CN100588367C - Ring platform for testing the learning and memory ability of mice and its control method - Google Patents

Abstract

The invention discloses an annular platform used for testing the learning and memory capacity of a mouse and a control method thereof. The annular platform comprises a support (1), a working platform(2), a camera (5), a sound box (6), an information acquisition controller (7), a computer (8), etc. The computer (8) outputs a control signal according to a designed test plan and displays test data and images on an operational interface. The technology and method having good versatility and expandability can not only be applied to the programmed control and test of the annular platform, but alsobe applied to other ethological apparatuses such as a water labyrinth, an autonomous activity apparatus, an exploration exercise tester, etc. after undergoing corresponding transformations.

Description

Translated fromChinese

用于对小鼠的学习记忆能力进行测试的环形平台及其控制方法Ring platform for testing the learning and memory ability of mice and its control method

技术领域technical field

本发明是一种对小鼠的学习记忆能力进行测试的行为学仪器，涉及一种能够对小鼠提供电刺激和红外检测功能的环形平台装置及其控制方法，用于医学生物学和药学领域的研究。The invention is a behavioral instrument for testing the learning and memory ability of mice, and relates to a ring platform device capable of providing electrical stimulation and infrared detection functions to mice and its control method, which is used in the fields of medical biology and pharmacy Research.

背景技术Background technique

小鼠有厌恶强光的特性。在明亮环境下，小鼠要寻找暗处躲藏。而要正确找到躲藏处，必须利用环境中的线索来进行空间定位。接受不同处理如药物或衰老作用的小鼠空间学习记忆的能力会发生变化。Mice have the characteristic of aversion to bright light. In a bright environment, mice seek dark places to hide. In order to find the hiding place correctly, the clues in the environment must be used for spatial positioning. The ability to learn and remember spatially changes in mice that receive different treatments, such as drugs or the effects of aging.

环形平台(circular platform，CP)由Barnes在1979年首次用于大鼠空间学习记忆能力的检测，故又称Barnes迷宫。它用于小鼠空间学习记忆能力的研究尚少。The circular platform (CP) was first used by Barnes in 1979 to test the spatial learning and memory ability of rats, so it is also called the Barnes maze. There are few studies on its use in spatial learning and memory in mice.

环形平台装置在平台直径和开孔数等参数上各不相同，如Bach小组的装置是直径122cm的白色圆台，周边有40个均匀分布的直径5cm的孔洞，其中的一孔下有一逃跑隧道可用于小鼠躲藏。但Pompl小组的装置是直径69cm的白色圆台围以15cm高的黑墙，平台的周边均匀分布的是16个直径为4.5cm的小孔。The circular platform devices vary in terms of platform diameter and the number of openings. For example, the Bach group’s device is a white circular platform with a diameter of 122 cm, and there are 40 holes with a diameter of 5 cm evenly distributed around it, and there is an escape tunnel under one of the holes. to hide from mice. But Pompl’s group’s installation is a white round platform with a diameter of 69 cm surrounded by a 15 cm high black wall, and 16 small holes with a diameter of 4.5 cm are evenly distributed around the platform.

传统的环形平台程序采用人工记录。记录的指标仅仅是错误数和潜伏期。错误数是指搜索无隧道孔的次数。潜伏期是小鼠找到正确平台孔道前所耗的时间。Traditional ring platform procedures are manually recorded. The only metrics recorded are error count and latency. The number of errors is the number of times no tunnel holes were searched. Latency is the time it takes for the mouse to find the correct platform channel.

人工记录的效率低、指标少、数据精度低。此时的试验手段单一，如不能提供电刺激的试验手段、不能测定联合性学习记忆能力、不能准确评价小鼠的活跃程度，也不能记录图像等。在进行老年鼠学习记忆能力的研究时，由于老年小鼠的恐惧和焦虑性水平增加，雌鼠若缺乏电刺激的手段，往往导致试验时间过长。因此，研制一种实用性强，检测精度高，能检测多种性质的学习记忆能力且提供更多的试验手段和数据的自动化环形平台有重要的实际意义和应用价值。Manual recording has low efficiency, few indicators, and low data accuracy. At this time, the test method is single, such as the test method that cannot provide electrical stimulation, cannot measure the ability of associative learning and memory, cannot accurately evaluate the activity level of the mouse, and cannot record images, etc. When studying the learning and memory ability of aged mice, due to the increased level of fear and anxiety in aged mice, if the female mice lack the means of electrical stimulation, the test time will often be too long. Therefore, it is of great practical significance and application value to develop an automatic circular platform that has strong practicability, high detection accuracy, can detect learning and memory abilities of various properties, and provides more test methods and data.

发明内容Contents of the invention

为解决现有的环形平台存在的问题，本发明提供一种用于对小鼠的学习记忆能力进行测试的环形平台及其控制方法，该环形平台解决了平台的自动检测记录问题，并可提供电刺激和红外检测功能。In order to solve the existing problems of the existing ring platform, the present invention provides a ring platform and its control method for testing the learning and memory ability of mice. The ring platform solves the problem of automatic detection and recording of the platform, and can provide Electrical stimulation and infrared detection function.

本发明的技术方案如下：Technical scheme of the present invention is as follows:

一种用于对小鼠的学习记忆能力进行测试的环形平台，其特征在于：该环形平台包括有安装于支架1上的工作平台2；为平台2提供声、光信号的音箱6和光源3、4；用于图像记录的摄像机5；完成平台控制和信息采集的信息采集控制器7；以及用于指挥与调度整个工作平台协调工作的计算机8和计算机8的操作界面；工作平台2周边均匀开有开孔9，在某几个开孔9的下方，安装有供小鼠躲藏的逃跑隧洞11，逃跑隧洞11是可以抽取下来的活动部件；开孔9和逃跑隧洞11内侧面均安装有红外线检测组件，工作平台2的表面镶嵌有用于对实验动物提供刺激的电极剌激栅12和用于检测实验开始时间点的动物活动探头13，在工作平台2上方的支架1上设光源3、4、摄像机5、音箱6；电极剌激栅12、动物活动探头13、红外检测组件、光源3、4均接入信息采集控制器7，信息采集控制器7、音箱6、摄像机5与计算机8相连接，计算机8按设计的实验方案输出控制信号，并将检测数据和图像显示于操作界面上。A ring-shaped platform for testing the learning and memory ability of mice is characterized in that: the ring-shaped platform includes a workingplatform 2 installed on a support 1; a sound box 6 and alight source 3 for providing sound and light signals for theplatform 2 , 4; thevideo camera 5 that is used for image recording; theinformation collection controller 7 that completes platform control and information collection; and thecomputer 8 and the operation interface ofcomputer 8 that are used to command and dispatch the whole work platform to coordinate work;work platform 2 periphery is uniform There areopenings 9, and undercertain openings 9,escape tunnels 11 for mice to hide are installed. Theescape tunnels 11 are movable parts that can be extracted; Infrared detection assembly, the surface of the workingplatform 2 is inlaid with anelectrode stimulation grid 12 for providing stimulation to experimental animals and ananimal activity probe 13 for detecting the start time point of the experiment, and alight source 3, 4.Camera 5, speaker 6;electrode stimulation grid 12,animal activity probe 13, infrared detection components,light source 3, 4 are all connected toinformation collection controller 7,information collection controller 7, speaker 6,camera 5 andcomputer 8 connected, thecomputer 8 outputs control signals according to the designed experimental scheme, and displays the detection data and images on the operation interface.

所述的用于对小鼠的学习记忆能力进行测试的环形平台，其特征在于所述的电极剌激栅12是在工作平台2表面等间距镶嵌的铜方线，所述的红外线检测组件由能生成多路不同频率的平行红外光的红外线接收与发射组件并排布置而成。The ring-shaped platform for testing the learning and memory ability of mice is characterized in that theelectrode stimulation grid 12 is a copper square wire inlaid at equal intervals on the surface of the workingplatform 2, and the infrared detection assembly is composed of Infrared receiving and emitting components that can generate multiple channels of parallel infrared light with different frequencies are arranged side by side.

对如权利要求1所述的用于对小鼠的学习记忆能力进行测试的环形平台的控制方法，其特征在于：在计算机8的统一指挥调度下，分成输出控制部分、输入控制部分二条线并行工作；在所述的输出控制部分，计算机8按照通过程序设置的实验方案驱动音箱6和信息采集控制器7工作；所述信息采集控制器7接收计算机8发出的灯光、电剌激控制信号的时序与强度数据后，通过内电路变换，送入光源3、4和电极剌激栅12；在输入控制部分，摄像机5所采集的图像信号通过采集卡进入计算机8，并显示在操作界面上；动物活动探头13、红外检测组件的输出信号首先进入信息采集控制器7，编码后传输至计算机8，最终显示在操作界面上，显示在操作界面上的数据和图像可以保存、回放、分析。在平台表面的中央设有用于探测动物进入的活动探头。To the control method of the circular platform that is used to test the learning and memory ability of mice as claimed in claim 1, it is characterized in that: under the unified command and dispatch ofcomputer 8, be divided into output control part, input control part two lines parallel work; in the described output control part, thecomputer 8 drives the sound box 6 and theinformation collection controller 7 according to the experimental scheme set by the program; After the time series and intensity data are converted by the internal circuit, they are sent to thelight sources 3, 4 and theelectrode stimulation grid 12; in the input control part, the image signal collected by thecamera 5 enters thecomputer 8 through the acquisition card, and is displayed on the operation interface; The output signals of theanimal activity probe 13 and the infrared detection component first enter theinformation acquisition controller 7, are encoded and transmitted to thecomputer 8, and finally displayed on the operation interface. The data and images displayed on the operation interface can be saved, played back, and analyzed. In the center of the platform surface there is a movable probe for detecting animal entry.

所述的活动探头用于检测动物是否进入工作平台，作为实验程序自动开始的标志。The active probe is used to detect whether the animal enters the working platform, as a sign that the experimental procedure starts automatically.

本发明的优点是：The advantages of the present invention are:

(1)、本发明的时序控制，故障检测，数据采集，图像识别等功能由计算机完成，具有很高的精度，并可建立数据库档案和统计分析，不需要繁琐的人工记录和统计，具有很高的工作效率。(1), functions such as sequence control of the present invention, fault detection, data collection, image recognition are finished by computer, have very high precision, and can set up database archives and statistical analysis, do not need loaded down with trivial details manual record and statistics, have very high precision High work efficiency.

(2)、本发明采用组件式结构，易于维护，具有良好的工程实用性。(2) The present invention adopts a modular structure, which is easy to maintain and has good engineering practicability.

(3)、本发明可以对实验的开始和结束自动识别，提高了实验的效率，并可以设计人工条件下难以达到的各种试验条件的复杂组合。(3), the present invention can automatically identify the start and end of the experiment, improves the efficiency of the experiment, and can design complex combinations of various test conditions that are difficult to achieve under artificial conditions.

(4)、在环形平台上实现了电剌激这一重要的实验手段，并且在保证剌激的有效性和人身安全性的前提下，可以实现复杂要求的电脉冲剌激。(4) The important experimental method of electric stimulation is realized on the ring platform, and under the premise of ensuring the effectiveness of stimulation and personal safety, electric pulse stimulation with complex requirements can be realized.

(5)、本发明所采用的技术和方法具有良好的通用性和可扩展性，它不仅可以用于环形平台的程序化控制和检测，经过相应的变形，在其它行为学仪器也可以使用，如水迷宫、自主活动仪、探索运动试验仪等。(5), the technology and method adopted in the present invention have good versatility and scalability, it can not only be used for the programmed control and detection of the ring platform, but also can be used in other behavioral instruments after corresponding deformation, Such as water maze, autonomous activity instrument, exploratory exercise test instrument, etc.

附图说明Description of drawings

图1是本发明用于小鼠的学习记忆能力测试的环形平台系统结构示意图。Fig. 1 is a schematic structural diagram of the ring platform system used in the learning and memory ability test of mice according to the present invention.

具体实施方式Detailed ways

图1中有：支架1、工作平台2、光源3、4、摄像机5、音箱6、信息采集控制器7、计算机8、开孔9、轨道10、逃跑隧洞11、电极刺激栅12、动物活动探头13。In Fig. 1 there are: bracket 1,working platform 2,light source 3, 4,camera 5, sound box 6,information collection controller 7,computer 8,opening 9,track 10,escape tunnel 11,electrode stimulation grid 12, animal activity Probe13.

参见图1。用于小鼠的学习记忆能力测试的环形平台，包括安装于支架1上的工作平台2；为平台2提供声、光信号的音箱6和光源3、4；用于图像记录的摄像机5；完成平台控制和信息采集的信息采集控制器7；以及用于指挥与调度整个工作平台协调工作的计算机8和它的操作界面。See Figure 1. The circular platform used for the learning and memory ability test of mice includes a workingplatform 2 installed on a support 1; a sound box 6 andlight sources 3 and 4 that provide sound and light signals for theplatform 2; avideo camera 5 for image recording; completed Aninformation collection controller 7 for platform control and information collection; and acomputer 8 and its operation interface for commanding and dispatching the coordinated work of the entire work platform.

所述工作平台2，周边均匀开有开孔9，在某几个开孔9的下方，安装有供小鼠躲藏的逃跑隧洞11，逃跑隧洞11是可以抽取下来的活动部件；开孔9和逃跑隧洞11的内侧面均安装有红外检测组件；所述工作平台2的表面镶嵌有用于对实验动物提供刺激的电极刺激栅12和用于检测实验开始时间点的动物活动探头13，在工作平台2上方的支架1上设有摄像机5、音箱6和光源3、4；所述电极刺激栅12、动物活动探头13、红外检测组件、光源3、4均接入信息采集控制器7；所述音箱6、摄像机5、信息采集控制器7与计算机8相连接，计算机8按设计的实验程序输出控制信号，并将检测数据和图像实时在操作界面上显示。The workingplatform 2 is evenly provided withopenings 9 on the periphery, and belowcertain openings 9,escape tunnels 11 for mice to hide are installed. Theescape tunnels 11 are movable parts that can be extracted; theopenings 9 and Infrared detection components are installed on the inner side of theescape tunnel 11; the surface of the workingplatform 2 is inlaid with anelectrode stimulation grid 12 for providing stimulation to the experimental animals and ananimal activity probe 13 for detecting the start time of the experiment. The bracket 1 above 2 is provided with avideo camera 5, a sound box 6 and alight source 3,4; theelectrode stimulation grid 12, theanimal activity probe 13, the infrared detection assembly, and thelight source 3,4 are all connected to theinformation acquisition controller 7; Speaker 6,camera 5,information acquisition controller 7 are connected withcomputer 8, andcomputer 8 outputs control signals according to the designed experiment program, and displays the detection data and images on the operation interface in real time.

所述的电极刺激栅12是在绝缘、耐水的平台材料表面，按槽间距3mm刨3mm×3mm的方槽，嵌入3mm×3mm尺寸的铜方线，表面磨平构成；栅间距可以调整，以试验小鼠在平台上能受到有效的电刺激为准；通过平台中的过孔，将电极刺激栅的引线引出于平台的下方。Theelectrode stimulation grid 12 is formed on the surface of an insulating and water-resistant platform material, planing a square groove of 3 mm × 3 mm according to a groove spacing of 3 mm, embedding a copper square wire of a size of 3 mm × 3 mm, and grinding the surface; the grid spacing can be adjusted to The test mice can receive effective electrical stimulation on the platform; through the via holes in the platform, lead the lead wire of the electrode stimulation grid to the bottom of the platform.

在输出部分，计算机8的总控模块读取方案库中的实验参数，通过通讯接口模块，驱动音箱和采集控制器7工作；采集控制器7接收到灯光、电刺激信号的时序、强度数据后，通过内电路变换，送入光源3、4和电极刺激栅12。在输入部分，摄像机5所采集的图像信号通过采集卡进入计算机8，并显示在操作界面上；动物活动探头13、平台各开孔9、逃跑隧洞11的红外检测信号首先进入采集控制器7，编码后传输至计算机8的总控模块，最终显示在操作界面上。显示在操作界面上的数据和图像可以保存，回放、分析。In the output part, the master control module of thecomputer 8 reads the experimental parameters in the program library, and drives the sound box and theacquisition controller 7 to work through the communication interface module; , through the internal circuit transformation, sent to thelight sources 3, 4 and theelectrode stimulation grid 12. In the input part, the image signal collected by thecamera 5 enters thecomputer 8 through the acquisition card, and is displayed on the operation interface; the infrared detection signal of theanimal activity probe 13, eachopening 9 of the platform, and theescape tunnel 11 first enters theacquisition controller 7, After encoding, it is transmitted to the master control module of thecomputer 8, and finally displayed on the operation interface. The data and images displayed on the operation interface can be saved, played back and analyzed.

Claims (3)

Translated fromChinese

1、一种用于对小鼠的学习记忆能力进行测试的环形平台，其特征在于：该环形平台包括有安装于支架(1)上的工作平台(2)；为工作平台(2)提供声、光信号的音箱(6)和光源(3)、(4)；用于图像记录的摄像机(5)；完成平台控制和信息采集的信息采集控制器(7)；以及用于指挥与调度整个工作平台协调工作的计算机(8)和计算机(8)的操作界面；工作平台(2)周边均匀开有开孔(9)，在某几个开孔(9)的下方，安装有供小鼠躲藏的逃跑隧洞(11)，逃跑隧洞(11)是可以抽取下来的活动部件；开孔(9)和逃跑隧洞(11)内侧面均安装有红外线检测组件，工作平台(2)的表面镶嵌有用于对实验动物提供刺激的电极刺激栅(12)和用于检测实验开始时间点的动物活动探头(13)，在工作平台(2)上方的支架(1)上设光源(3、4)、摄像机(5)、音箱(6)；电极刺激栅(12)、动物活动探头(13)、红外检测组件、光源(3、4)均接入信息采集控制器(7)，信息采集控制器(7)、音箱(6)、摄像机(5)与计算机(8)相连接，计算机(8)按设计的实验方案输出控制信号，并将检测数据和图像显示于操作界面上。1, a kind of annular platform that is used to test the learning and memory ability of mouse, it is characterized in that: this annular platform includes the working platform (2) that is installed on the support (1); Provide sound for working platform (2) , speaker (6) and light source (3), (4) for optical signal; camera (5) for image recording; information collection controller (7) for platform control and information collection; and for commanding and dispatching the entire The computer (8) and the operation interface of the computer (8) that the work platform coordinates work; The periphery of the work platform (2) is evenly provided with openings (9), and below some openings (9), a mouse The hidden escape tunnel (11), the escape tunnel (11) is a movable part that can be extracted; the opening (9) and the inner side of the escape tunnel (11) are equipped with infrared detection components, and the surface of the working platform (2) is embedded with useful For the electrode stimulation grid (12) that provides stimulation to the experimental animals and the animal activity probe (13) used to detect the start time point of the experiment, light sources (3, 4), light sources (3, 4), Camera (5), sound box (6); electrode stimulation grid (12), animal activity probe (13), infrared detection component, light source (3, 4) are all connected to information collection controller (7), and information collection controller ( 7), the sound box (6), the video camera (5) are connected with the computer (8), and the computer (8) outputs control signals according to the designed experimental scheme, and displays the detection data and images on the operation interface.2、根据权利要求1所述的用于对小鼠的学习记忆能力进行测试的环形平台，其特征在于所述的电极刺激栅(12)是在工作平台(2)表面等间距镶嵌的铜方线，所述的红外检测组件由能生成多路不同频率的平行红外光的红外线接收与发射组件并排布置而成。2. The ring-shaped platform for testing the learning and memory ability of mice according to claim 1, characterized in that the electrode stimulation grid (12) is a copper square inlaid at equal intervals on the surface of the working platform (2). The infrared detection component is composed of infrared receiving and emitting components that can generate multiple channels of parallel infrared light with different frequencies arranged side by side.3、一种对如权利要求1所述的用于对小鼠的学习记忆能力进行测试的环形平台的控制方法，其特征在于：在计算机(8)的统一指挥调度下，分成输出控制部分、输入控制部分二条线并行工作；在输出控制部分，计算机(8)按照通过程序设置的实验方案驱动音箱(6)和信息采集控制器(7)工作；信息采集控制器(7)接收计算机(8)发出的灯光、电刺激控制信号的时序与强度数据后，通过内电路变换，送入光源(3、4)和电极刺激栅(12)；在输入控制部分，摄像机(5)所采集的图像信号通过采集卡进入计算机(8)，并显示在操作界面上；动物活动探头(13)、红外检测组件的输出信号首先进入信息采集控制器(7)，编码后传输至计算机(8)，最终显示在操作界面上，显示在操作界面上的数据和图像可以保存、回放、分析。3, a kind of control method to the circular platform that is used for testing the learning and memory ability of mouse as claimed in claim 1, it is characterized in that: under the unified command and dispatch of computer (8), be divided into output control part, The two lines of the input control part work in parallel; in the output control part, the computer (8) drives the sound box (6) and the information collection controller (7) to work according to the experimental scheme set by the program; the information collection controller (7) receives the computer (8) ) after the timing and intensity data of the lights and electric stimulation control signals sent by the internal circuit are converted and sent to the light source (3, 4) and the electrode stimulation grid (12); in the input control part, the image collected by the camera (5) The signal enters the computer (8) through the acquisition card, and is displayed on the operation interface; the output signal of the animal activity probe (13) and the infrared detection component first enters the information acquisition controller (7), is encoded and then transmitted to the computer (8), and finally Displayed on the operation interface, the data and images displayed on the operation interface can be saved, played back and analyzed.

Images