w0 99/25336 PCT/US98/24606 METHODS FOR PREVENTING
RESTENOSIS jISING TOCOTRIENOLS
TECHNICAL FIELD OF THE INVENTION
This invention relates to the prevention of restenosis using tocotrienols.
Specifically, this invention relates to the use of individual tocotrienols (such as P25 tocotrienol), mixtures of tocotrienols and mixtures of one or more tocotrienols with other substances (such as to the TRF25 mixture). The methods of this invention are particularly well suited for preventing restenosis in patients having undergone or prior to undergoing angioplasty or arterial bypass surgery.
An estimated 905,000 patients are at high risk for restenosis. An artery that is constricted or narrowed is referred to as being stenosed. The artery may be clogged by the buildup over time of fat, cholesterol and other substances that adhere to the interior of arterial 2o walls. Procedures designed to unblock such clogged arteries result in localized arterial trauma. The result of such damage to the artery is an increased risk for atheroma formation at the site of trauma (restenosis).
One procedure for widening blocked coronary arteries is percutaneous transluminal 25 coronary angioplasty (PTCA), also known as balloon angioplasty. PTCA
involves the use of a balloon-tipped catheter that is inserted directly into the heart's vessels to open partially blocked, or stenotic, coronary arteries. Over 400,000 PTCA
procedures were performed in 1994 alone in the United States. The average cost of the PTCA
procedure well exceeds $20,000. Unfortunately, PTCA often does not yield long term success in 3o unblocking clogged arteries. About one-third of patients undergoing PTCA
develop restenosis of the widened segment within about six months of the procedure.
Patients suffering from restenosis may have to undergo additional procedures to reduce the blockage, including repeated angioplasties, atherectomies or the insertion of stents (collapsible supports placed inside the arteries). Collectively, these additional procedures add billions of dollars to the cost of health care. For example, in 1994, repeat angioplasty alone was responsible for about $2.4 billion of additional health costs in the United States.
Restenosis can also occur following other procedures that cause damage or trauma to an artery, such as arterial bypass operations (including coronary artery bypass procedures).
Arterial bypass surgery reroutes the flow of blood around clogged arteries via an arterial 1o transplant or graft to improve the supply of blood and oxygen to the heart and other vital organs. In such cases, stenosis sometimes occurs in the transplanted blood vessel' segment. Like other stenosed arteries, angioplasty or atherectomy may be required to widen the passage. Because of the arterial trauma associated with arterial bypass surgery, restenosis is a substantial risk after such procedures.
A variety of approaches to restenosis prevention are being explored. These include administration of antibodies against platelet antigen, use of an epidermal growth factor fusion protein, gene therapy, nitrogen oxide based therapy, acyclovir therapy and use of sulfated cyclodextrins. In addition, use of catheter-based intracoronary radiotherapy has 2o been shown to reduce the intimal hyperplasia that is a part of restenosis in a preliminary, short term study (P.S. Teirstein et al., The New,~_nglarnd Journal of Medicine, 336(24), pp. 1697-703). The efficacy of antioxidants in preventing restenosis has also been preliminarily explored. There is some evidence to suggest that oxidizing metabolites generated at the site of coronary angioplasty induce a chain reaction that can lead to restenosis. Orally administered alpha-tocopherol (vitamin E) has been shown to reduce restenosis after angioplasty in rabbits (A.M. Lafont et aL, J. Clin. Invest., 95(3), pp. 1018-25 (1995)). However, a more recent study debates the efficacy of antioxidants in preventing restenosis (J.C. Tardif et aL, New-gland Journal of Medicine, 337(6), pp.
365-72 (1997)). The Tardif et al. article notes that although one antioxidant (probucol (Lorelco, Merrell, Kansas City, Mo.)) was somewhat effective in preventing restenosis, other antioxidants did not prevent restenosis. Furthermore, the combination of probucol with other antioxidants was shown to be less effective than probucol alone in preventing restenosis. Finally, Tardif et al. propose that the antioxidant properties of probucol may not be related to its prophylactic activity with respect to restenosis (as is the case with its atherosclerosis and vascular reactivity profile).
w0 99/25336 PCT/US98/24606 Notwithstanding extensive research conducted in this area, there is still a well-recognized and unmet need for effective therapy to prevent restenosis.
SUMMARY OF THE INVENTION
The present invention satisfies the need for therapeutic agents effective in the prevention of restenosis. Specifically this invention provides methods for preventing restenosis in a patient comprising the step of administering to the patient a prophylactically effective amount of a composition comprising a tocotrienol, a mixture of tocotrienols or a mixture of one or more tocotrienols with other substances.
This invention also provides a method for preventing restenosis in a patient undergoing an arterial angioplastic procedure comprising the steps of:
{a) coating the exterior surface of an angioplastic balloon with a composition comprising a tocotrienol; and (b) performing the arterial angioplastic procedure such that a prophylactically effective amount of the composition is transferred to the interior surface of the artery.
The tocotrienols useful in the methods of this invention include those compounds possessing the following three structural characteristics: (1) a hydrogen donor group (or a group that can be hydrolyzed to a hydrogen donor group) attached to an aromatic ring system; (2) a side chain attached to the aromatic ring system comprising one or more isoprenoid or isoprenoid-like units and (3) a methylene unit or a functional group having at least one lone pair of electrons positioned adjacent to the atom to which the side chain is attached to the aromatic ring, said electrons being conjugated to the aromatic ring system. Specific preferred tocotrienols useful in the methods of this invention include 3o desmethyl-tocotrienol, a- tocotrienol, ~3- tocotrienol, Y- tocotrienol, 8-tocotrienol, P, 8 tocotrienol and P25 tocotrienol.
VlCO 99/25336 PCT/US98/Z4606 BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 shows the effects ofy- tocotrienol (GT301) on superoxide production in human peripheral blood neutrophils.
DETAILED DESCRIPTION OF THE INVENTION
As used herein, the following definitions apply {unless expressly noted to the contrary):
"Composition" as used herein refers to a preparation for administration via any acceptable route known to those of ordinary skill in the art. Such routes include, but are not limited to oral, parenteral, intravenous or topical administration.
"Composition"
encompasses pharmaceutical compositions as well as dietary supplements, foodstuffs, food additives and the like.
"Desmethyl-tocotrienol" refers to the compound 3,4-dihydro-2-methyl-2-(4,8,12-trimethyltrideca-3'(E), 7'(E), 11'-trienyl)-2H-benzopyran-6-ol. This specific tocotrienol has been referred to as "tocotrienol" in some of the published literature cited herein.
"Patient" refers to a warm-blooded mammal and preferably, a human. Patients in need of prophylactic therapy to prevent restenosis are those patients possessing one or more risk factors for the development of restenosis. For example, patients who have undergone angioplasty or arterial bypass surgery are at particular risk for the development of restenosis. Advantageously, patients may also receive prophylactic restenosis therapy according to this invention (short or long-term) prior to undergoing angioplasty or arterial bypass surgery.
"P~8 tocotrienol" refers to a tocotrienol having the formula HO
HOC
P18 tocotrienol and P~$ are trademarks of Bionutrics, Inc. (Phoenix, Arizona).
"P2s tocotrienol" refers to the tocotrienol 3,4-dihydro-2-(4,8,12-trimethyltrideca-3'(E),7'(E), 11"-trienyl)-2H-1-benzopyran-6-ol) which has the formula HO
This compound is also known as didesmethyl-tocotrienol. Pzs tocotrienol and P2s are trademarks of Bionutrics, Inc. (Phoenix, Arizona).
"Prophylactically effective amount" refers to an amount of active ingredient sufficient to to prevent restenosis or the symptoms of restenosis in a patient for a period of at least about six months. "Prophylactically acceptable means" refers to means effective to impart a prophylactic effect.
"Tocotrienol" refers to compounds possessing the following three structural 15 characteristics: (1) a hydrogen donor group (or a group that can be hydrolyzed to a hydrogen donor group) attached to an aromatic ring system; (2) a side chain attached to the aromatic ring system comprising one or more isoprenoid or isoprenoid-like units and (3) a methylene unit or a functional group having at least one lone pair of electrons positioned adjacent to the atom to which the side chain is attached to the aromatic ring, 2o said electrons being conjugated to the aromatic ring system (preferably CH2, C=O, CHOH, O, S or NH). Preferred tocotrienols for use in the methods of this invention are those which are naturally occurring. These naturally occurring tocotrienols may be conveniently isolated from biological materials or synthesized from commercially available starting material. Preferably, the tocotrienols for use in the methods of this 25 invention are obtained from biological materials that have been stabilized and extracted, such as by the processes described in PCT publication WO 91/17985 (the entire disclosure of which is hereby incorporated by reference). Examples of preferred biological materials, tocotrienols and methods for obtaining tocotrienols synthetically and from biological materials are referred to in co-owned US patent 5,591,772 and PCT
3o publication WO 91/17985 (the entire disclosures of which are hereby incorporated by reference). Preferred biological materials from which the tocotrienols of this invention may be obtained include stabilized brans and especially, stabilized rice bran.
w0 99/25336 PCT/US98/24606 Specific preferred tocotrienols of this invention include those of formula (I):
R~
R~
~ccH zM
w 3 Rs R~
Re wherein R, and R3 are each independently selected from the group consisting of H, halogen, OH, OCH~ and Ct-C6 branched or unbranched alkyl (preferably, H, halogen and Cl-C3 branched or unbranched alkyl and more preferably, H and methyl);
1o R2 is a hydrogen donor group selected from the group consisting of OH, NHRB, COZY, C(R8)ZCOZH and C1-C8 branched or unbranched alkyl substituted with OH, NHRg, COZY
or C(Rg)2COZH (preferably, OH and C1-C3 branched or unbranched alkyl substituted with OH and more preferably, OH);
R4 is selected from the group consisting of O, NH, CH-Rg, C=O and CH-OH
(preferably, i 5 O, CHZ and C=O);
RS is selected from the group consisting of CHZ, C=O, CHOH, O, S and NH
(preferably, O, CHZ and C=O and more preferably, O and C=O);
R6 is selected from the group consisting of H and C,-C6 branched or unbranched alkyl (preferably, H and C1-C3 branched or unbranched alkyl and more preferably, H
and 2o methyl);
R~ is selected from the group consisting of isoprenoid and isoprenoid-like side chains, and more preferably from the group consisting of side chains of formulas (a)-(c):
Rio ", R" (a) Rio p m R» (b) and VltO 99/25336 PCT/US98/24686 R ~o O!-~' R ~ t o (c) wherein each R,o is independently selected from the group consisting of H, NH2 and C~-C6 branched or unbranched alkyl and Rl ~ is selected from the group consisting of H, Ct-C6 branched or unbranched alkyl, CH20H, C02H and OH (preferably, R~ is a side chain of formula (a), wherein Rlo and R> > are each independently is selected from the group consisting of H and C~-C3 branched or unbranched alkyl and more preferably, H
and methyl);
1o each R8 and R9 is independently selected from the group consisting of H and C!-C6 branched or unbranched alkyl (preferably, H and C~-C~ branched or unbranched alkyl and more preferably, H and methyl);
Y is H or and C,-C,g branched or unbranched alkyl (preferably H and C~-C6 branched or unbranched alkyl and more preferably, H and C1-C4 branched or unbranched alkyl);
Z is selected from the group consisting of H, halogen, OH, CH20H, CH3, OCH3 and COCH3 (preferably H and CH3);
n is an integer selected from the group consisting of 0,1, 2, 3 and 4 (preferably 0 and 1);
and m is an integer selected from the group consisting of 1-30 (preferably 1-20, more 2o preferably 3-10 and most preferably, 3-7).
More preferred tocotrienols of this invention include desmethyl-tocotrienol, a-tocotrienol, ~3- tocotrienol, y- tocotrienol, b- tocotrienol, P18 tocotrienol and P2s tocotrienol.
This invention expressly encompasses the prodrug form of tocotrienols. Upon administration to a patient, such a prodrug undergoes biotransformation to their active form. Prodrugs include the esterified form of the tocotrienols used in this invention which comprise a carboxylic acid functionality.
The tocotrienols for use in the methods of this invention may be in their isomerically pure form or be present as mixtures of isomers. For example, the tocotrienols of this invention VftO 99/25336 PCT/US98/24606 may exist as the d- or 1-isomer or the d,l-racemic mixture. The naturally occurnng isomer (usually the d-isomer) and the d,l-racemic mixture are preferred.
"TRF" refers to a tocotrienol rich fraction obtained by the stabilization and extraction of a biological source. TRF typically contains varying amounts of desmethyl-tocotrienol,. a-tocotrienol, Vii- tocotrienol, r- tocotrienol and 8- tocotrienol and may also contain quantities of the newly discovered tocotrienols, P~8 tocotrienol and Pzs tocotrienol. Most commonly, TRF will be comprise at least about SO% to about 90% tocotrienols w/w (preferably, at least about 60% to about 90% and more preferably, at least about 70% to to about 90%).
"TRFzs" refers to a TRF comprising a significant weight percentage of Pzs tocotrienol.
Preferably, TRFzs comprises at least about S% Pzs, more preferably, at least about 10%
Pzs, and even more preferably, at least about 1 S% Pzs w/w. An example of the 15 preparation of a specific TRFzs is set forth in A.A. Qureshi et al., Nutr.
Biochern., 8, pp.
290-98 (1997). TRFzs is a preferred component of the compositions and methods described herein. TRFzs is a trademark of Bionutrics, Inc. (Phoenix, Arizona).
One embodiment of this invention provides a method for preventing restenosis in a 2o patient comprising the step of administering to the patient a prophylactically effective amount of a composition comprising a tocotrienol, a mixture of tocotrienols or a combination of one or more tocotrienols with one or more additional substances. This embodiment of the invention provides an effective means for administering tocotrienols systemically or locally to a patient in need of restenosis prophylaxis.
An alternate embodiment of this invention provides a method for preventing restenosis in a patient comprising the steps of (a) coating the exterior surface of an angioplastic balloon with a composition 30 comprising a tocotrienol; and (b) performing the arterial angioplastic procedure such that a prophylactically effective amount of the composition is transferred to the interior surface of the artery.
Such a coating also may be applied to implantable and non-implantable vascular instruments (e.g., stents).
This alternate embodiment of the invention provides an effective means for delivering tocotrienols directly to the site of damage where restenosis is likely to develop without necessitating further invasive procedures. Delivery of the tocotrienols in this way reduces the severity of endothelial damage caused as a result of the trauma and also reduces further damage resulting from the subsequent inflammatory response.
The methods described herein may be used alone or in conjunction with conventional prophylactic methods (such as those described above). Accordingly, the methods of this invention, used alone or together with other methods, provide a safe and effective means for reducing or eliminating the risk of restenosis in a patient in need of such prophylactic treatment.
Without wishing to be bound by theory, tocotrienols are useful in preventing restenosis due to their unique combination of antioxidant, antiinflammatory and antithrombotic properties. As opposed to conventional therapeutics that target a single mechanism, tocotrienols target multiple mechanisms leading to restenosis. For example, the 2o tocotrienols of this invention inhibit the production of free arachidonic acid (a major mediator of inflammatory response). This inhibition is believed to occur by either the inhibition of phospholipase A2 or alternatively, through the increase in corticosterone levels in the blood. Phospholipase A2 cleaves at C-2 of phosphate head groups, resulting in the release of free arachidonic acid. Free arachidonic acid can then be converted to a variety oi'biologically important molecules, such as prostaglandins and thromboxanes (via the cyclooxygenase pathway) and the leukotrienes (via the lipoxygenase pathway).
These factors are associated with the increased platelet aggregation and vasoconstriction in restenosis.
3o Furthermore, tocotrienols inhibit the production of a variety of cytokines (including TNF, IL-1 and growth factors). These cytokines contribute to the proliferation of smooth muscle and propagation of the inflammatory response associated with restenosis.
Furthermore, tocotrienols reduce the levels of superoxide production.
Superoxide and nitric oxide react to form peroxynitrite, which is a causative factor in endothelial damage and arteriosclerosis. By reducing superoxide and cytokine production, tocotrienols reduce the cell proliferation, chemotaxis, inflammation and endothelial damage that are w0 99/25336 PCT/US98/24606 responsible for restenosis. As a result of their unique combination of properties, tocotrienols are capable of combating the root causes and lessen the severity of the arterial response to injury that often leads to restenosis.
Compositions of this invention are prepared by combining one or more tocotrienols with an acceptable carrier. For pharmaceutical compositions of this invention, the carrier must be pharmaceutically acceptable (i.e., a carrier which is non-toxic to the patient at the administered level and which does not destroy the activity of the active components) of the composition). Acceptable carriers, including pharmaceutically acceptable carriers, are 10 well known to those of ordinary skill in the art.
The compositions of this invention may be used or administered by any prophylactically acceptable means to a patient in need of restenosis prophylaxis. For example, pharmaceutical compositions of this invention may be administered orally, topically, transdemlally, parenterally, intravenously or by inhalation. These compositions may be formulated so as to impart a time-released benefit. Oral compositions may take the form of tablets, capsules, caplets, emulsions, liposomes, suspensions, powders and the like.
Topical compositions include, but are not limited to, gels, lotions and creams. Parenteral compositions take the form of sterile solutions and emulsions and the like.
Intravenous 2o compositions include, but are not limited to sterile solutions. The preferred routes of administration are parenteral injection of a sterile solution or emulsion (if being administered to a patient immediately prior to undergoing angioplasty or arterial bypass surgery) and oral or transdermal administration (if being administered to patients who have already undergone angioplasty or arterial bypass surgery or who will be treated for a prolonged period of time prior to any such procedure).
Suitable carriers for use in the alternate embodiment of this invention are pharmaceutically acceptable carriers that are sufficiently viscous to adhere to the exterior surface of an angioplastic balloon but that are readily transferred to the interior surface of the treated artery upon inflation during the angioplastic procedure. The exterior surface of the angioplastic balloon can be pretreated or altered (such as by mechanical or chemical roughening or increasing the surface porosity) to enhance the ability of the tocotrienol composition to adhere to the surface. Suitable carriers include glycols, such as propylene glycol, parabens (such as methyl and propyl), glycerin, alcohols, petrolatum, oils (including rice bran and other bran oils) and waxes. Propylene glycol and rice bran oil are preferred carriers for this method. Other suitable carriers (including additives and adjuvants) are well known to those of ordinary skill in the art. Typically, a formulation comprising about 0.1 - 5000 mg of a tocotrienol composition (comprising from about 0.1 % to about 50%, preferably from about 1 % to about 25% and more preferably, about 5% to about 15% w/w tocotrienol) and about 0.1 to about 100 g of a suitable carrier (preferably about 1 to about 50 g and more preferably, from about 1 to about 25 g of a suitable earner) is used to coat the balloon prior to the angioplastic procedure. The balloon may be coated immediately preceeding surgery or may be pre-coated and stored for later use.
to Dosage levels and requirements are well-recognized in the art and may be chosen by those of ordinary skill in the art from publicly available sources. Typically, dosage levels will range between about 0.1 and about 10,000 mg of tocotrienol or mixture of tocotrienols per dose. Preferably, the range is between about 0.1 and about 5,000 mg of active ingredient per dose. If being administered to patients who have undergone 15 angioplasty or arterial bypass surgery, multiple doses may be required over a period of time to obtain maximum benefit. For example, a patient may receive oral administration of between about 0.1 and about 5000 mg/day for a period of several weeks to several months following angioplasty or bypass surgery. Patients receiving prophylactic treatment prior to undergoing angioplasty or arterial bypass surgery may be administered, 20 ~ for example, one or more doses of between about 0.1 and about 5000 mg/day by parenteral injection for one or more days or weeks prior to the procedure. For the alternate embodiment of this invention, a concentration should be selected such that a prophylactically effective amount of the tocotrienol composition is transferred to the interior surfaces of the treated artery. Specific dosage and treatment regimens will 25 depend upon factors such as the patient's overall health status, the severity and course of the patient's disorder or disposition thereto and the judgment of the treating physician.
Higher or lower doses may be employed as needed.
Tocotrienols and mixtures thereof may be used in combination with conventional 3o therapeutics in the methods described herein. The conventional therapeutics may be administered separately from the tocotrienols and mixtures thereof, or they may be formulated together in a single dosage form. Such combination therapy advantageously utilize lower dosages of those conventional therapies and reduce or avoid possible toxicity incurred when those agents are used as monotherapies. For example, the 35 tocotrienols used in the methods of this invention may be used in conjunction with any anti-inflammatory or anti-oxidative agent. Preferably, in combination therapy, the w0 99/25336 PCT/US98/24606 tocotrienols are used in conjunction with non-steroidal anti-inflammatory agents (NSAIDS) or with one or more of the following restenosis prophylactic agents:
ReoPro (Centacor, Inc.), DAB389EGF (Seragen, Inc.), gene-based anti-restenosis agents (such as those being developed by Antivirals, Inc.), nitrogen oxide based anti-restenosis agents (such as those being developed by NitroMed, Inc.), acyclovir, acyclovir with the thymidine kinase gene (CardioGene Therapeutics, Inc.) or cyclodextrins (such as the sulfated cyclodextrins being developed by Atlantic Pharmaceuticals, Inc.).
Particularly preferred combination therapies employ the co-administration of the tocotrienol compositions of this invention with aspirin, naproxen salts and ibuprofen (and more preferably, aspirin).
EXAMPLES
In order that this invention be more fully understood, the following examples are set forth. These examples are for the purpose of illustration only and are not to be construed as limiting the scope of the invention in any way.
The methods used for obtaining and purifying tocotrienols and mixtures thereof (including TRF) useful for the methods of this invention are described in the Examples section of US patent 5,591,772. Stabilization of rice bran follows Example 1 of US
patent 5,591,772. Purification of TRF, P25 tocotrienol and P18 tocotrienol follow Examples 2-4 of US patent 5,591,772.
All assays conducted on chicken or swine were done following the protocols described in A.A. Qureshi et al., Am J~~lin. Nutr., 53, pp. 1021S-26S (1991). All enzymatic assays were conducted following the protocols described in A.A. Qureshi et al., , 17, p.
924 (1982). TRF levels were measured using the radioimmunoassay kit available from Genzyme Corp. (Cambridge, MA).
The following specific protocols are used in the subsequent examples herein:
Protocol I: Dr;r Heat Stabilization Extruder: Wenger Model X-25 Standard ScrewBarrel Setup T
Barrel No. Standard Port Screw No. Standard Port 28714-9. 5 28320-1 Die/Spacer Measurement Standard Port Spacer 0.375 28340-11 Back Plate 0.625 28361-51 Intermediate Plate 0.218 28316-723 Front Plate 0.235 28389-507 erating Conditions Feed Rate: 1000 lbs/hr Temperature: 170C at exit die Pressure: 975-1025 psi Moisture Feed: 12%
Moisture Discharge: 9.6%
Residence Time: 15 seconds Run Duration: 8 hours Sample Size: 50 lbs VI~O 99/25336 PCT/US98/24606 Protocol II: Drv Heat Followed Bv Wet Heat Stabilii~t~~~
Dry Heat Stage: Protocol I
Wet Heat Stage:
Extruder: Anderson 4 inch Screw Barrel Configuration: Standard Cut Flight Die Setup:
Diameter: 0.1875 inches Length: 0.75 inches to Operating Conditions:
Feed Rate: 378 lbs/hr Shaft Speed: 279 ipm Steam Injection: 35 lbs/hr (32 psi at #8 hole) Mechanical Pressure: 750 psi (ast.) Moisture Feed: I 1.4%
Discharge Moisture: 15%
Discharge Rate: 450 lbs/br Discharge Temp.: 121 C
Protocol III: Dnr'Lag/ ooling Procedure The wet heat stabilized product of protocol II (15% moisture) was discharged onto aluminum trays and placed in a tray oven at 101.1°C until the moisture content was 8-10% (approximately 1.5 hrs). The trays were then placed on tray racks and allowed to cool at ambient temperature (approximately 20°C).
Protocol IV: Oil Extraction Oil to Hexane Ratio: 1:4 No. of Washings: 3 Extraction Temperature 40°C
The hexane was removed from the extract by mild heating (40°C) under a mild vacuum.
VI~O 99/25336 PCT/US98/24606 i5 Protocol V: Dewaxin~
20 lbs of crude oil were refrigerated for 24 hrs at -15.6°C. The supernatant (containing the dewaxed oil) was decanted from the solidified waxes. The waxes were then centrifuged to removed entrained oil, yielding 0.59 lbs of waxes and 19.41 lbs of dewaxed oil.
Evaluation of Activity of Tocotrienols to The effects of tocotrienols (in the form of the TItF mixture and individual tocotrienols) on plasma levels of thromboxane Bz and platelet factor 4 in chickens were determined.
These levels are known to correlate with the levels of inflammatory cytokines.
The following feeding conditions were used:
Each group of six chickens (6-week old female white leghorn chickens) was administered a chick mash control diet or a control diet containing one or more additives..
The amount of feed consumed by all groups was comparable to the control group. The feeding period was 4 weeks. The birds were fasted for a period of 14 hours prior to sacrifice (at 0800 hours).
The chicken mash control diet contained the following ingredients:
Ingredient Weight (g) Corn (8.8% protein) 615.0 Soybean Meal 335.0 Corn Oil 10.0 Calcium Carbonate 10.0 Dicalcium Phosphate 20.0 Iodized Salt 5.0 Mineral Mixture 2.5 Vitamin Mixture 2.5 w0 99/25336 PCT/US98/24606 The mineral mixture contained per kg feed: zinc sulfate ~ H20, 110 mg;
manganese sulfate ~ SH20, 70 mg; ferric citrate ~ H20, 500 mg; copper sulfate ~ SH20, 16 mg;
sodium selenite, 0.2 mg; DL-methionine, 2.5 g; choline chloride (50%), 1.5 g;
ethoxyquin (1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline), 125 mg; and thiamine HCI, 1.8 mg.
The vitamin mixture contained per kg feed: vitamin A, 1,500 units; vitamin D3, 400 units;
vitamin E, 10 units; riboflavin, 3.6 mg; calcium panthothenate, 10 mg; niacin, 25 mg;
pyridoxine HCI, 3 mg; folacin, 0.55 mg; biotin, 0.15 mg; vitamin B,2, 0.01 mg;
and vitamin K~, 0.55 mg.
1o Results are reported as mean t standard deviation. Percentages of control are reported in parentheses. The following results were obtained:
Diet Thromboxane Bi (mg/100 Platelet Factor ml) 4 (ag/ml) 1)control diet + 16.7 ~ 1.69 7.2 f 0.48 5% corn oil (100.0) (100.0) 2) control diet + 15.8 t 1.29 7.5 t 0.42 5% corn oil + waxes (50 ppm) (94.6) (104.2) 3) control diet + 12.4 ~ 1.42 5.7 ~ 0.64 5% corn oil + TRF (50 ppm) (74.3) (79.2) Significant decreases of thmmboxane BZ and platelet factor 4 plasma levels were observed in the chickens fed a control diet supplemented with TRF.
w0 99/25336 PCT/US98/24606 Diet Thromboxane B= (pg/ml) Platelet Factor 4 (ng/ml) 1)control diet 25.84 t 0.84 13.9 t 0.49 ( 100.00) ( 100.00) 2) control diet + 20.55 ~ 0.95 9.67 ~ 0.43 TRF (50 ppm) (79.53) (73.87) 3) control diet + 21.6310.89 8.78 t 0.72 a-tocotrienol (50 ppm)(83.71) (67.07) 4) control diet + 19.22 t 0.78 8.36 t 0.79 ~-tocotrienol (50 ppm)(74.38) (63.87) 5) control diet + 18.65 f 0.99 8.23 t 0.77 tocotrienol (50 ppm)(72.17) (62.87) 6) control diet + 16.74 t 1.62 7.76 f 1.67 desmethyl-tocotrienol (50 ppm)(64.78) (59.28) 7) control diet + 16.42 ~ 1.36 7.27 ~ 0.74 P2s tocotrienol (SO ppm)(63..54) (55.54) 8) control diet + 25.46 + 1.52 12.38 t 1.26 Geraniol (100 ppm) (98.53) (94.57) 9) control diet + 24.89 ~ 0.88 12.24 ~ 1.49 Lovastatin (100 ppm) (96.32) (93.51) 10) control diet 24.95 ~ 0.97 12.19 t 1.15 + Geraniol (50 ppm) + Lovastatin(96.56) (93.12) (50 ppm) Significant decreases of thromboxane B2 and platelet factor 4 plasma levels were observed in the chickens fed a control diet supplemented with TRF and individual tocotrienols.
The effects of tocotrienols (in the form of the TRF mixture and individual tocotrienols) on plasma levels of thromboxane B2 and platelet factor 4 in swine were determined.
These levels are known to correlate with the levels of inflammatory cytokines.
The following feeding conditions were used:
Each group of three swine (5-month old swine carrying Lpds and Lpu~ mutant alleles) 1 o were administered a control diet or a control diet supplemented with one or more additives. After a 12 hour fast, plasma samples were taken at 42 days from the start of the feeding period.
The swine control diet contained the following ingredients:
Ingredient Weight Percentage Corn (9.3% protein) 78.37 Soybean Meal (44.0% protein) 15.42 Lard 3.00 Calcium Carbonate 0.95 Dicalcium Phosphate 0.9b Mineral Mixture 0.30 Vitamin Mixture 1.00 The mineral mixture contained per kg feed: zinc sulfate ~ H20, 110 mg;
manganese sulfate ~ SH20, 70 mg; ferric citrate ~ HZO, 500 mg; copper sulfate ~ SH20, 16 mg;
sodium selenite, 0.2 mg; DL-methionine, 2.5 g; choline chloride (50%), 1.5 g;
ethoxyquin (1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline), 125 mg; and thiamine HCI, 1.8 mg.
The vitamin mixture contained per kg feed: vitamin A, 1,500 units; vitamin D3, 400 units;
vitamin E, 10 units; riboflavin, 3.6 mg; calcium panthothenate, 10 mg; niacin, 25 mg;
pyridoxine HCI, 3 mg; folacin, 0.55 mg; biotin, 0.15 mg; vitamin B,2, 0.01 mg;
and vitamin Ki, 0.55 mg.
The gain in body weight in all groups was comparable to the control.
WO 99/25336 PC'T/US98/24606 Results are reported as mean t standard deviation. Percentages of control are reported in parentheses. The following results were obtained:
DIet Thrombo:ane BZ (pg/100Platelet Factor ml) 4 (ng/ 100 ml) I )control diet 75.93 ~ 1.45 24.12 t 1.75 ( 100.00) ( 100.00) 2) control diet + 64.55 t 1.18 20.32 f 1.70 TRF (50 ppm) (95.23) (84.30) 3) control diet + 62.54 ~ 1.39 20.65 t 1.15 Y-tocotrienol (50 ppm)(92.25) (85.28) 4) control diet + 60.48 t 1.46 19.85 t 1.27 desmethyl tocotrienol (50 ppm)(87.66) (82.59) 5) control diet + 57.03 t 1.95 19.15 f 1.45 pis tocotrienol (50 ppm)(83.02) (79.36) Significant decreases of thromboxane BZ and platelet factor 4 plasma levels were observed in the swine fed a control diet supplemented with TRF and individual tocotrienols.
The effects of y-tocotrienol on the release of superoxide in human peripheral blood neutrophils were determined. Neutrophils are an extracellular source of oxygen free radicals and, together with nitric oxide, form peroxynitrite (responsible for endothelial damage). Activated neutrophils attach to endothelial tissue, where they release the potent toxin, superoxide. Superoxide amplifies the inflammatory response and impairs local blood circulation.
The neutrophils tested were isolated by density centrifugation on Ficoll-Hypaque gradients using conventional methods (see E. Serbinova et al., Free Rad. Bio.
and fed., 2o 10, pp. 263-75 (1991)). The neutrophils were then placed in a 96-well plate. y-tocotrienol and phorbol myrstate acetate were added to the wells at the same time. The secretion of superoxide was measured as the superoxide dismutase-inhibitable reduction of ferricytochrome C. The results of this study are displayed in Figure 1.
The amount of released superoxide was reduced from 19.7 nmole (5x105 cells/hour) in the control to 8.0 and 0.0 nmole at y-tocotrienol concentrations of I O'~ and 10'5, respectively.
The following restenosis animal models can be used to evaluate the efficacy of tocotrienols:
1o A.M. Lafont et al., J. Clin. Invest., 95(3), pp. 1018-25 (1995) and LA.
Guzman et al., A_rteriosclerosj~, TlLrombosis & VascLla_r Biol~,v, 16(3), pp. 479-87 (1996) describe rabbit models in which angioplasty was performed on established atherosclerotic lesions.
R. Bonan et al., Catheterization & Ca'rdiovascula_rDi~Q,~, 38(1), pp. 44-49 (1996) 15 describe a balloon injurylreinjury (plaque of dilation) protocol in swine.
ApoE deficient mice develop atherosclerotic lesions that are similar to those in humans.
Using apoE deficient (-/-) C57BL/6 mice, atherosclerotic lesions develop after about 16 weeks when fed a standard low fat mouse diet. ApoE heterozygous (+/-)C57BIJ6mice 20 only develop atherosclerotic lesions when fed an atherogenic high fat diet.
Once atherosclerosis has developed, the mice can be subjected to angioplasty and the effects of tocotrienols can then be measured by administering unsupplemented feed or feed supplemented with between about 5 and about 100 ppm of tocotrienols and tocotrienols mixtures (such as P~8, P25 and TRF15). The effect of the tocotrienols on the prevention of restenosis can be measured using a standard low fat mouse feed or a high fat atherogenic diet. Feeding protocols can be carried out for a period of about 4 to about 40 weeks.
One or more of these models can be used to establish the efficacy of tocotrienol compositions useful in the methods of this invention to prevent restenosis.
While we have described a number of embodiments of this invention, it is apparent that our basic constructions may be altered to provide other embodiments which utilize the compositions and methods of this invention. Therefore, it will be appreciated that the scope of this invention is to be defined by the appended claims, rather than by the specific embodiments that have been presented hereinabove.