nucleic acid molecule of the nucleotide sequence hybridization of listing with table 1 under tight or height stringent condition is corresponding to naturally occurring nucleic acid molecule.

There is several different methods to can be used for producing the library of possible homologue from degenerate oligonucleotide sequence.Can in automatic dna synthesizer, carry out the chemosynthesis of degeneracy gene order, then the synthetic gene be connected into suitable expression vector.Use the degeneracy genome to allow in a kind of mixture, to provide all sequences of the group of the possible sequence that coding wants.The method that is used for synthetic degenerate oligonucleotide be known in the art (see, for example, Narang, 1983, Tetrahedron 39:3; Itakura etc., 1984, Annu.Rev.Biochem.53:323; Itakura etc., 1984, Science 198:1056; Ike etc., 1983, Nucleic Acid Res.11:477).

In addition, can make nucleic acid through optimizing.Preferably, the following polypeptide of nucleic acid encoding through optimizing, described polypeptide have the function similar to the listed those polypeptides of table 1 and/or when its can be under normal and/or water confined condition during overexpression in plant coordinate plant growth and/or output and/or to the tolerance of environment-stress, and more preferably, under normal and/or water confined condition, increase plant-growth and/or output and/or to the tolerance of environment-stress.When using in this article, " through what optimize " refers to through genetic modification to increase it at the given plant or the nucleic acid of the expression in the animal.For the nucleic acid through being optimized at plant is provided, the dna sequence dna of gene can be modified to: the codon that 1) comprises the plant gene preference of highly expressing; 2) comprise the A+T content in the basic nucleotide base composition of finding in the plant; 3) form the plant homing sequence; 4) cause RNA instability, inappropriate polyadenylation, degraded and terminated sequence with elimination, perhaps eliminate the sequence that forms secondary structure hair fastener or RNA splice site; Or 5) eliminate the antisense open reading-frame (ORF).Can select distribution frequency by utilizing the codon in general plant or the specified plant, realize the increase expression of nucleic acid in plant.Being used to optimize the method that the plant amplifying nucleic acid expresses can be in EPA 0359472, EPA 0385962, PCT application No.WO 91/16432, U.S. Patent No. 5,380,831, U.S. Patent No. 5,436,391, people such as Perlack, 1991, people such as Proc.Natl.Acad.Sci.USA 88:3324-3328 and Murray, 1989, find among the NucleicAcids Res.17:477-498.

Can be optimized separated polynucleotide of the present invention, make its codon select distribution frequency and the plant gene deviation of highly expressing preferably to be no more than 25%, more preferably no more than about 10%.In addition, consider the per-cent G+C content (monocotyledons seems that at this position preference G+C, dicotyledons is not then) of degeneracy the 3rd base.Will recognize that also XCG (wherein X is A, T, C or G) Nucleotide is least preferred codon in dicotyledons, and the XTA codon should be avoided all in unifacial leaf and dicotyledons.Nucleic acid through optimizing of the present invention preferably also has with selected host plant very avoids index near CG and TA doublet.More preferably, these indexes and host's deviation is no more than about 10-15%.

The present invention also provides separated recombinant expression vector; it comprises polynucleotide mentioned above; wherein; the expression of described carrier in host cell causes: than the wild-type kind of host cell; plant increases growth and/or output under normal or water confined condition, and/or increases the tolerance to environment-stress.Recombinant expression vector of the present invention comprises to be suitable for the nucleic acid of the present invention that the form of express nucleic acid exists in host cell, this means, recombinant expression vector comprises one or more regulating and controlling sequence, it is based on waits to be used that the host cell that is used to express selects, and it links to each other effectively with nucleotide sequence to be expressed.When using about recombinant expression vector in this article, " link to each other effectively " and be intended to represent that interested nucleotide sequence (for example links to each other in the mode that allows nucleotide sequence to express with regulating and controlling sequence, when carrier is introduced in the host cell, in bacterium or plant host cell).Term " regulating and controlling sequence " is intended to comprise promotor, enhanser and other expression controlling elements (for example poly-adenosine signal).This type of regulating and controlling sequence is well known in the art.Regulating and controlling sequence comprise instruct nucleotides sequence be listed in constitutive expression in polytype host cell those and only under some host cell or some condition, instruct those that nucleotide sequence expresses.It will be appreciated by those skilled in the art that the design to expression vector can be depending on following factor, for example, treat the selection of transformed host cells, the polypeptide expression level of wanting etc.Expression vector of the present invention can be introduced host cell, to produce the polypeptide of nucleic acid encoding as herein described thus.

Gene expression in plants should link to each other effectively with suitable promotor, and described promotor is given in time, the genetic expression of cell-specific or tissue specificity mode.The promotor that can be used for expression cassette of the present invention comprises can start any promotor of transcribing in vegetable cell.This type of promotor includes but not limited to: those that can obtain from plant, plant virus and bacterium (containing the gene of expressing plant, for example Agrobacterium (Agrobacterium) and rhizobium (Rhizobium))

Promotor can be composing type, induction type, the etap is preferential, cell type is preferential, tissue is preferential or organ is preferential.Constitutive promoter all has activity under most of conditions.The example of constitutive promoter comprises CaMV 19S and 35S promoter (Odell etc., 1985, Nature313:810-812), sX CaMV 35S promoter (Kay etc., 1987, Science236:1299-1302), the Sep1 promotor, rice actin promoter (McElroy etc., 1990, Plant Cell 2:163-171), Arabidopis thaliana actin promoter, ubiquitin (ubiquitan) promotor (Christensen etc., 1989, Plant Molec.Biol.18:675-689), pEmu (Last etc., 1991, Theor.Appl.Genet.81:581-588), radix scrophulariae mosaic virus 35 S promoter, Smas promotor (Velten etc., 1984, EMBO J 3:2723-2730), super promotor (U.S. Patent No. 5,955,646), the GRP1-8 promotor, cinnamyl-alcohol dehydrogenase promotor (U.S. Patent No. 5,683,439), promotor from the T-DNA of Agrobacterium, for example, mannopine synthase, nopaline synthase and octopine synthase, diphosphoribulose carboxylase small subunit (ssu-RUBISCO) promotor etc.

Inducible promoter prefers under some envrionment conditions has activity, for example, exist or when not having nutrition or meta-bolites, heat or cold, illumination, pathogenic agent attack, anaerobic condition etc.For example, the hsp80 promotor from Btassica (Brassica) is a heat-inducible; The PPDK promotor is by photoinduction; Can pass through the infection induced of pathogenic agent from tobacco, Arabidopis thaliana and zeistic PR-1 promotor; The Adh1 promotor is by anoxic and cold stress-inducing.Also can assist gene expression in plants (summary referring to Gatz, 1997, Annu.Rev.Plant Physiol.Plant Mol.Biol.48:89-108) by inducible promoter.If want genetic expression to take place in the temporal mode, but the promotor of chemical induction is especially suitable.The example of this type of promotor is salicylic acid inducible promotor (PCT applies for No.WO 95/19443), tsiklomitsin inducible promoter (people such as Gatz, 1992, Plant J.2:397-404) and alcohol induced type promotor (PCT applies for No.WO 93/21334).

Of the present invention a kind of preferred embodiment in, inducible promoter is a stress induced promoter.With regard to purpose of the present invention, stress induced promoter prefers to one or more following coercing has activity down: the suboptimum condition relevant with salinity, arid, nitrogen, temperature, metal, chemical, pathogenic agent and oxidative stress.Stress induced promoter includes but not limited to Cor78 (people such as Chak, 2000, Planta 210:875-883; People such as Hovath, 1993, Plant Physiol.103:1047-1053), Cor15a (people such as Artus, 1996, PNAS 93 (23): 13404-09), Rci2A (people such as Medina, 2001, Plant Physiol.125:1655-66; People such as Nylander, 2001, Plant Mol.Biol.45:341-52; Navarre and Goffeau, 2000, EMBOJ.19:2515-24; People such as Capel, 1997, Plant Physiol.115:569-76), Rd22 (people such as Xiong, 2001, Plant Cell 13:2063-83; People such as Abe, 1997, Plant Cell9:1859-68; People such as Iwasaki, 1995, Mol.Gen.Genet.247:391-8), cDet6 (Lang and Palve, 1992, Plant Mol.Biol.20:951-62), ADH1 (people such as Hoeren, 1998, Genetics 149:479-90), KAT1 (people such as Nakamura, 1995, PlantPhysiol.109:371-4), KST1 (Muller-

Deng the people, 1995, EMBO14:2409-16), Rha1 (people such as Terryn, 1993, Plant Cell 5:1761-9; People such as Terryn, 1992, FEBS Lett.299 (3): 287-90), ARSK1 (people such as Atkinson, 1997, GenBank accession number L22302 and PCT application No.WO 97/20057), PtxA (people such as Plesch, GenBank accession number X67427), SbHRGP3 (people such as Ahn, 1996, Plant Cell8:1477-90), GH3 (people such as Liu, 1994, Plant Cell 6:645-57), pathogen-inducible PRP1 gene promoter (people such as Ward, 1993, Plant.Mol.Biol.22:361-366), thermal induction type hsp80-promotor (U.S. Patent No. 5187267) from tomato, cold induction type α-Dian Fenmei promotor (PCT applies for No.WO 96/12814) or wound-induced type pinII promotor (European patent No.375091) from potato.About other example of promotor of arid, cold-peace salt induction type, RD29A promotor for example, referring to people such as Yamaguchi-Shinozalei, 1993, Mol.Gen.Genet.236:331-340.

Preferential promotor preference of etap was expressed in some etap.Tissue and the preferential promotor of organ comprise those that preference is expressed in some tissue or organ (for example leaf, root, seed or xylem).Tissue example preferential and the organ preferential promoters includes but not limited to: fruit is preferential, ovule is preferential, male tissue is preferential, seed is preferential, integument is preferential, stem tuber is preferential, handle (stalk) preferentially, pericarp is preferential, leaf is preferential, column cap is preferential, pollen is preferential, flower pesticide is preferential, petal is preferential, sepal is preferential, bennet is preferential, silique is preferential, stem is preferential, the preferential promotor of root etc.The preferential promotor preference of seed is expressed in seed development and/or duration of germination.For example, the preferential promotor of seed can be that embryo is preferential, endosperm is preferential and plant skin preferential (see people such as Thompson, 1989, BioEssays10:108).The example of the preferential promotor of seed includes but not limited to cellulose synthase (celA), Ciml, γ-zein, sphaeroprotein-1, corn 19kD zein (cZ19B1) etc.

Preferential or the organ preferential promoters of other suitable tissue comprises the napin gene promoter (U.S. Patent No. 5 from rape (rapeseed), 608,152), USP promotor (people such as Baeumlein from broad bean (Vicia faba), 1991, Mol.Gen.Genet.225 (3): 459-67), oleosin promotor (PCT applies for No.WO98/45461) from Arabidopsis (Arabidopsis), phaseolin promoter (U.S. Patent No. 5 from Kidney bean (Phaseolus vulgaris), 504,200), Bce4 promotor (PCT applies for No.WO91/13980) or legumin B4 promotor (LeB4 from Btassica; People such as Baeumlein, 1992, PlantJournal, 2 (2): 233-9) and the promotor of in monocotyledons (for example corn, barley, wheat, rye, rice etc.), giving seed-specific expression.Noticeable suitable promotor is (from the hordein gene of barley from those promotors of describing among the Ipt2 of barley or Ipt1 gene promoter (PCR application No.WO 95/15389 and PCT application No.WO 95/23230) or the PCT application No.WO 99/16890, the paddy protein gene, rice paddy rice plain gene, the paddy alcohol soluble protein gene, the gliadine gene of wheat, the wheat gluten gene, the avenin gene, the promotor of the secalin gene of Chinese sorghum kasirin gene and rye).

Other promotor that can be used for expression cassette of the present invention includes but not limited to the conjugated protein promotor of main chloroplast(id) a/b, the histone promotor, the Ap3 promotor, β-conglycin promotor, oil card Nola rape protein promoter, the soybean agglutinin promotor, corn 15kD zein promotor, 22kD zein promotor, 27kD zein promotor, g-zein promotor, waxy, shrunken 1, shrunken 2 and bronze promotor and Zm13 promotor (U.S. Patent No. 5,086,169), corn polygalacturonase promotor (PG) (United States Patent(USP) Nos. 5,412,085 and 5,545,546) and SGB6 promotor (U.S. Patent No. 5,470,359) and synthetic or other natural promoter.

Other maneuvering ability that the control heterologous gene is expressed in plant can obtain by using DNA binding domains and response element (that is the DNA binding domains of originating from non-plant) from the allos source.The example of this type of allogeneic dna sequence DNA binding domains is LexA DNA binding domains (Brent and Ptashne, 1985, Cell 43:729-736).

Of the present invention a kind of preferred embodiment in, the polynucleotide that table 1 is listed are expressed in the vegetable cell from higher plant (for example spermatophyte, for example crop plants).Polynucleotide can comprise transfection, conversion or transduction, electroporation, partickle bombardment, agroinfection etc. by any means " introducing " vegetable cell.Be used to transform or the appropriate method of transfection of plant cells is disclosed, for example, use United States Patent(USP) Nos. 4,945,050,5,036,006,5,100,792,5,302,523,5,464,765,5,120,657,6,084, the partickle bombardment shown in 154 grades.More preferably, can use Agrobacterium-mediated Transformation to make transgenic corn seed of the present invention, as United States Patent(USP) Nos. 5,591,616,5,731,179,5,981,840,5,990,387,6,162,965,6,420,630, U.S. Patent Publication No. 2002/0104132 etc. are described.Can use for example European patent No.EP0424047, U.S. patent No.5,322,783, European patent No.EP 0,397 687, U.S. Patent No. 5,376,543 or U.S. Patent No. 5,169,770 described technology carry out the conversion to soybean.The special example that wheat transforms can be applied for finding among the No.WO 93/07256 in PCT.Can use United States Patent(USP) Nos. 5,004,863,5,159,135,5,846, disclosed method is come converting cotton in 797 grades.Can use United States Patent(USP) Nos. 4,666,844,5,350,688,6,153,813,6,333,449,6,288,312,6,365,807,6,329, disclosed method transforms rice in 571 grades.Other methods for plant transformation is disclosed in, for example, United States Patent(USP) Nos. 5,932,782,6,153,811,6,140,553,5,969,213,6,020, in 539 grades.Being suitable for that transgenosis is inserted any methods for plant transformation of specified plant all can be used according to the invention.

According to the present invention, if the polynucleotide of introducing are incorporated non-chromosome self-replicating into or are integrated in the plant chromosome, then it can be stablized and remains in the vegetable cell.Perhaps, but the polynucleotide of introducing can be present on the outer non-replicating vector of karyomit(e) and instantaneous conversion or have instantaneous activity.

Another aspect of the present invention relates to separated polypeptide, and it has the sequence that is selected from the peptide sequence that table 1 lists." separated " or " purified " polypeptide does not contain (when producing by recombinant DNA technology) some cell materials or (during chemosynthesis) chemical precursor or other chemical.This saying comprises such polypeptide prepared product " not contain cell material substantially ", wherein, some cellular components in its cell of polypeptide and natural or recombinant production is separated.In one embodiment, this saying comprises such polypeptide prepared product of the present invention " not contain cell material substantially ": it has and is less than about 30% (with dry weight) contaminative polypeptide, contaminative polypeptide more preferably less than about 20%, further, most preferably be less than about 5% contaminative polypeptide more preferably less than about 10% contaminative polypeptide.

Mensuration to enzymic activity and kinetic parameter is very ripe in the art.The active experiment that is used to measure any given enzyme through transforming must be customized at the given activity of wild-type enzyme, and this is in those skilled in the art's limit of power.Summary about enzyme is general, and describing in detail about structure, kinetics, principle, method, application and the example of measuring a lot of enzymic activitys has a lot, and they are well known to a person skilled in the art.

The invention still further relates to the method for producing the transgenic plant comprise at least a polynucleotide that table 1 lists; wherein; the expression of described polynucleotide in plant causes: than the wild-type kind of plant; plant grows under normal or water confined condition and/or output increases; and/or the tolerance of environment-stress increased; described method comprises the steps: that (a) introduces the expression vector that comprises the listed at least a polynucleotide of table 1 in vegetable cell; and the transgenic plant that (b) produce the described polynucleotide of expression from vegetable cell; wherein; the expression of polynucleotide in transgenic plant causes: than the wild-type kind of plant; plant grows under normal or water confined condition and/or output increases, and/or the tolerance of environment-stress is increased.Plant can be, but is not limited to, and protoplastis, gamete are produced cell and be regenerated as the cell of whole strain plant.When using in this article, " transgenosis " refers to contain any plant, vegetable cell, callus, plant tissue or the plant part of at least a recombination of polynucleotide that table 1 lists.In a lot of situations, recombination of polynucleotide is stabilized and is integrated into karyomit(e) or stable extra-chromosomal element, makes it pass to the follow-up generation.

The present invention also provides under normal or water confined condition to be increased plant-growth and/or output and/or increases the method for plant to the tolerance of environment-stress, and described method comprises the steps: the expression of at least a polynucleotide that in plant increase table 1 is listed.Can increase proteic expression by any method known to those skilled in the art.

Can analyze growth characteristics and/or the metabolism of plant then by under normal and/or more inappropriate condition, cultivating modified plant, assess the influence of genetic modification plant-growth and/or output and/or stress tolerance.This type of analytical technology is well known to a person skilled in the art, it comprises that dry weight, weight in wet base, polypeptide are synthetic, carbohydrate is synthetic, lipid is synthetic, evapotranspiration speed, general plant and/or crop yield, bloom, breed, set seeds (seed setting), root growth, respiratory rate, photosynthetic rate, metabolism composition etc., wherein uses biological technical field method known to the skilled to carry out.

Further set forth the present invention by following embodiment, described embodiment should be interpreted as by any way that scope of the present invention is applied restriction.

Embodiment 1

Full Length cDNA Cloning

The full length DNA sequence (SEQ ID NO:43) of small liwan moss (Physcomitrella patens) EST65 methionine sulphoxide reductase enzyme family protein is e in the e value^-10Level on canola oil dish (canola), soybean, paddy rice, corn, Semen Lini, Sunflower Receptacle, barley and wheat cDNA patent database comparison (Altschul etc., 1997, Nucleic Acids Res.25:3389-3402).Analyze all contigs for the full length sequence of supposition and hit, and the longest clone of the total length contig of representative supposition is checked order fully.The homologue in a canola oil dish source, the homologue in two paddy rice sources, the homologue and a wheat source place homologue in a soybean source are identified.These sequences are with the amino acid identity of nearest known common sequence and the degree of similarity are listed in table 2 to 6 (Align 2.0) separately.

Table 2

The comparison of BN51364980 (SEQ ID NO:2) and known methionine sulphoxide reductase enzyme

The public database accession number	Species	Sequence homology (%)
			??NP_564640	Arabidopis thaliana	??82.00％
??AAM65202	Arabidopis thaliana	??66.70％
			??BAD35399	Rice	??56.10％
??NP_001057620	Rice	??55.80％
			??ZP_01592095	Mud bacterium (Geobacter lovleyi SZ)	??42.40％

Table 3

The comparison of OS34096188 (SEQ ID NO:4) and known methionine sulphoxide reductase enzyme

The public database accession number	Species	Sequence homology (%)
			??ABE84787	Puncture vine clover (Medicago truncatula)	??72.60％
??NP_567639	Arabidopis thaliana	??61.90％

The public database accession number	Species	Sequence homology (%)
			??AAM62876	Arabidopis thaliana	??61.00％
??NP_567271	Arabidopis thaliana	??58.70％
			??EAY98001	Rice	??57.80％

Table 4

The comparison of OS32583643 (SEQ ID NO:6) and known methionine sulphoxide reductase enzyme

The public database accession number	Species	Sequence homology (%)
			??NP_001057620	Rice	??99.10％
??BAD35399	Rice	??74.80％
			??NP_564640	Arabidopis thaliana	??59.60％
??AAM65202	Arabidopis thaliana	??53.70％
			??YP_846684	??Syntrophobacter?fumaroxidans?MPOB	??44.20％

Table 5

The comparison of GM53626178 (SEQ ID NO:8) and known methionine sulphoxide reductase enzyme

The public database accession number	Species	Sequence homology (%)
			??ABE84787	The puncture vine clover	??72.80％
??NP_567639	Arabidopis thaliana	??60.90％
			??AAM62876	Arabidopis thaliana	??60.90％
??EAY98001	Rice	??59.10％
			??AAO72582	Rice	??57.60％

Table 6

The comparison of TA56540264 (SEQ ID NO:10) and known methionine sulphoxide reductase enzyme

The public database accession number	Species	Sequence homology (%)
			??EAY98001	Rice	??72.90％
??NP_001055501	Rice	??69.50％
			??ABE84787	The puncture vine clover	??65.50％
??NP_567639	Arabidopis thaliana	??60.60％
			??AAM62876	Arabidopis thaliana	??59.60％

The full length DNA sequence (SEQ IDNO:44) of small liwan moss EST12 homeodomain leucine zipper protein matter is e in the e value^-10Level on canola oil dish, soybean, paddy rice, corn, Semen Lini, Sunflower Receptacle, barley and wheat cDNA patent database comparison (Altschul etc., 1997, NucleicAcids Res.25:3389-3402).Analyze all contigs for the full length sequence of supposition and hit, and the longest clone of the total length contig of representative supposition is checked order fully.The homologue in a canola oil dish source, the homologue in the homologue in a soybean source and a wheat source is identified.These sequences are with the amino acid identity of nearest known common sequence and the degree of similarity are listed in table 7 to 9 (Align 2.0) separately.

Table 7

The comparison of BN45206322 (SEQ ID NO:12) and known homeodomain leucine zipper protein matter

The public database accession number	Species	Sequence homology (%)
			??AAR04932	Colea	??95.20％
??AAF73482	Turnip (Brassica rapa)	??91.30％
			??AAD41726	Arabidopis thaliana	??81.20％
??NP_195716	Arabidopis thaliana	??69.90％
			??AAK96762	Arabidopis thaliana	??69.60％

Table 8

The comparison of GM48923793 (SEQ ID NO:14) and known homeodomain leucine zipper protein matter

The public database accession number	Species	Sequence homology (%)
			??AAX98670	Soybean	??56.00％
??AAK84886	Kidney bean (Phaseolus vulgaris)	??51.60％

The public database accession number	Species	Sequence homology (%)
			??CAA64417	Tomato (Solanum lycopersicum)	??47.40％
??BAA05624	Radix Dauci Sativae (Daucus carota)	??46.70％
			??AAF01765	Soybean	??44.00％

Table 9

The comparison of TA55969932 (SEQ ID NO:16) and known homeodomain leucine zipper protein matter

The public database accession number	Species	Sequence homology (%)
			??NP_001048008	Rice	??74.00％
??EAY87390	Rice	??73.80％
			??NP_001061807	Rice	??49.90％
??AAD37698	Rice	??49.60％
			??AAS83417	Rice	??46.30％

The full length DNA sequence (SEQ ID NO:45) that small liwan moss EST307 contains the zinc finger protein matter of A20 and AN1 structural domain is e in the e value^-10Level on canola oil dish, soybean, paddy rice, corn, Semen Lini, Sunflower Receptacle, barley and wheat cDNA patent database comparison (Altschul etc., 1997, Nucleic Acids Res.25:3389-3402).Analyze all contigs for the full length sequence of supposition and hit, and the longest clone of the total length contig of representative supposition is checked order fully.The homologue in two canola oil dish sources, the homologue in a barley source, the homologue in two corn sources, the homologue in two Semen Lini sources, the homologue in three soybean sources, the homologue in the homologue in three paddy rice sources and a wheat source is identified.These sequences are with the amino acid identity of nearest known common sequence and the degree of similarity are listed in table 10 to 22 (Align 2.0) separately.

Table 10

BN47310186 (SEQ ID NO:18) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??NP_564585	Arabidopis thaliana	??88.70％
??AAN71995	Arabidopis thaliana	??88.10％
			??ABL67658	Buddha's hand (Citrus cv.Shiranuhi)	??59.40％

The public database accession number	Species	Sequence homology (%)
			??AAQ84334	Rice	??56.00％
??AAD38146	Apricot (Prunus armeniaca)	??55.20％

Table 11

BN51359456 (SEQ ID NO:20) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??NP_190848	Arabidopis thaliana	??71.60％
??AAK68811	Arabidopis thaliana	??71.10％
			??NP_565844	Arabidopis thaliana	??66.00％
??ABE93196	The puncture vine clover	??51.10％
			??AAN71995	Arabidopis thaliana	??47.10％

Table 12

HV62552639 (SEQ ID NO:22) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??NP_001055132	Rice	??70.10％
??AAR96005	Fruitlet musa acuminata (Musa acuminata)	??51.80％
			??AAA33773	Kidney bean	??42.60％
??EAZ09556	Rice	??40.80％
			??EAZ45178	Rice	??39.40％

Table 13

ZM61995511 (SEQ ID NO:24) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??AAQ84334	Rice	??79.50％

The public database accession number	Species	Sequence homology (%)
			??AAX14637	Corn	??77.00％
??EAZ01657	Rice	??71.70％
			??ABL67658	Buddha's hand	??69.50％
??NP_001046186	Rice	??65.90％

Table 14

LU61567101 (SEQ ID NO:26) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??CAE73100	Nematode (Caenorhabditis briggsae)	??29.30％
??NP_190848	Arabidopis thaliana	??29.20％
			??XP_001357850	Fruit bat (Drosophila pseudoobscura)	??29.20％
??EAY92150	Rice	??29.10％
			??ABL97956	Turnip	??28.90％

Table 15

LU61893412 (SEQ ID NO:28) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??ABL67658	Buddha's hand	??67.40％
??AAD38146	Apricot	??64.60％
			??AAQ84334	Rice	??61.70％
??ABN08135	The puncture vine clover	??61.50％
			??AAN71995	Arabidopis thaliana	??61.00％

Table 16

OS39781852 (SEQ ID NO:30) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??EAZ45178	Rice	??99.40％
??EAZ09556	Rice	??99.40％
			??NP_001063521	Rice	??65.90％
??ABI23728	Chrysanthemum (Chrysanthemum x morifolium) on the desk	??58.80％
			??AAA33773	Kidney bean	??47.30％

Table 17

OS34701560 (SEQ ID NO:32) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??NP_565844	Arabidopis thaliana	??58.80％
??ABE93196	The puncture vine clover	??56.40％
			??NP_190848	Arabidopis thaliana	??55.60％
??AAK68811	Arabidopis thaliana	??55.60％
			??ABL67658	Buddha's hand	??48.30％

Table 18

OS36821256 (SEQ ID NO:34) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??EAZ45178	Rice	??63.00％
??EAZ09556	Rice	??63.00％
			??ABI23728	Chrysanthemum on the desk	??51.10％
??AAA33773	Kidney bean	??43.40％
			??AAX14637	Corn	??43.30％

Table 19

GM51659494 (SEQ ID NO:36) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??AAA33773	Kidney bean	??58.00％
??EAZ09556	Rice	??54.20％
			??EAZ45178	Rice	??54.20％
??NP_566429	Arabidopis thaliana	??51.50％
			??ABI23728	Chrysanthemum on the desk	??44.40％

Table 20

GM49780101 (SEQ ID NO:38) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??ABN08135	The puncture vine clover	??76.70％
??ABL67658	Buddha's hand	??66.30％
			??AAD38146	Apricot	??64.60％
??AAQ84334	Rice	??64.40％
			??AAX14637	Corn	??61.90％

Table 21

GM59637305 (SEQ ID NO:40) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??AAD38146	Apricot	??69.30％
??ABL67658	Buddha's hand	??68.60％
			??AAQ84334	Rice	??65.90％
??AAX14637	Corn	??64.00％
			??NP_564585	Arabidopis thaliana	??63.70％

Table 22

TA55974113 (SEQ ID NO:42) and the known comparison that contains the zinc finger protein matter of A20 and AN1 structural domain

The public database accession number	Species	Sequence homology (%)
			??EAZ09556	Rice	??73.20％
??EAZ45178	Rice	??72.60％
			??ABI23728	Chrysanthemum on the desk	??54.20％
??NP_001063521	Rice	??49.00％
			??AAA33773	Kidney bean	??46.10％

Embodiment 2

The Arabidopis thaliana plant of water stress-tolerance

Polynucleotide in the table 1 is connected in the binary vector that contains selected marker.The recombinant vectors of Xing Chenging is included in the gene of the corresponding sense orientation under the promotor control of composition like this.This recombinant vectors is transformed in the agrobacterium tumefaciens system according to the condition of standard.Environmental Col-0 of Arabidopis thaliana or C24 are according to standard conditions plantation and conversion.The screening of T1 and T2 plant have that marker gene optionally gives to the resistance of reagent optionally.The T3 seed is used in the experiment of greenhouse or growth room.

Before plantation approximately 3-5 days, with seed refrigeration in order to layering.With the seed plantation, apply fertilizer then, and keep humidity with transparent cupola.For the mensuration of " biomass ", plant is planted in the greenhouse at 22 ℃, and the photoperiod is 8 hours dark of illumination in 16 hours, and a week waters twice.For the mensuration of " periodically arid ", it is that 22 ℃ of relative humidity are that the photoperiod is set at 8 hours dark of illumination in 16 hours, waters at after planting 0 day, 18 days and 25 days in 55% the growth room that plant is planted in temperature.

At the 19th and 22 day, the plant area of every strain plant, the leaf area, biomass, color distribution, colour intensity and growth velocity can be measured with commercially available imaging system.The calculating of biomass is the total area of the plant leaf of last Measuring Time point.The calculating of growth velocity is again divided by the area of the plant leaf of the time point of first measurement behind the area of plant leaf of the area of plant leaf of the time point of last measurement time point that deducts first measurement.The calculating of health index is the total area of the area of deep green leaf divided by the plant leaf.Table 23 has presented to cross has expressed SEQ ID NO:5, biomass, growth velocity and the health index of the independent transformation event of the transfer-gen plant of 7 and 13 polynucleotide (strain system).Calculate the variation per-cent of a system, check the value (p value) of calculating significance with t with respect to the contrast of blended wild-type.If this per-cent change greater than 0 and the p value less than 0.1, this is to be considered to male, if the p value is greater than 0.1, how many variations of IOP irrespective of percentage is, then be considered to not have significance (NS), if per-cent change less than 0 and p value less than 0.1, then be considered to feminine gender.

Table 23

Embodiment 3: the Arabidopsis plant of nitrogen stress tolerance

But the polynucleotide of table 1 are connected the binary vector that into contains selective marker.The recombinant vectors that obtains contain the justice that is under the constitutive promoter to corresponding gene.According to standard conditions recombinant vectors is transformed into agrobacterium tumefaciens bacterial strain.Cultivate and environmental Col-0 of arabidopsis thaliana transformation or C24 according to standard conditions.But, screen T1 and T2 plant at the resistance that selectable marker gene is given to selective agent.

Use the substrate (substrate) do not contain the machine component, with plant-growth on platform (flat).Every platform water is got wet, will the seedling culture transferring of resistance be arranged to substrate to selective agent then.Be set to that 22 ℃, relative humidity are 55%, periodicity of illumination is set to culturing plants in hour dark growth room, 16 hours illumination/8.In supplying water, added controlled low or high nitrogen nutrition solution at the 12nd, 15,22 and 29 day.Gave the water supply of nonnutritive solution at the 18th, 25 and 32 day.Use commercial obtainable imaging system,, gather the image that pallet (tray) is gone up all plants at the 26th, 30 and 33 day.At each imaging time point, metering needle comprises plant area, leaf area, biomass, color distribution, colour intensity and growth velocity to biomass and the plant phenotype of every strain plant.

Embodiment 4

The canola oil dish plant of resistance of reverse

With 4 day age canola oil dish seedling the cotyledon petiole as explant, be used for tissue culture, and transform according to EP1566443.Commercial cultivar Westar (Agriculture Canada) is the standard variety that is used to transform, but also can use other kind.The agrobacterium tumefaciens GV3101:pMP90RK that contains binary vector is used to the canola oil dish and transforms.The standard binary vector that is used to transform is pSUN (WO02/00900), but also described be used for Plant Transformation a lot of different binary vector systems (for example, An, G. is at Agrobacterium Protocols, Methods inMolecularBiology volume 44, the 47-62 page or leaf, Gartland KMA and MR Davey write, Humana Press, Totowa, New Jersey).The plant promotor that the plant expression casette that uses comprises a selected marker and the cDNA of the polynucleotide of regulating and control to encode transcribes.Can use the multiple choices marker gene, comprise United States Patent(USP) Nos. 5,767,366 and 6,225, the acetohydroxy acid synthase of 105 disclosed sudden changes (AHAS) gene.With suitable promoter regulation character gene, so that composing type, growth, tissue or the environment conditioning to genetic transcription to be provided.

With canola oil colza surface sterilization 2 minutes in 70% ethanol, in 55 ℃ of warm tap water, hatched 15 minutes, in 1.5% clorox, hatched 10 minutes then, then with the distilled water rinsing of sterilization three times.Then seed being placed does not have on the MS of the hormone substratum, and this substratum contains Gamborg B5 VITAMIN, 3% sucrose and 0.8%Oxoidagar.Shine (＜50 μ Mol/m at 24 ℃ of low lights²S, illumination in 16 hours) order seed germination 4 days.Downcut the cotyledon petiole explant that is connected with cotyledon from external seedling, immerse bacterial suspension by cut ends and come to inoculate with Agrobacterium with petiole explant.Then at 24 ℃, under the illumination in 16 hours, explant was cultivated 3 days comprising on the MS substratum of VITAMIN, contain BAP, 3% sucrose, 0.5g/l MES (pH5.2), 0.5mg/l GA3, the 0.8%Oxoidagar of 3.75mg/l in the substratum.After cultivating altogether 3 days with Agrobacterium, petiole explant is forwarded on the regeneration culture medium that contains 3.75mg/l BAP, 0.5mg/l GA3,0.5g/l MES (pH 5.2), 300mg/l Ticarcillin/Clavulanate Acid and selective agent, regenerate up to branch.The branch in case explant germinates, just it is transferred to branch and prolong substratum (A6, the MS substratum that contains complete intensity, it comprises VITAMIN, 2% sucrose, 0.5% Oxoidagar, 100mg/l inositol, 40mg/l adenine sulfate, 0.5g/l MES (pH 5.8), 0.0025mg/l BAP, 0.1mg/l IBA, 300mg/l Ticarcillin/Clavulanate Acid and selective agent) on.

Use the TaqMan probe,, analyze from just for the sample of the external and greenhouse materials of transgenic plant (T0) by qPCR, with existing of checking T-DNA, and the quantity of measuring the T-DNA integration.

By self-pollination from just producing seed for transgenic plant.With s-generation plant culturing under greenhouse experiment, and self-pollination.Use the TaqMan probe to analyze plant,, and measure the quantity that T-DNA integrates with existing of checking T-DNA by qPCR.Relatively the isozygoty stress tolerance of transgenic plant, heterozygosis transgenic plant and unisexuality (azygous) (inefficacy transgenosis) plant for example, inembodiment 2 and 3 described similar checks, is also studied among both relatively their output in greenhouse and field.

Embodiment 5: screening stress tolerance rice plant

Use known method to produce the transgenosis rice plant of the polynucleotide that comprise table 1.Produce about 15 to 20 independent transformants (T0).To just transfer to the greenhouse from tissue culture room, be used for cultivating and results T1 seed for transformant.At genetically modified existence/do not exist 5 incidents to be retained with 3: 1 isolating T1 offsprings.For in these incidents each, by the visual indicia screening, the 10 strain T1 seedling of selecting to contain 10 strain T1 seedling of transgenosis (heterozygosis and homozygote) and lacking transgenosis (inefficacy zygote (nullizygotes)).Forward the T1 plant of selecting to greenhouse.Every strain plant obtains unique bar code label, so that phenotypic data and corresponding plant are clearly connected.Be provided with down at following environment, on the soil of T1 plant culturing in 10cm diameter basin of selecting: photoperiod=11.5 hour, day light intensity=30,000lux or higher, daytime temperature=28 ℃ or higher, nocturnal temperature=22 ℃, relative humidity=60-70%.Transgenic plant and corresponding inefficacy zygote are planted side by side with random site.From sowing stage, till the stage of maturity, make plant for several times by the digital imagery case.Each time point is gathered the image (2048x1536 pixel, 1,600 ten thousand looks) of every strain plant with at least 6 different angles.

In experiment for the second time, verify the data that obtain in the experiment of carrying out with the T1 plant first time with the T2 plant.Selected being used for of strain with correct expression pattern further analyzed.Express by monitoring mark, screening is from the seed of sun plant among the T1 (heterozygote and homozygote) batch.For each incident of selecting, keep the seed batch of heterozygote again, be used for the T2 assessment.In every batch of seed batch, in the greenhouse, cultivate the positive of same quantity and heliophobous plant to be used for assessment.

At growth and/or the output and/or the stress tolerance of its improvement, the screening transgenic plant for example, are usedembodiment 2 and 3 similar tests of describing, and for output, greenhouse and field are studied among both and all carried out.

Embodiment 6: the soybean plants of stress tolerance

Use the method for describing among the total common unsettled international application no WO 2005/121345, the polynucleotide of table 1 are transformed into soybean, the content of the document is incorporated this paper by reference into.

At its growth that improves under the water confined condition and/or arid, salt and/or cold tolerance, the screening transgenic plant for example, are usedembodiment 2 and 3 similar tests of describing, and screen at output in greenhouse and field research then.

Embodiment 7: the wheat plant of stress tolerance

Use people such as Ishida, 1996, the described method of Nature Biotech.14745-50 changes the polynucleotide of table 1 over to wheat.Jejune embryo and the agrobacterium tumefaciens that carries " super double base " carrier are cultivated altogether, transgenic plant are taken place to reclaim by organ.This flow process provides 2.5% to 20% transformation efficiency.Screen transgenic plant at its growth that improves and/or output and/or stress tolerance then under the water confined condition, for example, useembodiment 2 and 3 similar tests of describing, for output, greenhouse and field are studied among both and are all carried out.

Embodiment 8: the maize plant of stress tolerance

Use Agrobacterium that the polynucleotide of table 1 are transformed the into immature embryos of corn.Behind imbibition (imbibition), embryo is forwarded to the substratum that does not have selective agent.After 7 to 10 days, embryo is forwarded on the substratum that contains selective agent, cultivated for 4 weeks (2 weeks shifted once), to obtain callus cell through transforming.By being forwarded to, resistant calli starts plant regeneration on the substratum that is supplemented with selective agent and two to three weeks of cultivation under 25-27 ℃ of illumination.Then the regenerated branch is forwarded on the box of taking root with the substratum that contains selective agent.The plantlet that will have a root forwards in the potting mixtures in the medium and small basin in greenhouse, after shaking down with its culture transferring in bigger basin, and in the greenhouse, keep, up to maturation.

To the every strain in these plants all unique label, sampling and analyzed at the transgenosis copy number.Mark transgenic positive and heliophobous plant,, be used for together culture transferring to big basin with the pairing of identical size.This provides the also competitive environment of unification for transgenic positive and heliophobous plant.Big basin is watered, reach certain water-retaining quantity among field of soil per-cent, this depends on the seriousness that the water of needs is coerced.Keep soil water level by watering every other day.Measure plant-growth and physiological character at growing period, for example height, diameter stem, leaf roll song, plant wilt, leaf extend speed, leaf water state, chlorophyll content and photosynthetic rate.After vegetative period, the over-ground part of results plant claims fresh weight and dry weight to every strain plant.Then the drought tolerance phenotype between transgenic positive and the heliophobous plant is compared.

Basin is added a cover, and described lid allows that seedling is long to get up but make the minimization of loss of water.Regularly every basin is weighed, added water to keep initial water-content.At the experiment end, measure fresh weight and the dry weight of every strain plant, calculate the water of every strain plant consumption, calculate the WUE of every strain plant.Measure plant-growth and physiological character at experimental session, for example, WUE, highly, diameter stem, leaf roll song, plant wilt, leaf extend speed, leaf water state, chlorophyll content and photosynthetic rate.Then the WUE phenotype between transgenic positive and the heliophobous plant is compared.

These basins are remained in the zone that unified envrionment conditions is arranged in the greenhouse, and optimum the cultivation.To the every strain in these plants all unique label, sampling and analyzed at the transgenosis copy number.Make plant grow under these conditions, reach predetermined growth phase up to them.Stop feedwater then.Along with the increase of coercive intensity, measure plant-growth and physiological character, for example, highly, diameter stem, leaf roll song, plant wilt, leaf extend speed, leaf water state, chlorophyll content and photosynthetic rate.Then the dry tolerogenic phenotype between transgenic positive and the heliophobous plant is compared.

Plantation is at the isolating transgenic corn seed of transformation event, to test in the check of cyclicity arid in little basin.These basins are remained in the zone that unified envrionment conditions is arranged in the greenhouse, and optimum the cultivation.To the every strain in these plants all unique label, sampling and analyzed at the transgenosis copy number.Make plant grow under these conditions, reach predetermined growth phase up to them.With the regular time interval, plant is repeated to water to saturated then.Repeat this water/arid circulation at experimental session.During growth phase, measure plant-growth and physiological character, for example, highly, diameter stem, leaf roll song, leaf extend speed, leaf water state, chlorophyll content and photosynthetic rate.At the experiment end, results plant, the fresh weight and the dry weight of title over-ground part.Then the cyclicity drought tolerance phenotype between transgenic positive and the heliophobous plant is compared.

For testing the drought tolerance of isolating transgenic corns under no rain condition, use the managed drought stress of single position or a plurality of positions.Irrigate by drip cloth (dip tape) and top and to control crop water utilizability, this carries out in following position, this position has in average 5 months season less than the precipitation of 10cm and the minimum temperature above 5 ℃, or such position, its quilt with expectation automatically " anti-canopy (rain out shelter) " stop should the season rainfall, anti-canopy is recalled unwanted the time so that open field condition to be provided.Follow the standard agricultural practice in the zone, carry out soil preparation, plantation, fertilising and insect control.Insert the existence of incident and isolating seed at every fritter upper seeding wheel at single transgenosis.On the leaf sample, use the check of Taqman transgenosis copy number, so that transgenosis and inefficacy segregant control plant are distinguished.Also at a series of phenotypes relevant, the plant of gene type is by this way scored with drought tolerance, growth and output.These phenotypes comprise the spike number of the Number of kernels of the grain weight of plant height, every plant, every plant, every plant, on the ground dry weight, leaf be to conductivity, the leaf CO of water vapor₂The optical absorbance of the chlorophyll fluorescence parameters that the chlorophyll content, photosynthesis of picked-up, leaf is relevant, water use efficiency, the flow of water of leaf, the relative water-content of leaf, stem liquid flowing rate, stem hydraulic conductivity, leaf temp, foliage reflectance, leaf, leaf area, extend speed, leaf angle, leaf roll song and survive to the required fate of blooming, interval of blooming-reel off raw silk from cocoons, grain milk time length, osmotic potential, osmoregulation, root size, leaf.The standard scheme all be to use manufacturer to provide is provided for all, is used for that the field obtainable equipment of physiological commerce carries out.Each plant is used as the repeating unit of every kind of incident.

For testing the drought tolerance that no rain condition is descended non-separation transgenic corns, use the managed drought stress of single position or a plurality of positions.Irrigate by drip cloth and top and to control crop water availability, this carries out in following position, this position has in average 5 months season less than the quantity of precipitation of 10cm and the minimum temperature above 5 ℃, or such position, its quilt with expectation automatically " anti-canopy " stop should season quantity of precipitation, anti-canopy is recalled unwanted the time so that open field condition to be provided.Follow the standard agricultural practice in the zone, carry out soil preparation, plantation, fertilising and insect control.The design experiment scheme, it is paired in abutting connection with splat to make that the splat contain non-separation transgenic event and inefficacy segregant contrast.The inefficacy segregant is because Mendelian separates the offspring's (perhaps coming from offspring's strain) who does not contain genetically modified transgenic plant.Around test, be distributed with and additionally repeat paired splat at particular event.In paired splat, a series of phenotypes relevant with drought tolerance, growth and output are scored, and on the level of splat, it is assessed.When measuring technology only can be applied to bion, in splat, select plant at random at every turn.These phenotypes comprise the spike number of the Number of kernels of the grain weight of plant height, every plant, every plant, every plant, on the ground dry weight, leaf be to conductivity, the leaf CO of water vapor₂The optical absorbance of the chlorophyll fluorescence parameters that the chlorophyll content, photosynthesis of picked-up, leaf is relevant, water use efficiency, the flow of water of leaf, the relative water-content of leaf, stem liquid flowing rate, stem hydraulic conductivity, leaf temp, foliage reflectance, leaf, leaf area, extend speed, leaf angle, leaf roll song and survive to the required fate of blooming, interval of blooming-reel off raw silk from cocoons, grain milk time length, osmotic potential, osmoregulation, root size, leaf.The standard scheme all be to use manufacturer to provide is provided for all, is used for that the field obtainable equipment of physiological commerce carries out.Each splat is used as the repeating unit of every kind of incident.

For transgenic corns being carried out the multi-position test, select 5 to 20 positions that comprise main corn planting district at drought tolerance and output.With its extensive distribution, so that the crop water availability (based on medial temperature, humidity, quantity of precipitation and soil type) of a series of expectations to be provided.Not to the in addition outer change of standard agricultural practice of crop water availability.The design experiment scheme, it is paired in abutting connection with splat to make that the splat contain non-separation transgenic event and inefficacy segregant contrast.In paired splat, a series of phenotypes relevant with drought tolerance, growth and output are scored, and on the level of splat, it is assessed.When measuring technology only can be applied to bion, in splat, select plant at random at every turn.These phenotypes comprise the spike number of the Number of kernels of the grain weight of plant height, every plant, every plant, every plant, on the ground dry weight, leaf be to conductivity, the leaf CO of water vapor₂The optical absorbance of the chlorophyll fluorescence parameters that the chlorophyll content, photosynthesis of picked-up, leaf is relevant, water use efficiency, the flow of water of leaf, the relative water-content of leaf, stem liquid flowing rate, stem hydraulic conductivity, leaf temp, foliage reflectance, leaf, leaf area, extend speed, leaf angle, leaf roll song and survive to the required fate of blooming, interval of blooming-reel off raw silk from cocoons, grain milk time length, osmotic potential, osmoregulation, root size, leaf.The standard scheme all be to use manufacturer to provide is provided for all, is used for that the field obtainable equipment of physiological commerce carries out.Each splat is used as the repeating unit of every kind of incident.

Appendix

Come from the cDNA sequence (SEQ ID NO:1) of the BN51364980 of canola oil dish:

atggcttcttctagttgtttcaccattcagtcacgtttcgtctcagcgagaacaaagctcgattcaatctccaaaccgagtctctccggattcgcttgtcgttctcttacaaaacccagaaacttgaatctctctgttcttcttcggtgttccatgggttcctttaactcttctcagaaatcagacaacgtccaagaagctgcaaagagtgactttgcttcaataagtgaaggtgagtggaagaaacggctaacaccagaacagtattacatcaccagacagaagggaacagagagagctttcactggtgagtattggaatacaaagaccccaggagtatacaaatgtatctgttgcgacacgccactgtttgactcatcaacaaagcttgatagtggaaccgggtggccatcgtattaccaacctattggaaacaatgtgaagtcaaagctggacctctctatcatcttcatgcctagacaagaagttatctgtgctgtttgtaacgcccatcttggtcatgtcttcgatgacggtccacgaccaaccggaaaacgatattgcctcaacagtgctgctctgaaacttgagtcattggag?agaacaagagaatga

BN51364980 cDNA is translated as following amino acid sequences (SEQ ID NO:2):

massscftiqsrfvsartkldsiskpslsgfacrsltkprnlnlsvllrcsmgsfnssqksdnvqeaaksdfasisegewkkrltpeqyyitrqkgteraftgeywntktpgvykciccdtplfdsstkldsgtgwpsyyqpignnvkskldlsiifmprqevicavcnahlghvfddgprptgkryclnsaalkleslertre

Come from the cDNA sequence (SEQ ID NO:3) of the OS34096188 of paddy rice:

atgggcttcaatattctgagaaccacttccatctccactcctatctcttcctccaaatccaaacccattttctcaactcttcttcgttcttctccttccaccattttccccccaaagtccgttactcccaccactcttttcgtttctgccacccccttcttcactctccatcccaagcttggttttcgtggtgggattgtggccatggccgcacctggctctctccgcaaatccgaggaagagtggcgcgcaattctctcccctgaacagtttcggatcctcaggcaaaagggcaccgagttccctggaacaggagagtatgacaagttctatgaagagggagtttacaactgtgctggttgtgggactccactctacaggtccataacaaaattcaattctggttgtggctggccagccttctatgaggggattcccggagccataaatcgcaatccggatcctgatgggatgaggacagaaataacgtgtgctgcttgtgggggacatctaggtcacgtctttaaaggagaaggatttccaacacccactaacgaacgccattgtgtcaatagcatttcgctgaaatttgcgccagccaattcttattcttaa

OS34096188 cDNA translates into following amino acid sequences (SEQ ID NO:4):

mgfnilrttsistpissskskpifstllrsspstifppksvtpttlfvsatpfftlhpklgfrggivamaapgslrkseeewrailspeqfrilrqkgtefpgtgeydkfyeegvyncagcgtplyrsitkfnsgcgwpafyegipgainrnpdpdgmrteitcaacgghlghvfkgegfptptnerhcvnsislkfapansys

Come from the OS32583643 cDNA sequence (SEQ ID NO:5) of paddy rice:

atggccatgcggcaatacgcggctgctaccgctgcctcctccagtttcagagcacgtccacgggcgcgcccctcctgcctcccagccgccgccctgcccttggcgccttgctgtggtgtggcgtggagccgtgctagctacaggcgagcctccgttcgtgccatgggtgccgcttcatcgtcttcgtcgtcgtcgtcgtcgtctccgtcgccgcagggtcaagcccaagcccaagcccaaggtaaaccgaactacagtacatctctgactgatgaggagtggaggaagcgcctgacaaaagatcagtattacattactcggcagaagggcacagaaagagcatttactggggaatactggaacaccaaaaccccgggcatctaccattgtgtctgctgtgacacccctctttttgagtcatcgaccaaatttgatagtggtactgggtggccgtcatattatcaacccattggagataatgtaaagtgcaagcttgatatgtccatcatattcatgcctcggactgaggtgctgtgtgctgtctgtgacgctcatctggggcacgtgtttgatgatgggccacgaccaacagggaaaagatactgtatcaatagcgcatctctcaagctgaagaagacccagtag

OS32583643 cDNA translates into following amino acid sequences (SEQ ID NO:6):

mamrqyaaataasssfrarprarpsclpaaalplapccgvawsrasyrrasvramgaasssssssssspspqgqaqaqaqgkpnystsltdeewrkrltkdqyyitrqkgteraftgeywntktpgiyhcvccdtplfesstkfdsgtgwpsyyqpigdnvkckldmsiifmprtevlcavcdahlghvfddgprptgkrycinsaslklkktq

Come from the GM53626178 cDNA sequence (SEQ ID NO:7) of soybean:

atgggattgagtattctgagaagcacttccatttccactcctatctcttcctccaaatccaaacccattttctcaactcttgttcgttcatctttcgcctccatttcccccacaaagtgtgttactcccaccactcttttcgtttctgccacccccttcttcaccgcctcacccaagcgcggctttcgtggtgggattgtggccatggccgccgctggctcgctccgcaaatcagaggaagagtggcgcgcagttctctcccctgaacagtttcgtattctcaggcaaaagggcaccgagttccctggaacaggagagtatgacaagttctttgatgagggagtttacaactgtgctggttgtgggacacctctctacaggtccttaacaaaattcaattctggttgtggctggccagccttctatgaggggattcctggagccataaatcgcaatccggaccctgatgggatgaggacagaaataacgtgtgctgcttgtgggggacatctaggtcacgtctttaaaggagaaggatttccaacgcccactaacgaacgccattgtgtcaatagcatttcactgaaatttgcgccagccaattcttaa

GM53626178 cDNA translates into following amino acid sequences (SEQ ID NO:8)

mglsilrstsistpissskskpifstlvrssfasisptkcvtpttlfvsatpfftaspkrgfrggivamaaagslrkseeewravlspeqfrilrqkgtefpgtgeydkffdegvyncagcgtplyrsltkfnsgcgwpafyegipgainrnpdpdgmrteitcaacgghlghvfkgegfptptnerhcvnsislkfapans

Come from the TA56540264 cDNA sequence (SEQ ID NO:9) of wheat:

atggcgtcgccccacgcccacccggccacgcggcccctctcatcgctcccgtccctcctcctcgcccgctcctcctccgccgccaccgccgccgcgtcgtccgcccgccccgcctccctctccctctcgtgctcgcggtcgcgggcgcgggcctactgcccagccggacgacggttgccgggcgccgtggtggctatgtcgtcggcggcgcccacgccggggcccgtgcagaagtcggaggaggagtgggaggccgtcctcacgccggagcagttccgcatcctccgccgcaagggcaccgagtatcctggaacaggtgaatatgacaagttcttcagtgagggtatttacggatgtgctggctgtggaacccccttgtacaaatcatctacgaagttcaactcagggtgtggttggccagcattctatgaaggatttcctggagccataaaacggacggcggatcctgatgggaggcgaattgagatcacatgtgctgcttgtgaaggacatctggggcatgtgttcaaaggggaggggttcaacacgccgactgatgagcgacactgcgtcaacagtatctcactcaagttcgttccggcctctgaagaggctagttga

TA56540264 cDNA translates into following amino acid sequences (SEQ ID NO:10):

masphahpatrplsslpslllarsssaataaassarpaslslscsrsraraycpagrrlpgavvamssaaptpgpvqkseeeweavltpeqfrilrrkgteypgtgeydkffsegiygcagcgtplyksstkfnsgcgwpafyegfpgaikrtadpdgrrieitcaaceghlghvfkgegfntptderhcvnsislkfvpaseeas

Come from the BN45206322 cDNA sequence (SEQ ID NO:11) of canola oil dish:

atgatgaagagattaagcagttcagattcagtgggtggtctcatctctttatgtcccactacttccacagatcagccgaatccaagaagatgcgggagagaatttcagtcgatgctcgaaggttacgaggaggaagaagaagaagccataaccgaggaaagaggacaaaccggtttagccgagaagaagagacggttaaacattaaccaagttaaagccttggagaaaaatttcgagttagagaacaagcttgagcctgagaggaaagtgaagttagctcaagaacttggtctccaacctcgtcaagtagctgtttggtttcagaaccgccgtgcgcggtggaagacaaaacagcttgagaaagattacggtgttctcaaaacgcaatacgattctctccgccataactttgattccctccgccgtgaaaatgaatctcttcttcaagagatcggtaaactaaaagctaagcttaacggagaagaagaaggagatgatgttgatgaagaagagaacaacttggcgacgatggagagtgatgtttccgtcaaggaagaagaagtttcgttgccggagcagatcacagagccgccgtcttctcctccgcagcttctagagcattccgacagtttcaattaccggagtttcaccgacctccgcgaccttcttccgttaaaggccgcggcttcctccgtcgccgccgctggatcgtcggacagtagcgattcgagcgccgtgttgaacgaggaaagtagctctaacgttacggcggctccggcgacggttcccggcggcagtttcttgcagtttgtgaaaatggagcagacggaggatcacgacgactttctgagtggagaagaagcgtgcgggtttttctccgatgaacagccaccgtctctgcactggtattccaccgttgatcagtggaactga

BN45206322 cDNA translates into following amino acid sequences (SEQ ID NO:12):

mmkrlsssdsvgglislcpttstdqpnprrcgrefqsmlegyeeeeeeaiteergqtglaekkrrlninqvkaleknfelenkleperkvklaqelglqprqvavwfqnrrarwktkqlekdygvlktqydslrhnfdslrrenesllqeigklkaklngeeegddvdeeennlatmesdvsvkeeevslpeqiteppssppqllehsdsfnyrsftdlrdllplkaaassvaaagssdssdssavlneesssnvtaapatvpggsflqfvkmeqtedhddflsgeeacgffsdeqppslhwystvdqwn

Come from the GM48923793 cDNA sequence (SEQ ID NO:13) of soybean:

atggcgggtagtggaagtgccttttccaacatcactagctttcttcgcacccaacaaccctcttctcaacctctcgattcttctctcttcctctctgcaccttcctctgctcctttcctcggttcgagatccatgatgagttttgatggagaaggagggaaggggtgtaacggctccttcttccgcgcgtttgacatggacgacaatggggatgagtgcatggacgagtactttcatcaacccgagaagaagcgacgtctctctgcgagccaggttcagtttctagagaagagcttcgaggaggagaacaagcttgaacccgagagaaagaccaaactagccaaagaccttggtttgcagccacggcaagttgctatttggttccagaaccgtagagctcggtggaagaacaaacagctggagaaggattacgagactctgcatgcaagttttgagagtctcaagtccaactatgactgtcttctcaaggagaaagacaagttaaaagctgaggtggcgagcctcactgagaaggtgcttgcaagagggaaacaagaggggcacatgaagcaggctgaaagtgaaagtgaagaaacaaaaggattattgcatttgcaggaacaggaaccaccccagaggcttttactgcaatcagtttcggagggagaaggatccaaagtctcttctgtcgttgggggttgtaaacaggaagatatcagttcagcaaggagtgacattttggattcagatagtccacattacaccgatggagttcactctgcgctgctagagcatggtgattcttcttatgtgtttgagcctgatcaatcagatatgtcacaagatgaagaagataacctcagcaagagtctctacccttcgtacctctttcccaaacttgaagaagatgtggattactccgacccacctgaaagttcttgtaattttggatttcctgaggaagatcatgtcctttggacctgggcttactactaa

GM48923793 cDNA translates into following amino acid sequences (SEQ ID NO:14):

magsgsafsnitsflrtqqpssqpldsslflsapssapflgsrsmmsfdgeggkgcngsffrafdmddngdecmdeyfhqpekkrrlsasqvqfleksfeeenkleperktklakdlglqprqvaiwfqnrrarwknkqlekdyetlhasfeslksnydcllkekdklkaevasltekvlargkqeghmkqaeseseetkgllhlqeqeppqrlllqsvsegegskvssvvggckqedissarsdildsdsphytdgvhsallehgdssyvfepdqsdmsqdeednlskslypsylfpkleedvdysdppesscnfgfpeedhvlwtwayy

Come from the TA55969932 cDNA sequence (SEQ ID NO:15) of wheat:

atggagcccggccggctcatcttcaacacgtcgggctccggcaacggacagatgctcttcatggactgcggcgcgggcggcatcgccggcgcggccggcatgttccatcgaggggtgagaccggtcctcggcggcatggaagaagggcgcggcgtgaagcggcccttcttcacctcgccggatgacatgctggaggaggagtactacgacgagcagctcccggagaagaagcggcgcctcacgccggagcaggtccacctgctggagaggagcttcgaggaggagaacaagctggagccggagaggaagacggagctggcccgcaagctcgggctgcagccacggcaggtggccgtctggttccagaaccgccgcgcccggtggaagacaaagacgctggagcgcgacttcgaccgcctcaaggcgtccttcgacgccctccgcgccgaccacgacgcgctcctccaggacaaccaccggctccggtcacaggtggtaacgttgaccgagaagatgcaagataaggaggcgccggaaggcagcttcggtgcagccgccgacgcctcggagccggagcaggcggcggcggaggcgaaggcttccttggccgacgccgaggagcaggccgcggcagcggaggcgttcgaggtggtgcagcagcagctgcacgtgaaggacgaggagaggctgagcccggggagcggcgggagcgcggtgctggacgcgagggacgcgctgctcgggagcggatgcggcctcgccggcgtggtggacagcagcgtggactcgtactgcttcccggggggcgccggcggcgacgagtaccacgagtgcgtggtgggccccgtggcgggcggcatccagtcggaggaggacgacggcgcgggcagcgacgagggctgcagctactaccccgacgacgccgccgtcttcttcgccgccgcgcaagggcacggccaccatcgcacggacgacgacgatcagcaggacgacggccagatcagctactggatgtggaactag

TA55969932 cDNA translates into following amino acid sequences (SEQ ID NO:16):

mepgrlifntsgsgngqmlfmdcgaggiagaagmfhrgvrpvlggmeegrgvkrpfftspddmleeeyydeqlpekkrrltpeqvhllersfeeenkleperktelarklglqprqvavwfqnrrarwktktlerdfdrlkasfdalradhdallqdnhrlrsqvvtltekmqdkeapegsfgaaadasepeqaaaeakasladaeeqaaaaeafevvqqqlhvkdeerlspgsggsavldardallgsgcglagvvdssvdsycfpggaggdeyhecvvgpvaggiqseeddgagsdegcsyypddaavffaaaqghghhrtddddqqddgqisywmwn

Come from the BN47310186 cDNA sequence (SEQ ID NO:17) of canola oil dish:

atggaccacgacaaaacaggatgccaaagcccacctgaaggtcccaagctatgcatcaacaactgcggtttcttcggaagcgctgccacaatgaacatgtgttccaagtgtcacaaggctatcctgtttcaacaggaacagggggctaggtttgcatctgcagtgtctggtggtacatcatcatccagcaacatcttaaaggaaacctttgctgctaccgcgctggttgatgctgaaaccaaatccgttgagccggtggctgtctctgtacagccatcttctgtccaagttgccgcagaggtagtagctccagaagccgctgcagcaaaactaaaggaaggaccaagccgatgtgctacttgcaataaacgggttggtctgactggattcaaatgtcgctgtggtgacctcttctgcgggacgcaccgttatgcagacatacacaactgctccttcaattaccatgccgctgcgcaagaagctatagctaaagcaaacccggttgtgaaggcagagaagcttgacaaaatctga

BN47310186 cDNA translates into following amino acid sequences (SEQ ID NO:18):

mdhdktgcqsppegpklcinncgffgsaatmnmcskchkailfqqeqgarfasavsggtssssnilketfaatalvdaetksvepvavsvqpssvqvaaevvapeaaaaklkegpsrcatcnkrvgltgfkcrcgdlfcgthryadihncsfnyhaaaqeaiakanpvvkaekldki

Come from the BN51359456 cDNA sequence (SEQ ID NO:19) of canola oil dish

atggcggaagagcatcgatgccagacgccggaaggccaccgtctctgtgctaacaactgcggcttcctcggcagctccgccaccatgaatctatgctccaactgctacggcgatctctgccttaagcaacagcaagcttccatgaaatccaccgtcgaatcctctctctccgccgtatctcctccgtcgtcagagatcggctctatgcaatccaccgttgaatcctctctctccgacgtatctcctccatcaccggagaccatttccatctcctctccaatgatccagcctctcgttcgaaacccatcagctgaattggaggtaacggcgacgaagacggtgactccgccgccggagcagcagcagaaacggccgaatcggtgcacgacgtgtaggaaacgggtcgggttgaccgggttcaagtgccggtgcgggacgactttttgcggggctcacaggtacccggaggtccatggatgcaccttcgatttcaaatcggccggtcgcgaagagatcgccaaggcgaacccactcgtcaaagcggcgaagcttcagaagatttga

BN51359456 cDNA translates into following amino acid sequences (SEQ ID NO:20):

maeehrcqtpeghrlcanncgflgssatmnlcsncygdlclkqqqasmkstvesslsavsppsseigsmqstvesslsdvsppspetisisspmiqplvrnpsaelevtatktvtpppeqqqkrpnrcttcrkrvgltgfkcrcgttfcgahrypevhgctfdfksagreeiakanplvkaaklqki

Come from the HV62552639 cDNA sequence (SEQ ID NO:21) of barley:

atggcccaggagagttgtgatctcaacaaggacgaggccgagatcctgaagccatcctcctccacaccttcgcctccttcgccagccacaccaccaccaccaaccgctcaaataccagaaccacaacctccacactcaccaccacaaccaccggcagctcaattcttgtccaggccctgcgaggttgttcccatagagacttccaaaaagaggaaacatgctgatgcggtgtcaatggccattgtggttgagccattgtcgtctgtgctgttcgttaaccgttgcaacgtgtgccgcaagagagttggtttgaccgggttccgttgccggtgtgagaagctcttttgtccgcgccaccggcattcagaaagccacgactgctcatttgattataaaactgtgggtcgggaggagattgcccgggcaaaccctctgatcagggctgccaagatcattaggatatga

HV62552639 cDNA translates into following amino acid sequences (SEQ ID NO:22):

maqescdlnkdeaeilkpssstpsppspatpppptaqipepqpphsppqppaaqflsrpcevvpietskkrkhadavsmaivveplssvlfvnrcnvcrkrvgltgfrcrceklfcprhrhseshdcsfdyktvgreeiaranpliraakiiri

Come from the ZM61995511 cDNA sequence (SEQ ID NO:23) of corn:

atggaacacaaggaggcgggctgccagcagccggagggcccaatcctatgcatcaataactgcggcttcttcggcagtgctgcgacgatgaacatgtgctccaagtgccacaaggagatgataacgaagcaggagcaggcccagctggctgcctcccccatcgatagcattgtcaatggcggtgacggcgggaaaggacctgtaattgctgcatctgtaaatgtggcagttcctcaagttgagcagaagactattgttgtgcagcccatgcttgtagctgaaaccagcgaggctgctgctgtaatccccaaggccaaggaaggcccagaccggtgcgcggcctgcaggaagcgtgttgggctgacgggatttagctgccgatgcgggaacatgtactgttcggtgcaccgctactccgacaaacatgactgtcagttcgactatcggactgcagcaagggacgcgat?tgccaaggccaatcctgtggtgagggcggagaagctcgacaagatctga

ZM61995511 cDNA translates into following amino acid sequences (SEQ ID NO:24):

mehkeagcqqpcgpilcinncgffgsaatmnmcskchkemi?tkqeqaqlaaspidsivnggdggkgpviaasvnvavpqveqktivvqpmlvaetseaaavipkakegpdrcaacrkrvgltgfscrcgnmycsvhrysdkhdcqfdyrtaardaiakanpvvraekldki

Come from the LU61567101 cDNA sequence (SEQ ID NO:25) of Semen Lini:

atggctccttcaccttgcgtccacggctgcacggccaattgcccccgctgccactcttacggacaccccatcttcgggaactcagatctcgccgctggcggcagcgatacgtccacgtcggtgtttggaaaagtaggatccgtcgtgattcagtcgcctgcgaagaatcacgcgttcggccaagcttgtggcccggtttttccctcgagctcctcccctttccgccgcatcaagttcggccccaaagatggcgaggggaaaggaccgctgaagccgatcgagaagcagccgtcgaagaagcgtccgttctgcttctctcccgacgagacgattgacgcgacggttcctccgtccaccaaaccgttcggttcgttccgttccgtctgtgtcacggacgccgacgaggccaggttgaaggcgaaccgcgagttcttcgctccggtatcccgcaaacgtggcttcgatccgactgacatgaccttcggtaacgccgccgccgctgcggctaatgcgagggaggaagcgaagaagtggtgcggcagttgcaagaagcgcgtggggctgttagggttcaagtgcaggtgtacgaagttcttctgtgggaagcatcggtatcctgaggagcatggttgtacgttcgatcatgtggcgttcgggaggcggattatcgagaaacagaatcctgttctcgagaccgacaagctggtggacagaatctga

LU61567101 cDNA translates into following amino acid sequences (SEQ ID NO:26):

mapspcvhgctancprchsyghpifgnsdlaaggsdtstsvfgkvgsvviqspaknhafgqacgpvfpsssspfrrikfgpkdgegkgplkpiekqpskkrpfcfspdetidatvppstkpfgsfrsvcvtdadearlkanreffapvsrkrgfdptdmtfgnaaaaaanareeakkwcgsckkrvgllgfkcrctkffcgkhrypeehgctfdhvafgrriiekqnpvletdklvdri

Come from the LU61893412 cDNA sequence (SEQ ID NO:27) of Semen Lini:

atggaccatgacgaggcaggctgccaggctccttccgatcatcctattctgtgcgttaacaattgcggcttcttcggaagtgctgccaccatgaacatgtgctcaaagtgccacaaggatacgatgctaaaccaagagcaatccaagcttgctgcttcatcggcagcaagtatcctcaacggatcgtcgatgagcctcggaagggaactcgttattgctgctaagaccaattcggtagaacccaagaccatctccgtccaaccatcttctgcttcaagtgctgaagagagtatcgaaatgaagctgccaaaagaagggcccagtaggtgcaacacttgcaacaaacgtgtcggtttgaccggattcaaatgtcggtgcgagaacatgttctgcgcaaaccatcgctactcggacaagcacaattgcccctttgattaccgcactgctggccgtgaagctatctcaaaggccaatcctttggtgaaggcggagaagctcgacaaaatctga

LU61893412 cDNA translates into following amino acid sequences (SEQ ID NO:28)

mdhdeagcqapsdhpilcvnncgffgsaatmnmcskchkdtmlnqeqsklaassaasilngssmslgrelviaaktnsvepktisvqpssassaeesiemklpkegpsrcntcnkrvgltgfkcrcenmfcanhrysdkhncpfdyrtagreaiskanplvkaekldki

Come from the OS39781852 cDNA sequence (SEQ ID NO:29) of paddy rice:

atggcgcagcgcgacaagaaggatcaggagccgacggagctcagggcgccggagatcacgctgtgcgccaacagctgcggattcccgggcaacccggccacgcagaacctctgccagaactgcttcttggcggccacggcgtccacctcgtcgccgtcttctttgtcgtcaccggtgctcgacaagcagccgccgaggccggcggcgccgctggttgagcctcaggctcctctcccaccgcctgtggaggagatggcctccgcgctcgcgacggcgccggcgccggtcgccaagacgtcggcggtgaaccggtgctccaggtgccggaagcgtgtcggcctcaccgggttccggtgccggtgcggccacctgttctgcggcgagcaccggtactccgaccgccacggctgcagctacgactacaagtcggcggcgagggacgccatcgccagggacaacccggtggtgcgcgcggccaagatcgttaggttctga

OS39781852 cDNA translates into following amino acid sequences (SEQ ID NO:30):

maqrdkkdqeptelrapeitlcanscgfpgnpatqnlcqncflaatastsspsslsspvldkqpprpaaplvepqaplpppveemasalatapapvaktsavnrcsrcrkrvgltgfrcrcghlfcgehrysdrhgcsydyksaardaiardnpvvraakivrf

Come from the OS34701560 cDNA sequence (SEQ ID NO:31) of paddy rice:

atggccgaagaacaccgatgccaagctcccgaaggtcacagactctgctccaacaactgcggtttctttggtagccccgccaccatgaatctctgttccaaatgctacagagacatccgtttgaaggaagaagaacaagccaaaaccaaatccacaatcgaaaccgctctttcaggatcttcctccgccaccgtcaccgcaaccgccgtcgttgcctcctccgtggaatccccttcggcgccggttgaatccctccctcaaccaccggtgctgatttcgccggatatagccgcaccggttcaggcgaaccggtgcggcgcgtgtaggaagcgcgtggggttgacagggttcaagtgcaggtgcggaacaacgttttgtgggagccacaggtaccccgagaaacacgcgtgtggcttcgatttcaaggcggtggggagagaggagatagcacgggcgaatcccgtgatcaaaggcgagaagctacggaggatttaa

OS34701560 cDNA translates into following amino acid sequences (SEQ ID NO:32):

maeehrcqapeghrlcsnncgffgspatmnlcskcyrdirlkeeeqaktkstietalsgsssatvtatavvassvespsapveslpqppvlispdiaapvqanrcgacrkrvgltgfkcrcgttfcgshrypekhacgfdfkavgreeiaranpvikgeklrri

Come from the OS36821256 cDNA sequence (SEQ ID NO:33) of paddy rice:

atggcgcagagggagaagaaggtggaggagccgacggagctgagggcgccggagatgacgctctgcgccaacagctgcgggttcccgggcaacccggcgaccaacaacctctgccagaactgcttcttggctgcctcggcgtcttcttcttcttcttccgccgctgcctcgccgtcgacgacgtcgttgccggtgtttccggtggtggagaagccgaggcaggccgtacagtcgtcggcggcggcggcggtggcgctggtggttgagcggccgacggcggggccggtggagtcgtcgtcgaaggcgtcgaggtcgtcgtcggtcaaccgatgccacagctgccggaggcgggtgggcctgaccgggttccggtgccgctgcggcgagctctactgcggcgcgcaccggtactccgaccgccacgactgcagcttcgactacaagtcggcggcgagggacgccatcgccagggagaaccccgtcgtccgcgccgccaagatcgttaggttctaa

OS36821256 cDNA translates into following amino acid sequences (SEQ ID NO:34):

maqrekkveeptelrapemtlcanscgfpgnpatnnlcqncflaasassssssaaaspsttslpvfpvvekprqavqssaaaavalvverptagpvessskasrsssvnrchscrrrvgltgfrcrcgelycgahrysdrhdcsfdyksaardaiarenpvvraakivrf

Come from the GM51659494 cDNA sequence (SEQ ID NO:35) of soybean:

atggctcagaaaaccgagaaagaagaaaccgacttcaaagttccggaaacgattacgctttgcgtcaacaactgcggcgtcaccggaaaccctgccacgaataacatgtgccagaagtgcttcactgcctctaccgccaccacttccggcgccggaggtgccggaatagcttctccggcgaccagatccggcgtctccgcgcgtcctcagaagagatcttttcctgaagagccctcgccggtggcggatcctccttcttcggaccagacgacgccgtcggaggcgaagcgcgtggtcaaccgctgctccggatgccggcggaaggtcggactcaccggattccggtgccggtgcggcgagctcttctgcgccgagcaccggtactccgaccgccacgactgcagctatgactacaaagccgccggaagagaagccatcgcgagggagaatccggtgatcagagctgcgaagatcgtcaaagtctga

GM51659494 cDNA translates into following amino acid sequences (SEQ ID NO:36):

maqktekeetdfkvpetitlcvnncgvtgnpatnnmcqkcftastattsgaggagiaspatrsgvsarpqkrsfpeepspvadppssdqttpseakrvvnrcsgcrrkvgl?tgfrcrcgelfcaehrysdrhdcsydykaagreaiarenpviraakivkv

Come from the GM49780101 cDNA sequence (SEQ ID NO:37) of soybean:

atggagcctcatgatgagactggatgccaggctcctgaacgccccattctttgcattaataattgtggcttctttggaagagcagctaccatgaacatgtgttccaagtgttacaaggacatgctgttgaagcaggagcaggacaaatttgcagcatcatccgttgaaaacattgtgaatggcagttccaatggcaatggaaagcaggctgtggctactggtgctgttgctgtacaagttgaagctgtggaggtcaagattgtctgtgctcagagttctgtggattcgtcctccggtgatagtttggagatgaaagccaagactggtcccagtagatgtgctacatgccggaaacgtgttggtttaactggtttcagctgcaaatgtggcaacctcttctgtgcaatgcatcgctattctgataaacatgattgcccttttgattataggactgttggtcaggatgccatagctaaagccaaccccataattaaggcagataagctcgacaaaatctag

GM49780101 cDNA translates into following amino acid sequences (SEQ ID NO:38)

mephdetgcqaperpilcinncgffgraatmnmcskcykdmllkqeqdkfaassvenivngssngngkqavatgavavqveavevkivcaqssvdsssgdslemkaktgpsrcatcrkrvgltgfsckcgnlfcamhrysdkhdcpfdyrtvgqdaiakanpiikadkldki

Come from the GM59637305 cDNA sequence (SEQ ID NO:39) of soybean:

atggaccatgacaagactgggtgccaagctcctcctgaaggtcctatattgtgcatcaacaactgtgggttttttggaagtgcagctaccatgaacatgtgttctaaatgccacaaagacatattgctgaaacaggagcaggccaagcttgcagcatcatccattgggaatattatgaatgggtcatcaagcagcactgaaaaggaacctgttgttgctgctgctgctaatattgatatcccagttattccagtagagcctaaaactgtctctgtgcaacctttatttggttcaggtccagaggggagtgttgaggcaaagccgaaggatggaccaaaacgttgcagcagctgcaacaagcgagttggtttgacagggtttaattgtcgatgtggtgacctttttttgtgctgtacatcgctactcgacaagcataattgcccatttgattaccgcactgccgctcaagatgctatagctaaagcaaacccagttgtcaaggctgaaaagcttgataagatctaa

GM59637305 cDNA translates into following amino acid sequences (SEQ ID NO:40):

mdhdktgcqappegpilcinncgffgsaatmnmcskchkdillkqeqaklaassignimngsssstekepvvaaaanidipvipvepktvsvqplfgsgpegsveakpkdgpkrcsscnkrvgltgfncrcgdlflcctslldkhncpfdyrtaaqdaiakanpvvkaekldki

Come from the TA55974113 cDNA sequence (SEQ ID NO:41) of wheat:

atggcgcagcgggatcacaagcaggaggagcccacggagctgcgggcgccggagatcacgctctgcgccaacagctgcggcttcccgggcaacccggccacgcagaacctctgccagaactgcttcttggccggcccggcgtccacgtcgccgtcttcctcctcctcctcctcctcttctctgccgggcgtgtccgcgccgacccccgtcatcgacaggccgaggccggcgccgttggaggcggagctggcacgccccgccgtcgaccttgctccggcgacggaggcgaagccggcgaggacgtcggtgaaccggtgctccagctgccggaagcgcgtggggctgacggggttccggtgccggtgcggcgacatgttctgcggcgagcaccggtactcggaccggcacgggtgcagctacgactacaaggccgccgccagggacgccatcgccagggacaaccccgtcgtgcgcgccgccaagatcgtcaggttctga

TA55974113 cDNA translates into following amino acid sequences (SEQ ID NO:42)

maqrdhkqeeptelrapeitlcanscgfpgnpatqnlcqncflagpastspssssssssslpgvsaptpvidrprpapleaelarpavdlapateakpartsvnrcsscrkrvgltgfrcrcgdmfcgehrysdrhgcsydykaaardaiardnpvvraakivrf

EST65 aminoacid sequence (SEQ ID NO:43)

mvaesvlvcrssvvgaglqsfvgegakresagpgrsvflgaqvqkmgagmsarsdvrpaavpkasgdvseqtdyktfsdeewkkrlsqqqfyvarkkgterpftgeywntktagtylcvccktplfssktkfdsgtgwpsyydtigdnvkshmdwsipfmprtevvcavcdahlghvfddgprptgkrycinsaaidlkaekqeern

EST12 aminoacid sequence (SEQ ID NO:44)

mvvpslpafggqnamlrrnidnntdtlisllqgscsprvsmqqvprsseslenmmgacgqklpyfssfdgpsveeqedvdegidefahhvekkrrlsleqvrslernfevenkleperkmqlakelglrprqvavwfqnrrarwktkqlehdyetlkkaydrlkadfeavtldtnalkaevsrlkgisnddvkpaefvqgkcdttshpaspaqsersdivssrnrttptihvdpvapeeagahltmssdsnssevmdadsprtshtsasrstlstsvvqpdeglgvaqyphfspenfvgpnmpeicadqslasqvkleeihsfnpdqtflllpnwwdwa

EST307 aminoacid sequence (SEQ ID NO:45)

matervsqettsqapegpvmcknlcgffgsqatmglcskcyretvmqakmtalaeqatqaaqatsataaavqppapvhetkltcevertmivphqsssyqqdlvtpaaaapqavkssiaapsrpepnrcgscrkrvgltgfkcrcgnlycalhrysdkhtctydykaagqeaiakanplvvaekvvkf

Sequence table

＜110〉BASF. Plant Science GmbH

 

＜120〉have the stress tolerance of increase and the transgenic plant of output

 

<130>PF57972

 

<160>45

 

<170>PatentIn?version?3.4

 

<210>1

<211>615

<212>DNA

＜213〉colea

 

<220>

<221>CDS

<222>(1)..(615)

＜223〉methionine sulfoxide reductase family protein (BN51364980)

 

<400>1

atg?gct?tct?tct?agt?tgt?ttc?acc?att?cag?tca?cgt?ttc?gtc?tca?gcg?????48

Met?Ala?Ser?Ser?Ser?Cys?Phe?Thr?Ile?Gln?Ser?Arg?Phe?Val?Ser?Ala

1???????????????5???????????????????10??????????????????15

aga?aca?aag?ctc?gat?tca?atc?tcc?aaa?ccg?agt?ctc?tcc?gga?ttc?gct?????96

Arg?Thr?Lys?Leu?Asp?Ser?Ile?Ser?Lys?Pro?Ser?Leu?Ser?Gly?Phe?Ala

20??????????????????25??????????????????30

tgt?cgt?tct?ctt?aca?aaa?ccc?aga?aac?ttg?aat?ctc?tct?gtt?ctt?ctt????144

Cys?Arg?Ser?Leu?Thr?Lys?Pro?Arg?Asn?Leu?Asn?Leu?Ser?Val?Leu?Leu

35??????????????????40??????????????????45

cgg?tgt?tcc?atg?ggt?tcc?ttt?aac?tct?tct?cag?aaa?tca?gac?aac?gtc????192

Arg?Cys?Ser?Met?G1y?Ser?Phe?Asn?Ser?Ser?Gln?Lys?Ser?Asp?Ash?Val

50??????????????????55??????????????????60

caa?gaa?gct?gca?aag?agt?gac?ttt?gct?tca?ata?agt?gaa?ggt?gag?tgg????240

Gln?Glu?Ala?Ala?Lys?Ser?Asp?Phe?Ala?Ser?Ile?Ser?Glu?Gly?Glu?Trp

65??????????????????70??????????????????75??????????????????80

aag?aaa?cgg?cta?aca?cca?gaa?cag?tat?tac?atc?acc?aga?cag?aag?gga????288

Lys?Lys?Arg?Leu?Thr?Pro?Glu?Gln?Tyr?Tyr?Ile?Thr?Arg?Gln?Lys?Gly

85??????????????????90??????????????????95

aca?gag?aga?gct?ttc?act?ggt?gag?tat?tgg?aat?aca?aag?acc?cca?gga????336

Thr?Glu?Arg?Ala?Phe?Thr?Gly?Glu?Tyr?Trp?Asn?Thr?Lys?Thr?Pro?Gly

100?????????????????105?????????????????110

gta?tac?aaa?tgt?atc?tgt?tgc?gac?acg?cca?ctg?ttt?gac?tca?tca?aca????384

Val?Tyr?Lys?Cys?Ile?Cys?Cys?Asp?Thr?Pro?Leu?Phe?Asp?Ser?Ser?Thr

115?????????????????120?????????????????125

aag?ctt?gat?agt?gga?acc?ggg?tgg?cca?tcg?tat?tac?caa?cct?att?gga????432

Lys?Leu?Asp?Ser?Gly?Thr?Gly?Trp?Pro?Ser?Tyr?Tyr?Gln?Pro?Ile?Gly

130?????????????????135?????????????????140

aac?aat?gtg?aag?tca?aag?ctg?gac?ctc?tct?atc?atc?ttc?atg?cct?aga????480

Asn?Asn?Val?Lys?Ser?Lys?Leu?Asp?Leu?Ser?Ile?Ile?Phe?Met?Pro?Arg

145?????????????????150?????????????????155?????????????????160

caa?gaa?gtt?atc?tgt?gct?gtt?tgt?aac?gcc?cat?ctt?ggt?cat?gtc?ttc????528

Gln?Glu?Val?Ile?Cys?Ala?Val?Cys?Asn?Ala?His?Leu?Gly?His?Val?Phe

165?????????????????170?????????????????175

gat?gac?ggt?cca?cga?cca?acc?gga?aaa?cga?tat?tgc?ctc?aac?agt?gct????576

Asp?Asp?Gly?Pro?Arg?Pro?Thr?Gly?Lys?Arg?Tyr?Cys?Leu?Asn?Ser?Ala

180?????????????????185?????????????????190

gct?ctg?aaa?ctt?gag?tca?ttg?gag?aga?aca?aga?gaa?tga????????????????615

Ala?Leu?Lys?Leu?Glu?Ser?Leu?Glu?Arg?Thr?Arg?Glu

195?????????????????200

 

<210>2

<211>204

<212>PRT

＜213〉colea

 

<400>2

 

Met?Ala?Ser?Ser?Ser?Cys?Phe?Thr?Ile?Gln?Ser?Arg?Phe?Val?Ser?Ala

1???????????????5???????????????????10??????????????????15

Arg?Thr?Lys?Leu?Asp?Ser?Ile?Ser?Lys?Pro?Ser?Leu?Ser?Gly?Phe?Ala

20??????????????????25??????????????????30

Cys?Arg?Ser?Leu?Thr?Lys?Pro?Arg?Asn?Leu?Asn?Leu?Ser?Val?Leu?Leu

35??????????????????40??????????????????45

Arg?Cys?Ser?Met?Gly?Ser?Phe?Asn?Ser?Ser?Gln?Lys?Ser?Asp?Asn?Val

50??????????????????55??????????????????60

Gln?Glu?Ala?Ala?Lys?Ser?Asp?Phe?Ala?Ser?Ile?Ser?Glu?Gly?Glu?Trp

65??????????????????70??????????????????75??????????????????80

Lys?Lys?Arg?Leu?Thr?Pro?Glu?Gln?Tyr?Tyr?Ile?Thr?Arg?Gln?Lys?Gly

85??????????????????90??????????????????95

Thr?Glu?Arg?Ala?Phe?Thr?Gly?Glu?Tyr?Trp?Asn?Thr?Lys?Thr?Pro?Gly

100?????????????????105?????????????????110

Val?Tyr?Lys?Cys?Ile?Cys?Cys?Asp?Thr?Pro?Leu?Phe?Asp?Ser?Ser?Thr

115?????????????????120?????????????????125

Lys?Leu?Asp?Ser?Gly?Thr?Gly?Trp?Pro?Ser?Tyr?Tyr?Gln?Pro?Ile?Gly

130?????????????????135?????????????????140

Asn?Asn?Val?Lys?Ser?Lys?Leu?Asp?Leu?Ser?Ile?Ile?Phe?Met?Pro?Arg

145?????????????????150?????????????????155?????????????????160

Gln?Glu?Val?Ile?Cys?Ala?Val?Cys?Asn?Ala?His?Leu?Gly?His?Val?Phe

165?????????????????170?????????????????175

Asp?Asp?Gly?Pro?Arg?Pro?Thr?Gly?Lys?Arg?Tyr?Cys?Leu?Asn?Ser?Ala

180?????????????????185?????????????????190

Ala?Leu?Lys?Leu?Glu?Ser?Leu?Glu?Arg?Thr?Arg?Glu

195?????????????????200

 

<210>3

<211>615

<212>DNA

＜213〉rice

 

<220>

<221>CDS

<222>(1)..(615)

＜223〉methionine sulfoxide reductase family protein (OS34096188)

<400>3

atg?ggc?ttc?aat?att?ctg?aga?acc?act?tcc?atc?tcc?act?cct?atc?tct?????48

Met?Gly?Phe?Asn?Ile?Leu?Arg?Thr?Thr?Ser?Ile?Ser?Thr?Pro?Ile?Ser

1???????????????5???????????????????10??????????????????15

tcc?tcc?aaa?tcc?aaa?ccc?att?ttc?tca?act?ctt?ctt?cgt?tct?tct?cct?????96

Ser?Ser?Lys?Ser?Lys?Pro?Ile?Phe?Ser?Thr?Leu?Leu?Arg?Ser?Ser?Pro

20??????????????????25??????????????????30

tcc?acc?att?ttc?ccc?cca?aag?tcc?gtt?act?ccc?acc?act?ctt?ttc?gtt????144

Ser?Thr?Ile?Phe?Pro?Pro?Lys?Ser?Val?Thr?Pro?Thr?Thr?Leu?Phe?Val

35??????????????????40??????????????????45

tct?gcc?acc?ccc?ttc?ttc?act?ctc?cat?ccc?aag?ctt?ggt?ttt?cgt?ggt????192

Ser?Ala?Thr?Pro?Phe?Phe?Thr?Leu?His?Pro?Lys?Leu?Gly?Phe?Arg?Gly

50??????????????????55??????????????????60

ggg?att?gtg?gcc?atg?gcc?gca?cct?ggc?tct?ctc?cgc?aaa?tcc?gag?gaa????240

Gly?Ile?Val?Ala?Met?Ala?Ala?Pro?Gly?Ser?Leu?Arg?Lys?Ser?Glu?Glu

65??????????????????70??????????????????75??????????????????80

gag?tgg?cgc?gca?att?ctc?tcc?cct?gaa?cag?ttt?cgg?atc?ctc?agg?caa????288

Glu?Trp?Arg?Ala?Ile?Leu?Ser?Pro?Glu?Gln?Phe?Arg?Ile?Leu?Arg?Gln

85??????????????????90??????????????????95

aag?ggc?acc?gag?ttc?cct?gga?aca?gga?gag?tat?gac?aag?ttc?tat?gaa????336

Lys?Gly?Thr?Glu?Phe?Pro?Gly?Thr?Gly?Glu?Tyr?Asp?Lys?Phe?Tyr?Glu

100?????????????????105?????????????????110

gag?gga?gtt?tac?aac?tgt?gct?ggt?tgt?ggg?act?cca?ctc?tac?agg?tcc????384

Glu?Gly?Val?Tyr?Asn?Cys?Ala?Gly?Cys?Gly?Thr?Pro?Leu?Tyr?Arg?Ser

115?????????????????120?????????????????125

ata?aca?aaa?ttc?aat?tct?ggt?tgt?ggc?tgg?cca?gcc?ttc?tat?gag?ggg????432

Ile?Thr?Lys?Phe?Asn?Ser?Gly?Cys?Gly?Trp?Pro?Ala?Phe?Tyr?Glu?Gly

130?????????????????135?????????????????140

att?ccc?gga?gcc?ata?aat?cgc?aat?ccg?gat?cct?gat?ggg?atg?agg?aca????480

Ile?Pro?Gly?Ala?Ile?Asn?Arg?Asn?Pro?Asp?Pro?Asp?Gly?Met?Arg?Thr

145?????????????????150?????????????????155?????????????????160

gaa?ata?acg?tgt?gct?gct?tgt?ggg?gga?cat?cta?ggt?cac?gtc?ttt?aaa????528

Glu?Ile?Thr?Cys?Ala?Ala?Cys?Gly?Gly?His?Leu?Gly?His?Val?Phe?Lys

165?????????????????170?????????????????175

gga?gaa?gga?ttt?cca?aca?ccc?act?aac?gaa?cgc?cat?tgt?gtc?aat?agc????576

Gly?Glu?Gly?Phe?Pro?Thr?Pro?Thr?Asn?Glu?Arg?His?Cys?Val?Asn?Ser

180?????????????????185?????????????????190

att?tcg?ctg?aaa?ttt?gcg?cca?gcc?aat?tct?tat?tct?taa????????????????615

Ile?Ser?Leu?Lys?Phe?Ala?Pro?Ala?Asn?Ser?Tyr?Ser

195?????????????????200

 

<210>4

<211>204

<212>PRT

＜213〉rice

 

<400>4

 

Met?Gly?Phe?Asn?Ile?Leu?Arg?Thr?Thr?Ser?Ile?Ser?Thr?Pro?Ile?Ser

1???????????????5???????????????????10??????????????????15

Ser?Ser?Lys?Ser?Lys?Pro?Ile?Phe?Ser?Thr?Leu?Leu?Arg?Ser?Ser?Pro

20??????????????????25??????????????????30

Ser?Thr?Ile?Phe?Pro?Pro?Lys?Ser?Val?Thr?Pro?Thr?Thr?Leu?Phe?Val

35??????????????????40??????????????????45

Ser?Ala?Thr?Pro?Phe?Phe?Thr?Leu?His?Pro?Lys?Leu?Gly?Phe?Arg?Gly

50??????????????????55??????????????????60

Gly?Ile?Val?Ala?Met?Ala?Ala?Pro?Gly?Ser?Leu?Arg?Lys?Ser?Glu?Glu

65??????????????????70??????????????????75??????????????????80

Glu?Trp?Arg?Ala?Ile?Leu?Ser?Pro?Glu?Gln?Phe?Arg?Ile?Leu?Arg?Gln

85??????????????????90??????????????????95

Lys?Gly?Thr?Glu?Phe?Pro?Gly?Thr?Gly?Glu?Tyr?Asp?Lys?Phe?Tyr?Glu

100?????????????????105?????????????????110

Glu?Gly?Val?Tyr?Asn?Cys?Ala?Gly?Cys?Gly?Thr?Pro?Leu?Tyr?Arg?Ser

115?????????????????120?????????????????125

Ile?Thr?Lys?Phe?Asn?Ser?Gly?Cys?Gly?Trp?Pro?Ala?Phe?Tyr?Glu?Gly

130?????????????????135?????????????????140

Ile?Pro?Gly?Ala?Ile?Asn?Arg?Asn?Pro?Asp?Pro?Asp?Gly?Met?Arg?Thr

145?????????????????150?????????????????155?????????????????160

Glu?Ile?Thr?Cys?Ala?Ala?Cys?Gly?Gly?His?Leu?Gly?His?Val?Phe?Lys

165?????????????????170?????????????????175

Gly?Glu?Gly?Phe?Pro?Thr?Pro?Thr?Asn?Glu?Arg?His?Cys?Val?Asn?Ser

180?????????????????185?????????????????190

Ile?Ser?Leu?Lys?Phe?Ala?Pro?Ala?Asn?Ser?Tyr?Ser

195?????????????????200

 

<210>5

<211>645

<212>DNA

＜213〉rice

 

<220>

<221>CDS

<222>(1)..(645)

＜223〉methionine sulfoxide reductase family protein (OS32583643)

 

<400>5

atg?gcc?atg?cgg?caa?tac?gcg?gct?gct?acc?gct?gcc?tcc?tcc?agt?ttc?????48

Met?Ala?Met?Arg?Gln?Tyr?Ala?Ala?Ala?Thr?Ala?Ala?Ser?Ser?Ser?Phe

1???????????????5???????????????????10??????????????????15

aga?gca?cgt?cca?cgg?gcg?cgc?ccc?tcc?tgc?crc?cca?gcc?gcc?gcc?ctg?????96

Arg?Ala?Arg?Pro?Arg?Ala?Arg?Pro?Ser?Cys?Leu?Pro?Ala?Ala?Ala?Leu

20??????????????????25??????????????????30

ccc?ttg?gcg?cct?tgc?tgt?ggt?gtg?gcg?tgg?agc?cgt?gct?agc?tac?agg????144

Pro?Leu?Ala?Pro?Cys?Cys?Gly?Val?Ala?Trp?Ser?Arg?Ala?Ser?Tyr?Arg

35??????????????????40??????????????????45

cga?gcc?tcc?gtt?cgt?gcc?atg?ggt?gcc?gct?tca?tcg?tct?tcg?tcg?tcg????192

Arg?Ala?Ser?Val?Arg?Ala?Met?Gly?Ala?Ala?Ser?Ser?Ser?Ser?Ser?Ser

50??????????????????55??????????????????60

tcg?tcg?tcg?tct?ccg?tcg?ccg?cag?ggt?caa?gcc?caa?gcc?caa?gcc?caa????240

Ser?Ser?Ser?Ser?Pro?Ser?Pro?Gln?Gly?Gln?Ala?Gln?Ala?Gln?Ala?Gln

65??????????????????70??????????????????75??????????????????80

ggt?aaa?ccg?aac?tac?agt?aca?tct?ctg?act?gat?gag?gag?tgg?agg?aag????288

Gly?Lys?Pro?Asn?Tyr?Ser?Thr?Ser?Leu?Thr?Asp?Glu?Glu?Trp?Arg?Lys

85??????????????????90??????????????????95

cgc?ctg?aca?aaa?gat?cag?tat?tac?att?act?cgg?cag?aag?ggc?aca?gaa????336

Arg?Leu?Thr?Lys?Asp?Gln?Tyr?Tyr?Ile?Thr?Arg?Gln?Lys?Gly?Thr?Glu

100?????????????????105?????????????????110

aga?gca?ttt?act?ggg?gaa?tac?tgg?aac?acc?aaa?acc?ccg?ggc?atc?tac????384

Arg?Ala?Phe?Thr?Gly?Glu?Tyr?Trp?Asn?Thr?Lys?Thr?Pro?Gly?Ile?Tyr

115?????????????????120?????????????????125

cat?tgt?gtc?tgc?tgt?gac?acc?cct?ctt?ttt?gag?tca?tcg?acc?aaa?ttt????432

His?Cys?Val?Cys?Cys?Asp?Thr?Pro?Leu?Phe?Glu?Ser?Ser?Thr?Lys?Phe

130?????????????????135?????????????????140

gat?agt?ggt?act?ggg?tgg?ccg?tca?tat?tat?caa?ccc?att?gga?gat?aat????480

Asp?Ser?Gly?Thr?Gly?Trp?Pro?Ser?Tyr?Tyr?Gln?Pro?Ile?Gly?Asp?Asn

145?????????????????150?????????????????155?????????????????160

gta?aag?tgc?aag?ctt?gat?atg?tcc?atc?ata?ttc?atg?cct?cgg?act?gag????528

Val?Lys?Cys?Lys?Leu?Asp?Met?Ser?Ile?Ile?Phe?Met?Pro?Arg?Thr?Glu

165?????????????????170?????????????????175

gtg?ctg?tgt?gct?gtc?tgt?gac?gct?cat?ctg?ggg?cac?gtg?ttt?gat?gat????576

Val?Leu?Cys?Ala?Val?Cys?Asp?Ala?His?Leu?Gly?His?Val?Phe?Asp?Asp

180?????????????????185?????????????????190

ggg?cca?cga?cca?aca?ggg?aaa?aga?tac?tgt?atc?aat?agc?gca?tct?ctc????624

Gly?Pro?Arg?Pro?Thr?Gly?Lys?Arg?Tyr?Cys?Ile?Asn?Ser?Ala?Ser?Leu

195?????????????????200?????????????????205

aag?ctg?aag?aag?acc?cag?tag????????????????????????????????????????645

Lys?Leu?Lys?Lys?Thr?Gln

210

 

<210>6

<211>214

<212>PRT

＜213〉rice

 

<400>6

Met?Ala?Met?Arg?Gln?Tyr?Ala?Ala?Ala?Thr?Ala?Ala?Ser?Ser?Ser?Phe

1???????????????5???????????????????10??????????????????15

Arg?Ala?Arg?Pro?Arg?Ala?Arg?Pro?Ser?Cys?Leu?Pro?Ala?Ala?Ala?Leu

20??????????????????25??????????????????30

Pro?Leu?Ala?Pro?Cys?Cys?Gly?Val?Ala?Trp?Ser?Arg?Ala?Ser?Tyr?Arg

35??????????????????40??????????????????45

Arg?Ala?Ser?Val?Arg?Ala?Met?Gly?Ala?Ala?Ser?Ser?Ser?Ser?Ser?Ser

50??????????????????55??????????????????60

Ser?Ser?Ser?Ser?Pro?Ser?Pro?Gln?Gly?Gln?Ala?Gln?Ala?Gln?Ala?Gln

65??????????????????70??????????????????75??????????????????80

Gly?Lys?Pro?Asn?Tyr?Ser?Thr?Ser?Leu?Thr?Asp?Glu?Glu?Trp?Arg?Lys

85??????????????????90??????????????????95

Arg?Leu?Thr?Lys?Asp?Gln?Tyr?Tyr?Ile?Thr?Arg?Gln?Lys?Gly?Thr?Glu

100?????????????????105?????????????????110

Arg?Ala?Phe?Thr?Gly?Glu?Tyr?Trp?Asn?Thr?Lys?Thr?Pro?Gly?Ile?Tyr

115?????????????????120?????????????????125

His?Cys?Val?Cys?Cys?Asp?Thr?Pro?Leu?Phe?Glu?Ser?Ser?Thr?Lys?Phe

130?????????????????135?????????????????140

Asp?Ser?Gly?Thr?Gly?Trp?Pro?Ser?Tyr?Tyr?Gln?Pro?Ile?Gly?Asp?Asn

145?????????????????150?????????????????155?????????????????160

Val?Lys?Cys?Lys?Leu?Asp?Met?Ser?Ile?Ile?Phe?Met?Pro?Arg?Thr?Glu

165?????????????????170?????????????????175

Val?Leu?Cys?Ala?Val?Cys?Asp?Ala?His?Leu?Gly?His?Val?Phe?Asp?Asp

180?????????????????185?????????????????190

Gly?Pro?Arg?Pro?Thr?Gly?Lys?Arg?Tyr?Cys?Ile?Asn?Ser?Ala?Ser?Leu

195?????????????????200?????????????????205

Lys?Leu?Lys?Lys?Thr?Gln

210

<210>7

<211>609

<212>DNA

＜213〉soybean

 

<220>

<221>CDS

<222>(1)..(609)

＜223〉methionine sulfoxide reductase family protein (GM53626178)

 

<400>7

atg?gga?ttg?agt?att?ctg?aga?agc?act?tcc?att?tcc?act?cct?atc?tct?????48

Met?Gly?Leu?Ser?Ile?Leu?Arg?Ser?Thr?Ser?Ile?Ser?Thr?Pro?Ile?Ser

1???????????????5???????????????????10??????????????????15

tcc?tcc?aaa?tcc?aaa?ccc?att?ttc?tca?act?ctt?gtt?cgt?tca?tct?ttc?????96

Ser?Ser?Lys?Ser?Lys?Pro?Ile?Phe?Ser?Thr?Leu?Val?Arg?Ser?Ser?Phe

20??????????????????25??????????????????30

gcc?tcc?att?tcc?ccc?aca?aag?tgt?gtt?act?ccc?acc?act?ctt?ttc?gtt????144

Ala?Ser?Ile?Ser?Pro?Thr?Lys?Cys?Val?Thr?Pro?Thr?Thr?Leu?Phe?Val

35??????????????????40??????????????????45

tct?gcc?acc?ccc?ttc?ttc?acc?gcc?tca?ccc?aag?cgc?ggc?ttt?cgt?ggt????192

Ser?Ala?Thr?Pro?Phe?Phe?Thr?Ala?Ser?Pro?Lys?Arg?Gly?Phe?Arg?Gly

50??????????????????55??????????????????60

ggg?att?gtg?gcc?atg?gcc?gcc?gct?ggc?tcg?ctc?cgc?aaa?tca?gag?gaa????240

Gly?Ile?Val?Ala?Met?Ala?Ala?Ala?Gly?Ser?Leu?Arg?Lys?Ser?Glu?Glu

65??????????????????70??????????????????75??????????????????80

gag?tgg?cgc?gca?gtt?ctc?tcc?cct?gaa?cag?ttt?cgt?att?ctc?agg?caa????288

Glu?Trp?Arg?Ala?Val?Leu?Ser?Pro?Glu?Gln?Phe?Arg?Ile?Leu?Arg?Gln

85??????????????????90??????????????????95

aag?ggc?acc?gag?ttc?cct?gga?aca?gga?gag?tat?gac?aag?ttc?ttt?gat????336

Lys?Gly?Thr?Glu?Phe?Pro?Gly?Thr?Gly?Glu?Tyr?Asp?Lys?Phe?Phe?Asp

100?????????????????105?????????????????110

gag?gga?gtt?tac?aac?tgt?gct?ggt?tgt?ggg?aca?cct?ctc?tac?agg?tcc????384

Glu?Gly?Val?Tyr?Asn?Cys?Ala?Gly?Cys?Gly?Thr?Pro?Leu?Tyr?Arg?Ser

115?????????????????120?????????????????125

tta?aca?aaa?ttc?aat?tct?ggt?tgt?ggc?tgg?cca?gcc?ttc?tat?gag?ggg????432

Leu?Thr?Lys?Phe?Asn?Ser?Gly?Cys?Gly?Trp?Pro?Ala?Phe?Tyr?Glu?Gly

130?????????????????135?????????????????140

att?cct?gga?gcc?ata?aat?cgc?aat?ccg?gac?cct?gat?ggg?atg?agg?aca????480

Ile?Pro?Gly?Ala?Ile?Asn?Arg?Asn?Pro?Asp?Pro?Asp?Gly?Met?Arg?Thr

145?????????????????150?????????????????155?????????????????160

gaa?ata?acg?tgt?gct?gct?tgt?ggg?gga?cat?cta?ggt?cac?gtc?ttt?aaa????528

Glu?Ile?Thr?Cys?Ala?Ala?Cys?Gly?Gly?His?Leu?Gly?His?Val?Phe?Lys

165?????????????????170?????????????????175

gga?gaa?gga?ttt?cca?acg?ccc?act?aac?gaa?cgc?cat?tgt?gtc?aat?agc????576

Gly?Glu?Gly?Phe?Pro?Thr?Pro?Thr?Asn?Glu?Arg?His?Cys?Val?Asn?Ser

180?????????????????185?????????????????190

att?tca?ctg?aaa?ttt?gcg?cca?gcc?aat?tct?taa????????????????????????609

Ile?Ser?Leu?Lys?Phe?Ala?Pro?Ala?Asn?Ser

195?????????????????200

 

<210>8

<211>202

<212>PRT

＜213〉soybean

 

<400>8

 

Met?Gly?Leu?Ser?Ile?Leu?Arg?Ser?Thr?Ser?Ile?Ser?Thr?Pro?Ile?Ser

1???????????????5???????????????????10??????????????????15

Ser?Ser?Lys?Ser?Lys?Pro?Ile?Phe?Ser?Thr?Leu?Val?Arg?Ser?Ser?Phe

20??????????????????25??????????????????30

Ala?Ser?Ile?Ser?Pro?Thr?Lys?Cys?Val?Thr?Pro?Thr?Thr?Leu?Phe?Val

35??????????????????40??????????????????45

Ser?Ala?Thr?Pro?Phe?Phe?Thr?Ala?Ser?Pro?Lys?Arg?Gly?Phe?Arg?Gly

50??????????????????55??????????????????60

Gly?Ile?Val?Ala?Met?Ala?Ala?Ala?Gly?Ser?Leu?Arg?Lys?Ser?Glu?Glu

65??????????????????70??????????????????75??????????????????80

Glu?Trp?Arg?Ala?Val?Leu?Ser?Pro?Glu?Gln?Phe?Arg?Ile?Leu?Arg?Gln

85??????????????????90??????????????????95

Lys?Gly?Thr?Glu?Phe?Pro?Gly?Thr?Gly?Glu?Tyr?Asp?Lys?Phe?Phe?Asp

100?????????????????105?????????????????110

Glu?Gly?Val?Tyr?Asn?Cys?Ala?Gly?Cys?Gly?Thr?Pro?Leu?Tyr?Arg?Ser

115?????????????????120?????????????????125

Leu?Thr?Lys?Phe?Asn?Ser?Gly?Cys?Gly?Trp?Pro?Ala?Phe?Tyr?Glu?Gly

130?????????????????135?????????????????140

Ile?Pro?Gly?Ala?Ile?Asn?Arg?Asn?Pro?Asp?Pro?Asp?Gly?Met?Arg?Thr

145?????????????????150?????????????????155?????????????????160

Glu?Ile?Thr?Cys?Ala?Ala?Cys?Gly?Gly?His?Leu?Gly?His?Val?Phe?Lys

165?????????????????170?????????????????175

Gly?Glu?Gly?Phe?Pro?Thr?Pro?Thr?Asn?Glu?Arg?His?Cys?Val?Asn?Ser

180?????????????????185?????????????????190

Ile?Ser?Leu?Lys?Phe?Ala?Pro?Ala?Asn?Ser

195?????????????????200

 

<210>9

<211>621

<212>DNA

＜213〉wheat

 

<220>

<221>CDS

<222>(1)..(621)

＜223〉methionine sulfoxide reductase family protein (TA6540264)

 

<400>9

atg?gcg?tcg?ccc?cac?gcc?cac?ccg?gcc?acg?cgg?ccc?ctc?tca?tcg?ctc?????48

Met?Ala?Ser?Pro?His?Ala?His?Pro?Ala?Thr?Arg?Pro?Leu?Ser?Ser?Leu

1???????????????5???????????????????10??????????????????15

ccg?tcc?ctc?ctc?ctc?gcc?cgc?tcc?tcc?tcc?gcc?gcc?acc?gcc?gcc?gcg?????96

Pro?Ser?Leu?Leu?Leu?Ala?Arg?Ser?Ser?Ser?Ala?Ala?Thr?Ala?Ala?Ala

20??????????????????25??????????????????30

tcg?tcc?gcc?cgc?ccc?gcc?tcc?ctc?tcc?ctc?tcg?tgc?tcg?cgg?tcg?cgg????144

Ser?Ser?Ala?Arg?Pro?Ala?Ser?Leu?Ser?Leu?Ser?Cys?Ser?Arg?Ser?Arg

35??????????????????40??????????????????45

gcg?cgg?gcc?tac?tgc?cca?gcc?gga?cga?cgg?ttg?ccg?ggc?gcc?gtg?gtg????192

Ala?Arg?Ala?Tyr?Cys?Pro?Ala?Gly?Arg?Arg?Leu?Pro?Gly?Ala?Val?Val

50??????????????????55??????????????????60

gct?atg?tcg?tcg?gcg?gcg?ccc?acg?ccg?ggg?ccc?gtg?cag?aag?tcg?gag????240

Ala?Met?Ser?Ser?Ala?Ala?Pro?Thr?Pro?Gly?Pro?Val?Gln?Lys?Ser?Glu

65??????????????????70??????????????????75??????????????????80

gag?gag?tgg?gag?gcc?gtc?ctc?acg?ccg?gag?cag?ttc?cgc?atc?ctc?cgc????288

Glu?Glu?Trp?Glu?Ala?Val?Leu?Thr?Pro?Glu?Gln?Phe?Arg?Ile?Leu?Arg

85??????????????????90??????????????????95

cgc?aag?ggc?acc?gag?tat?cct?gga?aca?ggt?gaa?tat?gac?aag?ttc?ttc????336

Arg?Lys?Gly?Thr?Glu?Tyr?Pro?Gly?Thr?Gly?Glu?Tyr?Asp?Lys?Phe?Phe

100?????????????????105?????????????????110

agt?gag?ggt?att?tac?gga?tgt?gct?ggc?tgt?gga?acc?ccc?ttg?tac?aaa????384

Ser?Glu?Gly?Ile?Tyr?Gly?Cys?Ala?Gly?Cys?Gly?Thr?Pro?Leu?Tyr?Lys

115?????????????????120?????????????????125

tca?tct?acg?aag?ttc?aac?tca?ggg?tgt?ggt?tgg?cca?gca?ttc?tat?gaa????432

Ser?Ser?Thr?Lys?Phe?Asn?Ser?Gly?Cys?Gly?Trp?Pro?Ala?Phe?Tyr?Glu

130?????????????????135?????????????????140

gga?ttt?cct?gga?gcc?ata?aaa?cgg?acg?gcg?gat?cct?gat?ggg?agg?cga????480

Gly?Phe?Pro?Gly?Ala?Ile?Lys?Arg?Thr?Ala?Asp?Pro?Asp?Gly?Arg?Arg

145?????????????????150?????????????????155?????????????????160

att?gag?atc?aca?tgt?gct?gct?tgt?gaa?gga?cat?ctg?ggg?cat?gtg?ttc????528

Ile?Glu?Ile?Thr?Cys?Ala?Ala?Cys?Glu?Gly?His?Leu?Gly?His?Val?Phe

165?????????????????170?????????????????175

aaa?ggg?gag?ggg?ttc?aac?acg?ccg?act?gat?gag?cga?cac?tgc?gtc?aac????576

Lys?Gly?Glu?Gly?Phe?Asn?Thr?Pro?Thr?Asp?Glu?Arg?His?Cys?Val?Asn

180?????????????????185?????????????????190

agt?atc?tca?ctc?aag?ttc?gtt?ccg?gcc?tct?gaa?gag?gct?agt?tga????????621

Ser?Ile?Ser?Leu?Lys?Phe?Val?Pro?Ala?Ser?Glu?Glu?Ala?Ser

195?????????????????200?????????????????205

 

<210>10

<211>206

<212>PRT

＜213〉wheat

 

<400>10

 

Met?Ala?Ser?Pro?His?Ala?His?Pro?Ala?Thr?Arg?Pro?Leu?Ser?Ser?Leu

1???????????????5???????????????????10??????????????????15

Pro?Ser?Leu?Leu?Leu?Ala?Arg?Ser?Ser?Ser?Ala?Ala?Thr?Ala?Ala?Ala

20??????????????????25??????????????????30

Ser?Ser?Ala?Arg?Pro?Ala?Ser?Leu?Ser?Leu?Ser?Cys?Ser?Arg?Ser?Arg

35??????????????????40??????????????????45

Ala?Arg?Ala?Tyr?Cys?Pro?Ala?Gly?Arg?Arg?Leu?Pro?Gly?Ala?Val?Val

50??????????????????55??????????????????60

Ala?Met?Ser?Ser?Ala?Ala?Pro?Thr?Pro?Gly?Pro?Val?Gln?Lys?Ser?Glu

65??????????????????70??????????????????75??????????????????80

Glu?Glu?Trp?Glu?Ala?Val?Leu?Thr?Pro?Glu?Gln?Phe?Arg?Ile?Leu?Arg

85??????????????????90??????????????????95

Arg?Lys?Gly?Thr?Glu?Tyr?Pro?Gly?Thr?Gly?Glu?Tyr?Asp?Lys?Phe?Phe

100?????????????????105?????????????????110

Ser?Glu?Gly?Ile?Tyr?Gly?Cys?Ala?Gly?Cys?Gly?Thr?Pro?Leu?Tyr?Lys

115?????????????????120?????????????????125

Ser?Ser?Thr?Lys?Phe?Asn?Ser?Gly?Cys?Gly?Trp?Pro?Ala?Phe?Tyr?Glu

130?????????????????135?????????????????140

Gly?Phe?Pro?Gly?Ala?Ile?Lys?Arg?Thr?Ala?Asp?Pro?Asp?Gly?Arg?Arg

145?????????????????150?????????????????155?????????????????160

Ile?Glu?Ile?Thr?Cys?Ala?Ala?Cys?Glu?Gly?His?Leu?Gly?His?Val?Phe

165?????????????????170?????????????????175

Lys?Gly?Glu?Gly?Phe?Asn?Thr?Pro?Thr?Asp?Glu?Arg?His?Cys?Val?Asn

180?????????????????185?????????????????190

Ser?Ile?Ser?Leu?Lys?Phe?Val?Pro?Ala?Ser?Glu?Glu?Ala?Ser

195?????????????????200?????????????????205

<210>11

<211>933

<212>DNA

＜213〉colea

 

<220>

<221>CDS

<222>(1)..(933)

＜223〉homeodomain-leucine zipper protein matter (BN45206322)

 

<400>11

atg?atg?aag?aga?tta?agc?agt?tca?gat?tca?gtg?ggt?ggt?ctc?atc?tct?????48

Met?Met?Lys?Arg?Leu?Ser?Ser?Ser?Asp?Ser?Val?Gly?Gly?Leu?Ile?Ser

1???????????????5???????????????????10??????????????????15

tta?tgt?ccc?act?act?tcc?aca?gat?cag?ccg?aat?cca?aga?aga?tgc?ggg?????96

Leu?Cys?Pro?Thr?Thr?Ser?Thr?Asp?Gln?Pro?Asn?Pro?Arg?Arg?Cys?Gly

20??????????????????25??????????????????30

aga?gaa?ttt?cag?tcg?atg?ctc?gaa?ggt?tac?gag?gag?gaa?gaa?gaa?gaa????144

Arg?Glu?Phe?Gln?Ser?Met?Leu?Glu?Gly?Tyr?Glu?Glu?Glu?Glu?Glu?Glu

35??????????????????40??????????????????45

gcc?ata?acc?gag?gaa?aga?gga?caa?acc?ggt?tta?gcc?gag?aag?aag?aga????192

Ala?Ile?Thr?Glu?Glu?Arg?Gly?Gln?Thr?Gly?Leu?Ala?Glu?Lys?Lys?Arg

50??????????????????55??????????????????60

cgg?tta?aac?att?aac?caa?gtt?aaa?gcc?ttg?gag?aaa?aat?ttc?gag?tta????240

Arg?Leu?Asn?Ile?Asn?Gln?Val?Lys?Ala?Leu?Glu?Lys?Asn?Phe?Glu?Leu

65??????????????????70??????????????????75??????????????????80

gag?aac?aag?ctt?gag?cct?gag?agg?aaa?gtg?aag?tta?gct?caa?gaa?ctt????288

Glu?Asn?Lys?Leu?Glu?Pro?Glu?Arg?Lys?Val?Lys?Leu?Ala?Gln?Glu?Leu

85??????????????????90??????????????????95

ggt?ctc?caa?cct?cgt?caa?gta?gct?gtt?tgg?ttt?cag?aac?cgc?cgt?gcg????336

Gly?Leu?Gln?Pro?Arg?Gln?Val?Ala?Val?Trp?Phe?Gln?Asn?Arg?Arg?Ala

100?????????????????105?????????????????110

cgg?tgg?aag?aca?aaa?cag?ctt?gag?aaa?gat?tac?ggt?gtt?ctc?aaa?acg????384

Arg?Trp?Lys?Thr?Lys?Gln?Leu?Glu?Lys?Asp?Tyr?Gly?Val?Leu?Lys?Thr

115?????????????????120?????????????????125

caa?tac?gat?tct?ctc?cgc?cat?aac?ttt?gat?tcc?ctc?cgc?cgt?gaa?aat????432

Gln?Tyr?Asp?Ser?Leu?Arg?His?Asn?Phe?Asp?Ser?Leu?Arg?Arg?Glu?Asn

130?????????????????135?????????????????140

gaa?tct?ctt?ctt?caa?gag?atc?ggt?aaa?cta?aaa?gct?aag?ctt?aac?gga????480

Glu?Ser?Leu?Leu?Gln?Glu?Ile?Gly?Lys?Leu?Lys?Ala?Lys?Leu?Asn?Gly

145?????????????????150?????????????????155?????????????????160

gaa?gaa?gaa?gga?gat?gat?gtt?gat?gaa?gaa?gag?aac?aac?ttg?gcg?acg????528

Glu?Glu?Glu?Gly?Asp?Asp?Val?Asp?Glu?Glu?Glu?Asn?Asn?Leu?Ala?Thr

165?????????????????170?????????????????175

atg?gag?agt?gat?gtt?tcc?gtc?aag?gaa?gaa?gaa?gtt?tcg?ttg?ccg?gag????576

Met?Glu?Ser?Asp?Val?Ser?Val?Lys?Glu?Glu?Glu?Val?Ser?Leu?Pro?Glu

180?????????????????185?????????????????190

cag?atc?aca?gag?ccg?ccg?tct?tct?cct?ccg?cag?ctt?cta?gag?cat?tcc????624

Gln?Ile?Thr?Glu?Pro?Pro?Ser?Ser?Pro?Pro?Gln?Leu?Leu?Glu?His?Ser

195?????????????????200?????????????????205

gac?agt?ttc?aat?tac?cgg?agt?ttc?acc?gac?ctc?cgc?gac?ctt?ctt?ccg????672

Asp?Ser?Phe?Asn?Tyr?Arg?Ser?Phe?Thr?Asp?Leu?Arg?Asp?Leu?Leu?Pro

210?????????????????215?????????????????220

tta?aag?gcc?gcg?gct?tcc?tcc?gtc?gcc?gcc?gct?gga?tcg?tcg?gac?agt????720

Leu?Lys?Ala?Ala?Ala?Ser?Ser?Val?Ala?Ala?Ala?Gly?Ser?Ser?Asp?Ser

225?????????????????230?????????????????235?????????????????240

agc?gat?tcg?agc?gcc?gtg?ttg?aac?gag?gaa?agt?agc?tct?aac?gtt?acg????768

Ser?Asp?Ser?Ser?Ala?Val?Leu?Asn?Glu?Glu?Ser?Ser?Ser?Asn?Val?Thr

245?????????????????250?????????????????255

gcg?gct?ccg?gcg?acg?gtt?ccc?ggc?ggc?agt?ttc?ttg?cag?ttt?gtg?aaa????816

Ala?Ala?Pro?Ala?Thr?Val?Pro?Gly?Gly?Ser?Phe?Leu?Gln?Phe?Val?Lys

260?????????????????265?????????????????270

atg?gag?cag?acg?gag?gat?cac?gac?gac?ttt?ctg?agt?gga?gaa?gaa?gcg????864

Met?Glu?Gln?Thr?Glu?Asp?His?Asp?Asp?Phe?Leu?Ser?Gly?Glu?Glu?Ala

275?????????????????280?????????????????285

tgc?ggg?ttt?ttc?tcc?gat?gaa?cag?cca?ccg?tct?ctg?cac?tgg?tat?tcc????912

Cys?Gly?Phe?Phe?Ser?Asp?Glu?Gln?Pro?Pro?Ser?Leu?His?Trp?Tyr?Ser

290?????????????????295?????????????????300

acc?gtt?gat?cag?tgg?aac?tga????????????????????????????????????????933

Thr?Val?Asp?Gln?Trp?Asn

305?????????????????310

 

<210>12

<211>310

<212>PRT

＜213〉colea

 

<400>12

 

Met?Met?Lys?Arg?Leu?Ser?Ser?Ser?Asp?Ser?Val?Gly?Gly?Leu?Ile?Ser

1???????????????5???????????????????10??????????????????15

Leu?Cys?Pro?Thr?Thr?Ser?Thr?Asp?Gln?Pro?Asn?Pro?Arg?Arg?Cys?Gly

20??????????????????25??????????????????30

Arg?Glu?Phe?Gln?Ser?Met?Leu?Glu?Gly?Tyr?Glu?Glu?Glu?Glu?Glu?Glu

35??????????????????40??????????????????45

Ala?Ile?Thr?Glu?Glu?Arg?Gly?Gln?Thr?Gly?Leu?Ala?Glu?Lys?Lys?Arg

50??????????????????55??????????????????60

Arg?Leu?Asn?Ile?Asn?Gln?Val?Lys?Ala?Leu?Glu?Lys?Asn?Phe?Glu?Leu

65??????????????????70??????????????????75??????????????????80

Glu?Asn?Lys?Leu?Glu?Pro?Glu?Arg?Lys?Val?Lys?Leu?Ala?Gln?Glu?Leu

85??????????????????90??????????????????95

Gly?Leu?Gln?Pro?Arg?Gln?Val?Ala?Val?Trp?Phe?Gln?Asn?Arg?Arg?Ala

100?????????????????105?????????????????110

Arg?Trp?Lys?Thr?Lys?Gln?Leu?Glu?Lys?Asp?Tyr?Gly?Val?Leu?Lys?Thr

115?????????????????120?????????????????125

Gln?Tyr?Asp?Ser?Leu?Arg?His?Asn?Phe?Asp?Ser?Leu?Arg?Arg?Glu?Asn

130?????????????????135?????????????????140

Glu?Ser?Leu?Leu?Gln?Glu?Ile?Gly?Lys?Leu?Lys?Ala?Lys?Leu?Asn?Gly

145?????????????????150?????????????????155?????????????????160

Glu?Glu?Glu?Gly?Asp?Asp?Val?Asp?Glu?Glu?Glu?Asn?Asn?Leu?Ala?Thr

165?????????????????170?????????????????175

Met?Glu?Ser?Asp?Val?Ser?Val?Lys?Glu?Glu?Glu?Val?Ser?Leu?Pro?Glu

180?????????????????185?????????????????190

Gln?Ile?Thr?Glu?Pro?Pro?Ser?Ser?Pro?Pro?Gln?Leu?Leu?Glu?His?Ser

195?????????????????200?????????????????205

Asp?Ser?Phe?Asn?Tyr?Arg?Ser?Phe?Thr?Asp?Leu?Arg?Asp?Leu?Leu?Pro

210?????????????????215?????????????????220

Leu?Lys?Ala?Ala?Ala?Ser?Ser?Val?Ala?Ala?Ala?Gly?Ser?Ser?Asp?Ser

225?????????????????230?????????????????235?????????????????240

Ser?Asp?Ser?Ser?Ala?Val?Leu?Asn?Glu?Glu?Ser?Ser?Ser?Asn?Val?Thr

245?????????????????250?????????????????255

Ala?Ala?Pro?Ala?Thr?Val?Pro?Gly?Gly?Ser?Phe?Leu?Gln?Phe?Val?Lys

260?????????????????265?????????????????270

Met?Glu?Gln?Thr?Glu?Asp?His?Asp?Asp?Phe?Leu?Ser?Gly?Glu?Glu?Ala

275?????????????????280?????????????????285

Cys?Gly?Phe?Phe?Ser?Asp?Glu?Gln?Pro?Pro?Ser?Leu?His?Trp?Tyr?Ser

290?????????????????295?????????????????300

Thr?Val?Asp?Gln?Trp?Asn

305?????????????????310

 

<210>13

<211>996

<212>DNA

＜213〉soybean

 

<220>

<221>CDS

<222>(1)..(996)

＜223〉homeodomain-leucine zipper protein matter (GM48923793)

 

<400>13

atg?gcg?ggt?agt?gga?agt?gcc?ttt?tcc?aac?atc?act?agc?ttt?ctt?cgc????48

Met?Ala?Gly?Ser?Gly?Ser?Ala?Phe?Ser?Asn?Ile?Thr?Ser?Phe?Leu?Arg

1???????????????5???????????????????10??????????????????15

acc?caa?caa?ccc?tct?tct?caa?cct?ctc?gat?tct?tct?ctc?ttc?ctc?tct?????96

Thr?Gln?Gln?Pro?Ser?Ser?Gln?Pro?Leu?Asp?Ser?Ser?Leu?Phe?Leu?Ser

20??????????????????25??????????????????30

gca?cct?tcc?tct?gct?cct?ttc?ctc?ggt?tcg?aga?tcc?atg?atg?agt?ttt????144

Ala?Pro?Ser?Ser?Ala?Pro?Phe?Leu?Gly?Ser?Arg?Ser?Met?Met?Ser?Phe

35??????????????????40??????????????????45

gat?gga?gaa?gga?ggg?aag?ggg?tgt?aac?ggc?tcc?ttc?ttc?cgc?gcg?ttt????192

Asp?Gly?Glu?Gly?Gly?Lys?Gly?Cys?Asn?Gly?Ser?Phe?Phe?Arg?Ala?Phe

50??????????????????55??????????????????60

gac?atg?gac?gac?aat?ggg?gat?gag?tgc?atg?gac?gag?tac?ttt?cat?caa????240

Asp?Met?Asp?Asp?Asn?Gly?Asp?Glu?Cys?Met?Asp?Glu?Tyr?Phe?His?Gln

65??????????????????70??????????????????75??????????????????80

ccc?gag?aag?aag?cga?cgt?ctc?tct?gcg?agc?cag?gtt?cag?ttt?cta?gag????288

Pro?Glu?Lys?Lys?Arg?Arg?Leu?Ser?Ala?Ser?Gln?Val?Gln?Phe?Leu?Glu

85??????????????????90??????????????????95

aag?agc?ttc?gag?gag?gag?aac?aag?ctt?gaa?ccc?gag?aga?aag?acc?aaa????336

Lys?Ser?Phe?Glu?Glu?Glu?Asn?Lys?Leu?Glu?Pro?Glu?Arg?Lys?Thr?Lys

100?????????????????105?????????????????110

cta?gcc?aaa?gac?ctt?ggt?ttg?cag?cca?cgg?caa?gtt?gct?att?tgg?ttc????384

Leu?Ala?Lys?Asp?Leu?Gly?Leu?Gln?Pro?Arg?Gln?Val?Ala?Ile?Trp?Phe

115?????????????????120?????????????????125

cag?aac?cgt?aga?gct?cgg?tgg?aag?aac?aaa?cag?ctg?gag?aag?gat?tac????432

Gln?Asn?Arg?Arg?Ala?Arg?Trp?Lys?Asn?Lys?Gln?Leu?Glu?Lys?Asp?Tyr

130?????????????????135?????????????????140

gag?act?ctg?cat?gca?agt?ttt?gag?agt?ctc?aag?tcc?aac?tat?gac?tgt????480

Glu?Thr?Leu?His?Ala?Ser?Phe?Glu?Ser?Leu?Lys?Ser?Asn?Tyr?Asp?Cys

145?????????????????150?????????????????155?????????????????160

ctt?ctc?aag?gag?aaa?gac?aag?tta?aaa?gct?gag?gtg?gcg?agc?ctc?act????528

Leu?Leu?Lys?Glu?Lys?Asp?Lys?Leu?Lys?Ala?Glu?Val?Ala?Ser?Leu?Thr

165?????????????????170?????????????????175

gag?aag?gtg?ctt?gca?aga?ggg?aaa?caa?gag?ggg?cac?atg?aag?cag?gct????576

Glu?Lys?Val?Leu?Ala?Arg?Gly?Lys?Gln?Glu?Gly?His?Met?Lys?Gln?Ala

180?????????????????185?????????????????190

gaa?agt?gaa?agt?gaa?gaa?aca?aaa?gga?tta?ttg?cat?ttg?cag?gaa?cag????624

Glu?Ser?Glu?Ser?Glu?Glu?Thr?Lys?Gly?Leu?Leu?His?Leu?Gln?Glu?Gln

195?????????????????200?????????????????205

gaa?cca?ccc?cag?agg?ctt?tta?ctg?caa?tca?gtt?tcg?gag?gga?gaa?gga????672

Glu?Pro?Pro?Gln?Arg?Leu?Leu?Leu?Gln?Ser?Val?Ser?Glu?Gly?Glu?Gly

210?????????????????215?????????????????220

tcc?aaa?gtc?tct?tct?gtc?gtt?ggg?ggt?tgt?aaa?cag?gaa?gat?atc?agt????720

Ser?Lys?Val?Ser?Ser?Val?Val?Gly?Gly?Cys?Lys?Gln?Glu?Asp?Ile?Ser

225?????????????????230?????????????????235?????????????????240

tca?gca?agg?agt?gac?att?ttg?gat?tca?gat?agt?cca?cat?tac?acc?gat????768

Ser?Ala?Arg?Ser?Asp?Ile?Leu?Asp?Ser?Asp?Ser?Pro?His?Tyr?Thr?Asp

245?????????????????250?????????????????255

gga?gtt?cac?tct?gcg?ctg?cta?gag?cat?ggt?gat?tct?tct?tat?gtg?ttt????816

Gly?Val?His?Ser?Ala?Leu?Leu?Glu?His?Gly?Asp?Ser?Ser?Tyr?Val?Phe

260?????????????????265?????????????????270

gag?cct?gat?caa?tca?gat?atg?tca?caa?gat?gaa?gaa?gat?aac?ctc?agc????864

Glu?Pro?Asp?Gln?Ser?Asp?Met?Ser?Gln?Asp?Glu?Glu?Asp?Asn?Leu?Ser

275?????????????????280?????????????????285

aag?agt?ctc?tac?cct?tcg?tac?ctc?ttt?ccc?aaa?ctt?gaa?gaa?gat?gtg????912

Lys?Ser?Leu?Tyr?Pro?Ser?Tyr?Leu?Phe?Pro?Lys?Leu?Glu?Glu?Asp?Val

290?????????????????295?????????????????300

gat?tac?tcc?gac?cca?cct?gaa?agt?tct?tgt?aat?ttt?gga?ttt?cct?gag????960

Asp?Tyr?Ser?Asp?Pro?Pro?Glu?Ser?Ser?Cys?Asn?Phe?Gly?Phe?Pro?Glu

305?????????????????310?????????????????315?????????????????320

gaa?gat?cat?gtc?ctt?tgg?acc?tgg?gct?tac?tac?taa????????????????????996

Glu?Asp?His?Val?Leu?Trp?Thr?Trp?Ala?Tyr?Tyr

325?????????????????330

 

<210>14

<211>331

<212>PRT

＜213〉soybean

 

<400>14

Met?Ala?Gly?Ser?Gly?Ser?Ala?Phe?Ser?Asn?Ile?Thr?Ser?Phe?Leu?Arg

1???????????????5???????????????????10??????????????????15

Thr?Gln?Gln?Pro?Ser?Ser?Gln?Pro?Leu?Asp?Ser?Ser?Leu?Phe?Leu?Ser

20??????????????????25??????????????????30

Ala?Pro?Ser?Ser?Ala?Pro?Phe?Leu?Gly?Ser?Arg?Ser?Met?Met?Ser?Phe

35??????????????????40??????????????????45

Asp?Gly?Glu?Gly?Gly?Lys?Gly?Cys?Asn?Gly?Ser?Phe?Phe?Arg?Ala?Phe

50??????????????????55??????????????????60

Asp?Met?Asp?Asp?Asn?Gly?Asp?Glu?Cys?Met?Asp?Glu?Tyr?Phe?His?Gln

65??????????????????70??????????????????75??????????????????80

Pro?Glu?Lys?Lys?Arg?Arg?Leu?Ser?Ala?Ser?Gln?Val?Gln?Phe?Leu?Glu

85??????????????????90??????????????????95

Lys?Ser?Phe?Glu?Glu?Glu?Asn?Lys?Leu?Glu?Pro?Glu?Arg?Lys?Thr?Lys

100?????????????????105?????????????????110

Leu?Ala?Lys?Asp?Leu?Gly?Leu?Gln?Pro?Arg?Gln?Val?Ala?Ile?Trp?Phe

115?????????????????120?????????????????125

Gln?Asn?Arg?Arg?Ala?Arg?Trp?Lys?Asn?Lys?Gln?Leu?Glu?Lys?Asp?Tyr

130?????????????????135?????????????????140

Glu?Thr?Leu?His?Ala?Ser?Phe?Glu?Ser?Leu?Lys?Ser?Asn?Tyr?Asp?Cys

145?????????????????150?????????????????155?????????????????160

Leu?Leu?Lys?Glu?Lys?Asp?Lys?Leu?Lys?Ala?Glu?Val?Ala?Ser?Leu?Thr

165?????????????????170?????????????????175

Glu?Lys?Val?Leu?Ala?Arg?Gly?Lys?Gln?Glu?Gly?His?Met?Lys?Gln?Ala

180?????????????????185?????????????????190

Glu?Ser?Glu?Ser?Glu?Glu?Thr?Lys?Gly?Leu?Leu?His?Leu?Gln?Glu?Gln

195?????????????????200?????????????????205

Glu?Pro?Pro?Gln?Arg?Leu?Leu?Leu?Gln?Ser?Val?Ser?Glu?Gly?Glu?Gly

210?????????????????215?????????????????220

Ser?Lys?Val?Ser?Ser?Val?Val?Gly?Gly?Cys?Lys?Gln?Glu?Asp?Ile?Ser

225?????????????????230?????????????????235?????????????????240

Ser?Ala?Arg?Ser?Asp?Ile?Leu?Asp?Ser?Asp?Ser?Pro?His?Tyr?Thr?Asp

245?????????????????250?????????????????255

Gly?Val?His?Ser?Ala?Leu?Leu?Glu?His?Gly?Asp?Ser?Ser?Tyr?Val?Phe

260?????????????????265?????????????????270

Glu?Pro?Asp?Gln?Ser?Asp?Met?Ser?Gln?Asp?Glu?Glu?Asp?Asn?Leu?Ser

275?????????????????280?????????????????285

Lys?Ser?Leu?Tyr?Pro?Ser?Tyr?Leu?Phe?Pro?Lys?Leu?Glu?Glu?Asp?Val

290?????????????????295?????????????????300

Asp?Tyr?Ser?Asp?Pro?Pro?Glu?Ser?Ser?Cys?Asn?Phe?Gly?Phe?Pro?Glu

305?????????????????310?????????????????315?????????????????320

Glu?Asp?His?Val?Leu?Trp?Thr?Trp?Ala?Tyr?Tyr

325?????????????????330

 

<210>15

<211>1023

<212>DNA

＜213〉wheat

 

<220>

<221>CDS

<222>(1)..(1023)

＜223〉homeodomain-leucine zipper protein matter (TA55969932)

 

<400>15

atg?gag?ccc?ggc?cgg?ctc?atc?ttc?aac?acg?tcg?ggc?tcc?ggc?aac?gga?????48

Met?Glu?Pro?Gly?Arg?Leu?Ile?Phe?Asn?Thr?Ser?Gly?Ser?Gly?Asn?Gly

1???????????????5???????????????????10??????????????????15

cag?atg?ctc?ttc?atg?gac?tgc?ggc?gcg?ggc?ggc?atc?gcc?ggc?gcg?gcc?????96

Gln?Met?Leu?Phe?Met?Asp?Cys?Gly?Ala?Gly?Gly?Ile?Ala?Gly?Ala?Ala

20??????????????????25??????????????????30

ggc?atg?ttc?cat?cga?ggg?gtg?aga?ccg?gtc?ctc?ggc?ggc?atg?gaa?gaa????144

Gly?Met?Phe?His?Arg?Gly?Val?Arg?Pro?Val?Leu?Gly?Gly?Met?Glu?Glu

35??????????????????40??????????????????45

ggg?cgc?ggc?gtg?aag?cgg?ccc?ttc?ttc?acc?tcg?ccg?gat?gac?atg?ctg????192

Gly?Arg?Gly?Val?Lys?Arg?Pro?Phe?Phe?Thr?Ser?Pro?Asp?Asp?Met?Leu

50??????????????????55??????????????????60

gag?gag?gag?tac?tac?gac?gag?cag?ctc?ccg?gag?aag?aag?cgg?cgc?ctc????240

Glu?Glu?Glu?Tyr?Tyr?Asp?Glu?Gln?Leu?Pro?Glu?Lys?Lys?Arg?Arg?Leu

65??????????????????70??????????????????75??????????????????80

acg?ccg?gag?cag?gtc?cac?ctg?ctg?gag?agg?agc?ttc?gag?gag?gag?aac????288

Thr?Pro?Glu?Gln?Val?His?Leu?Leu?Glu?Arg?Ser?Phe?Glu?Glu?Glu?Asn

85??????????????????90??????????????????95

aag?ctg?gag?ccg?gag?agg?aag?acg?gag?ctg?gcc?cgc?aag?ctc?ggg?ctg????336

Lys?Leu?Glu?Pro?Glu?Arg?Lys?Thr?Glu?Leu?Ala?Arg?Lys?Leu?Gly?Leu

100?????????????????105?????????????????110

cag?cca?cgg?cag?gtg?gcc?gtc?tgg?ttc?cag?aac?cgc?cgc?gcc?cgg?tgg????384

Gln?Pro?Arg?Gln?Val?Ala?Val?Trp?Phe?Gln?Asn?Arg?Arg?Ala?Arg?Trp

115?????????????????120?????????????????125

aag?aca?aag?acg?ctg?gag?cgc?gac?ttc?gac?cgc?ctc?aag?gcg?tcc?ttc????432

Lys?Thr?Lys?Thr?Leu?Glu?Arg?Asp?Phe?Asp?Arg?Leu?Lys?Ala?Ser?Phe

130?????????????????135?????????????????140

gac?gcc?ctc?cgc?gcc?gac?cac?gac?gcg?ctc?ctc?cag?gac?aac?cac?cgg????480

Asp?Ala?Leu?Arg?Ala?Asp?His?Asp?Ala?Leu?Leu?Gln?Asp?Asn?His?Arg

145?????????????????150?????????????????155?????????????????160

ctc?cgg?tca?cag?gtg?gta?acg?ttg?acc?gag?aag?atg?caa?gat?aag?gag????528

Leu?Arg?Ser?Gln?Val?Val?Thr?Leu?Thr?Glu?Lys?Met?Gln?Asp?Lys?Glu

165?????????????????170?????????????????175

gcg?ccg?gaa?ggc?agc?ttc?ggt?gca?gcc?gcc?gac?gcc?tcg?gag?ccg?gag????576

Ala?Pro?Glu?Gly?Ser?Phe?Gly?Ala?Ala?Ala?Asp?Ala?Ser?Glu?Pro?Glu

180?????????????????185?????????????????190

cag?gcg?gcg?gcg?gag?gcg?aag?gct?tcc?ttg?gcc?gac?gcc?gag?gag?cag????624

Gln?Ala?Ala?Ala?Glu?Ala?Lys?Ala?Ser?Leu?Ala?Asp?Ala?Glu?Glu?Gln

195?????????????????200?????????????????205

gcc?gcg?gca?gcg?gag?gcg?ttc?gag?gtg?gtg?cag?cag?cag?ctg?cac?gtg????672

Ala?Ala?Ala?Ala?Glu?Ala?Phe?Glu?Val?Val?Gln?Gln?Gln?Leu?His?Val

210?????????????????215?????????????????220

aag?gac?gag?gag?agg?ctg?agc?ccg?ggg?agc?ggc?ggg?agc?gcg?gtg?ctg????720

Lys?Asp?Glu?Glu?Arg?Leu?Ser?Pro?Gly?Ser?Gly?Gly?Ser?Ala?Val?Leu

225?????????????????230?????????????????235?????????????????240

gac?gcg?agg?gac?gcg?ctg?ctc?ggg?agc?gga?tgc?ggc?ctc?gcc?ggc?gtg????768

Asp?Ala?Arg?Asp?Ala?Leu?Leu?Gly?Ser?Gly?Cys?Gly?Leu?Ala?Gly?Val

245?????????????????250?????????????????255

gtg?gac?agc?agc?gtg?gac?tcg?tac?tgc?ttc?ccg?ggg?ggc?gcc?ggc?ggc????816

Val?Asp?Ser?Ser?Val?Asp?Ser?Tyr?Cys?Phe?Pro?Gly?Gly?Ala?Gly?Gly

260?????????????????265?????????????????270

gac?gag?tac?cac?gag?tgc?gtg?gtg?ggc?ccc?gtg?gcg?ggc?ggc?atc?cag????864

Asp?Glu?Tyr?His?Glu?Cys?Val?Val?Gly?Pro?Val?Ala?Gly?Gly?Ile?Gln

275?????????????????280?????????????????285

tcg?gag?gag?gac?gac?ggc?gcg?ggc?agc?gac?gag?ggc?tgc?agc?tac?tac????912

Ser?Glu?Glu?Asp?Asp?Gly?Ala?Gly?Ser?Asp?Glu?Gly?Cys?Ser?Tyr?Tyr

290?????????????????295?????????????????300

ccc?gac?gac?gcc?gcc?gtc?ttc?ttc?gcc?gcc?gcg?caa?ggg?cac?ggc?cac????960

Pro?Asp?Asp?Ala?Ala?Val?Phe?Phe?Ala?Ala?Ala?Gln?Gly?Hi?s?Gly?His

305?????????????????310?????????????????315?????????????????320

cat?cgc?acg?gac?gac?gac?gat?cag?cag?gac?gac?ggc?cag?atc?agc?tac???1008

His?Arg?Thr?Asp?Asp?Asp?Asp?Gln?Gln?Asp?Asp?Gly?Gln?Ile?Ser?Tyr

325?????????????????330?????????????????335

tgg?atg?tgg?aac?tag???????????????????????????????????????????????1023

Trp?Met?Trp?Asn

340

 

<210>16

<211>340

<212>PRT

＜213〉wheat

 

<400>16

 

Met?Glu?Pro?Gly?Arg?Leu?Ile?Phe?Asn?Thr?Ser?Gly?Ser?Gly?Asn?Gly

1???????????????5???????????????????10??????????????????15

Gln?Met?Leu?Phe?Met?Asp?Cys?Gly?Ala?Gly?Gly?Ile?Ala?Gly?Ala?Ala

20??????????????????25??????????????????30

Gly?Met?Phe?His?Arg?Gly?Val?Arg?Pro?Val?Leu?Gly?Gly?Met?Glu?Glu

35??????????????????40??????????????????45

Gly?Arg?Gly?Val?Lys?Arg?Pro?Phe?Phe?Thr?Ser?Pro?Asp?Asp?Met?Leu

50??????????????????55??????????????????60

Glu?Glu?Glu?Tyr?Tyr?Asp?Glu?Gln?Leu?Pro?Glu?Lys?Lys?Arg?Arg?Leu

65??????????????????70??????????????????75??????????????????80

Thr?Pro?Glu?Gln?Val?His?Leu?Leu?Glu?Arg?Ser?Phe?Glu?Glu?Glu?Asn

85??????????????????90??????????????????95

Lys?Leu?Glu?Pro?Glu?Arg?Lys?Thr?Glu?Leu?Ala?Arg?Lys?Leu?Gly?Leu

100?????????????????105?????????????????110

Gln?Pro?Arg?Gln?Val?Ala?Val?Trp?Phe?Gln?Asn?Arg?Arg?Ala?Arg?Trp

115?????????????????120?????????????????125

Lys?Thr?Lys?Thr?Leu?Glu?Arg?Asp?Phe?Asp?Arg?Leu?Lys?Ala?Ser?Phe

130?????????????????135?????????????????140

Asp?Ala?Leu?Arg?Ala?Asp?His?Asp?Ala?Leu?Leu?Gln?Asp?Asn?His?Arg

145?????????????????150?????????????????155?????????????????160

Leu?Arg?Ser?Gln?Val?Val?Thr?Leu?Thr?Glu?Lys?Met?Gln?Asp?Lys?Glu

165?????????????????170?????????????????175

Ala?Pro?Glu?Gly?Ser?Phe?Gly?Ala?Ala?Ala?Asp?Ala?Ser?Glu?Pro?Glu

180?????????????????185?????????????????190

Gln?Ala?Ala?Ala?Glu?Ala?Lys?Ala?Ser?Leu?Ala?Asp?Ala?Glu?Glu?Gln

195?????????????????200?????????????????205

Ala?Ala?Ala?Ala?Glu?Ala?Phe?Glu?Val?Val?Gln?Gln?Gln?Leu?His?Val

210?????????????????215?????????????????220

Lys?Asp?Glu?Glu?Arg?Leu?Ser?Pro?Gly?Ser?Gly?Gly?Ser?Ala?Val?Leu

225?????????????????230?????????????????235?????????????????240

Asp?Ala?Arg?Asp?Ala?Leu?Leu?Gly?Ser?Gly?Cys?Gly?Leu?Ala?Gly?Val

245?????????????????250?????????????????255

Val?Asp?Ser?Ser?Val?Asp?Ser?Tyr?Cys?Phe?Pro?Gly?Gly?Ala?Gly?Gly

260?????????????????265?????????????????270

Asp?Glu?Tyr?His?Glu?Cys?Val?Val?Gly?Pro?Val?Ala?Gly?Gly?Ile?Gln

275?????????????????280?????????????????285

Ser?Glu?Glu?Asp?Asp?Gly?Ala?Gly?Ser?Asp?Glu?Gly?Cys?Ser?Tyr?Tyr

290?????????????????295?????????????????300

Pro?Asp?Asp?Ala?Ala?Val?Phe?Phe?Ala?Ala?Ala?Gln?Gly?His?Gly?His

305?????????????????310?????????????????315?????????????????320

His?Arg?Thr?Asp?Asp?Asp?Asp?Gln?Gln?Asp?Asp?Gly?Gln?Ile?Ser?Tyr

325?????????????????330?????????????????335

Trp?Met?Trp?Asn

340

 

<210>17

<211>534

<212>DNA

＜213〉colea

 

<220>

<221>CDS

<222>(1)..(534)

＜223〉contain the zinc finger protein matter (BN47310186) of A20 structural domain and AN1 structural domain

 

<400>17

atg?gac?cac?gac?aaa?aca?gga?tgc?caa?agc?cca?cct?gaa?ggt?ccc?aag?????48

Met?Asp?His?Asp?Lys?Thr?Gly?Cys?Gln?Ser?Pro?Pro?Glu?Gly?Pro?Lys

1???????????????5???????????????????10??????????????????15

cta?tgc?atc?aac?aac?tgc?ggt?ttc?ttc?gga?agc?gct?gcc?aca?atg?aac?????96

Leu?Cys?Ile?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Ala?Ala?Thr?Met?Asn

20??????????????????25??????????????????30

atg?tgt?tcc?aag?tgt?cac?aag?gct?atc?ctg?ttt?caa?cag?gaa?cag?ggg????144

Met?Cys?Ser?Lys?Cys?His?Lys?Ala?Ile?Leu?Phe?Gln?Gln?Glu?Gln?Gly

35??????????????????40??????????????????45

gct?agg?ttt?gca?tct?gca?gtg?tct?ggt?ggt?aca?tca?tca?tcc?agc?aac????192

Ala?Arg?Phe?Ala?Ser?Ala?Val?Ser?Gly?Gly?Thr?Ser?Ser?Ser?Ser?Asn

50??????????????????55??????????????????60

atc?tta?aag?gaa?acc?ttt?gct?gct?acc?gcg?ctg?gtt?gat?gct?gaa?acc????240

Ile?Leu?Lys?Glu?Thr?Phe?Ala?Ala?Thr?Ala?Leu?Val?Asp?Ala?Glu?Thr

65??????????????????70??????????????????75??????????????????80

aaa?tcc?gtt?gag?ccg?gtg?gct?gtc?tct?gta?cag?cca?tct?tct?gtc?caa????288

Lys?Ser?Val?Glu?Pro?Val?Ala?Val?Ser?Val?Gln?Pro?Ser?Ser?Val?Gln

85??????????????????90??????????????????95

gtt?gcc?gca?gag?gta?gta?gct?cca?gaa?gcc?gct?gca?gca?aaa?cta?aag????336

Val?Ala?Ala?Glu?Val?Val?Ala?Pro?Glu?Ala?Ala?Ala?Ala?Lys?Leu?Lys

100?????????????????105?????????????????110

gaa?gga?cca?agc?cga?tgt?gct?act?tgc?aat?aaa?cgg?gtt?ggt?ctg?act????384

Glu?Gly?Pro?Ser?Arg?Cys?Ala?Thr?Cys?Asn?Lys?Arg?Val?Gly?Leu?Thr

115?????????????????120?????????????????125

gga?ttc?aaa?tgt?cgc?tgt?ggt?gac?ctc?ttc?tgc?ggg?acg?cac?cgt?tat????432

Gly?Phe?Lys?Cys?Arg?Cys?Gly?Asp?Leu?Phe?Cys?Gly?Thr?His?Arg?Tyr

130?????????????????135?????????????????140

gca?gac?ata?cac?aac?tgc?tcc?ttc?aat?tac?cat?gcc?gct?gcg?caa?gaa????480

Ala?Asp?Ile?His?Asn?Cys?Ser?Phe?Asn?Tyr?His?Ala?Ala?Ala?Gln?Glu

145?????????????????150?????????????????155?????????????????160

gct?ata?gct?aaa?gca?aac?ccg?gtt?gtg?aag?gca?gag?aag?ctt?gac?aaa????528

Ala?Ile?Ala?Lys?Ala?Asn?Pro?Val?Val?Lys?Ala?Glu?Lys?Leu?Asp?Lys

165?????????????????170?????????????????175

atc?tga????????????????????????????????????????????????????????????534

Ile

 

<210>18

<211>177

<212>PRT

＜213〉colea

 

<400>18

 

Met?Asp?His?Asp?Lys?Thr?Gly?Cys?Gln?Ser?Pro?Pro?Glu?Gly?Pro?Lys

1???????????????5???????????????????10??????????????????15

Leu?Cys?Ile?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Ala?Ala?Thr?Met?Asn

20??????????????????25??????????????????30

Met?Cys?Ser?Lys?Cys?His?Lys?Ala?Ile?Leu?Phe?Gln?Gln?Glu?Gln?Gly

35??????????????????40??????????????????45

Ala?Arg?Phe?Ala?Ser?Ala?Val?Ser?Gly?Gly?Thr?Ser?Ser?Ser?Ser?Asn

50??????????????????55??????????????????60

Ile?Leu?Lys?Glu?Thr?Phe?Ala?Ala?Thr?Ala?Leu?Val?Asp?Ala?Glu?Thr

65??????????????????70??????????????????75??????????????????80

Lys?Ser?Val?Glu?Pro?Val?Ala?Val?Ser?Val?Gln?Pro?Ser?Ser?Val?Gln

85??????????????????90??????????????????95

Val?Ala?Ala?Glu?Val?Val?Ala?Pro?Glu?Ala?Ala?Ala?Ala?Lys?Leu?Lys

100?????????????????105?????????????????110

Glu?Gly?Pro?Ser?Arg?Cys?Ala?Thr?Cys?Asn?Lys?Arg?Val?Gly?Leu?Thr

115?????????????????120?????????????????125

Gly?Phe?Lys?Cys?Arg?Cys?Gly?Asp?Leu?Phe?Cys?Gly?Thr?His?Arg?Tyr

130?????????????????135?????????????????140

Ala?Asp?Ile?His?Asn?Cys?Ser?Phe?Asn?Tyr?His?Ala?Ala?Ala?Gln?Glu

145?????????????????150?????????????????155?????????????????160

Ala?Ile?Ala?Lys?Ala?Asn?Pro?Val?Val?Lys?Ala?Glu?Lys?Leu?Asp?Lys

165?????????????????170?????????????????175

Ile

 

<210>19

<211>564

<212>DNA

＜213〉colea

 

<220>

<221>CDS

<222>(1)..(564)

＜223〉contain the zinc finger protein matter (BN51359456) of A20 structural domain and AN1 structural domain

<400>19

atg?gcg?gaa?gag?cat?cga?tgc?cag?acg?ccg?gaa?ggc?cac?cgt?ctc?tgt?????48

Met?Ala?Glu?Glu?His?Arg?Cys?Gln?Thr?Pro?Glu?Gly?His?Arg?Leu?Cys

1???????????????5???????????????????10??????????????????15

gct?aac?aac?tgc?ggc?ttc?ctc?ggc?agc?tcc?gcc?acc?atg?aat?cta?tgc?????96

Ala?Asn?Asn?Cys?Gly?Phe?Leu?Gly?Ser?Ser?Ala?Thr?Met?Asn?Leu?Cys

20??????????????????25??????????????????30

tcc?aac?tgc?tac?ggc?gat?ctc?tgc?ctt?aag?caa?cag?caa?gct?tcc?atg????144

Ser?Asn?Cys?Tyr?Gly?Asp?Leu?Cys?Leu?Lys?Gln?Gln?Gln?Ala?Ser?Met

35??????????????????40??????????????????45

aaa?tcc?acc?gtc?gaa?tcc?tct?ctc?tcc?gcc?gta?tct?cct?ccg?tcg?tca????192

Lys?Ser?Thr?Val?Glu?Ser?Ser?Leu?Ser?Ala?Val?Ser?Pro?Pro?Ser?Ser

50??????????????????55??????????????????60

gag?atc?ggc?tct?atg?caa?tcc?acc?gtt?gaa?tcc?tct?ctc?tcc?gac?gta????240

Glu?Ile?Gly?Ser?Met?Gln?Ser?Thr?Val?Glu?Ser?Ser?Leu?Ser?Asp?Val

65??????????????????70??????????????????75??????????????????80

tct?cct?cca?tca?ccg?gag?acc?att?tcc?atc?tcc?tct?cca?atg?atc?cag????288

Ser?Pro?Pro?Ser?Pro?Glu?Thr?Ile?Ser?Ile?Ser?Ser?Pro?Met?Ile?Gln

85??????????????????90??????????????????95

cct?ctc?gtt?cga?aac?cca?tca?gct?gaa?ttg?gag?gta?acg?gcg?acg?aag????336

Pro?Leu?Val?Arg?Asn?Pro?Ser?Ala?Glu?Leu?Glu?Val?Thr?Ala?Thr?Lys

100?????????????????105?????????????????110

acg?gtg?act?ccg?ccg?ccg?gag?cag?cag?cag?aaa?cgg?ccg?aat?cgg?tgc????384

Thr?Val?Thr?Pro?Pro?Pro?Glu?Gln?Gln?Gln?Lys?Arg?Pro?Asn?Arg?Cys

115?????????????????120?????????????????125

acg?acg?tgt?agg?aaa?cgg?gtc?ggg?ttg?acc?ggg?ttc?aag?tgc?cgg?tgc????432

Thr?Thr?Cys?Arg?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Lys?Cys?Arg?Cys

130?????????????????135?????????????????140

ggg?acg?act?ttt?tgc?ggg?gct?cac?agg?tac?ccg?gag?gtc?cat?gga?tgc????480

Gly?Thr?Thr?Phe?Cys?Gly?Ala?His?Arg?Tyr?Pro?Glu?Val?His?Gly?Cys

145?????????????????150?????????????????155?????????????????160

acc?ttc?gat?ttc?aaa?tcg?gcc?ggt?cgc?gaa?gag?atc?gcc?aag?gcg?aac????528

Thr?Phe?Asp?Phe?Lys?Ser?Ala?Gly?Arg?Glu?Glu?Ile?Ala?Lys?Ala?Asn

165?????????????????170?????????????????175

cca?ctc?gtc?aaa?gcg?gcg?aag?ctt?cag?aag?att?tga??????????????????????64

Pro?Leu?Val?Lys?Ala?Ala?Lys?Leu?Gln?Lys?Ile

180?????????????????185

 

<210>20

<211>187

<212>PRT

＜213〉colea

 

<400>20

 

Met?Ala?Glu?Glu?His?Arg?Cys?Gln?Thr?Pro?Glu?Gly?His?Arg?Leu?Cys

1???????????????5???????????????????10??????????????????15

Ala?Asn?Asn?Cys?Gly?Phe?Leu?Gly?Ser?Ser?Ala?Thr?Met?Asn?Leu?Cys

20??????????????????25??????????????????30

Ser?Asn?Cys?Tyr?Gly?Asp?Leu?Cys?Leu?Lys?Gln?Gln?Gln?Ala?Ser?Met

35??????????????????40??????????????????45

Lys?Ser?Thr?Val?Glu?Ser?Ser?Leu?Ser?Ala?Val?Ser?Pro?Pro?Ser?Ser

50??????????????????55??????????????????60

Glu?Ile?Gly?Ser?Met?Gln?Ser?Thr?Val?Glu?Ser?Ser?Leu?Ser?Asp?Val

65??????????????????70??????????????????75??????????????????80

Ser?Pro?Pro?Ser?Pro?Glu?Thr?Ile?Ser?Ile?Ser?Ser?Pro?Met?Ile?Gln

85??????????????????90??????????????????95

Pro?Leu?Val?Arg?Asn?Pro?Ser?Ala?Glu?Leu?Glu?Val?Thr?Ala?Thr?Lys

100?????????????????105?????????????????110

Thr?Val?Thr?Pro?Pro?Pro?Glu?Gln?Gln?Gln?Lys?Arg?Pro?Asn?Arg?Cys

115?????????????????120?????????????????125

Thr?Thr?Cys?Arg?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Lys?Cys?Arg?Cys

130?????????????????135?????????????????140

Gly?Thr?Thr?Phe?Cys?Gly?Ala?His?Arg?Tyr?Pro?Glu?Val?His?Gly?Cys

145?????????????????150?????????????????155?????????????????160

Thr?Phe?Asp?Phe?Lys?Ser?Ala?Gly?Arg?Glu?Glu?Ile?Ala?Lys?Ala?Asn

165?????????????????170?????????????????175

Pro?Leu?Val?Lys?Ala?Ala?Lys?Leu?Gln?Lys?Ile

180?????????????????185

 

<210>21

<211>465

<212>DNA

＜213〉barley

 

<220>

<221>CDS

<222>(1)..(465)

＜223〉contain the zinc finger protein matter (HV62552639) of A20 structural domain and AN1 structural domain

 

<400>21

atg?gcc?cag?gag?agt?tgt?gat?ctc?aac?aag?gac?gag?gcc?gag?atc?ctg?????48

Met?Ala?Gln?Glu?Ser?Cys?Asp?Leu?Asn?Lys?Asp?Glu?Ala?Glu?Ile?Leu

1???????????????5???????????????????10??????????????????15

aag?cca?tcc?tcc?tcc?aca?cct?tcg?cct?cct?tcg?cca?gcc?aca?cca?cca?????96

Lys?Pro?Ser?Ser?Ser?Thr?Pro?Ser?Pro?Pro?Ser?Pro?Ala?Thr?Pro?Pro

20??????????????????25??????????????????30

cca?cca?acc?gct?caa?ata?cca?gaa?cca?caa?cct?cca?cac?tca?cca?cca????144

Pro?Pro?Thr?Ala?Gln?Ile?Pro?Glu?Pro?Gln?Pro?Pro?His?Ser?Pro?Pro

35??????????????????40??????????????????45

caa?cca?ccg?gca?gct?caa?ttc?ttg?tcc?agg?ccc?tgc?gag?gtt?gtt?ccc????192

Gln?Pro?Pro?Ala?Ala?Gln?Phe?Leu?Ser?Arg?Pro?Cys?Glu?Val?Val?Pro

50??????????????????55??????????????????60

ata?gag?act?tcc?aaa?aag?agg?aaa?cat?gct?gat?gcg?gtg?tca?atg?gcc????240

Ile?Glu?Thr?Ser?Lys?Lys?Arg?Lys?His?Ala?Asp?Ala?Val?Ser?Met?Ala

65??????????????????70??????????????????75??????????????????80

att?gtg?gtt?gag?cca?ttg?tcg?tct?gtg?ctg?ttc?gtt?aac?cgt?tgc?aac????288

Ile?Val?Val?Glu?Pro?Leu?Ser?Ser?Val?Leu?Phe?Val?Asn?Arg?Cys?Asn

85??????????????????90??????????????????95

gtg?tgc?cgc?aag?aga?gtt?ggt?ttg?acc?ggg?ttc?cgt?tgc?cgg?tgt?gag????336

Val?Cys?Arg?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Arg?Cys?Arg?Cys?Glu

100?????????????????105?????????????????110

aag?ctc?ttt?tgt?ccg?cgc?cac?cgg?cat?tca?gaa?agc?cac?gac?tgc?tca????384

Lys?Leu?Phe?Cys?Pro?Arg?His?Arg?His?Ser?Glu?Ser?His?Asp?Cys?Ser

115?????????????????120?????????????????125

ttt?gat?tat?aaa?act?gtg?ggt?cgg?gag?gag?att?gcc?cgg?gca?aac?cct????432

Phe?Asp?Tyr?Lys?Thr?Val?Gly?Arg?Glu?Glu?Ile?Ala?Arg?Ala?Asn?Pro

130?????????????????135?????????????????140

ctg?atc?agg?gct?gcc?aag?atc?att?agg?ata?tga????????????????????????465

Leu?Ile?Arg?Ala?Ala?Lys?Ile?Ile?Arg?Ile

145?????????????????150

 

<210>22

<211>154

<212>PRT

＜213〉barley

 

<400>22

 

Met?Ala?Gln?Glu?Ser?Cys?Asp?Leu?Asn?Lys?Asp?Glu?Ala?Glu?Ile?Leu

1???????????????5???????????????????10??????????????????15

Lys?Pro?Ser?Ser?Ser?Thr?Pro?Ser?Pro?Pro?Ser?Pro?Ala?Thr?Pro?Pro

20??????????????????25??????????????????30

Pro?Pro?Thr?Ala?Gln?Ile?Pro?Glu?Pro?Gln?Pro?Pro?His?Ser?Pro?Pro

35??????????????????40??????????????????45

Gln?Pro?Pro?Ala?Ala?Gln?Phe?Leu?Ser?Arg?Pro?Cys?Glu?Val?Val?Pro

50??????????????????55??????????????????60

Ile?Glu?Thr?Ser?Lys?Lys?Arg?Lys?His?Ala?Asp?Ala?Val?Ser?Met?Ala

65??????????????????70??????????????????75??????????????????80

Ile?Val?Val?Glu?Pro?Leu?Ser?Ser?Val?Leu?Phe?Val?Asn?Arg?Cys?Asn

85??????????????????90??????????????????95

Val?Cys?Arg?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Arg?Cys?Arg?Cys?Glu

100?????????????????105?????????????????110

Lys?Leu?Phe?Cys?Pro?Arg?His?Arg?His?Ser?Glu?Ser?His?Asp?Cys?Ser

115?????????????????120?????????????????125

Phe?Asp?Tyr?Lys?Thr?Val?Gly?Arg?Glu?Glu?Ile?Ala?Arg?Ala?Asn?Pro

130?????????????????135?????????????????140

Leu?Ile?Arg?Ala?Ala?Lys?Ile?Ile?Arg?Ile

145?????????????????150

 

<210>23

<211>516

<212>DNA

＜213〉corn

 

<220>

<221>CDS

<222>(1)..(516)

＜223〉contain the zinc finger protein matter (ZM61995511) of A20 structural domain and AN1 structural domain

 

<400>23

atg?gaa?cac?aag?gag?gcg?ggc?tgc?cag?cag?ccg?gag?ggc?cca?atc?cta?????48

Met?Glu?His?Lys?Glu?Ala?Gly?Cys?Gln?Gln?Pro?Glu?Gly?Pro?Ile?Leu

1???????????????5???????????????????10??????????????????15

tgc?atc?aat?aac?tgc?ggc?ttc?ttc?ggc?agt?gct?gcg?acg?atg?aac?atg?????96

Cys?Ile?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Ala?Ala?Thr?Met?Asn?Met

20??????????????????25??????????????????30

tgc?tcc?aag?tgc?cac?aag?gag?atg?ata?acg?aag?cag?gag?cag?gcc?cag????144

Cys?Ser?Lys?Cys?His?Lys?Glu?Met?Ile?Thr?Lys?Gln?Glu?Gln?Ala?Gln

35??????????????????40??????????????????45

ctg?gct?gcc?tcc?ccc?atc?gat?agc?att?gtc?aat?ggc?ggt?gac?ggc?ggg????192

Leu?Ala?Ala?Ser?Pro?Ile?Asp?Ser?Ile?Val?Asn?Gly?Gly?Asp?Gly?Gly

50??????????????????55??????????????????60

aaa?gga?cct?gta?att?gct?gca?tct?gta?aat?gtg?gca?gtt?cct?caa?gtt????240

Lys?Gly?Pro?Val?Ile?Ala?Ala?Ser?Val?Asn?Val?Ala?Val?Pro?Gln?Val

65??????????????????70??????????????????75??????????????????80

gag?cag?aag?act?att?gtt?gtg?cag?ccc?atg?ctt?gta?gct?gaa?acc?agc????288

Glu?Gln?Lys?Thr?Ile?Val?Val?Gln?Pro?Met?Leu?Val?Ala?Glu?Thr?Ser

85??????????????????90??????????????????95

gag?gct?gct?gct?gta?atc?ccc?aag?gcc?aag?gaa?ggc?cca?gac?cgg?tgc????336

Glu?Ala?Ala?Ala?Val?Ile?Pro?Lys?Ala?Lys?Glu?Gly?Pro?Asp?Arg?Cys

100?????????????????105?????????????????110

gcg?gcc?tgc?agg?aag?cgt?gtt?ggg?ctg?acg?gga?ttt?agc?tgc?cga?tgc????384

Ala?Ala?Cys?Arg?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Ser?Cys?Arg?Cys

115?????????????????120?????????????????125

ggg?aac?atg?tac?tgt?tcg?gtg?cac?cgc?tac?tcc?gac?aaa?cat?gac?tgt????432

Gly?Asn?Met?Tyr?Cys?Ser?Val?His?Arg?Tyr?Ser?Asp?Lys?His?Asp?Cys

130?????????????????135?????????????????140

cag?ttc?gac?tat?cgg?act?gca?gca?agg?gac?gcg?att?gcc?aag?gcc?aat????480

Gln?Phe?Asp?Tyr?Arg?Thr?Ala?Ala?Arg?Asp?Ala?Ile?Ala?Lys?Ala?Asn

145?????????????????150?????????????????155?????????????????160

cct?gtg?gtg?agg?gcg?gag?aag?ctc?gac?aag?atc?tga????????????????????516

Pro?Val?Val?Arg?Ala?Glu?Lys?Leu?Asp?Lys?Ile

165?????????????????170

 

<210>24

<211>171

<212>PRT

＜213〉corn

 

<400>24

 

Met?Glu?His?Lys?Glu?Ala?Gly?Cys?Gln?Gln?Pro?Glu?Gly?Pro?Ile?Leu

1???????????????5???????????????????10??????????????????15

Cys?Ile?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Ala?Ala?Thr?Met?Asn?Met

20??????????????????25??????????????????30

Cys?Ser?Lys?Cys?His?Lys?Glu?Met?Ile?Thr?Lys?Gln?Glu?Gln?Ala?Gln

35??????????????????40??????????????????45

Leu?Ala?Ala?Ser?Pro?Ile?Asp?Ser?Ile?Val?Asn?Gly?Gly?Asp?Gly?Gly

50??????????????????55??????????????????60

Lys?Gly?Pro?Val?Ile?Ala?Ala?Ser?Val?Asn?Val?Ala?Val?Pro?Gln?Val

65??????????????????70??????????????????75??????????????????80

Glu?Gln?Lys?Thr?Ile?Val?Val?Gln?Pro?Met?Leu?Val?Ala?Glu?Thr?Ser

85??????????????????90??????????????????95

Glu?Ala?Ala?Ala?Val?Ile?Pro?Lys?Ala?Lys?Glu?Gly?Pro?Asp?Arg?Cys

100?????????????????105?????????????????110

Ala?Ala?Cys?Arg?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Ser?Cys?Arg?Cys

115?????????????????120?????????????????125

Gly?Asn?Met?Tyr?Cys?Ser?Val?His?Arg?Tyr?Ser?Asp?Lys?His?Asp?Cys

130?????????????????135?????????????????140

Gln?Phe?Asp?Tyr?Arg?Thr?Ala?Ala?Arg?Asp?Ala?Ile?Ala?Lys?Ala?Asn

145?????????????????150?????????????????155?????????????????160

Pro?Val?Val?Arg?Ala?Glu?Lys?Leu?Asp?Lys?Ile

165?????????????????170

 

<210>25

<211>720

<212>DNA

＜213〉flax

 

<220>

<221>CDS

<222>(1)..(720)

＜223〉contain the zinc finger protein matter (LU61567101) of A20 structural domain and AN1 structural domain

 

<400>25

atg?gct?cct?tca?cct?tgc?gtc?cac?ggc?tgc?acg?gcc?aat?tgc?ccc?cgc?????48

Met?Ala?Pro?Ser?Pro?Cys?Val?His?Gly?Cys?Thr?Ala?Asn?Cys?Pro?Arg

1???????????????5???????????????????10??????????????????15

tgc?cac?tct?tac?gga?cac?ccc?atc?ttc?ggg?aac?tca?gat?ctc?gcc?gct?????96

Cys?His?Ser?Tyr?Gly?His?Pro?Ile?Phe?Gly?Asn?Ser?Asp?Leu?Ala?Ala

20??????????????????25??????????????????30

ggc?ggc?agc?gat?acg?tcc?acg?tcg?gtg?ttt?gga?aaa?gta?gga?tcc?gtc????144

Gly?Gly?Ser?Asp?Thr?Ser?Thr?Ser?Val?Phe?Gly?Lys?Val?Gly?Ser?Val

35??????????????????40??????????????????45

gtg?att?cag?tcg?cct?gcg?aag?aat?cac?gcg?ttc?ggc?caa?gct?tgt?ggc????192

Val?Ile?Gln?Ser?Pro?Ala?Lys?Asn?His?Ala?Phe?Gly?Gln?Ala?Cys?Gly

50??????????????????55??????????????????60

ccg?gtt?ttt?ccc?tcg?agc?tcc?tcc?cct?ttc?cgc?cgc?atc?aag?ttc?ggc????240

Pro?Val?Phe?Pro?Ser?Ser?Ser?Ser?Pro?Phe?Arg?Arg?Ile?Lys?Phe?Gly

65??????????????????70??????????????????75??????????????????80

ccc?aaa?gat?ggc?gag?ggg?aaa?gga?ccg?ctg?aag?ccg?atc?gag?aag?cag????288

Pro?Lys?Asp?Gly?Glu?Gly?Lys?Gly?Pro?Leu?Lys?Pro?Ile?Glu?Lys?Gln

85??????????????????90??????????????????95

ccg?tcg?aag?aag?cgt?ccg?ttc?tgc?ttc?tct?ccc?gac?gag?acg?att?gac????336

Pro?Ser?Lys?Lys?Arg?Pro?Phe?Cys?Phe?Ser?Pro?Asp?Glu?Thr?Ile?Asp

100?????????????????105?????????????????110

gcg?acg?gtt?cct?ccg?tcc?acc?aaa?ccg?ttc?ggt?tcg?ttc?cgt?tcc?gtc????384

Ala?Thr?Val?Pro?Pro?Ser?Thr?Lys?Pro?Phe?Gly?Ser?Phe?Arg?Ser?Val

115?????????????????120?????????????????125

tgt?gtc?acg?gac?gcc?gac?gag?gcc?agg?ttg?aag?gcg?aac?cgc?gag?ttc????432

Cys?Val?Thr?Asp?Ala?Asp?Glu?Ala?Arg?Leu?Lys?Ala?Asn?Arg?Glu?Phe

130?????????????????135?????????????????140

ttc?gct?ccg?gta?tcc?cgc?aaa?cgt?ggc?ttc?gat?ccg?act?gac?atg?acc????480

Phe?Ala?Pro?Val?Ser?Arg?Lys?Arg?Gly?Phe?Asp?Pro?Thr?Asp?Met?Thr

145?????????????????150?????????????????155?????????????????160

ttc?ggt?aac?gcc?gcc?gcc?gct?gcg?gct?aat?gcg?agg?gag?gaa?gcg?aag????528

Phe?Gly?Asn?Ala?Ala?Ala?Ala?Ala?Ala?Asn?Ala?Arg?Glu?Glu?Ala?Lys

165?????????????????170?????????????????175

aag?tgg?tgc?ggc?agt?tgc?aag?aag?cgc?gtg?ggg?ctg?tta?ggg?ttc?aag????576

Lys?Trp?Cys?Gly?Ser?Cys?Lys?Lys?Arg?Val?Gly?Leu?Leu?Gly?Phe?Lys

180?????????????????185?????????????????190

tgc?agg?tgt?acg?aag?ttc?ttc?tgt?ggg?aag?cat?cgg?tat?cct?gag?gag????624

Cys?Arg?Cys?Thr?Lys?Phe?Phe?Cys?Gly?Lys?His?Arg?Tyr?Pro?Glu?Glu

195?????????????????200?????????????????205

cat?ggt?tgt?acg?ttc?gat?cat?gtg?gcg?ttc?ggg?agg?cgg?att?atc?gag????672

His?Gly?Cys?Thr?Phe?Asp?His?Val?Ala?Phe?Gly?Arg?Arg?Ile?Ile?Glu

210?????????????????215?????????????????220

aaa?cag?aat?cct?gtt?ctc?gag?acc?gac?aag?ctg?gtg?gac?aga?atc?tga????720

Lys?Gln?Asn?Pro?Val?Leu?Glu?Thr?Asp?Lys?Leu?Val?Asp?Arg?Ile

225?????????????????230?????????????????235

<210>26

<211>239

<212>PRT

＜213〉flax

 

<400>26

 

Met?Ala?Pro?Ser?Pro?Cys?Val?His?Gly?Cys?Thr?Ala?Asn?Cys?Pro?Arg

1???????????????5???????????????????10??????????????????15

Cys?His?Ser?Tyr?Gly?His?Pro?Ile?Phe?Gly?Asn?Ser?Asp?Leu?Ala?Ala

20??????????????????25??????????????????30

Gly?Gly?Ser?Asp?Thr?Ser?Thr?Ser?Val?Phe?Gly?Lys?Val?Gly?Ser?Val

35??????????????????40??????????????????45

Val?Ile?Gln?Ser?Pro?Ala?Lys?Asn?His?Ala?Phe?Gly?Gln?Ala?Cys?Gly

50??????????????????55??????????????????60

Pro?Val?Phe?Pro?Ser?Ser?Ser?Ser?Pro?Phe?Arg?Arg?Ile?Lys?Phe?Gly

65??????????????????70??????????????????75??????????????????80

Pro?Lys?Asp?Gly?Glu?Gly?Lys?Gly?Pro?Leu?Lys?Pro?Ile?Glu?Lys?Gln

85??????????????????90??????????????????95

Pro?Ser?Lys?Lys?Arg?Pro?Phe?Cys?Phe?Ser?Pro?Asp?Glu?Thr?Ile?Asp

100?????????????????105?????????????????110

Ala?Thr?Val?Pro?Pro?Ser?Thr?Lys?Pro?Phe?Gly?Ser?Phe?Arg?Ser?Val

115?????????????????120?????????????????125

Cys?Val?Thr?Asp?Ala?Asp?Glu?Ala?Arg?Leu?Lys?Ala?Asn?Arg?Glu?Phe

130?????????????????135?????????????????140

Phe?Ala?Pro?Val?Ser?Arg?Lys?Arg?Gly?Phe?Asp?Pro?Thr?Asp?Met?Thr

145?????????????????150?????????????????155?????????????????160

Phe?Gly?Asn?Ala?Ala?Ala?Ala?Ala?Ala?Asn?Ala?Arg?Glu?Glu?Ala?Lys

165?????????????????170?????????????????175

Lys?Trp?Cys?Gly?Ser?Cys?Lys?Lys?Arg?Val?Gly?Leu?Leu?Gly?Phe?Lys

180?????????????????185?????????????????190

Cys?Arg?Cys?Thr?Lys?Phe?Phe?Cys?Gly?Lys?His?Arg?Tyr?Pro?Glu?Glu

195?????????????????200?????????????????205

His?Gly?Cys?Thr?Phe?Asp?His?Val?Ala?Phe?Gly?Arg?Arg?Ile?Ile?Glu

210?????????????????215?????????????????220

Lys?Gln?Asn?Pro?Val?Leu?Glu?Thr?Asp?Lys?Leu?Val?Asp?Arg?Ile

225?????????????????230?????????????????235

 

<210>27

<211>510

<212>DNA

＜213〉flax

 

<220>

<221>CDS

<222>(1)..(510)

＜223〉contain the zinc finger protein matter (LU61893412) of A20 structural domain and AN1 structural domain

 

<400>27

atg?gac?cat?gac?gag?gca?ggc?tgc?cag?gct?cct?tcc?gat?cat?cct?att?????48

Met?Asp?His?Asp?Glu?Ala?Gly?Cys?Gln?Ala?Pro?Ser?Asp?His?Pro?Ile

1???????????????5???????????????????10??????????????????15

ctg?tgc?gtt?aac?aat?tgc?ggc?ttc?ttc?gga?agt?gct?gcc?acc?atg?aac?????96

Leu?Cys?Val?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Ala?Ala?Thr?Met?Asn

20??????????????????25??????????????????30

atg?tgc?tca?aag?tgc?cac?aag?gat?acg?atg?cta?aac?caa?gag?caa?tcc????144

Met?Cys?Ser?Lys?Cys?His?Lys?Asp?Thr?Met?Leu?Asn?Gln?Glu?Gln?Ser

35??????????????????40??????????????????45

aag?ctt?gct?gct?tca?tcg?gca?gca?agt?atc?ctc?aac?gga?tcg?tcg?atg????192

Lys?Leu?Ala?Ala?Ser?Ser?Ala?Ala?Ser?Ile?Leu?Asn?Gly?Ser?Ser?Met

50??????????????????55??????????????????60

agc?ctc?gga?agg?gaa?ctc?gtt?att?gct?gct?aag?acc?aat?tcg?gta?gaa????240

Ser?Leu?Gly?Arg?Glu?Leu?Val?Ile?Ala?Ala?Lys?Thr?Asn?Ser?Val?Glu

65??????????????????70??????????????????75??????????????????80

ccc?aag?acc?atc?tcc?gtc?caa?cca?tct?tct?gct?tca?agt?gct?gaa?gag????288

Pro?Lys?Thr?Ile?Ser?Val?Gln?Pro?Ser?Ser?Ala?Ser?Ser?Ala?Glu?Glu

85??????????????????90??????????????????95

agt?atc?gaa?atg?aag?ctg?cca?aaa?gaa?ggg?ccc?agt?agg?tgc?aac?act????336

Ser?Ile?Glu?Met?Lys?Leu?Pro?Lys?Glu?Gly?Pro?Ser?Arg?Cys?Asn?Thr

100?????????????????105?????????????????110

tgc?aac?aaa?cgt?gtc?ggt?ttg?acc?gga?ttc?aaa?tgt?cgg?tgc?gag?aac????384

Cys?Asn?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Lys?Cys?Arg?Cys?Glu?Asn

115?????????????????120?????????????????125

atg?ttc?tgc?gca?aac?cat?cgc?tac?tcg?gac?aag?cac?aat?tgc?ccc?ttt????432

Met?Phe?Cys?Ala?Asn?His?Arg?Tyr?Ser?Asp?Lys?His?Asn?Cys?Pro?Phe

130?????????????????135?????????????????140

gat?tac?cgc?act?gct?ggc?cgt?gaa?gct?atc?tca?aag?gcc?aat?cct?ttg????480

Asp?Tyr?Arg?Thr?Ala?Gly?Arg?Glu?Ala?Ile?Ser?Lys?Ala?Asn?Pro?Leu

145?????????????????150?????????????????155?????????????????160

gtg?aag?gcg?gag?aag?ctc?gac?aaa?atc?tga????????????????????????????510

Val?Lys?Ala?Glu?Lys?Leu?Asp?Lys?Ile

165

 

<210>28

<211>169

<212>PRT

＜213〉flax

 

<400>28

 

Met?Asp?His?Asp?Glu?Ala?Gly?Cys?Gln?Ala?Pro?Ser?Asp?His?Pro?Ile

1???????????????5???????????????????10??????????????????15

Leu?Cys?Val?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Ala?Ala?Thr?Met?Asn

20??????????????????25??????????????????30

Met?Cys?Ser?Lys?Cys?His?Lys?Asp?Thr?Met?Leu?Asn?Gln?Glu?Gln?Ser

35??????????????????40??????????????????45

Lys?Leu?Ala?Ala?Ser?Ser?Ala?Ala?Ser?Ile?Leu?Asn?Gly?Ser?Ser?Met

50??????????????????55??????????????????60

Ser?Leu?Gly?Arg?Glu?Leu?Val?Ile?Ala?Ala?Lys?Thr?Asn?Ser?Val?Glu

65??????????????????70??????????????????75??????????????????80

Pro?Lys?Thr?Ile?Ser?Val?Gln?Pro?Ser?Ser?Ala?Ser?Ser?Ala?Glu?Glu

85??????????????????90??????????????????95

Ser?Ile?Glu?Met?Lys?Leu?Pro?Lys?Glu?Gly?Pro?Ser?Arg?Cys?Asn?Thr

100?????????????????105?????????????????110

Cys?Asn?Lys?Arg?Val?Gly?Leu?Thr?GIy?Phe?Lys?Cys?Arg?Cys?Glu?Asn

115?????????????????120?????????????????125

Met?Phe?Cys?Ala?Asn?His?Arg?Tyr?Ser?Asp?Lys?His?Asn?Cys?Pro?Phe

130?????????????????135?????????????????140

Asp?Tyr?Arg?Thr?Ala?Gly?Arg?Glu?Ala?Ile?Ser?Lys?Ala?Asn?Pro?Leu

145?????????????????150?????????????????155?????????????????160

Val?Lys?Ala?Glu?Lys?Leu?Asp?Lys?Ile

165

 

<210>29

<211>495

<212>DNA

＜213〉rice

 

<220>

<221>CDS

<222>(1)..(495)

＜223〉contain the zinc finger protein matter (OS39781852) of A20 structural domain and AN1 structural domain

 

<400>29

atg?gcg?cag?cgc?gac?aag?aag?gat?cag?gag?ccg?acg?gag?ctc?agg?gcg?????48

Met?Ala?Gln?Arg?Asp?Lys?Lys?Asp?Gln?Glu?Pro?Thr?Glu?Leu?Arg?Ala

1???????????????5???????????????????10??????????????????15

ccg?gag?atc?acg?ctg?tgc?gcc?aac?agc?tgc?gga?ttc?ccg?ggc?aac?ccg?????96

Pro?Glu?Ile?Thr?Leu?Cys?Ala?Asn?Ser?Cys?Gly?Phe?Pro?Gly?Asn?Pro

20??????????????????25??????????????????30

gcc?acg?cag?aac?ctc?tgc?cag?aac?tgc?ttc?ttg?gcg?gcc?acg?gcg?tcc????144

Ala?Thr?Gln?Asn?Leu?Cys?Gln?Asn?Cys?Phe?Leu?Ala?Ala?Thr?Ala?Ser

35??????????????????40??????????????????45

acc?tcg?tcg?ccg?tct?tct?ttg?tcg?tca?ccg?gtg?ctc?gac?aag?cag?ccg????192

Thr?Ser?Ser?Pro?Ser?Ser?Leu?Ser?Ser?Pro?Val?Leu?Asp?Lys?Gln?Pro

50??????????????????55??????????????????60

ccg?agg?ccg?gcg?gcg?ccg?ctg?gtt?gag?cct?cag?gct?cct?ctc?cca?ccg????240

Pro?Arg?Pro?Ala?Ala?Pro?Leu?Val?Glu?Pro?Gln?Ala?Pro?Leu?Pro?Pro

65??????????????????70??????????????????75??????????????????80

cct?gtg?gag?gag?atg?gcc?tcc?gcg?ctc?gcg?acg?gcg?ccg?gcg?ccg?gtc????288

Pro?Val?Glu?Glu?Met?Ala?Ser?Ala?Leu?Ala?Thr?Ala?Pro?Ala?Pro?Val

85??????????????????90??????????????????95

gcc?aag?acg?tcg?gcg?gtg?aac?cgg?tgc?tcc?agg?tgc?cgg?aag?cgt?gtc????336

Ala?Lys?Thr?Ser?Ala?Val?Asn?Arg?Cys?Ser?Arg?Cys?Arg?Lys?Arg?Val

100?????????????????105?????????????????110

ggc?ctc?acc?ggg?ttc?cgg?tgc?cgg?tgc?ggc?cac?ctg?ttc?tgc?ggc?gag????384

Gly?Leu?Thr?Gly?Phe?Arg?Cys?Arg?Cys?Gly?His?Leu?Phe?Cys?Gly?Glu

115?????????????????120?????????????????125

cac?cgg?tac?tcc?gac?cgc?cac?ggc?tgc?agc?tac?gac?tac?aag?tcg?gcg????432

His?Arg?Tyr?Ser?Asp?Arg?His?Gly?Cys?Ser?Tyr?Asp?Tyr?Lys?Ser?Ala

130?????????????????135?????????????????140

gcg?agg?gac?gcc?atc?gcc?agg?gac?aac?ccg?gtg?gtg?cgc?gcg?gcc?aag????480

Ala?Arg?Asp?Ala?Ile?Ala?Arg?Asp?Asn?Pro?Val?Val?Arg?Ala?Ala?Lys

145?????????????????150?????????????????155?????????????????160

atc?gtt?agg?ttc?tga????????????????????????????????????????????????495

Ile?Val?Arg?Phe

 

<210>30

<211>164

<212>PRT

＜213〉rice

 

<400>30

 

Met?Ala?Gln?Arg?Asp?Lys?Lys?Asp?Gln?Glu?Pro?Thr?Glu?Leu?Arg?Ala

1???????????????5???????????????????10??????????????????15

Pro?Glu?Ile?Thr?Leu?Cys?Ala?Asn?Ser?Cys?Gly?Phe?Pro?Gly?Asn?Pro

20??????????????????25??????????????????30

Ala?Thr?Gln?Asn?Leu?Cys?Gln?Asn?Cys?Phe?Leu?Ala?Ala?Thr?Ala?Ser

35??????????????????40??????????????????45

Thr?Ser?Ser?Pro?Ser?Ser?Leu?Ser?Ser?Pro?Val?Leu?Asp?Lys?Gln?Pro

50??????????????????55??????????????????60

Pro?Arg?Pro?Ala?Ala?Pro?Leu?Val?Glu?Pro?Gln?Ala?Pro?Leu?Pro?Pro

65??????????????????70??????????????????75??????????????????80

Pro?Val?Glu?Glu?Met?Ala?Ser?Ala?Leu?Ala?Thr?Ala?Pro?Ala?Pro?Val

85??????????????????90??????????????????95

Ala?Lys?Thr?Ser?Ala?Val?Asn?Arg?Cys?Ser?Arg?Cys?Arg?Lys?Arg?Val

100?????????????????105?????????????????110

Gly?Leu?Thr?Gly?Phe?Arg?Cys?Arg?Cys?Gly?His?Leu?Phe?Cys?Gly?Glu

115?????????????????120?????????????????125

His?Arg?Tyr?Ser?Asp?Arg?His?Gly?Cys?Ser?Tyr?Asp?Tyr?Lys?Ser?Ala

130?????????????????135?????????????????140

Ala?Arg?Asp?Ala?Ile?Ala?Arg?Asp?Asn?Pro?Val?Val?Arg?Ala?Ala?Lys

145?????????????????150?????????????????155?????????????????160

Ile?Val?Arg?Phe

 

<210>31

<211>495

<212>DNA

＜213〉rice

 

<220>

<221>CDS

<222>(1)..(495)

＜223〉contain the zinc finger protein matter (OS34701560) of A20 structural domain and AN1 structural domain

 

<400>31

atg?gcc?gaa?gaa?cac?cga?tgc?caa?gct?ccc?gaa?ggt?cac?aga?ctc?tgc????48

Met?Ala?Glu?Glu?His?Arg?Cys?Gln?Ala?Pro?Glu?Gly?His?Arg?Leu?Cys

1???????????????5???????????????????10??????????????????15

tcc?aac?aac?tgc?ggt?ttc?ttt?ggt?agc?ccc?gcc?acc?atg?aat?ctc?tgt?????96

Ser?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Pro?Ala?Thr?Met?Asn?Leu?Cys

20??????????????????25??????????????????30

tcc?aaa?tgc?tac?aga?gac?atc?cgt?ttg?aag?gaa?gaa?gaa?caa?gcc?aaa????144

Ser?Lys?Cys?Tyr?Arg?Asp?Ile?Arg?Leu?Lys?Glu?Glu?Glu?Gln?Ala?Lys

35??????????????????40??????????????????45

acc?aaa?tcc?aca?atc?gaa?acc?gct?ctt?tca?gga?tct?tcc?tcc?gcc?acc????192

Thr?Lys?Ser?Thr?Ile?Glu?Thr?Ala?Leu?Ser?Gly?Ser?Ser?Ser?Ala?Thr

50??????????????????55??????????????????60

gtc?acc?gca?acc?gcc?gtc?gtt?gcc?tcc?tcc?gtg?gaa?tcc?cct?tcg?gcg????240

Val?Thr?Ala?Thr?Ala?Val?Val?Ala?Ser?Ser?Val?Glu?Ser?Pro?Ser?Ala

65??????????????????70??????????????????75??????????????????80

ccg?gtt?gaa?tcc?ctc?cct?caa?cca?ccg?gtg?ctg?att?tcg?ccg?gat?ata????288

Pro?Val?Glu?Ser?Leu?Pro?Gln?Pro?Pro?Val?Leu?Ile?Ser?Pro?Asp?Ile

85??????????????????90??????????????????95

gcc?gca?ccg?gtt?cag?gcg?aac?cgg?tgc?ggc?gcg?tgt?agg?aag?cgc?gtg????336

Ala?Ala?Pro?Val?Gln?Ala?Asn?Arg?Cys?Gly?Ala?Cys?Arg?Lys?Arg?Val

100?????????????????105?????????????????110

ggg?ttg?aca?ggg?ttc?aag?tgc?agg?tgc?gga?aca?acg?ttt?tgt?ggg?agc????384

Gly?Leu?Thr?Gly?Phe?Lys?Cys?Arg?Cys?Gly?Thr?Thr?Phe?Cys?Gly?Ser

115?????????????????120?????????????????125

cac?agg?tac?ccc?gag?aaa?cac?gcg?tgt?ggc?ttc?gat?ttc?aag?gcg?gtg????432

His?Arg?Tyr?Pro?Glu?Lys?His?Ala?Cys?Gly?Phe?Asp?Phe?Lys?Ala?Val

130?????????????????135?????????????????140

ggg?aga?gag?gag?ata?gca?cgg?gcg?aat?ccc?gtg?atc?aaa?ggc?gag?aag????480

Gly?Arg?Glu?Glu?Ile?Ala?Arg?Ala?Asn?Pro?Val?Ile?Lys?Gly?Glu?Lys

145?????????????????150?????????????????155?????????????????160

cta?cgg?agg?att?taa????????????????????????????????????????????????495

Leu?Arg?Arg?Ile

 

<210>32

<211>164

<212>PRT

＜213〉rice

 

<400>32

 

Met?Ala?Glu?Glu?His?Arg?Cys?Gln?Ala?Pro?Glu?Gly?His?Arg?Leu?Cys

1???????????????5???????????????????10??????????????????15

Ser?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Pro?Ala?Thr?Met?Asn?Leu?Cys

20??????????????????25??????????????????30

Ser?Lys?Cys?Tyr?Arg?Asp?Ile?Arg?Leu?Lys?Glu?Glu?Glu?Gln?Ala?Lys

35??????????????????40??????????????????45

Thr?Lys?Ser?Thr?Ile?Glu?Thr?Ala?Leu?Ser?Gly?Ser?Ser?Ser?Ala?Thr

50??????????????????55??????????????????60

Val?Thr?Ala?Thr?Ala?Val?Val?Ala?Ser?Ser?Val?Glu?Ser?Pro?Ser?Ala

65??????????????????70??????????????????75??????????????????80

Pro?Val?Glu?Ser?Leu?Pro?Gln?Pro?Pro?Val?Leu?Ile?Ser?Pro?Asp?Ile

85??????????????????90??????????????????95

Ala?Ala?Pro?Val?Gln?Ala?Asn?Arg?Cys?Gly?Ala?Cys?Arg?Lys?Arg?Val

100?????????????????105?????????????????110

Gly?Leu?Thr?Gly?Phe?Lys?Cys?Arg?Cys?Gly?Thr?Thr?Phe?Cys?Gly?Ser

115?????????????????120?????????????????125

His?Arg?Tyr?Pro?Glu?Lys?His?Ala?Cys?Gly?Phe?Asp?Phe?Lys?Ala?Val

130?????????????????135?????????????????140

Gly?Arg?Glu?Glu?Ile?Ala?Arg?Ala?Asn?Pro?Val?Ile?Lys?Gly?Glu?Lys

145?????????????????150?????????????????155?????????????????160

Leu?Arg?Arg?Ile

 

<210>33

<211>513

<212>DNA

＜213〉rice

 

<220>

<221>CDS

<222>(1)..(513)

＜223〉contain the zinc finger protein matter (OS36821256) of A20 structural domain and AN1 structural domain

 

<400>33

atg?gcg?cag?agg?gag?aag?aag?gtg?gag?gag?ccg?acg?gag?ctg?agg?gcg?????48

Met?Ala?Gln?Arg?Glu?Lys?Lys?Val?Glu?Glu?Pro?Thr?Glu?Leu?Arg?Ala

1???????????????5???????????????????10??????????????????15

ccg?gag?atg?acg?ctc?tgc?gcc?aac?agc?tgc?ggg?ttc?ccg?ggc?aac?ccg?????96

Pro?Glu?Met?Thr?Leu?Cys?Ala?Asn?Ser?Cys?Gly?Phe?Pro?Gly?Asn?Pro

20??????????????????25??????????????????30

gcg?acc?aac?aac?ctc?tgc?cag?aac?tgc?ttc?ttg?gct?gcc?tcg?gcg?tct????144

Ala?Thr?Asn?Asn?Leu?Cys?Gln?Asn?Cys?Phe?Leu?Ala?Ala?Ser?Ala?Ser

35??????????????????40??????????????????45

tct?tct?tct?tct?tcc?gcc?gct?gcc?tcg?ccg?tcg?acg?acg?tcg?ttg?ccg????192

Ser?Ser?Ser?Ser?Ser?Ala?Ala?Ala?Ser?Pro?Ser?Thr?Thr?Ser?Leu?Pro

50??????????????????55??????????????????60

gtg?ttt?ccg?gtg?gtg?gag?aag?ccg?agg?cag?gcc?gta?cag?tcg?tcg?gcg????240

Val?Phe?Pro?Val?Val?Glu?Lys?Pro?Arg?Gln?Ala?Val?Gln?Ser?Ser?Ala

65??????????????????70??????????????????75??????????????????80

gcg?gcg?gcg?gtg?gcg?ctg?gtg?gtt?gag?cgg?ccg?acg?gcg?ggg?ccg?gtg????288

Ala?Ala?Ala?Val?Ala?Leu?Val?Val?Glu?Arg?Pro?Thr?Ala?Gly?Pro?Val

85??????????????????90??????????????????95

gag?tcg?tcg?tcg?aag?gcg?tcg?agg?tcg?tcg?tcg?gtc?aac?cga?tgc?cac????336

Glu?Ser?Ser?Ser?Lys?Ala?Ser?Arg?Ser?Ser?Ser?Val?Asn?Arg?Cys?His

100?????????????????105?????????????????110

agc?tgc?cgg?agg?cgg?gtg?ggc?ctg?acc?ggg?ttc?cgg?tgc?cgc?tgc?ggc????384

Ser?Cys?Arg?Arg?Arg?Val?Gly?Leu?Thr?Gly?Phe?Arg?Cys?Arg?Cys?Gly

115?????????????????120?????????????????125

gag?ctc?tac?tgc?ggc?gcg?cac?cgg?tac?tcc?gac?cgc?cac?gac?tgc?agc????432

Glu?Leu?Tyr?Cys?Gly?Ala?His?Arg?Tyr?Ser?Asp?Arg?His?Asp?Cys?Ser

130?????????????????135?????????????????140

ttc?gac?tac?aag?tcg?gcg?gcg?agg?gac?gcc?atc?gcc?agg?gag?aac?ccc????480

Phe?Asp?Tyr?Lys?Ser?Ala?Ala?Arg?Asp?Ala?Ile?Ala?Arg?Glu?Asn?Pro

145?????????????????150?????????????????155?????????????????160

gtc?gtc?cgc?gcc?gcc?aag?atc?gtt?agg?ttc?taa??????????????????????????513

Val?Val?Arg?Ala?Ala?Lys?Ile?Val?Arg?Phe

165?????????????????170

 

<210>34

<211>170

<212>PRT

＜213〉rice

 

<400>34

 

Met?Ala?Gln?Arg?Glu?Lys?Lys?Val?Glu?Glu?Pro?Thr?Glu?Leu?Arg?Ala

1???????????????5???????????????????10??????????????????15

Pro?Glu?Met?Thr?Leu?Cys?Ala?Asn?Ser?Cys?Gly?Phe?Pro?Gly?Asn?Pro

20??????????????????25??????????????????30

Ala?Thr?Asn?Asn?Leu?Cys?Gln?Asn?Cys?Phe?Leu?Ala?Ala?Ser?Ala?Ser

35??????????????????40??????????????????45

Ser?Ser?Ser?Ser?Ser?Ala?Ala?Ala?Ser?Pro?Ser?Thr?Thr?Ser?Leu?Pro

50??????????????????55??????????????????60

Val?Phe?Pro?Val?Val?Glu?Lys?Pro?Arg?Gln?Ala?Val?Gln?Ser?Ser?Ala

65??????????????????70??????????????????75??????????????????80

Ala?Ala?Ala?Val?Ala?Leu?Val?Val?Glu?Arg?Pro?Thr?Ala?Gly?Pro?Val

85??????????????????90??????????????????95

Glu?Ser?Ser?Ser?Lys?Ala?Ser?Arg?Ser?Ser?Ser?Val?Asn?Arg?Cys?His

100?????????????????105?????????????????110

Ser?Cys?Arg?Arg?Arg?Val?Gly?Leu?Thr?Gly?Phe?Arg?Cys?Arg?Cys?Gly

115?????????????????120?????????????????125

Glu?Leu?Tyr?Cys?Gly?Ala?His?Arg?Tyr?Ser?Asp?Arg?His?Asp?Cys?Ser

130?????????????????135?????????????????140

Phe?Asp?Tyr?Lys?Ser?Ala?Ala?Arg?Asp?Ala?Ile?Ala?Arg?Glu?Asn?Pro

145?????????????????150?????????????????155?????????????????160

Val?Val?Arg?Ala?Ala?Lys?Ile?Val?Arg?Phe

165?????????????????170

 

<210>35

<211>486

<212>DNA

＜213〉soybean

 

<220>

<221>CDS

<222>(1)..(486)

＜223〉contain the zinc finger protein matter (GM51659494) of A20 structural domain and AN1 structural domain

 

<400>35

atg?gct?cag?aaa?acc?gag?aas?gaa?gaa?acc?gac?ttc?aaa?gtt?ccg?gaa????48

Met?Ala?Gln?Lys?Thr?Glu?Lys?Glu?Glu?Thr?Asp?Phe?Lys?Val?Pro?Glu

1???????????????5???????????????????10??????????????????15

acg?att?acg?ctt?tgc?gtc?aac?aac?tgc?ggc?gtc?acc?gga?aac?cct?gcc????96

Thr?Ile?Thr?Leu?Cys?Val?Asn?Asn?Cys?Gly?Val?Thr?Gly?Asn?Pro?Ala

20??????????????????25??????????????????30

acg?aat?aac?atg?tgc?cag?aag?tgc?ttc?act?gcc?tct?acc?gcc?acc?act????144

Thr?Asn?Asn?Met?Cys?Gln?Lys?Cys?Phe?Thr?Ala?Ser?Thr?Ala?Thr?Thr

35??????????????????40??????????????????45

tcc?ggc?gcc?gga?ggt?gcc?gga?ata?gct?tct?ccg?gcg?acc?aga?tcc?ggc????192

Ser?Gly?Ala?Gly?Gly?Ala?Gly?Ile?Ala?Ser?Pro?Ala?Thr?Arg?Ser?Gly

50??????????????????55??????????????????60

gtc?tcc?gcg?cgt?cct?cag?aag?aga?tct?ttt?cct?gaa?gag?ccc?tcg?ccg????240

Val?Ser?Ala?Arg?Pro?Gln?Lys?Arg?Ser?Phe?Pro?Glu?Glu?Pro?Ser?Pro

65??????????????????70??????????????????75??????????????????80

gtg?gcg?gat?cct?cct?tct?tcg?gac?cag?acg?acg?ccg?tcg?gag?gcg?aag????288

Val?Ala?Asp?Pro?Pro?Ser?Ser?Asp?Gln?Thr?Thr?Pro?Ser?Glu?Ala?Lys

85??????????????????90??????????????????95

cgc?gtg?gtc?aac?cgc?tgc?tcc?gga?tgc?cgg?cgg?aag?gtc?gga?ctc?acc????336

Arg?Val?Val?Asn?Arg?Cys?Ser?Gly?Cys?Arg?Arg?Lys?Val?Gly?Leu?Thr

100?????????????????105?????????????????110

gga?ttc?cgg?tgc?cgg?tgc?ggc?gag?ctc?ttc?tgc?gcc?gag?cac?cgg?tac????384

Gly?Phe?Arg?Cys?Arg?Cys?Gly?Glu?Leu?Phe?Cys?Ala?Glu?His?Arg?Tyr

115?????????????????120?????????????????125

tcc?gac?cgc?cac?gac?tgc?agc?tat?gac?tac?aaa?gcc?gcc?gga?aga?gaa????432

Ser?Asp?Arg?His?Asp?Cys?Ser?Tyr?Asp?Tyr?Lys?Ala?Ala?Gly?Arg?Glu

130?????????????????135?????????????????140

gcc?atc?gcg?agg?gag?aat?ccg?gtg?atc?aga?gct?gcg?aag?atc?gtc?aaa????480

Ala?Ile?Ala?Arg?Glu?Asn?Pro?Val?Ile?Arg?Ala?Ala?Lys?Ile?Val?Lys

145?????????????????150?????????????????155?????????????????160

gtc?tga????????????????????????????????????????????????????????????486

Val

 

<210>36

<211>161

<212>PRT

＜213〉soybean

 

<400>36

 

Met?Ala?Gln?Lys?Thr?Glu?Lys?Glu?Glu?Thr?Asp?Phe?Lys?Val?Pro?Glu

1???????????????5???????????????????10??????????????????15

Thr?Ile?Thr?Leu?Cys?Val?Asn?Asn?Cys?Gly?Val?Thr?Gly?Asn?Pro?Ala

20??????????????????25??????????????????30

Thr?Asn?Asn?Met?Cys?Gln?Lys?Cys?Phe?Thr?Ala?Ser?Thr?Ala?Thr?Thr

35??????????????????40??????????????????45

Ser?Gly?Ala?Gly?Gly?Ala?Gly?Ile?Ala?Ser?Pro?Ala?Thr?Arg?Ser?Gly

50??????????????????55??????????????????60

Val?Ser?Ala?Arg?Pro?Gln?Lys?Arg?Ser?Phe?Pro?Glu?Glu?Pro?Ser?Pro

65??????????????????70??????????????????75??????????????????80

Val?Ala?Asp?Pro?Pro?Ser?Ser?Asp?Gln?Thr?Thr?Pro?Ser?Glu?Ala?Lys

85??????????????????90??????????????????95

Arg?Val?Val?Asn?Arg?Cys?Ser?Gly?Cys?Arg?Arg?Lys?Val?Gly?Leu?Thr

100?????????????????105?????????????????110

Gly?Phe?Arg?Cys?Arg?Cys?Gly?Glu?Leu?Phe?Cys?Ala?Glu?His?Arg?Tyr

115?????????????????120?????????????????125

Ser?Asp?Arg?His?Asp?Cys?Ser?Tyr?Asp?Tyr?Lys?Ala?Ala?Gly?Arg?Glu

130?????????????????135?????????????????140

Ala?Ile?Ala?Arg?Glu?Asn?Pro?Val?Ile?Arg?Ala?Ala?Lys?Ile?Val?Lys

145?????????????????150?????????????????155?????????????????160

Val

 

<210>37

<211>519

<212>DNA

＜213〉soybean

 

<220>

<221>CDS

<222>(1)..(519)

＜223〉contain the zinc finger protein matter (GM49780101) of A20 structural domain and AN1 structural domain

 

<400>37

atg?gag?cct?cat?gat?gag?act?gga?tgc?cag?gct?cct?gaa?cgc?ccc?att?????48

Met?Glu?Pro?His?Asp?Glu?Thr?Gly?Cys?Gln?Ala?Pro?Glu?Arg?Pro?Ile

1???????????????5???????????????????10??????????????????15

ctt?tgc?att?aat?aat?tgt?ggc?ttc?ttt?gga?aga?gca?gct?acc?atg?aac?????96

Leu?Cys?Ile?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Arg?Ala?Ala?Thr?Met?Asn

20??????????????????25??????????????????30

atg?tgt?tcc?aag?tgt?tac?aag?gac?atg?ctg?ttg?aag?cag?gag?cag?gac????144

Met?Cys?Ser?Lys?Cys?Tyr?Lys?Asp?Met?Leu?Leu?Lys?Gln?Glu?Gln?Asp

35??????????????????40??????????????????45

aaa?ttt?gca?gca?tca?tcc?gtt?gaa?aac?att?gtg?aat?ggc?agt?tcc?aat????192

Lys?Phe?Ala?Ala?Ser?Ser?Val?Glu?Asn?lle?Val?Asn?Gly?Ser?Ser?Asn

50??????????????????55??????????????????60

ggc?aat?gga?aag?cag?gct?gtg?gct?act?ggt?gct?gtt?gct?gta?caa?gtt????240

Gly?Asn?Gly?Lys?Gln?Ala?Val?Ala?Thr?Gly?Ala?Val?Ala?Val?Gln?Val

65??????????????????70??????????????????75??????????????????80

gaa?gct?gtg?gag?gtc?aag?att?gtc?tgt?gct?cag?agt?tct?gtg?gat?tcg????288

Glu?Ala?Val?Glu?Val?Lys?Ile?Val?Cys?Ala?Gln?Ser?Ser?Val?Asp?Ser

85??????????????????90??????????????????95

tcc?tcc?ggt?gat?agt?ttg?gag?atg?aaa?gcc?aag?act?ggt?ccc?agt?aga????336

Ser?Ser?Gly?Asp?Ser?Leu?Glu?Met?Lys?Ala?Lys?Thr?Gly?Pro?Ser?Arg

100?????????????????105?????????????????110

tgt?gct?aca?tgc?cgg?aaa?cgt?gtt?ggt?tta?act?ggt?ttc?agc?tgc?aaa????384

Cys?Ala?Thr?Cys?Arg?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Ser?Cys?Lys

115?????????????????120?????????????????125

tgt?ggc?aac?ctc?ttc?tgt?gca?atg?cat?cgc?tat?tct?gat?aaa?cat?gat????432

Cys?Gly?Asn?Leu?Phe?Cys?A1a?Met?His?Arg?Tyr?Ser?Asp?Lys?His?Asp

130?????????????????135?????????????????140

tgc?cct?ttt?gat?tat?agg?act?gtt?ggt?cag?gat?gcc?ata?gct?aaa?gcc????480

Cys?Pro?Phe?Asp?Tyr?Arg?Thr?Val?Gly?Gln?Asp?Ala?Ile?Ala?Lys?Ala

145?????????????????150?????????????????155?????????????????160

aac?ccc?ata?att?aag?gca?gat?aag?ctc?gac?aaa?atc?tag????????????????519

Asn?Pro?Ile?Ile?Lys?Ala?Asp?Lys?Leu?Asp?Lys?Ile

165?????????????????170

 

<210>38

<211>172

<212>PRT

＜213〉soybean

 

<400>38

 

Met?Glu?Pro?His?Asp?Glu?Thr?Gly?Cys?Gln?Ala?Pro?Glu?Arg?Pro?Ile

1???????????????5???????????????????10??????????????????15

Leu?Cys?Ile?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Arg?Ala?Ala?Thr?Met?Asn

20??????????????????25??????????????????30

Met?Cys?Ser?Lys?Cys?Tyr?Lys?Asp?Met?Leu?Leu?Lys?Gln?Glu?Gln?Asp

35??????????????????40??????????????????45

Lys?Phe?Ala?Ala?Ser?Ser?Val?Glu?Asn?Ile?Val?Asn?Gly?Ser?Ser?Asn

50??????????????????55??????????????????60

Gly?Asn?Gly?Lys?Gln?Ala?Val?Ala?Thr?Gly?Ala?Val?Ala?Val?Gln?Val

65??????????????????70??????????????????75??????????????????80

Glu?Ala?Val?Glu?Val?Lys?Ile?Val?Cys?Ala?Gln?Ser?Ser?Val?Asp?Ser

85??????????????????90??????????????????95

Ser?Ser?Gly?Asp?Ser?Leu?Glu?Met?Lys?Ala?Lys?Thr?Gly?Pro?Ser?Arg

100?????????????????105?????????????????110

Cys?Ala?Thr?Cys?Arg?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Ser?Cys?Lys

115?????????????????120?????????????????125

Cys?Gly?Asn?Leu?Phe?Cys?Ala?Met?His?Arg?Tyr?Ser?Asp?Lys?His?Asp

130?????????????????135?????????????????140

Cys?Pro?Phe?Asp?Tyr?Arg?Thr?Val?Gly?Gln?Asp?Ala?Ile?Ala?Lys?Ala

145?????????????????150?????????????????155?????????????????160

Asn?Pro?Ile?Ile?Lys?Ala?Asp?Lys?Leu?Asp?Lys?Ile

165?????????????????170

 

<210>39

<211>525

<212>DNA

＜213〉soybean

 

<220>

<221>CDS

<222>(1)..(525)

＜223〉contain the zinc finger protein matter (GM59637305) of A20 structural domain and AN1 structural domain

 

<400>39

atg?gac?cat?gac?aag?act?ggg?tgc?caa?gct?cct?cct?gaa?ggt?cct?ata?????48

Met?Asp?His?Asp?Lys?Thr?Gly?Cys?Gln?Ala?Pro?Pro?Glu?Gly?Pro?Ile

1???????????????5???????????????????10??????????????????15

ttg?tgc?atc?aac?aac?tgt?ggg?ttt?ttt?gga?agt?gca?gct?acc?atg?aac?????96

Leu?Cys?Ile?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Ala?Ala?Thr?Met?Asn

20??????????????????25??????????????????30

atg?tgt?tct?aaa?tgc?cac?aaa?gac?ata?ttg?ctg?aaa?cag?gag?cag?gcc????144

Met?Cys?Ser?Lys?Cys?His?Lys?Asp?Ile?Leu?Leu?Lys?Gln?Glu?Gln?Ala

35??????????????????40??????????????????45

aag?ctt?gca?gca?tca?tcc?att?ggg?aat?att?atg?aat?ggg?tca?tca?agc????192

Lys?Leu?Ala?Ala?Ser?Ser?Ile?Gly?Asn?Ile?Met?Asn?Gly?Ser?Ser?Ser

50??????????????????55??????????????????60

agc?act?gaa?aag?gaa?cct?gtt?gtt?gct?gct?gct?gct?aat?att?gat?atc????240

Ser?Thr?Glu?Lys?Glu?Pro?Val?Val?Ala?Ala?Ala?Ala?Asn?Ile?Asp?Ile

65??????????????????70??????????????????75??????????????????80

cca?gtt?att?cca?gta?gag?cct?aaa?act?gtc?tct?gtg?caa?cct?tta?ttt????288

Pro?Val?Ile?Pro?Val?Glu?Pro?Lys?Thr?Val?Ser?Val?Gln?Pro?Leu?Phe

85??????????????????90??????????????????95

ggt?tca?ggt?cca?gag?ggg?agt?gtt?gag?gca?aag?ccg?aag?gat?gga?cca????336

Gly?Ser?Gly?Pro?Glu?Gly?Ser?Val?Glu?Ala?Lys?Pro?Lys?Asp?Gly?Pro

100?????????????????105?????????????????110

aaa?cgt?tgc?agc?agc?tgc?aac?aag?cga?gtt?ggt?ttg?aca?ggg?ttt?aat????384

Lys?Arg?Cys?Ser?Ser?Cys?Asn?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Asn

115?????????????????120?????????????????125

tgt?cga?tgt?ggt?gac?ctt?ttt?ttg?tgc?tgt?aca?tcg?cta?ctc?gac?aag????432

Cys?Arg?Cys?Gly?Asp?Leu?Phe?Leu?Cys?Cys?Thr?Ser?Leu?Leu?Asp?Lys

130?????????????????135?????????????????140

cat?aat?tgc?cca?ttt?gat?tac?cgc?act?gcc?gct?caa?gat?gct?ata?gct????480

His?Asn?Cys?Pro?Phe?Asp?Tyr?Arg?Thr?Ala?Ala?Gln?Asp?Ala?Ile?Ala

145?????????????????150?????????????????155?????????????????160

aaa?gca?aac?cca?gtt?gtc?aag?gct?gaa?aag?ctt?gat?aag?atc?taa????????525

Lys?Ala?Asn?Pro?Val?Val?Lys?Ala?Glu?Lys?Leu?Asp?Lys?Ile

165?????????????????170

 

<210>40

<211>174

<212>PRT

＜213〉soybean

 

<400>40

 

Met?Asp?His?Asp?Lys?Thr?Gly?Cys?Gln?Ala?Pro?Pro?Glu?Gly?Pro?Ile

1???????????????5???????????????????10??????????????????15

Leu?Cys?Ile?Asn?Asn?Cys?Gly?Phe?Phe?Gly?Ser?Ala?Ala?Thr?Met?Asn

20??????????????????25??????????????????30

Met?Cys?Ser?Lys?Cys?His?Lys?Asp?Ile?Leu?Leu?Lys?Gln?Glu?Gln?Ala

35??????????????????40??????????????????45

Lys?Leu?Ala?Ala?Ser?Ser?Ile?Gly?Asn?Ile?Met?Asn?Gly?Ser?Ser?Ser

50??????????????????55??????????????????60

Ser?Thr?Glu?Lys?Glu?Pro?Val?Val?Ala?Ala?Ala?Ala?Asn?Ile?Asp?Ile

65??????????????????70??????????????????75??????????????????80

Pro?Val?Ile?Pro?Val?Glu?Pro?Lys?Thr?Val?Ser?Val?Gln?Pro?Leu?Phe

85??????????????????90??????????????????95

Gly?Ser?Gly?Pro?Glu?Gly?Ser?Val?Glu?Ala?Lys?Pro?Lys?Asp?Gly?Pro

100?????????????????105?????????????????110

Lys?Arg?Cys?Ser?Ser?Cys?Asn?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Asn

115?????????????????120?????????????????125

Cys?Arg?Cys?Gly?Asp?Leu?Phe?Leu?Cys?Cys?Thr?Ser?Leu?Leu?Asp?Lys

130?????????????????135?????????????????140

His?Asn?Cys?Pro?Phe?Asp?Tyr?Arg?Thr?Ala?Ala?Gln?Asp?Ala?Ile?Ala

145?????????????????150?????????????????155?????????????????160

Lys?Ala?Asn?Pro?Val?Val?Lys?Ala?Glu?Lys?Leu?Asp?Lys?Ile

165?????????????????170

 

<210>41

<211>498

<212>DNA

＜213〉wheat

 

<220>

<221>CDS

<222>(1)..(498)

＜223〉contain the zinc finger protein matter (TA55974113) of A20 structural domain and AN1 structural domain

 

<400>41

atg?gcg?cag?cgg?gat?cac?aag?cag?gag?gag?ccc?acg?gag?ctg?cgg?gcg????48

Met?Ala?Gln?Arg?Asp?His?Lys?Gln?Glu?Glu?Pro?Thr?Glu?Leu?Arg?Ala

1???????????????5???????????????????10??????????????????15

ccg?gag?atc?acg?ctc?tgc?gcc?aac?agc?tgc?ggc?ttc?ccg?ggc?aac?ccg?????96

Pro?Glu?Ile?Thr?Leu?Cys?Ala?Asn?Ser?Cys?Gly?Phe?Pro?Gly?Asn?Pro

20??????????????????25??????????????????30

gcc?acg?cag?aac?ctc?tgc?cag?aac?tgc?ttc?ttg?gcc?ggc?ccg?gcg?tcc????144

Ala?Thr?Gln?Asn?Leu?Cys?Gln?Asn?Cys?Phe?Leu?Ala?Gly?Pro?Ala?Ser

35??????????????????40??????????????????45

acg?tcg?ccg?tct?tcc?tcc?tcc?tcc?tcc?tcc?tct?tct?ctg?ccg?ggc?gtg????192

Thr?Ser?Pro?Ser?Ser?Ser?Ser?Ser?Ser?Ser?Ser?Ser?Leu?Pro?Gly?Val

50??????????????????55??????????????????60

tcc?gcg?ccg?acc?ccc?gtc?atc?gac?agg?ccg?agg?ccg?gcg?ccg?ttg?gag????240

Ser?Ala?Pro?Thr?Pro?Val?Ile?Asp?Arg?Pro?Arg?Pro?Ala?Pro?Leu?Glu

65??????????????????70??????????????????75??????????????????80

gcg?gag?ctg?gca?cgc?ccc?gcc?gtc?gac?ctt?gct?ccg?gcg?acg?gag?gcg????288

Ala?Glu?Leu?Ala?Arg?Pro?Ala?Val?Asp?Leu?Ala?Pro?Ala?Thr?Glu?Ala

85??????????????????90??????????????????95

aag?ccg?gcg?agg?acg?tcg?gtg?aac?cgg?tgc?tcc?agc?tgc?cgg?aag?cgc????336

Lys?Pro?Ala?Arg?Thr?Ser?Val?Asn?Arg?Cys?Ser?Ser?Cys?Arg?Lys?Arg

100?????????????????105?????????????????110

gtg?ggg?ctg?acg?ggg?ttc?cgg?tgc?cgg?tgc?ggc?gac?atg?ttc?tgc?ggc????384

Val?Gly?Leu?Thr?Gly?Phe?Arg?Cys?Arg?Cys?Gly?Asp?Met?Phe?Cys?Gly

115?????????????????120?????????????????125

gag?cac?cgg?tac?tcg?gac?cgg?cac?ggg?tgc?agc?tac?gac?tac?aag?gcc????432

Glu?His?Arg?Tyr?Ser?Asp?Arg?His?Gly?Cys?Ser?Tyr?Asp?Tyr?Lys?Ala

130?????????????????135?????????????????140

gcc?gcc?agg?gac?gcc?atc?gcc?agg?gac?aac?ccc?gtc?gtg?cgc?gcc?gcc????480

Ala?Ala?Arg?Asp?Ala?Ile?Ala?Arg?Asp?Asn?Pro?Val?Val?Arg?Ala?Ala

145?????????????????150?????????????????155?????????????????160

aag?atc?gtc?agg?ttc?tga????????????????????????????????????????????498

Lys?Ile?Val?Arg?Phe

165

 

<210>42

<211>165

<212>PRT

＜213〉wheat

 

<400>42

 

Met?Ala?Gln?Arg?Asp?His?Lys?Gln?Glu?Glu?Pro?Thr?Glu?Leu?Arg?Ala

1???????????????5???????????????????10??????????????????15

Pro?Glu?Ile?Thr?Leu?Cys?Ala?Asn?Ser?Cys?Gly?Phe?Pro?Gly?Asn?Pro

20??????????????????25??????????????????30

Ala?Thr?Gln?Asn?Leu?Cys?Gln?Asn?Cys?Phe?Leu?Ala?Gly?Pro?Ala?Ser

35??????????????????40??????????????????45

Thr?Ser?Pro?Ser?Ser?Ser?Ser?Ser?Ser?Ser?Ser?Ser?Leu?Pro?Gly?Val

50??????????????????55??????????????????60

Ser?Ala?Pro?Thr?Pro?Val?Ile?Asp?Arg?Pro?Arg?Pro?Ala?Pro?Leu?Glu

65??????????????????70??????????????????75??????????????????80

Ala?Glu?Leu?Ala?Arg?Pro?Ala?Val?Asp?Leu?Ala?Pro?Ala?Thr?Glu?Ala

85??????????????????90??????????????????95

Lys?Pro?Ala?Arg?Thr?Ser?Val?Asn?Arg?Cys?Ser?Ser?Cys?Arg?Lys?Arg

100?????????????????105?????????????????110

Val?Gly?Leu?Thr?Gly?Phe?Arg?Cys?Arg?Cys?Gly?Asp?Met?Phe?Cys?Gly

115?????????????????120?????????????????125

Glu?His?Arg?Tyr?Ser?Asp?Arg?His?Gly?Cys?Ser?Tyr?Asp?Tyr?Lys?Ala

130?????????????????135?????????????????140

Ala?Ala?Arg?Asp?Ala?Ile?Ala?Arg?Asp?Asn?Pro?Val?Val?Arg?Ala?Ala

145?????????????????150?????????????????155?????????????????160

Lys?Ile?Val?Arg?Phe

165

 

<210>43

<211>207

<212>PRT

＜213〉small liwan moss

<220>

<221>MISC_FEATURE

<222>(1)..(207)

＜223〉methionine sulfoxide reductase family protein (EST65)

 

<400>43

 

Met?Val?Ala?Glu?Ser?Val?Leu?Val?Cys?Arg?Ser?Ser?Val?Val?Gly?Ala

1???????????????5???????????????????10??????????????????15

Gly?Leu?Gln?Ser?Phe?Val?Gly?Glu?Gly?Ala?Lys?Arg?Glu?Ser?Ala?Gly

20??????????????????25??????????????????30

Pro?Gly?Arg?Ser?Val?Phe?Leu?GlyAla?Gln?Val?Gln?Lys?Met?Gly?Ala

35??????????????????40??????????????????45

Gly?Met?Ser?Ala?Arg?Ser?Asp?Val?Arg?Pro?Ala?Ala?Val?Pro?Lys?Ala

50??????????????????55??????????????????60

Ser?Gly?Asp?Val?Ser?Glu?Gln?Thr?Asp?Tyr?Lys?Thr?Phe?Ser?Asp?Glu

65??????????????????70??????????????????75??????????????????80

Glu?Trp?Lys?Lys?Arg?Leu?Ser?Gln?Gln?Gln?Phe?Tyr?Val?Ala?Arg?Lys

85??????????????????90??????????????????95

Lys?Gly?Thr?Glu?Arg?Pro?Phe?Thr?Gly?Glu?Tyr?Trp?Asn?Thr?Lys?Thr

100?????????????????105?????????????????110

Ala?Gly?Thr?Tyr?Leu?Cys?Val?Cys?Cys?Lys?Thr?Pro?Leu?Phe?Ser?Ser

115?????????????????120?????????????????125

Lys?Thr?Lys?Phe?Asp?Ser?Gly?Thr?Gly?Trp?Pro?Ser?Tyr?Tyr?Asp?Thr

130?????????????????135?????????????????140

Ile?Gly?Asp?Asn?Val?Lys?Ser?His?Met?Asp?Trp?Ser?Ile?Pro?Phe?Met

145?????????????????150?????????????????155?????????????????160

Pro?Arg?Thr?Glu?Val?Val?Cys?Ala?Val?Cys?Asp?Ala?His?Leu?Gly?His

165?????????????????170?????????????????175

Val?Phe?Asp?Asp?Gly?Pro?Arg?Pro?Thr?Gly?Lys?Arg?Tyr?Cys?Ile?Asn

180?????????????????185?????????????????190

Ser?Ala?Ala?Ile?Asp?Leu?Lys?Ala?Glu?Lys?Gln?Glu?Glu?Arg?Asn

195?????????????????200?????????????????205

 

<210>44

<211>339

<212>PRT

＜213〉small liwan moss

 

<220>

<221>MISC_FEATURE

<222>(1)..(339)

＜223〉homeodomain-leucine zipper protein matter (EST12)

 

<400>44

 

Met?Val?Val?Pro?Ser?Leu?Pro?Ala?Phe?Gly?Gly?Gln?Asn?Ala?Met?Leu

1???????????????5???????????????????10??????????????????15

Arg?Arg?Asn?Ile?Asp?Asn?Asn?Thr?Asp?Thr?Leu?Ile?Ser?Leu?Leu?Gln

20??????????????????25??????????????????30

Gly?Ser?Cys?Ser?Pro?Arg?Val?Ser?Met?Gln?Gln?Val?Pro?Arg?Ser?Ser

35??????????????????40??????????????????45

Glu?Ser?Leu?Glu?Asn?Met?Met?Gly?Ala?Cys?Gly?Gln?Lys?Leu?Pro?Tyr

50??????????????????55??????????????????60

Phe?Ser?Ser?Phe?Asp?Gly?Pro?Ser?Val?Glu?Glu?Gln?Glu?Asp?Val?Asp

65??????????????????70??????????????????75??????????????????80

Glu?Gly?Ile?Asp?Glu?Phe?Ala?His?His?Val?Glu?Lys?Lys?Arg?Arg?Leu

85??????????????????90??????????????????95

Ser?Leu?Glu?Gln?Val?Arg?Ser?Leu?Glu?Arg?Asn?Phe?Glu?Val?Glu?Asn

100?????????????????105?????????????????110

Lys?Leu?Glu?Pro?Glu?Arg?Lys?Met?Gln?Leu?Ala?Lys?Glu?Leu?Gly?Leu

115?????????????????120?????????????????125

Arg?Pro?Arg?Gln?Val?Ala?Val?Trp?Phe?Gln?Asn?Arg?Arg?Ala?Arg?Trp

130?????????????????135?????????????????140

Lys?Thr?Lys?Gln?Leu?Glu?His?Asp?Tyr?Glu?Thr?Leu?Lys?Lys?Ala?Tyr

145?????????????????150?????????????????155?????????????????160

Asp?Arg?Leu?Lys?Ala?Asp?Phe?Glu?Ala?Val?Thr?Leu?Asp?Thr?Asn?Ala

165?????????????????170?????????????????175

Leu?Lys?Ala?Glu?Val?Ser?Arg?Leu?Lys?Gly?Ile?Ser?Asn?Asp?Asp?Val

180?????????????????185?????????????????190

Lys?Pro?Ala?Glu?Phe?Val?Gln?Gly?Lys?Cys?Asp?Thr?Thr?Ser?His?Pro

195?????????????????200?????????????????205

Ala?Ser?Pro?Ala?Gln?Ser?Glu?Arg?Ser?Asp?Ile?Val?Ser?Ser?Arg?Asn

210?????????????????215?????????????????220

Arg?Thr?Thr?Pro?Thr?Ile?His?Val?Asp?Pro?Val?Ala?Pro?Glu?Glu?Ala

225?????????????????230?????????????????235?????????????????240

Gly?Ala?His?Leu?Thr?Met?Ser?Ser?Asp?Ser?Asn?Ser?Ser?Glu?Val?Met

245?????????????????250?????????????????255

Asp?Ala?Asp?Ser?Pro?Arg?Thr?Ser?His?Thr?Ser?Ala?Ser?Arg?Ser?Thr

260?????????????????265?????????????????270

Leu?Ser?Thr?Ser?Val?Val?Gln?Pro?Asp?Glu?Gly?Leu?Gly?Val?Ala?Gln

275?????????????????280?????????????????285

Tyr?Pro?His?Phe?Ser?Pro?Glu?Asn?Phe?Val?Gly?Pro?Asn?Met?Pro?Glu

290?????????????????295?????????????????300

Ile?Cys?Ala?Asp?Gln?Ser?Leu?Ala?Ser?Gln?Val?Lys?Leu?Glu?Glu?Ile

305?????????????????310?????????????????315?????????????????320

His?Ser?Phe?Asn?Pro?Asp?Gln?Thr?Phe?Leu?Leu?Leu?Pro?Asn?Trp?Trp

325?????????????????330?????????????????335

Asp?Trp?Ala

 

<210>45

<211>188

<212>PRT

＜213〉small liwan moss

 

<220>

<221>MISC_FEATURE

<222>(1)..(188)

＜223〉contain the zinc finger protein matter (EST307) of A20 structural domain and AN1 structural domain

 

<400>45

 

Met?Ala?Thr?Glu?Arg?Val?Ser?Gln?Glu?Thr?Thr?Ser?Gln?Ala?Pro?Glu

1???????????????5???????????????????10??????????????????15

Gly?Pro?Val?Met?Cys?Lys?Asn?Leu?Cys?Gly?Phe?Phe?Gly?Ser?Gln?Ala

20??????????????????25??????????????????30

Thr?Met?Gly?Leu?Cys?Ser?Lys?Cys?Tyr?Arg?Glu?Thr?Val?Met?Gln?Ala

35??????????????????40??????????????????45

Lys?Met?Thr?Ala?Leu?Ala?Glu?Gln?Ala?Thr?Gln?Ala?Ala?Gln?Ala?Thr

50??????????????????55??????????????????60

Ser?Ala?Thr?Ala?Ala?Ala?Val?Gln?Pro?Pro?Ala?Pro?Val?His?Glu?Thr

65??????????????????70??????????????????75??????????????????80

Lys?Leu?Thr?Cys?Glu?Val?Glu?Arg?Thr?Met?Ile?Val?Pro?His?Gln?Ser

85??????????????????90??????????????????95

Ser?Ser?Tyr?Gln?Gln?Asp?Leu?Val?Thr?Pro?Ala?Ala?Ala?Ala?Pro?Gln

100?????????????????105?????????????????110

Ala?Val?Lys?Ser?Ser?Ile?Ala?Ala?Pro?Ser?Arg?Pro?Glu?Pro?Asn?Arg

115?????????????????120?????????????????125

Cys?Gly?Ser?Cys?Arg?Lys?Arg?Val?Gly?Leu?Thr?Gly?Phe?Lys?Cys?Arg

130?????????????????135?????????????????140

Cys?Gly?Asn?Leu?Tyr?Cys?Ala?Leu?His?Arg?Tyr?Ser?Asp?Lys?His?Thr

145?????????????????150?????????????????155?????????????????160

Cys?Thr?Tyr?Asp?Tyr?Lys?Ala?Ala?Gly?Gln?Glu?Ala?Ile?Ala?Lys?Ala

165?????????????????170?????????????????175

Asn?Pro?Leu?Val?Val?Ala?Glu?Lys?Val?Val?Lys?Phe

180?????????????????185

Claims (7)

1. with the transfer-gen plant of expression cassette conversion, described expression cassette comprises isolating polynucleotide, and the sequence of its encoded polypeptides is selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQID NO:8 and SEQ ID NO:10.

2. with the transfer-gen plant of expression cassette conversion, described expression cassette comprises isolating polynucleotide, and the sequence of its encoded polypeptides is selected from SEQ ID NO:12, SEQ ID NO:14 and SEQ ID NO:16.

3. with the transfer-gen plant of expression cassette conversion, described expression cassette comprises isolating polynucleotide, and the sequence of its encoded polypeptides is selected from SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42 and SEQ ID NO:44.

4. isolating polynucleotide, its sequence are selected from the listed polymerized nucleoside acid sequence of table 1.

5. isolated polypeptide, its sequence is selected from the listed peptide sequence of table 1.

6. produce the method for the transfer-gen plant that comprises the listed at least a polynucleotide of table 1; wherein the expression of polynucleotide in plant caused plant to be compared with the multiple plant of wild-type; under normal or condition that water is limited, growth and/or output have been increased; and/or having increased tolerance to environment-stress, described method comprises the following steps:

(a) will comprise the expression vector introduced plant cell of the listed at least a polynucleotide of table 1,

(b) produce the transfer-gen plant of expressing polynucleotide by this vegetable cell; wherein the expression of polynucleotide in transfer-gen plant makes plant compare with the multiple plant of wild-type; under normal or condition that water is limited, increase growth and/or output, and/or increase tolerance environment-stress.

7. increase plant under normal or condition that water is limited growth and/or output and/or increase the method for plant to the tolerance of environment-stress, described method is included in the step of the expression of the listed at least a polynucleotide of increase table 1 in the plant.

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