Methods in Microbiomics
Set of guidelines and best practices for robust and reproducible bioinformatics processing and data analysiswith the focus on Microbiomics research.
Important
This documentation is currently under construction.
Tutorials
Tutorials are available for each section listed below. To start, create a directory for the tutorial of your interest:
mkdir<section>_tutorialcd<section>_tutorial
For example, forData Preprocessing, run:
mkdirpreprocessing_tutorialcdpreprocessing_tutorial
Note
For the next section, you have to install conda first. Installation instructions can be foundhere.
For each of the sections we have provided a link to a conda environment file (i.e. a file that specifies which packages to install) and a test dataset that can be used for practice. Download these files and save them to the tutorial directory. Next, you can create and activate the conda environment and extract the test data:
condaenvcreate-f<environmentname>.yamlcondaactivate<environmentname>tar-xvpfSample1_isolate.tar.gz
ForGenome Assembly, you need to installmamba. Here are the commands to create the conda environment and unpack the data:
mambaenvcreate-fisolate_assembly.yamlcondaactivateisolate_assemblytar-xvpfSample1_isolate.tar.gz
Now, you are ready to run the example commands given in the corresponding section.
Data Preprocessing
Warning
Before proceeding to any of the bioinformatics workflows, make sure you have good quality data.SeeData Preprocessing for more.
Downloaddatapreprocessingcondaenvironmentfile
anddatapreprocessingtestdataset
Genome Assembly
Best practices forGenome Assembly andMetagenomic Assembly.
Downloadisolateassemblycondaenvironmentfile
andisolateassemblytestdataset
Taxonomic Profiling
Best practises for profiling of amplicon and metagenomic data
Transcriptomics Analysis
Best practices for transcriptomic and metatranscriptomic data analysis
Best practices in metagenomic data analysis
Support
If you have any questions or suggestions leave a comment below!