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Abstract
Two closely related cysteine-rich hydrophobic proteins, Sc3p and Sc4p, of the basidiomyceteSchizophyllum commune are developmentally regulated and associated with the walls of aerial hyphae and fruit-body hyphae. They are present in the walls as hot-SDS-insoluble complexes which can be extracted with formic acid. The hydrophobins can then be dissociated by oxidation with performic acid. However, extraction of the walls with trifluoroacetic acid results in both solubilization and dissociation of the hydrophobin complexes into monomers. This suggests that non-covalent interactions are responsible for formation of these insoluble complexes. Carboxymethylation with iodoacetic acid only occurred after reduction with DTT indicating all cysteines in the monomeric hydrophobins involved in intramolecular disulfide bridges. Abundant proteins with similar properties were found in walls from all other filamentous fungi tested, including the basidiomycetesPleurotus ostreatus, Coprinus cinereus, Agaricus bisporus, andPhanerochaete chrysosporium, the ascomycetesAspergillus nidulans, Neurospora crassa, andPenicillium chrysogenum, and the zygomyceteMucor mucedo.
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Department of Plant Biology, Biological Center, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands
Onno M. H. de Vries, M. Peter Fekkes, Han A. B. Wösten & Joseph G. H. Wessels
- Onno M. H. de Vries
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- M. Peter Fekkes
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- Han A. B. Wösten
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- Joseph G. H. Wessels
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de Vries, O.M.H., Fekkes, M.P., Wösten, H.A.B.et al. Insoluble hydrophobin complexes in the walls ofSchizophyllum commune and other filamentous fungi.Arch. Microbiol.159, 330–335 (1993). https://doi.org/10.1007/BF00290915
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