CN101411375B - Cold tea for preventing and treating coprostasis type sub-health and preparation method thereof - Google Patents

Abstract

Translated fromChinese

本发明提供了一种防治亚健康便秘的凉茶，该凉茶由水和以下重量配比的原料制备得到：生何首乌15～40份、蒲公英15～40份、枳实5～25份、决明子5～20份和白糖10～30份。本发明凉茶具有润肠通便的作用，可以很好的缓解亚健康人群的便秘症状；另外，本发明所述凉茶药性温和，味甘凉而不苦，易为人们接受；所用的原料药均为常见药材，价格适中，材料易得，且制备工艺简单，适于产业化生产。The invention provides a herbal tea for preventing and treating sub-healthy constipation. The herbal tea is prepared from water and the following raw materials in weight ratio: 15-40 parts of raw Polygonum multiflorum, 15-40 parts of dandelion, 5-25 parts of Citrus aurantium, and 5-40 parts of cassia seed 20 parts and 10-30 parts of white sugar. The herbal tea of the present invention has the function of moistening the intestines and laxatives, and can well alleviate the constipation symptoms of sub-healthy people; in addition, the herbal tea of the present invention is mild in nature, sweet and cool in taste but not bitter, and is easily accepted by people; the raw materials used are all It is a common medicinal material, the price is moderate, the material is easy to obtain, and the preparation process is simple, so it is suitable for industrial production.

Description

Translated fromChinese

一种防治便秘型亚健康的凉茶及其制备方法A kind of herbal tea for preventing and treating constipation type sub-health and preparation method thereof

技术领域technical field

本发明涉及医药领域，具体涉及医用配制品。The invention relates to the field of medicine, in particular to medical preparations.

背景技术Background technique

亚健康状态(Sub-health)是健康与疾病之间的临界状态，是机体在无器质性病变情况下发生了一些功能性病变，其临床表现多种多样，但临床检查无异常，且不能确诊为疾病。。据统计，在我国有60～70％的人群处于亚健康状态，已是当今危害人们健康的头号隐形杀手。但是亚健康状态的发生机制尚未完全明确，现代医学对其亦无较好的防治办法。Sub-health state (Sub-health) is a critical state between health and disease, where some functional lesions occur in the body without organic lesions. Diagnosed with disease. . According to statistics, 60-70% of the population in our country is in a sub-health state, which is the number one invisible killer that endangers people's health today. But the occurrence mechanism of the sub-health state is not completely clear, and modern medicine has no good prevention and treatment method for it.

前期研究发现，亚健康状态可分为疲劳、失眠、咽喉不爽、便秘、眼睛干涩等为主的五个症候群，即亚健康状态的五个亚型。中医学整体观念与辨证论治的特点决定了祖国医学防治亚健康的优势明显。2006年1月国家中医药管理局在北京举行了“世界中医药学会联合会亚健康专业委员会成立暨首届世界亚健康学术大会”，会议上制订了《亚健康的中医临床研究指导原则(试行)》，提示我国亚健康的中医研究进入了空前活跃的阶段。本课题组对广东地区人群亚健康状态进行了临床流行病学调查研究，结果显示：约有60％的人群处于亚健康状态。面对如此庞大的亚健康人群，尽管中医药对防治亚健康有明显的疗效，但亚健康的知晓率并不高，能主动就诊者则更少，中药汤剂服用不便，限制了中医药防治亚健康优势的发挥。因此，寻找一种简便有效的中医药方法防治亚健康势在必行。Preliminary studies have found that the sub-health state can be divided into five syndromes, namely fatigue, insomnia, sore throat, constipation, and dry eyes, which are the five sub-types of the sub-health state. The overall concept of traditional Chinese medicine and the characteristics of syndrome differentiation and treatment determine that the motherland's medicine has obvious advantages in the prevention and treatment of sub-health. In January 2006, the State Administration of Traditional Chinese Medicine held the "Establishment of the Sub-health Professional Committee of the World Federation of Chinese Medicine Societies and the First World Academic Conference on Sub-health" in Beijing. At the meeting, the "Guidelines for Clinical Research of Sub-health in Traditional Chinese Medicine (Trial)" was formulated. ", suggesting that Chinese medicine research on sub-health in my country has entered an unprecedentedly active stage. Our research group conducted a clinical epidemiological investigation on the sub-health state of the population in Guangdong area, and the results showed that about 60% of the population was in a sub-health state. Faced with such a large sub-health population, although traditional Chinese medicine has obvious effects on the prevention and treatment of sub-health, the awareness rate of sub-health is not high, and even fewer people can actively seek medical treatment. The inconvenience of taking traditional Chinese medicine decoction limits the prevention and treatment of traditional Chinese medicine. The advantage of sub-health is brought into play. Therefore, it is imperative to find a simple and effective traditional Chinese medicine method to prevent and treat sub-health.

国知局2005年9月7日授权公告了一项发明专利“一种调治亚健康的中药制剂及其制备方法”(授权公告号CN1217685)，该专利公开了一种由以下重量份原料制得的中药制剂：人参10-50、刺吾50-250、龟板40-80、灵芝80-160、五味子10-50、龙骨40-80、远志40-80、茯苓60-140、合欢花40-80、石菖蒲10-50和蔓荆子40-80，该制剂具有增强机体活力、提高反应能力和适应能力、改善记忆力、抗疲劳等多种提高体力脑力的功效，可起到调治人体亚健康状态的作用。但尚未有改善便秘型亚健康的药物及保健食品。On September 7, 2005, the State Intellectual Property Bureau authorized and announced an invention patent "a traditional Chinese medicine preparation for regulating sub-health and its preparation method" (authorized announcement number CN1217685). Traditional Chinese medicine preparations: ginseng 10-50, thorn 50-250, tortoise shell 40-80, ganoderma lucidum 80-160, schisandra 10-50, keel 40-80, polygala 40-80, poria cocos 60-140, albizia 40-80 , Shichangpu 10-50 and Vitex 40-80, the preparation has the effects of enhancing body vitality, improving response ability and adaptability, improving memory, anti-fatigue, etc. role. But there is no medicine and health food for improving constipation-type sub-health.

便秘是亚健康较为常见的一种症状，我们对广东地区亚健康部分人群(3981例)的调查，有便秘症状者有1386例，在亚健康人群中的发生率为34.8％。虽然用于治疗便秘的药物不少，但未见有专门针对亚健康便秘这一症状的保健凉茶相关产品和报道。Constipation is a relatively common symptom of sub-health. We investigated some sub-health populations (3981 cases) in Guangdong area, 1386 cases had constipation symptoms, and the incidence rate in sub-health population was 34.8%. Although there are many medicines for treating constipation, there are no health-care herbal tea-related products and reports specifically aimed at the symptom of sub-healthy constipation.

发明内容Contents of the invention

本发明要解决的技术问题是提供一种防治亚健康便秘的凉茶。The technical problem to be solved by the present invention is to provide a herbal tea for preventing and treating sub-healthy constipation.

本发明解决上述技术问题的技术方案是：The technical scheme that the present invention solves the problems of the technologies described above is:

一种防治亚健康便秘的凉茶，该凉茶由水和以下重量配比的原料制备得到：生何首乌15～40份、蒲公英15～40份、枳实5～25份、决明子5～20份和白糖10～30份。A herbal tea for preventing and treating sub-healthy constipation. The herbal tea is prepared from water and the following raw materials in weight ratio: 15-40 parts of raw Polygonum multiflorum, 15-40 parts of dandelion, 5-25 parts of Citrus aurantium, 5-20 parts of cassia seed and white sugar 10 to 30 servings.

上述方案中，所述的原料配比优选生何首乌20～35份、蒲公英20～35份、枳实10～20份、决明子10～20份和白糖10～30份，最佳为生何首乌20份、蒲公英20份、枳实15份、决明子15份和白糖15份。In the above scheme, the ratio of raw materials is preferably 20-35 parts of raw Polygonum multiflorum, 20-35 parts of Dandelion, 10-20 parts of Citrus aurantium, 10-20 parts of Cassia seed and 10-30 parts of sugar, and the best is 20 parts of Raw Polygonum multiflorum , 20 parts of dandelion, 15 parts of citrus aurantium, 15 parts of cassia seed and 15 parts of white sugar.

本发明所述凉茶的制备方法由以下步骤组成：The preparation method of cold tea of the present invention is made up of the following steps:

(1)将所述原料按分别以下方法处理：(1) The raw materials are processed by the following methods respectively:

将白糖经过高温杀菌后，加入8～12倍水，通过蒸汽微热并充分搅拌溶解后，过滤，得白糖水；After the white sugar is sterilized at high temperature, add 8 to 12 times of water, heat slightly with steam and fully stir to dissolve, then filter to obtain white sugar water;

将何首乌、蒲公英、枳实、决明子经过浸泡30分钟后，放入原料罐中，加入8～12倍水，煮沸2小时，抽出液体，再次在原料罐中加入8～12倍水，煮沸2小时，合并两次煎煮液，过滤；After soaking Polygonum multiflorum, Dandelion, Citrus aurantium and Cassia seeds for 30 minutes, put them into the raw material tank, add 8 to 12 times of water, boil for 2 hours, take out the liquid, add 8 to 12 times of water in the raw material tank again, and boil for 2 hours , combine two decoctions, and filter;

(2)配制药液：将经上述白糖水加入到所述的煎煮液中，进行充分搅拌，得药液；(2) Prepare medicinal liquid: add the above-mentioned white sugar water into the described decoction liquid, fully stir to obtain medicinal liquid;

(3)采用ZTC1+1 II型天然澄清剂澄清过滤：按ZTC1+1 II型天然澄清剂的产品说明书配制好所述澄清剂中的A组分溶液和B组分溶液，然后将6％药液体积的B组分溶液缓慢细流加入药液中，搅匀，再将8％药液体积的A组分缓慢细流加入药液中，搅匀，置60～70℃水浴中保温1h，然后常温静置24h，粗滤后离心，取上清夜，加水至所用原药的质量和药液的体积比为1∶30～60。(3) Use ZTC1+1 type II natural clarifier for clarification and filtration: prepare component A solution and component B solution in the clarifier according to the product manual of ZTC1+1 type II natural clarifier, and then add 6% of the medicine Slowly add the liquid volume of component B solution into the medicinal liquid in a trickle, stir well, then slowly add 8% of the medicinal liquid volume of component A into the medicinal liquid in a trickle, stir well, and keep it in a water bath at 60-70°C for 1 hour. Then stand at room temperature for 24 hours, centrifuge after coarse filtration, take the supernatant, add water until the mass of the original drug used and the volume ratio of the drug solution are 1:30-60.

(4)杀菌冷却：采用蒸汽进行杀菌，杀菌温度为120℃，保持30分钟，反压冷却至30～40℃；(4) Sterilization and cooling: use steam to sterilize, the sterilization temperature is 120°C, keep for 30 minutes, and cool to 30-40°C under back pressure;

(5)灌装封口：在真空度为0.03～0.04Mpa，药液温度为60～65℃的条件下真空灌装，罐装后及时封口。(5) Filling and sealing: Vacuum filling under the conditions of a vacuum degree of 0.03-0.04Mpa and a liquid medicine temperature of 60-65°C, and sealing in time after filling.

本发明所述方法中各步骤严格受到无菌控制，步骤(1)中所用的水应符合直饮水的标准。Each step in the method of the present invention is strictly subject to aseptic control, and the water used in step (1) should meet the standard of direct drinking water.

本发明方法中所用的ZTC1+1 II型天然澄清剂是一种食品添加剂，由A组分和B组分组成，其中A组分可在不同的可溶性大分子之间“架桥”连接使分子迅速增大，B组分在A组分所形成的复合物的基础上再“架桥”，使絮状物尽快形成沉淀以便除去。ZTC1+1 II型天然澄清剂使用前应按说明书配制成溶液，具体配制方法如下：The ZTC1+1 type II natural clarifier used in the method of the present invention is a kind of food additive, is made up of A component and B component, and wherein A component can " bridging " link between different soluble macromolecules to make molecule Rapidly increasing, the B component will "bridge" on the basis of the complex formed by the A component, so that the floc can form a precipitate as soon as possible for removal. ZTC1+1 Type II natural clarifier should be prepared into a solution according to the instructions before use. The specific preparation method is as follows:

A组分溶液：称取1Kg A组分细粉，加少量水，搅拌成糊状，再加水定容至100L，静置24h，搅拌，双层纱布过滤。Component A solution: Weigh 1Kg component A fine powder, add a small amount of water, stir to form a paste, add water to make up to 100L, let stand for 24 hours, stir, and filter with double gauze.

B组分溶液：称取1Kg B组分细粉，加少量1％冰醋酸，搅拌成糊状，再加1％冰醋酸定容至100L，静置24h，搅拌，双层纱布过滤。Component B solution: Weigh 1Kg component B fine powder, add a small amount of 1% glacial acetic acid, stir to form a paste, add 1% glacial acetic acid to make up to 100L, let stand for 24 hours, stir, and filter with double gauze.

本发明选用生何首乌、蒲公英、枳实、决明子等天然原料来配置，不含任何化学成分的防腐剂、色素、香精及添加剂，能够润肠通便、滋益肝肾，解毒通腹、通补结合，可以明显的改善亚健康人群的便秘情况。其中，The present invention uses natural raw materials such as Polygonum multiflorum, Dandelion, Citrus aurantium, Semen Cassiae and other natural materials to prepare, does not contain any preservatives, pigments, flavors and additives of chemical components, can moisten the intestines and laxatives, nourish the liver and kidney, detoxify the abdomen, and relieve tonic. Combined, it can significantly improve the constipation of sub-healthy people. in,

生何首乌具有通便解毒截疟、补益精血，固肾乌发的作用，可治肠燥便秘、肝肾精血亏虚之头晕目眩、心悸失眠、萎黄乏力、须发早白；Radix Polygoni Multiflori has the effects of laxative, detoxifying, intercepting malaria, nourishing essence and blood, strengthening kidney and black hair, and can cure dryness of the intestines and constipation, dizziness, palpitation, insomnia, chlorosis and fatigue, premature graying of beard and hair due to deficiency of liver and kidney essence and blood;

蒲公英具有清热解毒和利尿散结的作用，在本发明凉茶中可以起到清肠排毒之功。Dandelion has the effects of clearing away heat and detoxification, diuresis and resolving stagnation, and can play the merit of clearing intestines and detoxifying in the herbal tea of the present invention.

枳实具有破气除痞，化痰消积，利五脏和益气轻身的作用；Citrus aurantium has the functions of dispelling qi and eliminating swelling, resolving phlegm and eliminating stagnation, benefiting the five internal organs, nourishing qi and lightening the body;

决明子有清肝、明目、利水、通便之功，治风热赤眼，青盲，雀目，高血压，肝炎，肝硬化腹水和习惯性便秘；Cassia seed has the functions of clearing the liver, improving eyesight, diuresis, and laxative, and can cure red eyes due to wind-heat, blue blindness, bird eyes, high blood pressure, hepatitis, liver cirrhosis, ascites, and habitual constipation;

白糖具有润肺生津的作用；White sugar has the effect of nourishing the lungs and promoting body fluid;

以上诸药合用可以通补兼施、润肠通便，很好的缓解亚健康人群的便秘症状；另外，本发明凉茶药性温和，味甘凉而不苦，易为人们接受。本发明凉茶所用的原料药均为常见药材，价格适中，材料易得，且制备工艺简单，适于产业化生产。The combined use of the above-mentioned medicines can both tonify and relieve constipation, relieve constipation symptoms of sub-healthy people; in addition, the herbal tea of the present invention has mild medicinal properties, sweet and cool taste but not bitter, and is easily accepted by people. The crude drugs used in the herbal tea of the invention are all common medicinal materials, the price is moderate, the materials are easy to obtain, and the preparation process is simple, which is suitable for industrial production.

为了更好理解本发明，现通过实验研究本发明凉茶对实验小鼠的小肠运动、首次排便时间、粪便粒数和粪便重量的影响，以探讨其通便功能作用。In order to better understand the present invention, the effect of herbal tea of the present invention on the small intestine movement, first defecation time, number of feces and weight of feces of experimental mice is studied by experiment to explore its laxative function.

1 材料与方法1 Materials and methods

1.1 受试物及供试液制备1.1 Preparation of test substance and test solution

受试物为实施例1所制备的防止便秘型亚健康凉茶，人体推荐量(按所含原药重量)为120～240mg/Kg.BW.d。The test substance is the anti-constipation type sub-healthy herbal tea prepared in Example 1, and the recommended amount for human body (according to the weight of the original drug contained) is 120-240 mg/Kg.BW.d.

1.2 实验动物1.2 Experimental animals

SPF级昆明种雄性小鼠120只，体重为18～22g，由南方医科大学医学实验动物中心提供。实验动物房合格证号：SCXK粤2006-0015。120 SPF-grade Kunming male mice, weighing 18-22 g, were provided by the Medical Experimental Animal Center of Southern Medical University. Laboratory animal room qualification certificate number: SCXK Guangdong 2006-0015.

1.3 实验分组：本发明凉茶给药设低、中、高三个剂量组，剂量分别是1200、2400、3600mg/kg.BW(分别相当于人体最小推荐量的10、20、30倍)，同时设便秘模型对照组和阴性对照组。每组12只动物。1.3 Experimental grouping: the herbal tea administration of the present invention is established in three dose groups of low, medium and high, and the doses are respectively 1200, 2400, and 3600 mg/kg.BW (respectively equivalent to 10, 20, and 30 times the minimum recommended dose for the human body). Constipation model control group and negative control group. 12 animals per group.

1.4 仪器与试剂手术剪、眼科镊、直尺、电子天平、活性炭粉、阿拉伯树胶(上海化学试剂站分装厂，批号：19950314)、复方地芬诺酯片(江苏常州武进制药厂生产，批号：20010301)等。1.4 Instruments and reagents Surgical scissors, ophthalmic tweezers, ruler, electronic balance, activated carbon powder, gum arabic (Shanghai Chemical Reagent Station Subpackaging Factory, batch number: 19950314), compound diphenoxylate tablets (produced by Jiangsu Changzhou Wujin Pharmaceutical Factory, batch number : 20010301), etc.

1.5 实验方法1.5 Experimental method

1.5.1 小肠运动实验1.5.1 Small intestine motility test

各实验组按1200、2400、3600mg/kg.BW(均调至相同体积)经口给予本发明凉茶，两个对照组给予等体积的蒸馏水，每天1次，连续给本发明凉茶7天。末次给样的当天晚上各组动物禁食(饮水不限)，16小时后模型对照组和3个剂量组给予剂量为2mg/kg.B W的复方地芬诺酯溶液(0.2m1/10g.BW)，阴性对照组给予蒸馏水。30分钟后，3个剂量组给予含本发明凉茶的墨汁(含5％的活性炭粉、10％阿拉伯树胶)，模型对照组和阴性对照组给予墨汁。给予墨汁25分钟后颈椎脱臼处死动物，打开腹腔分离肠系膜，剪取上端自幽门、下端至回盲部的肠管，置于托盘上，轻轻将小肠拉成直线进行测量。肠管长度为“小肠总长度”，从幽门至墨汁前沿为“墨汁推进长度”。按下列公式计算墨汁推进率(P)，并进行数据转换(X)后再统计分析。

$X={\sin }^{-1}\sqrt{P}$Each experimental group was orally given cold tea of the present invention by 1200, 2400, 3600mg/kg.BW (all adjusted to the same volume), and two matched groups were given distilled water of equal volume, once a day, continuously giving cold tea of the present invention for 7 days. Each group of animals fasted (drinking water is not limited) on the evening of the last sample giving, and after 16 hours, the model control group and 3 dosage groups were given a dose of compound diphenoxylate solution (0.2ml/10g.BW ), the negative control group was given distilled water. After 30 minutes, the ink containing herbal tea of the present invention (containing 5% activated carbon powder and 10% gum arabic) was given to the three dosage groups, and the ink was given to the model control group and the negative control group. After 25 minutes of ink administration, the animals were sacrificed by cervical dislocation, the abdominal cavity was opened to separate the mesentery, the upper end of the intestinal tube from the pylorus, the lower end to the ileocecal was cut, placed on a tray, and the small intestine was gently pulled into a straight line for measurement. The length of the intestinal tube is the "total length of the small intestine", and the length from the pylorus to the front of the ink is the "length of ink advancement". Calculate the ink propulsion rate (P) according to the following formula, and perform statistical analysis after data conversion (X).

$x={\sin }^{-1}\sqrt{P}$

1.5.2 排便时间、粪便粒数和粪便重量的测定1.5.2 Determination of defecation time, number of feces particles and weight of feces

各实验组按1200、2400、3600mg/kg.BW(均调至相同体积)经口给予本发明凉茶，两个对照组给予等体积的蒸馏水，每天1次，连续给药7天。末次给样的当天晚上各组动物禁食(饮水不限)，16小时后模型对照组和3个剂量组给予剂量为10mg/kg.BW剂量的复方地芬诺酯(0.2ml/10g.BW)，阴性对照组给予等量的蒸馏水。给复方地芬诺酯后30分钟，模型对照组和阴性对照组小鼠给予墨汁灌胃，3个剂量组给予含相应本发明凉茶的墨汁，同时开始计时。每只动物均单独饲养，并供给饲料和饮水，观察记录每只动物首次排黑便时间、5小时内排便粒数及重量。Each experimental group was orally given herbal tea of the present invention by 1200, 2400, 3600mg/kg.BW (all adjusted to the same volume), and two control groups were given equal volumes of distilled water, once a day, for 7 consecutive days. Each group of animals fasted (drinking water is not limited) on the evening of the last sample giving, and after 16 hours, the model control group and 3 dosage groups were given a dose of compound diphenoxylate (0.2ml/10g.BW) that was 10mg/kg. ), the negative control group was given the same amount of distilled water. 30 minutes after giving the compound diphenoxylate, the mice in the model control group and the negative control group were gavaged with ink, and the three dosage groups were given ink containing the corresponding herbal tea of the present invention, and the timing was started at the same time. Each animal was raised separately and supplied with feed and drinking water. The time of the first black stool discharge of each animal, the number and weight of defecated pellets within 5 hours were observed and recorded.

1.5.3 实验数据用SPSS13.0统计软件进行方差分析统计处理。1.5.3 The experimental data were processed by statistical analysis of variance with SPSS13.0 statistical software.

2 实验结果2 Experimental results

2.1 本发明凉茶对动物的一般情况和体重的影响2.1 The effect of herbal tea of the present invention on the general condition and body weight of animals

给本发明凉茶后，各组动物的饮食和活动正常，剂量组小鼠的粪便比对照组的软且未出现稀便。实验组小鼠在实验结束时体重与阴性对照组及模型对照组比较差异均无显著性意义(P>0.05)，表明本发明凉茶对小鼠体重增长无影响作用。见表1。After giving the herbal tea of the present invention, the diet and activity of each group of animals are normal, and the feces of the mice in the dose group are softer than those of the matched group and no loose stools appear. The body weight of the mice in the experimental group compared with the negative control group and the model control group at the end of the experiment had no significant difference (P>0.05), showing that the herbal tea of the present invention has no effect on the growth of the mouse body weight. See Table 1.

        表1 各组动物实验前后的体重比较(x±s)Table 1 Comparison of body weight before and after the experiment in each group of animals (x±s)

注：表中各组间比较差异均无显著性意义(P>0.05)Note: There is no significant difference between the groups in the table (P>0.05)

2.2 本发明凉茶对小鼠小肠运动的影响2.2 The effect of herbal tea of the present invention on mouse small intestine motility

便秘模型对照组小鼠的墨汁推进率显著低于阴性对照组(P<0.01)，表示便秘模型成立。高、中、低剂量组小鼠的墨汁推进率高于模型对照组，经数据转换后统计分析，本发明高、中、低剂量组与便秘模型对照组的差异有显著性意义(P<0.05)。见表2。The ink propulsion rate of the mice in the constipation model control group was significantly lower than that in the negative control group (P<0.01), indicating that the constipation model was established. The ink propulsion rate of high, middle and low dose group mice is higher than model control group, after data transformation statistical analysis, the difference of the present invention's high, middle and low dose group and constipation model control group has significant significance (P<0.05 ). See Table 2.

            表2 各组小鼠小肠推进运动的结果(x±s)         Table 2 The results of the small intestine propulsion movement of mice in each group (x±s)

注：P值为各组与便秘模型对照组比较。Note: P value is the comparison between each group and the constipation model control group.

2.3 本发明凉茶对小鼠排便时间、粪便粒数和粪便重量的影响2.3 Effects of herbal tea of the present invention on mouse defecation time, number of feces grains and weight of feces

便秘模型对照组小鼠首便时间大于阴性对照组(P<0.01)，而粪便的粒数及粪便重量均小于阴性对照组(P<0.01)，说明便秘模型成立。各剂量组的首便时间比便秘模型对照组的缩短，高、中、低剂量组与其相比差异均有显著性意义(P<0.01)；高、中、低剂量组粪便粒数均大于便秘模型对照组，且与便秘模型对照组的差异有显著性意义(P<0.05)；高、中、低剂量组的粪便重量大于便秘模型对照组，且与便秘模型对照组的差异有显著性意义(P<0.05)。见表3。The first defecation time of the mice in the constipation model control group was longer than that of the negative control group (P<0.01), while the number and weight of feces were less than those of the negative control group (P<0.01), indicating that the constipation model was established. The first stool time of each dose group was shorter than that of the constipation model control group, and there were significant differences between the high, medium and low dose groups (P<0.01); the number of feces in the high, medium and low dose groups was greater than that of the constipation model The model control group, and the difference with the constipation model control group was significant (P<0.05); the stool weight of the high, medium and low dose groups was greater than the constipation model control group, and the difference with the constipation model control group was significant (P<0.05). See Table 3.

         表3 各组小鼠排便时间、粪便粒数和粪便重量结果(x±s)     Table 3 Results of defecation time, number of feces particles and feces weight of mice in each group (x±s)

注：P值为各组与便秘模型对照组比较。Note: P value is the comparison between each group and the constipation model control group.

3 讨论3 Discussion

实验结果显示，本发明凉茶能够明显促进小鼠小肠运动，增加粪便粒数、粪便重量及缩短首次排便时间。表明本发明凉茶具有通便的作用。便秘是老年人最常见的胃肠道症状，尤其是老年人由于肠蠕动功能相应减弱，腹部肌肉松弛无力及摄入液体过少更容易发生便秘。本发明凉茶其药理作用不但作用于小肠，更主要在于大肠；而饮用凉茶能及时补充液体且又是我国较为普遍接受的养生食疗传统习惯，因此具有较好的实用价值。Experimental results show that the herbal tea of the present invention can obviously promote the movement of the small intestine of mice, increase the number of feces grains, the weight of feces and shorten the first defecation time. Show that cold tea of the present invention has laxative effect. Constipation is the most common gastrointestinal symptom in the elderly, especially the elderly are more prone to constipation due to the corresponding weakening of intestinal peristalsis, loose abdominal muscles and insufficient fluid intake. Its pharmacological effect of the herbal tea of the present invention not only acts on the small intestine, but more mainly lies in the large intestine; and drinking the herbal tea can replenish liquid in time and is a traditional habit of health-preserving food therapy generally accepted in my country, so it has better practical value.

具体实施方式Detailed ways

以下实施例所用ZTC1+1 II型天然澄清剂购自北京正天成澄清技术有限公司。The ZTC1+1 type II natural clarifier used in the following examples was purchased from Beijing Zhengtiancheng Clarification Technology Co., Ltd.

例1example 1

原料：生何首乌20份、蒲公英20份、枳实15份、决明子15份和白糖15份。Raw materials: 20 parts of raw Polygonum multiflorum, 20 parts of dandelion, 15 parts of Citrus aurantium, 15 parts of cassia seed and 15 parts of sugar.

制备：preparation:

(1)将所述原料按分别以下方法处理：(1) The raw materials are processed by the following methods respectively:

将白糖经过高温杀菌后，加入10倍水，通过蒸汽微热、充分搅拌溶解后，过滤后得白糖水；After the white sugar is sterilized at high temperature, add 10 times of water, heat it slightly with steam, stir and dissolve it thoroughly, and filter to get white sugar water;

将何首乌、蒲公英、枳实、决明子经过浸泡30分钟后，放入原料罐中，加入10倍水，煮沸2小时，抽出液体，再次在原料罐中加入10倍水，煮沸2小时，合并、过滤两次煎煮液；After soaking Polygonum multiflorum, Dandelion, Citrus aurantium and Cassia seeds for 30 minutes, put them into the raw material tank, add 10 times of water, boil for 2 hours, take out the liquid, add 10 times of water in the raw material tank again, boil for 2 hours, combine and filter double decoction;

(2)将经上述白糖水加入到所述的煎煮液中，进行充分搅拌；(2) adding the above-mentioned white sugar water into the described decoction liquid, and fully stirring;

(3)采用ZTC1+1 II型天然澄清剂澄清过滤：(3) Use ZTC1+1 Type II natural clarifier to clarify and filter:

配制ZTC1+1 II型天然澄清剂的A组分溶液：称取1Kg A组分细粉，加少量水，搅拌成糊状，再加水定容至100L，静置24h，搅拌，双层纱布过滤；Preparation of component A solution of ZTC1+1 type II natural clarifier: weigh 1Kg of component A fine powder, add a small amount of water, stir to form a paste, add water to make up to 100L, let stand for 24 hours, stir, and filter with double gauze ;

配制ZTC1+1 II型天然澄清剂的B组分溶液：称取1Kg B组分细粉，加少量1％冰醋酸，搅拌成糊状，再加1％冰醋酸定容至100L，静置24h，搅拌，双层纱布过滤；Prepare the B component solution of ZTC1+1 type II natural clarifier: weigh 1Kg B component fine powder, add a small amount of 1% glacial acetic acid, stir it into a paste, add 1% glacial acetic acid to make it to 100L, and let it stand for 24 hours , stirred, and filtered through double-layer gauze;

将6％药液体积的B组分溶液缓慢细流加入药液中，搅匀，再将8％药液体积的A组分缓慢细流加入药液中，搅匀，置60～70℃水浴中保温1h，然后常温静置24h，粗滤后离心，取上清液加纯净水至原药∶药液为1∶60；Add component B solution of 6% medicinal liquid volume into the medicinal liquid in a trickle slowly, stir well, then slowly add component A solution of 8% medicinal liquid volume into the medicinal liquid, stir well, put in a water bath at 60-70°C Incubate for 1 hour, then stand at room temperature for 24 hours, centrifuge after coarse filtration, take the supernatant and add pure water to make the ratio of original medicine: medicine solution 1:60;

(4)杀菌冷却：采用蒸汽进行杀菌，杀菌温度为120℃，保持30分钟，而后反压冷却至30～40℃，即可；(4) Sterilization and cooling: Sterilize with steam, the sterilization temperature is 120°C, keep for 30 minutes, and then cool to 30-40°C under back pressure.

(5)灌装封口：真空灌装，真空度控制在0.03～0.04Mpa，药液温度保持在60～65℃，罐装后及时封口。(5) Filling and sealing: vacuum filling, the vacuum degree is controlled at 0.03-0.04Mpa, the temperature of the liquid medicine is kept at 60-65°C, and it is sealed in time after filling.

例2Example 2

原料：生何首乌40份、蒲公英40份、枳实15份、决明子15份和白糖30份Raw materials: 40 parts of raw Polygonum multiflorum, 40 parts of dandelion, 15 parts of Citrus aurantium, 15 parts of cassia seed and 30 parts of sugar

制备：preparation:

(1)将所述原料按分别以下方法处理：(1) The raw materials are processed by the following methods respectively:

将白糖经过高温杀菌后，加入8倍水，通过蒸汽微热、充分搅拌溶解后，过滤后得白糖水；After the white sugar is sterilized at high temperature, add 8 times of water, heat it slightly with steam, fully stir and dissolve, and filter to get white sugar water;

将何首乌、蒲公英、枳实、决明子经过浸泡30分钟后，放入原料罐中，加入8倍水，煮沸2小时，抽出液体，再次在原料罐中加入8倍水，煮沸2小时，合并、过滤两次煎煮液；Soak Polygonum multiflorum, Dandelion, Citrus aurantium and Cassia seeds for 30 minutes, put them into the raw material tank, add 8 times of water, boil for 2 hours, take out the liquid, add 8 times of water in the raw material tank again, boil for 2 hours, combine and filter double decoction;

(2)将经上述白糖水加入到所述的煎煮液中，进行充分搅拌；(2) adding the above-mentioned white sugar water into the described decoction liquid, and fully stirring;

(3)采用ZTC1+1 II型天然澄清剂澄清过滤：(3) Use ZTC1+1 Type II natural clarifier to clarify and filter:

配制ZTC1+1II型天然澄清剂的A组分溶液：称取1Kg A组分细粉，加少量水，搅拌成糊状，再加水定容至100L，静置24h，搅拌，双层纱布过滤；Prepare the A-component solution of ZTC1+1II type natural clarifier: weigh 1Kg of A-component fine powder, add a small amount of water, stir to form a paste, add water to make up to 100L, let it stand for 24 hours, stir, and filter with double-layer gauze;

配制ZTC1+1 II型天然澄清剂的B组分溶液：称取1Kg B组分细粉，加少量1％冰醋酸，搅拌成糊状，再加1％冰醋酸定容至100L，静置24h，搅拌，双层纱布过滤；Prepare the B component solution of ZTC1+1 type II natural clarifier: weigh 1Kg B component fine powder, add a small amount of 1% glacial acetic acid, stir it into a paste, add 1% glacial acetic acid to make it to 100L, and let it stand for 24 hours , stirred, and filtered through double-layer gauze;

将6％药液体积的B组分溶液缓慢细流加入药液中，搅匀，再将8％药液体积的A组分缓慢细流加入药液中，搅匀，置60～70℃水浴中保温1h，然后常温静置24h，粗滤后离心，取上清液加纯净水至原药∶溶液为1∶30；Add component B solution of 6% medicinal liquid volume into the medicinal liquid in a trickle slowly, stir well, then slowly add component A solution of 8% medicinal liquid volume into the medicinal liquid, stir well, put in a water bath at 60-70°C Incubate for 1 hour, then stand at room temperature for 24 hours, centrifuge after coarse filtration, take the supernatant and add pure water to the original drug: solution ratio of 1:30;

(4)杀菌冷却：采用蒸汽进行杀菌，杀菌温度为120℃，保持30分钟，而后反压冷却至30～40℃，即可；(4) Sterilization and cooling: Sterilize with steam, the sterilization temperature is 120°C, keep for 30 minutes, and then cool to 30-40°C under back pressure.

(5)灌装封口：真空灌装，真空度控制在0.03～0.04Mpa，药液温度保持在60～65℃，罐装后及时封口。(5) Filling and sealing: vacuum filling, the vacuum degree is controlled at 0.03-0.04Mpa, the temperature of the liquid medicine is kept at 60-65°C, and it is sealed in time after filling.

例3Example 3

原料：生何首乌15份、蒲公英15份、枳实25份、决明子20份和白糖10份Raw materials: 15 parts of raw Polygonum multiflorum, 15 parts of dandelion, 25 parts of Citrus aurantium, 20 parts of cassia seed and 10 parts of sugar

制备：preparation:

(1)将所述原料按分别以下方法处理：(1) The raw materials are processed by the following methods respectively:

将白糖经过高温杀菌后，加入12倍水，通过蒸汽微热、充分搅拌溶解后，过滤后得白糖水；After the white sugar is sterilized at high temperature, add 12 times of water, heat slightly with steam, fully stir to dissolve, and filter to get white sugar water;

将何首乌、蒲公英、枳实、决明子经过浸泡30分钟后，放入原料罐中，加入12倍水，煮沸2小时，抽出液体，再次在原料罐中加入12倍水，煮沸2小时，合并、过滤两次煎煮液；After soaking Polygonum multiflorum, Dandelion, Citrus aurantium and Cassia seeds for 30 minutes, put them into the raw material tank, add 12 times of water, boil for 2 hours, take out the liquid, add 12 times of water in the raw material tank again, boil for 2 hours, combine and filter double decoction;

(2)将经上述白糖水加入到所述的煎煮液中，进行充分搅拌；(2) adding the above-mentioned white sugar water into the described decoction liquid, and fully stirring;

(3)采用ZTC1+1 II型天然澄清剂澄清过滤：(3) Use ZTC1+1 Type II natural clarifier to clarify and filter:

配制ZTC1+1 II型天然澄清剂的A组分溶液：称取1Kg A组分细粉，加少量水，搅拌成糊状，再加水定容至100L，静置24h，搅拌，双层纱布过滤；Preparation of component A solution of ZTC1+1 type II natural clarifier: weigh 1Kg of component A fine powder, add a small amount of water, stir to form a paste, add water to make up to 100L, let stand for 24 hours, stir, and filter with double gauze ;

配制ZTC1+1 II型天然澄清剂的B组分溶液：称取1Kg B组分细粉，加少量1％冰醋酸，搅拌成糊状，再加1％冰醋酸定容至100L，静置24h，搅拌，双层纱布过滤；Prepare the B component solution of ZTC1+1 type II natural clarifier: weigh 1Kg B component fine powder, add a small amount of 1% glacial acetic acid, stir it into a paste, add 1% glacial acetic acid to make it to 100L, and let it stand for 24 hours , stirred, and filtered through double-layer gauze;

将6％药液体积的B组分溶液缓慢细流加入药液中，搅匀，再将8％药液体积的A组分缓慢细流加入药液中，搅匀，置60～70℃水浴中保温1h，然后常温静置24h，粗滤后离心，取上清液加纯净水至原药∶药液为1∶40；Add component B solution of 6% medicinal liquid volume into the medicinal liquid in a trickle slowly, stir well, then slowly add component A solution of 8% medicinal liquid volume into the medicinal liquid, stir well, put in a water bath at 60-70°C Incubate for 1 hour, then stand at room temperature for 24 hours, centrifuge after coarse filtration, take the supernatant and add purified water to make the ratio of original medicine: medicine solution 1:40;

(4)杀菌冷却：采用蒸汽进行杀菌，杀菌温度为120℃，保持30分钟，而后反压冷却至30～40℃，即可；(4) Sterilization and cooling: Sterilize with steam, the sterilization temperature is 120°C, keep for 30 minutes, and then cool to 30-40°C under back pressure.

(5)灌装封口：真空灌装，真空度控制在0.03～0.04Mpa，药液温度保持在60～65℃，罐装后及时封口。(5) Filling and sealing: vacuum filling, the vacuum degree is controlled at 0.03-0.04Mpa, the temperature of the liquid medicine is kept at 60-65°C, and it is sealed in time after filling.

例4Example 4

原料：生何首乌20份、蒲公英20份、枳实5份、决明子5份和白糖10份Raw materials: 20 parts of raw Polygonum multiflorum, 20 parts of dandelion, 5 parts of Citrus aurantium, 5 parts of cassia seed and 10 parts of sugar

制备：preparation:

(1)将所述原料按分别以下方法处理：(1) The raw materials are processed by the following methods respectively:

将白糖经过高温杀菌后，加入10倍水，通过蒸汽微热、充分搅拌溶解后，过滤后得白糖水；After the white sugar is sterilized at high temperature, add 10 times of water, heat it slightly with steam, stir and dissolve it thoroughly, and filter to get white sugar water;

将何首乌、蒲公英、枳实、决明子经过浸泡30分钟后，放入原料罐中，加入10倍水，煮沸2小时，抽出液体，再次在原料罐中加入10倍水，煮沸2小时，合并、过滤两次煎煮液；After soaking Polygonum multiflorum, Dandelion, Citrus aurantium and Cassia seeds for 30 minutes, put them into the raw material tank, add 10 times of water, boil for 2 hours, take out the liquid, add 10 times of water in the raw material tank again, boil for 2 hours, combine and filter double decoction;

(2)将经上述白糖水加入到所述的煎煮液中，进行充分搅拌；(2) adding the above-mentioned white sugar water into the described decoction liquid, and fully stirring;

(3)采用ZTC1+1 II型天然澄清剂澄清过滤：(3) Use ZTC1+1 Type II natural clarifier to clarify and filter:

配制ZTC1+1 II型天然澄清剂的A组分溶液：称取1Kg A组分细粉，加少量水，搅拌成糊状，再加水定容至100L，静置24h，搅拌，双层纱布过滤；Preparation of component A solution of ZTC1+1 type II natural clarifier: weigh 1Kg of component A fine powder, add a small amount of water, stir to form a paste, add water to make up to 100L, let stand for 24 hours, stir, and filter with double gauze ;

配制ZTC1+1II型天然澄清剂的B组分溶液：称取1Kg B组分细粉，加少量1％冰醋酸，搅拌成糊状，再加1％冰醋酸定容至100L，静置24h，搅拌，双层纱布过滤；Prepare the component B solution of ZTC1+1II type natural clarifier: weigh 1Kg component B fine powder, add a small amount of 1% glacial acetic acid, stir it into a paste, add 1% glacial acetic acid to make it to 100L, let it stand for 24 hours, Stir and filter with double gauze;

将6％药液体积的B组分溶液缓慢细流加入药液中，搅匀，再将8％药液体积的A组分缓慢细流加入药液中，搅匀，置60～70℃水浴中保温1h，然后常温静置24h，粗滤后离心，取上清液加纯净水至原药∶溶液为1∶50；Slowly add 6% solution volume of component B solution into the solution in a trickle, stir well, then slowly add component A solution with 8% volume of the solution into the solution, stir well, put in a water bath at 60-70°C Incubate for 1 hour, then stand at room temperature for 24 hours, centrifuge after coarse filtration, take the supernatant and add pure water to the original drug: solution ratio of 1:50;

(4)杀菌冷却：采用蒸汽进行杀菌，杀菌温度为120℃，保持30分钟，而后反压冷却至30～40℃，即可；(4) Sterilization and cooling: Sterilize with steam, the sterilization temperature is 120°C, keep for 30 minutes, and then cool to 30-40°C under back pressure.

(5)灌装封口：真空灌装，真空度控制在0.03～0.04Mpa，料液温度保持在60～65℃，罐装后及时封口。(5) Filling and sealing: vacuum filling, the vacuum degree is controlled at 0.03-0.04Mpa, the temperature of the material and liquid is kept at 60-65°C, and it is sealed in time after canning.

例5Example 5

原料：生何首乌40份、蒲公英40份、枳实25份、决明子25份和白糖30份Raw materials: 40 parts of raw Polygonum multiflorum, 40 parts of dandelion, 25 parts of Citrus aurantium, 25 parts of cassia seed and 30 parts of sugar

制备：preparation:

(1)将所述原料按分别以下方法处理：(1) The raw materials are processed by the following methods respectively:

将白糖经过高温杀菌后，加入8～12倍水，通过蒸汽微热、充分搅拌溶解后，过滤后得白糖水；After the white sugar is sterilized at high temperature, add 8 to 12 times of water, heat it slightly with steam, stir and dissolve it thoroughly, and filter to get white sugar water;

将何首乌、蒲公英、枳实、决明子经过浸泡30分钟后，放入原料罐中，加入12倍水，煮沸2小时，抽出液体，再次在原料罐中加入12倍水，煮沸2小时，合并、过滤两次煎煮液；After soaking Polygonum multiflorum, Dandelion, Citrus aurantium and Cassia seeds for 30 minutes, put them into the raw material tank, add 12 times of water, boil for 2 hours, take out the liquid, add 12 times of water in the raw material tank again, boil for 2 hours, combine and filter double decoction;

(2)将经上述白糖水加入到所述的煎煮液中，进行充分搅拌；(2) adding the above-mentioned white sugar water into the described decoction liquid, and fully stirring;

(3)采用ZTC1+1 II型天然澄清剂澄清过滤：(3) Use ZTC1+1 Type II natural clarifier to clarify and filter:

配制ZTC1+1 II型天然澄清剂的A组分溶液：称取1Kg A组分细粉，加少量水，搅拌成糊状，再加水定容至100L，静置24h，搅拌，双层纱布过滤；Preparation of component A solution of ZTC1+1 type II natural clarifier: weigh 1Kg of component A fine powder, add a small amount of water, stir to form a paste, add water to make up to 100L, let stand for 24 hours, stir, and filter with double gauze ;

配制ZTC1+1 II型天然澄清剂的B组分溶液：称取1Kg B组分细粉，加少量1％冰醋酸，搅拌成糊状，再加1％冰醋酸定容至100L，静置24h，搅拌，双层纱布过滤；Prepare the B component solution of ZTC1+1 type II natural clarifier: weigh 1Kg B component fine powder, add a small amount of 1% glacial acetic acid, stir it into a paste, add 1% glacial acetic acid to make it to 100L, and let it stand for 24 hours , stirred, and filtered through double-layer gauze;

将6％药液体积的B组分溶液缓慢细流加入药液中，搅匀，再将8％药液体积的A组分缓慢细流加入药液中，搅匀，置60～70℃水浴中保温1h，然后常温静置24h，粗滤后离心，取上清液加纯净水至原药∶溶液为1∶30；Slowly add 6% solution volume of component B solution into the solution in a trickle, stir well, then slowly add component A solution with 8% volume of the solution into the solution, stir well, put in a water bath at 60-70°C Incubate for 1 hour, then stand at room temperature for 24 hours, centrifuge after coarse filtration, take the supernatant and add pure water to the original drug: solution ratio of 1:30;

(4)杀菌冷却：采用蒸汽进行杀菌，杀菌温度为120℃，保持30分钟，而后反压冷却至30～40℃，即可；(4) Sterilization and cooling: Sterilize with steam, the sterilization temperature is 120°C, keep for 30 minutes, and then cool to 30-40°C under back pressure.

(5)灌装封口：真空灌装，真空度控制在0.03～0.04Mpa，料液温度保持在60～65℃，罐装后及时封口。(5) Filling and sealing: vacuum filling, the vacuum degree is controlled at 0.03-0.04Mpa, the temperature of the material and liquid is kept at 60-65°C, and it is sealed in time after canning.

Claims (3)

1. A herbal tea for preventing and treating sub-health constipation is prepared from water and the following raw materials in parts by weight: 15-40 parts of raw fleece-flower root, 15-40 parts of dandelion, 5-25 parts of immature bitter orange, 5-20 parts of cassia seed and 10-30 parts of white sugar; the preparation method of the herbal tea comprises the following steps:

(1) the raw materials are treated according to the following method:

sterilizing white sugar at high temperature, adding 8-12 times of water, slightly heating by steam, fully stirring and dissolving, and filtering to obtain white sugar water;

soaking polygonum multiflorum, dandelion, immature bitter orange and cassia seed for 30 minutes, putting the soaked objects into a raw material tank, adding 8-12 times of water, boiling for 2 hours, extracting liquid, adding 8-12 times of water into the raw material tank again, boiling for 2 hours, combining the two decoction liquids, and filtering;

(2) preparing a liquid medicine: adding the white sugar water into the decoction, and fully stirring to obtain a liquid medicine;

(3) clarifying and filtering by adopting a ZTC1+1II type natural clarifying agent: preparing a component A solution and a component B solution in the clarifier according to a product specification of a ZTC1+1II type natural clarifier produced by Beijing Zhengtian Fining clarification technology Limited company, slowly and finely adding the component B solution with 6% of the volume of liquid medicine into the liquid medicine, uniformly stirring, slowly and finely adding the component A solution with 8% of the volume of liquid medicine into the liquid medicine, uniformly stirring, placing in a water bath at 60-70 ℃ for heat preservation for 1h, then standing at normal temperature for 24h, coarsely filtering, centrifuging, taking supernatant, and adding water until the mass of a raw medicine and the volume ratio of the liquid medicine are 1: 30-60;

(4) sterilization and cooling: sterilizing by adopting steam, wherein the sterilization temperature is 120 ℃, keeping for 30 minutes, and carrying out back pressure cooling to 30-40 ℃;

(5) filling and sealing: vacuum filling is carried out under the conditions that the vacuum degree is 0.03-0.04 Mpa and the temperature of the liquid medicine is 60-65 ℃, and sealing is carried out in time after canning.

2. The herbal tea for preventing and treating sub-health constipation according to claim 1, wherein the raw materials comprise: 15-30 parts of raw fleece-flower root, 15-30 parts of dandelion, 10-20 parts of immature bitter orange, 10-20 parts of cassia seed and 10-20 parts of white sugar.

3. The herbal tea for preventing and treating sub-health constipation according to claim 1, wherein the raw materials comprise: 20 parts of raw fleece-flower root, 20 parts of dandelion, 15 parts of immature bitter orange, 15 parts of cassia seed and 15 parts of white sugar.